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<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3D8</accession>
    <name>A0A672Y3D8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-ribosylation factor-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115419879</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SYS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029990804.1">
        <property type="nucleotide sequence ID" value="XM_030134944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y3D8"/>
    <dbReference type="FunCoup" id="A0A672Y3D8">
        <property type="interactions" value="1526"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000271.1">
        <property type="protein sequence ID" value="ENSSORP00005000253.1"/>
        <property type="gene ID" value="ENSSORG00005000191.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419879"/>
    <dbReference type="InParanoid" id="A0A672Y3D8"/>
    <dbReference type="OrthoDB" id="414781at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04160">
        <property type="entry name" value="Arfrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000509">
        <property type="entry name" value="ADP-ribosylation factor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45909:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="201" mass="22577" checksum="9D8416E98E56ADAE" modified="2020-06-17" version="1">MYTLLSGLYKYMFQKDEYCVLILGLDNAGKTTFLEQTKTKFSKNYKGMNLSKITTTVGLNIGTIDVGKARLMFWDLGGQDELQSLWDKYYAESHGVIYVIDSTDEERLSESKEAFEKMISSETLEGVPLLVLANKQDVPNCLSVPDIKTAFSDCAPKIGKRDCLVQPCTALTGDGVNEGIEWMVKCVVRNIHRPPRQKDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3V3</accession>
    <name>A0A672Y3V3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y3V3"/>
    <dbReference type="Ensembl" id="ENSSORT00005000543.1">
        <property type="protein sequence ID" value="ENSSORP00005000518.1"/>
        <property type="gene ID" value="ENSSORG00005000351.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y3V3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12893">
        <property type="entry name" value="SPRY_PRY_TRIM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
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        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
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        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="93"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="280"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="134"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="470" mass="54297" checksum="05CAF7AF6747700B" modified="2020-06-17" version="1">MAKMAEKPILENFLTCPECLEVFRDPVSLSCNHSFCLICLKEFWKEKENRTCPVCRRKDSKGLSVNFALKELAESFAGSLKVGSSETEKGEQKVEVVCSKHPEEPKLFCKDENRAVCLVCDFPHQHEHTVVPVEEAVTEMKDELKSDLKSLQEKKKKYEETQKTYKEIMEHLKNQVLWTESQIRAEFNKLQQFLKEEEESRLAALREEEEQKRRSVMREMKRIEEQMSSVSDSICAVEEELQKASAPFLISCKDTQSRAQCSVSEPQLISGALIHVDKHLGNLSFRVWDKMRDKVHFSPVILDPNTAHGWLHLSDDLTSVRNGDTYQQVPDNPERNNHYASVHGCEGFTSGKHSWDVEVRDHLRWTLGLRYVSPKYGFWCLCHHSGKYTNVLGQTVTVQTSPQRIRVELDYNRGEVSFYNTDDKTQIYTHRDTFTEKLYPYFSVGKAGGAKTTDIKICPIDFSVFIEMRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y563</accession>
    <name>A0A672Y563_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KCTD3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y563"/>
    <dbReference type="Ensembl" id="ENSSORT00005001604.1">
        <property type="protein sequence ID" value="ENSSORP00005001557.1"/>
        <property type="gene ID" value="ENSSORG00005000977.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y563"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000038">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047876">
        <property type="entry name" value="SHKBP1/KCTD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15859">
        <property type="entry name" value="SETA BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15859:SF5">
        <property type="entry name" value="SH3KBP1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="10"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="145" mass="16325" checksum="28FB5B6E831C172D" modified="2020-06-17" version="1">MMSNSARSGDIIHLNVGGKRFSTSRQTLTWVPDSFFSSLLSSRISTLKDETGAIFIDRDPSLFAPILNFLRTKELHPRSINVHMLMHEAEFYGIAPLGQCWCVNFSCVMSWTDHPVEMCCSTVTCLHQVHPTTTTTTITITHLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8N1</accession>
    <name>A0A672Y8N1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Organic cation transporter-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">slc22a18</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May act as a transporter of organic cations based on a proton efflux antiport mechanism. May play a role in the transport of chloroquine and quinidine-related compounds in kidney. Plays a role in the regulation of lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y8N1"/>
    <dbReference type="Ensembl" id="ENSSORT00005000145.1">
        <property type="protein sequence ID" value="ENSSORP00005000129.1"/>
        <property type="gene ID" value="ENSSORG00005000055.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8N1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17331">
        <property type="entry name" value="MFS_SLC22A18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000297">
        <property type="entry name" value="Solute carrier family 22 member 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24002">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24002:SF3">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="22"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00078639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="418" mass="45459" checksum="A9EF50CE11DC6DBA" modified="2020-06-17" version="1">FLRVGNGSGSKVRIRPGELWQRTTVIRIIYLIAALDITWMFVQFSVTPYLAKKLGFDTLWFGYLQTMVGVAQLIGGPMFGRFADVFGARAALSLACAATVVFFVLLAIAEYPIMLFIHKLPTVFMHVLPASQMVVTDLTEPEKRADALSKLGLCFGVGMIAGSTLGGHLNTRYGERFTACFGAVGSAVSLMLVLRFIPKTTKVQHPRTSTDGENKNKSIFNMGEITRLLKFPGVAPTFTVKIIAGLPSGIFQVMFSIIALEFFKLQPEQNGYLMAYFGIVQMVIQGGVIGRLTARFSENSLLNLSIAVAALVGLAQVCADNVFQFCVTVVPMMFSLSVFNVITDSILTKSVPSSDTGTMLGLCASVQSLLRTVGPTVGGFLYVNFGVSSIGFIQFVVNAAVFMFLLLRPHKKTSEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCN1</accession>
    <name>A0A672YCN1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YCN1"/>
    <dbReference type="Ensembl" id="ENSSORT00005001468.1">
        <property type="protein sequence ID" value="ENSSORP00005001425.1"/>
        <property type="gene ID" value="ENSSORG00005000869.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCN1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025466334" description="Peptidase S1 domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="36"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="93" mass="9821" checksum="D951955BBD43D87B" modified="2020-06-17" version="1">MPPRHAVNSSVMTSLAVRLTAVCSTDCGSGLNSSRALGAQQAAPGSWPWQVSLQVDGSHRCGGAIISPYWIVTAAHCVGLQSWKLGRVCRDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YD04</accession>
    <name>A0A672YD04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Lysine-specific demethylase 8</fullName>
            <ecNumber evidence="3">1.14.11.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">JmjC domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Jumonji domain-containing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115424961</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Histone demethylase required for G2/M phase cell cycle progression. Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator. May play a role in the regulation of the circadian clock.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="3"/>
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    <dbReference type="FunCoup" id="A0A672YD04">
        <property type="interactions" value="809"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000762.1">
        <property type="protein sequence ID" value="ENSSORP00005000736.1"/>
        <property type="gene ID" value="ENSSORG00005000454.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Bifunctional peptidase and arginyl-hydroxylase JMJD5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM_KDM8_N"/>
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        <property type="entry name" value="Cupin_8"/>
    </dbReference>
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        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
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        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND ARGINYL-HYDROXYLASE JMJD5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="ARM_KDM8_N"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="266"/>
            <end position="402"/>
        </location>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00059090"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00082907"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="402" mass="46075" checksum="5C028098253FB5FB" modified="2020-06-17" version="1">MTALWSKLSAVLPPNEEEFPLQFSDKVESSVVSVLQQSRRQLYSDATSTDRTLTAQIILDLSWEKLNTGTWRDVDKEWRCVYSYGCLFKVAALCREHPSADEILQGIRTCDMGLLMGAAIMDNVLQVLVQILQEEVRKSTKEDEQHNITSTFRVKTERPRVPVIKDEQAVPRIKCPSLESFKSDYLLPLRPVILEATADHWPAFNEHPWSVEYLRSVAGCRTVPVEVGSRYTDEEWSQTLLTVNEFIDQYDGAKAVGYLAQHQLFDQIPELKEDIRIPDYCCLGDGDEDDITVNAWFGPGGTVSPLHQDPQQNFLVQVVGSKYIRLYSPEDTDKLYPHQSSLLHNTSQVEVENPDTERFPEFARAPYLECVLQPGDVLFIPVQHWHYIRSLELSFSVSFWWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDC7</accession>
    <name>A0A672YDC7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:ch211-137a8.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        </location>
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            <end position="151"/>
        </location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="407"/>
        </location>
    </feature>
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            <end position="435"/>
        </location>
    </feature>
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    </feature>
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        <location>
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            <end position="525"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="545"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001860.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="574" mass="59695" checksum="6ACC187BEF844873" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHL9</accession>
    <name>A0A672YHL9_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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    <reference evidence="9" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome 11"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:transcription regulator complex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
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            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="8">
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    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
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            <end position="308"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
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        <location>
            <begin position="393"/>
            <end position="418"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
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        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
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        <location>
            <begin position="44"/>
            <end position="60"/>
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    </feature>
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            <begin position="87"/>
            <end position="101"/>
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    </feature>
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            <begin position="137"/>
            <end position="148"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        <source>
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            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="418" mass="48115" checksum="228C76E9AB57AD7A" modified="2025-10-08" version="2">MNVQMGHLLVVKEEVLPEWEWNPIVDQKEPEPEPPHIKEEQEELWTSQETDITKFPSTPENKVDCGGSEPASSFHPHRHLQRKTFEQNEDSEETEDSDEDQTLNHKPESKPPTNSSSEQMETETDGDDSGGSEPDINLTTLTQETNSPRDMMKNTDWTLSDGGCEKKLYDCSVCSKTFCRKSSLKTHMRIHTGERPFCCSVCGKKFTQKSTLSNHMTSHMGEKPFRCSLCHKCFGARKNISQHMRVHTGERSFNCSVCGKRFTSKSNLTAHMKVHTGEKPFSCSECSKRFTWKIDLERHMSLHTGEKPFSCSECSKRFTWKIDLERHMRLHTGEKPFSCSECGKRFTQKTSVECHMRLHTGEKPFSCSECGQTFTQRTGLKYHMRLHTGETPFSCVVCQKSFKLKHHLQAHMKIHTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIA3</accession>
    <name>A0A672YIA3_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Solute carrier family 26 member 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">slc26a6l2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005004268.1"/>
        <property type="gene ID" value="ENSSORG00005002254.1"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
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        <property type="entry name" value="STAS domain"/>
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        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
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        <property type="entry name" value="SLC26A/SulP_fam"/>
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        <property type="entry name" value="STAS_dom_sf"/>
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        <property type="entry name" value="sulP"/>
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        <property type="entry name" value="SULFATE TRANSPORTER"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SLC26A"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="122"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="333"/>
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            <end position="758"/>
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        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="776" mass="85504" checksum="5896C826CE6B7EA6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIS4</accession>
    <name>A0A672YIS4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP-binding protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gtpbp8</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029981689.1">
        <property type="nucleotide sequence ID" value="XM_030125829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YIS4"/>
    <dbReference type="FunCoup" id="A0A672YIS4">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005004599.1">
        <property type="protein sequence ID" value="ENSSORP00005004466.1"/>
        <property type="gene ID" value="ENSSORG00005002708.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413072"/>
    <dbReference type="CTD" id="29083"/>
    <dbReference type="InParanoid" id="A0A672YIS4"/>
    <dbReference type="OrthoDB" id="391988at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000857">
        <property type="entry name" value="GTP-binding protein 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052279">
        <property type="entry name" value="EngB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498:SF1">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EngB-type G" evidence="8">
        <location>
            <begin position="105"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="297" mass="33546" checksum="33EA857CDABE1337" modified="2020-06-17" version="1">MFSLQLRLWLHPGAQVCVVSRLKAHRLASLQNTVALPQKKTQSLLYPFSQVEPYLDRSIDRTQFQLFHPSVEDMLKAEKLFCSSPSHKIDYYISAERMDHAPVMKQPEVCFIGRSNVGKSSLIKALFSLTPEVEVRVSKTPGHTKKMNFFRVGKAFTIVDMPGYGHKAPKDFVDMVEPYLYSRKNLVRTFLLVDGSVGLQKADLIALEMCEEIRRPYVMVVTKIDKGTRGVLLSNLMHLQDAVKTHTSSCFPQPFLLSSLNFWGIYLLRCFIAHITGSITLTTPPTNSNEGATAHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIY8</accession>
    <name>A0A672YIY8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Multidrug and toxin extrusion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115427055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YIY8"/>
    <dbReference type="Ensembl" id="ENSSORT00005004653.1">
        <property type="protein sequence ID" value="ENSSORP00005004519.1"/>
        <property type="gene ID" value="ENSSORG00005001754.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YIY8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="500" mass="54432" checksum="E2AECD455A0A9619" modified="2020-06-17" version="1">VGADTTTKHFLHVISQLMIFMISFISTVFCGHLGKTELASVALAIAVVNVTGVSIGTGLSLTCDTLISQTYGSGNLKRVGVILQRGVLILLLACFPCWAVLINTETLLLAVKQSPEVASLSQLYVKIFMPALPAAFMYQLQGRYLQNQGIIWPQVITGAIGNVLNAIINYVFLYPLDMGVAGSAAANAISQYLLAVVLFIYIYWKGLHKATWGGWSLDCLQEWGPFIQLAIPSMLMLCLEWWMFEIGGFLAGVISETELGAQSIVYELSVVAYMFPLGFAAAASVRVGNALGAGNVEQAKLSCKVPIICSCKTYFESIKNLNLHVFRDILQRVADVMIIFAFMHLADAVAGVTGGVLRGAGKQMIGALCNLVGYYFIGFPIGASLMFAAKMGIVGLWTGLSLCVLMQMIFFIVFVFKLDWKKAAEEVCIHCLNKESADRTLSVVICTVEQRCFQPGLKHCHSRGLSHVIRKTVPEISCIELKCSFTVFSPDSGHQEKTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJG1</accession>
    <name>A0A672YJG1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarylacetoacetate hydrolase domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fahd2a</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YJG1"/>
    <dbReference type="Ensembl" id="ENSSORT00005004847.1">
        <property type="protein sequence ID" value="ENSSORP00005004711.1"/>
        <property type="gene ID" value="ENSSORG00005002863.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJG1"/>
    <dbReference type="OrthoDB" id="411064at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="115"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="322" mass="35564" checksum="0D79423A7EF2DA94" modified="2020-06-17" version="1">MRLVRPLHILKNPFSQRQTQNLARRGLSGAAMRLVQFKRLSDGGGGEVRVGVEQGEGLDVVDLKAFDPSMPSTMKELLELGDQGLDRAQRAVASGQCVLKRSDVRLLSPILNPEKVVCVGMNYRDHCLEQNAPIPKEPIIFSKFPSAITGPYDDIVLPSESQEVDWEVELAFVIGRKGKHIKEEDALSHVAGFTVANDVSARDWQMKRNGRQWLLGKTFDSFCPLGPALVTTDAVQEPHRLDIRCLVNGDTVQSSNTDQMIFNTQTLIAWVSKFVTLTPGDVFLTGTPPGVGVFRKPPVFLKRGDVVECHIQQLGSIINKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMQ1</accession>
    <name>A0A672YMQ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Plays a role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">SEC16A and SEC16B are each present in multiple copies in a heteromeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">Belongs to the SEC16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YMQ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005006055.1">
        <property type="protein sequence ID" value="ENSSORP00005005808.1"/>
        <property type="gene ID" value="ENSSORG00005003292.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YMQ1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051668">
        <property type="term" value="P:localization within membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09233">
        <property type="entry name" value="ACE1-Sec16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1030:FF:000002">
        <property type="entry name" value="Protein transport protein sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024340">
        <property type="entry name" value="Sec16_CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF13">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12932">
        <property type="entry name" value="Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="14 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0492">Microsome</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="19">
        <location>
            <begin position="149"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="18">
        <location>
            <begin position="303"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="40"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="635" mass="70795" checksum="17F03453546B591A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQ30</accession>
    <name>A0A672YQ30_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pou4f4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YQ30"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006943.1">
        <property type="protein sequence ID" value="ENSSORP00005006683.1"/>
        <property type="gene ID" value="ENSSORG00005003899.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="6358449at2759"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF44">
        <property type="entry name" value="BRAIN-SPECIFIC HOMEOBOX_POU DOMAIN PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="12">
        <location>
            <begin position="191"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="284"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="286"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="350" mass="38466" checksum="F7A68891676C8A18" modified="2020-06-17" version="1">MMSMNSKQPFSMHPILHEPKYTPLHSSSEAIRRACLPTPSLQGNIFAGFDETLLQRAEALAAVDIVAQKSHPFKPDATYHTMTTMTSMTCTPTSSSAHLHHPSVLTSHHHPAHHQPAQGLEGDLLDHLTPGGISLGGMPGSDVCSTASHTAHAAHMSAINHMQHHHHHPQSMNMHPHGLGSHTSLGAAGGDAEPDPRELESFAERFKQRRIKLGVTQADVGAALANLKIPGVGCLSQSTICRFESLTLSHNNMVALKPILEAWLEEAERAQREKMSKPEIFNGGDKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQX6</accession>
    <name>A0A672YQX6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cellular retinoic acid-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cellular retinoic acid-binding protein I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">crabp2b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YQX6"/>
    <dbReference type="Ensembl" id="ENSSORT00005007253.1">
        <property type="protein sequence ID" value="ENSSORP00005006991.1"/>
        <property type="gene ID" value="ENSSORG00005004038.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YQX6"/>
    <dbReference type="OrthoDB" id="195110at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016918">
        <property type="term" value="F:retinal binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0845">Vitamin A</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="8">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="148" mass="16411" checksum="8405EB28B4B68594" modified="2020-06-17" version="1">METCDAVEKTITDFSGKWKMKSSENFEELLKALGVNVFLRKIAVTAASSPAVEISQQEESLSIKTSTSVRTTHVSFTVGQSFNETTVDGRPCTSFPKWETDSKISCEQTLQKGEGPKTSWTRELTNDGELILTMTAGDVVCTRVYERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSH1</accession>
    <name>A0A672YSH1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YSH1"/>
    <dbReference type="Ensembl" id="ENSSORT00005007804.1">
        <property type="protein sequence ID" value="ENSSORP00005007524.1"/>
        <property type="gene ID" value="ENSSORG00005004289.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YSH1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="53" mass="6228" checksum="47C5B18E7D091B9D" modified="2020-06-17" version="1">MCHRKCNLIIIWNSALYISYSNSLHDMKPCFCNLTSELLADEACTCNYEKYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YU33</accession>
    <name>A0A672YU33_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cyclosome subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">anapc2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5. In the context of the APC/C complex, directly interacts with UBE2C and UBE2S. Interacts (via cullin domain) with ANAPC11 and with UBCH10. Interacts with NEUROD2. Interacts with FBXO43; the interaction is direct.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YU33"/>
    <dbReference type="FunCoup" id="A0A672YU33">
        <property type="interactions" value="1715"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008285.1">
        <property type="protein sequence ID" value="ENSSORP00005008002.1"/>
        <property type="gene ID" value="ENSSORG00005003817.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YU33"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000284">
        <property type="entry name" value="Anaphase-promoting complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000027">
        <property type="entry name" value="Anaphase-promoting complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000008">
        <property type="entry name" value="anaphase-promoting complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014786">
        <property type="entry name" value="ANAPC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08672">
        <property type="entry name" value="ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01013">
        <property type="entry name" value="APC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="15">
        <location>
            <begin position="499"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="458"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="821" mass="93620" checksum="DF66D5C45B93C306" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYR4</accession>
    <name>A0A672YYR4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD repeat domain-containing protein 83</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mitogen-activated protein kinase organizer 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">wdr83</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat MORG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029981051.1">
        <property type="nucleotide sequence ID" value="XM_030125191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YYR4"/>
    <dbReference type="FunCoup" id="A0A672YYR4">
        <property type="interactions" value="802"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010186.1">
        <property type="protein sequence ID" value="ENSSORP00005009856.1"/>
        <property type="gene ID" value="ENSSORG00005005386.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412344"/>
    <dbReference type="CTD" id="84292"/>
    <dbReference type="InParanoid" id="A0A672YYR4"/>
    <dbReference type="OrthoDB" id="71437at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000273">
        <property type="entry name" value="WD repeat domain-containing protein 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="35044" checksum="8A1E3157BA7F6BE1" modified="2020-06-17" version="1">MAFPQPRPQAPQLPQHLLRTIDCQQGAVRAVRFNVDGNYLLSCGSDKSLKLWTVSRGTLLKTYSGHGYEVLDADGSYDNSQLCSCSSDKTVILWDVATGQVTRKLRGHAGKVNCVQFNEEATVILSGSIDGTVRCWDTRSRRFEPIQVLDEARDSISSLKVAQHELLTGSVDGRVRRYDLRMGQLHVDFIDSPITCVCFSQDGQCTLSSSLDSAVRLLDKSTGEMLGEYKGHKMKGYKLDCCLSSKDTHVLSCSEDGHVYCWDLVEGSLSLKLPVGKAVVQSLSFHPSETRLLTAMEGRVQVWGSEPEETMDDPTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZF8</accession>
    <name>A0A672YZF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CXXC-type zinc finger protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CpG-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">PHD finger and CXXC domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cxxc1b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZF8"/>
    <dbReference type="Ensembl" id="ENSSORT00005010320.1">
        <property type="protein sequence ID" value="ENSSORP00005009990.1"/>
        <property type="gene ID" value="ENSSORG00005003692.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZF8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15553">
        <property type="entry name" value="PHD_Cfp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000138">
        <property type="entry name" value="CXXC-type zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022056">
        <property type="entry name" value="CpG-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF4">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12269">
        <property type="entry name" value="CpG_bind_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="18">
        <location>
            <begin position="159"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="242"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="585" mass="68578" checksum="E34E67F165723155" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0X8</accession>
    <name>A0A672Z0X8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Gamma-aminobutyric acid (GABA) A receptor, delta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC115423064</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
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    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
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        </source>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010791.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Neoteleostei</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013426.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="109" mass="11828" checksum="971DCCA51CC8F40E" modified="2025-10-08" version="2">MEDAGFHTIEAVAYAPKKELLNIKGISEAKADKILGEAAKLVPMGFTTATEFHQRRAEIIQISTGSKELDKLLQGGIETGSITEMFGEFRTGKTQLCHTLAVTCQVQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z800</accession>
    <name>A0A672Z800_9TELE</name>
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        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
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    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115431184</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
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    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSSORG00005006983.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z800"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="46"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="316" mass="36540" checksum="0908922146F719D9" modified="2020-06-17" version="1">MSGYELIMKNYSQVSYFNLTAYFDTGTLKYMYFILVLSLYLSVIAANVLLIVLICVNRSLHEPMYLFLCSLFVNELFGSSAVYPVLLLQILRDIHTVSAPFCFLQIYTGHTYACVEYTLLAIMSYDRYLAICYPLQYNQCMASQKVFLLVALSWFYPFILIIILISLSAPLQRCGNIIHKVYCDNYSIVKLACSDTTVSNIYGLLMTVMVVFIPLFFILYTYMRILKVCFSGSKQTRHKAVSTCTPHLASVINYCFGVCFEVFQSRFNMDNVPNMLRIFLSLYWLTCQPLFNPLMYGLNMSKIRIICKNLFLFRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z864</accession>
    <name>A0A672Z864_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z864"/>
    <dbReference type="Ensembl" id="ENSSORT00005013074.1">
        <property type="protein sequence ID" value="ENSSORP00005012677.1"/>
        <property type="gene ID" value="ENSSORG00005006630.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z864"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000053">
        <property type="entry name" value="zinc finger protein 182 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF70">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="46"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="275" mass="30022" checksum="44D1CBA6E051FDB2" modified="2020-06-17" version="1">MTTTLLNINISFHINKTSSVITIFVLIDCLLLLLKKLYSSSCANKAQSNKNDGACNATSTSEQQKKNNVETASNGSAKSTAVNKCPSQNGTSAGSKALTSSKRPNDGGTTSDGGAGPPPKKKHIDEARKDSQPDNSSTSNGNNNTPSSSQKTSCKDPYKCKLCGKIKGNFNTKKILTESHTAEKNIKCDTCGLMFSETWDLNKHLVIHLADKPFRCKVCGKGFNRRFNLHMHERVHTGEDKPYSCATCAKSFASILYSRTRNNDKKYQIKVCIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8D2</accession>
    <name>A0A672Z8D2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc transporter ZIP4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Solute carrier family 39 member 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Zrt- and Irt-like protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">slc39a4</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Selective transporter that mediates the uptake of Zn(2+). Plays an essential role for dietary zinc uptake from small intestine. The Zn(2+) uniporter activity is regulated by zinc availability. Also exhibits polyspecific binding and transport of Cu(2+), Cd(2+) and possibly Ni(2+) but at higher concentrations.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z8D2"/>
    <dbReference type="Ensembl" id="ENSSORT00005013493.1">
        <property type="protein sequence ID" value="ENSSORP00005013085.1"/>
        <property type="gene ID" value="ENSSORG00005006788.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z8D2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049406">
        <property type="entry name" value="ZIP4_12_EF-hand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041137">
        <property type="entry name" value="ZIP4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF21">
        <property type="entry name" value="ZINC TRANSPORTER ZIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21116">
        <property type="entry name" value="EF-hand_Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18292">
        <property type="entry name" value="ZIP4_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 23" id="KW-0732">Signal</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0864">Zinc transport</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025693954" description="Zinc transporter ZIP4" evidence="23">
        <location>
            <begin position="23"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="593"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="624"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc transporter ZIP4 N-terminal" evidence="24">
        <location>
            <begin position="51"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc transporter ZIP4/12 EF-hand" evidence="25">
        <location>
            <begin position="217"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="247"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="250"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF18292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF21116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="653" mass="71905" checksum="114C6088A7DB7141" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBX5</accession>
    <name>A0A672ZBX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SNARE-associated protein Snapin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Biogenesis of lysosome-related organelles complex 1 subunit 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">snapin</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNAPIN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002600.1">
        <property type="nucleotide sequence ID" value="XM_030146740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZBX5"/>
    <dbReference type="FunCoup" id="A0A672ZBX5">
        <property type="interactions" value="627"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014412.1">
        <property type="protein sequence ID" value="ENSSORP00005013993.1"/>
        <property type="gene ID" value="ENSSORG00005007170.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427978"/>
    <dbReference type="CTD" id="23557"/>
    <dbReference type="InParanoid" id="A0A672ZBX5"/>
    <dbReference type="OrthoDB" id="5399166at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000300">
        <property type="term" value="P:regulation of synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017246">
        <property type="entry name" value="Snapin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028119">
        <property type="entry name" value="Snapin/Pallidin/Snn1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31305">
        <property type="entry name" value="SNARE-ASSOCIATED PROTEIN SNAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31305:SF2">
        <property type="entry name" value="SNARE-ASSOCIATED PROTEIN SNAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14712">
        <property type="entry name" value="Snapin_Pallidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037631">
        <property type="entry name" value="Snapin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR037631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="14608" checksum="278FFE690A87116C" modified="2020-06-17" version="1">MAAATLIETSSSRDAIAEGLLDLLKPAIQQLDLHVHSVRESQVELREHIDNLATELCRINEHQKVALDLDPYVKKLLNARRRVVLVNNILQNAQERLRRLNHNVAKETARRKTMLEASGVFTSRSPSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDZ4</accession>
    <name>A0A672ZDZ4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Microtubule-associated protein, RP/EB family, member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mapre2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDZ4"/>
    <dbReference type="FunCoup" id="A0A672ZDZ4">
        <property type="interactions" value="1658"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015520.1">
        <property type="protein sequence ID" value="ENSSORP00005015059.1"/>
        <property type="gene ID" value="ENSSORG00005007658.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDZ4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
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        <source>
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    </evidence>
    <sequence length="337" mass="38001" checksum="4E4EF5028CBBAE6E" modified="2020-06-17" version="1">MPGPTQALSPNGENNNDIVQDNGTNIIPYRKNTVRGERAYSWGMAVNVYSTSITQETMSRHDITAWVNDILCLNYTKVEQLSSGAAYCQFMDLLFPGCISLKKVKFQAKLEHEYIHNFKLLQASFKRMNVDKIIPVEKLVKGRFQDNLDFIQWFKKFFDANYDGKEYDPVEARQGQDAIPPPDPGEQIFNLPKKSHHAASSPTAGASRSTSTTPKSSTPTSRPSSAKKIPAAPTPAKGEKELEAQVTHLTEQVNTLKLALEGVEKERDYYFSKLREVELLCQEQGEESAPFVDRLMEVLYSSDDQERVGELAEGDAQEVDEQAHEVPDDQQEEQDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFU4</accession>
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        <recommendedName>
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        </recommendedName>
    </protein>
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        <name evidence="7" type="primary">cnbpb</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="161" mass="17649" checksum="17EA284A0B58A753" modified="2020-06-17" version="1">MSSNECFGCGRSGHWVKNCPGAGRGRGKGRGRGKDLFCYRCGELGHVARDCERTEDACYNCGRGGHISRDCKEPKKEREQLCYNCGKAGHMARDCNHAHEQKCYSCGGFGHIQKCCEKVKCYRCGEIGHVAVHCSKASELNCYNCGKSGHLAKECTIEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGB4</accession>
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        <recommendedName>
            <fullName evidence="6">Proteolipid protein 2</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="primary">plp2b</name>
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    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in cell differentiation in the intestinal epithelium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZGB4"/>
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        <property type="gene ID" value="ENSSORG00005008132.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Marvel"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF4">
        <property type="entry name" value="PROTEOLIPID PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
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        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
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            <begin position="18"/>
            <end position="138"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039459"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016080.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="152" mass="16564" checksum="BCEE354AD4449FA6" modified="2020-06-17" version="1">MADTTSSGPSPLERMKSYVKTQKGFILAAEIILSFIILICYAASVYGGYTAVAICEMVFTIIFFVVFMMELDKQFTVVSWIWSDLIRACVGALVYIITSLICVIGGSGDGARIAGGVFGLIAGILFAYDAYTVYLQLKSSRQHQPTSTDDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZIF3</accession>
    <name>A0A672ZIF3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family with sequence similarity 222 member A</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZIF3"/>
    <dbReference type="Ensembl" id="ENSSORT00005016779.1">
        <property type="protein sequence ID" value="ENSSORP00005016273.1"/>
        <property type="gene ID" value="ENSSORG00005008228.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZIF3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029340">
        <property type="entry name" value="FAM222"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16070:SF2">
        <property type="entry name" value="PROTEIN FAM222A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16070">
        <property type="entry name" value="PROTEIN FAM222A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15258">
        <property type="entry name" value="FAM222A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="415" mass="44387" checksum="B99E25FF13736487" modified="2020-06-17" version="1">RSPAKSLRSTGQEKAKHSVCVCSSFSGEPASMVSRYPSPAELDAFAHKTASSPLSIKIFPSDVRVPQHKQLNKTVNGLDTTGQRYSQYSQPYSGGYQGLLAIVKASVGVRGVLKNCEGKRTKHGNNQSSVAPYSNPLNNGVRHGQKTYHSSSCKPPDVPIDTLCSSAGMASGDPSLGPRSELAKVQSLMRHMSRIPHSQAPEPRGGGGPSQPVCAGRGRRGSFPLLGLCRRSATPDTDMAKAAYLDKGDYSTWKQKHHHMQAPYQQGAVRMYGVGNGSVQGHGAVERGGASSLTCATVTGDFPVAPYFTPLWDNMVSTPNSDCYTSQVLATGICAARPRDLAYSADQNLTCGLPSSGLCHAAVLSSSLQSLECLIGEIHPPCIKERMLGRGYEAMGMPLLLEHHQHTNIQLPVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZR05</accession>
    <name>A0A672ZR05_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZR05"/>
    <dbReference type="Ensembl" id="ENSSORT00005019348.1">
        <property type="protein sequence ID" value="ENSSORP00005018797.1"/>
        <property type="gene ID" value="ENSSORG00005009250.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZR05"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="104"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="260" mass="29223" checksum="9E4A64572A9752FF" modified="2020-06-17" version="1">RFHWCTEYRNVVTKTVHVGEDVTLICPRQSSVSGYLFWVRVVAGNFPEALGATYTFDHALVNTTPRITTKQESGTFVLSIKRTTLTDTALYYLSKAFHFIFPEPESHITAIIQDPVSDPLHPGDSEILQYSVLYNLEKKTCPGDPSVYWFRTGSDKSHPTIFYTHGNRSEKCEMRSETHSLKNCTYGLNKSISSSDSGTYYCAVAACGDIFLGNGTKVHVKAVSLTVIAFLIYIMKKNKGNKCHLLFICQVNLQCVLMFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZT62</accession>
    <name>A0A672ZT62_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="9">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ddx10</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="9">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX10/DBP4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="9"/>
    <dbReference type="RefSeq" id="XP_029988800.1">
        <property type="nucleotide sequence ID" value="XM_030132940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZT62"/>
    <dbReference type="FunCoup" id="A0A672ZT62">
        <property type="interactions" value="1908"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020146.1">
        <property type="protein sequence ID" value="ENSSORP00005019587.1"/>
        <property type="gene ID" value="ENSSORG00005009614.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418454"/>
    <dbReference type="CTD" id="1662"/>
    <dbReference type="InParanoid" id="A0A672ZT62"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17941">
        <property type="entry name" value="DEADc_DDX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000874">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001089">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="108"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="308"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
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</entry>
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        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="ZnF_transloc-Spindlin_int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34589">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 2700081O15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34589:SF2">
        <property type="entry name" value="ZINC FINGER TRANSLOCATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18658">
        <property type="entry name" value="zf-C2H2_12"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="498" mass="57169" checksum="045F0A8C15706A32" modified="2020-06-17" version="1">YYHEYWRSEYLMDFDPQRHGMICMVCGSSLATLKLSTIKRHIRQKHPDSLLWSTADKEVIRSGWESHLSLGGGQSLSPHSEEGEAPQPQDQDQDQDQDQDQDVAGPSARTLERYLNDSLHAWFRQEFLMEYEAEAGRLLCMVCGGELPSLHLDHIKRHVLDTHPNSLVYSSEEKHCILQAWSSFPALSCLFYLFVYLELYCQDISKKSRKIDSGADLFAQDVEAIQINSDLYPEGDGTLTQDTCLIGEDGSVGAPQQGPQPLRQPRKRRLRGGDPWRLRLDYLVAYGPQGRGTYCMVCSQVLHDTKVSSFRRHIQESHPETTTLSRQEREAMAAAWTKDYSSEGTQSQEGETQIQVCSHELWIRYLQGSHGFLAQRVEKNVKVRQYDLLEVEPPPPSLYQRRNYQTRWRTEFLMDYDCRRHGLICMVCGATLATLKVSTIKRHIQQVHPRSLYYSGEEKQEALLSYNQTALHFIHSDDCFSQDHGHTELAPTPAHFGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZX00</accession>
    <name>A0A672ZX00_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PLA2c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115416477</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSSORG00005010155.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium-dependent phospholipid binding"/>
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        <property type="term" value="P:glycerophospholipid catabolic process"/>
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    </dbReference>
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        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
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    </dbReference>
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        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
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        <property type="entry name" value="LysoPLipase_cat_dom"/>
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        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF32">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 BETA"/>
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    </dbReference>
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        <property type="entry name" value="PLAc"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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            <begin position="48"/>
            <end position="592"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51210"/>
        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="592" mass="66859" checksum="4858B6D31DA94163" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZN2</accession>
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        <recommendedName>
            <fullName evidence="9">Pleckstrin</fullName>
        </recommendedName>
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        <name evidence="7" type="primary">plek</name>
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    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="component" value="Chromosome 15"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="DEP_dom"/>
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        <property type="entry name" value="PH_domain"/>
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        <property type="entry name" value="Pleckstrin"/>
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        <property type="entry name" value="PLECKSTRIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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            <begin position="20"/>
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            <begin position="10"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="40953" checksum="162A764B8965CD1C" modified="2020-06-17" version="1">MLYLILVWMQSSLISYILRKGTVLNSWNAVWVVLSEDGVEFYKKKTDRSPKGMIPLKGATLVSPCQDYGKRTLVFKITTDKKQDHFFQASHVEERENWVKDIKRAITCLAGGRKFARKSTRRSIRLPETVNLNELYALMRDPDDGVKELKLEQEKRVFNHCFTGATVVDWLISKEKARNRPEALMLATGLLNEGFLQPAGDLSKEGADSREQSVLLDQPDALYYFADSGFFCEGYSSDEDELVKEEFRGNIIKQGCLLKQGHRRKNWKVRKFILRDDPAYLHYYDPTKGDDPLGSIHLRSCVVTAVEYVPDAKKYDIDGNLFEIITSEETHYYLQAATADERKEWIKAIQAVSKSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0R7</accession>
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        <submittedName>
            <fullName evidence="3">GDP-mannose pyrophosphorylase Aa</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">gmppa</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A0R7"/>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005022178.1"/>
        <property type="gene ID" value="ENSSORG00005010820.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="2">
        <location>
            <begin position="78"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="215" mass="23511" checksum="9720CC67C7926270" modified="2020-06-17" version="1">SLEEPTNGWHRAEAIRLEQDIFTALAGHNKLYVYKTLSFWSQIKSAGSAIYASRLYLNQYHTTHPERLASDTEGGPKIRGNVYIHPTANIDPTATLGPNVSIGTGVSIGAGVRVRESIILHGATLQDHCCVLNSIVGWDSTIGKWARVEGTPSDPNPNDPYAKIDSETLFRDGKLTPSITILGCNVSIPSEVIILNSIVLPHKDLNRSFKNQIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A447</accession>
    <name>A0A673A447_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">KIAA0930</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">kiaa0930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A447"/>
    <dbReference type="FunCoup" id="A0A673A447">
        <property type="interactions" value="267"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024074.1">
        <property type="protein sequence ID" value="ENSSORP00005023398.1"/>
        <property type="gene ID" value="ENSSORG00005011321.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A447"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019141">
        <property type="entry name" value="DUF2045"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477:SF13">
        <property type="entry name" value="KIAA0930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477">
        <property type="entry name" value="ZGC:172139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09741">
        <property type="entry name" value="DUF2045"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49360" checksum="6F914D6D6D9C1A48" modified="2020-06-17" version="1">MASFPGLCKAVGPAEEENGELDQSLQQMLKAITDERNRLNIRQEISGLGCFKDDRIVFWTWMFSTYFMEKWAPRQDDMLFYVRRKLSYVSADNTEGKKVEVEVYRRDSKKLPGLGDPDIDWEESVYLNLILQKLDYVVTCAVCTRSDAGDIHIHKKKCQEVFASPSKHAMDSKGEESKMSYPNIFFMIDNFEEVFSDMTVGEGEMVCVELVASDKSNTFQGVIFQGSIRYEALKKVYDNRVSVAAKMAQRMSFGFYKYNNMEFVRMKGPQGKGHAEMAVSRVPTGDTSPYGTEEDQVSPMHERVTSFSTPPTPERNRPSFFSPSLRRKMPRNRIAEMKKSHSANDSEEFFREEEDEDLHTTTNLRSRSLSGTGRSLVGSWLKLNRTEDFFLLYSHLTYVTLPLHRITTEFGYVRKPLALRFTSEPLVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5D2</accession>
    <name>A0A673A5D2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Keratin, type II cytoskeletal 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115422079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993999.1">
        <property type="nucleotide sequence ID" value="XM_030138139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A5D2"/>
    <dbReference type="Ensembl" id="ENSSORT00005024552.1">
        <property type="protein sequence ID" value="ENSSORP00005023852.1"/>
        <property type="gene ID" value="ENSSORG00005011520.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422079"/>
    <dbReference type="InParanoid" id="A0A673A5D2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF9">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="71"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="124"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="273"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="397" mass="45805" checksum="A2FCC9666844173D" modified="2020-06-17" version="1">MSIRNTVVNRSRAQPTKAGFSSMSLGSYSIPRISTGTNQVPQITAVRVNRSLLTPLNVDIDPTIQSVRTQEKDQIKTLNNRFASFIDKVRFLEQQNKMLETKWKLLQGQTTATSNVEPMLKSYIANLQRQLECLNSDKHRLEAENDGMHRNVEDYKTKYEDEINRRNDAENEFVMLKKDVDAGYLSKVDLDEKLTSLNEEVTFLRALYEMELRELQDSLKDTSVVVQMDNARGLNMDQIVSEVKAQYEDIAARSRDEAETWYKKKFSQMTTEADQYGNELRTTKGEIAELNRMIRRLQNEIEAVKTQRASLEKQIAEAEEHGEDAIRDANARIKELEVALQRAKQDMARQVREYQELMNVKLALDIEITTYRKLLEGEEERLGQQSVVGIHTVPSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8F9</accession>
    <name>A0A673A8F9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115435842</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A8F9"/>
    <dbReference type="Ensembl" id="ENSSORT00005025604.1">
        <property type="protein sequence ID" value="ENSSORP00005024867.1"/>
        <property type="gene ID" value="ENSSORG00005011969.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A8F9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000450">
        <property type="entry name" value="PR domain zinc finger protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000151">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000638">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF240">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF26.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="201"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="357" mass="41501" checksum="1C9C8E7A3D9A2AA3" modified="2020-06-17" version="1">MEGKLTCDQCGKTFTQASTLRTHLHIHSRERPYSCEECGKAFIRKEVLKIHQRTHSGERPYDCKQCGKTFTHMSALKAHLRIHSGERPYDCKQCGKTFTHMSNLKKHLRIHSGEKPYGCEKCGKTFTEMRSLKAHLRIHSGESPYDCKQCGKTFTHMHHFKTHLRIHSGQRPYHCEQCGKTFTQMSNLQTHLRVHSGERPYYCEQCGKTFTQMSNLKTHLRHIHSGERPYHCEQCEKTFTHVNLFKAHVRIHSGERPYHCCQCGKNYTQMNGLKAHLRIHRRETQRNSLSVTNVNKDSCHTLLSPTINTDVNHRLDQTVPQHQMTTRNAVSEPAAPTSSDKSIKLKNLQIWLHRVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A966</accession>
    <name>A0A673A966_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="59">Patatin-like phospholipase domain-containing protein 2</fullName>
            <ecNumber evidence="7">3.1.1.3</ecNumber>
            <ecNumber evidence="6">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="61">Adipose triglyceride lipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="60">Calcium-independent phospholipase A2-zeta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="65" type="primary">pnpla2</name>
    </gene>
    <organism evidence="65 66">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="65" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="65" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="65" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1,2,3-tri-(10Z)-heptadecenoylglycerol + 9-hydroxy-octadecanoate = 2,3-di-(10Z-heptadecenoyl)-sn-glycerol + 9-(10Z-heptadecenoyloxy)-octadecanoate</text>
            <dbReference type="Rhea" id="RHEA:75019"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:194143"/>
            <dbReference type="ChEBI" id="CHEBI:194145"/>
            <dbReference type="ChEBI" id="CHEBI:228204"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1,2,3-tri-(9Z)-hexadecenoylglycerol + 9-hydroxy-octadecanoate = 2,3-di-(9Z)-hexadecenoyl-sn-glycerol + 9-(9Z-hexadecenoyloxy)-octadecanoate</text>
            <dbReference type="Rhea" id="RHEA:75027"/>
            <dbReference type="ChEBI" id="CHEBI:75841"/>
            <dbReference type="ChEBI" id="CHEBI:75853"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136309"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>1,2,3-tri-(9Z)-hexadecenoylglycerol + H2O = 1,3-di-(9Z)-hexadecenoylglycerol + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:75841"/>
            <dbReference type="ChEBI" id="CHEBI:75849"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:38396"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="56">
            <text>1,2,3-tri-(9Z)-hexadecenoylglycerol + H2O = 2,3-di-(9Z)-hexadecenoyl-sn-glycerol + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:75841"/>
            <dbReference type="ChEBI" id="CHEBI:75853"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="56">
            <dbReference type="Rhea" id="RHEA:38400"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="48">
            <text>1,2,3-tri-(9Z,12Z)-octadecadienoylglycerol + 9-hydroxy-octadecanoate = 2,3-di-(9Z,12Z)-octadecadienoyl-sn-glycerol + 9-(9Z,12Z-octadecadienoyloxy)-octadecanoate</text>
            <dbReference type="Rhea" id="RHEA:75023"/>
            <dbReference type="ChEBI" id="CHEBI:75844"/>
            <dbReference type="ChEBI" id="CHEBI:75854"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:194142"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="49">
            <text>1,2,3-tri-(9Z,12Z)-octadecadienoylglycerol + H2O = 1,3-di-(9Z,12Z)-octadecadienoylglycerol + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38403"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:75844"/>
            <dbReference type="ChEBI" id="CHEBI:75850"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="49">
            <dbReference type="Rhea" id="RHEA:38404"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>1,2,3-tri-(9Z,12Z)-octadecadienoylglycerol + H2O = 2,3-di-(9Z,12Z)-octadecadienoyl-sn-glycerol + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:75844"/>
            <dbReference type="ChEBI" id="CHEBI:75854"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:38408"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1,2,3-tri-(9Z,12Z,15Z)-octadecatrienoylglycerol + H2O = 1,3-di-(9Z,12Z,15Z)-octadecatrienoylglycerol + (9Z,12Z,15Z)-octadecatrienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38411"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32387"/>
            <dbReference type="ChEBI" id="CHEBI:75845"/>
            <dbReference type="ChEBI" id="CHEBI:75852"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:38412"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="54">
            <text>1,2,3-tri-(9Z,12Z,15Z)-octadecatrienoylglycerol + H2O = 2,3-di-(9Z,12Z,15Z)-octadecatrienoyl-sn-glycerol + (9Z,12Z,15Z)-octadecatrienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38415"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32387"/>
            <dbReference type="ChEBI" id="CHEBI:75845"/>
            <dbReference type="ChEBI" id="CHEBI:75855"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="54">
            <dbReference type="Rhea" id="RHEA:38416"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + 9-hydroxy-octadecanoate = 9-(9Z-octadecenoyloxy)-octadecanoate + 2,3-di-(9Z)-octadecenoyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:75011"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
            <dbReference type="ChEBI" id="CHEBI:136282"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = 1,3-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38387"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:38388"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = 2,3-di-(9Z)-octadecenoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38391"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:38392"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + all-trans-retinol = all-trans-retinyl 9Z-octadecenoate + di-(9Z)-octadecenoylglycerol</text>
            <dbReference type="Rhea" id="RHEA:39987"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:70760"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:39988"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + H2O = 1-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38423"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75583"/>
            <dbReference type="ChEBI" id="CHEBI:75867"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:38424"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-3-hexadecanoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75546"/>
            <dbReference type="ChEBI" id="CHEBI:75583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:38644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1,2-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+) = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O</text>
            <dbReference type="Rhea" id="RHEA:38379"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:38381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="47">
            <text>1,3-di-(9Z)-octadecenoyl-2-hexadecanoylglycerol + H2O = 1,3-di-(9Z-octadecenoyl)-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38419"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
            <dbReference type="ChEBI" id="CHEBI:75846"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="47">
            <dbReference type="Rhea" id="RHEA:38420"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>1,3-di-(9Z)-octadecenoyl-2-hexadecanoylglycerol + H2O = 2-hexadecanoyl-3-(9Z)-octadecenoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38431"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75846"/>
            <dbReference type="ChEBI" id="CHEBI:75870"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:38432"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="52">
            <text>1-(9Z-octadecenoyl)-glycerol + 1,2-di-(9Z-octadecenoyl)-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + glycerol</text>
            <dbReference type="Rhea" id="RHEA:38327"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="52">
            <dbReference type="Rhea" id="RHEA:38328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1-(9Z-octadecenoyl)-glycerol + 1,3-di-(9Z-octadecenoyl)-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + glycerol</text>
            <dbReference type="Rhea" id="RHEA:38331"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:38332"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>1-hexadecanoyl-2,3-di-(9Z)-octadecenoyl-sn-glycerol + 9-hydroxy-octadecanoate = 1-hexadecanoyl-3-(9Z)-octadecenoyl-sn-glycerol + 9-(9Z-octadecenoyloxy)-octadecanoate</text>
            <dbReference type="Rhea" id="RHEA:75035"/>
            <dbReference type="ChEBI" id="CHEBI:75847"/>
            <dbReference type="ChEBI" id="CHEBI:75868"/>
            <dbReference type="ChEBI" id="CHEBI:136282"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="55">
            <text>1-hexadecanoyl-2,3-di-(9Z)-octadecenoyl-sn-glycerol + 9-hydroxy-octadecanoate = 9-hexadecanoyloxy-octadecanoate + 2,3-di-(9Z)-octadecenoyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:75015"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
            <dbReference type="ChEBI" id="CHEBI:75847"/>
            <dbReference type="ChEBI" id="CHEBI:83670"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1-hexadecanoyl-2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 1-hexadecanoyl-3-(9Z)-octadecenoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38647"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75847"/>
            <dbReference type="ChEBI" id="CHEBI:75868"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:38648"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1-hexadecanoyl-2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 2,3-di-(9Z)-octadecenoyl-sn-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38427"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
            <dbReference type="ChEBI" id="CHEBI:75847"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:38428"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>2 1-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-glycerol + glycerol</text>
            <dbReference type="Rhea" id="RHEA:38323"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:38324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>2 a 1-acylglycerol = a 1,2-diacylglycerol + glycerol</text>
            <dbReference type="Rhea" id="RHEA:44432"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:35759"/>
            <dbReference type="ChEBI" id="CHEBI:49172"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:44433"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>9-hydroxy-octadecanoate + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 9-(9Z-octadecenoyloxy)-octadecanoate + 2-(9Z-octadecenoyl)-glycerol</text>
            <dbReference type="Rhea" id="RHEA:75031"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
            <dbReference type="ChEBI" id="CHEBI:136282"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="53">
            <text>a 1-acylglycerol + a 1,2-diacylglycerol = a triacylglycerol + glycerol</text>
            <dbReference type="Rhea" id="RHEA:44436"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:35759"/>
            <dbReference type="ChEBI" id="CHEBI:49172"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="53">
            <dbReference type="Rhea" id="RHEA:44437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="51">
            <text>a 1-acylglycerol + a 1,3-diacylglycerol = a triacylglycerol + glycerol</text>
            <dbReference type="Rhea" id="RHEA:44440"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:35759"/>
            <dbReference type="ChEBI" id="CHEBI:47777"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="51">
            <dbReference type="Rhea" id="RHEA:44441"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="57">
            <text>a triacyl-sn-glycerol + H2O = a 1,3-diacyl-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43732"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="ChEBI" id="CHEBI:77272"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="57">
            <dbReference type="Rhea" id="RHEA:43733"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="50">
            <text>a triacyl-sn-glycerol + H2O = a 2,3-diacyl-sn-glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38499"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="ChEBI" id="CHEBI:75524"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="50">
            <dbReference type="Rhea" id="RHEA:38500"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>a triacylglycerol + H2O = a 1,2-diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:49172"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:35668"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a triacylglycerol + H2O = a 1,3-diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38495"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:47777"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:38496"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>a triacylglycerol + all-trans-retinol = an all-trans-retinyl ester + a diacylglycerol</text>
            <dbReference type="Rhea" id="RHEA:44676"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:63410"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:44677"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycerolipid metabolism; triacylglycerol degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="58">Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity. Interacts with SERPINF1; this interaction stimulates the phospholipase A2 activity of PNPLA2. Despite a colocalization in lipid droplets, it probably does not interact with PLIN. Interacts with PLIN5; prevents interaction with ABHD5. Interacts with FAF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="7"/>
    <dbReference type="EC" id="3.1.1.4" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673A966"/>
    <dbReference type="FunCoup" id="A0A673A966">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025978.1">
        <property type="protein sequence ID" value="ENSSORP00005025223.1"/>
        <property type="gene ID" value="ENSSORG00005012133.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A966"/>
    <dbReference type="OrthoDB" id="197155at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050253">
        <property type="term" value="F:retinyl-palmitate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010891">
        <property type="term" value="P:negative regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010898">
        <property type="term" value="P:positive regulation of triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07220">
        <property type="entry name" value="Pat_PNPLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000021">
        <property type="entry name" value="Patatin-like phospholipase domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000003">
        <property type="entry name" value="Patatin-like phospholipase domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033562">
        <property type="entry name" value="PLPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033903">
        <property type="entry name" value="PNPLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406:SF29">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 62" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16 62" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="12" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="19 62" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="66" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PNPLA" evidence="64">
        <location>
            <begin position="10"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="63">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="62">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="62">
        <location>
            <begin position="45"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="62">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="62">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="62">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00050132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00050160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00050235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00050245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00050373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00050418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00050444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00050453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00050520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00050589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00050685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00050827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00051063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00051078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00051283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00051457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00051536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00052125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00052222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00052229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00052309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="ARBA" id="ARBA00052366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="48">
        <source>
            <dbReference type="ARBA" id="ARBA00052389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="49">
        <source>
            <dbReference type="ARBA" id="ARBA00052519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="50">
        <source>
            <dbReference type="ARBA" id="ARBA00052610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="51">
        <source>
            <dbReference type="ARBA" id="ARBA00052649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="52">
        <source>
            <dbReference type="ARBA" id="ARBA00052658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="53">
        <source>
            <dbReference type="ARBA" id="ARBA00052713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="54">
        <source>
            <dbReference type="ARBA" id="ARBA00052815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="55">
        <source>
            <dbReference type="ARBA" id="ARBA00052816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="56">
        <source>
            <dbReference type="ARBA" id="ARBA00052855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="57">
        <source>
            <dbReference type="ARBA" id="ARBA00052907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="58">
        <source>
            <dbReference type="ARBA" id="ARBA00065722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="59">
        <source>
            <dbReference type="ARBA" id="ARBA00072609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="60">
        <source>
            <dbReference type="ARBA" id="ARBA00076067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="61">
        <source>
            <dbReference type="ARBA" id="ARBA00076473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="62">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="63">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="64">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="65">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="66">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="504" mass="56099" checksum="1D586066B2D58271" modified="2020-06-17" version="1">MFPLDTPWNISFAGCGFLGIYHVGVASCLQEQAPFLVHNARHIYGASAGALTATALVTGVCLGEAGASIIDVAKEARKRFLGPMHPSFNLVKIVCRMLRRTLPADCHHQATGRLGISLTRVTDGENVLVSHFNTKEELVQACVCSAYIPVYCGLIPPSLRGVRYVDGGISDNLPQYELKNTITVSPFSGESDICPRDTSTNLHELRFTNTSIQFTLTNLYRVSRALFPPDPVVMKTMCKQGYKDALHFLKRNGLLNFNGPHRDRLLIAKGEENEDDDRSDGEDGEPDVEERAQMVQSSSFIEEHFMENLPPALHKALVEACRERRSLTQSLSNLLPVRMASAMMLPYTLPLESAMSLTLRLLEWLPYVQEDVGWIREQVLKILQYVLRQVSRSITQHVSARFSCQLELYHHQSLPRQMSSSSLFPTWVNESSSSVIDVFMRLEQYKRQLLSGVLCINMDLQGSFKTGPLSADKSPSSILSSEGLTIHRDHSDIQPASAEVTSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A9U4</accession>
    <name>A0A673A9U4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RUN domain-containing protein 3B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rundc3b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the RUNDC3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030014390.1">
        <property type="nucleotide sequence ID" value="XM_030158530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A9U4"/>
    <dbReference type="FunCoup" id="A0A673A9U4">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026820.1">
        <property type="protein sequence ID" value="ENSSORP00005026051.1"/>
        <property type="gene ID" value="ENSSORG00005012512.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435905"/>
    <dbReference type="CTD" id="154661"/>
    <dbReference type="InParanoid" id="A0A673A9U4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="CDD" id="cd17700">
        <property type="entry name" value="RUN_RUNDC3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047339">
        <property type="entry name" value="RUN_RUNDC3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047340">
        <property type="entry name" value="RUNDC3A_B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46251">
        <property type="entry name" value="RUN DOMAIN-CONTAINING 3 PROTEIN RUNDC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46251:SF1">
        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="5">
        <location>
            <begin position="48"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="449" mass="50161" checksum="9B3D82EF1A1A88B4" modified="2020-06-17" version="1">MASLGVGMHLIRKRVAGRSAAVERRNLLTVCRFSVKTLLDRSCFETIDDSSPEFINFVSILEHILSHRLKGQTTWFGYESPRSFWDYIKAACSKVPHNCIRSIESMENVRSSRAKGRAWVRVVLMEKRLSEYISSALRDFKTTRRFFEDGAIMLGEEAGLLADTLIGLNTIDFSFCLKGEGLDGSCPAVIDYTPYLKFVQNADSISSDEEEMRTLGSSGSESSTPDKTATAASIFTEQSNLVSKCKRFEQKYRMALEQKGYLEELVRLREAQLSEAVSHNKALQQSLADTHLSHTLEKEQLEYIILELQDQLTVLKNNDLRSRQELTTHLTNQWPSPDALDANAVALDTLLYRKSTGQWEEKSFHSLEQLSADMSLSQTSPEPLHTQSLEARSAGTQWLQRGKEETPSLRGLCGSLTSVASYKSLASLKSSEYLASPATEISSPGVTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABI9</accession>
    <name>A0A673ABI9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ABI9"/>
    <dbReference type="Ensembl" id="ENSSORT00005026685.1">
        <property type="protein sequence ID" value="ENSSORP00005025917.1"/>
        <property type="gene ID" value="ENSSORG00005012442.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABI9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06481">
        <property type="entry name" value="ACD_HspB9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF2">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="66"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="203" mass="22911" checksum="33952B849D598DB0" modified="2020-06-17" version="1">MLCSHGWRSGLSPLLDVHWPVLSLRPQVTPLLYQQDLLLTNLQQLRSRLELMEPLQHKTPEQTEPFQTSVALQPLSYQLTTDGQRFGLSLDTGGFSPEELSVRQVGRKLRVSGKTEKKQEDAKGCSSYRRQEFRQELDLPEGVKPETLSCYLGPDGKLHIQADKAPCVEEAEREVPINRSSEEKTQTEGGSSTQKTKALSYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABN0</accession>
    <name>A0A673ABN0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein PET100 homolog, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115424915</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029998172.1">
        <property type="nucleotide sequence ID" value="XM_030142312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ABN0"/>
    <dbReference type="FunCoup" id="A0A673ABN0">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026720.1">
        <property type="protein sequence ID" value="ENSSORP00005025952.1"/>
        <property type="gene ID" value="ENSSORG00005012461.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424915"/>
    <dbReference type="CTD" id="100131801"/>
    <dbReference type="InParanoid" id="A0A673ABN0"/>
    <dbReference type="OrthoDB" id="18175at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025494598" evidence="9">
        <location>
            <begin position="28"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="78" mass="9713" checksum="2F5C6D314DCD9AFC" modified="2020-06-17" version="1">MGVKLEVFRMMLYLSFPVAMFWVSNQAEYFEEYIVKRKREIFPPNEEYQRKELEDFKEKMHVRKEQRLLKQMSVESEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACE6</accession>
    <name>A0A673ACE6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transmembrane protein 235b</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ACE6"/>
    <dbReference type="FunCoup" id="A0A673ACE6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026963.1">
        <property type="protein sequence ID" value="ENSSORP00005026192.1"/>
        <property type="gene ID" value="ENSSORG00005012573.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ACE6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039951">
        <property type="entry name" value="TMEM114/TMEM235"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20516">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 114/235 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20516:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="218" mass="23795" checksum="1D0DDE42E2B27FA3" modified="2020-06-17" version="1">MMRITFGFLVISAGVCGIMSFAFLATSLGTEYWYIIETNPVNMSDFEDMSSHSGLWTINEGRGRHRTPRMLHNPSVFVCALYAELHSAIVVVLPLSLVLLLLGGICGLVSSLAQSSFLLTGTASYFFICSKLRGLLTLCGVSLYIVYCCQALAETERLVGPEGLAYIHMSFGWSLGLAWLSYGLELLTGVLLLMAARMVKLSQSSPSAAGSFYIKYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACW1</accession>
    <name>A0A673ACW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Synaptophysin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">sypa</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homohexamer or homotetramer. Interacts with SRCIN1. Interacts with VAMP2; the interaction is inhibited by interaction of VAPM2 with SEPT8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synapse</location>
            <location evidence="15">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ACW1"/>
    <dbReference type="FunCoup" id="A0A673ACW1">
        <property type="interactions" value="312"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027891.1">
        <property type="protein sequence ID" value="ENSSORP00005027106.1"/>
        <property type="gene ID" value="ENSSORG00005012961.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ACW1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF10">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00220">
        <property type="entry name" value="SYNAPTOPHYSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 18 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="6 18 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="21">
        <location>
            <begin position="14"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="294" mass="32999" checksum="8C9A22DE84F95994" modified="2020-06-17" version="1">MDVDILVAQGQFRILKVPLGFIKVLQWFFAIFAFSTCGSYSGMFRMSVECKNRTESNLAIEVDFEYPFRLHQVYFDAPTCKDRSTDRYFLVGDYSSSAEFFVTIGVFAFLYSTAALSIYIFFFEKYKENNKGPLVDLGVTGVFAFMWLVSSAAWAKGLSDVKAATDPDNVITMIDACDVEGNRCREVHDAVMSGLNTSVAFGFINLVLWVGNLWFVFKETGIIAPFMRAPPPQGKPAAPDAYGQQGGYEQDPYASNQGGYQPDYSQQGYNQVTHTHLGGQFIFLWGHMRNFDSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEM7</accession>
    <name>A0A673AEM7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Iron-responsive element-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ireb2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AEM7"/>
    <dbReference type="FunCoup" id="A0A673AEM7">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028878.1">
        <property type="protein sequence ID" value="ENSSORP00005028075.1"/>
        <property type="gene ID" value="ENSSORG00005013446.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AEM7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030350">
        <property type="term" value="F:iron-responsive element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
        <property type="entry name" value="AcnA_IRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000005">
        <property type="entry name" value="cytoplasmic aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000011">
        <property type="entry name" value="Iron-responsive element binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000012">
        <property type="entry name" value="Iron-responsive element binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000005">
        <property type="entry name" value="Iron-responsive element-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="78"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="213"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="16">
        <location>
            <begin position="785"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="149"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="980" mass="107160" checksum="52691DFEBF061A25" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGF4</accession>
    <name>A0A673AGF4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Disintegrin and metalloproteinase domain-containing protein 33-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">adam19a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AGF4"/>
    <dbReference type="Ensembl" id="ENSSORT00005028256.1">
        <property type="protein sequence ID" value="ENSSORP00005027467.1"/>
        <property type="gene ID" value="ENSSORG00005013119.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGF4"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF38">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="712"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="13">
        <location>
            <begin position="220"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="12">
        <location>
            <begin position="427"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="661"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="785"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="838"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="883"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="961" mass="104356" checksum="CC34C30C82B415CD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHH7</accession>
    <name>A0A673AHH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">paox</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030011399.1">
        <property type="nucleotide sequence ID" value="XM_030155539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AHH7"/>
    <dbReference type="FunCoup" id="A0A673AHH7">
        <property type="interactions" value="815"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028588.1">
        <property type="protein sequence ID" value="ENSSORP00005027792.1"/>
        <property type="gene ID" value="ENSSORG00005013296.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433949"/>
    <dbReference type="CTD" id="196743"/>
    <dbReference type="InParanoid" id="A0A673AHH7"/>
    <dbReference type="OrthoDB" id="2019015at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046203">
        <property type="term" value="P:spermidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF405">
        <property type="entry name" value="PEROXISOMAL N(1)-ACETYL-SPERMINE_SPERMIDINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="502" mass="55891" checksum="C057EF185B18B3DD" modified="2020-06-17" version="1">MGGAEKALAMRQNAKIVIIGCGISGIAAAQRLFNAGFHNVRIIEATARSGGRIKTGSLGDKIIEIGANWIHGPSEENPVFCLARQYGLLEPEALTPENQAMDIEGHPPWVPNFFTSSGRKLNAEDMYPAQQIFMELLDESSEVHSQGGEPFPSVGDFIRSKAAEKCQDTDAATRSLQLCVISNMLKVECCVNGTHSMDKVGLGAFGLYKTLPGLDCTFPKGYEGLIKNLMSELPSGLVTYNRPVRCVHWNNEEKQTPVIVECDNGERTAADHVIVTVSLGYLKKHHSTLFHPRLPLHKLHSVQRLGFGTNNKIFVEFDSPWWDADCEVIYFLWEDEVDMVDQIPDVQRSWIKKLFGFTVLKPTERYGHVLCGWIAGHESEYMETLSEQEVTHAVTKLVRQFTGNPIITPTRVLRSQWFHDPWTCGSYSYPGKGCSAQDLDNLREPMPTKGSQPLQVLFAGEATHPCFYSTVHGAVLSGWREADRLISHYSSISPSKHTNSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIJ5</accession>
    <name>A0A673AIJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Serine/threonine-protein kinase STK11</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">stk11</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673AIJ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005029909.1">
        <property type="protein sequence ID" value="ENSSORP00005029089.1"/>
        <property type="gene ID" value="ENSSORG00005013883.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AIJ5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14119">
        <property type="entry name" value="STKc_LKB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000245">
        <property type="entry name" value="serine/threonine-protein kinase STK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000235">
        <property type="entry name" value="serine/threonine-protein kinase STK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039154">
        <property type="entry name" value="LKB1_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF94">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="16 24 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 24 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 25" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="47"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="361"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="413"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00068788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="423" mass="47662" checksum="404D0764758911F8" modified="2020-06-17" version="1">MSTGELHHLDYLNENELMEMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEMLDSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLQHKNVIQLVDYCVCGMQEMLDSVPEKRFPVFQAHGYFCQLLDGLEYLHSQGIVHKDIKPGNLLLTTDGALKISDLGVAEALHPFAEDDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTSLYPFEGDNIYKLFENIGKGDYTIPEECGPLLSDLLRGMLEYDPAKRFSIQNIRQHNWVRKKHPPSEPPVPIPASAESRDPWRSMTVVPYLEDLHGYTEEDDDELYDGEDEIIYTQDFTVPGQVAEEDRDQANADHSPAVAKPVCVNGTEGVSLNSKAKAERRSSSSSNPSRKGVSTASKIRKLSTCKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKC6</accession>
    <name>A0A673AKC6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ras homolog family member F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rhof</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030005111.1">
        <property type="nucleotide sequence ID" value="XM_030149251.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030005112.1">
        <property type="nucleotide sequence ID" value="XM_030149252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AKC6"/>
    <dbReference type="Ensembl" id="ENSSORT00005029613.1">
        <property type="protein sequence ID" value="ENSSORP00005028797.1"/>
        <property type="gene ID" value="ENSSORG00005013760.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429635"/>
    <dbReference type="CTD" id="54509"/>
    <dbReference type="InParanoid" id="A0A673AKC6"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04132">
        <property type="entry name" value="Rho4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000676">
        <property type="entry name" value="Ras homolog family member F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23757" checksum="A470CDA5249C88DE" modified="2020-06-17" version="1">MTQNSSRSGTGTTRKGSELKIVIVGDGGCGKTSLLMVYAKGDFPEKYAPSVFEKYVTTISLGGKEIKLNLYDTAGQDDYDRLRPLSYQEANLVLVCFDVTNPTSYDNVLIKWFPEVKHFCRDTPVVLIGCKTDLRKDSECSRKLKNMNQTPITSIQGEDARQQMNAELYLECSAKYQENVEHIFREATKTALAFIKKQRNSRKIKKCVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALF6</accession>
    <name>A0A673ALF6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Collapsin response mediator protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">crmp1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ALF6"/>
    <dbReference type="Ensembl" id="ENSSORT00005030927.1">
        <property type="protein sequence ID" value="ENSSORP00005030084.1"/>
        <property type="gene ID" value="ENSSORG00005014363.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALF6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016812">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.40.10:FF:000021">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000174">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF54">
        <property type="entry name" value="DIHYDROPYRIMIDINASE-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="67"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="511"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="571" mass="61971" checksum="CE4E53400E9890F2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARC5</accession>
    <name>A0A673ARC5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytochrome c oxidase subunit 5B, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome c oxidase polypeptide Vb</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">zgc:86599</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ARC5"/>
    <dbReference type="Ensembl" id="ENSSORT00005032936.1">
        <property type="protein sequence ID" value="ENSSORP00005032051.1"/>
        <property type="gene ID" value="ENSSORG00005015230.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARC5"/>
    <dbReference type="OrthoDB" id="10249250at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00924">
        <property type="entry name" value="Cyt_c_Oxidase_Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.11.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 5B, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002124">
        <property type="entry name" value="Cyt_c_oxidase_su5b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122:SF20">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01215">
        <property type="entry name" value="COX5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00848">
        <property type="entry name" value="COX5B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51359">
        <property type="entry name" value="COX5B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="12">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="12">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="12">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="126" mass="13883" checksum="D1649CF7ACB55C25" modified="2020-06-17" version="1">MAARLLLRSAYRAATTCRATRVPVLTRGMAAGGIPSDEEQATGMEKIIMRAMKDGTDPYNMLKPKEYAGSKADPHLVPSITNKRIVGCVCEEDNTAVVWFWLHEGEAQRCPSCGSHYKLVAHELPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AT15</accession>
    <name>A0A673AT15_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LIM domain binding 3b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ldb3b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AT15"/>
    <dbReference type="Ensembl" id="ENSSORT00005033624.1">
        <property type="protein sequence ID" value="ENSSORP00005032726.1"/>
        <property type="gene ID" value="ENSSORG00005015536.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AT15"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06753">
        <property type="entry name" value="PDZ_PDLIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000019">
        <property type="entry name" value="LIM domain binding 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF9">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="288" mass="30951" checksum="71C7105E3BBD6A71" modified="2020-06-17" version="1">MSGAYTVTLNGPAPWGFRLQGGKDFNMPLTISRITPGSKAASGSLAQGDIISAIDGVSTDGMTHLEAQNKIKLATHKLALSMLKSRRPAPVPTATPRMDSPMPVIPHQKVIANTAANVEYTPSFNPIALKDSALSTHKPIEVRGPGGKATIVHAQYNTPISMYSQDAIMDAIAGQSQAKGEEAGSPLAGALPVKEPVVDCASPVYQAVIRPGETPQDMSEWARRAANLQSKSFRILAHITGTEYLQDPDEEALQRSREKFESEVKGPRFAKLKNWHHGLSAQILNVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AT92</accession>
    <name>A0A673AT92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CARD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AT92"/>
    <dbReference type="Ensembl" id="ENSSORT00005033791.1">
        <property type="protein sequence ID" value="ENSSORP00005032890.1"/>
        <property type="gene ID" value="ENSSORG00005015603.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AT92"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="85" mass="9564" checksum="E6A0CF224DC1148F" modified="2020-06-17" version="1">MSASLTFGFIHGVSEPNLKSLLDKLLEKGVVSDAEMDEVKGMQTRADKARFVIDTVRRKGKKASSEMIEFLRELDPYLCDDLDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATV1</accession>
    <name>A0A673ATV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gata3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030005991.1">
        <property type="nucleotide sequence ID" value="XM_030150131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ATV1"/>
    <dbReference type="FunCoup" id="A0A673ATV1">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033994.1">
        <property type="protein sequence ID" value="ENSSORP00005033090.1"/>
        <property type="gene ID" value="ENSSORG00005015692.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430214"/>
    <dbReference type="CTD" id="2625"/>
    <dbReference type="InParanoid" id="A0A673ATV1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002520">
        <property type="term" value="P:immune system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030856">
        <property type="term" value="P:regulation of epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000001">
        <property type="entry name" value="GATA transcription factor (GATAd)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000032">
        <property type="entry name" value="Transcription factor GATA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016374">
        <property type="entry name" value="TF_GATA-2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF106">
        <property type="entry name" value="TRANS-ACTING T-CELL-SPECIFIC TRANSCRIPTION FACTOR GATA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003027">
        <property type="entry name" value="TF_GATA-1/2/3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="255"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="309"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="GATA-type 1" evidence="11">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="GATA-type 2" evidence="11">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="126"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="354"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="416"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR003027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="442" mass="47910" checksum="1FCD23556E70245B" modified="2020-06-17" version="1">MEVSADQPRWMSHHPAVLNEQHPGSHHPGLGHSYMDPSQYPLADDVDVLFNIDGQGNHVSPYYGNSVRAVQRYPPPPHSSQVCRPSLLHGSLPWLEGSKGIAPHHSTSPWNLSPFPKNPLHHGSPASLSVYPPASSSSLSTGHSSPHLFTFPPTPPKDVSPDPGMSTPGSSSSGRQEDKECIKYQVTLAESMKLESAHSRSVGSIGAGSSSAHHPIATYPPYVPEYGPGLFPPSSLIGGSSSSYGSKTRPKTRSCSEGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYFKLHNINRPLTMKKEGIQTRNRKMSSKSKKSKKSQDNMDDFSKSLMDKSSSFSPAALSRHMSSFPPFSHSSHMLTTPTPMHPSSSLQFAPHHSSMVTAMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWF3</accession>
    <name>A0A673AWF3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sorting nexin 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AWF3"/>
    <dbReference type="Ensembl" id="ENSSORT00005033536.1">
        <property type="protein sequence ID" value="ENSSORP00005032638.1"/>
        <property type="gene ID" value="ENSSORG00005015492.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AWF3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45850">
        <property type="entry name" value="SORTING NEXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45850:SF4">
        <property type="entry name" value="SORTING NEXIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10948" checksum="68DC0018FB78BB1D" modified="2020-06-17" version="1">MMEGLDDGPDFLSEEDRGVSPRAVNVDLQTDATLQVDISDALSERDKVKFTVHTKSTLPNFKQNEFSVVRQHEEFIWLHDSFVENEEYAGYIVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZC0</accession>
    <name>A0A673AZC0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nuclear factor of activated T cells 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nfatc4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AZC0"/>
    <dbReference type="Ensembl" id="ENSSORT00005035929.1">
        <property type="protein sequence ID" value="ENSSORP00005035000.1"/>
        <property type="gene ID" value="ENSSORG00005016503.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZC0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF11">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="366"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="RHD" evidence="11">
        <location>
            <begin position="27"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="406" mass="45672" checksum="810050E17CADC364" modified="2020-06-17" version="1">MDYLSVPPALAWGRTRASAHSPLFRSNALPPLDWPLPSQFDQYELRIEVQPRPHHRAHYETEGSRGAVKATPTGHPVVKLCGYTERKPLSLQVFVGTADDRSIRPHPFYQIHRVTGKMVGTASHESVQAGTKLLDIPLTPENNMTALIDCAGILKLRNSDIELRKGETDVGRKNTRVRLVFRTHLHLAPPVAPPGRVLALQVASLPIECSQRSAQELPVIESVSLTSCSVEGGEELLLSGTNFLPISRVLFMERGTDGKLQWEEEAHVDRDNSSECLLCVRVPAYSDLSVSRPVSISLYVSNGKRKRSSTHSFKYLPSECLILLCSPLIFYLHPPLAEVTLCWSVKYYRTCFKDNLIVLSLLLPKYFTFFTSILIVSLLLLRYFSSFIIFYCFFCLSVLRNCNIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZJ9</accession>
    <name>A0A673AZJ9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 42b</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AZJ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005036255.1">
        <property type="protein sequence ID" value="ENSSORP00005035306.1"/>
        <property type="gene ID" value="ENSSORG00005016676.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZJ9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039632">
        <property type="entry name" value="TMEM42"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31965">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31965:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="106" mass="11560" checksum="4DFFA77D4EA3006C" modified="2020-06-17" version="1">MRGGAANECLHIPLRLLCGGLLFTCNAVMWTFLAKALRYSSSSTRTTVTTTASNFISSAFLGQLIFGEAQITLWWVGISLTFSGLLVLQRVSPQDTQQNTGTKKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZP9</accession>
    <name>A0A673AZP9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thymus-specific serine protease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AZP9"/>
    <dbReference type="Ensembl" id="ENSSORT00005034666.1">
        <property type="protein sequence ID" value="ENSSORP00005033753.1"/>
        <property type="gene ID" value="ENSSORG00005015983.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZP9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF11">
        <property type="entry name" value="THYMUS-SPECIFIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24468" checksum="E9F24E533BE2A8DD" modified="2020-06-17" version="1">AGQKYYQQIKDPCLDVSHKKTVKHLMNTSISSGRMSERQWIYQTCTEFGFYQTCEDAACPFSGMLTLQADTELCPVLFGISQHSLPRRIAFTNSYYGEDNPHTHRVLYVNGGVDPWKALSVMEDRTECGDESQLIFIKDTAHCADMMHKRNTDRSSLRNARKVCQTSQTRYLSICLCISRTSGIQSSTLLTIFKVTHQTASILTHQCYISLFLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZV6</accession>
    <name>A0A673AZV6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pleurocidin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AZV6"/>
    <dbReference type="Ensembl" id="ENSSORT00005036130.1">
        <property type="protein sequence ID" value="ENSSORP00005035195.1"/>
        <property type="gene ID" value="ENSSORG00005016604.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZV6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012515">
        <property type="entry name" value="Antimicrobial12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08107">
        <property type="entry name" value="Antimicrobial12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025401056" evidence="6">
        <location>
            <begin position="23"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="78" mass="8699" checksum="D6E647FE01845102" modified="2020-06-17" version="1">MKGVMIFLILSLVVLMAEPGECGWGKLVKRMDLMMAKEAMEARLTAADSENAYEEGRPEDGEFSISSVALHFLIMNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1Z3</accession>
    <name>A0A673B1Z3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Mitochondrial basic amino acids transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Carnitine/acylcarnitine translocase-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Mitochondrial carnitine/acylcarnitine carrier protein CACL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Mitochondrial ornithine transporter 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Solute carrier family 25 member 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">slc25a29</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-histidine(out) = L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:72807"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-homoarginine(in) + L-arginine(out) = L-homoarginine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:72799"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:143006"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysine(out) + L-arginine(in) = L-lysine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:70827"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-ornithine(in) + L-arginine(out) = L-ornithine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:34991"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(omega)-methyl-L-arginine(in) + L-arginine(out) = N(omega)-methyl-L-arginine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:72803"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 26">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B1Z3"/>
    <dbReference type="Ensembl" id="ENSSORT00005035585.1">
        <property type="protein sequence ID" value="ENSSORP00005034662.1"/>
        <property type="gene ID" value="ENSSORG00005016337.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B1Z3"/>
    <dbReference type="OrthoDB" id="193856at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005289">
        <property type="term" value="F:high-affinity L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000037">
        <property type="entry name" value="Solute carrier family 25 member 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF61">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="10 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 25 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 26" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="88"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="192"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00079387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00080567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="307" mass="33562" checksum="AE28B19D71A1F5C7" modified="2020-06-17" version="1">MDFLAGCIGGAAGVLVGHPFDTVKVRLQVQSVDKPLYRGTFHCFQSIIRQESVFGLYKGIGSPMMGLTFINAIVFGVQGNTMRLLGTDTPMNQFLAGAAAGAIQCVICCPMELAKTRMQMQGTGEKKSSRKMYKNSLDCLARIYNREGLWGVNRGMVTTLIRETPGFGVYFLAYDVLTRSLGCEPDDRYMIPKLLFAGGMAGIASWLSTYPVDVIKSRLQADGVGGVHQYSSIADCVRQSVRREGYMVFTRGLTSTLLRAFPVNAATFATVTLVLMYARGIEEPKDCELTPPSHHAQLQQQAQPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B248</accession>
    <name>A0A673B248_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Betaine--homocysteine S-methyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">bhmt</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the regulation of homocysteine metabolism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B248"/>
    <dbReference type="Ensembl" id="ENSSORT00005037252.1">
        <property type="protein sequence ID" value="ENSSORP00005036291.1"/>
        <property type="gene ID" value="ENSSORG00005017060.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B248"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00083"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047150">
        <property type="term" value="F:betaine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071267">
        <property type="term" value="P:L-methionine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.330:FF:000003">
        <property type="entry name" value="Betaine--homocysteine S-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051524">
        <property type="entry name" value="BHMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="BHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46120">
        <property type="entry name" value="BETAINE--HOMOCYSTEINE S-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46120:SF1">
        <property type="entry name" value="HCY-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="9">
        <location>
            <begin position="8"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037505-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="399" mass="44149" checksum="09FB19B945D36620" modified="2020-06-17" version="1">MAPAGTKRGILERLDAGEVVIGDGGFVFALEKRGYVKAGPWTPEAAAENPEAVRQLHREFLRAGSNVMQTFTFYASDDKLENRGHTQRFTLLTHLISACDLAREVANEGDALVAGGVSQTPSYLSCKSEDEVKAIFKKQIDVFVKKDVDFLIAEYFEHVEEAEWAVQVLKTTGKPVAASLCIGPEGDLNGVSPGDCAVRLVKAGAQIVGINCHFDPETCVKTVKMMKMGVEKAGLKAHYMCQPLAYHTPDCNRQGFIDLPEFPFGLEPRILTRWDMQKYARDAYNVGIRFIGGCCGFEPYHIRALAEELATERGFLPPGSEKHGLWGSGLEMHTKPWVRARARRDYWETLKPASGRPFCPSMAAPDGWGITKGHADLMQQREATSQEQLKALFDKATKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3V6</accession>
    <name>A0A673B3V6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115432039</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B3V6"/>
    <dbReference type="FunCoup" id="A0A673B3V6">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037874.1">
        <property type="protein sequence ID" value="ENSSORP00005036906.1"/>
        <property type="gene ID" value="ENSSORG00005017355.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3V6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000018">
        <property type="entry name" value="Transient receptor potential cation channel subfamily V member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024862">
        <property type="entry name" value="TRPV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008347">
        <property type="entry name" value="TrpV1-4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00870">
        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582:SF5">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY V MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01768">
        <property type="entry name" value="TRPVRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 15" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="547"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="328"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="592" mass="68144" checksum="D9B951323ABB83A8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B468</accession>
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        <submittedName>
            <fullName evidence="6">DENN domain containing 10</fullName>
        </submittedName>
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        <name evidence="6" type="primary">LOC115434084</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Late endosome</location>
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        <text evidence="2">Belongs to the DENND10 family.</text>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50211"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037021.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="351" mass="40055" checksum="F9841D9B63A57008" modified="2020-06-17" version="1">MDTKFVYKLRELTVFYNYKPLELILGHVSRVTQILHMVEKDVNGDTLWVWCYPSVDSQLRQVLLSKCCLTQDGRDFHTFVFGQFCRTWYYITTVEVQEPTALSKVQCDSFSIVVTAKDFNPEKYAALSRILCRMYVKHGSPVKMMEAYITVLTKGICQSDENGSFLIKDYDVRKAYLAGSVKDVVSQFGMETIILYTALMLKKRIVVHHPRIEALLEFTRVLPALTWHRKDWSILHPYVHLTETELEDLSRCPGYVAGFVDPEVSNRSDLFDVYVNLPDSVITVSQSAKEAMAMGKLHKDIGHLIVQSAEDTDRSDSQVIKDISVKTKEILSNLVSLANECDDSKLTLDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4F8</accession>
    <name>A0A673B4F8_9TELE</name>
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        <submittedName>
            <fullName evidence="5">Hematopoietic SH2 domain-containing protein homolog</fullName>
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        <name evidence="5" type="primary">sh2d7</name>
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    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="119"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="447" mass="49856" checksum="DD8AB96EA89BE23D" modified="2020-06-17" version="1">MEKKSGVELQTEPSEGGLKELVLRWFTETQAPFMMHNGNFPDWFQGFTTRKDAEDLLRDKALGCFLIRLSDKTIGYILSYKGHDRCRHFVITQNQEGFFIISGDCQTYGSLVELIEHYKVSPIQPFGEYLTSNSYEEDSSDLYDVVNYEAKGKSGVSVQALRTLWDQRGDQQSELTTNQKLQQPSVCGAVQPPALPLKAKSRRLTGTVSVDAMTLTQGVPPLPKRGIPLGFSLGGSLPDTTLHPIETPAEPNRAKTPPENPKSEPVCNRAEDSAKPSDTSRAVDTPQITYSELTKVESRSRSLPRLDNNMEEEAYSNRITTPASHSPSALKKVTCHTYSLQEPRDGLRLSSSDEMLRSNPLYHSSGGAGGRSAQQGDNMYAEVPQIPTPTTVPETTYEKIPEPGSTNPTNTYETLDDMKTQKSKSQKSTWGKNNLRWKIFFPEHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5D7</accession>
    <name>A0A673B5D7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Empty spiracles homeobox 2</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
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            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMX homeobox family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A673B5D7"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Empty spiracles homeobox 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="HD_metazoa"/>
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    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339">
        <property type="entry name" value="HOMEOBOX PROTEIN EMX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339:SF25">
        <property type="entry name" value="HOMEOBOX PROTEIN EMX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="115"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="117"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="175"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="215" mass="24835" checksum="62C9AD2127F5326F" modified="2020-06-17" version="1">YYVHNLKQEPSWPAALSYANSGQMNPFLNGFHSGGRGVYSNPDLVFAEAVSHPPNSAVPVHPVARRTPHPLFASQQRDPSTFYPWLLHRYRYLGHRFQGNETSPESFLLHNALARKPKRIRTAFSPSQLLRLEHAFEKNHYVVGAERKQLAHSLSLTETQVKVWFQNRRTKFKRQKLEEEGSESQQKKKGSHHINRWRLATKQSSPEEIDVTSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9D9</accession>
    <name>A0A673B9D9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phosphatidylinositol 4-kinase type 2</fullName>
            <ecNumber evidence="19">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">pi4k2b</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:19878"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="19"/>
    <dbReference type="RefSeq" id="XP_030001203.1">
        <property type="nucleotide sequence ID" value="XM_030145343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B9D9"/>
    <dbReference type="FunCoup" id="A0A673B9D9">
        <property type="interactions" value="1456"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039953.1">
        <property type="protein sequence ID" value="ENSSORP00005038951.1"/>
        <property type="gene ID" value="ENSSORG00005017288.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427007"/>
    <dbReference type="CTD" id="55300"/>
    <dbReference type="InParanoid" id="A0A673B9D9"/>
    <dbReference type="OrthoDB" id="3349449at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF6">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="21">
        <location>
            <begin position="147"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="88"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="508" mass="56990" checksum="83580A8DED6E1E2B" modified="2020-06-17" version="1">MMSECDPTETEAEPVLDTVDTAVAPPECNFHSADPQNVLFDTNRIGLGLAANPGVAVRISGSCESVLTELETDGSGEEALLLPCLAGSSSSPRAARDKRSRRNRHSSSSDKDTLASPGTHGGDFNHFPDDPEFADIIQRAEHAIENGVFPERISQGSSGSYFVKDPKGKIIGVFKPKSEEPYGHLNPKWTKYFHKLCCPCCFGRGCLLPNQGYLSEAAASLVDSKLGLGVVPKTKVVYLASETFHYSAIDRAKSRGKKYALEKVPKVGRRFHRVGLPPKVGSFQLFVEGYREADYWLRRFEAEPLPENIRKQLQSQFERLVVLDYVIRNTDRGNDNWLIKYEKSGEEGGEQKDAEWPENSLDSCIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVAFSQETRDLVLSRLSDMNFVQDLCEDLYEMFKTDKGFDKTMFERQMSVMRGQVLNLTQALKDGKSPIQLVQMPRVVVERSRSGGQGRVVTLGNAFTQTFHCKRPFFSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BD95</accession>
    <name>A0A673BD95_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">galnt7</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Golgi apparatus membrane</location>
            <topology evidence="2 18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_029989068.1">
        <property type="nucleotide sequence ID" value="XM_030133208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BD95"/>
    <dbReference type="FunCoup" id="A0A673BD95">
        <property type="interactions" value="1029"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041518.1">
        <property type="protein sequence ID" value="ENSSORP00005040476.1"/>
        <property type="gene ID" value="ENSSORG00005018348.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418663"/>
    <dbReference type="CTD" id="51809"/>
    <dbReference type="InParanoid" id="A0A673BD95"/>
    <dbReference type="OrthoDB" id="6072411at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23437">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000053">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF68">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 18" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="20">
        <location>
            <begin position="523"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="646" mass="73715" checksum="B681A22CF7931DCF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BG14</accession>
    <name>A0A673BG14_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Peptidase M60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115431224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCAF family.</text>
    </comment>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041773.1">
        <property type="protein sequence ID" value="ENSSORP00005040720.1"/>
        <property type="gene ID" value="ENSSORG00005019012.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BG14"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090314">
        <property type="term" value="P:positive regulation of protein targeting to membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TRPM8 channel-associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.80">
        <property type="entry name" value="Peptidase M60, enhancin-like domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.30">
        <property type="entry name" value="Peptidase M60, enhancin-like domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Class_I_gatase-like"/>
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    </dbReference>
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        <property type="entry name" value="Pep_M60_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031161">
        <property type="entry name" value="Peptidase_M60_dom"/>
    </dbReference>
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        <property type="entry name" value="TCAF"/>
    </dbReference>
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        <property type="entry name" value="EXPERIMENTAL AUTOIMMUNE PROSTATITIS ANTIGEN 2-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15730:SF5">
        <property type="entry name" value="SI:CH211-210B2.2-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF17291">
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    </dbReference>
    <dbReference type="Pfam" id="PF13402">
        <property type="entry name" value="Peptidase_M60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01276">
        <property type="entry name" value="M60-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51723">
        <property type="entry name" value="PEPTIDASE_M60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M60" evidence="2">
        <location>
            <begin position="510"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="881" mass="98009" checksum="8075B93B0C557301" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BG17</accession>
    <name>A0A673BG17_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ascl1a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BG17"/>
    <dbReference type="FunCoup" id="A0A673BG17">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040296.1">
        <property type="protein sequence ID" value="ENSSORP00005039288.1"/>
        <property type="gene ID" value="ENSSORG00005018345.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BG17"/>
    <dbReference type="OrthoDB" id="5976910at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021575">
        <property type="term" value="P:hindbrain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19742">
        <property type="entry name" value="bHLH_TS_ASCL1_Mash1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000029">
        <property type="entry name" value="Achaete-scute family bHLH transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF153">
        <property type="entry name" value="ACHAETE-SCUTE FAMILY BHLH TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="82"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="200" mass="21961" checksum="3490E523D04A185F" modified="2020-06-17" version="1">MDLAAKMEINVNQQQLMPPACFFSAAAAQSIQLSPSGSSQSSGKSGAKQPKRQRSSSPELLRCKRRLNFAGFGYSLPQQQPHAVARRNERERNRVKLVNNGFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQSGVLSPTMSQGYSADMNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTNWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BI96</accession>
    <name>A0A673BI96_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box protein D2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">foxd7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BI96"/>
    <dbReference type="Ensembl" id="ENSSORT00005043373.1">
        <property type="protein sequence ID" value="ENSSORP00005042296.1"/>
        <property type="gene ID" value="ENSSORG00005019605.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BI96"/>
    <dbReference type="OrthoDB" id="5402974at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF361">
        <property type="entry name" value="FORKHEAD BOX PROTEIN D4-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="325" mass="35948" checksum="B8ED05E599AACBFC" modified="2020-06-17" version="1">MTLGLEIMEDIVIDVVGEGLKDNAEEQLLPLDESRTEQDRNSETLSSGSQSKDQDDYSPAPACPPPKTKGPTSVKPPYSYIALITMAILQSPKKRLTLSEICDFISHRFVYYREKFPAWQNSIRHNLSLNDCFVKMPREPGNPGKGNYWTLDPNSSDMFENGSFLRRRKRFKRQHFRFDPLKEQHLEPSGLHGFPHGAYGLGAAALQLPSLEIYPYIHHHAPSPCAPANIPPVSSILPALSSFFSRGALTAKAFLQSQPGIDAFSPAQCTAYSSPLTSSAAYASPALFHPAASPHFLSLHQEYQKLQTQRGAANLAKHASSVNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BII8</accession>
    <name>A0A673BII8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 410-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">znf410</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BII8"/>
    <dbReference type="Ensembl" id="ENSSORT00005043469.1">
        <property type="protein sequence ID" value="ENSSORP00005042391.1"/>
        <property type="gene ID" value="ENSSORG00005019653.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BII8"/>
    <dbReference type="OrthoDB" id="5977959at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000071">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000462">
        <property type="entry name" value="Zinc finger protein 410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000441">
        <property type="entry name" value="zinc finger protein 410 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF24">
        <property type="entry name" value="ZINC FINGER PROTEIN 410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="249"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="309"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="447" mass="48621" checksum="6C3B7001D65B3BE7" modified="2020-06-17" version="1">MLSDELDSKPELLVQFVQNASIPLVQGLEDPESKHPCLPLLAPAVSSLCSPLELTDGGLSHEPESSSLSEFGGVSERSPLVVQTHSHPRTSPSPPPILHDLQQSDSTSYVLLNLAKGITASSESLIFAADGAGEDDDEGVSSGDYGIDGSAPWYLRVQELAHDSLIAATRAQLARDAKASQDARATSISNGDHTHSLTSDGDKRELPSRTSRPLSKQILRCSFEGCCRTFTWPAHLKYHLKTHRNDRMFRCGAEGCGKSFYVLQRLQVHMRTHNGEKPFICKEKNCGKKFTTAGNLKNHRRIHTGEKPFLCEADGCGRSFAEYSSLRKHMLVHSGEKPHHCGICGKTFSQSGSRNVHMRKRHGEEGLNSETREAGEALTQSSLLEADGENGEGMVTMTTAVEPMNLHHAMLRSPGSADSVVVLSQPHELVTMTTEGHAYGDDVVALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJT6</accession>
    <name>A0A673BJT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gja4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BJT6"/>
    <dbReference type="FunCoup" id="A0A673BJT6">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043097.1">
        <property type="protein sequence ID" value="ENSSORP00005042025.1"/>
        <property type="gene ID" value="ENSSORG00005019485.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJT6"/>
    <dbReference type="OrthoDB" id="8878488at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF39">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="13">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="14">
        <location>
            <begin position="182"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="381" mass="42979" checksum="E3CA646C7D486E3E" modified="2020-06-17" version="1">MSRADWTFLEHLLEEGQEYSTGIGRVWLTVLFLFRMLVLGTAAESAWDDEQADFICNTKQPGCIAVCYDKAFPISHFRYFVLQVIFVSTPTIFFFGYVAIRAGREKNKEEGKDESGAECSAGLKNDAIVIDKNATKENAEEKKKKENGEKGKKCDRSSPEAPKLKGRLLCAYAFSILLKILLEAGFIIGLWFLYDGFFIAAKFECTRTPCPHTVDCFVSRPTEKTIFTIYTQVIAAISLLLNIIELFHLLRLAVSHRLEKRYRSERVEYLPRSKRVLAREEAPDFQMEPAQSYKAGSHGNLPPQGEVTGYHNSCESYRDLAGEVNWGPGESGGDLLPSYMNCMGAMSNSHSPIVIYKKHQHHTGKQTKGAHKGHSKQKHYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BK84</accession>
    <name>A0A673BK84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BLOC-1-related complex subunit 6 C-terminal helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">borcs6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029994752.1">
        <property type="nucleotide sequence ID" value="XM_030138892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BK84"/>
    <dbReference type="FunCoup" id="A0A673BK84">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043254.1">
        <property type="protein sequence ID" value="ENSSORP00005042180.1"/>
        <property type="gene ID" value="ENSSORG00005019550.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422527"/>
    <dbReference type="CTD" id="54785"/>
    <dbReference type="InParanoid" id="A0A673BK84"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019314">
        <property type="entry name" value="BORCS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046465">
        <property type="entry name" value="BORCS6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440:SF7">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10157">
        <property type="entry name" value="BORCS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLOC-1-related complex subunit 6 C-terminal helix" evidence="2">
        <location>
            <begin position="307"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="410" mass="44538" checksum="0A643264AFF97656" modified="2020-06-17" version="1">MSLSPVIGTDVPETANGVVTSVSAENGPHVPIVVKCGGSRLPCPGEESEDGPGQTDNHMDMDRLVYDGKGRLDAEADINDRTSSLSLYTTGDSDTHVTEEPPGAALQWDSALFTQAKDSERQTERQTETDGQEEEDGEKEKQDEEEDEKKWRKQNTAGRREEELSRHYSSSAPGPSTSSSSSPPPPLLPSDDTPCPPHVMAQVWVRNVRGMQDSKSLDEISQACGGGSGARGGGRGGQSEGRRATISSALELEGTVSHEGDLTNFITKNLEQKIKMSSKPSLDCSDSDCSGPIYRSRGLTRRPADIPPIDPTVLVDLQRHTQDVAHSVEMMMRSLNGTIQNMTALSVGYIQTYRDSVDSLGESVDMSIKGMYTLMARCEELDRSMQPIHTLAAQIRDIKRTLDALEAICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKL6</accession>
    <name>A0A673BKL6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Keratin, type I cytoskeletal 17-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115428090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030002785.1">
        <property type="nucleotide sequence ID" value="XM_030146925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BKL6"/>
    <dbReference type="FunCoup" id="A0A673BKL6">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043392.1">
        <property type="protein sequence ID" value="ENSSORP00005042315.1"/>
        <property type="gene ID" value="ENSSORG00005019613.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428090"/>
    <dbReference type="InParanoid" id="A0A673BKL6"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF180">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="73"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="77"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="182"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="48182" checksum="7B8AC78656BE7980" modified="2020-06-17" version="1">MSSSVRRFSSSMSLSSLPSLSRAQRAASVYGGYGGGSGGVWGSSSASTGLGSGFDLALLGGGGGGGGERIGNEKVTMQNLNDRLASYLEKVRTLESANDKLERQIRDWYDKQTPTVRDYSKYEAIIADLRKKISAASQDNARLMLQIDNARLAAEDFRVKFENELAVRMSVEADIGGLRKVLDELTMARSDLEMQVEGLKEELVYLKKNHEEELAALRGQMKSSSVNVEVDALPQVDLNKTLNEIRTQYEAIAEKNRREMEEWYKVKFDELNKQVASSTETLQTSRSEINDLKRTLQSLQIELQSQHSLKSALEEQLADTESRYSMQLNQLQSMVNALEVELSQVRADIKRQAADYQTLLDIKTRLELEIAEYRRLLDGEDLSRTVVKTEVKEVKPEPVRTQRTKIVVEEIVDGKVVSRTEDVQASVINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BM18</accession>
    <name>A0A673BM18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Catenin alpha-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ctnna1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BM18"/>
    <dbReference type="FunCoup" id="A0A673BM18">
        <property type="interactions" value="1924"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042355.1">
        <property type="protein sequence ID" value="ENSSORP00005041293.1"/>
        <property type="gene ID" value="ENSSORG00005019186.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BM18"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000006">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000007">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000008">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000011">
        <property type="entry name" value="Catenin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000012">
        <property type="entry name" value="Catenin alpha-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001033">
        <property type="entry name" value="Alpha_catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000633">
        <property type="entry name" value="Vinculin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914">
        <property type="entry name" value="ALPHA CATENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18914:SF24">
        <property type="entry name" value="CATENIN ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00805">
        <property type="entry name" value="ALPHACATENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00663">
        <property type="entry name" value="VINCULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="887"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="887"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="931" mass="103063" checksum="9C8643B8D513CA9E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BPY3</accession>
    <name>A0A673BPY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homolog subfamily B member 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Endoplasmic reticulum DNA J domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115430611</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins. In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1. Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with HSPA5/BiP; interaction is direct. Interacts with ERN1/IRE1 (via the luminal region). Interacts with DERL1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030006576.1">
        <property type="nucleotide sequence ID" value="XM_030150716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BPY3"/>
    <dbReference type="Ensembl" id="ENSSORT00005045931.1">
        <property type="protein sequence ID" value="ENSSORP00005044801.1"/>
        <property type="gene ID" value="ENSSORG00005020600.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430611"/>
    <dbReference type="InParanoid" id="A0A673BPY3"/>
    <dbReference type="OrthoDB" id="376357at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025420378" description="DnaJ homolog subfamily B member 9" evidence="7">
        <location>
            <begin position="24"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="26"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="236" mass="27691" checksum="013BFCAA60FA6837" modified="2020-06-17" version="1">MATTQSVLLLAVHILLVSEFILAKRDYYDILGVPKDASDRQIKKAFHRLALKYHPDRNKHPDAEAKFREIAEAYETLSDDKRRQEYDHFEHQASPGEGYRGGGGGGNANYNFKQHFQSFNFNFDDIFKDFEPFGQQHHQHHFHTHSHSNSQAHQKRDFNSHFQAHQEAMNRQRRQFQQGSFGGGLFDDMFEDLEKMFTFHPHNTRTENRFQNSGKQHCRTVTQRRGNMVTTFTDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQY8</accession>
    <name>A0A673BQY8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IgLON family member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. IgLON family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BQY8"/>
    <dbReference type="FunCoup" id="A0A673BQY8">
        <property type="interactions" value="481"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045315.1">
        <property type="protein sequence ID" value="ENSSORP00005044195.1"/>
        <property type="gene ID" value="ENSSORG00005020375.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQY8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000305">
        <property type="entry name" value="neurotrimin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050876">
        <property type="entry name" value="IgLON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757:SF12">
        <property type="entry name" value="IGLON FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757">
        <property type="entry name" value="IGLON FAMILY OF IMMUNOGLOBULIN SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="30"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="126"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="227" mass="25678" checksum="D53B7BF9CDE373DD" modified="2020-06-17" version="1">MRSDFSIHINKVQVTDEGPYTCTFQANNKPRITHVYLIVQVPARIVNISKNVSVNEGENVNLYCLAVGRPEPTVTWKDQKYGLVSDGEFLDITEIKRQQAEDYECITNNGVGASDQRKVKVTVNYPPMITDVKNMPAHLGKTAILRCEAMAVPPASFEWYRDDHRPVESDNTLRIKNEKTRSLLLFTNVTEKHFGNYTCFASNRLGASNASMLLFRKLSKCTSTQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BS04</accession>
    <name>A0A673BS04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115416405</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BS04"/>
    <dbReference type="Ensembl" id="ENSSORT00005044461.1">
        <property type="protein sequence ID" value="ENSSORP00005043367.1"/>
        <property type="gene ID" value="ENSSORG00005020028.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BS04"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051843">
        <property type="entry name" value="CPA1_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102:SF22">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 9B2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="7">
        <location>
            <begin position="80"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="486" mass="51374" checksum="987BE58C5D3574D3" modified="2020-06-17" version="1">MKMASSSSSSCCSVCVRLKDRCPRPQGLLNLLITKVCLFMLLFGVVWSISGTQCLPGGNLFGIVVLFICSVLGGKLVGMIQLPTLPPFPPLLGMLLAGLLLRNVPYITDAVFVDTHWSSALRNIALSIILTRAGLGLDPTALQRLKAVCVRVAVGPCVVEACVVAVVSHFLLGLPWVWGFILGFVLAAVSPAVVVPSMLLLQREGYGVEKGIPTLLMAAGSFDDILAITGFSTCLGIAFSTGSTWMNILKGLLEVVGGIIAGLVLGLFLCWFPSKDQDLVLRRTLMLLGLSIFSVFCSHVIGFAGAGGLCTLVLSFLAALGWKADKAPVAAMVGRSWDVFQPLLFGLIGAEITIATLSPSTVGLGLACISVGLLVRLLVTFLLVHFGGFNLKEKLFISVAWLPKATVQAAIGSKALDLAREEGNDAFMKFGLDVLTLAVLAILTTAPIGALGIGLAGPRLLARQVKGQHTQFRFRMVTNLANASPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BS26</accession>
    <name>A0A673BS26_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serrate RNA effector molecule homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115416386</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029985982.1">
        <property type="nucleotide sequence ID" value="XM_030130122.1"/>
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        <property type="protein sequence ID" value="ENSSORP00005043387.1"/>
        <property type="gene ID" value="ENSSORG00005020032.1"/>
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    <dbReference type="OrthoDB" id="342064at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007042">
        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021933">
        <property type="entry name" value="SERRATE/Ars2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
        <property type="entry name" value="ARS2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SERRATE_Ars2_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="148"/>
            <end position="257"/>
        </location>
    </feature>
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        <location>
            <begin position="635"/>
            <end position="831"/>
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        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
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        <location>
            <begin position="270"/>
            <end position="420"/>
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        <location>
            <begin position="567"/>
            <end position="588"/>
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        <location>
            <begin position="748"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="86"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="106"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="271"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="299"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="338"/>
            <end position="353"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="367"/>
            <end position="378"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="386"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="399"/>
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        <location>
            <begin position="787"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="838"/>
            <end position="852"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="860" mass="97474" checksum="DBE7022E99AEDBD9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTX0</accession>
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        <submittedName>
            <fullName evidence="11">cAMP regulated phosphoprotein 19</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2 9">Belongs to the endosulfine family.</text>
    </comment>
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        <property type="match status" value="1"/>
    </dbReference>
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            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="108" mass="11954" checksum="A59CCF34C6D7F33C" modified="2020-06-17" version="1">VPRTLMGTENSECEEKMISPEKAEEAKLKARYPNLGNKPGGSDLLRKRLQKGQKYFDSGDYNMAKAKIKNKQLPTAAPEKTEITGDHIPTPQDLPQRKTSLVTSKLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUI0</accession>
    <name>A0A673BUI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 157</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the CFAP157 family.</text>
    </comment>
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        <property type="gene ID" value="ENSSORG00005021304.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUI0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:sperm axoneme assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038844">
        <property type="entry name" value="CFAP157"/>
    </dbReference>
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        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31954:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010841"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="274" mass="32116" checksum="F49004378292861D" modified="2020-06-17" version="1">NKQFIHSSIRYLNSRISSLCGRYQLKCDELERQKKDFSSQYNVLEKEKRDIVEYLKCSLLEKEEEVNMLHERLENERQTAQKEQDTLQQQHRLLIQELHHRNDELAAENMTLVQKLDALEEFQKQREQLVSKMESLKNQVASQEQEHKAAIHSLEMKALLERNRLEKEMQSQAASMAAEVQHLVEQNLPEATRLAIEENKEVRSRCSQLSDHACGLLKVNTALQTRKTKLRLDVDILEQMVSDISRKSCIQKKVRPKNTESKAIITDSDHICQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVL4</accession>
    <name>A0A673BVL4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="protein sequence ID" value="ENSSORP00005045870.1"/>
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        <property type="component" value="Chromosome 24"/>
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        <property type="entry name" value="Potassium voltage-gated channel, subfamily H (Eag-related), member 1"/>
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        <property type="entry name" value="KCNH_animal-type"/>
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        <location>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="428" mass="49105" checksum="7503464961732E8A" modified="2020-06-17" version="1">MKHSLSFVSFMYGDLTDKNTIDKIKETFDTYESNFFEVLLYAKNRTPIWLYMQVAPIRNENNKVVLFLCTFRDITLFKQPIDDESTRGWTKFARLTRALTTNRNLVQQLAPLSKAEVNHKPSRLAEVLQLGSDILPQYKQEAPKTPPHIILHYCTFKTTWDWVILILTFYTAIMVPYNVSFKTKQNNLAWLVVDSVVDVIFLIDIVLNFHTTFVGPAGEVISDAKLIRMNYMKTWFVIDLLSCLPYDIINAFEHVDDGLSSLFSSLKVIRLLRLGRVARKLDHYLEYGAAVLVLLVCVFGLVAHWLACIWYSIGDYEVIDETTNTIKTDSWLYQLAINIGTPYRYNTSGTGQWEGGPNKDTLYISSLYFTMTSLTTIGFGNIAPTTDGEKIFSVAMMMVGCEYKVFFYIKPTVNRTVGFGWDTGICYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZH0</accession>
    <name>A0A673BZH0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Prolactin-releasing peptide receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">G-protein coupled receptor 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC115426065</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for prolactin-releasing peptide (PrRP). Implicated in lactation, regulation of food intake and pain-signal processing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts through its C-terminal region with the PDZ domain-containing proteins GRIP1, GRIP2 and PICK1. Interacts with PDZ domains 4 and 5 of GRIP1 and with the PDZ domain of PICK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030144009.1"/>
    </dbReference>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005046414.1"/>
        <property type="gene ID" value="ENSSORG00005021250.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426065"/>
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    <dbReference type="OrthoDB" id="5975336at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:neuron projection"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
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    </dbReference>
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        <property type="term" value="F:neuropeptide Y receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15394">
        <property type="entry name" value="7tmA_PrRP_R"/>
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    </dbReference>
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        <property type="entry name" value="Prolactin releasing hormone receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001402">
        <property type="entry name" value="Prolrel_pep_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF14">
        <property type="entry name" value="PROLACTIN RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="PRPRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
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        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="307"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="72"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055229"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="364" mass="41149" checksum="5A4BE7EBD5FA82C0" modified="2020-06-17" version="1">MEGAGSGWATELTPLYVMHDGNENNTGKVFEVALQNGSSKRSPQFVGVELLQSFKLLIIPCYTLVALVGIFGNYLLLYVICRTRKMHNVTNFFIGNLAFSDMLMCATCVPFTLAYAFNPNGWVFGRFMCYLVYLIQPVTVYVSVFTLTAIGVDRYYATVHPLKKRISVLACTYLLSGIWLLSCGLVAPAVAHTYHVEFKNEGFTICEEFWMGQEKERLAYAYGTLFITYVLPLSALCISYLCISVKLRNCVVPGHHTQSQAEAQRMRKRKTFRLVSLVVAAFGVCWMPISVFNVLRDIDIGLIDKRYFLLIQLLCHLCAMSSSCCNPFLYAWLHDRFRAELRKMFTCRRRIGISANNCATASVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C086</accession>
    <name>A0A673C086_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine rich repeat containing 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115413728</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSSORP00005048656.1"/>
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        <property type="entry name" value="SDS22/Internalin_LRR"/>
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        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
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            <begin position="265"/>
            <end position="288"/>
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            <end position="1115"/>
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            <begin position="269"/>
            <end position="288"/>
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    </feature>
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        <location>
            <begin position="1097"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1257" mass="143153" checksum="4208F1F0A3C7A5F5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1U5</accession>
    <name>A0A673C1U5_9TELE</name>
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        <recommendedName>
            <fullName evidence="13">Synaptotagmin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">syt1a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="13">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Chromaffin granule membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">Secretory vesicle</location>
            <location evidence="1 13">Synaptic vesicle membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C1U5"/>
    <dbReference type="FunCoup" id="A0A673C1U5">
        <property type="interactions" value="492"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005046328.1"/>
        <property type="gene ID" value="ENSSORG00005021215.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:chromaffin granule membrane"/>
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        <property type="term" value="C:dense core granule"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
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    </dbReference>
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        <property type="term" value="P:synaptic vesicle endocytosis"/>
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    </dbReference>
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        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
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    </dbReference>
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        <property type="entry name" value="C2B_Synaptotagmin-1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF239">
        <property type="entry name" value="SYNAPTOTAGMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="139"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="270"/>
            <end position="403"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
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        <location>
            <begin position="111"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004349"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU367154"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="419" mass="47200" checksum="00DFE5F354BF6D0A" modified="2020-06-17" version="1">MNESRREALAAPATTASSSVAGSNTTNVAGPGTGEKDEAFSKLKDKFMNELNKIPLPSWAIVSIAFVAIILVLACCFCICKKWIFKKKNKKKGKDKGKNAINMKDVKDGAKTEALKDEEDAETGLTETEKEAEPKEDQKLGKLQYSLDYNFTENTLIVGIIQAAELPAMDMGGTSDPYVKVYLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYVELGGKTLVMTVYDFDRFSKHDAIGDIKVPMNKVDFSHITEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQIVITVLDYDKIGKNDAIGKVFVGLNSSGTELRHWSDMLANPRRPIAQWHVLKPEEEVDAQLSTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2B4</accession>
    <name>A0A673C2B4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C2B4"/>
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        <property type="protein sequence ID" value="ENSSORP00005048135.1"/>
        <property type="gene ID" value="ENSSORG00005021978.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C2B4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="2">
        <location>
            <begin position="37"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
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</entry>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="585"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="742"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="288"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="585"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="637"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="742"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="771"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="797"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="814" mass="85712" checksum="B02F0F00939D7C0B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C963</accession>
    <name>A0A673C963_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C963"/>
    <dbReference type="Ensembl" id="ENSSORT00005053369.1">
        <property type="protein sequence ID" value="ENSSORP00005052126.1"/>
        <property type="gene ID" value="ENSSORG00005023515.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C963"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="119" mass="13458" checksum="EA17CB2E4A6A64EA" modified="2020-06-17" version="1">RKKKRTKNEKKREKKRKLPPDCCLSESFTSPSCTLGVDLRRGRRRFSGNLQLPPLSWRQGERSRTPDEEVMTRPTSLPFGAPPRIDITPVDPECEPTRTNQTLTVDTRGGVTHGTLICG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBS9</accession>
    <name>A0A673CBS9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TAFA chemokine like family member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAFA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBS9"/>
    <dbReference type="Ensembl" id="ENSSORT00005052761.1">
        <property type="protein sequence ID" value="ENSSORP00005051530.1"/>
        <property type="gene ID" value="ENSSORG00005023272.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBS9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Chemokine-like_TAFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040329">
        <property type="entry name" value="TAFA-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31878:SF0">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31878">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12020">
        <property type="entry name" value="TAFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="14099" checksum="0735F6888665FF33" modified="2020-06-17" version="1">MRMLSLSVATAGTSVWMNPDRSPLPWLAAGTCEIVTLDRDSSQPRRTIARQTARCACKKGQIAGTTRARPACVDASIVRKKQWCEMVPCLEDEGCDLLVNKSGWTCTQPGGRVKTTTVSSYSLLLPQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CDQ6</accession>
    <name>A0A673CDQ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein 4</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3-keto acyl-CoA synthase ELOVL4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">ELOVL fatty acid elongase 4</fullName>
            <shortName evidence="10">ELOVL FA elongase 4</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Very long chain 3-ketoacyl-CoA synthase 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Very long chain 3-oxoacyl-CoA synthase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">elovl4b</name>
        <name evidence="10" type="synonym">ELOVL4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that specifically elongates C24:0 and C26:0 acyl-CoAs. May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family. ELOVL4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A673CDQ6"/>
    <dbReference type="FunCoup" id="A0A673CDQ6">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052672.1">
        <property type="protein sequence ID" value="ENSSORP00005051444.1"/>
        <property type="gene ID" value="ENSSORG00005023246.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CDQ6"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03204">
        <property type="entry name" value="VLCF_elongase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033678">
        <property type="entry name" value="ELOVL4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF61">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="268"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="305" mass="35587" checksum="043B549A64044105" modified="2020-06-17" version="1">MEVVTHFVNDTVEFYKWSLTIADKRVENWPMMSSPFPTLAISCLYLLFLWAGPRYMQDRQPYTLRKTLIVYNFSMVVLNFYIAKELLLGSRAARYSYLCQPVNYSNDVNEVRIASALWWYYISKGVEFLDTVFFILRKKFNQVSFLHVYHHCTMFILWWIGIKWVPGGQSFFGATINSSIHVLMYGYYGLAALGPQMQKYLWWKKYLTIIQMIQFHVTIGHAGHSLYTGCPFPAWMQWALIGYAVTFIILFANFYYHAYRRKPFSSQKGGKAVANGTTMVTNGHSKAEEVEDDGKKQKKGRAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CHS8</accession>
    <name>A0A673CHS8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Four and a half LIM domains protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fhl5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CHS8"/>
    <dbReference type="FunCoup" id="A0A673CHS8">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056424.1">
        <property type="protein sequence ID" value="ENSSORP00005055146.1"/>
        <property type="gene ID" value="ENSSORG00005024644.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CHS8"/>
    <dbReference type="OrthoDB" id="274660at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09343">
        <property type="entry name" value="LIM1_FHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09346">
        <property type="entry name" value="LIM3_FHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09347">
        <property type="entry name" value="LIM4_FHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000013">
        <property type="entry name" value="Four and a half LIM domains 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000052">
        <property type="entry name" value="Four and a half LIM domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000030">
        <property type="entry name" value="Four and a half LIM domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000048">
        <property type="entry name" value="Four and a half LIM domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056807">
        <property type="entry name" value="LIM_FHL1/2/3/5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24205:SF7">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25076">
        <property type="entry name" value="LIM_FHL2-3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="40"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="162"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="224"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="32328" checksum="A32730AF620E79B3" modified="2020-06-17" version="1">MSTSERFDCHYCKDSLLGKKYIMKEDTQYCTKCYENLFANCCEGCSLPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLVEKAFAAKDDLLLCTECYAHDYSSKCTTCKKTVMPGSRKMEYKGNSWHETCFLCHRCQQPIGTKSFIPKDTGYFCVPCFEKQFAYQCCACKKAITTGGVTYQDKPWHRECFLCIGCRKQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKAITSLAGAKYISFEERQWHSECFTCMQCSVSLVGRGFLTQRDNILCTDCGREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CIK1</accession>
    <name>A0A673CIK1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plexin-A2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">plxna2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Coreceptor for SEMA3A and SEMA6A. Necessary for signaling by SEMA6A and class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A673CIK1">
        <property type="interactions" value="904"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055291.1">
        <property type="protein sequence ID" value="ENSSORP00005054020.1"/>
        <property type="gene ID" value="ENSSORG00005022538.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CIK1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001763">
        <property type="term" value="P:morphogenesis of a branching structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01179">
        <property type="entry name" value="IPT_plexin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01181">
        <property type="entry name" value="IPT_plexin_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12790">
        <property type="entry name" value="RasGAP_plexin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000005">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000006">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000123">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000006">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000071">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000339">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000695">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000018">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000032">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TIG2_plexin"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PSI"/>
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        <property type="entry name" value="E set domains"/>
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        <property type="entry name" value="GTPase activation domain, GAP"/>
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    <keyword evidence="9 17" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
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        <location>
            <begin position="1258"/>
            <end position="1280"/>
        </location>
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            <begin position="39"/>
            <end position="525"/>
        </location>
    </feature>
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        <location>
            <begin position="1284"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1914" mass="213471" checksum="31A74400B4FBCEC3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQ10</accession>
    <name>A0A673CQ10_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane protein 131</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tmem131</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM131 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005057661.1">
        <property type="protein sequence ID" value="ENSSORP00005056365.1"/>
        <property type="gene ID" value="ENSSORG00005024938.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_TMEM131L_3"/>
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        <property type="entry name" value="Ig_TMEM131L_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039877">
        <property type="entry name" value="TMEM131-like"/>
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    <dbReference type="InterPro" id="IPR056311">
        <property type="entry name" value="TMEM131_Ig_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055437">
        <property type="entry name" value="TMEM131L_Ig_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022113">
        <property type="entry name" value="TMEM131L_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22050">
        <property type="entry name" value="RW1 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22050:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24495">
        <property type="entry name" value="Ig_TMEM131_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24498">
        <property type="entry name" value="Ig_TMEM131L_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24499">
        <property type="entry name" value="Ig_TMEM131L_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24501">
        <property type="entry name" value="Ig_TMEM131L_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12371">
        <property type="entry name" value="TMEM131_like_N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein 131-like N-terminal" evidence="9">
        <location>
            <begin position="90"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM131 second Ig-like" evidence="10">
        <location>
            <begin position="189"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM131L third Ig-like" evidence="11">
        <location>
            <begin position="416"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM131L fourth Ig-like" evidence="12">
        <location>
            <begin position="816"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM131L fifth Ig-like" evidence="13">
        <location>
            <begin position="1004"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1180"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1277"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1534"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1668"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1710"/>
            <end position="1746"/>
        </location>
    </feature>
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        <location>
            <begin position="1245"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1294"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1309"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1319"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1346"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1668"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1718"/>
            <end position="1746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF24501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1772" mass="195093" checksum="BF365F59FC7D9451" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CTT5</accession>
    <name>A0A673CTT5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">BRCA1-associated protein</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">BRAP2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Impedes mitogenic signal propagation</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase BRAP2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">brap</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030005921.1">
        <property type="nucleotide sequence ID" value="XM_030150061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CTT5"/>
    <dbReference type="Ensembl" id="ENSSORT00005056633.1">
        <property type="protein sequence ID" value="ENSSORP00005055353.1"/>
        <property type="gene ID" value="ENSSORG00005024683.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430171"/>
    <dbReference type="CTD" id="8315"/>
    <dbReference type="InParanoid" id="A0A673CTT5"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16457">
        <property type="entry name" value="RING-H2_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12718">
        <property type="entry name" value="RRM_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000206">
        <property type="entry name" value="BRCA1-associated protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000159">
        <property type="entry name" value="BRCA1-associated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011422">
        <property type="entry name" value="BRAP2/ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034932">
        <property type="entry name" value="BRAP2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047243">
        <property type="entry name" value="RING-H2_BRAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07576">
        <property type="entry name" value="BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="271"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="UBP-type" evidence="22">
        <location>
            <begin position="308"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="75"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="561"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="445"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="591"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="601" mass="67731" checksum="3498627E6669BAB7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CV84</accession>
    <name>A0A673CV84_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Ankyrin 3a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115433988</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <location evidence="13">Sarcolemma</location>
            <location evidence="13">T-tubule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005058706.1">
        <property type="protein sequence ID" value="ENSSORP00005057389.1"/>
        <property type="gene ID" value="ENSSORG00005025040.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CV84"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08803">
        <property type="entry name" value="Death_ank3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000003">
        <property type="entry name" value="Ankyrin, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000001">
        <property type="entry name" value="Ankyrin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000002">
        <property type="entry name" value="Ankyrin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000002">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000001">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000002">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2660:FF:000001">
        <property type="entry name" value="Ankyrin-3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037971">
        <property type="entry name" value="Ank3_Death"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040745">
        <property type="entry name" value="Ankyrin_UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051165">
        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF74">
        <property type="entry name" value="ANKYRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17809">
        <property type="entry name" value="UPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
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        <property type="entry name" value="Tudor_FRM1"/>
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    <dbReference type="PANTHER" id="PTHR10603">
        <property type="entry name" value="FRAGILE X MENTAL RETARDATION SYNDROME-RELATED PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10603:SF6">
        <property type="entry name" value="RNA-BINDING PROTEIN FXR1"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Agenet-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
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    <feature type="domain" description="Agenet-like" evidence="8">
        <location>
            <begin position="63"/>
            <end position="115"/>
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            <begin position="378"/>
            <end position="681"/>
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        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="458"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="479"/>
            <end position="496"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="579"/>
            <end position="589"/>
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            <end position="616"/>
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            <begin position="635"/>
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            <begin position="672"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004331"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006633"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="681" mass="75826" checksum="F49D503FF1EB8FCF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5I7</accession>
    <name>A0A672Y5I7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Neuroendocrine convertase 1</fullName>
            <ecNumber evidence="6">3.4.21.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Prohormone convertase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Proprotein convertase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">pcsk1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.</text>
            <dbReference type="EC" id="3.4.21.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030134382.1"/>
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        <property type="protein sequence ID" value="ENSSORP00005001393.1"/>
        <property type="gene ID" value="ENSSORG00005000786.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419537"/>
    <dbReference type="CTD" id="5122"/>
    <dbReference type="InParanoid" id="A0A672Y5I7"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034774">
        <property type="term" value="C:secretory granule lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000010">
        <property type="entry name" value="Putative neuroendocrine convertase 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase S8, pro-domain"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
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        <property type="entry name" value="P_dom"/>
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    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
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    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
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    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
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        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022005">
        <property type="entry name" value="Proho_convert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12177">
        <property type="entry name" value="Proho_convert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 21 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 21 22" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 21 22" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025656350" description="Neuroendocrine convertase 1" evidence="24">
        <location>
            <begin position="28"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="25">
        <location>
            <begin position="467"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="636"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="685"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="642"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="685"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="774" mass="86314" checksum="17DC28479235C964" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAY3</accession>
    <name>A0A672YAY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115424730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YAY3"/>
    <dbReference type="Ensembl" id="ENSSORT00005001667.1">
        <property type="protein sequence ID" value="ENSSORP00005001619.1"/>
        <property type="gene ID" value="ENSSORG00005001012.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAY3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025602433" description="B30.2/SPRY domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="636"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="823" mass="93081" checksum="DB2818E7EB468BED" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YB65</accession>
    <name>A0A672YB65_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor MafG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">V-maf musculoaponeurotic fibrosarcoma oncogene homolog G</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YB65"/>
    <dbReference type="Ensembl" id="ENSSORT00005000556.1">
        <property type="protein sequence ID" value="ENSSORP00005000530.1"/>
        <property type="gene ID" value="ENSSORG00005000350.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YB65"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045604">
        <property type="term" value="P:regulation of epidermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14717">
        <property type="entry name" value="bZIP_Maf_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000011">
        <property type="entry name" value="Transcription factor MafG, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="13">
        <location>
            <begin position="64"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="169" mass="18963" checksum="64703A8C1B3FC269" modified="2020-06-17" version="1">MRIHLLKNPPAQEVCAKTVNKESQGVKREPGENGTSLTDEELVTMSVRELNQHLRGLTKEEILQLKQRRRTLKNRGYAASCRVKRVTQKEELEKQKAQLQQEVDKLANENASMRVELDALRSKYEALQTFARTVARRVASSVIGPLIPGKVATATSVITIVKSKTDARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YC94</accession>
    <name>A0A672YC94_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fas (tnfrsf6)-associated via death domain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadd</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029985562.1">
        <property type="nucleotide sequence ID" value="XM_030129702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YC94"/>
    <dbReference type="FunCoup" id="A0A672YC94">
        <property type="interactions" value="1398"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000302.1">
        <property type="protein sequence ID" value="ENSSORP00005000281.1"/>
        <property type="gene ID" value="ENSSORG00005000215.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416002"/>
    <dbReference type="CTD" id="8772"/>
    <dbReference type="InParanoid" id="A0A672YC94"/>
    <dbReference type="OrthoDB" id="100767at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031265">
        <property type="term" value="C:CD95 death-inducing signaling complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089720">
        <property type="term" value="F:caspase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005123">
        <property type="term" value="F:death receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08336">
        <property type="entry name" value="DED_FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000059">
        <property type="entry name" value="Fas-associated via death domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016729">
        <property type="entry name" value="FADD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077:SF10">
        <property type="entry name" value="FAS-ASSOCIATED DEATH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077">
        <property type="entry name" value="FAS-ASSOCIATING DEATH DOMAIN-CONTAINING PROTEIN FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00031">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DED" evidence="2">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="101"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21770" checksum="4DE70B5367289B3D" modified="2020-06-17" version="1">MSDSLEFNTVLMDISNQLTAEELEKLKYLCDKDIGKRDLEKSTTGLKLFQLLKHRNKLGPGNTDYLSDRLGRIHRQDLRDKLEGIDGQTENQPDEAERARLNVATEVIAENLGKSWRKLGRKLGLSEVKLDSINAKHQFDLEEITVEMLKEWRKSQGARARVEDLTKALRDCQLKLTAEKVEARLETPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDG9</accession>
    <name>A0A672YDG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Progestin and adipoQ receptor family member IIIa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">paqr3a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YDG9"/>
    <dbReference type="FunCoup" id="A0A672YDG9">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001042.1">
        <property type="protein sequence ID" value="ENSSORP00005001011.1"/>
        <property type="gene ID" value="ENSSORG00005000611.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDG9"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF15">
        <property type="entry name" value="PROGESTIN AND ADIPOQ RECEPTOR FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39494" checksum="88013474F82C4BC6" modified="2020-06-17" version="1">MRSAYFKPQNGTHTSYTKLKASGYSTAGSAGSAMPQKVQKNAQTAHYIELGGYQYWPVLVPRGIRLYTYEQIPVFLRENPYITDGYRAYLPSRLCIKSLFILSNETVNIWSHLLGFLLFFCLAVYNMASVLPDIGASREDYVIYSIGLFCFQLCMLCSVGYHLFCCHRSEKTSRRWMALDYAGVSIGILGCYVPGVFYTFYCNNYWRQVYLVTVLAMILAVFFAQIHPHYLSKQWKQLRSLIFCSVAGYGLIPTVHWICITGGFSSELVQAFIPRVLGMYFIAALALIFYVSKVPERYFPGQLNYVGSSHQVWHVLLVLMFYWWHQTSGFIMAYRHNQPCPEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YEV8</accession>
    <name>A0A672YEV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">trypsin</fullName>
            <ecNumber evidence="13">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115416716</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A672YEV8"/>
    <dbReference type="FunCoup" id="A0A672YEV8">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003154.1">
        <property type="protein sequence ID" value="ENSSORP00005003060.1"/>
        <property type="gene ID" value="ENSSORG00005001851.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YEV8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000069">
        <property type="entry name" value="Hyaluronan-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000001">
        <property type="entry name" value="Urokinase-type plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF40">
        <property type="entry name" value="HYALURONAN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0420">Kringle</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 16" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="47"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="124"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="20">
        <location>
            <begin position="165"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="21">
        <location>
            <begin position="356"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="251"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
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        <source>
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        </source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="604" mass="65743" checksum="B93D9B207423CB60" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF09</accession>
    <name>A0A672YF09_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YF09"/>
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        <property type="protein sequence ID" value="ENSSORP00005002186.1"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="SPERACTRCPTR"/>
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    </dbReference>
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        <property type="entry name" value="SRCR-like"/>
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    </dbReference>
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        <property type="entry name" value="SRCR_2"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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    <keyword evidence="3" id="KW-0732">Signal</keyword>
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        <location>
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    </feature>
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    </feature>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="32619" checksum="17D394B3DA7C9228" modified="2020-06-17" version="1">MKRHDGNWKPVEGYHWYRKLGNRVCAELDCGSAVSVRRRDTVLDLDNWWIRSDCDESELSDCFTSETSLSSFLELNCSDSVRLVNGSSVCSGRLKVRSNQSRSWSSVCEGHLDLHGAQVVCRELGCGAPGLLQGVLYDEAEAPVVQTFQCEGHESALLDCGSLDSETCSSGTAVDLTCTDPDDVRLVGGDSCCNGEVEIKYHGEWRYMEYGYYSSEPWTLKAADVICRRLDCGSAVSRRRSDPYGPLWVISPICLLSTSTLADCVRTDSRSIETTMSLTCSGKSTDDDPDSVRVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFC7</accession>
    <name>A0A672YFC7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox B1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YFC7"/>
    <dbReference type="FunCoup" id="A0A672YFC7">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002574.1">
        <property type="protein sequence ID" value="ENSSORP00005002511.1"/>
        <property type="gene ID" value="ENSSORG00005001495.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFC7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000113">
        <property type="entry name" value="homeobox protein Hox-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="HD_metazoa"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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</entry>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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    </evidence>
    <sequence length="898" mass="102274" checksum="FCFAD36BB6DC41FA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ85</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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        <text evidence="2 6">Belongs to the heparin-binding growth factors family.</text>
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        <property type="term" value="P:negative regulation of endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Fibroblast growth factor"/>
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        <property type="entry name" value="Fibroblast_GF_fam"/>
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        <property type="entry name" value="IL1/FGF"/>
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        <property type="entry name" value="IL1HBGF"/>
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        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cytokine"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0339">Growth factor</keyword>
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    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="26336" checksum="E83534D5D389902E" modified="2020-06-17" version="1">MRSTDLLEKAVTLTQFVQVFDKCEGKRTPKWSYQIRSETFGVDGQVRGCICNTGAVTDQLSRRQVRVYQLYSRTSGKHVQIQGKRVTATAEDGNVYARLFVETDTFGSRVRIRGAESGRYLCMNRKGKLVGKPKGRSRDCIFTEIVLENNYTAFQNAKYEGWYVAFTRKGRPIKASRTRENQREVHFIKRLHTGPPPFPNTDQSKHFEFIRFPATRRAKRNRKSHTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLK6</accession>
    <name>A0A672YLK6_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">TIL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YLK6"/>
    <dbReference type="Ensembl" id="ENSSORT00005005561.1">
        <property type="protein sequence ID" value="ENSSORP00005005403.1"/>
        <property type="gene ID" value="ENSSORG00005003207.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLK6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052749">
        <property type="entry name" value="Alpha-tectorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160">
        <property type="entry name" value="ALPHA-TECTORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160:SF9">
        <property type="entry name" value="PROTEIN PRY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="TIL" evidence="3">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="108" mass="11853" checksum="0BEA57CD2C5EB3A8" modified="2020-06-17" version="1">MYCNFSFINLFTSLYFMHYLFYPLLYSVLECPERPLACPPNSSYSLCVSSCPETCLGAVGPPGCEDVCVEGCECNPGFILSNDKCVALKDCGCVDTSGTYHPVSRSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLU8</accession>
    <name>A0A672YLU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLU8"/>
    <dbReference type="FunCoup" id="A0A672YLU8">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005833.1">
        <property type="protein sequence ID" value="ENSSORP00005005591.1"/>
        <property type="gene ID" value="ENSSORG00005003385.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLU8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF287">
        <property type="entry name" value="CADHERIN-RELATED NEURONAL RECEPTOR VARIABLE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="768"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="43"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="128"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="235"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="320"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="428"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="533"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="656"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="870"/>
            <end position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="937" mass="102847" checksum="17953176DC9DFC6B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YN48</accession>
    <name>A0A672YN48_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">FYVE and coiled-coil domain containing 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115436803</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
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            <authorList>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        </source>
    </evidence>
    <sequence length="1463" mass="167079" checksum="2738CF2FC678F5F5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YP13</accession>
    <name>A0A672YP13_9TELE</name>
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        <submittedName>
            <fullName evidence="15">Glycerophosphodiester phosphodiesterase domain containing 3a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gdpd3a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-O-(1Z-octadecenyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H2O = 1-O-(1Z-octadecenyl)-sn-glycero-3-phosphate + N-hexadecanoylethanolamine + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71464"/>
            <dbReference type="ChEBI" id="CHEBI:137009"/>
            <dbReference type="ChEBI" id="CHEBI:137017"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:53185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphate + choline + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:77580"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:41144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N,1-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(9Z-octadecenoyl) ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71466"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85222"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:56461"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:2700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85223"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:45545"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoylethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71464"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:53169"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YP13"/>
    <dbReference type="FunCoup" id="A0A672YP13">
        <property type="interactions" value="14"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005006278.1"/>
        <property type="gene ID" value="ENSSORG00005003718.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08612">
        <property type="entry name" value="GDPD_GDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF3">
        <property type="entry name" value="LYSOPHOSPHOLIPASE D GDPD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="14">
        <location>
            <begin position="38"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="314" mass="35887" checksum="B47CCDE22367291E" modified="2020-06-17" version="1">MSFLYFILPALGGYTLTSLYLLKNPHIIHKRKRTAFYCTHISHRGGCGERIESTMEAFTHAVEQGTQMIELDCHLTHDGHVVVSHDENLLRQTGHDVTISSLNLQDLPLYKERLEVTFKAGHYSSGADRQFALLEDLFRKFPAIPVSIEIKENNSPLIEKVSDLVKRYNREDITVWASVNSSIMKKCCRVNDSMPYSFSMNRGVLLLLLFYSGLLPFVPLGESLLQFYLPRVINRTFIPEKRILRNSLLVSLLEKVTMRRSLFKHLAARGIQVHLFVCNKDEDIKAAFEVGATGVMTDYPSLLTSYLCRNRSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YP90</accession>
    <name>A0A672YP90_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YP90"/>
    <dbReference type="Ensembl" id="ENSSORT00005006655.1">
        <property type="protein sequence ID" value="ENSSORP00005006399.1"/>
        <property type="gene ID" value="ENSSORG00005003771.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YP90"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="216"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="326"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="367" mass="40081" checksum="C30EBE10116D0F44" modified="2020-06-17" version="1">SIKEEWEKLSPEYLRNTCASFRKRVVSSDSASSVICWQADTGQKVKQFHRCHGNTEISTMALDGTQTRLFTAGADGEVKVWDFNGRCLHTMNAGLGRPVEISQVLLLRRSILVMGWERMLTVFRLHSFSQSFVEPSEWKGGAQHRGDVLCAAFQPPQTLVTGKEDSENFIAVTRLFFIPGRASVVAVTGGADLVSCGGSGTVRLWNTVHSRLVGQFTAHNSDLGSIVMTVSPCGKYLATASREGTIRTWDVQRYCLEPFEGVTEKPPRLLCSSRPHLDCVTHLETCIHGDKLLLLSASSDSSVALSFLPGEAVGLFGQVCRARTGHLSTDHGGQDDSSPRGVRSPPALSETHTDQNAEGRRECGESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YR53</accession>
    <name>A0A672YR53_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nuclear factor 7, ovary-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115428345</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YR53"/>
    <dbReference type="FunCoup" id="A0A672YR53">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007327.1">
        <property type="protein sequence ID" value="ENSSORP00005007064.1"/>
        <property type="gene ID" value="ENSSORG00005004068.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YR53"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="11"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="88"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="278"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="190"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="464" mass="53543" checksum="50B5500878B3AB67" modified="2020-06-17" version="1">MASRAEEDYCCPVCEDIFKDPVVLSCSHSFCKVCLQNWWKEKPKRECPVCKRRASREPPISMALKKLCESFLQQRNQRDQRNKTDPTDSESVCSLHSEKLKLFCLDHQQPVCVICQTSKIHNDHRFRPIDEAAQDLREELQNSLKPLKNKLKSLKKVKGEFNGTAEHIEVQARHTERQIKEQFKKLHQFLQEEEESRIRALREEEEQKSQRMKEKMEAVRREIAALSQTIRATEDELRAEHVSFLHKYKAAVERVQQRLLLEDPQLGSGALIDQAKHLGNLSFNIWNNMKDMVSYTPLVLDPNSAGPNLKVSEDLTEVKQKEEQKLPHNPERFRNCWVLGSESFTSGTHSWEVEVMDSKEWSLGVGTKSVQEGSLSQVWGIRFCKGKYLIWTSASGVTDLSVQIDSKRIRVSLDFNGGTLSFSDPDTNTHIYTFTHTFTGKILFPYLITTTMSSTRSKILLIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRZ1</accession>
    <name>A0A672YRZ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neuronal pentraxin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115425488</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029998965.1">
        <property type="nucleotide sequence ID" value="XM_030143105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YRZ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005007601.1">
        <property type="protein sequence ID" value="ENSSORP00005007325.1"/>
        <property type="gene ID" value="ENSSORG00005004190.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425488"/>
    <dbReference type="InParanoid" id="A0A672YRZ1"/>
    <dbReference type="OrthoDB" id="8871962at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF24">
        <property type="entry name" value="NEURONAL PENTRAXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025501330" evidence="9">
        <location>
            <begin position="22"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="10">
        <location>
            <begin position="217"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="122"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="423" mass="47350" checksum="B5CA519636771975" modified="2020-06-17" version="1">MDRSSWKLFLLSCLVFVESSAQDFGQTQFICTSVPKDMDLCTATMQNSGPAEDLKTTVMQLRETVLQQKETIMNQKETIRELTSKLSRCESQSLPAAEGPGGRRPGAKNTMGDVSRGTTDTLAQLGQTLQTLKQRLENLEQYSRGNNTVQANSLKDLLQNKIDDMEKQVLSRVNTLEETKPGPRNDTEQRNRVESTLTSLHHRITDLEKGKDNRPTDKFQLTFPLRTNYMYAKAKRSLPEMYSFSVCLWIKSNASPGVGTPFSYAVPGQANELVLIEWGNNPMEILINDKVAKLPFLINDGKWHHLCITWTTRDGMWEAFQDGVLRGNGENLAPYHPIKPEGVLVLGQEQDTLGGGFDATQAFVGELANLNIWNRKLSIAEIYNLATCNSKAPAGNVFSWTESNIEIFGGATKWTFEPCRSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUD7</accession>
    <name>A0A672YUD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YUD7"/>
    <dbReference type="Ensembl" id="ENSSORT00005008539.1">
        <property type="protein sequence ID" value="ENSSORP00005008251.1"/>
        <property type="gene ID" value="ENSSORG00005004592.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052208">
        <property type="entry name" value="DmX-like/RAVE_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950:SF12">
        <property type="entry name" value="DMX-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950">
        <property type="entry name" value="RABCONNECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="141" mass="15750" checksum="7F0C191596DBDD3E" modified="2020-06-17" version="1">MNLHQVLTGAVNPGDNCFSVGSVNNVPFTAYASGCDVVILGSDFERLQIIPGAKHGNIQVGCVDCSLRGGQVRAEERYSVPLCPEATLVYSRKCLCIQKYFCFFFVNYSFNLFLFHLISLITRRKKRICKTSTEITPTFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YW04</accession>
    <name>A0A672YW04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protein NEDD8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Neddylin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nedd8</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with AHR; interaction is direct. Interacts with NUB1; interaction is direct. Interacts with ESR1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YW04"/>
    <dbReference type="FunCoup" id="A0A672YW04">
        <property type="interactions" value="1812"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009172.1">
        <property type="protein sequence ID" value="ENSSORP00005008871.1"/>
        <property type="gene ID" value="ENSSORG00005004885.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YW04"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd01806">
        <property type="entry name" value="Ubl_NEDD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000023">
        <property type="entry name" value="NEDD8 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038738">
        <property type="entry name" value="Nedd8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="89" mass="10060" checksum="7883443A0CB39F61" modified="2020-06-17" version="1">MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIQGGSVLHLVLALRGGHRISLACSANQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWL7</accession>
    <name>A0A672YWL7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TRAF3-interacting JNK-activating modulator-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115422865</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030139467.1"/>
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        <property type="protein sequence ID" value="ENSSORP00005008910.1"/>
        <property type="gene ID" value="ENSSORG00005004913.1"/>
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    <dbReference type="InParanoid" id="A0A672YWL7"/>
    <dbReference type="OrthoDB" id="8886722at2759"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="entry name" value="CC1_T3JAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
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        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF21">
        <property type="entry name" value="TRAF3-INTERACTING JNK-ACTIVATING MODULATOR"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
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        <location>
            <begin position="67"/>
            <end position="117"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="191"/>
        </location>
    </feature>
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        <location>
            <begin position="371"/>
            <end position="436"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="463"/>
            <end position="518"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="588" mass="68458" checksum="B7126781443810C9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YY30</accession>
    <name>A0A672YY30_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Insulin-like growth factor-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">igfbp3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 3"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="286" mass="31097" checksum="52022CFC851293BF" modified="2020-06-17" version="1">MPALCVLCLAAALAAFTRLAGSVGPVIRCEPCDAGALLQCKPLPKDCAERVREPGCGCCMTCALGEGDACGVYTARCGSGLTCQHQPGESRPLQALLEGRGVCSTAASKKLCSILIPAQKQENTGNQVEEGIANVTATMTVLPGVATVKGGHSRMSVDTRPPLHSKLFQKDQNKKTQSYKVESVSGGAIMEMHNFSLENKREIEYGPCRREMESILNSLKISNVLIPGGFRIPNCDRKGFYKKKQCRPSKGRRRGYCWCVDKYGQPLPGFDRRERGETQCYNLESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2H4</accession>
    <name>A0A672Z2H4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SI:CH211-232M10.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613:SF9">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15125">
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            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
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        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
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        <location>
            <begin position="136"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="181" mass="19078" checksum="DAB21BAEA413928A" modified="2020-06-17" version="1">SGHASSLSGAAASWGLLSDTSACLPPPTVPYAVMEPVYRGLGRCACCFWLAVAFDIVGLLVLLLGVFVNVFFYDLLIYAGAIVIFLSLVWWVFWYSGNVEVPPAELEDDVGLLKRHKRGALGGLGGAVRRLSSRVSSGIRTSLRRNGAPAGTGRGSRAHGAPSGSAGTPVKKKYTVKKSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2I6</accession>
    <name>A0A672Z2I6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Atrophin-1-interacting protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">BAI1-associated protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Membrane-associated guanylate kinase inverted 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Membrane-associated guanylate kinase inverted 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">LOC115420523</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Plays a role in coupling actin fibers to cell junctions in endothelial cells, via its interaction with AMOTL2 and CDH5. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Cilium</location>
            <location evidence="5">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Photoreceptor inner segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Synapse</location>
            <location evidence="24">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005011195.1">
        <property type="protein sequence ID" value="ENSSORP00005010822.1"/>
        <property type="gene ID" value="ENSSORG00005005347.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2I6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031697">
        <property type="term" value="F:beta-1 adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
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        <property type="term" value="F:type II activin receptor binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
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        <property type="term" value="P:signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PDZ1_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06732">
        <property type="entry name" value="PDZ2_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06733">
        <property type="entry name" value="PDZ3_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06734">
        <property type="entry name" value="PDZ4_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000005">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000006">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000012">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000015">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000113">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000001">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000002">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000003">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF78">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16663">
        <property type="entry name" value="MAGI_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
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    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="22" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="16" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="20" id="KW-0770">Synapse</keyword>
    <keyword evidence="13" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="8" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="34">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="33">
        <location>
            <begin position="173"/>
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    </feature>
    <feature type="domain" description="WW" evidence="33">
        <location>
            <begin position="219"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="35">
        <location>
            <begin position="287"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="35">
        <location>
            <begin position="482"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="35">
        <location>
            <begin position="658"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="35">
        <location>
            <begin position="797"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="35">
        <location>
            <begin position="1025"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="39"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="424"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="575"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="744"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="887"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="1168"/>
            <end position="1241"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="1289"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="32">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="32">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="32">
        <location>
            <begin position="593"/>
            <end position="602"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="32">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="32">
        <location>
            <begin position="755"/>
            <end position="767"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="32">
        <location>
            <begin position="783"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="32">
        <location>
            <begin position="893"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="32">
        <location>
            <begin position="949"/>
            <end position="963"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="32">
        <location>
            <begin position="1183"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="32">
        <location>
            <begin position="1224"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="32">
        <location>
            <begin position="1313"/>
            <end position="1322"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004504"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00058771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00070827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00070829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00078448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00079516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00079517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00080410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1344" mass="144950" checksum="12A406F623429370" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4L2</accession>
    <name>A0A672Z4L2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">mRNA decay activator protein ZFP36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Zinc finger protein 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">zgc:162730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with the cytoplasmic CCR4-NOT deadenylase complex to trigger ARE-containing mRNA deadenylation and decay processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z4L2"/>
    <dbReference type="Ensembl" id="ENSSORT00005012161.1">
        <property type="protein sequence ID" value="ENSSORP00005011775.1"/>
        <property type="gene ID" value="ENSSORG00005006273.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z4L2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900153">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF157">
        <property type="entry name" value="MRNA DECAY ACTIVATOR PROTEIN ZFP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 5 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="353"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="388" mass="42208" checksum="C23853FC8053B6B5" modified="2020-06-17" version="1">MSEILSLSDFVMETNARLNRSASFFCPPSPPALSSHSLSSEHINNDDTSSPFWSSNIWSQAPISKPKQPPFRPDRSMSLTESSSTLLSSFGHMKNLDSFPSGPVPPLLSSNRYKTELCRGFQETGSCKYGSKCQFAHGEDELRGLYRHPKYKTEPCRTFYNFGYCPYGSRCHFIHEEKISGGPLPTGKFQSPRQTAPTVTSGQNPRHQLRQSVSFAGFLGSRSSPPPSFPASLIDPNLGFSRAPSVSPPPADLLSPVFSESLQREAAAFQFGQSRASTGDIHNIPLILEPKASRCVCGHGNKQSALNSRVFPSMEDGHYQDSSSMLLQGPGGHSGFMKSAGLQRFSSEDSLEDSYSSSSGGSSGTESPTFDGSSTKRLTVFERLSLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5C5</accession>
    <name>A0A672Z5C5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="7">Synaptosomal-associated protein 25</fullName>
                <shortName evidence="7">SNAP-25</shortName>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="6">Synaptosomal-associated protein</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115411198</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Synapse</location>
            <location evidence="7">Synaptosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z5C5"/>
    <dbReference type="Ensembl" id="ENSSORT00005012391.1">
        <property type="protein sequence ID" value="ENSSORP00005011999.1"/>
        <property type="gene ID" value="ENSSORG00005006364.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z5C5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070032">
        <property type="term" value="C:synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15885">
        <property type="entry name" value="SNARE_SNAP25C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000009">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000928">
        <property type="entry name" value="SNAP-25_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF5">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00835">
        <property type="entry name" value="SNAP-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 7" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="134"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="200" mass="22220" checksum="F8839E11621895C3" modified="2020-06-17" version="1">MADEADMRNELSDLQTRADQIADESLESTRRMLALVEEFYFTALMPLSSPEQLDRVEEGMNKVNADLKEAEKDLKDLGQCCGLICPCIKKIKGGGQAWGGNQDGVVNSQPGARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVGGIIGNLRHMALDMGQEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6F0</accession>
    <name>A0A672Z6F0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor HES-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Hairy and enhancer of split 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115418885</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z6F0"/>
    <dbReference type="FunCoup" id="A0A672Z6F0">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013309.1">
        <property type="protein sequence ID" value="ENSSORP00005012906.1"/>
        <property type="gene ID" value="ENSSORG00005006746.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z6F0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097150">
        <property type="term" value="P:neuronal stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11461">
        <property type="entry name" value="bHLH-O_HES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000033">
        <property type="entry name" value="Transcription factor HES-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="14">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="15">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="158" mass="18235" checksum="C80623A0F1E1F0C5" modified="2020-06-17" version="1">MAPTITAAMTNSEEHLTLTHKLRKPLVEKLRRERINSSIEQLKSLLGPEFLQQQPDSKMEKADILEMTVCFLRRLQQQHHVVDSAAVDQGYSRCAQKVVNFLSKEEVQTQSQRRLKNHFNKLQSSSDKKEEDFCLQSTVQTSITKDKSPVSSAPWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6Q0</accession>
    <name>A0A672Z6Q0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA demethylase ALKBH5</fullName>
            <ecNumber evidence="4">1.14.11.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alkylated DNA repair protein alkB homolog 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">alkbh5</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an N(6)-methyladenosine in mRNA + 2-oxoglutarate + O2 = an adenosine in mRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:49520"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="1.14.11.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:49521"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.53" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029984654.1">
        <property type="nucleotide sequence ID" value="XM_030128794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z6Q0"/>
    <dbReference type="SMR" id="A0A672Z6Q0"/>
    <dbReference type="FunCoup" id="A0A672Z6Q0">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012922.1">
        <property type="protein sequence ID" value="ENSSORP00005012525.1"/>
        <property type="gene ID" value="ENSSORG00005006577.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415246"/>
    <dbReference type="CTD" id="54890"/>
    <dbReference type="InParanoid" id="A0A672Z6Q0"/>
    <dbReference type="OrthoDB" id="271595at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990931">
        <property type="term" value="F:mRNA N6-methyladenosine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000002">
        <property type="entry name" value="RNA demethylase ALKBH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032860">
        <property type="entry name" value="ALKBH5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32074">
        <property type="entry name" value="RNA DEMETHYLASE ALKBH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32074:SF2">
        <property type="entry name" value="RNA DEMETHYLASE ALKBH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-ketoglutarate-dependent dioxygenase AlkB-like" evidence="16">
        <location>
            <begin position="90"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="272"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="41247" checksum="DF3944064EB25BEA" modified="2020-06-17" version="1">MAAGGYSDLREKLKSMTPHRDEYKNKYVDGTSNGSGKGRKRKYRESDDDDYEHSDDSSELREQEARRVRSSILQKSIFSPEECALIEEKIDEVVSKGEAGLYREHTVDRAPLRNKYFFGEGYTYGAQLEKRGPGQERLYRKGEVDEIPSWVHELVIKRLVSNGVIPEGFVNSAVINDYQPGGCIVSHVDPLHIFARPIVSVSFFSDSALCFGCRFQFKPIRVSEPVFVLPVRRGSVTVLSGYAADDITHCIRPQDIKERRAVIILRKTRPDAPRLDSDSALSSSPPERPVPLKAKRSHRKADPDAAHRPRVLEMDKEENRHPSLSRQRRHSISSENYWRRGQDYSKHRESSGRKVKMRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAX3</accession>
    <name>A0A672ZAX3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115423915</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZAX3"/>
    <dbReference type="Ensembl" id="ENSSORT00005014435.1">
        <property type="protein sequence ID" value="ENSSORP00005014015.1"/>
        <property type="gene ID" value="ENSSORG00005007183.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZAX3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="23"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="6">
        <location>
            <begin position="271"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="468" mass="52992" checksum="BC826CB474FEB048" modified="2020-06-17" version="1">VVSVLLVDMAAAGRVRSEDQFLCSICLDVFTDPVTIPCGHNFCKSCISEHWRVNVPYQCPMCKEFYIKPQLKINTFISEMVAQFRHEAQHEPNIYSSDQQAAKPGEVPCDVCSEPKLKKTELKKTEAEIQQMIQERRLKVQEIQRSVELSKNAADRKTAEGVEVFTALMDSVQRSLDQFIEEIQKKQRTTEKQAEVFIKELEQEICELKKRSTEVKQLSGSEDHLHFVQRFTCVKAAPSMKTWTKVSIRAPSYEGTVVRAVSELEDKLTKDMKKVVAEAELKRVQQYEVDLTLDPDTAHPTLILSDDGKQVHDGGVEKNLPDKPQRFSFCPCILAKQSFSSGRFYFEVQVKGKTGWILAVVRESINRKGEIQLSPRYGTWTICLTKGRDYKAFADPEVLLSVKAGLQKVGLFVDYEEGLVSFYDVDSSVLIYSFTGCCFRDKLLPFFCPCPSNGGLNSAPLIITLVTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZB11</accession>
    <name>A0A672ZB11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small integral membrane protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">smim8</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMIM8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984138.1">
        <property type="nucleotide sequence ID" value="XM_030128278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZB11"/>
    <dbReference type="FunCoup" id="A0A672ZB11">
        <property type="interactions" value="582"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014465.1">
        <property type="protein sequence ID" value="ENSSORP00005014044.1"/>
        <property type="gene ID" value="ENSSORG00005007197.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414916"/>
    <dbReference type="CTD" id="57150"/>
    <dbReference type="InParanoid" id="A0A672ZB11"/>
    <dbReference type="OrthoDB" id="1880105at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026686">
        <property type="entry name" value="UPF0708"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14274">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14274:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14937">
        <property type="entry name" value="DUF4500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="92" mass="10337" checksum="EB8C606F83A099FC" modified="2020-06-17" version="1">MADPEAGKQKADKGYRSPGLRGAQTSTLFRAVNPELFIKPNKPVMAFGLVAITLCVGYLGYLHAIKENDQQLYEAVDSEGQRYMRRKTSKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZB62</accession>
    <name>A0A672ZB62_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Si:ch73-290k24.5</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZB62"/>
    <dbReference type="Ensembl" id="ENSSORT00005014145.1">
        <property type="protein sequence ID" value="ENSSORP00005013732.1"/>
        <property type="gene ID" value="ENSSORG00005007068.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZB62"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd22109">
        <property type="entry name" value="F-box_FBXO41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057038">
        <property type="entry name" value="FBX41/ZN365_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052283">
        <property type="entry name" value="GenomicStab_NeuMorph_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15739:SF4">
        <property type="entry name" value="F-BOX ONLY PROTEIN 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15739">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23165">
        <property type="entry name" value="zf-C2H2_FBX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBX41/ZN365 C2H2-type zinc finger" evidence="5">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="303"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="111"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="608" mass="68080" checksum="454AEF274E0E71F0" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBC9</accession>
    <name>A0A672ZBC9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Src kinase-associated phosphoprotein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Src family-associated phosphoprotein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">skap2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in B-cell and macrophage adhesion processes. May play a role in src signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030012836.1">
        <property type="nucleotide sequence ID" value="XM_030156976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZBC9"/>
    <dbReference type="FunCoup" id="A0A672ZBC9">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014627.1">
        <property type="protein sequence ID" value="ENSSORP00005014205.1"/>
        <property type="gene ID" value="ENSSORG00005007278.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434854"/>
    <dbReference type="CTD" id="8935"/>
    <dbReference type="InParanoid" id="A0A672ZBC9"/>
    <dbReference type="OrthoDB" id="243840at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042113">
        <property type="term" value="P:B cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000194">
        <property type="entry name" value="Putative src kinase-associated phosphoprotein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000097">
        <property type="entry name" value="Putative src kinase-associated phosphoprotein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037781">
        <property type="entry name" value="SKAP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15129:SF2">
        <property type="entry name" value="SRC KINASE-ASSOCIATED PHOSPHOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15129">
        <property type="entry name" value="SRC-ASSOCIATED ADAPTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0075">B-cell activation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="105"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="283"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="52"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="345" mass="39512" checksum="CDC9818B20528F6D" modified="2020-06-17" version="1">MRAIPEEIITLISDLETFLSDGLKGENLSKRAKEKREAFIKRLKEVKLGFPQDFKDKSEEDSDDEEEVDSNNDGGSLQSERTDKDDEACEGTQQSPPVAALDLPSVFKAGYLEKRRKDHSFFGTEWQKRWCALSHQTFYYYGSEKDKQQKGEFSIEGYSVKMNNTLRKDSKKDCCFEISAPDKRVYQFCASSVKEAEEWVKQIDFVLRDMTGIIPEEDDEDQEMYDDVGAVQDNGDPEPIDDDIYEELPEDDIQTQAKPPPKVDLSSKPAPPVAVHSVDKSTDYQNYYQGLWDCIGDRPDELSFKRGDAIYILSKDYQNFGWWVGEKNGAIGIVPKDYLIELYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDG7</accession>
    <name>A0A672ZDG7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-crystallin A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cryba1a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDG7"/>
    <dbReference type="Ensembl" id="ENSSORT00005014932.1">
        <property type="protein sequence ID" value="ENSSORP00005014507.1"/>
        <property type="gene ID" value="ENSSORG00005007420.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDG7"/>
    <dbReference type="OrthoDB" id="8688215at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000004">
        <property type="entry name" value="Crystallin beta A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000002">
        <property type="entry name" value="Crystallin, beta B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF8">
        <property type="entry name" value="BETA-CRYSTALLIN A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="12"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="52"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="105"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="196" mass="23091" checksum="8A199C043BB9805A" modified="2020-06-17" version="1">MALNNPNPLGPWKITVYDQENFQGKRLEFTSSCQNIMECGVDNIRSLKVECGAWTGYEHSSFCGQQFVLERGEYPRWDSWSGSNAYHIERMMSFRPIYSAKHKDSKMVLFEKENFIGRQWEISDDYPSLQAMGWANNEIGSMLIQSGAWVCYQFPGYRGYQYIMECDRHDGEYKHYREWGSHAQSFQVQSLRRIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGM4</accession>
    <name>A0A672ZGM4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZGM4"/>
    <dbReference type="Ensembl" id="ENSSORT00005016127.1">
        <property type="protein sequence ID" value="ENSSORP00005015633.1"/>
        <property type="gene ID" value="ENSSORG00005007936.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGM4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000062">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003288">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001506">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="382"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="88"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="407" mass="47473" checksum="797D7C76C19E28FB" modified="2020-06-17" version="1">MAQKVQILRSLVEQRLTAAAEEIFGLFERTIAEYEEELCRTKEENQRQRQILDCVWNPRVLLHKTDVQQLLVVKEEVRPEHQEWNPIVDQEEPEPEPRHIKEEQEELWTSQEANITKFPSTPVPVKCEDDDDDEFNQTPTEEKKEEENRVDCGGSEPPSSFYPHRHLQIKINEQNEDSEETEDSNEDQILNHKPESKLPTNSSTEQMETETDGDDGGGSEPNIILVHMESVKGRTFESKSLLEEHMDTHTGQKLVGSSECEATFSQKKNTTQETNSPRDIMRKNNLSLSDLEWKNKLYHCSACSKTFCWKSKFEVHMRIHTGEKPFCCSVCGTRFKQKSSLRSHMKSHTGEKPFRCSLCQKSFRDRSEVKRHMIVHTGERPFSCVVCQKCFTRKDHLQTHMRIHTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKM5</accession>
    <name>A0A672ZKM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome c oxidase subunit 6C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytochrome c oxidase polypeptide VIc</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115419990</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A, COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the cytochrome c oxidase subunit 6c family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029990946.1">
        <property type="nucleotide sequence ID" value="XM_030135086.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029990949.1">
        <property type="nucleotide sequence ID" value="XM_030135089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZKM5"/>
    <dbReference type="FunCoup" id="A0A672ZKM5">
        <property type="interactions" value="1494"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018246.1">
        <property type="protein sequence ID" value="ENSSORP00005017726.1"/>
        <property type="gene ID" value="ENSSORG00005008828.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419990"/>
    <dbReference type="CTD" id="1345"/>
    <dbReference type="InParanoid" id="A0A672ZKM5"/>
    <dbReference type="OrthoDB" id="10051322at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22901">
        <property type="entry name" value="CcO_VIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.93.10">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIc/VIIs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051389">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034884">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc/VIIs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037169">
        <property type="entry name" value="Cytochrome_c_oxidase_VIc_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48416">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48416:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02937">
        <property type="entry name" value="COX6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81415">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="76" mass="8640" checksum="1CFFAEEF82A2C445" modified="2020-06-17" version="1">MSLPKPVMRGLLGRRLRFHLPVAFTLALTAAIAFKYTVTEPRKQAYADFYKQYDAVKEFNAMREAGVFQSARPTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLZ8</accession>
    <name>A0A672ZLZ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Galactosylceramide sulfotransferase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gal3st1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZLZ8"/>
    <dbReference type="Ensembl" id="ENSSORT00005017966.1">
        <property type="protein sequence ID" value="ENSSORP00005017448.1"/>
        <property type="gene ID" value="ENSSORG00005008717.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLZ8"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006682">
        <property type="term" value="P:galactosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000807">
        <property type="entry name" value="galactosylceramide sulfotransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF56">
        <property type="entry name" value="GALACTOSYLCERAMIDE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="421" mass="49691" checksum="41C09E6D590E3899" modified="2020-06-17" version="1">MSVPRRRWCSPAVRGLVFWVLLMNIMLVLYCLSNPTQPNIRSSQEDTCPIRMSKLWKENLTTPLQNTKPQCSPKVHIMFMKTHKTASSTILNILFRFGEKHQLKFAFPDGRNDFFYPSPFQCSQVKDYKPGDCFNIVCNHMRFDYQEVSKLLPPDAVYVTILRDPVDLFESSFHYYHQAVPLTWSIKSDNKLAEFLNSPQTFYRPEAFNSFYLKNLLFFDFGFDNNLESDNPRVTQALHTLSQRFHLVLMAEYFEESLILLKDTLCWTLEDVLYFKLNVRKSSSVSPLSLELRAKAMKWNGADWRLYRHFNATFWDKVEAFGQERMKREVNELRRRNGEMKDLCIEGGDAVEADQIQERRFVPWQPLGESSILGYNMKKDIDPKFRTMCQKMLTPEIQYLSELGVSLWLTRLWGWIKDTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQQ6</accession>
    <name>A0A672ZQQ6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZQQ6"/>
    <dbReference type="Ensembl" id="ENSSORT00005019277.1">
        <property type="protein sequence ID" value="ENSSORP00005018728.1"/>
        <property type="gene ID" value="ENSSORG00005009224.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQQ6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="85" mass="9085" checksum="E0E9A6C845AD74DE" modified="2020-06-17" version="1">MLPVQPAEESDCADEELGSISVGTSIGHGQILIRKLLSINGFASSSIVVGEVTALTKTEQSNAHLMNETKHWKQDKLLALAIPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZR43</accession>
    <name>A0A672ZR43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZR43"/>
    <dbReference type="FunCoup" id="A0A672ZR43">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019383.1">
        <property type="protein sequence ID" value="ENSSORP00005018832.1"/>
        <property type="gene ID" value="ENSSORG00005009268.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZR43"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025544884" description="Ig-like domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="115"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="230" mass="25959" checksum="EA89C781B76D5455" modified="2020-06-17" version="1">MCFHCSFVFSAMIWTTKVPQVISVTTVQLGESGIFNCLCSTDDQYFYLYKQPLGRLIETIATGVYSEITISKQFNNSRFSVTKVDAQYIFTIRGITKEDEALYFCENQTNQQNFISVEQNPPAASVQRGHSVSLQCSLLSENKQNPVQCPGEHNVYWFRAGSGGYRPGVIYTRKNKTEEADKRKCIYRLSKTVQDSSDSGTYYCAVATCGQILFGGGTKVERGTTFHHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZT42</accession>
    <name>A0A672ZT42_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adipocyte enhancer-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aortic carboxypeptidase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">aebp1a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZT42"/>
    <dbReference type="FunCoup" id="A0A672ZT42">
        <property type="interactions" value="649"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020126.1">
        <property type="protein sequence ID" value="ENSSORP00005019567.1"/>
        <property type="gene ID" value="ENSSORG00005009365.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZT42"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000068">
        <property type="entry name" value="Adipocyte enhancer-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000007">
        <property type="entry name" value="Carboxypeptidase X (M14 family), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000007">
        <property type="entry name" value="Carboxypeptidase X, M14 family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF48">
        <property type="entry name" value="ADIPOCYTE ENHANCER-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="F5/8 type C" evidence="14">
        <location>
            <begin position="53"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="15">
        <location>
            <begin position="231"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="775" mass="88596" checksum="C4553E194D2450F9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUP8</accession>
    <name>A0A672ZUP8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cannabinoid receptor 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cnr2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZUP8"/>
    <dbReference type="Ensembl" id="ENSSORT00005020526.1">
        <property type="protein sequence ID" value="ENSSORP00005019963.1"/>
        <property type="gene ID" value="ENSSORG00005009749.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZUP8"/>
    <dbReference type="OrthoDB" id="10011551at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004949">
        <property type="term" value="F:cannabinoid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002230">
        <property type="entry name" value="Cnbnoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00362">
        <property type="entry name" value="CANNABINOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="58"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="386" mass="42847" checksum="D44DE9A6E9A1AB61" modified="2020-06-17" version="1">MDAWEVPTCLGTTEAKDNVSSAVNKSCENLECYMVLMKAEKTAIGSICFLAGPITLLENTLVLLVIVATATLRQRPSYLFIGSLALADVFASCFFTTSFLDFHLFRRSDGPTAYLFKLGGVTMAFTSSVGSLLLTALDRYLCIHQASSYKILLTRRRALLSLLVLWSATTFISFLPLMGWRCPTGLVPPCSRLFPYINSGYLACWTSFILVLLTLILGAYALILWKAHRHEASMTSIQGAAGSGQARMRMDIRLARTFGLILLILVGCWLPALSFMIADVSMLLTHTQQRAFAFCSTLCLVNSAVNPLLYALRCRELRVALQQLFHRLCEIGRCKKLTEHLTPELASGHDISCTTISENEMHRTRTTTQLTTVSEIVTKQQKSIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVI6</accession>
    <name>A0A672ZVI6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tetraspanin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZVI6"/>
    <dbReference type="Ensembl" id="ENSSORT00005021621.1">
        <property type="protein sequence ID" value="ENSSORP00005020994.1"/>
        <property type="gene ID" value="ENSSORG00005010249.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZVI6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF216">
        <property type="entry name" value="TETRASPANIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="232" mass="25979" checksum="5FD19A23EB057987" modified="2020-06-17" version="1">MFDDSPSNKGFVVFLNLTHFYTKSVFFFFIITNLKQRLNFTNIPEGDSESFVGTLFVNVGFFCIAIGAVLVLMGLLGCCGAHKESKCLLLMFFSIVLIIFIAEVAAAVVALAEGLLRAWATPTLQNDYGSEPVVTKIWNTTMTELKCCGFTNYTDFMGSKFAKENGGSLPPSCCWTHSAPCSPEEALHICMYVCIILKMFSSFFCFFFKEKEGLHLKKDKYPSFKYPESSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWE3</accession>
    <name>A0A672ZWE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat flightless-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115433920</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LRRFIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZWE3"/>
    <dbReference type="Ensembl" id="ENSSORT00005021377.1">
        <property type="protein sequence ID" value="ENSSORP00005020752.1"/>
        <property type="gene ID" value="ENSSORG00005010118.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZWE3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019139">
        <property type="entry name" value="LRRFIP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19212">
        <property type="entry name" value="LEUCINE RICH REPEAT IN FLII INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19212:SF6">
        <property type="entry name" value="LEUCINE-RICH REPEAT FLIGHTLESS-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09738">
        <property type="entry name" value="LRRFIP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="224"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="429"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="580" mass="66311" checksum="3D1FD55F34B5BE51" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXV6</accession>
    <name>A0A672ZXV6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">guf1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030001998.1">
        <property type="nucleotide sequence ID" value="XM_030146138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZXV6"/>
    <dbReference type="FunCoup" id="A0A672ZXV6">
        <property type="interactions" value="1290"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021716.1">
        <property type="protein sequence ID" value="ENSSORP00005021088.1"/>
        <property type="gene ID" value="ENSSORG00005009947.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427552"/>
    <dbReference type="CTD" id="60558"/>
    <dbReference type="InParanoid" id="A0A672ZXV6"/>
    <dbReference type="OrthoDB" id="1074at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03699">
        <property type="entry name" value="EF4_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16260">
        <property type="entry name" value="EF4_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01890">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000078">
        <property type="entry name" value="Elongation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000015">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000007">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2570:FF:000001">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000004">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="entry name" value="Elongation factor 4, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512:SF7">
        <property type="entry name" value="TRANSLATION FACTOR GUF1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="TRANSLATION FACTOR GUF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="69"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="78"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="143"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="671" mass="75498" checksum="50556F659CD39748" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY60</accession>
    <name>A0A672ZY60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-14</fullName>
            <ecNumber evidence="7">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC115416113</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Early endosome membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="7"/>
    <dbReference type="RefSeq" id="XP_029985686.1">
        <property type="nucleotide sequence ID" value="XM_030129826.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029985687.1">
        <property type="nucleotide sequence ID" value="XM_030129827.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029985688.1">
        <property type="nucleotide sequence ID" value="XM_030129828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZY60"/>
    <dbReference type="Ensembl" id="ENSSORT00005022867.1">
        <property type="protein sequence ID" value="ENSSORP00005022210.1"/>
        <property type="gene ID" value="ENSSORG00005010831.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416113"/>
    <dbReference type="InParanoid" id="A0A672ZY60"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090382">
        <property type="term" value="P:phagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04122">
        <property type="entry name" value="Rab14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000344">
        <property type="entry name" value="Ras-related protein Rab-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030702">
        <property type="entry name" value="Rab14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00014884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="215" mass="23925" checksum="844AB74FCAB68E2D" modified="2020-06-17" version="1">MTGAPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMADCPHTIGVEFGTRIMEVHGQKVKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEEAFLEAAQRIYQNIQDGSLDLNAAESGVQHKPTAPQGGRLNADSQPVKDGCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY70</accession>
    <name>A0A672ZY70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">c1ql2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029980940.1">
        <property type="nucleotide sequence ID" value="XM_030125080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZY70"/>
    <dbReference type="FunCoup" id="A0A672ZY70">
        <property type="interactions" value="324"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022073.1">
        <property type="protein sequence ID" value="ENSSORP00005021427.1"/>
        <property type="gene ID" value="ENSSORG00005010483.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412518"/>
    <dbReference type="CTD" id="165257"/>
    <dbReference type="InParanoid" id="A0A672ZY70"/>
    <dbReference type="OrthoDB" id="10070467at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF69">
        <property type="entry name" value="COMPLEMENT C1Q-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025535331" description="C1q domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="8">
        <location>
            <begin position="117"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="250" mass="26536" checksum="78FAE2B0A4A5C26D" modified="2020-06-17" version="1">MVLALIIAIPLLVQTSKTDAHYEMMGTCRMICDPYNPKPSATALEVMQDLSAIPSPTFVQGTKGEPGRPGKPGPRGPPGEPGPPGPRGPPGDTGRTGFPGVVGTARTETSGELGSAIGGAKIAFYVGLKNPHEGYEVLRFDDVVTNLGNHYDPTTGKFTCQVSGIYYFTYHVLMRGGDGTSMWADLCKNGQVRASAIAQDADQNYDYASNSVVLHLDSGDEIYVKLDGGKAHGGNNNKYSTFSGFLLYPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A010</accession>
    <name>A0A673A010_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ADP-ribosylation factor-like protein 6-interacting protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">arl6ip4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ARL6IP4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029998754.1">
        <property type="nucleotide sequence ID" value="XM_030142894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A010"/>
    <dbReference type="Ensembl" id="ENSSORT00005022722.1">
        <property type="protein sequence ID" value="ENSSORP00005022067.1"/>
        <property type="gene ID" value="ENSSORG00005010774.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425375"/>
    <dbReference type="CTD" id="51329"/>
    <dbReference type="InParanoid" id="A0A673A010"/>
    <dbReference type="OrthoDB" id="48562at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019532">
        <property type="entry name" value="Nucl_RNA-splicing_assoc_SR-25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10500">
        <property type="entry name" value="SR-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="90"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="238" mass="26937" checksum="67B967FEFC968729" modified="2020-06-17" version="1">MERSRSPDRVSRDKKQVKKNKRRRSSSSSSSSSSSSSSSSRSRSRSSKKPSHKTKDVKTQRKKRRRSSSSSSSSSSSSSSSSSSSDEKRKKSKAKKKKNKKKKAKLKKQRKKEKKKKEKLKKKEKKKAAALLSAAPQKPASYLEVWQSEEGAVELGPVMTDEQKARLSTKRPLTKEEYEARQSVIRRVVDPETGRTRLVRGEGEIIEEIVSRERHKDINKQATKGDGSSFQRNLGLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A076</accession>
    <name>A0A673A076_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonuclease Oy-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnaset2l</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the RNase T2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A076"/>
    <dbReference type="Ensembl" id="ENSSORT00005023314.1">
        <property type="protein sequence ID" value="ENSSORP00005022649.1"/>
        <property type="gene ID" value="ENSSORG00005011013.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A076"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF85">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025330251" evidence="4">
        <location>
            <begin position="17"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="280" mass="32350" checksum="912F5E967F4F378A" modified="2020-06-17" version="1">MAPCSLLPLLLTLSTALQLHDLSTDHQDWDYKHGRRSQSPSCKWKCMQFTLQWPGGFCQSLYNESLCQIPPNINTWTIHGLWPQRVQRCCECWPLFLYDVQELEAELSERWPSLVKFKSSFQFWKDEWEKHGACAACVEELKGPRKYFQICLKLRQHFDIHRLLDDAGITPSCQRPYEVDEVHRVLTPLLGRKHELECVTDQQDRQVWFQVKTSLFPNLTIDCVHHRDADAMAAGSGRSYSRGHACPTDVPLYYLPIDHHHWGAEPVPDRPPPLGGGACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0P2</accession>
    <name>A0A673A0P2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A673A0P2"/>
    <dbReference type="Ensembl" id="ENSSORT00005023707.1">
        <property type="protein sequence ID" value="ENSSORP00005023035.1"/>
        <property type="gene ID" value="ENSSORG00005011163.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A0P2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="177"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="328"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="373" mass="41030" checksum="CB35A8448F8B0801" modified="2020-06-17" version="1">MATGVAASSFSALQEPEAIIVAPTRELINQIYMEARKFAFGTVVRPAVVYGGVNTAHQIRELSRGCNVLCGTPGRLLDIIARGKVGLSKVQYLVLDEADRMLDMGFEPDMRRLVGSPGMPSKENRQTLMFSATYPEDIQRMAADFLKTDYLFLAVGVVGGACSDVEQTFIQVTKFSKREQLMDFLKTTGTERTMVFVETKRQADFIATTLCQEKFPTTSIHGDREQREREQALADFRSGKCPVLVATSVAARGLDIPDVQHVVNYDLPSNIDDYVHRIGRTGRCGNIGRAVSFYDPDVDRELARALVSILSKAQQEVPSWLEECAFSGSGPCASSSPMKTFSATDSRKGRSFQENTVQNPPAAQAAAAEDDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3N7</accession>
    <name>A0A673A3N7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Multiple EGF-like-domains 6b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">megf6b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005024554.1">
        <property type="protein sequence ID" value="ENSSORP00005023854.1"/>
        <property type="gene ID" value="ENSSORG00005011480.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A3N7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000014">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000002">
        <property type="entry name" value="Multiple epidermal growth factor-like domains 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000037">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000041">
        <property type="entry name" value="Tyrosine protein kinase receptor tie-1, putative"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052108">
        <property type="entry name" value="MEGF/SIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035:SF137">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="91"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="447"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="491"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="714"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="753"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="902"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="990"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="1125"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="1271"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="1309"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="735"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="778"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="927"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="1015"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="1145"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="1296"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="1330"/>
            <end position="1339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1386" mass="147625" checksum="DD45D5C0096D4296" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4C9</accession>
    <name>A0A673A4C9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Synaptojanin-2-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial outer membrane protein 25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">synj2bp</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Binds (via the PDZ domain) to isoform 2A of SYNJ2 (via the unique motif in the C-terminus). Interacts (via C-terminus) with RALBP1. Interacts (via PDZ domain) with ACVR2A (via C-terminus) and ACVR2B (via C-terminus). Forms a ternary complex with ACVR2A and RALBP1. Interacts with MAPK12. Interacts with DLL1; enhances DLL1 protein stability, and promotes notch signaling in endothelial cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A4C9"/>
    <dbReference type="FunCoup" id="A0A673A4C9">
        <property type="interactions" value="982"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024171.1">
        <property type="protein sequence ID" value="ENSSORP00005023492.1"/>
        <property type="gene ID" value="ENSSORG00005011378.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4C9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06709">
        <property type="entry name" value="PDZ_SYNJ2BP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000161">
        <property type="entry name" value="Synaptojanin-2-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF51">
        <property type="entry name" value="DISKS LARGE 1 TUMOR SUPPRESSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="13"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="143" mass="15111" checksum="56A20D7FE876429E" modified="2020-06-17" version="1">MNGSLHSSSNVVDIKLKRGPAGLGFNIVGGVDQQYVVNDNGIFVSKIKEDGSAALDGRLQEGDKILAINGAMLDNRTHKAAVELFRSAGENVELKVLKKAPHLTNGPTGSQPEQEPSMSSLGILAVLAGAAAVIAFIYMRKHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4K6</accession>
    <name>A0A673A4K6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A4K6"/>
    <dbReference type="Ensembl" id="ENSSORT00005024292.1">
        <property type="protein sequence ID" value="ENSSORP00005023598.1"/>
        <property type="gene ID" value="ENSSORG00005011429.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4K6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="137" mass="15746" checksum="E8A70913AC2CF85D" modified="2020-06-17" version="1">TAITNTESPTEQCYYQCPKLHREAYGGDAMSRLAKQLVRTDRRLTVRMMAEQLSLNREPVRTILAEELGMRKECAKMVPKDLSDDQKEKFRFGINHRNSRTPKTKSHKAVPEVLQLNVAILLWRGHTCSSRTRSVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6U0</accession>
    <name>A0A673A6U0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A6U0"/>
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        <property type="protein sequence ID" value="ENSSORP00005025001.1"/>
        <property type="gene ID" value="ENSSORG00005012052.1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="15380" checksum="6BFE74450984180E" modified="2020-06-17" version="1">MHRCPLFCQVEVEEGAESVQLPFITTLDLPDDVRVRWFYYKDDEPKKLLHQACRSRTEMEINQVKTEDFSLTLKYPTDKDNYTYTCTIYSNENKILLKKRVELKVKGQYCAYTREVSGLLLYVYLFINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A940</accession>
    <name>A0A673A940_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Interferon-stimulated 20 kDa exonuclease-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">isg20l2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A940"/>
    <dbReference type="FunCoup" id="A0A673A940">
        <property type="interactions" value="1273"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025857.1">
        <property type="protein sequence ID" value="ENSSORP00005025102.1"/>
        <property type="gene ID" value="ENSSORG00005012085.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A940"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06149">
        <property type="entry name" value="ISG20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037433">
        <property type="entry name" value="ISG20_DEDDh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
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        <property type="entry name" value="Ribonucl_H"/>
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        <property type="entry name" value="RNaseH-like_sf"/>
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        <property type="entry name" value="INTERFERON-STIMULATED 20 KDA EXONUCLEASE-LIKE 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNase_T"/>
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    </dbReference>
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        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="7">
        <location>
            <begin position="138"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="88"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="322" mass="35317" checksum="C9BA35C706D7B524" modified="2020-06-17" version="1">MSDVWINASSVSDNTTLTTQTQKKLKKKEKWKKYLERKKNRKLQRTKAPGHSGSKPAPRPNGAKPTPPANWAYVSPFQPSASADTVTNHSSKPSTSTHSSCFLAVSGTKGDDPKPQSQSSWLPSSAGPSASSTFAPSKTLAIDCEMVGAGPKGHISQLARCSIVSYDGDVVYDKFISPSMPVTDYRTRWSGIRRSDLTGATPYAEARKEILKLLMGKVVVGHAIHNDFKVLGYAHPPALTRDTSRFPLLNQKAGFPENQAASLKRLTKAIFNRDIQTGRRGHSSVEDAKATMELYRAVEVEWERTPTSKTNNNRENTDTTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAB7</accession>
    <name>A0A673AAB7_9TELE</name>
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        <submittedName>
            <fullName evidence="12">Transcription factor E3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tfe3a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MiT/TFE family.</text>
    </comment>
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        <property type="gene ID" value="ENSSORG00005012583.1"/>
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="microphthalmia-associated transcription factor isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HLH_DNA-bd_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR031867">
        <property type="entry name" value="MiT/TFE_N"/>
    </dbReference>
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        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION FACTOR E3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3371"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="303"/>
            <end position="356"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
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        </location>
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        </location>
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            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <end position="147"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <begin position="507"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="526" mass="56558" checksum="C58FC5D5277ACBBF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAV0</accession>
    <name>A0A673AAV0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">La ribonucleoprotein 4B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">larp4b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AAV0"/>
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        <property type="gene ID" value="ENSSORG00005012425.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034900">
        <property type="entry name" value="LARP4B_RRM"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
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    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF43">
        <property type="entry name" value="LA-RELATED PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26088">
        <property type="entry name" value="RRM_LARP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="5">
        <location>
            <begin position="107"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="362"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="590"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="602"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="632"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="678" mass="74920" checksum="7878100EE7BCB872" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEM8</accession>
    <name>A0A673AEM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Carboxypeptidase E</fullName>
            <ecNumber evidence="17">3.4.17.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Carboxypeptidase H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Enkephalin convertase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Prohormone-processing carboxypeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">cpe</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Release of C-terminal arginine or lysine residues from polypeptides.</text>
            <dbReference type="EC" id="3.4.17.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.10" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A673AEM8"/>
    <dbReference type="FunCoup" id="A0A673AEM8">
        <property type="interactions" value="121"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028525.1">
        <property type="protein sequence ID" value="ENSSORP00005027729.1"/>
        <property type="gene ID" value="ENSSORG00005013236.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AEM8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03865">
        <property type="entry name" value="M14_CPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000004">
        <property type="entry name" value="Carboxypeptidase E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000013">
        <property type="entry name" value="carboxypeptidase N catalytic chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034232">
        <property type="entry name" value="M14_CPE_CPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF92">
        <property type="entry name" value="CARBOXYPEPTIDASE E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="23">
        <location>
            <begin position="32"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="22">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="456" mass="51638" checksum="D969E748008CDC00" modified="2020-06-17" version="1">MCACRVGLDAVETHGGCAQVREPGSDGEISFEYHRYEELRKALVSVWLQCPTITRIYTIGESFEGRELLVLEMSDNPGTHEPGEPEFKYIANMHGNEAVGRELLIYLAQYLCNQYQQGNETIIDLIHNTRIHLMPSMNPDGFEKAASQPGEIKDWFVGRSNAQGVDLNRNFPDLDRIIYINEREGGANNHLLQNMKKAVDENTKLAPETKAVVHWIMDIPFVLSANLHGGDVVANYPYDETRTGSTHEYSASPDDVMFKSLARAYSIYNPVMSDPHRPPCRKNDDDSSFKDGITNGGAWYSVPGGMQDFNYLSSNCFEITLELSCDKFPNEDTLKTYWEQNRNSLVNYIEQVHRGVKGFVRDLQGNPISNATISVEGIDHDITTAKDGDYWRLLAPGNYKVAASAPGYLTVIKKVAVPYSPATRVDFELESLMERKEEEREELMDWWKMMSETLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIY0</accession>
    <name>A0A673AIY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Growth arrest-specific 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">gas6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979093.1">
        <property type="nucleotide sequence ID" value="XM_030123233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AIY0"/>
    <dbReference type="Ensembl" id="ENSSORT00005030060.1">
        <property type="protein sequence ID" value="ENSSORP00005029229.1"/>
        <property type="gene ID" value="ENSSORG00005013845.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411221"/>
    <dbReference type="CTD" id="2621"/>
    <dbReference type="InParanoid" id="A0A673AIY0"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000146">
        <property type="entry name" value="Putative neurogenic locus notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000119">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000077">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051145">
        <property type="entry name" value="GAS-SHBG-PROS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040:SF14">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040">
        <property type="entry name" value="LAMININ G-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025350634" description="Growth arrest-specific 6" evidence="17">
        <location>
            <begin position="26"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="20">
        <location>
            <begin position="42"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="110"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="150"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="190"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="18">
        <location>
            <begin position="286"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="18">
        <location>
            <begin position="470"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="615"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="72172" checksum="8A09B7E6D2A41162" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALA2</accession>
    <name>A0A673ALA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin NUP188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nup188</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Nup188 family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A673ALA2">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029987.1">
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        <property type="gene ID" value="ENSSORG00005011458.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALA2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21094">
        <property type="entry name" value="Nup188_SH3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="11">
        <location>
            <begin position="7"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="12">
        <location>
            <begin position="501"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1223"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1476"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1659"/>
            <end position="1689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1665"/>
            <end position="1680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1706" mass="190694" checksum="206862D4E327B0AF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APA4</accession>
    <name>A0A673APA4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleolar MIF4G domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">SGD1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nom1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role in targeting PPP1CA to the nucleolus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">May interact with EIF4A1, EIF4A2 and EIF4A3. Interacts with PPP1CA and PPP1CC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029978979.1">
        <property type="nucleotide sequence ID" value="XM_030123119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673APA4"/>
    <dbReference type="FunCoup" id="A0A673APA4">
        <property type="interactions" value="1681"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031972.1">
        <property type="protein sequence ID" value="ENSSORP00005031104.1"/>
        <property type="gene ID" value="ENSSORG00005014836.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411158"/>
    <dbReference type="CTD" id="64434"/>
    <dbReference type="InParanoid" id="A0A673APA4"/>
    <dbReference type="OrthoDB" id="10260961at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000032">
        <property type="entry name" value="Nucleolar MIF4G domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF4">
        <property type="entry name" value="NUCLEOLAR MIF4G DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="9">
        <location>
            <begin position="640"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="207"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="240"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="847" mass="96624" checksum="0C7F28EF156D859C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ART3</accession>
    <name>A0A673ART3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Solute carrier family 2, facilitated glucose transporter member 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Glucose transporter type 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Glucose transporter type 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Solute carrier family 2, facilitated glucose transporter member 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">slc2a12</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Facilitative glucose transporter required for the development of the cardiovascular system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose(out) = D-glucose(in)</text>
            <dbReference type="Rhea" id="RHEA:60376"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984570.1">
        <property type="nucleotide sequence ID" value="XM_030128710.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029984571.1">
        <property type="nucleotide sequence ID" value="XM_030128711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ART3"/>
    <dbReference type="FunCoup" id="A0A673ART3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033252.1">
        <property type="protein sequence ID" value="ENSSORP00005032365.1"/>
        <property type="gene ID" value="ENSSORG00005015345.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415209"/>
    <dbReference type="CTD" id="154091"/>
    <dbReference type="InParanoid" id="A0A673ART3"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904659">
        <property type="term" value="P:D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000164">
        <property type="entry name" value="solute carrier family 2, facilitated glucose transporter member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050820">
        <property type="entry name" value="MFS_Sugar_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48023">
        <property type="entry name" value="D-XYLOSE-PROTON SYMPORTER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48023:SF2">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="509"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="19">
        <location>
            <begin position="37"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="577" mass="61743" checksum="7E6B9BA6FDC208E8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASW8</accession>
    <name>A0A673ASW8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Retinoblastoma-binding protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rbbp5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ASW8"/>
    <dbReference type="Ensembl" id="ENSSORT00005032377.1">
        <property type="protein sequence ID" value="ENSSORP00005031498.1"/>
        <property type="gene ID" value="ENSSORG00005014877.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASW8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000066">
        <property type="entry name" value="retinoblastoma-binding protein 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037850">
        <property type="entry name" value="RBBP5/Swd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040:SF1">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="403"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="339"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="476"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="513"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="558" mass="61536" checksum="ED050B528AEC4A4D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AT73</accession>
    <name>A0A673AT73_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA methyltransferase 10 homolog A</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA (guanine-9-)-methyltransferase domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA (guanine(9)-N(1))-methyltransferase TRMT10A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">trmt10a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="RefSeq" id="XP_030018771.1">
        <property type="nucleotide sequence ID" value="XM_030162911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AT73"/>
    <dbReference type="SMR" id="A0A673AT73"/>
    <dbReference type="FunCoup" id="A0A673AT73">
        <property type="interactions" value="1567"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032396.1">
        <property type="protein sequence ID" value="ENSSORP00005031517.1"/>
        <property type="gene ID" value="ENSSORG00005015004.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439018"/>
    <dbReference type="CTD" id="93587"/>
    <dbReference type="InParanoid" id="A0A673AT73"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18101">
        <property type="entry name" value="Trm10euk_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.30:FF:000001">
        <property type="entry name" value="tRNA methyltransferase 10 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016653">
        <property type="entry name" value="TRM10/TRM10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016323">
        <property type="entry name" value="tRNA_m1G_mtfrase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="85"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="275"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="35546" checksum="03CBC2FF014D4767" modified="2020-06-17" version="1">MAEEGTKSGSVCPQESENVETVNGNEEAADETLSKRQRKKLLKQQKWEEERELRKQRRKERKQQRRMQRQNHQEEEEGGDVQSAKKRPRREVTPSSLRLVVDCSFDDLMLIKDVRKLHKQIQRCYAENRRALHPVQFILSSLGGQLKQSMDEKDKGWVNWKDITIKTEHYSEVVAKEELVYLTSDSPNVLSELDQSKAYVIGGLVDHNHHKGITFERAKELGIDHAQLPLSSFVKMNSRKVLAVNHVFEIILAYLEKGSWQEAFFTVLPQRKGAVAVDQDETNTQDKEEDDSDSDSDADTQDQTEKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AY54</accession>
    <name>A0A673AY54_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Chromatin assembly factor 1 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Chromatin assembly factor I p60 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">chaf1b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
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    <dbReference type="FunCoup" id="A0A673AY54">
        <property type="interactions" value="1650"/>
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        <property type="protein sequence ID" value="ENSSORP00005033292.1"/>
        <property type="gene ID" value="ENSSORG00005015766.1"/>
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        <property type="component" value="Chromosome 21"/>
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    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:DNA repair"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006260">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:nucleosome assembly"/>
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        <property type="entry name" value="Chromatin assembly factor 1 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromatin assembly factor 1, subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
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    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="17">
        <location>
            <begin position="9"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="70"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="398"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="423"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="523"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="589"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="607" mass="66418" checksum="295061BB68E05B18" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0X2</accession>
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        <submittedName>
            <fullName evidence="8">RAD54 homolog B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rad54b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSSORP00005035836.1"/>
        <property type="gene ID" value="ENSSORG00005016884.1"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:helicase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
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        <property type="entry name" value="Helicase_C-like"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="SNF2-like_sf"/>
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    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
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        <property type="entry name" value="SNF2_N"/>
    </dbReference>
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        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
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    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025391084" evidence="5">
        <location>
            <begin position="23"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="83"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="414"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="649" mass="72926" checksum="0CF64592FD8A2C75" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0Z9</accession>
    <name>A0A673B0Z9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase TRIM39-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115426606</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1121" mass="122094" checksum="A0420443013CA567" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B193</accession>
    <name>A0A673B193_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B193"/>
    <dbReference type="Ensembl" id="ENSSORT00005036922.1">
        <property type="protein sequence ID" value="ENSSORP00005035966.1"/>
        <property type="gene ID" value="ENSSORG00005016971.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B193"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="106" mass="12463" checksum="9938C3693E6A8DE4" modified="2020-06-17" version="1">MEYADPVSDLLDPWGAFTTRLFRESCVFHKGNYVKDLSHLGRDLNRVIIIDNSPSVPVESWFDDTSDTELLDLLPFFERLSKVDDIYELLHRNISRIKSYIMRIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2X6</accession>
    <name>A0A673B2X6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospholipid phosphatase-related protein type 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lipid phosphate phosphatase-related protein type 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115411248</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B2X6"/>
    <dbReference type="FunCoup" id="A0A673B2X6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036659.1">
        <property type="protein sequence ID" value="ENSSORP00005035704.1"/>
        <property type="gene ID" value="ENSSORG00005016860.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2X6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000002">
        <property type="entry name" value="phospholipid phosphatase-related protein type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF17">
        <property type="entry name" value="PHOSPHOLIPID PHOSPHATASE-RELATED PROTEIN TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="10">
        <location>
            <begin position="104"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="301" mass="33294" checksum="CA24CC6134BE9FEB" modified="2020-06-17" version="1">MAGTVMLAYYFEYTDTFSVHVQGFFCYDTAYTKPYLGPEDSSAVPPVLLYTLVAGLPTLLITVTETVLFLVQYTSKEFDRSEKTVATGDCCYLNPLVRRTFRFLGVYTFGLFTVDIFVNAGQVVTGNLAPYFLTVCRPNYTSLGCHQALRYISHQEACTGNQDDILRARKTFPSKEAALSVYAALYLAMYVTCTVQAKGTRLAKPVLSLGLMCLAFLTGLNRVAEYRNHWSDVIAGFIIGTAIATFLVVCVVHNFKGKLLLSEETPAEQQSTSAMLNMGRVESPLEKYIASQVSTAQYSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5E7</accession>
    <name>A0A673B5E7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Voltage-dependent calcium channel gamma-7 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cacng7a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit (By similarity). Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization. Shows specificity only for GRIA1 and GRIA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B5E7"/>
    <dbReference type="FunCoup" id="A0A673B5E7">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037525.1">
        <property type="protein sequence ID" value="ENSSORP00005036559.1"/>
        <property type="gene ID" value="ENSSORG00005017235.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5E7"/>
    <dbReference type="OrthoDB" id="5917530at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016247">
        <property type="term" value="F:channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099590">
        <property type="term" value="P:neurotransmitter receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098943">
        <property type="term" value="P:neurotransmitter receptor transport, postsynaptic endosome to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051968">
        <property type="term" value="P:positive regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000003">
        <property type="entry name" value="Voltage-dependent calcium channel gamma-7 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051072">
        <property type="entry name" value="CACNG_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008371">
        <property type="entry name" value="VDCC_g7su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008368">
        <property type="entry name" value="VDCC_gsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107:SF12">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-7 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01792">
        <property type="entry name" value="VDCCGAMMA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01795">
        <property type="entry name" value="VDCCGAMMA7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="8" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="232"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="269"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="39313" checksum="366B4EF99C417DC8" modified="2020-06-17" version="1">MSSFSTRALTLLSSVFGACGLLLVGVAVSTDYWLLMEEGVVLQQNQTTEVKMALHSGLWRVCFVAGPEKGRCVASEYFTEPEIEITTENTANILKMVRTATPFPMVSLLFVFTAFVISNIGHIRPQRTILAFVSGIFFILSGLSLVVGLVLYISSINDEVMNRPREPEQFFHYHYGWSFAFAASSFLLKEGAGVMSVYLFMKRYAEEELYRPHPALYRPRMSDCSDYSGQFLHPDSWPPPQRGRSASDVSSDISIQLNQTPPPQPPPKGGSSQATPSSSGPSSAASYQLQPASSSSSSSYPHPLHPAATSTLPRVSHAHAHSQPHPHPSGPPPQSLPMAPPSAVPPPHYHTHMRMSASPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B627</accession>
    <name>A0A673B627_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 410</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cfap410</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in cilia formation and/or maintenance. Plays a role in the regulation of cell morphology and cytoskeletal organization. Involved in DNA damage repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Found in a complex with CFAP410, NEK1 and SPATA7. Interacts with NEK1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016945.1">
        <property type="nucleotide sequence ID" value="XM_030161085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B627"/>
    <dbReference type="Ensembl" id="ENSSORT00005036869.1">
        <property type="protein sequence ID" value="ENSSORP00005035913.1"/>
        <property type="gene ID" value="ENSSORG00005016936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437754"/>
    <dbReference type="CTD" id="755"/>
    <dbReference type="InParanoid" id="A0A673B627"/>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097733">
        <property type="term" value="C:photoreceptor cell cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000094">
        <property type="entry name" value="protein C21orf2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="262" mass="29518" checksum="9A84606FCCCA1A5E" modified="2020-06-17" version="1">MKLTRKLVLAKAKASDLESVKKLNCWGCNLTDISIFSQMPNIEVLTLSVNSITSLSPLAGCLSLCELYLRRNMIPSLSELSHLRPLTRLRVLWLAENPCCGTDPGQYRLTVLRCLPRLQKLDNQVVTEEEIALALMEGEEVTTPPGSIQNQLSTNGLPDAETENDPLNYNMEETNKIREQLGMKPLSRDKFPSLSSPSTRETKTSLRKSHTLDAVLLLLKDLEEEELRIVHTATQNRLQMYTLDSEMLNDSLQTQKTADIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B963</accession>
    <name>A0A673B963_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B963"/>
    <dbReference type="Ensembl" id="ENSSORT00005038120.1">
        <property type="protein sequence ID" value="ENSSORP00005037147.1"/>
        <property type="gene ID" value="ENSSORG00005017453.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B963"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF177">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="265" mass="30602" checksum="C3B9BF4D93FB2E3C" modified="2020-06-17" version="1">MNLYFSKKAFVRCMMHSCYSSLILFFIPWAAMQDTVRDDGKDIADYQSFALLAQTCLLIAVSIQMCLDTHYWTAINQFFVWGSLAAYFAVTFTMFSNGMFLIFTSIFPFIGTARNSLNQPNVWLTIFLATLLCVLPVVAFRFILIHYPVKLFPIQIYKVRKEGLPAPAPRRPPHRRISTRRSGYAFSHSQGYGDLVTSKRFLLRLPLKNAEAHAALTDDADGFDHFRLKKCNLLTKYHKVSAIFPHPSRILHNYLNQRFVNTFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BDZ3</accession>
    <name>A0A673BDZ3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Gamma-crystallin M3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115412278</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BDZ3"/>
    <dbReference type="Ensembl" id="ENSSORT00005039829.1">
        <property type="protein sequence ID" value="ENSSORP00005038832.1"/>
        <property type="gene ID" value="ENSSORG00005018150.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BDZ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF119">
        <property type="entry name" value="GAMMA-CRYSTALLIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="4"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="132"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="177" mass="21044" checksum="DEB21EC9B8DDDA3A" modified="2020-06-17" version="1">TMHGKIIFYEDRNFQGRSYECDSDCSDMSSHLSRCNSCRVKSGCFVVYDHPNYMGMQYFLRKGEYSDYQRMMGFSDCIRSCRMIPLHKGPYRLRIYEMENFGGQMNELVDDCDNIQSRFRISGCMSCHVMDGHWLMYEQPNYRGRMLYLRPGEYRSFSESGYDGIRFMSIRRITDSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJ37</accession>
    <name>A0A673BJ37_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BJ37"/>
    <dbReference type="Ensembl" id="ENSSORT00005041388.1">
        <property type="protein sequence ID" value="ENSSORP00005040348.1"/>
        <property type="gene ID" value="ENSSORG00005018838.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJ37"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="179" mass="20997" checksum="BB9673519087177E" modified="2020-06-17" version="1">PQCKPECKHQCKPQCKHQCKLQCKPHCKLHCKLQCKPQCKLHCKLQCKPQCKPQCKLQCKLQCKCKLQCKLQCKPQCKLACNLQCKLHCKPQCKLACKLQCKLQCKPQCKHQCKLQCKPQCKPQCKLQCKRQCKLQCKPQCKCQCKLQCKPQCKLQCKHQCKLQCKLQCKRQCKLQCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJI7</accession>
    <name>A0A673BJI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">trim45</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673BJI7"/>
    <dbReference type="Ensembl" id="ENSSORT00005041820.1">
        <property type="protein sequence ID" value="ENSSORP00005040767.1"/>
        <property type="gene ID" value="ENSSORG00005019029.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJI7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19809">
        <property type="entry name" value="Bbox1_TRIM45_C-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF291">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="32"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="12">
        <location>
            <begin position="175"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="361"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="549" mass="59350" checksum="4E169575FF381F5F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMR3</accession>
    <name>A0A673BMR3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BMR3"/>
    <dbReference type="Ensembl" id="ENSSORT00005044152.1">
        <property type="protein sequence ID" value="ENSSORP00005043060.1"/>
        <property type="gene ID" value="ENSSORG00005019926.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BMR3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="23667" checksum="AF9F8990CDD0F3F2" modified="2020-06-17" version="1">EDSPVLVSLLGVLVLGGAWLCSLATTLMSTWLTMCPDLLPTVTFELGLWEACVIHEGGTQGCQPHSSLLGLPPDIQLARILMCLSLAAGLLALLVAVPGMTGVKSCGERLRWKRALRTAGGALGLAAGVLGLAPVSYVAHLTVERFFDESVPEVMPRWEFGDAMFCGWTAGVLHLLAGTLLLASGRCAGGDDWSRPAPVSAGYPKTRTGTGTRTGTRTSTRSEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMR8</accession>
    <name>A0A673BMR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Serine/threonine-protein kinase Nek4</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Never in mitosis A-related kinase 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Serine/threonine-protein kinase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673BMR8"/>
    <dbReference type="Ensembl" id="ENSSORT00005042580.1">
        <property type="protein sequence ID" value="ENSSORP00005041513.1"/>
        <property type="gene ID" value="ENSSORG00005019307.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BMR8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000247">
        <property type="entry name" value="serine/threonine-protein kinase Nek4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF7">
        <property type="entry name" value="NIMA-RELATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 25 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0132">Cell division</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13 25 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 26" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00067731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="186" mass="21818" checksum="B19C973993618F43" modified="2020-06-17" version="1">MNNYIFIRVVGKGSYGEVNLVKHKTDRKQYVIKKLNLTTSTKRERRAAEQEAQLLSELRHPNIVTYRESWEGDDYQLYIVMGFCEGGDLYHRLKQQKGELLPERQVVEWFVQIAMALQYLHERNILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNHKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BN82</accession>
    <name>A0A673BN82_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pdk2a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673BN82"/>
    <dbReference type="FunCoup" id="A0A673BN82">
        <property type="interactions" value="1390"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043145.1">
        <property type="protein sequence ID" value="ENSSORP00005042072.1"/>
        <property type="gene ID" value="ENSSORG00005019446.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BN82"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF15">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE ISOZYME 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="235"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="49116" checksum="14AACA5BFBC94117" modified="2025-10-08" version="2">MRICLLLHVPKHIEHFSKFSPSPLSMKQFLDFGSINACEKTSFVFLRQELPVRLSNIMKEINLLPDKLLTTSSVQMVQSWYIQSLMEILEFLDQNPDDHKVLGEFVDALVTIRNRHNDVVPTMAQGIIEYKEAFPQDPVTNQNIQYFLDRFYMSRISIRMLINQHTLIFDGTTNPVHPNTIGSIDPHCQVTDVIQDAFHSAKMLCDQYYLRSPDLVIQEMNSKKKNHPITIVYVPSHLYHMLFELFKNAMRATIETHESSNNLPPIQVLVSLGGEDMSIRVSDKGGGVPFRRIENLFSYMYSTAPAPQIGEYTRPPLAGFGYGLPISRLYAKYFQGDLQLYSMEGHGTDAVIYLKALSTDSIERLPVYNKTALKNYKVSQEADDWCVPSKEPLDLSLVLQIFIPQSVQACLSELSYSDVMICCGRWTLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQE8</accession>
    <name>A0A673BQE8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:dkey-71h2.2</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BQE8"/>
    <dbReference type="Ensembl" id="ENSSORT00005043947.1">
        <property type="protein sequence ID" value="ENSSORP00005042857.1"/>
        <property type="gene ID" value="ENSSORG00005019839.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQE8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF81">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="1">
        <location>
            <begin position="44"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="151" mass="16852" checksum="C8E51E5B75FC0056" modified="2020-06-17" version="1">MDALKSAGRAIIKSPGVPRHTWGTSKHEKLPENWTDTKETLLEGMVFNVKYLGMTLVGQPKGEDMASAAIRRIVATARASAKKFRKVTLTVSPKGIIITDTETTDLIENVSIYSSFTKGRVHYITLPCLYSNLEQTVLRAFLRFSHLCGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRX6</accession>
    <name>A0A673BRX6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BRX6"/>
    <dbReference type="Ensembl" id="ENSSORT00005046692.1">
        <property type="protein sequence ID" value="ENSSORP00005045541.1"/>
        <property type="gene ID" value="ENSSORG00005020920.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRX6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047157">
        <property type="entry name" value="PHRF1/Atg35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618:SF20">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="38"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="116"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="24246" checksum="2468EA2F26037C83" modified="2020-06-17" version="1">MFSPVKKQKQKQYAFTLKEQSGSDNTANGKENAATDTCYICLSPFGNQTVGSVEKCQHTFCIECILQWSKTANTCPVDRTSFTLIHQRLCAGGAIQKKIKVRAPKRGFDEEEERNIVNCEKCGRNDRRNRMLECGRCDSGFHMFCLTPPLNVFPEGEWICPECELGPNNTGNLQIMLYFVFHLLFCVYHLLFSEGHYCFVLFFFAFYCKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BU43</accession>
    <name>A0A673BU43_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Matrix metallopeptidase 16b (membrane-inserted)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">mmp16b</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="19">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BU43"/>
    <dbReference type="FunCoup" id="A0A673BU43">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045794.1">
        <property type="protein sequence ID" value="ENSSORP00005044669.1"/>
        <property type="gene ID" value="ENSSORG00005020257.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BU43"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021805">
        <property type="entry name" value="Pept_M10A_metallopeptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF327">
        <property type="entry name" value="MATRIX METALLOPEPTIDASE 16B (MEMBRANE-INSERTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="552"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="24">
        <location>
            <begin position="100"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="317"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="366"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="413"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="463"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="270"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" evidence="17">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="19">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="20">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="595" mass="68376" checksum="0E3387CE1F96A932" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVD5</accession>
    <name>A0A673BVD5_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ugt5f1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673BVD5"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045645.1">
        <property type="protein sequence ID" value="ENSSORP00005044522.1"/>
        <property type="gene ID" value="ENSSORG00005020495.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVD5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000203">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF52">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY POLYPEPTIDE B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025710617" description="UDP-glucuronosyltransferase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="526" mass="59656" checksum="7909FCCA5128ABCA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BWF1</accession>
    <name>A0A673BWF1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein phosphatase 1, regulatory subunit 26</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BWF1"/>
    <dbReference type="Ensembl" id="ENSSORT00005048415.1">
        <property type="protein sequence ID" value="ENSSORP00005047241.1"/>
        <property type="gene ID" value="ENSSORG00005021605.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BWF1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026130">
        <property type="entry name" value="PPP1R26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031474">
        <property type="entry name" value="PPP1R26_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15724">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15724:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15740">
        <property type="entry name" value="PPP1R26_N"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 26 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 26 N-terminal" evidence="2">
        <location>
            <begin position="204"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 26 N-terminal" evidence="2">
        <location>
            <begin position="298"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 26 N-terminal" evidence="2">
        <location>
            <begin position="390"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="879"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="879"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="901"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="913"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="928" mass="103074" checksum="C2FBB39B32B34D66" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BWF2</accession>
    <name>A0A673BWF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ddr1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 24">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
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    <dbReference type="FunCoup" id="A0A673BWF2">
        <property type="interactions" value="614"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045591.1">
        <property type="protein sequence ID" value="ENSSORP00005044468.1"/>
        <property type="gene ID" value="ENSSORG00005019866.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BWF2"/>
    <dbReference type="OrthoDB" id="6071166at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
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        <property type="entry name" value="ADH_SHORT"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="193"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="157"/>
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        <location>
            <begin position="13"/>
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        <ligand>
            <name>NADP(+)</name>
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        <location>
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        <ligand>
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        <location>
            <position position="161"/>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000095"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000095-1"/>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU000363"/>
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        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046504.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="35415" checksum="F9BCC4DCD26B9029" modified="2020-06-17" version="1">MANSGQKVVLITGCSSGIGLRIAVTLAKDEKKRYHVIATMRDLKKKDKLVEAAGDAYGKTLMLLPLDVCSDESVKQCINNVKDRHIDILINNAGVGLLGPVESISIEEMKRVFETNFFGVVRMIKEVMPDMKKRRSGHIVVMSSVMGLQGVVFNDVYTASKFAMEGFCESMAVQLMKFNIRLSMIEPGPVHTEFETKMMEDVAKMEYPGADADTVRYFKDVYLPSSIDIFEAMGQTPEDIAKCTKKVIESNSPRFRNLTNSLYTPIVALKYADETGGLSVNTFYNLLFNFGPLMHITMNILKCLTCSCLRRRTISPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0R9</accession>
    <name>A0A673C0R9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Mitochondrial Rho GTPase</fullName>
            <ecNumber evidence="19">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">rhot2</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18 19">Atypical mitochondrial nucleoside-triphosphatase (NTPase) involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Can hydrolyze GTP, ATP and UTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>UTP + H2O = UDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64900"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:64901"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Mitochondrion outer membrane</location>
            <topology evidence="1 19">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="19"/>
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        <property type="interactions" value="1961"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047683.1">
        <property type="protein sequence ID" value="ENSSORP00005046522.1"/>
        <property type="gene ID" value="ENSSORG00005021088.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C0R9"/>
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        <property type="component" value="Chromosome 19"/>
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    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01893">
        <property type="entry name" value="Miro1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Miro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000011">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
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        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_hand_assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013567">
        <property type="entry name" value="EF_hand_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021181">
        <property type="entry name" value="Miro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08355">
        <property type="entry name" value="EF_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037488">
        <property type="entry name" value="Mt_Rho_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 19" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 19 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 19" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 19" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="22">
        <location>
            <begin position="4"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="306"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="22">
        <location>
            <begin position="418"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="618" mass="69642" checksum="827E2812E09A62D3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2V6</accession>
    <name>A0A673C2V6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor SYF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">syf2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing as component of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">May be part of a spliceosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SYF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029983310.1">
        <property type="nucleotide sequence ID" value="XM_030127450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C2V6"/>
    <dbReference type="FunCoup" id="A0A673C2V6">
        <property type="interactions" value="1553"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048441.1">
        <property type="protein sequence ID" value="ENSSORP00005047267.1"/>
        <property type="gene ID" value="ENSSORG00005021613.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414211"/>
    <dbReference type="CTD" id="25949"/>
    <dbReference type="InParanoid" id="A0A673C2V6"/>
    <dbReference type="OrthoDB" id="199717at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264">
        <property type="entry name" value="GCIP-INTERACTING PROTEIN P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="238" mass="28301" checksum="8C5FC0EF67B026FB" modified="2020-06-17" version="1">MASHSEESALATEEEPTETLASQKREARLRKFRELHFKRNEARKLNHQEVVEEDKRLKLPSNWEAKKARLEWELAEDEKKKECAARGENYDRVKLLEITADDAERWERKKKKKNPDTGFAGYAEAQFRQYQRLTKQIRPDMESYERQREECGEDFHPTSNSLIHGTHVPTKEGIDRMVEDVEKQIEKRSKYSRRRAYNDDADIDYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C336</accession>
    <name>A0A673C336_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">chst1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_029984166.1">
        <property type="nucleotide sequence ID" value="XM_030128306.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029984174.1">
        <property type="nucleotide sequence ID" value="XM_030128314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C336"/>
    <dbReference type="FunCoup" id="A0A673C336">
        <property type="interactions" value="581"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047899.1">
        <property type="protein sequence ID" value="ENSSORP00005046733.1"/>
        <property type="gene ID" value="ENSSORG00005021383.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414939"/>
    <dbReference type="CTD" id="8534"/>
    <dbReference type="InParanoid" id="A0A673C336"/>
    <dbReference type="OrthoDB" id="6138663at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045130">
        <property type="term" value="F:keratan sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001517">
        <property type="term" value="F:N-acetylglucosamine 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042339">
        <property type="term" value="P:keratan sulfate proteoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016469">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051135">
        <property type="entry name" value="Gal/GlcNAc/GalNAc_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704:SF36">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005883">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025416837" description="Sulfotransferase" evidence="12">
        <location>
            <begin position="23"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="13">
        <location>
            <begin position="79"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="48617" checksum="061514DDDEBA3B9B" modified="2020-06-17" version="1">MQCSWKAVILLALASIAIQYTAIRTLTSKPFQLCPLPSPQNCGLGGQETEPPFERGGAAGAGGGCDDYPYFSINATRKTHILVLATTRSGSSFVGQLLNQHQEVFYLFEPLYHVQTTLIPRLSHSRNAADRRVMLGASRDLLRSLYGCDLYFLESYIKPTPTNHTTDKLFRRGASRALCQQPVCDAFGPADVNVEEGDCVKKCATLNMTLATEACREKRHVAIKIVRVPEIGDLRALVEDPRLNIKVIQLVRDPRGILSSRIETFRDTYRLWRIWRATGRRPYNLDLSQLTVVCEDFLSSVSTGLSHPYWLKGKYMLVRYEDLARNPLLKTKEIYDYLGLPMDKNVEDWIHANTRGSNEPSAKHKFGTVRDSAANAESWRLKLSYEMVEYTQTVCQKVLHQLGYKAVKSVEELKNMSLSLVQDKTFVPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C762</accession>
    <name>A0A673C762_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSSORT00005049859.1">
        <property type="protein sequence ID" value="ENSSORP00005048667.1"/>
        <property type="gene ID" value="ENSSORG00005022147.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C762"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
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        <location>
            <begin position="2276"/>
            <end position="2364"/>
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        <source>
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        <source>
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        </source>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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            <taxon>Neoteleostei</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR037097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1119" mass="124234" checksum="E610D2970581F696" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9F0</accession>
    <name>A0A673C9F0_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">UDP-glucuronosyltransferase</fullName>
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        </recommendedName>
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    <gene>
        <name evidence="9" type="primary">LOC115425265</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030142670.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:glucuronosyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="UDP-GLUCURONOSYLTRANSFERASE 2A1"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025705577" description="UDP-glucuronosyltransferase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="486"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="532" mass="61120" checksum="FDE93A94CB269BCB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9P8</accession>
    <name>A0A673C9P8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Solute carrier family 25 member 34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">slc25a34</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989233.1">
        <property type="nucleotide sequence ID" value="XM_030133373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C9P8"/>
    <dbReference type="FunCoup" id="A0A673C9P8">
        <property type="interactions" value="407"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050703.1">
        <property type="protein sequence ID" value="ENSSORP00005049502.1"/>
        <property type="gene ID" value="ENSSORG00005022465.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418851"/>
    <dbReference type="CTD" id="284723"/>
    <dbReference type="InParanoid" id="A0A673C9P8"/>
    <dbReference type="OrthoDB" id="6703404at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000039">
        <property type="entry name" value="Solute carrier family 25 member 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051508">
        <property type="entry name" value="Mito_Carrier_Antiporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928">
        <property type="entry name" value="RE38146P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928:SF3">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="45"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="142"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="245"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="347" mass="37534" checksum="AEE7AFF56BF0924B" modified="2020-06-17" version="1">MTSTQLLETSPKQPAFASQPSHRMASTVSTVPSGVFGPSLPPPAVWPPLDFALGALSCCAACVFTNPLEVVKTRLQLQGELRARGSYTRHYRGVVQALLVVGRTDGLRGLQKGLSVGLIYQGVMNGVRLGSYSYCEALGITRFHGGSLLSGAAAGALGAFIASPAYLVKTHLQAQAVEAIAVGHQHNHQGVFDAFGTIYRRDGLIGLWRGVNGAVPRVMVGSAVQLGTFTSAKDWVSHTQWFKPNSWLTALIAAMISGVAVAISMTPFDVISTRLYNQPVDEFRRGRLYHGFSDCMLKVCQTEGVLGLYKGMGPVFVRLAPHTVLSMLFWDLMRQQVLKDNQSQRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGL8</accession>
    <name>A0A673CGL8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 70</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CGL8"/>
    <dbReference type="FunCoup" id="A0A673CGL8">
        <property type="interactions" value="562"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054550.1">
        <property type="protein sequence ID" value="ENSSORP00005053295.1"/>
        <property type="gene ID" value="ENSSORG00005023962.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGL8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009724">
        <property type="entry name" value="TMEM70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13281">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13281:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="148" mass="16918" checksum="79F2D672C379CD58" modified="2020-06-17" version="1">RWVKFFSYSTSGASLVLVPQILMKTGLGVQSFALQAAFCGVVGFFTFLTPVLLHLFTKGYVVRLYHNPDRDTYTAVTYNVFLAEKRTVFHQKQVRIPQVTKMFTTFYADKMGLLVNPDLFSIPQDFNHLMGYDKPFSFHTEVDPADKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CHJ2</accession>
    <name>A0A673CHJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleoside diphosphate kinase B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 12 13">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CHJ2"/>
    <dbReference type="Ensembl" id="ENSSORT00005054901.1">
        <property type="protein sequence ID" value="ENSSORP00005053645.1"/>
        <property type="gene ID" value="ENSSORG00005024107.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CHJ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04416">
        <property type="entry name" value="NDPk_TX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02948">
        <property type="entry name" value="TRX_NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
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        <property type="entry name" value="NME/NM23 FAMILY MEMBER 8"/>
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        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 6 ISOFORM X1"/>
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        <property type="entry name" value="NDK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
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        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
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    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="14">
        <location>
            <begin position="139"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="14">
        <location>
            <begin position="293"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="526" mass="59883" checksum="37C3C5E30633B6BF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CIV5</accession>
    <name>A0A673CIV5_9TELE</name>
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        <submittedName>
            <fullName evidence="11">Hydroxycarboxylic acid receptor 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115410434</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030122091.1"/>
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        <property type="nucleotide sequence ID" value="XM_030122093.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GeneID" id="115410434"/>
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    <dbReference type="OrthoDB" id="5969463at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="HCARs"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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            <end position="55"/>
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        <location>
            <begin position="67"/>
            <end position="87"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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        <location>
            <begin position="47"/>
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        </location>
    </feature>
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            <begin position="327"/>
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        </location>
    </feature>
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        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
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        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="40298" checksum="D9D8A65711079355" modified="2020-06-17" version="1">MLSHNVTMSLNSTRPGGVGGCPPVGIQLEGVVLPPVLTVDVVLGLLGNVVALWIFCVKLKSWNPNTLFLFNLVIADFMALVSLPLRIDALLRGHWVFGDGLCRINLFLMFSNRTASIALMTVVAVYRYFKVVHPHHRLNRLTKRQAVFVSASVWALVVAPRVPMLAYSHIKLPGNRTQCFFFTSYKEASRAIIILVSMHRILTVLEFAVPMLILLFCSVRIFSFLKQRQMGRPEKVRKAMRVCVAIVVVFVVCFLPTTVTTVGIWVIKSYRPWDCASFYTFTQLNIVSLGLNFLNSALDPVVYVFSSSMFRRALCGSLPRALRCKRDRDHHDKTASSSGSQSTTQQELKSIRADEGSEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CK22</accession>
    <name>A0A673CK22_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Small ribosomal subunit protein mS39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ptcd3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS39 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030001850.1">
        <property type="nucleotide sequence ID" value="XM_030145990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CK22"/>
    <dbReference type="FunCoup" id="A0A673CK22">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054256.1">
        <property type="protein sequence ID" value="ENSSORP00005053002.1"/>
        <property type="gene ID" value="ENSSORG00005023511.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427436"/>
    <dbReference type="CTD" id="55037"/>
    <dbReference type="InParanoid" id="A0A673CK22"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:ribosome"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:rRNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16276">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16276:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22330">
        <property type="entry name" value="Rib_mS39_PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="repeat" description="PPR" evidence="12">
        <location>
            <begin position="252"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="12">
        <location>
            <begin position="327"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="669" mass="75640" checksum="01243836939E925E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKQ1</accession>
    <name>A0A673CKQ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CKQ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005056071.1">
        <property type="protein sequence ID" value="ENSSORP00005054795.1"/>
        <property type="gene ID" value="ENSSORG00005024515.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKQ1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF28">
        <property type="entry name" value="ZINC FINGER PROTEIN 37-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="25"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="89" mass="10386" checksum="C9FBF88C0AEC81C8" modified="2020-06-17" version="1">MAQVSNHGKLLLQRLHQQREMDFLCDITIMVRDVEFRAHRNILAAFSKYFSSQADKGQEIATLDPDKVSRYALEKLLEFVYTGHMNLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CN43</accession>
    <name>A0A673CN43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pde6d</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Promotes the release of prenylated target proteins from cellular membranes. Modulates the activity of prenylated or palmitoylated Ras family members by regulating their subcellular location. Required for normal ciliary targeting of farnesylated target proteins, such as INPP5E. Modulates the subcellular location of target proteins by acting as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude. Stabilizes ARL3-GTP by decreasing the nucleotide dissociation rate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with the prenylated catalytic subunits of PDE6, an oligomer composed of two catalytic chains and two inhibitory chains; has no effect on enzyme activity but promotes the release of the prenylated enzyme from cell membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PDE6D/unc-119 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987889.1">
        <property type="nucleotide sequence ID" value="XM_030132029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CN43"/>
    <dbReference type="Ensembl" id="ENSSORT00005056945.1">
        <property type="protein sequence ID" value="ENSSORP00005055660.1"/>
        <property type="gene ID" value="ENSSORG00005024829.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417857"/>
    <dbReference type="CTD" id="5147"/>
    <dbReference type="InParanoid" id="A0A673CN43"/>
    <dbReference type="OrthoDB" id="10248777at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.40:FF:000002">
        <property type="entry name" value="Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.40">
        <property type="entry name" value="GMP phosphodiesterase, delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037036">
        <property type="entry name" value="PDED_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017287">
        <property type="entry name" value="Rhodop-sen_GMP-Pdiesterase_dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12976">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE DELTA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12976:SF0">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037825">
        <property type="entry name" value="GMP-Pdiesterase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0140">cGMP</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5" id="KW-0844">Vision</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="6">
        <location>
            <begin position="10"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="150" mass="17372" checksum="4B9FCAF1C33037D7" modified="2020-06-17" version="1">MSSDEDRAKEILKGFKLNWMNLRDAETGKVLWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSSEKLEKFRLEQKVFFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPANVLTGNVIIETKFYDDDLHVSTSRVRLFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CPH4</accession>
    <name>A0A673CPH4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ras-related protein Rab-33B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">zgc:101559</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CPH4"/>
    <dbReference type="Ensembl" id="ENSSORT00005058860.1">
        <property type="protein sequence ID" value="ENSSORP00005057541.1"/>
        <property type="gene ID" value="ENSSORG00005025500.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CPH4"/>
    <dbReference type="OrthoDB" id="10006973at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04115">
        <property type="entry name" value="Rab33B_Rab33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002685">
        <property type="entry name" value="RAB33A, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041822">
        <property type="entry name" value="Rab33A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="232" mass="26488" checksum="6FCF4544184E2C32" modified="2020-06-17" version="1">MAIHNKPGEEFDTVFSLNDSLELSSHHDYATAQARIFKIIVIGDSNVGKTCLTYRFCGGTFLKNPEATIGVDFRERTLELDGESIKLQIWDTAGQERFRKSMVEHYYRSVHAVIFVYDVTSLSSFESIPEWIEECSRHSVGPLVPRILVGNKCDLRDRREVPTSTAQCVADSYNFPLFETSAKDPTEKEHVDAIFLTLAYRLKSHKTLRLKQPSVSDVGQMWNQRENETCQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CYI9</accession>
    <name>A0A673CYI9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="32">Gephyrin</fullName>
            <ecNumber evidence="9">2.10.1.1</ecNumber>
            <ecNumber evidence="8">2.7.7.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">gphna</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="29">Also has a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.</text>
    </comment>
    <comment type="function">
        <text evidence="30">Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:35048"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>molybdopterin + ATP + H(+) = adenylyl-molybdopterin + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:31331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="EC" id="2.7.7.75"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:31332"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="31">Postsynaptic cell membrane</location>
            <topology evidence="31">Lipid-anchor</topology>
            <orientation evidence="31">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the C-terminal section; belongs to the MoeA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="9"/>
    <dbReference type="EC" id="2.7.7.75" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673CYI9"/>
    <dbReference type="Ensembl" id="ENSSORT00005058538.1">
        <property type="protein sequence ID" value="ENSSORP00005057233.1"/>
        <property type="gene ID" value="ENSSORG00005025012.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CYI9"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099634">
        <property type="term" value="C:postsynaptic specialization membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007529">
        <property type="term" value="P:establishment of synaptic specificity at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097112">
        <property type="term" value="P:gamma-aminobutyric acid receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072579">
        <property type="term" value="P:glycine receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.340.10:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000002">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.10:FF:000004">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF29">
        <property type="entry name" value="GEPHYRIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="24" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="25" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="20" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="23" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0770">Synapse</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="34">
        <location>
            <begin position="51"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="MoaB/Mog" evidence="34">
        <location>
            <begin position="614"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="216"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="33">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="33">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00050229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00051501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00055539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00059974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00060421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00073890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="848" mass="92404" checksum="4633A7072F0B8DB3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8Y6</accession>
    <name>A0A672Y8Y6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y8Y6"/>
    <dbReference type="Ensembl" id="ENSSORT00005003356.1">
        <property type="protein sequence ID" value="ENSSORP00005003258.1"/>
        <property type="gene ID" value="ENSSORG00005001988.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8Y6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="59" mass="7009" checksum="231798814BC8A999" modified="2020-06-17" version="1">MHTLCRLEHLVTMLLSSLSGGCRWIRTPERWFLVSIYAPYLIIPVMLLLTMLKYCTYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBV8</accession>
    <name>A0A672YBV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">AP3A hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Ectonucleotide pyrophosphatase/phosphodiesterase family member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">enpp4</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. Ap3A and Ap4A are diadenosine polyphosphates thought to induce proliferation of vascular smooth muscle cells. Acts as a procoagulant, mediating platelet aggregation at the site of nascent thrombus via release of ADP from Ap3A and activation of ADP receptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030012563.1">
        <property type="nucleotide sequence ID" value="XM_030156703.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030012572.1">
        <property type="nucleotide sequence ID" value="XM_030156712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YBV8"/>
    <dbReference type="FunCoup" id="A0A672YBV8">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001011.1">
        <property type="protein sequence ID" value="ENSSORP00005000980.1"/>
        <property type="gene ID" value="ENSSORG00005000596.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434650"/>
    <dbReference type="CTD" id="22875"/>
    <dbReference type="InParanoid" id="A0A672YBV8"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF79">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE ENPP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025342296" description="bis(5'-adenosyl)-triphosphatase" evidence="22">
        <location>
            <begin position="19"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="407"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="456" mass="51690" checksum="734DBFD9750CF838" modified="2020-06-17" version="1">MLLKILLLFLCGVRAVVSENSTAQPDPPPLLLVSFDGFRADYLQRFPMPNLKRLYSQGVLVKQLTNVFVTKTFPNHYSLVTGLYAESHGIVASNMYDPNSHKYFHVYNDSDPMWWSEAQPIWITAQDYGYKTASMMWPGSDVVNSNRTATHFFLYNDKVTFKQRLGNVTNWLVGDKEQAVKFAALYWEEPDRSGHKYGPDNTTAMREVLKEVDDNIGLLISELQRVDLWGKINVVVVSDHGMAQCSPERLIQLDDCLHPDNYTLVDHTPVAALIPKNNTKAVFDLLSNCHPHMTAYLKKSIPDKFHYRNNERIQPIILVADEGWTIIQRGNTLPRLGDHGYDNSLPSMHPFLAAAGPSFRQGYEMSSLQSVDVYPLLCRLLEVTPRPNNGTLSQARCMLAAETCWDVALMAGMVVGVLLALTVLTVLLRFATTRRRTLRPFRRIPIEDDDDDSLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCH5</accession>
    <name>A0A672YCH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rab27a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029986194.1">
        <property type="nucleotide sequence ID" value="XM_030130334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YCH5"/>
    <dbReference type="FunCoup" id="A0A672YCH5">
        <property type="interactions" value="377"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002736.1">
        <property type="protein sequence ID" value="ENSSORP00005002659.1"/>
        <property type="gene ID" value="ENSSORG00005001635.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416532"/>
    <dbReference type="CTD" id="5873"/>
    <dbReference type="InParanoid" id="A0A672YCH5"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04127">
        <property type="entry name" value="Rab27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000402">
        <property type="entry name" value="Ras-related protein Rab-27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041837">
        <property type="entry name" value="Rab27a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="220" mass="24755" checksum="69673532AC8CE1F7" modified="2020-06-17" version="1">MSDGEYDYLIKFLALGDSGVGKTSFLYQYTDGKFNSKFITTVGIDFREKRVIYKSTGPDGSSGKGQKIHMQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWMSQLQIHAYCENPDIVLCGNKCDLSDQRAVSEEEARELADKYGIPYFETSAANGQHVSQAVDVLLDLIMKRMERCVDKSWIPDGTVRANGPTNTDLSEGSERSKCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YEM5</accession>
    <name>A0A672YEM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Apolipoprotein C-I</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein C1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YEM5"/>
    <dbReference type="FunCoup" id="A0A672YEM5">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001911.1">
        <property type="protein sequence ID" value="ENSSORP00005001856.1"/>
        <property type="gene ID" value="ENSSORG00005001167.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YEM5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004859">
        <property type="term" value="F:phospholipase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032375">
        <property type="term" value="P:negative regulation of cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050995">
        <property type="term" value="P:negative regulation of lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010916">
        <property type="term" value="P:negative regulation of very-low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034447">
        <property type="term" value="P:very-low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.30">
        <property type="entry name" value="Apolipoprotein C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043081">
        <property type="entry name" value="ApoC-1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006781">
        <property type="entry name" value="ApoC-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16565">
        <property type="entry name" value="APOLIPOPROTEIN C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16565:SF2">
        <property type="entry name" value="APOLIPOPROTEIN C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04691">
        <property type="entry name" value="ApoC-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025430035" description="Apolipoprotein C-I" evidence="7">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9230" checksum="1F4B3C8518AE1563" modified="2020-06-17" version="1">MRLYLAVAVLVLAFVAYTEAQEETMEQKIANVGSQITEFGQQVAEQTKAALESFKNSETTQKIKSWADEYIEMIKNAVRPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF23</accession>
    <name>A0A672YF23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115429432</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Protein phosphatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed by the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). Interacts with subunits PPP6R1, PPP6R2 and PPP6R3. Interacts with subunit ANKRD28. Interacts with IGBP1. Interacts with MAP3K7. Interacts with NFKBIE. Interacts with TRIM14 and WRNIP1; these interactions positively regulate the RIG-I signaling pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A672YF23"/>
    <dbReference type="FunCoup" id="A0A672YF23">
        <property type="interactions" value="1962"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002285.1">
        <property type="protein sequence ID" value="ENSSORP00005002226.1"/>
        <property type="gene ID" value="ENSSORG00005001353.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YF23"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000005">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="111"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="306" mass="35300" checksum="24B1F06E9FDAE463" modified="2020-06-17" version="1">TMAPLDLDKYVEIARHCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQVPDTNYIFMGDFVDRGYYSLETFTHLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWRYCTKVFDMLTVAALIDEQVLCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPRGAGWLFGSKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNRREPKLFRAVPDSERVIPPRTTTPYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YH48</accession>
    <name>A0A672YH48_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YH48"/>
    <dbReference type="Ensembl" id="ENSSORT00005004018.1">
        <property type="protein sequence ID" value="ENSSORP00005003906.1"/>
        <property type="gene ID" value="ENSSORG00005002377.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YH48"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000621">
        <property type="entry name" value="FLT3-interacting zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000309">
        <property type="entry name" value="zinc finger X-chromosomal protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF390">
        <property type="entry name" value="ZINC FINGER PROTEIN 37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="517"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="103"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="550" mass="64168" checksum="78BB287A38923049" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIP5</accession>
    <name>A0A672YIP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YIP5"/>
    <dbReference type="Ensembl" id="ENSSORT00005004555.1">
        <property type="protein sequence ID" value="ENSSORP00005004423.1"/>
        <property type="gene ID" value="ENSSORG00005002685.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YIP5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000012">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000011">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000013">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF3">
        <property type="entry name" value="VOLTAGE-DEPENDENT P_Q-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="386"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="18">
        <location>
            <begin position="34"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="18">
        <location>
            <begin position="355"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="448" mass="51784" checksum="61675671BAD29504" modified="2020-06-17" version="1">GEGGPRPMPPFTSMFILTTTNPFRQACHYICTLRYFEMCVLLVIAMSSIALAAEDPVWPDSPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHPGSYFRDLWNILDFIVVSGALVAFAFTSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYLLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVKAQRREWKKYDFHYDNVAWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINARPLTRHMPQNKLSFQYRMWEFVVSPPFEYTIMAMIALNTVVLMMKYDGASDTYEDVLANLNIVFTSLFSMECVLKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGVSGMIFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIS0</accession>
    <name>A0A672YIS0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YIS0"/>
    <dbReference type="Ensembl" id="ENSSORT00005004586.1">
        <property type="protein sequence ID" value="ENSSORP00005004453.1"/>
        <property type="gene ID" value="ENSSORG00005002700.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YIS0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="86"/>
            <end position="126"/>
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    </feature>
    <feature type="coiled-coil region" evidence="5">
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            <begin position="127"/>
            <end position="161"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="233" mass="27047" checksum="2870A4B1B6A4E4B6" modified="2020-06-17" version="1">MNSGFLSCHVCSETFKDPVSLSCNHSFCSNCLKEFWEKTKNKNCPICKRKSSKDEPLVNFSLKELADSFVGRLNVGSSETEKGEKKVEVVCSKHQEEPKLFCKDENRAVCSICDFPHHREHTVVPVEEAVTEMKDQLKSDLKSLQEKKKKYEETQKTYDAIIQHLKKQVLFTENQIRAEFNKLHQFLREEEESRLAALREEEETHILGQIFSFLFICECFHLQPFVSVATFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJQ4</accession>
    <name>A0A672YJQ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YJQ4"/>
    <dbReference type="Ensembl" id="ENSSORT00005004945.1">
        <property type="protein sequence ID" value="ENSSORP00005004806.1"/>
        <property type="gene ID" value="ENSSORG00005002861.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJQ4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF114">
        <property type="entry name" value="PCDH2G3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
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    <sequence length="929" mass="101895" checksum="1218C0323215FB90" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSJ9</accession>
    <name>A0A672YSJ9_9TELE</name>
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        <recommendedName>
            <fullName evidence="4 12">Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1</fullName>
            <ecNumber evidence="3 12">2.1.1.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cap1 2'O-ribose methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">cmtr1</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">S-adenosyl-L-methionine-dependent methyltransferase that mediates RNA cap1 2'-O-ribose methylation to the 5'-cap structure of RNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA to produce m(7)GpppNmp (cap1).</text>
    </comment>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167609"/>
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        </reaction>
    </comment>
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        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
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    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 1"/>
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    </dbReference>
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    <keyword evidence="8 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="106"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="RrmJ-type SAM-dependent 2'-O-MTase" evidence="16">
        <location>
            <begin position="257"/>
            <end position="476"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
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        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="853" mass="97620" checksum="B68B0FDB976C0E69" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTE3</accession>
    <name>A0A672YTE3_9TELE</name>
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        <recommendedName>
            <fullName evidence="13">Adenosine deaminase RNA specific</fullName>
        </recommendedName>
    </protein>
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    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Cytoplasm</location>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YWB9"/>
    <dbReference type="Ensembl" id="ENSSORT00005009194.1">
        <property type="protein sequence ID" value="ENSSORP00005008893.1"/>
        <property type="gene ID" value="ENSSORG00005004900.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YWB9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025358671" evidence="1">
        <location>
            <begin position="21"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="212" mass="24945" checksum="E335C5DE93F6985A" modified="2020-06-17" version="1">MFSLCRFSLCRFSLCRFSLCRSSLCRFSLCRVSLCRFSLCRFSLCRFSLCRFSLCRFSLCRSSLCRFSLCRVSLCRFSLCRFSLCRFSLCRFSLCRFSLCRFSLCRSSLCRFSLCRFSLCRVSLCRFSLCRFSLCRFSLCRVSLCRFSLCRSSLCRFSLCRFSLCRVSLCRFSLCRFSLCRSSLCRFSLCRRPICPPLVHSLINRAVTLEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YY20</accession>
    <name>A0A672YY20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmg4</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029988344.1">
        <property type="nucleotide sequence ID" value="XM_030132484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YY20"/>
    <dbReference type="FunCoup" id="A0A672YY20">
        <property type="interactions" value="369"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009822.1">
        <property type="protein sequence ID" value="ENSSORP00005009503.1"/>
        <property type="gene ID" value="ENSSORG00005005205.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418130"/>
    <dbReference type="CTD" id="389362"/>
    <dbReference type="InParanoid" id="A0A672YY20"/>
    <dbReference type="OrthoDB" id="368507at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032157">
        <property type="entry name" value="PAC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33559">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33559:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16093">
        <property type="entry name" value="PAC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13823" checksum="2E81230FDB0F9779" modified="2020-06-17" version="1">MNETQNGAAFDPISVHNFSDKILEQVIHFHVMKLSGGFFLWIGSSPVMSNLAVSMSSKYDSMPLSTLVMGDPSNTAPNSLAQRLAKKTKKQVFVSYSLPVTDPNLSLLVENRIKKELELHPERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZ80</accession>
    <name>A0A672YZ80_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc-binding protein A33-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trim105</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZ80"/>
    <dbReference type="Ensembl" id="ENSSORT00005010376.1">
        <property type="protein sequence ID" value="ENSSORP00005010046.1"/>
        <property type="gene ID" value="ENSSORG00005005469.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZ80"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="228"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="161"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="49103" checksum="5D676584B698168D" modified="2020-06-17" version="1">MAAASPEGSLREDLTCVICCDLFREPVMLACMHHFCKPCITRYWRGTLSPVTCPQCRKEFSTKQFQTNYLVAAVVEKVRASTSDTQNLEKKVKEALESHRRRKEDIINIIRRDKDKMDTIKRVTADLQTRVCGEFRALRQILDDEETSALEQLKREQDEELMKVQQHLEPVEQALSELEENIRALEQASAATEKTILTELPQLSRPYAPMDFAPEFDTNAFSIKYMAPLQYIIWRKMFKSLKPGPTPLTFDINTAHPSISLSADQTMAVEYDAGVKHKSNNNKRFLQCVNVLASQGFHLGKHYWEVELGSKPKWDLGVAAETVDRHARIKLSPENGYWTIRLRNGNQYSAGTQPPSMLRLVSSPQRVGVFLECEERRVSFYNADDMTLIYQFTNGPRGKVFPFFSPCISDGDQKPHPIKLLHHPPVPLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z066</accession>
    <name>A0A672Z066_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G/T mismatch-specific thymine DNA glycosylase</fullName>
            <ecNumber evidence="15">3.2.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Thymine-DNA glycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115420744</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with AICDA and GADD45A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the uracil-DNA glycosylase (UDG) superfamily. TDG/mug family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.29" evidence="15"/>
    <dbReference type="RefSeq" id="XP_029992098.1">
        <property type="nucleotide sequence ID" value="XM_030136238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z066"/>
    <dbReference type="FunCoup" id="A0A672Z066">
        <property type="interactions" value="644"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010499.1">
        <property type="protein sequence ID" value="ENSSORP00005010168.1"/>
        <property type="gene ID" value="ENSSORG00005005516.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420744"/>
    <dbReference type="CTD" id="553789"/>
    <dbReference type="InParanoid" id="A0A672Z066"/>
    <dbReference type="OrthoDB" id="565731at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000002">
        <property type="entry name" value="G/T mismatch-specific thymine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF11">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="19">
        <location>
            <begin position="160"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="39"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="374"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="388"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="408"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061261"/>
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            <dbReference type="ARBA" id="ARBA00064519"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00066769"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00071248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="449" mass="49977" checksum="D9FE492B69648F31" modified="2020-06-17" version="1">MEEKLNGPLPVSPEYIQQWIQSTQQFHALQAQYSGLNQNHQYSYPAGESAMVNPQPQQEAADLTKPPPKKRGRPTQPKEPGAPKPPKVPKAPKPPKAPKPPKDPNAPKAKPGPKPKKNATEAQGDGKQEKIDESFKKVKRKVDRFKGMSEEEVMKKTLPDLLDYNLDYVIIGINPGLMAAFIGRWFPGPGNHFWKCLFLSGFTEEQLNHMHDTSLPVKYKMGFTNMVARATPGSKDLSSKELREGGKILVEKLKKFKPLIAVFNGKCIYEMFCRELFGKKPTKLEFGLQPHKIPDCDVALYLMPSSSARCAQFPRAQDKVHFYIKLRELRDQLKGVQKTSEVQEVDYTFNLQLAKEDAKRMAIKEEQYDPGYEDAYGGAYVEGGSENPPGQSQTNGHCTFSSEDTDRGPGASSSSSQPAPAVQIPDGQWMTQSFTDQIPDIGGGPRDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1Q8</accession>
    <name>A0A672Z1Q8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Solute carrier family 12 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">slc12a1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005010986.1">
        <property type="protein sequence ID" value="ENSSORP00005010644.1"/>
        <property type="gene ID" value="ENSSORG00005005756.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008511">
        <property type="term" value="F:sodium:potassium:chloride symporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055078">
        <property type="term" value="P:sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 12 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013612">
        <property type="entry name" value="AA_permease_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF93">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08403">
        <property type="entry name" value="AA_permease_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease N-terminal" evidence="18">
        <location>
            <begin position="40"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="16">
        <location>
            <begin position="133"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="16">
        <location>
            <begin position="401"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="17">
        <location>
            <begin position="575"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="973" mass="108861" checksum="FA0187FF59476095" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z396</accession>
    <name>A0A672Z396_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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</entry>
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</entry>
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    <sequence length="872" mass="96227" checksum="C5672396F51F1CA5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAS2</accession>
    <name>A0A672ZAS2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sterol 26-hydroxylase, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">si:ch211-113j14.1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZAS2"/>
    <dbReference type="FunCoup" id="A0A672ZAS2">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014854.1">
        <property type="protein sequence ID" value="ENSSORP00005014431.1"/>
        <property type="gene ID" value="ENSSORG00005007384.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZAS2"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042359">
        <property type="term" value="P:vitamin D metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF123">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 27 SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
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    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="528" mass="59595" checksum="B38543540D2747A3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBF0</accession>
    <name>A0A672ZBF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 39B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ttc39b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC39 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A672ZBF0"/>
    <dbReference type="Ensembl" id="ENSSORT00005015080.1">
        <property type="protein sequence ID" value="ENSSORP00005014651.1"/>
        <property type="gene ID" value="ENSSORG00005007154.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZBF0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF4">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025420831" description="Tetratricopeptide repeat protein 39B" evidence="7">
        <location>
            <begin position="22"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="591" mass="68324" checksum="CCA7B4D40F3E3775" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBL9</accession>
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        <submittedName>
            <fullName evidence="11">Si:ch211-207e14.4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">si:ch211-207e14.4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZBL9"/>
    <dbReference type="FunCoup" id="A0A672ZBL9">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014656.1">
        <property type="protein sequence ID" value="ENSSORP00005014234.1"/>
        <property type="gene ID" value="ENSSORG00005007279.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZBL9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031951">
        <property type="entry name" value="IL17R_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF17">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR D ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16742">
        <property type="entry name" value="IL17R_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
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            <end position="23"/>
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            <end position="639"/>
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            <end position="343"/>
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            <end position="582"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="639" mass="70852" checksum="09A583282E84E0B8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDD7</accession>
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        <recommendedName>
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        </recommendedName>
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        <name evidence="9" type="primary">oxr1a</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="4">May be involved in protection from oxidative damage.</text>
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        <subcellularLocation>
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        </subcellularLocation>
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        <text evidence="2">Belongs to the OXR1 family.</text>
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        <source>
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        <source>
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        </source>
    </evidence>
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</entry>
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        </recommendedName>
    </protein>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="72"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="172" mass="19126" checksum="DD71EB2B22A6C9F6" modified="2020-06-17" version="1">MALRRCWRFAGVAPRLFWRSPVLSGVSVPGGQSFVSNLVNSQFRCSELPVRRFSSAPSSTDVSYEELKQILAGRKAVVIDVREPWELREFGFIPGSINVPLGNVSTALQLPPEDFEEKYGGGMPRHTDHIVFTCLAGIRSKTALDTATSLGYKGVQHYPGGWQDWVKNEQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGB5</accession>
    <name>A0A672ZGB5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Actinodin1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZGB5"/>
    <dbReference type="FunCoup" id="A0A672ZGB5">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016824.1">
        <property type="protein sequence ID" value="ENSSORP00005016316.1"/>
        <property type="gene ID" value="ENSSORG00005008275.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGB5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="529" mass="60439" checksum="4C13A036D7218BD2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI35</accession>
    <name>A0A672ZI35_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.130</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Coenzyme Q biosynthesis protein 4 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">coq4</name>
        <name evidence="6" type="synonym">COQ4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion inner membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the COQ4 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.130" evidence="6"/>
    <dbReference type="RefSeq" id="XP_030004848.1">
        <property type="nucleotide sequence ID" value="XM_030148988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZI35"/>
    <dbReference type="FunCoup" id="A0A672ZI35">
        <property type="interactions" value="1255"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017293.1">
        <property type="protein sequence ID" value="ENSSORP00005016780.1"/>
        <property type="gene ID" value="ENSSORG00005008486.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429493"/>
    <dbReference type="CTD" id="51117"/>
    <dbReference type="InParanoid" id="A0A672ZI35"/>
    <dbReference type="OrthoDB" id="4249at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="276" mass="31353" checksum="2259A8A29C9932E3" modified="2020-06-17" version="1">MWLRASNPLRRLNRRQWSLLSKVVSVHQLHSSTSDFSQGSYDGLYPGHIPTTPLQKAVLAVGSGVAALQNPYRHDMVAVLGETTGHLALINLRDRMRNDPEGYEILIERPRIRLSTLDLSRMASLHDGSFGREYLRFLEVNHVTPDSRADVKFVDNEELAYVMQRYREVHDFMHTLLGMPTNMLGEVAVKWFEAAQTGLPMCSLGAVLGPLRLNIRQLQTLFTSLGPWALQNGRKARCVLSIFYEKRWEQSLEDLRQELNIDPPPIIPGPSNKRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJU0</accession>
    <name>A0A672ZJU0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZJU0"/>
    <dbReference type="Ensembl" id="ENSSORT00005017933.1">
        <property type="protein sequence ID" value="ENSSORP00005017415.1"/>
        <property type="gene ID" value="ENSSORG00005008708.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJU0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="105" mass="12185" checksum="2485B11C75F6EBB2" modified="2020-06-17" version="1">MQRCTMGIYVISKENGDPGHSDDVGIFVEGVLFLDIYLGSEAKACAVMHGVIYELNLAYPKELRHYYEFIQKELMQMDEEKLFQGPWPKQQNQCWTVGILQPHFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKB7</accession>
    <name>A0A672ZKB7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synembryn</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein Ric-8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ric8a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation. Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with some GDP-bound G alpha proteins. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synembryn family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZKB7"/>
    <dbReference type="FunCoup" id="A0A672ZKB7">
        <property type="interactions" value="1173"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018131.1">
        <property type="protein sequence ID" value="ENSSORP00005017611.1"/>
        <property type="gene ID" value="ENSSORG00005008783.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZKB7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000447">
        <property type="entry name" value="RIC8 guanine nucleotide exchange factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008376">
        <property type="entry name" value="Chaperone_Ric-8_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019318">
        <property type="entry name" value="Gua_nucleotide_exch_fac_Ric8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425:SF4">
        <property type="entry name" value="SYNEMBRYN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10165">
        <property type="entry name" value="Ric8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01802">
        <property type="entry name" value="SYNEMBRYN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="536" mass="60465" checksum="C065B95C1A2134B0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKY5</accession>
    <name>A0A672ZKY5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZKY5"/>
    <dbReference type="FunCoup" id="A0A672ZKY5">
        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018366.1">
        <property type="protein sequence ID" value="ENSSORP00005017841.1"/>
        <property type="gene ID" value="ENSSORG00005008878.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZKY5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030183">
        <property type="term" value="P:B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000140">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000366">
        <property type="entry name" value="receptor-type tyrosine-protein kinase FLT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001824">
        <property type="entry name" value="Tyr_kinase_rcpt_3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF356">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN KINASE FLT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00240">
        <property type="entry name" value="RECEPTOR_TYR_KIN_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="245"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="590" mass="67331" checksum="8C1A776BD8CE0728" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLJ8</accession>
    <name>A0A672ZLJ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">AE binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115421275</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the AEBP2/jing C2H2-type zinc-finger family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZLJ8"/>
    <dbReference type="FunCoup" id="A0A672ZLJ8">
        <property type="interactions" value="630"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018617.1">
        <property type="protein sequence ID" value="ENSSORP00005018076.1"/>
        <property type="gene ID" value="ENSSORG00005008972.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLJ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000471">
        <property type="entry name" value="Zinc finger protein AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052130">
        <property type="entry name" value="AEBP2/jing_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059034">
        <property type="entry name" value="SH3_AEBP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46541">
        <property type="entry name" value="ZINC FINGER PROTEIN AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46541:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26014">
        <property type="entry name" value="SH3_AEBP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="273"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="296"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="450" mass="48614" checksum="C8629263260CB344" modified="2020-06-17" version="1">MAAVEMAATVPEETPAVSVGNDTPPEETTEGDGKEKDKGPDEPETEEPGGSVEPKNEEANAEAGEETTGAAADKAPSEAVTEAVTDNDQEAAAAPEEAEPGEPKSEEPNNEPAMKEVKEEPGEERRCAGIDGEKPSGGGGEMSDKRRPSVEISSSDGEPLSRMDSEDSISSTLMEMESTVSSGRSTPAMMNGQSSAGSSGAKTVAYPCCWDLCPQYFNNSPDLAEHIRGIHVDGQRGGVFVCLWKGCKVYNTPSTSQSWLQRHMLTHSGDKPFKCVVGGCNASFASQGGLARHVPSHFSQQSSSKMSSQAKLKEESPSKAGLNKRKKLKNKRRCSLPRPHDFFDAQTMDAIRHRAICLNLATHIESVGNGHSVVFHSTVLARRKEGSGKVKVLLHWTPEDILPDVWVNETERSQLKTKVVHLSQLPQDTAMLLDPNIYRALPQKRLKRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZM34</accession>
    <name>A0A672ZM34_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HYDIN/VesB/CFA65-like Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZM34"/>
    <dbReference type="Ensembl" id="ENSSORT00005018808.1">
        <property type="protein sequence ID" value="ENSSORP00005018266.1"/>
        <property type="gene ID" value="ENSSORG00005009055.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZM34"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:axonemal central apparatus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033305">
        <property type="entry name" value="Hydin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053879">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23053">
        <property type="entry name" value="DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23053:SF0">
        <property type="entry name" value="HYDROCEPHALUS-INDUCING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22544">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PapD-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="6">
        <location>
            <begin position="179"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="6">
        <location>
            <begin position="421"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="648" mass="72277" checksum="4821AD2DE7909BCD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZN03</accession>
    <name>A0A672ZN03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ephb4a</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 is involved in the regulation of cell adhesion and cell migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. Involved in somitogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 30">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZN03"/>
    <dbReference type="FunCoup" id="A0A672ZN03">
        <property type="interactions" value="687"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019098.1">
        <property type="protein sequence ID" value="ENSSORP00005018551.1"/>
        <property type="gene ID" value="ENSSORG00005008774.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZN03"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048514">
        <property type="term" value="P:blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05065">
        <property type="entry name" value="PTKc_EphR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09554">
        <property type="entry name" value="SAM_EPH-B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000059">
        <property type="entry name" value="Ephrin type-A receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000015">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000071">
        <property type="entry name" value="Ephrin type-B receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000787">
        <property type="entry name" value="ephrin type-B receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000003">
        <property type="entry name" value="ephrin type-B receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037636">
        <property type="entry name" value="EPH-B4_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF19">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 23" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025627220" description="receptor protein-tyrosine kinase" evidence="23">
        <location>
            <begin position="25"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="542"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="27">
        <location>
            <begin position="26"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="327"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="438"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="619"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="25">
        <location>
            <begin position="912"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="744"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="625"/>
            <end position="633"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19 21">
        <location>
            <position position="651"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="70"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="985" mass="110139" checksum="D45C4BE76796EF3B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZPI5</accession>
    <name>A0A672ZPI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Retinoblastoma-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">107 kDa retinoblastoma-associated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">pRb1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">rbl1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. May act as a tumor suppressor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with AATF. Interacts with KDM5A. Interacts with KMT5B and KMT5C. Interacts with USP4. Interacts with RBBP9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029991053.1">
        <property type="nucleotide sequence ID" value="XM_030135193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZPI5"/>
    <dbReference type="Ensembl" id="ENSSORT00005018857.1">
        <property type="protein sequence ID" value="ENSSORP00005018313.1"/>
        <property type="gene ID" value="ENSSORG00005009008.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420040"/>
    <dbReference type="CTD" id="5933"/>
    <dbReference type="InParanoid" id="A0A672ZPI5"/>
    <dbReference type="OrthoDB" id="844594at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000035">
        <property type="entry name" value="RB transcriptional corepressor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.140:FF:000001">
        <property type="entry name" value="Retinoblastoma-like 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000082">
        <property type="entry name" value="retinoblastoma-like protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015030">
        <property type="entry name" value="RB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF20">
        <property type="entry name" value="RETINOBLASTOMA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="17">
        <location>
            <begin position="72"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="18">
        <location>
            <begin position="390"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="16">
        <location>
            <begin position="790"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein C-terminal" evidence="19">
        <location>
            <begin position="939"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="636"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="674"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1062" mass="118925" checksum="C379270AFCA5B948" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQ79</accession>
    <name>A0A672ZQ79_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZQ79"/>
    <dbReference type="Ensembl" id="ENSSORT00005019559.1">
        <property type="protein sequence ID" value="ENSSORP00005019004.1"/>
        <property type="gene ID" value="ENSSORG00005009364.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQ79"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF375">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="2">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="86" mass="9701" checksum="401ED9D55B92CA68" modified="2020-06-17" version="1">MSSEKNNCEVIELKLLTKPLTCCICCVFSDEGCGETLDPGPCRQYVVKWYYDPEANACAQFWYGGCQGNANKYETEAECRKSCVYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQQ8</accession>
    <name>A0A672ZQQ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondria-eating protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Spermatogenesis-associated protein 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">spata18</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MIEAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZQQ8"/>
    <dbReference type="FunCoup" id="A0A672ZQQ8">
        <property type="interactions" value="798"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020075.1">
        <property type="protein sequence ID" value="ENSSORP00005019516.1"/>
        <property type="gene ID" value="ENSSORG00005009593.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQQ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035694">
        <property type="term" value="P:mitochondrial protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035695">
        <property type="term" value="P:mitophagy by internal vacuole formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026169">
        <property type="entry name" value="MIEAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031981">
        <property type="entry name" value="MIEAP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21771:SF0">
        <property type="entry name" value="MITOCHONDRIA-EATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21771">
        <property type="entry name" value="MITOCHONDRIA-EATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16026">
        <property type="entry name" value="MIEAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondria-eating protein C-terminal" evidence="15">
        <location>
            <begin position="255"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="137"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="455"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="476" mass="54289" checksum="96EFA02CF92CF2E3" modified="2020-06-17" version="1">MADTLRRLTNTSSFSVLQDKLESWHQDYHVISCDQNLNRCCELIELTSKIQGQLFTILNLTAAEGGHYGGVDTLKTRLLPWLGTCFSMARPSVTNDTSLQLIQDSVEKDRRIRELSFSHENDMQKMETQLCSTRLQLDSVRSELADAQKELDDTKSKSATTLLATEDEIIQLKADLRSAHEQLDIYKRKLDSLDDYERQIRLLRDEVSYLSSEKAMLQERLVRSRSPSPLPRLSRSSSPIRSESPTRAQLTNSSRHARLVSRLSDLYAVERLEAQTLLRRYISDLEMVQKIIFIAVVESFRTAKLAYRQFKLRVRKTLSSSHFGPESLEDAAVDYIVRNLDLYDVQASVNDVINTMNVNPRISFPPEVDFVLISTLIRETCRVAFAMQTLDPPLDLAFASDGELYSDLRYRRSYDSEFSAPLVMYHVWPALMEGDAVIVKGEAVTRRGAMWSRSRSRSASPVRSRSLSPTRSLVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZRQ2</accession>
    <name>A0A672ZRQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ras-related GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rragca</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZRQ2"/>
    <dbReference type="Ensembl" id="ENSSORT00005020518.1">
        <property type="protein sequence ID" value="ENSSORP00005019955.1"/>
        <property type="gene ID" value="ENSSORG00005009742.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020520.1">
        <property type="protein sequence ID" value="ENSSORP00005019957.1"/>
        <property type="gene ID" value="ENSSORG00005009742.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26136at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11385">
        <property type="entry name" value="RagC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000001">
        <property type="entry name" value="Ras-related GTP-binding protein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000226">
        <property type="entry name" value="Ras-related GTP-binding protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039400">
        <property type="entry name" value="RagC/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF6">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="382" mass="43070" checksum="A03985CB39C66FBE" modified="2020-06-17" version="1">MSIQYEVEQLADSYGVADSFPKDFGYGEEEADIEDSPAPSDSKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKIYKDDISSSSFVNFQIWDFPGQVDFFDPTFDYEMIFRGTGALIFVIDAQDDYVEALGRLHLTVSRAYRVNPEINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADASLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYGLREDGSGSAYDKESMAIIKLNNTTVLYLKEVTKFLALVCILREESFERKGLIDYNFHCFRKAIHEVFEVGVSTPRPAGPQAVGSPCTKAVALNGTPRNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTU1</accession>
    <name>A0A672ZTU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Solute carrier family 41 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115419464</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SLC41A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZTU1"/>
    <dbReference type="Ensembl" id="ENSSORT00005021160.1">
        <property type="protein sequence ID" value="ENSSORP00005020586.1"/>
        <property type="gene ID" value="ENSSORG00005010011.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZTU1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000002">
        <property type="entry name" value="Solute carrier family 41, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF23">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="10">
        <location>
            <begin position="100"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="259" mass="28143" checksum="5C3E92E4A66D3A91" modified="2020-06-17" version="1">QIGVIVALGDLITLSLLAGISTGLYKELEFNNYANPLVCAAFVAMCPLWVLIARKIPSTREVLYSGWEPVIIAMAISSVGGLILDKTVTNPNFAGMAVFTPVINGVGGNLVAVQASRISTYLHMNGLPMGDPNPPRKCPTPCSSFFGSTVNSRSARVLFLLVAPGHLVFLYTINSLRGGHTTLTPIFITFYLAAALLQVMILLYLADWMVHWMWGRGMDPDNFSIPYLTALGDLLGTGFLALCFHMRWFIGDRDSNLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUR0</accession>
    <name>A0A672ZUR0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sult5a1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZUR0"/>
    <dbReference type="FunCoup" id="A0A672ZUR0">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021282.1">
        <property type="protein sequence ID" value="ENSSORP00005020704.1"/>
        <property type="gene ID" value="ENSSORG00005010065.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZUR0"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="35"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="303" mass="34842" checksum="29B6568CD4BFB76D" modified="2020-06-17" version="1">MARLDVVEIFHGISFPGHLHTQDSLQRAVEFPFQDTDIVIVSYPKSGTTWMQELVTLISSRGNPHLSQTVPNWTRAPWLEQFYLASVLEASTITPRVITTHLSQGLLSSALQVSKAKVIYVSRNPKDVAVSFYHFHKIANFLPDAGSFPEFLNKFLEGKLQYGCWFDHVKGWTSQTTSMDNLLHITYEEMSLDLRGVTEKVSSFLQCPLVDDEVNNCVAHCSFNSMKDNKMVNYTLVAEEIMDHKKGSFMRKGKIGDWKNMFTEEQNQYFDDVFKSKMQDCPLEFIWEDQDDKDTHRNPTEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZX13</accession>
    <name>A0A672ZX13_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zgc:161973</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZX13"/>
    <dbReference type="Ensembl" id="ENSSORT00005022468.1">
        <property type="protein sequence ID" value="ENSSORP00005021820.1"/>
        <property type="gene ID" value="ENSSORG00005010658.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZX13"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22093">
        <property type="entry name" value="F-box_FBXO15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46731">
        <property type="entry name" value="F-BOX ONLY PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46731:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="29"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="368" mass="43178" checksum="9FB675EF3B063129" modified="2020-06-17" version="1">MESTAKYEELKTPPTSIRPTILSVAAYWLLSLFRLPFEILTKILSYLDAPSLFCVSHVSKLFYQLANDEVIWHKIYLSEFGKNQMWLSKLVDDTAHRVKQVETENQRSFGQWKKMYFKTLAGQEVNKWRRQLRDISPYTGLPTQTEWVLRNLNVSWELMVCDRFGRKVTLEQTRVYYFETSVIVCWSNSSLPRHHHTTRIQLHGVRKGTLGNPRANWPNWHSLMLKSDLSTHPFYLIGKDRLIKLVFLSPGFIIGISRGRDTLAFIMVCLHFHRLVEKSLLGSPNCCVMSLTLLDEFQTPFWCVSAPVSTTVAKKCLSFDYSGKHFLIEYRDEDGHVKMMLVWLEEERQFFLIIFRVEEDFVCIFVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZX20</accession>
    <name>A0A672ZX20_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZX20"/>
    <dbReference type="Ensembl" id="ENSSORT00005021444.1">
        <property type="protein sequence ID" value="ENSSORP00005020818.1"/>
        <property type="gene ID" value="ENSSORG00005010171.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZX20"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001939">
        <property type="term" value="C:female pronucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042585">
        <property type="term" value="C:germinal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001940">
        <property type="term" value="C:male pronucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044727">
        <property type="term" value="P:epigenetic programing of male pronucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35678">
        <property type="entry name" value="PROTEIN STPG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35678:SF1">
        <property type="entry name" value="PROTEIN STPG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="150" mass="17387" checksum="A32B9C98C9883836" modified="2020-06-17" version="1">MFRKRALTITSKSVMSKMHRHYQTGLEMFLTTSQDTPVPGLYHVRDFIEESELNPVKKTYGFKGVGRRAATLGEYKGELLLPGAYHYTDSTQEVLKRHMTYSFKNCPRPDISTLEGRDKVGFRYRFMSGAKQSKTFTSRSEKTRKKMTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0L9</accession>
    <name>A0A673A0L9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neuropeptide FF receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">G-protein coupled receptor 147</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">RFamide-related peptide receptor OT7T022</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">npffr1l2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A0L9"/>
    <dbReference type="FunCoup" id="A0A673A0L9">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023445.1">
        <property type="protein sequence ID" value="ENSSORP00005022779.1"/>
        <property type="gene ID" value="ENSSORG00005011009.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A0L9"/>
    <dbReference type="OrthoDB" id="5975505at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000153">
        <property type="entry name" value="Neuropeptide FF receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005395">
        <property type="entry name" value="NPFF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF82">
        <property type="entry name" value="NEUROPEPTIDE FF RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01570">
        <property type="entry name" value="NPFFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="67"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="482" mass="54493" checksum="D96E459C004517E3" modified="2020-06-17" version="1">MEILDSEWKEELELEGSAAAQVLTNISQDGVANFTNITFFPYYQHTMYVAASYILAYFFIFVLCMVGNILVCLIVLGNHRMRTVTNLFILNLAISDLLVGIFCIPTTLVDNLITGWPFSNSVCKISGFVQGVSVSSSVFTLVAIAVERFRYIVYPLQPKPTIPVAKAAIMLIWVLAVAFMCPTVMALTVEKVPHHYMVYDDDFNNTLPLYTCFEDFTNGKMRKVYTAVLFTHIYLLPLTFITLMYGSIGVKLYLSVVANRELQLNDRSVQVRCRRGGQQMVSQKKIRVIKMLIMVALVFLLSWLPLWTLLMMADYAGLKRDQLDLLTSYIFPFAHWLAFSNSSINPIIYGYYNENFKRGFQAVCKSRPFCCFLQCDLWKRVVEWSRKGKSVQPCGGSDARANNSNPNDLSLGLQNQLRNDNKSTVTAELYRNVRVDSVVGQSDKSTSGCGLEMLAVNKKVNNTEESDRVTSMAVSVHQAWDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1Y5</accession>
    <name>A0A673A1Y5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ndufa6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Required for proper complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Mammalian complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029996365.1">
        <property type="nucleotide sequence ID" value="XM_030140505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A1Y5"/>
    <dbReference type="Ensembl" id="ENSSORT00005023971.1">
        <property type="protein sequence ID" value="ENSSORP00005023296.1"/>
        <property type="gene ID" value="ENSSORG00005011279.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423613"/>
    <dbReference type="CTD" id="4700"/>
    <dbReference type="InParanoid" id="A0A673A1Y5"/>
    <dbReference type="OrthoDB" id="14535at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20266">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045299">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016488">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006643">
        <property type="entry name" value="NDUA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="13">
        <location>
            <begin position="31"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="128" mass="15117" checksum="78BC753A63B14E1D" modified="2020-06-17" version="1">MASTAATRTAAAASRVVKPILSRDLDEAKRRVRELYRAWYREVPNTVAMFQLDISIRQGRNKVREMFDKNKHVTDPRVIDMLVIKGKMELQETIHVWKQKTHIMRYFHESEEPRSADFLSKFYKGHDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4D7</accession>
    <name>A0A673A4D7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phospholipid transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pltp</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029990963.1">
        <property type="nucleotide sequence ID" value="XM_030135103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A4D7"/>
    <dbReference type="FunCoup" id="A0A673A4D7">
        <property type="interactions" value="424"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024182.1">
        <property type="protein sequence ID" value="ENSSORP00005023503.1"/>
        <property type="gene ID" value="ENSSORG00005011382.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420002"/>
    <dbReference type="CTD" id="5360"/>
    <dbReference type="InParanoid" id="A0A673A4D7"/>
    <dbReference type="OrthoDB" id="8862579at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120017">
        <property type="term" value="F:ceramide transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990050">
        <property type="term" value="F:phosphatidic acid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904121">
        <property type="term" value="F:phosphatidylethanolamine transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035627">
        <property type="term" value="P:ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
        <property type="term" value="P:high-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00025">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.20.10:FF:000001">
        <property type="entry name" value="Phospholipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.10:FF:000001">
        <property type="entry name" value="phospholipid transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030675">
        <property type="entry name" value="BPI/LBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032942">
        <property type="entry name" value="BPI/LBP/Plunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001124">
        <property type="entry name" value="Lipid-bd_serum_glycop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017954">
        <property type="entry name" value="Lipid-bd_serum_glycop_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504">
        <property type="entry name" value="BACTERICIDAL PERMEABILITY-INCREASING BPI PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504:SF16">
        <property type="entry name" value="PHOSPHOLIPID TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02886">
        <property type="entry name" value="LBP_BPI_CETP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002417">
        <property type="entry name" value="Lipid_binding_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00329">
        <property type="entry name" value="BPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00400">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025468743" description="Phospholipid transfer protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="9">
        <location>
            <begin position="30"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein C-terminal" evidence="10">
        <location>
            <begin position="263"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="151"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002417-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="54372" checksum="AA3A259EC33AAA26" modified="2020-06-17" version="1">MASCLLTLLVLFIPLLPSIMAAEPAGCKIRITDKGLDMLKAETQRFVEEELSNISMPEMEGKEGRFQYTITNVKITEMNLTHVDLRFIPTVGLLFDVQNSSITLSFHRRILYWFFYDTGNISASADGVNINTVLNLIRDHEGRLKINNITCDARIEKMRAKFSGTLGKVYDFISGFLTSGMRFLLNQKICPALDHAALVHVNAMLDSIPVKTEVDGYIGIDYSLLSDPLVTSKSLDMEFRGMFFNLANENDILANSAVDPVITEYDRMVYLALSEFFFDSGMFSYYKAGIFQMDIVNEKMPKDLELLLRTTYFGALMMLNPALMDAPLALQLAVNSPPKTTIKTSGATVVVTAIVKVLVLPPGQPPVQLSSMTMENKFNAKVSMKGKRLAVHADLRRFKIFSNQSALESLALIPLQAPLKTMLQMSVVPLINNWTKRGVQIPLADGMDFIEEVVEYHNGYIVIGANLHFSKGLREMIEGSEVASNSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A9X3</accession>
    <name>A0A673A9X3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Brain-specific homeobox protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">bsx</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030009462.1">
        <property type="nucleotide sequence ID" value="XM_030153602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A9X3"/>
    <dbReference type="FunCoup" id="A0A673A9X3">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026783.1">
        <property type="protein sequence ID" value="ENSSORP00005026014.1"/>
        <property type="gene ID" value="ENSSORG00005012499.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432691"/>
    <dbReference type="CTD" id="390259"/>
    <dbReference type="InParanoid" id="A0A673A9X3"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000173">
        <property type="entry name" value="brain-specific homeobox protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF16">
        <property type="entry name" value="BRAIN-SPECIFIC HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="100"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="102"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="157"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="26363" checksum="C627BAC2F2F84786" modified="2020-06-17" version="1">MSMNYASAAPTQRSTSFFIEDILLHKPKPLREVIPSPFCTSLASRMPILEYGYPLMPTPILAPHPHHPLHKPEHHQYFFTSGMQMPALFQHHAELPGKHCRRRKARTVFSDSQLSGLEKRFEIQRYLSTPERVELATALSLSETQVKTWFQNRRMKHKKQLRKVQDERKTPGDVERSAENSSESELNDKSAEELKHGLEADSYMLEENDEDDDDVDIEDDICSPDHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADP6</accession>
    <name>A0A673ADP6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heme-binding protein soul2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ADP6"/>
    <dbReference type="Ensembl" id="ENSSORT00005028479.1">
        <property type="protein sequence ID" value="ENSSORP00005027685.1"/>
        <property type="gene ID" value="ENSSORG00005013230.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ADP6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF69">
        <property type="entry name" value="HEME-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025546354" description="Heme-binding protein soul2" evidence="2">
        <location>
            <begin position="18"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="20850" checksum="38D72E18F0B2A5F3" modified="2020-06-17" version="1">MGWLLFGAFLFLFGALAENQDRPTFEVLEKNEEYETRRYGVTVWITTKIPDESVPEITKSRDRLVKYCKKIEDNGYGTCSTNWPVLVNVTNDAEYSLSWFLVSDSDVSTLIPDPDVTVRHLLGGIAYVKKFGGTPSIESGKENAKTLREALANAGQTFNPHVYCGAGYDPFLSVTHHNEIWIFKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AF57</accession>
    <name>A0A673AF57_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 121-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tmem265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM121 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AF57"/>
    <dbReference type="Ensembl" id="ENSSORT00005027816.1">
        <property type="protein sequence ID" value="ENSSORP00005027032.1"/>
        <property type="gene ID" value="ENSSORG00005012937.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AF57"/>
    <dbReference type="OrthoDB" id="8936231at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032776">
        <property type="entry name" value="CECR6/TMEM121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042314">
        <property type="entry name" value="TMEM121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31046">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31046:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14997">
        <property type="entry name" value="CECR6_TMEM121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="302"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="38385" checksum="E67E5EBE9665A64C" modified="2020-06-17" version="1">MVPTPQVCVSTLVTVSTMAVVDLYLLEQSMLGARGIPGPSVWQCAAVLLGDVGFLLALRFVSAGVVSEARSPRRGFANALWFLFLSLLQLKLFFVCHNYRQERRPPDPLARKTLTLLLSICLPSLFLILTGADSMTPQRRKQEVRSRLLWVVVDLLDVLDLQAGLWEAQGGPMGGAVGAIEQKLPIWAEGLVFFYCYALLLLLPCVALTELGAAGLPGQRGARKEALYPWLSLVTINIFTLGLRGTGMLWYRDPRVSTVFLGKNLLALAVKLSSAWERNKQERSAGGTGAVAGAEPGDSTLPSQSSEQGEGQAQGKAPPSHYHTLSRSQSHTLSHVSLEPTETPLGPSFISHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AG99</accession>
    <name>A0A673AG99_9TELE</name>
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        <submittedName>
            <fullName evidence="6">Peptide YYa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115423532</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the NPY family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AG99"/>
    <dbReference type="Ensembl" id="ENSSORT00005029083.1">
        <property type="protein sequence ID" value="ENSSORP00005028274.1"/>
        <property type="gene ID" value="ENSSORG00005013512.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AG99"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:neuropeptide hormone activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:feeding behavior"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neuropeptide signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001955">
        <property type="entry name" value="Pancreatic_hormone-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020392">
        <property type="entry name" value="Pancreatic_hormone-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533">
        <property type="entry name" value="NEUROPEPTIDE Y/PANCREATIC HORMONE/PEPTIDE YY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEPTIDE YY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hormone_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00278">
        <property type="entry name" value="PANCHORMONE"/>
    </dbReference>
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        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00265">
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    </dbReference>
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        <property type="entry name" value="PANCREATIC_HORMONE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025506033" evidence="5">
        <location>
            <begin position="16"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="85" mass="9831" checksum="24D9680A8E2890B1" modified="2020-06-17" version="1">MAVMLKPWTVLVAVTLTPPKPENPGEDAPPEELAKYYTALRHYINLITRQRYGKRSAQEDVVAELLFGGDSNRDQRSRYDDSYMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJM1</accession>
    <name>A0A673AJM1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neuropilin (NRP) and tolloid (TLL)-like 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">neto2b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="AlphaFoldDB" id="A0A673AJM1"/>
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        <property type="protein sequence ID" value="ENSSORP00005028507.1"/>
        <property type="gene ID" value="ENSSORG00005013647.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJM1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000016">
        <property type="entry name" value="neuropilin and tolloid-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF26">
        <property type="entry name" value="NEUROPILIN AND TOLLOID-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="37"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="170"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="464"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="534" mass="60900" checksum="7CC967E7E2311C38" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AK67</accession>
    <name>A0A673AK67_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="primary">mettl24</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030129322.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005028732.1"/>
        <property type="gene ID" value="ENSSORG00005013738.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415658"/>
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    </dbReference>
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        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 24"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="3">
        <location>
            <begin position="127"/>
            <end position="331"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
            <end position="101"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="365" mass="41612" checksum="0B0C96C559A195FB" modified="2020-06-17" version="1">MGWDWLTRGLLLRVVVVLTAVGLCVHVYLEMRWQNPAAPLTPRRRIQSRGSRSKQTSAQTETQGHGDGKRRISYVRTLKKDRAGRKRDGGAEDPSPACCPPLHQHKKAPRWHIDLEPWASHSHSLEDEARRFLKYITTPQVSCLSPVGDEATHGGAEGAWAVCLDPKYSLSHRIHSKHCRVYSFGLGADDWSLEYSLARSGCDVHCFDPSLKQSHLQKAQMWLHRLSVDWRDPNPAMAQRQQSNTKKLATILNNFGHRQVDVLKADMESAEWKILENLILEGVVDSVGQLLLELHLHWAGFEVPGDNPSVVRYWFSLLKELERANFRLFHVHSDPTKPHLFLHKNVLNASSAYTLGWVNTQWKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AL37</accession>
    <name>A0A673AL37_9TELE</name>
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        <submittedName>
            <fullName evidence="7">DENN/MADD domain containing 4C</fullName>
        </submittedName>
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        <name evidence="7" type="primary">dennd4c</name>
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    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSSORT00005029897.1">
        <property type="protein sequence ID" value="ENSSORP00005029077.1"/>
        <property type="gene ID" value="ENSSORG00005009272.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="C-myc promoter-binding protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
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    <dbReference type="InterPro" id="IPR023341">
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        <property type="entry name" value="MVB12A/B"/>
    </dbReference>
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        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF17">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF2464"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="197"/>
        </location>
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            <end position="852"/>
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        <location>
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            <end position="936"/>
        </location>
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        </location>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51498"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1853" mass="204265" checksum="C87A8F5A3005FE22" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALU1</accession>
    <name>A0A673ALU1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Hemojuvelin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">hjv</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ALU1"/>
    <dbReference type="FunCoup" id="A0A673ALU1">
        <property type="interactions" value="35"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005029588.1"/>
        <property type="gene ID" value="ENSSORG00005014117.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALU1"/>
    <dbReference type="OrthoDB" id="10013795at2759"/>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010536">
        <property type="entry name" value="RGM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF3">
        <property type="entry name" value="HEMOJUVELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06535">
        <property type="entry name" value="RGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025656811" evidence="10">
        <location>
            <begin position="36"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule N-terminal" evidence="12">
        <location>
            <begin position="37"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="11">
        <location>
            <begin position="150"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="412" mass="44461" checksum="17D1340CF396AC22" modified="2020-06-17" version="1">MEPRAAATLHTELPWKRCLPLALLLVQLSFPRVEASCRILRCNSDFVAATLDLGSSSTGSAALSREAVNAGYCSALRSYATCTRRMARACRGDLAYHSAVQGIEDLLIQHRCPRAGPTAQPRPLPQGTLSGDSCLYEKGFIAREGHDLDYLHCSVFGDPHVRTFSHDFQTCGVKGAWPLIDNEYLYIQATSSPTGPGSNGTVLSKITIIFKSWRHCIDQQLYQAELDNVPAAFADGSVWSGERRGQHVLTVRTQSAGVHAEIRAAHIGTLLVVRQSGRSLSLAMRSPRAVVEAFAPEQDLQLCVWGCPASQRIDRLRPPSPSSPAVAVAAQAHCAALLPVRDVYYQACVFDLLTTGDLNASAAAVAAQQDAHTMIPDPESVHLLPIASAQPCHLRPDLLLLLGILGTLTRAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANU9</accession>
    <name>A0A673ANU9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Histone PARylation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115428870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HPF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030003957.1">
        <property type="nucleotide sequence ID" value="XM_030148097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ANU9"/>
    <dbReference type="FunCoup" id="A0A673ANU9">
        <property type="interactions" value="1229"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030935.1">
        <property type="protein sequence ID" value="ENSSORP00005030092.1"/>
        <property type="gene ID" value="ENSSORG00005014369.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428870"/>
    <dbReference type="CTD" id="54969"/>
    <dbReference type="InParanoid" id="A0A673ANU9"/>
    <dbReference type="OrthoDB" id="416496at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072572">
        <property type="term" value="F:poly-ADP-D-ribose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019361">
        <property type="entry name" value="HPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13386">
        <property type="entry name" value="HISTONE PARYLATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13386:SF1">
        <property type="entry name" value="HISTONE PARYLATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10228">
        <property type="entry name" value="HPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="352" mass="39975" checksum="5C2135EA27C3AEA1" modified="2020-06-17" version="1">MTGRAKRKPKSNQESDTGNGELKKARVDESKSVPLSEVDAEQRDEMKQLYQLQMPEDLYHFWDFCKEICPDNPRGALIDPLGLRLVGPFDILAGAHKNPKTPQPNFHLHWRYFYDPPEFQTIFQGSDDSQHHFGYYRDAPDLLPSFVGENDSKKGCVITQMGDNVFAAVYLYLLRKKKVKGSQKEGGKALESLETKLQDKAEALGLCLEQKTKGMKQRDKKVVTKTFHSAGIVVPVDKNDVGYRELPETDAGLKKICKAIAEAKNDEERVKAFGPLQEMVTFVQFANDECDYGMGYELGIDLFCYGSHYFHKVIKQLLPMAYTLLKRNLFGEILEAHLSSRSHDNLDQLSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APC9</accession>
    <name>A0A673APC9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673APC9"/>
    <dbReference type="Ensembl" id="ENSSORT00005031277.1">
        <property type="protein sequence ID" value="ENSSORP00005030423.1"/>
        <property type="gene ID" value="ENSSORG00005014509.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APC9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF22">
        <property type="entry name" value="PEPSIN A-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="8">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="64" mass="7268" checksum="6DC1DAA6B4887E86" modified="2020-06-17" version="1">MTINTFYSILFYSILFYSMVQLAYYGVISVGTPPQSFKVIFDTGSSNLWIPSIYCSSPACRTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AS83</accession>
    <name>A0A673AS83_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beclin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">becn1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endosome membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the beclin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030019383.1">
        <property type="nucleotide sequence ID" value="XM_030163523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AS83"/>
    <dbReference type="FunCoup" id="A0A673AS83">
        <property type="interactions" value="1791"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032163.1">
        <property type="protein sequence ID" value="ENSSORP00005031288.1"/>
        <property type="gene ID" value="ENSSORG00005014880.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439655"/>
    <dbReference type="CTD" id="8678"/>
    <dbReference type="InParanoid" id="A0A673AS83"/>
    <dbReference type="OrthoDB" id="20368at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.40:FF:000001">
        <property type="entry name" value="beclin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.40">
        <property type="entry name" value="Autophagy protein 6/Beclin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007243">
        <property type="entry name" value="Atg6/Beclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038274">
        <property type="entry name" value="Atg6/Beclin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041691">
        <property type="entry name" value="Atg6/beclin_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040455">
        <property type="entry name" value="Atg6_BARA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029318">
        <property type="entry name" value="BH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768">
        <property type="entry name" value="BECLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12768:SF4">
        <property type="entry name" value="BECLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04111">
        <property type="entry name" value="APG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17675">
        <property type="entry name" value="APG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15285">
        <property type="entry name" value="BH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="14 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beclin-1 BH3" evidence="22">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Atg6/beclin coiled-coil" evidence="23">
        <location>
            <begin position="132"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Atg6 BARA" evidence="21">
        <location>
            <begin position="261"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF15285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF17675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="447" mass="51185" checksum="D14C3C6F0D4972E0" modified="2020-06-17" version="1">MEGSKSSSTTMQVSFVCQRCCQPLKLDTSFNVLDRVTIQELIAPVVTVTPSKQPDSSEGETAPEETFVENKQDGVSRKYIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTSDLFDIMSGQTDVDHPLCEECTDTLLDHLDTQLNITENECQNYKQCLELLSHLQVEEEETLLAELQQLKEEEEALVQDLEAVEEQRAAVAQDLAQSRIHSQQLDTEELQYQKEYSEFKRQQLELDDELKSVDNQMRYCQIQLDRLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLRFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGDTGFCLPYRMDVDKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVTSQFYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATT6</accession>
    <name>A0A673ATT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA-queuosine alpha-mannosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.110</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glycosyltransferase-like domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Glycosyltransferase that specifically catalyzes mannosylation of cytoplasmic tRNA(Asp) modified with queuosine at position 34 (queuosine(34)). Mannosylates the cyclopentene moiety of queuosine(34) in tRNA(Asp) to form mannosyl-queuosine(34). Mannosylation of queuosine(34) in tRNA(Asp) is required to slow-down elongation at cognate codons, GAC and GAU, thereby regulating protein translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>queuosine(34) in tRNA(Asp) + GDP-alpha-D-mannose = O-4''-alpha-D-mannosylqueuosine(34) in tRNA(Asp) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12885"/>
            <dbReference type="Rhea" id="RHEA-COMP:18572"/>
            <dbReference type="Rhea" id="RHEA-COMP:18581"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:194431"/>
            <dbReference type="ChEBI" id="CHEBI:194442"/>
            <dbReference type="EC" id="2.4.1.110"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:12886"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.110" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673ATT6"/>
    <dbReference type="FunCoup" id="A0A673ATT6">
        <property type="interactions" value="344"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033910.1">
        <property type="protein sequence ID" value="ENSSORP00005033006.1"/>
        <property type="gene ID" value="ENSSORG00005015635.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATT6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016438">
        <property type="term" value="F:tRNA-queuosine(34) beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01635">
        <property type="entry name" value="Glycosyltransferase_GTB-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051862">
        <property type="entry name" value="GT-like_domain_containing_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022701">
        <property type="entry name" value="QTMAN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13615">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13615:SF3">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12038">
        <property type="entry name" value="QTMAN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA-queuosine alpha-mannosyltransferase N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="9">
        <location>
            <begin position="255"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="426" mass="49161" checksum="A4DFF810D5633FD6" modified="2020-06-17" version="1">MNLHSYSLVLLVEAFYGGSHKQLIDLLKENIDICCTFTLPAKKWHWRARTAALHFMEAIPPRPSYRVLFCSSVLNLCELVALRPDLSHLKKILYFHENQLVYPVRKDQERDFQYGYNQILSCLVADVVAFNSAFNMESFLSSISSFMKKIPDHRPKGLDQLIRKKSVVLYFPLQLPDVSRSVDRQSNTDRYIQTDTVCLLVGLTRFKDLFPVETLILFNQTEPYRCEEQDPHGSSPDPRVDLRLAEDQVRPLHIVWPHRWEHDKNPELLFSTLLKLKDRGLDFRLSVLGETFTDVPAVFSEARQQLDSHIEHWGFLESKDQYLRTLSQADVVISTAKHEFFGVAMLEAVHCGCYPLCPNALVYPEIFPAQYLYSTPEQLCKKLQGLCRRPDIARRHVVKVMARSPNTPVYSSEGVLFIVCRRGGCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUJ6</accession>
    <name>A0A673AUJ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein lin-7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">lin7b</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Postsynaptic density membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the lin-7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AUJ6"/>
    <dbReference type="Ensembl" id="ENSSORT00005034232.1">
        <property type="protein sequence ID" value="ENSSORP00005033325.1"/>
        <property type="gene ID" value="ENSSORG00005015718.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUJ6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097025">
        <property type="term" value="C:MPP7-DLG1-LIN7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007269">
        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06796">
        <property type="entry name" value="PDZ_Lin-7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000039">
        <property type="entry name" value="Lin-7 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017365">
        <property type="entry name" value="LIN7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051109">
        <property type="entry name" value="MAM_complex_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14063">
        <property type="entry name" value="PROTEIN LIN-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038039">
        <property type="entry name" value="Lin-7_homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="8 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="L27" evidence="16">
        <location>
            <begin position="23"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="15">
        <location>
            <begin position="106"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="218" mass="24195" checksum="14EBF290791F1990" modified="2020-06-17" version="1">MMSSYYHPGKEADMAAMTEPLCLERDVSRVIELLDRLQRSGELPPPKLQALQRVLQSKFCAAIREVYEQLYDTLDIVGGPEVRAQATAKATVAAFAASEGHAHPRVVELPKTEEGLGFNIMGGKEQNSPIYISRVIPGGVADRQGGLKRGDQLLSVNGVSVEGEHHEKAVELLKAAQGSVKLVVRYTPKVLEEMEARFEKMRSARRRQQHTMQLESLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWW5</accession>
    <name>A0A673AWW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Splicing factor 45</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RNA-binding motif protein 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rbm17</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associates with the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds SXL. Associates with the spliceosome. Interacts with SF3B1, SF1 and U2AF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030004885.1">
        <property type="nucleotide sequence ID" value="XM_030149025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AWW5"/>
    <dbReference type="Ensembl" id="ENSSORT00005035127.1">
        <property type="protein sequence ID" value="ENSSORP00005034211.1"/>
        <property type="gene ID" value="ENSSORG00005016157.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429509"/>
    <dbReference type="CTD" id="84991"/>
    <dbReference type="InParanoid" id="A0A673AWW5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12647">
        <property type="entry name" value="RRM_UHM_SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000079">
        <property type="entry name" value="Putative splicing factor 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040052">
        <property type="entry name" value="RBM17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034653">
        <property type="entry name" value="SPF45_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288:SF8">
        <property type="entry name" value="SPLICING FACTOR 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288">
        <property type="entry name" value="SPLICING FACTOR 45 SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031066">
        <property type="entry name" value="Splicing_factor_SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="G-patch" evidence="12">
        <location>
            <begin position="237"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="323"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="114"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="278"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR031066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="419" mass="45919" checksum="A91AB4530F8CB4B7" modified="2020-06-17" version="1">MSLYDDLGVGASDTKTEGWSKNFKLLQSQLKVKKAALTQAKTQRMKQTTVLAPVIDLKRGGSSDDRQIADTAPHAAAGLKDTVPSGFSSGDVLIPLADEYDPMFPNDYEKVVKRHREERQRQREQERQKEIEEREKKRKDRHDGGAPSGFSRFPAAEGESDDEEEYEKERRKRSMGGAAIAPPSSLVDRDGSASFSYEDEGRPARGSKAAIPPPIYDDSDRPRSPPGPTSSFLANMGGTVAHKIMQKYGFKEGQGLGKHEQGLSTALSVEKTSKRGGKIIVGDAAEKPGSSQSAETSGGGSAADPSKKSETNPLTEILKNPTKVVLLRNMVGRGEVDEDLEGETKEECEKYGKVVKCVIFEIADVPDDEAVRIFLEFERVESAIKAVVDLNGRYFGGRVVKACFYNLDKFRVLDLGEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1H8</accession>
    <name>A0A673B1H8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 24">Calreticulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">calr</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="26">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Cytoplasmic vesicle</location>
            <location evidence="23">Secretory vesicle</location>
            <location evidence="23">Cortical granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 24">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
            <location evidence="3">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 24 28">Belongs to the calreticulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B1H8"/>
    <dbReference type="FunCoup" id="A0A673B1H8">
        <property type="interactions" value="853"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036170.1">
        <property type="protein sequence ID" value="ENSSORP00005035234.1"/>
        <property type="gene ID" value="ENSSORG00005016631.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B1H8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060473">
        <property type="term" value="C:cortical granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000002">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000113">
        <property type="entry name" value="Calreticulin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000122">
        <property type="entry name" value="Calreticulin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009169">
        <property type="entry name" value="Calreticulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF16">
        <property type="entry name" value="CALRETICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002356">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00805">
        <property type="entry name" value="CALRETICULIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0007">Acetylation</keyword>
    <keyword evidence="17 26" id="KW-0106">Calcium</keyword>
    <keyword evidence="21 24 28" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="22" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="20 27" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 24 28" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0430">Lectin</keyword>
    <keyword evidence="11 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="12 28" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025710871" description="Calreticulin" evidence="28">
        <location>
            <begin position="18"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="207"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="339"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="212"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="29">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="29">
        <location>
            <begin position="386"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="27">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00037865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRNR" id="PIRNR002356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="50197" checksum="87D38B77696B3CD1" modified="2020-06-17" version="1">RLVVMTALSLLLVAVSAASVLSESTVFYREQFEDGDAWQSRWVESKHKSDYGKFVLTAGKFYGDAEKDKGLQTSQDARFYAMSSRFSDFSNQGQPLVIQFTVKHEQSIDCGGGYIKLFPSSLNQEDMHGDSTYNIMFGPDICGPGTKKVHVIFNYKGKNHLINKDIRCKDDEYTHLYTLIVNPDNTYEVKIDNKKVEFGSLEEDWDFLPPKKIKDPEAKKPEDWDDREKIPDPDDKKPEDWDKPENIPDPDAKKPDDWDEEMDGEWEPPMVTNPDYKGEWKPREIDNPAYKGKWIHPETDNPEYTADSEIYKYDSIGVIGLDLWQVKSGTIFDNFLITNDPNLAEEVGNDTWDKTRDAEKKMKESQEEEERKKREEEDKKRREEAKEEEEEEEDEEKDEDEDEEEEEEHEEEEEEEEEDEGTDSKLKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6B8</accession>
    <name>A0A673B6B8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">orc2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ORC2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B6B8"/>
    <dbReference type="Ensembl" id="ENSSORT00005036956.1">
        <property type="protein sequence ID" value="ENSSORP00005035998.1"/>
        <property type="gene ID" value="ENSSORG00005016944.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B6B8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056773">
        <property type="entry name" value="WHD_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24882">
        <property type="entry name" value="WHD_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="8">
        <location>
            <begin position="176"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 2 winged-helix" evidence="9">
        <location>
            <begin position="394"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="466" mass="53204" checksum="370034012EA3BE96" modified="2020-06-17" version="1">ARTPGKTVSFSKTPNTEPSTPTRTGRSRNNNQINLYHKKVQFVSTTPHRLRKRMTAPSLRSDSDSELSPSDSEAEEEDEESTEQEHKVKKEDKENVKAPRTPSKNLSAALYKTPAKKSKSSEPVHQPSMVEEYFEAHGSSKVLTSDRTLERLHTPKLDRETLIRLLEGKPSCYSKEIQQLHKKYCKHFNKWMLQLKLGFSVLVYGLGSKKALLEEFRVSHLSQEIHLVVNGFFPSITLKSILNALTCEVFDHQGSFRTPADQIQFISKTLKDSPDLNVYLIIHNIDGPMLRGEKTQCALGQLASLPNLHLVASIDHINAPLVWDQFKQSQFNWLWWECVTFQHYAEETSYENSLLVQQTGALALSSLTHVLRSLTPNARGIFKLLVKFQLENKDNPSYTGLSFQDFYQRCREAFLVNSDLTLRTQLTEFRDHKLIRTRKGADGVEYLIVPVEANTLVDFLENEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B7N3</accession>
    <name>A0A673B7N3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Allograft inflammatory factor 1-like EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">aif1l</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Ruffle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030005060.1">
        <property type="nucleotide sequence ID" value="XM_030149200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B7N3"/>
    <dbReference type="FunCoup" id="A0A673B7N3">
        <property type="interactions" value="613"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039328.1">
        <property type="protein sequence ID" value="ENSSORP00005038336.1"/>
        <property type="gene ID" value="ENSSORG00005017928.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429605"/>
    <dbReference type="CTD" id="83543"/>
    <dbReference type="InParanoid" id="A0A673B7N3"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097178">
        <property type="term" value="P:ruffle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000106">
        <property type="entry name" value="Allograft inflammatory factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049025">
        <property type="entry name" value="AIF-1_EF_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042433">
        <property type="entry name" value="AIF1/AIF1L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10356:SF5">
        <property type="entry name" value="ALLOGRAFT INFLAMMATORY FACTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10356">
        <property type="entry name" value="ALLOGRAFT INFLAMMATORY FACTOR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21008">
        <property type="entry name" value="AIF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Allograft inflammatory factor 1-like EF-hand" evidence="14">
        <location>
            <begin position="8"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="148" mass="16784" checksum="EB64796DC548C35B" modified="2020-06-17" version="1">MPSSHNVQGGKAFGQLKEQQRQKLEEINKEYLEDQKYRDEEDLAEKLEGFKNKYAEFDLNDQGEIDLMGLKRTMEKLGVPKTHLELKKMIVEVTGGSSNTINYRDFVKMMLGKRSAVLKLVLIFEDKANGSPCKPSGPPPKRDIASLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAM8</accession>
    <name>A0A673BAM8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Oocyte zinc finger protein XlCOF6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115416577</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029986249.1">
        <property type="nucleotide sequence ID" value="XM_030130389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BAM8"/>
    <dbReference type="Ensembl" id="ENSSORT00005039879.1">
        <property type="protein sequence ID" value="ENSSORP00005038880.1"/>
        <property type="gene ID" value="ENSSORG00005018181.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416577"/>
    <dbReference type="InParanoid" id="A0A673BAM8"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
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</entry>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BH79</accession>
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        </recommendedName>
    </protein>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BH79"/>
    <dbReference type="FunCoup" id="A0A673BH79">
        <property type="interactions" value="1306"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040715.1">
        <property type="protein sequence ID" value="ENSSORP00005039703.1"/>
        <property type="gene ID" value="ENSSORG00005018526.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BH79"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="4">
        <location>
            <begin position="26"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="81"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="109"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="172" mass="17484" checksum="B03C618ACEB362B2" modified="2020-06-17" version="1">MAEVAPAPAAAPAKAAKKKASKPKMAGPSVGDLILKAVSASKERSGVSLATLKKALAAGGYDVDKNKARVKTAVKKLVLKGALVQTKGTGASGQDEQEDRDQGQEKAAKSPKKAAKSPKKAAKSPKKAAKKAPAAKKAPAKKAAKAKAATKKAPAKKAAPKKKSPSLRQDPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJI6</accession>
    <name>A0A673BJI6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Esterase OVCA2</fullName>
            <ecNumber evidence="5">3.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">OVCA2 serine hydrolase domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ovca2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Exhibits ester hydrolase activity with a strong preference for long-chain alkyl ester substrates and high selectivity against a variety of short, branched, and substituted esters. Is able to hydrolyze ester bonds within a wide range of p-nitrophenyl derivatives (C2-C14) in vitro, with a strong preference toward substrates of &gt;8 carbons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a carboxylic ester + H2O = an alcohol + a carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:33308"/>
            <dbReference type="EC" id="3.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LovG family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_030010965.1">
        <property type="nucleotide sequence ID" value="XM_030155105.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030010966.1">
        <property type="nucleotide sequence ID" value="XM_030155106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BJI6"/>
    <dbReference type="FunCoup" id="A0A673BJI6">
        <property type="interactions" value="1231"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042992.1">
        <property type="protein sequence ID" value="ENSSORP00005041920.1"/>
        <property type="gene ID" value="ENSSORG00005019454.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433616"/>
    <dbReference type="CTD" id="124641"/>
    <dbReference type="InParanoid" id="A0A673BJI6"/>
    <dbReference type="OrthoDB" id="414698at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032526">
        <property type="term" value="P:response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000073">
        <property type="entry name" value="esterase OVCA2 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="10">
        <location>
            <begin position="3"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="221" mass="24733" checksum="F8499E7F39671BF1" modified="2020-06-17" version="1">MAPLRVLCIHGYRQNASSFREKTGALRKLLKKNVELLYVSAPHSVQQVNNEEVPEKEKSSGGDDDPRGWWFSDVQARSFSAQQQCEESLGLEESVTVVREAVKAQGPFDGILGFSQGAAFVAMLCSLQEQKLEPELNFRFAILVAGFPSACTEHNRFYSAPIQIPSLHVFGQEDRVIPDNMSLELLPSFQDPQTLRHPGGHFVPAASAHKQTYLDFLKRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJK7</accession>
    <name>A0A673BJK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">45 kDa calcium-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Stromal cell-derived factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sdf4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CREC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029994072.1">
        <property type="nucleotide sequence ID" value="XM_030138212.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029994073.1">
        <property type="nucleotide sequence ID" value="XM_030138213.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029994074.1">
        <property type="nucleotide sequence ID" value="XM_030138214.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029994076.1">
        <property type="nucleotide sequence ID" value="XM_030138216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BJK7"/>
    <dbReference type="FunCoup" id="A0A673BJK7">
        <property type="interactions" value="620"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041840.1">
        <property type="protein sequence ID" value="ENSSORP00005040787.1"/>
        <property type="gene ID" value="ENSSORG00005019038.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422129"/>
    <dbReference type="CTD" id="51150"/>
    <dbReference type="InParanoid" id="A0A673BJK7"/>
    <dbReference type="OrthoDB" id="9978834at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16225">
        <property type="entry name" value="EFh_CREC_cab45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000120">
        <property type="entry name" value="45 kDa calcium-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000207">
        <property type="entry name" value="Putative 45 kDa calcium-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027240">
        <property type="entry name" value="CAB45_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF98">
        <property type="entry name" value="45 KDA CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025440289" description="45 kDa calcium-binding protein" evidence="12">
        <location>
            <begin position="31"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="93"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="132"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="233"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="273"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="357" mass="42242" checksum="3C039CDCC3635B43" modified="2020-06-17" version="1">MAIWRKMWARNLLSMSLFLYVLLHTIDVHARPANMSASKDKNPTNKDENEILPPDHLNGVKMELDGHLNKDFHQEVFLGKEMEEFEEDSEPRRNRKKLIEIFTKVDFNKDRSVSAKEMQRWIMEKTEEHFQEAMRENKNSFRAVDPDGDGHVTWDEYRVKFLASKGFNEKEIAEKIKNNEDLKLDEETQEVLESLKDRWFQADNPPADQLLNEQEFLSFLHPEHSRGMLKYMVKEIVRDLDQDGDKKLTLSEFISLPVGTVENQQAQDIDDDWVRERRKEFEDVIDSNHDGIVTMEELEEYMDPMNEYNALNEAKQMIAVADENQNHSLELDEILKYSEYFTGSKLMDYARNVHEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJZ9</accession>
    <name>A0A673BJZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Choline/ethanolamine transporter FLVCR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Heme transporter FLVCR1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">flvcr2a</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Uniporter that mediates the transport of extracellular choline and ethanolamine into cells, thereby playing a key role in phospholipid biosynthesis. Choline and ethanolamine are the precursors of phosphatidylcholine and phosphatidylethanolamine, respectively, the two most abundant phospholipids. Transport is not coupled with proton transport and is exclusively driven by the choline (or ethanolamine) gradient across the plasma membrane. Also acts as a heme b transporter that mediates heme efflux from the cytoplasm to the extracellular compartment.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>choline(out) = choline(in)</text>
            <dbReference type="Rhea" id="RHEA:32751"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ethanolamine(in) = ethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:32747"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>heme b(in) = heme b(out)</text>
            <dbReference type="Rhea" id="RHEA:75443"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the major facilitator superfamily. Feline leukemia virus subgroup C receptor (TC 2.A.1.28.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BJZ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005041691.1">
        <property type="protein sequence ID" value="ENSSORP00005040638.1"/>
        <property type="gene ID" value="ENSSORG00005018969.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJZ9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097037">
        <property type="term" value="P:heme export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000184">
        <property type="entry name" value="Feline leukemia virus subgroup C receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF3">
        <property type="entry name" value="HEME TRANSPORTER FLVCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0265">Erythrocyte maturation</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="366"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="494"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="23">
        <location>
            <begin position="70"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="519" mass="58412" checksum="8F3D8CDCBCF8F8CF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKU4</accession>
    <name>A0A673BKU4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Opioid receptor kappa 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115411394</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BKU4"/>
    <dbReference type="Ensembl" id="ENSSORT00005044350.1">
        <property type="protein sequence ID" value="ENSSORP00005043256.1"/>
        <property type="gene ID" value="ENSSORG00005019997.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BKU4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038048">
        <property type="term" value="F:dynorphin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019233">
        <property type="term" value="P:sensory perception of pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001418">
        <property type="entry name" value="Opioid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF1">
        <property type="entry name" value="KAPPA-TYPE OPIOID RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00384">
        <property type="entry name" value="OPIOIDR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="58"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="312" mass="34971" checksum="ABCF043DABE29405" modified="2020-06-17" version="1">TSVRKAAAAPPPGAQCHPQTLTKPQCDHDDSLEPESMSPIIPIITAVYSVVFVVGLLGNCLVMYVIIRYTKMKTATNIYIFNLALADALVTTTMPFQSTDYLLNTWPFGEVVCKVFISIDYYNMFTSIFTLTMMSVDRYVAVCHPVKALDFRTPIKTNSDITECALQFPEPYVYWDTLMKVCVFSVRMLSGSREKDRNLRRITRLVVVVVAVFVICWTPIHIFILVKALVSVPETTAIMAAYFFCVALGYTNSSLNPVLYAFLDENFKRCFKDFCLSAKLKGDKVSGSRRGLSTVREVAVPLENPDRTGKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNK5</accession>
    <name>A0A673BNK5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycerophosphodiester phosphodiesterase domain containing 5b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gdpd5b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BNK5"/>
    <dbReference type="Ensembl" id="ENSSORT00005045423.1">
        <property type="protein sequence ID" value="ENSSORP00005044301.1"/>
        <property type="gene ID" value="ENSSORG00005020421.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BNK5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344:SF6">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="229"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="581" mass="66854" checksum="679325017F41BCD8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRK7</accession>
    <name>A0A673BRK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyridoxal kinase</fullName>
            <ecNumber evidence="9">2.7.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Pyridoxine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">pdxkb</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively. PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>pyridoxal + ATP = pyridoxal 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17310"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:10225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>pyridoxamine + ATP = pyridoxamine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57761"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:25105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>pyridoxine + ATP = pyridoxine 5'-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25108"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16709"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:25109"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxal: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxine 5'-phosphate from pyridoxine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673BRK7"/>
    <dbReference type="FunCoup" id="A0A673BRK7">
        <property type="interactions" value="1189"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043938.1">
        <property type="protein sequence ID" value="ENSSORP00005042848.1"/>
        <property type="gene ID" value="ENSSORG00005019832.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRK7"/>
    <dbReference type="OrthoDB" id="2104723at2759"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00298"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000007">
        <property type="entry name" value="Pyridoxal kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0007">Acetylation</keyword>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="26">
        <location>
            <begin position="80"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="307" mass="34235" checksum="BEBCC2B330832329" modified="2020-06-17" version="1">MECRVLSIQSHVVRGYVGNKSASFPLQVLGFEVDSINSVQFSNHTGYSHWKGQVLTADELHVLYEGIKLNNVHHYDYVLTGYTRDTSFLEMVVDIVQELKRANPNLVYVCDPVLGDHGSMYVPQNLYPVYKNKVVPVADIITPNQFEAELLTGKNISTEKDAVEVMDLLHAMGPDTVVITSSDLPSRLGDRFLVSLGSQRHVRPDGSRTTQRVRIEVPKVDAVFVGTGDLFAAMLLAWTHHYPNDLKTACEKTFSVMHHVIQRTISYAHELAGPGRRPSPPQLELRMVQSKADIEDPAIVTEATVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BS10</accession>
    <name>A0A673BS10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prospero domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115415542</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BS10"/>
    <dbReference type="Ensembl" id="ENSSORT00005045082.1">
        <property type="protein sequence ID" value="ENSSORP00005043964.1"/>
        <property type="gene ID" value="ENSSORG00005020284.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BS10"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070365">
        <property type="term" value="P:hepatocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070309">
        <property type="term" value="P:lens fiber cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001945">
        <property type="term" value="P:lymph vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.500:FF:000001">
        <property type="entry name" value="Prospero homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.500">
        <property type="entry name" value="Homeo-prospero domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023082">
        <property type="entry name" value="Homeo_prospero_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037131">
        <property type="entry name" value="Homeo_prospero_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039350">
        <property type="entry name" value="Prospero_homeodomain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12198">
        <property type="entry name" value="HOMEOBOX PROTEIN PROSPERO/PROX-1/CEH-26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12198:SF9">
        <property type="entry name" value="PROSPERO HOMEOBOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05044">
        <property type="entry name" value="HPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51818">
        <property type="entry name" value="HOMEO_PROSPERO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Prospero" evidence="8">
        <location>
            <begin position="368"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="522" mass="58850" checksum="D5E0247720967111" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BS42</accession>
    <name>A0A673BS42_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">trypsin</fullName>
            <ecNumber evidence="8">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673BS42"/>
    <dbReference type="Ensembl" id="ENSSORT00005046766.1">
        <property type="protein sequence ID" value="ENSSORP00005045611.1"/>
        <property type="gene ID" value="ENSSORG00005020956.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BS42"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000010">
        <property type="entry name" value="Kallikrein related peptidase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF20">
        <property type="entry name" value="TRYPSIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="24"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="252" mass="27890" checksum="C53FA375D23FEFB9" modified="2020-06-17" version="1">MQPIFLYFPLCVVGQDFAAADVRVIGGYAPVPHSLKYIVTLQTRLRQHLSDFFCSFHLLLKNNMVIVAGDYSLSIYEGTEQEVFPQVLIPHPEYDSNTYNNDIMLIKLQVPVYLNSFVSITLMPRPNAIISEGRTCRVSGWGNTEPSGGQIPSTLRTVKVPIISLEKCNSTDSFNGTITENMLCAGFSTGGKDACQGDSGGPLVCDGRLYGVVSWGKGCAEPNFPGVYTAVAKYRKWIDNTIFSFYRRCDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUD1</accession>
    <name>A0A673BUD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">CCR4-NOT transcription complex subunit 4</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">CCR4-associated factor 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">E3 ubiquitin-protein ligase CNOT4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Potential transcriptional repressor NOT4Hp</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase CNOT4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">cnot4a</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15. Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA. As part of the PINK1-regulated signaling, upon mitochondria damage, ubiquitinates ABCE1 and thereby recruits autophagy receptors to the mitochondrial outer membrane to initiate mitophagy.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 34">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with CNOT1 via its C-terminus but does not stably associate with the CCR4-NOT complex. Interacts (via RING domain) with UBE2D2. Interacts with ABCE1, PINK1 and PELO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673BUD1"/>
    <dbReference type="FunCoup" id="A0A673BUD1">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046555.1">
        <property type="protein sequence ID" value="ENSSORP00005045405.1"/>
        <property type="gene ID" value="ENSSORG00005020819.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUD1"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000006">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000044">
        <property type="entry name" value="Putative ccr4-not transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16 27" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 25" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 26" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 26" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="29">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="30">
        <location>
            <begin position="109"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="31">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="26">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="256"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="318"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="404"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="493"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="549"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="645"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="756"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="28">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="645"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00077837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00083942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="798" mass="86362" checksum="5E74A0DEC3AAE495" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUZ9</accession>
    <name>A0A673BUZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">pre-mRNA 3' end processing protein WDR33</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">WD repeat-containing protein 33</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">WD repeat-containing protein of 146 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115437182</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the cleavage and polyadenylation specificity factor (CPSF) module of the pre-mRNA 3'-end processing complex. Interacts with CPSF3/CPSF73.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the WD repeat WDR33 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BUZ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005046099.1">
        <property type="protein sequence ID" value="ENSSORP00005044964.1"/>
        <property type="gene ID" value="ENSSORG00005020664.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUZ9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000069">
        <property type="entry name" value="WD repeat domain 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000077">
        <property type="entry name" value="WD repeat domain 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000085">
        <property type="entry name" value="WD repeat domain 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5 18" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="157"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="198"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="240"/>
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    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="284"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="327"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="371"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="568"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="602"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="631"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="680"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="693"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="711"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="740"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="790"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="800" mass="88552" checksum="78CABB07578EC4AB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BV24</accession>
    <name>A0A673BV24_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Homeobox protein OTX1 B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">otx1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BV24"/>
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        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047891.1">
        <property type="protein sequence ID" value="ENSSORP00005046726.1"/>
        <property type="gene ID" value="ENSSORG00005021376.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BV24"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003406">
        <property type="term" value="P:retinal pigment epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000142">
        <property type="entry name" value="homeobox protein OTX2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003026">
        <property type="entry name" value="Otx1_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003025">
        <property type="entry name" value="Otx_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013851">
        <property type="entry name" value="Otx_TF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793:SF9">
        <property type="entry name" value="HOMEOBOX PROTEIN OTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03529">
        <property type="entry name" value="TF_Otx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01256">
        <property type="entry name" value="OTX1HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01255">
        <property type="entry name" value="OTXHOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="36"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="38"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="332" mass="35734" checksum="B6402F230F616F2A" modified="2020-06-17" version="1">MMSYLKQPPYGVNGLGLSGAAMDLLHPSVGYPATPRKQRRERTTFTRSQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQSGSSAKARPPKKKSSPARESTGSESSGQFTPPAVSSTGSSSSSSSSTNHTGPAALSSTSTSISTVSSIWSPAISPGNAPAPAVAPLPEPGPPSNASCMQRSMSGSAAAATSYPMSYNQTPGYGQGYPTPSSSYFSGVDCGSYLAPMHSHHHPHQLSPMTASSMSGHPHHHISQSSGHHHHHHHHQAYGGSGLAFNSSDCLDYKEQTAASSWKLNFNTTDCLDYKDQASWRFQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVG0</accession>
    <name>A0A673BVG0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">cyld3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673BVG0"/>
    <dbReference type="Ensembl" id="ENSSORT00005045273.1">
        <property type="protein sequence ID" value="ENSSORP00005044153.1"/>
        <property type="gene ID" value="ENSSORG00005020348.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVG0"/>
    <dbReference type="OrthoDB" id="6287070at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000004">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase CYLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000007">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase CYLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000206">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CAP-Gly" evidence="16">
        <location>
            <begin position="141"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="16">
        <location>
            <begin position="305"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="16">
        <location>
            <begin position="494"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="15">
        <location>
            <begin position="594"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="189"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="398"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="978" mass="108670" checksum="60A746B8653D41C4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYG3</accession>
    <name>A0A673BYG3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">THAP domain-containing protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115430994</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030007077.1">
        <property type="nucleotide sequence ID" value="XM_030151217.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030007078.1">
        <property type="nucleotide sequence ID" value="XM_030151218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BYG3"/>
    <dbReference type="Ensembl" id="ENSSORT00005047236.1">
        <property type="protein sequence ID" value="ENSSORP00005046079.1"/>
        <property type="gene ID" value="ENSSORG00005021106.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430994"/>
    <dbReference type="InParanoid" id="A0A673BYG3"/>
    <dbReference type="OrthoDB" id="5982876at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052224">
        <property type="entry name" value="THAP_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927">
        <property type="entry name" value="AGAP005574-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="276"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="424" mass="47736" checksum="BE482C185D3927CB" modified="2020-06-17" version="1">MPKYCCVPNCRTESGSGSGTGTRKSFYKFPLHDPDRLQLWLRNMGRQNWTPSRHQYICHKHFGPSSFKVRWGIRYLENDAVPTLFQEGEKRKASEHGERKAKRFRADGNQSVTASHDRTAAALHLYEVTVAPSEQNPTGLLEPSEAAEWDDCVQASGVGSADGRWMEAELPLTLFQTVEDFSDGDEYEVVLMSEGQEDQLVDGITAAILTQVHGSTAEDVTADELAAAHPDERPRVIAYFETIPNVLPCEAQVVFSPETVVSSSLSSEPITSTLPIVSKHVPPSPTSLKMKVKRVDAEEEEEEEEEEEEEEEDSGFECPGRQLEEHCYHRNSVSRRQLEAIVSELQKKVKVLQQRHRRHLEKLLGLENTVNQLRQSNLLNQERLQLLQGADAQTSPAVPDVAQTVDVYSEEEDDDTYLCTSEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYG5</accession>
    <name>A0A673BYG5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">F-box protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fbxo10</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BYG5"/>
    <dbReference type="Ensembl" id="ENSSORT00005047941.1">
        <property type="protein sequence ID" value="ENSSORP00005046775.1"/>
        <property type="gene ID" value="ENSSORG00005021401.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BYG5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22090">
        <property type="entry name" value="F-box_FBXO10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000009">
        <property type="entry name" value="F-box only protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000015">
        <property type="entry name" value="F-box only protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000061">
        <property type="entry name" value="F-box protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF15">
        <property type="entry name" value="F-BOX ONLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="953" mass="103948" checksum="8DA0B5656FEB049B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1G8</accession>
    <name>A0A673C1G8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ap1g1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029986723.1">
        <property type="nucleotide sequence ID" value="XM_030130863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C1G8"/>
    <dbReference type="Ensembl" id="ENSSORT00005050263.1">
        <property type="protein sequence ID" value="ENSSORP00005049066.1"/>
        <property type="gene ID" value="ENSSORG00005022244.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416968"/>
    <dbReference type="CTD" id="164"/>
    <dbReference type="InParanoid" id="A0A673C1G8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000030">
        <property type="entry name" value="AP-1 complex subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017107">
        <property type="entry name" value="AP1_complex_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037094">
        <property type="entry name" value="AP1_complex_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GAE" evidence="11">
        <location>
            <begin position="700"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="820" mass="91151" checksum="9E2795351BCC0231" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C451</accession>
    <name>A0A673C451_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Actin, alpha skeletal muscle A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">acta1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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            <authorList>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="9">Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.</text>
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            <location evidence="1">Cytoplasm</location>
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        <text evidence="2 10">Belongs to the actin family.</text>
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            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00023097"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00038582"/>
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        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="377" mass="41945" checksum="1C7185C0F7C19A26" modified="2020-06-17" version="1">MCDDDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDAGDGVTHNVPVYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETAYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5F7</accession>
    <name>A0A673C5F7_9TELE</name>
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        <submittedName>
            <fullName evidence="12">Protocadherin 1 gamma 31</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <location>
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            <end position="717"/>
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        </location>
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        </location>
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            <end position="677"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="880" mass="96744" checksum="C4A7CE3B8AD83D1C" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5P3</accession>
    <name>A0A673C5P3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pre-B-cell leukemia transcription factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Homeobox protein PBX1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115437116</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/PBX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016098.1">
        <property type="nucleotide sequence ID" value="XM_030160238.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051961.1">
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        <property type="gene ID" value="ENSSORG00005022987.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437116"/>
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    <dbReference type="Proteomes" id="UP000472271">
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    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000277">
        <property type="entry name" value="Pre-B-cell leukemia transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005542">
        <property type="entry name" value="PBX_PBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03792">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51978">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PBC" evidence="17">
        <location>
            <begin position="36"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="229"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="14">
        <location>
            <begin position="231"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="392"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="428" mass="46623" checksum="0396E7C8D522745A" modified="2020-06-17" version="1">MDEQPRLMHSHGVGMAGHPGLAQHMQDGTAGTDGEGRKQDIGDILQQIMTITDQSLDEAQARKHALNCHRMKPALFNVLCEIKEKTVLSIRGAQEEEPPDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAATGGVGADNSAEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCSITVSQVSNWFGNKRIRYKKNIGKFQEEANMYAARTAVNATSVSAHGSQANSPSTPNSAGSAGSFNMSNSGDLFMSVQSLNGDSYQGGQVGANIQSQVDTLRHVISQTGGYSDSLAASQMYSPQGINTNGGWQDAPTPSSVTSPTEGPGSVHSDTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7X8</accession>
    <name>A0A673C7X8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dazap1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C7X8"/>
    <dbReference type="Ensembl" id="ENSSORT00005050555.1">
        <property type="protein sequence ID" value="ENSSORP00005049354.1"/>
        <property type="gene ID" value="ENSSORG00005022413.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C7X8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF18">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="14"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="99"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="253"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="290"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="378"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="410" mass="43990" checksum="544699535A12B555" modified="2020-06-17" version="1">QFSNIAGAVFVFARNYFSQYGEVVDCVIMKDKTTNQSRGFGFVKFKDPNCVRTVLETKPHNLDGRNIDPKPCTPRGMQPEKSRTKEGWKGSKADSNKSKKIFVGGIPHNCGEPELRDYFNRFGVVSIAECFGFITFEAEQSVDQAVNMHFHDIMGKKVEVKKAEPRDSKAPGQLGPGQWAPRGILSAANGWTAQPAQGWQQPYGPQGVWVSTTGQPIGGYGPPLTAGRGTPTQPPSPFNAFLVTTPAGSFAAPQGYPQQGYSTAPQFGYSFGTPQADQYAPQVPPPPTTPGTAPLGFAPATTPTQDLSKAPTGQPDFPYSQYGLGNYPQDPSAYGPTRPSHSYGQDEQGGYSAGYSQDLTAFGHSFADPSQPTASYGAPTAQPAAAPQPAANAFGRGQNHNVQGFHPYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CA80</accession>
    <name>A0A673CA80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protocadherin-8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">si:ch211-199f5.1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Calcium-dependent cell-adhesion protein. May play a role in activity-induced synaptic reorganization underlying long term memory. Could be involved in CDH2 internalization through TAOK2/p38 MAPK pathway. In hippocampal neurons, may play a role in the down-regulation of dendritic spines, maybe through its action on CDH2 endocytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">The N-terminal extracellular domain forms homophilic interactions; these interactions activate p38 MAPK via TAOK2 and trigger endocytosis. Interacts with CDH2; this interaction may lead to CDH2 cointernalization. Interacts with CDH11. Interacts with TAOK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CA80"/>
    <dbReference type="FunCoup" id="A0A673CA80">
        <property type="interactions" value="231"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050270.1">
        <property type="protein sequence ID" value="ENSSORP00005049073.1"/>
        <property type="gene ID" value="ENSSORG00005022309.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CA80"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000120">
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        <property type="entry name" value="protocadherin-8 isoform X1"/>
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        <property type="entry name" value="Cadherin_N"/>
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    <sequence length="984" mass="108421" checksum="A204B15A1CFDE102" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="8929" checksum="77F065554246B8A9" modified="2020-06-17" version="1">TSGFCGGAACPEWKTSGRSDERSPDCLRIVLIGKTGSGKSSSGVCVVCPTAAVMVRSQRPCFCSSTIRPHSKQTKLWILFMTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CIV7</accession>
    <name>A0A673CIV7_9TELE</name>
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        <recommendedName>
            <fullName evidence="11">Plastin-2</fullName>
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    </protein>
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        <name evidence="9" type="primary">lcp1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CIV7"/>
    <dbReference type="FunCoup" id="A0A673CIV7">
        <property type="interactions" value="1611"/>
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        <property type="protein sequence ID" value="ENSSORP00005055526.1"/>
        <property type="gene ID" value="ENSSORG00005024698.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:actin filament bundle assembly"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21333">
        <property type="entry name" value="CH_PLS2_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21292">
        <property type="entry name" value="CH_PLS_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000263">
        <property type="entry name" value="plastin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000012">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000014">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000025">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF35">
        <property type="entry name" value="PLASTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="12"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="114"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="258"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="396"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="517"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="70642" checksum="440896DCAA94B6B9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKD4</accession>
    <name>A0A673CKD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor SOX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sox11a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CKD4"/>
    <dbReference type="FunCoup" id="A0A673CKD4">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055946.1">
        <property type="protein sequence ID" value="ENSSORP00005054670.1"/>
        <property type="gene ID" value="ENSSORG00005024468.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKD4"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048593">
        <property type="term" value="P:camera-type eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000007">
        <property type="entry name" value="Transcription factor SOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017386">
        <property type="entry name" value="SOX-12/11/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF113">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038098">
        <property type="entry name" value="SOX-12/11/4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="13">
        <location>
            <begin position="46"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="11">
        <location>
            <begin position="46"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="257"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="259"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="377" mass="41698" checksum="F170AC45E8FD9DB0" modified="2020-06-17" version="1">MVQHTDNSETDGSMSREATDSEESEFMACSPVAINPDWCKTATGHIKRPMNAFMVWSKIERRKIMEQSPDMHNAEISKRLGKRWKMLKDSEKIPFIREAERLRLKHMADYPDYKYRPKKKPKLDSSKSSAPSPEKCAKMAKTPSKKCTKIKTKSVSKNTHGVFPEDCVFPSLKVPKTVKSELTDEDDDDEYEEDYRMGIKSGGEEERMRPYNVAKVPASPTLSSSAESEGTSMYEEVRNNRLFYNIKNITKQSTLHAASVSPASSRSVSTSSSSSSSSSSSSSSSSSSSSSSSSGEDADDLLFDFSLNFASSGPGSELGNPNSGNLSLSLVDKDLDSFSEGSLGSHFEFPDYCTPELSEMIAGDWLEANFSDLVFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLM2</accession>
    <name>A0A673CLM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IRG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CLM2"/>
    <dbReference type="FunCoup" id="A0A673CLM2">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054177.1">
        <property type="protein sequence ID" value="ENSSORP00005052923.1"/>
        <property type="gene ID" value="ENSSORG00005023844.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLM2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04104">
        <property type="entry name" value="p47_IIGP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF10">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="38"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="377" mass="42098" checksum="676431A9AD952A65" modified="2020-06-17" version="1">SRIHRSYCIFPSVEALKTGDAALIAEKVKEYLEKHEQVPLNIAVTGETGSGKSSFVNAFRGVDNKDENAAATGCVETTKEVKSYNHPKYPNVTLWDLPGIGTTMFPADKYLKLVGFKKFDFFIIISADRFRENDAKLAQEIQKMGKKFYFVRTKIDLDMKNQERSQRDFNEKNNLHRIRKNCIEGLEKLLIPSPKVFLISNFDLHLYDFERLCNTLEKELPQHQQHVLLLALPNISQEVCNKKREVLQAKIQYYALMSAVGAAVPLPGTSTVVDASIILAFVTKCEDVFHIDGGELLLSLVKEPVKSGGKLTLALILRVLSSSILSTSLKATEEGTRFIPGIGIPIAMGLSSLTTYNALKFFLDNFTKAALETEGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CM41</accession>
    <name>A0A673CM41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="20">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">usp3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the peptidase C19 family. USP3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="20"/>
    <dbReference type="RefSeq" id="XP_029993044.1">
        <property type="nucleotide sequence ID" value="XM_030137184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CM41"/>
    <dbReference type="FunCoup" id="A0A673CM41">
        <property type="interactions" value="752"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058011.1">
        <property type="protein sequence ID" value="ENSSORP00005056711.1"/>
        <property type="gene ID" value="ENSSORG00005025209.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421339"/>
    <dbReference type="CTD" id="9960"/>
    <dbReference type="InParanoid" id="A0A673CM41"/>
    <dbReference type="OrthoDB" id="21192at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000234">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000019">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF19">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 20" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 20" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9 20" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="23">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="22">
        <location>
            <begin position="160"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="614" mass="68919" checksum="9A62C3662233D1AD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMU9</accession>
    <name>A0A673CMU9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Retinoic acid receptor responder protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Chemerin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CMU9"/>
    <dbReference type="Ensembl" id="ENSSORT00005056818.1">
        <property type="protein sequence ID" value="ENSSORP00005055535.1"/>
        <property type="gene ID" value="ENSSORG00005024787.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CMU9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050994">
        <property type="term" value="P:regulation of lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029562">
        <property type="entry name" value="Chemerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15106">
        <property type="entry name" value="RETINOIC ACID RECEPTOR RESPONDER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15106:SF2">
        <property type="entry name" value="RETINOIC ACID RECEPTOR RESPONDER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025547172" description="Retinoic acid receptor responder protein 2" evidence="10">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="165" mass="18631" checksum="D61077346F9FE301" modified="2020-06-17" version="1">MKMAAMLLWLVCAGVLLFTVEAQDPYDQLPDAYKQGVNLALEQLNSHAGVHHHFRFLRSLDKSEIESGFGVKYLYHHFYLKPTRCAKGTTESNSQTCPFRNDRPLMDCAVCYKTAADQIESNPKPYVHCIQKPRLTEQMRTSRTDHCSKMSYNSGAPTLLAVSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CN01</accession>
    <name>A0A673CN01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">XK-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">xkr5a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the XK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CN01"/>
    <dbReference type="Ensembl" id="ENSSORT00005056895.1">
        <property type="protein sequence ID" value="ENSSORP00005055610.1"/>
        <property type="gene ID" value="ENSSORG00005024814.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CN01"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018629">
        <property type="entry name" value="XK-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050895">
        <property type="entry name" value="XK-related_scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024:SF15">
        <property type="entry name" value="XK-RELATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09815">
        <property type="entry name" value="XK-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="454"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="564"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="575"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU910716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="607" mass="66642" checksum="59312ADABC75C643" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQN7</accession>
    <name>A0A673CQN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="5">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Meiotic recombination protein SPO11-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">spo11</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673CQN7"/>
    <dbReference type="Ensembl" id="ENSSORT00005055572.1">
        <property type="protein sequence ID" value="ENSSORP00005054298.1"/>
        <property type="gene ID" value="ENSSORG00005024338.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CQN7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000387">
        <property type="entry name" value="DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013048">
        <property type="entry name" value="Meiotic_Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01551">
        <property type="entry name" value="SPO11HOMOLOG"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="15">
        <location>
            <begin position="100"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="16">
        <location>
            <begin position="209"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="14">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="389" mass="43557" checksum="5B075D6769C3898B" modified="2020-06-17" version="1">WSFYLVEVNHAILFIKLISFLLYSHSHFNFITSSREILTNIENIILGIVTSLSKDEAPVLTLPNRFSWANVRFDSTIGIQMSSGTSVTTVRSDCPSSVTKFAQILKILSVIYRLVQSNSYATKRDIYYNNTQLFGSQATVDSIVDDISCMLKVPRRSLHVLATSKGLISGDLRYMEEDGTHIDCHSSSAAVAVSSNIGGIRNIVSSARFIMIVEKDATFQRLLDDDFCTKLSPCIIITGKGVPDVNSRLMVRKLWDTLHIPIFALVDADPHGIEIMCIYKYGSLAMSFEAHSLTVPSVMWLGLLPSDLQRLRIPTDVLIPLSKRDQSKLSSLLKRPFLTDQPDWQKEMELMQQNKVKAEIQSLAAIAPDFLTSIYLPNKLRYGGWVGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSA3</accession>
    <name>A0A673CSA3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box protein 16</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CSA3"/>
    <dbReference type="Ensembl" id="ENSSORT00005058513.1">
        <property type="protein sequence ID" value="ENSSORP00005057210.1"/>
        <property type="gene ID" value="ENSSORG00005025376.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052805">
        <property type="entry name" value="GEF_Ubiquitin-Prot_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46857">
        <property type="entry name" value="EPITHELIAL CELL-TRANSFORMING SEQUENCE 2 ONCOGENE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46857:SF2">
        <property type="entry name" value="F-BOX ONLY PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="88"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="251" mass="29160" checksum="320764261C1EE4F7" modified="2020-06-17" version="1">MKIAVSETEGIHSICSMQTKRSAWTPLNHPLSNTKVFDERRNLLAKWFARWSDSQRKAVLQDFVLGCSMDQLCFLSLSVNRRLPLQAADFTCLLPRALSLYLFSFLDPRSLSRCAQVSWHWKSIVELDQLWMPKCLRHGWCIDFSPTPLEQGVWKRHYIHTVLELRRSPPQQQFVIPDVSTLRSRHEGQSEEACLLSEECCMSACSTTRRLKMEKQSTVTPPWRDSDRCPKDTLRFNYLDNLDPIEQALTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CT88</accession>
    <name>A0A673CT88_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Nuclear cap-binding protein subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">20 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ncbp2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC), promoting the interaction between upf1 and upf2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with srrt/ars2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of parn, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, ncbp2/cbp20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ncbp1/cbp80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13 15">Component of the nuclear cap-binding complex (CBC), a heterodimer composed of ncbp1/cbp80 and ncbp2/cbp20 that interacts with m7GpppG-capped RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RRM NCBP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989081.1">
        <property type="nucleotide sequence ID" value="XM_030133221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CT88"/>
    <dbReference type="SMR" id="A0A673CT88"/>
    <dbReference type="FunCoup" id="A0A673CT88">
        <property type="interactions" value="1624"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056436.1">
        <property type="protein sequence ID" value="ENSSORP00005055158.1"/>
        <property type="gene ID" value="ENSSORG00005024650.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418677"/>
    <dbReference type="CTD" id="22916"/>
    <dbReference type="InParanoid" id="A0A673CT88"/>
    <dbReference type="OrthoDB" id="201398at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12240">
        <property type="entry name" value="RRM_NCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000128">
        <property type="entry name" value="Nuclear cap-binding protein subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027157">
        <property type="entry name" value="NCBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034148">
        <property type="entry name" value="NCBP2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847">
        <property type="entry name" value="20 KD NUCLEAR CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11 15" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="39"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="164" mass="18982" checksum="116BF8C5EE6B7CED" modified="2020-06-17" version="1">MAGKLNALTSDSYIDVSQYRDQHFKGNRYEQEKLLKQSHTLYVGNLSFYTTEEQVHELFSKSGDVKRIIIGLDKIKKTACGFCFVEYYTRADAEHAMRFINGTRLDDRIIRTDWDAGFKEGRQYGRGKSGGQVRDEYRQDYDPARGGYGKLAQQHRVTEGRNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CWH3</accession>
    <name>A0A673CWH3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain containing 148</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ccdc148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CWH3"/>
    <dbReference type="Ensembl" id="ENSSORT00005058370.1">
        <property type="protein sequence ID" value="ENSSORP00005057067.1"/>
        <property type="gene ID" value="ENSSORG00005025334.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CWH3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039902">
        <property type="entry name" value="CCDC148/CCDC112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21549:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21549">
        <property type="entry name" value="MUTATED IN BLADDER CANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="351"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="545" mass="65165" checksum="882870D7A8074BD9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CY52</accession>
    <name>A0A673CY52_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115437788</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016991.1">
        <property type="nucleotide sequence ID" value="XM_030161131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CY52"/>
    <dbReference type="Ensembl" id="ENSSORT00005059086.1">
        <property type="protein sequence ID" value="ENSSORP00005057767.1"/>
        <property type="gene ID" value="ENSSORG00005025572.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437788"/>
    <dbReference type="InParanoid" id="A0A673CY52"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000006">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="453" mass="50987" checksum="E86DA65091587E34" modified="2020-06-17" version="1">MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYQGDSDLQLERINVYYNEASGSKFVPRAILVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEMGEYEEDEIEDEEDVRHDVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAC8</accession>
    <name>A0A672YAC8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytohesin 4a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115423373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YAC8"/>
    <dbReference type="Ensembl" id="ENSSORT00005001219.1">
        <property type="protein sequence ID" value="ENSSORP00005001184.1"/>
        <property type="gene ID" value="ENSSORG00005000709.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAC8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01252">
        <property type="entry name" value="PH_GRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000309">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF315">
        <property type="entry name" value="CYTOHESIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="2"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="197"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="332" mass="38468" checksum="CBE943806998677F" modified="2020-06-17" version="1">MDPKKGIKYLVENNLLEWRAEPVAEFLYKEEGLNKTAIGNLLGEREEMHLEILKAFVALHEFSDLNLVQALRFCFFFLLLSSRQFLWSFRLPGEAQKIDRMMEAFASRYCDCNPGVFQSTDTCYILSFAIIMLNTSLHNPNVKDKPSLQRFVSMNRGINNGDDLPVELLTKLYASIRSEPFKIPEDDGNDLTLTFFNPDREGWLLKIGGRVKTWKRRWFILTDSCLYYFEYTTDKDPIGIIPLENLCVRELQDSSKPYCLELYNPQGQKIKACKMENTGRVVQGKHQSYKLSAASADERDDWIEAIRASVTKDPFYDLVYLRKRKVISNTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCD8</accession>
    <name>A0A672YCD8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aquaporin-7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aqp7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glycerol(in) = glycerol(out)</text>
            <dbReference type="Rhea" id="RHEA:29675"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YCD8"/>
    <dbReference type="Ensembl" id="ENSSORT00005001325.1">
        <property type="protein sequence ID" value="ENSSORP00005001290.1"/>
        <property type="gene ID" value="ENSSORG00005000758.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCD8"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000005">
        <property type="entry name" value="Aquaporin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF15">
        <property type="entry name" value="AQUAPORIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02019">
        <property type="entry name" value="AQUAPORIN7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="305" mass="32332" checksum="ADB46C03C315EC30" modified="2020-06-17" version="1">MKDLVQSIDLGASQRKAVTVTRPRVWIKNEFVRVGLAESLCTYVMMTFGLGSVAQVVTGQGAYGQYVSINLGFGLAVAMGVHVGGKVSGAHMNAAVSFTMCVFGRLAWKMLPLYVFAQFLGSFLAAGTIYAVYYEAIYSYCGGNLTVTGVKATAGIFATYPAPYLSLMGGFIDQVFGTAMLLLCLMALSDQKNKPAAAGNEPVAVGLLVLLIGISLGSNSGYAINPTRDIGPRVFTALAGWGVDVFRSGNGWWWVPLVAPPIGGVIGAGLYKALVELHHPLFSDQDRQALESGEETAPLEKQEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCU1</accession>
    <name>A0A672YCU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YCU1"/>
    <dbReference type="Ensembl" id="ENSSORT00005002903.1">
        <property type="protein sequence ID" value="ENSSORP00005002819.1"/>
        <property type="gene ID" value="ENSSORG00005001755.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCU1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098">
        <property type="entry name" value="AGAP001331-PA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098:SF16">
        <property type="entry name" value="REGULATORY PROTEIN ZESTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="3">
        <location>
            <begin position="15"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="247" mass="27980" checksum="14DD2C491ABF56E0" modified="2020-06-17" version="1">MWRSSGSTPSSSLHKMKFEALELEVLVEEANKHLTELQQRNLNITRRNAIWEEICAKVNAVGKVRRTAEEVKKRAQDLRQRTKEKVAYSTTLANKTGDGPAEENPLSTTEQQVQLWFCDEQIGGLKDMSPGARTCRQRRFGTNTCFSDLNLIKNNLSFFFHGESTSEVRPSTSHSSQTTSAHSRRRPAVEDIDVELQQEQKWQAAALKDGLQLIMQELRSESSVTHVLRRHSGHHCTAGSWSALYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGN9</accession>
    <name>A0A672YGN9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Adhesion G protein-coupled receptor E8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YGN9"/>
    <dbReference type="Ensembl" id="ENSSORT00005003845.1">
        <property type="protein sequence ID" value="ENSSORP00005003736.1"/>
        <property type="gene ID" value="ENSSORG00005002241.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YGN9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
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        <property type="entry name" value="GPCR_2_EMR1-like_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF469">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01128">
        <property type="entry name" value="EMR1HORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 14" id="KW-0245">EGF-like domain</keyword>
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    <keyword evidence="11" id="KW-0675">Receptor</keyword>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025527456" description="Adhesion G protein-coupled receptor E8" evidence="16">
        <location>
            <begin position="25"/>
            <end position="679"/>
        </location>
    </feature>
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        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="473"/>
            <end position="494"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="554"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="624"/>
            <end position="648"/>
        </location>
    </feature>
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        <location>
            <begin position="155"/>
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        <location>
            <begin position="238"/>
            <end position="401"/>
        </location>
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        <location>
            <begin position="408"/>
            <end position="649"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="75064" checksum="370272F7C0426ED4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGZ1</accession>
    <name>A0A672YGZ1_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">C1q domain-containing protein</fullName>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="CEREBELLIN 13-RELATED"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="184" mass="20094" checksum="49AFEA53389648E4" modified="2020-06-17" version="1">RTLGVTMDSDFSLDSALKTENKKNVHFPNILEKTLLKYTAGSTICVSHSLTVKQVAFSASLVGTGQRHFGPFSALYTLAFKHVITNIGNAYNPNTGVFTAPVKGFYIYAPGHPSHGCGAALVKNGRHIFIAYERQPSHASSASNGVTLILEVGDVVFLRLWHHSKIYDDGNHVSTFSDHLLFTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJC0</accession>
    <name>A0A672YJC0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YJC0"/>
    <dbReference type="Ensembl" id="ENSSORT00005004831.1">
        <property type="protein sequence ID" value="ENSSORP00005004695.1"/>
        <property type="gene ID" value="ENSSORG00005002858.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJC0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF114">
        <property type="entry name" value="PCDH2G3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="23"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="131"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="256" mass="28530" checksum="21CEFABDB90EDF37" modified="2020-06-17" version="1">MELNRFALQYYGLAFVVFVLRPISGDVSYSIPEEMKRGSVIGNIAKDLGLDLGGLSARKARIDTEDDNVNYCGVNLNTGDLLVQERIDREGLCEKRASCILKQELVLENPLELHRINIRVQDINDNSPQFKEESVKIEIRESADKGARFLLGEAHDEDIGENAVQGYSLQQNDHFKLNVNAKSGGRKYCELVLDKELDREDKKDIMLLLTAFDGGSPQRSGTVAIHVTVLDANDNEPMATKSNVHPGHNCINTPHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YK32</accession>
    <name>A0A672YK32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YK32"/>
    <dbReference type="Ensembl" id="ENSSORT00005005033.1">
        <property type="protein sequence ID" value="ENSSORP00005004893.1"/>
        <property type="gene ID" value="ENSSORG00005002947.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YK32"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF114">
        <property type="entry name" value="PCDH2G3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="673"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="12"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="120"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="228"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="341"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="438"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="562"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="799" mass="88539" checksum="882494BC99488F21" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLA9</accession>
    <name>A0A672YLA9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Mediator complex subunit 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MED9</name>
        <name evidence="12" type="synonym">med9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 11">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Mediator complex subunit 9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997303.1">
        <property type="nucleotide sequence ID" value="XM_030141443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YLA9"/>
    <dbReference type="FunCoup" id="A0A672YLA9">
        <property type="interactions" value="1299"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005543.1">
        <property type="protein sequence ID" value="ENSSORP00005005385.1"/>
        <property type="gene ID" value="ENSSORG00005003196.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424287"/>
    <dbReference type="InParanoid" id="A0A672YLA9"/>
    <dbReference type="OrthoDB" id="5950777at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21431">
        <property type="entry name" value="Med9-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011425">
        <property type="entry name" value="Med9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039242">
        <property type="entry name" value="MED9_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20844:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20844">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07544">
        <property type="entry name" value="Med9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="99" mass="11236" checksum="B9AAF9B439739DC3" modified="2020-06-17" version="1">MAVAQPKLEKESEDCSLLPLVHDIIKCMDKDSQDVHQDLAKLKAKIQEAREQISTMPGIDSSPQEQQQQLATLREQVRTKNQLLQKYKGLCMFDVPKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMT3</accession>
    <name>A0A672YMT3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Plakophilin 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115431197</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YMT3"/>
    <dbReference type="Ensembl" id="ENSSORT00005006171.1">
        <property type="protein sequence ID" value="ENSSORP00005005921.1"/>
        <property type="gene ID" value="ENSSORG00005003541.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YMT3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000008">
        <property type="entry name" value="plakophilin-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF8">
        <property type="entry name" value="PLAKOPHILIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="362"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="621"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="776"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="776"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="930" mass="102779" checksum="AA99F57A5EBFD85F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQ55</accession>
    <name>A0A672YQ55_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Proline rich and Gla domain 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YQ55"/>
    <dbReference type="Ensembl" id="ENSSORT00005006976.1">
        <property type="protein sequence ID" value="ENSSORP00005006714.1"/>
        <property type="gene ID" value="ENSSORG00005003915.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006988.1">
        <property type="protein sequence ID" value="ENSSORP00005006726.1"/>
        <property type="gene ID" value="ENSSORG00005003922.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF39">
        <property type="entry name" value="TRANSMEMBRANE GAMMA-CARBOXYGLUTAMIC ACID PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="4">
        <location>
            <begin position="23"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="210" mass="23844" checksum="C9B6E572653AAF2A" modified="2020-06-17" version="1">HERVKYTFLTGKDAHSVLKRFPRANGFLEELRQGNIERECVEESCSFEEANEVFENKERTMEFWKTRSVYTVNSNSEGRSERADTVYMVVPLLGVALLIIIGLFLLWRCQLQKATRRRPAYTQNRYLANNRSTRSLPRGGGGDSHGLHQQNTRALYVQDLSLSVASRLSGATPPPSYEEVTGHLESSGDETSAPSYSDPPPKYEEIIKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQ83</accession>
    <name>A0A672YQ83_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115437930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030017194.1">
        <property type="nucleotide sequence ID" value="XM_030161334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YQ83"/>
    <dbReference type="FunCoup" id="A0A672YQ83">
        <property type="interactions" value="1608"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007018.1">
        <property type="protein sequence ID" value="ENSSORP00005006756.1"/>
        <property type="gene ID" value="ENSSORG00005003938.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437930"/>
    <dbReference type="InParanoid" id="A0A672YQ83"/>
    <dbReference type="OrthoDB" id="5861309at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027844">
        <property type="entry name" value="INTS15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14540">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14540:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14964">
        <property type="entry name" value="INTS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="466" mass="51641" checksum="34C4F2E71B0866D9" modified="2020-06-17" version="1">MGDIRQSLLPRDVLNAAKELLYHLDIYICNMVQSGRQPPQVDSKTLDLVEEFILHAPKDRNAPVRRMSAIQELQLLEIMCSCFQEQSRDTVRQLVFSALFSLQGNQADESRMALLGKLVSMAVAVGRVPILECAATWLQRTHRVYCVRLAQVLVDDYCSMVPGSVPTLQNIHSASPRFCCQFITAVTTLYDLSSEELTPPLELLQMIVSWIQDDPRLVLITFLNTPLSGSQPVSSLDVTPLGGLVRWCVKAPLAYRKDKKQALTNSSSENEAEVGHLFSALHLSVLQVFMLLPNILNEKGLFGRLALLQMESLAALTSDLSRLLDQADKHTHTSSADTHALSQLALDRLAQALQVAMANRALLCSREDLRAICSRLPHNNLLQLVLSGPVMYYNNIHTPPMAFSPHASHSPIASHTTHPAHPTHPTLPTHPTHAPHAPLASHPSPHPQYPAQPFMTGMPFPFRPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQQ4</accession>
    <name>A0A672YQQ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM/homeobox protein Lhx1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Homeobox protein Lim-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC115433189</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030010326.1">
        <property type="nucleotide sequence ID" value="XM_030154466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YQQ4"/>
    <dbReference type="Ensembl" id="ENSSORT00005007187.1">
        <property type="protein sequence ID" value="ENSSORP00005006924.1"/>
        <property type="gene ID" value="ENSSORG00005004008.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433189"/>
    <dbReference type="InParanoid" id="A0A672YQQ4"/>
    <dbReference type="OrthoDB" id="10068367at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09367">
        <property type="entry name" value="LIM1_Lhx1_Lhx5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09375">
        <property type="entry name" value="LIM2_Lhx1_Lhx5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000006">
        <property type="entry name" value="LIM homeobox transcription factor 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000075">
        <property type="entry name" value="LIM/homeobox protein Lhx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000046">
        <property type="entry name" value="LIM/homeobox protein Lhx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049618">
        <property type="entry name" value="Lhx1/5_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049619">
        <property type="entry name" value="Lhx1/5_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF106">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN LHX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="62"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="175"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="177"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="137"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="331"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="370"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="399" mass="44676" checksum="E11839D3B2698D04" modified="2020-06-17" version="1">MLHCESCEKPILDRFLLKVLDRPWHIKCVRCCECKCSLTEKCFSREGRLYCKNDFFRRFGTKCGGCAQGILPSDLVRRAKSKVFHLNCFTCMMCNKQLSTGEELYILDEFKFVCKEDYQNNNGKDTILLSVTTCSDPSLSPDSQDPQDDGKDSETGHLSDKEVCNNENDEQSAVGKRRGPRTTIKAKQLETLKAAFAATPKPTRHIREQLSRETGLSMRVIQVWFQNRRSKERRMKQLSSLGARRHVFFRGPRRMRALGERLEPGELGHFSYYGDYPGEYYGSGGNYEYFQGPPPSQAQTPADLGFVPSSVPAGTPLGALDHHHPGHHCPTEVQCFSDTMSHHPADSPSPEPNGPGSMHSISSEMCGPSTPFTTVALSDNGYTNQLSQPSSELSEGTVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTI5</accession>
    <name>A0A672YTI5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Meis homeobox 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115415052</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/MEIS homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YTI5"/>
    <dbReference type="Ensembl" id="ENSSORT00005008171.1">
        <property type="protein sequence ID" value="ENSSORP00005007888.1"/>
        <property type="gene ID" value="ENSSORG00005004439.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTI5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000004">
        <property type="entry name" value="Meis2 homeobox isoform 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032453">
        <property type="entry name" value="PKNOX/Meis_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="185"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="187"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="311" mass="34967" checksum="9AEE8A6B51C266E9" modified="2020-06-17" version="1">MWLNTHTHTHTHTHTHICKCYVLMGHVAECIHPYVCVYIYIYIYIYIYIYIYIYIYIYIYICLCIIVVCVCVCVCVQVHELCDNFCHRYISCLKGKMPIDLVIEDRDVCKADFDDLSGSSTNLADHNPASWRDLDDAHSTPSVGTPGPSSGGHVSQSGDNTSDLGDGLDNSLASPGTGDEDDQDKKRQKKRGIFPKVATNIMRAWLFQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVSQGTAYSPDGQPMGGFVLDGQQHMGLRPGGKRSRTHLEGPSCTDLTLFLCKCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YX40</accession>
    <name>A0A672YX40_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115431831</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YX40"/>
    <dbReference type="Ensembl" id="ENSSORT00005009608.1">
        <property type="protein sequence ID" value="ENSSORP00005009301.1"/>
        <property type="gene ID" value="ENSSORG00005005093.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YX40"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000013">
        <property type="entry name" value="Calcium-sensing receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF0">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025534742" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="12">
        <location>
            <begin position="24"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="599"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="669"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="757"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="793"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="825"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="599"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="867" mass="95933" checksum="17691ABD908E152E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYH5</accession>
    <name>A0A672YYH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ectonucleotide pyrophosphatase/phosphodiesterase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YYH5"/>
    <dbReference type="Ensembl" id="ENSSORT00005009982.1">
        <property type="protein sequence ID" value="ENSSORP00005009658.1"/>
        <property type="gene ID" value="ENSSORG00005005277.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYH5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
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        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF107">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="27309" checksum="74DEFA7E2B0A169D" modified="2020-06-17" version="1">LKSVPSPCADGCTSCFLQLIEALQGVDRIMAQLMNGLKQLRLHRCVNVIIVADHGMESTSCDRMEVLEDLVGDIRSLWVTEGPFGRIRAKDKHTCKKPDQKIKAYLKSNLPKRLHFANSRRIEDVNVLVDAKWLYPGSLTFCSGGAHGYDNDFESMHAMFLSYGPQFQPNTQVEPFSNVELYNLMCDPSVQNNGTHGSLNHILRRPFFSPKGPEEGSVPGQCPLVSLDPADGLGCSCPTLVSRLCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZI4</accession>
    <name>A0A672YZI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA replication complex GINS protein PSF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gins3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.</text>
    </comment>
    <comment type="function">
        <text evidence="6">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029992113.1">
        <property type="nucleotide sequence ID" value="XM_030136253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YZI4"/>
    <dbReference type="Ensembl" id="ENSSORT00005010524.1">
        <property type="protein sequence ID" value="ENSSORP00005010191.1"/>
        <property type="gene ID" value="ENSSORG00005005535.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420752"/>
    <dbReference type="CTD" id="64785"/>
    <dbReference type="InParanoid" id="A0A672YZI4"/>
    <dbReference type="OrthoDB" id="10251744at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11713">
        <property type="entry name" value="GINS_A_psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
        <property type="entry name" value="PSF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF3 N-terminal" evidence="8">
        <location>
            <begin position="21"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="93"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="211" mass="24027" checksum="3FBE52C9F1CDCF94" modified="2020-06-17" version="1">MDTQSYLPVQPGVGMEENFLSLDDILLSHERLPTRTECAFPRLGFLEKSSDTQDIPEGTKMELPLWLSKGLYEKKRRVLSVELPKVYREGWRTVFTADPNVVDLHKMGPYYYGLGSQMLHFDSPENPEIAQALLQTFIGRFRRIMDSSQNAYNEDTSALVERLDGLEKALFRSGQSGLNGFQSWEKGEASTLTASSLVLNYRKRKITDVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZL0</accession>
    <name>A0A672YZL0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sarcoglycan, alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sgca</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZL0"/>
    <dbReference type="FunCoup" id="A0A672YZL0">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010140.1">
        <property type="protein sequence ID" value="ENSSORP00005009812.1"/>
        <property type="gene ID" value="ENSSORG00005005332.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZL0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008908">
        <property type="entry name" value="Sarcoglycan_alpha/epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048347">
        <property type="entry name" value="Sarcoglycan_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048346">
        <property type="entry name" value="Sarcoglycan_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10132">
        <property type="entry name" value="ALPHA-/EPSILON-SARCOGLYCAN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10132:SF16">
        <property type="entry name" value="ALPHA-SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05510">
        <property type="entry name" value="Sarcoglycan_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20989">
        <property type="entry name" value="Sarcoglycan_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025538582" evidence="4">
        <location>
            <begin position="22"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Sarcoglycan alpha/epsilon N-terminal" evidence="5">
        <location>
            <begin position="28"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Sarcoglycan alpha/epsilon second" evidence="6">
        <location>
            <begin position="130"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="418" mass="47312" checksum="1709EA4CA2215D0C" modified="2020-06-17" version="1">YRFCFFFFFPVCTVTFLGANAEIKFTIPVGKLFTYELMRETFQNDFEPLSKLYAGRLNDDPMIFKCNLQNFPDLPEWLRFTQRHPFDNGFLYGTPTSPGKSIIEIYAINKRSYETARHILVIKIVPEKILPYQAEFFIELREIEKVLPSSVQDEIQSDLQKLWGTKALEIVNITNALDRGGRVPLPLAGHFEGVYVKVGSEQYFSECLLRVLTPEHTKQCSAKDKGGKDKIWSGCLFCNIPSNCITWCKTELFDLSKEKPAPPAPTMGPGILEDVMEFNPPESPPSRDYFPDYIVTVIVPLILAIILCLILGYVMFGRREGMYNKTLQTPVLIQLHHHHTIHDNTDELRGMAANRGVPPPLSTLPMFNSRTGERAMPLQSDSPSIPLIMAQQSQVSNMWRGGQIQPAKGSSPALGMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1I7</accession>
    <name>A0A672Z1I7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutathione transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z1I7"/>
    <dbReference type="Ensembl" id="ENSSORT00005011057.1">
        <property type="protein sequence ID" value="ENSSORP00005010715.1"/>
        <property type="gene ID" value="ENSSORG00005005727.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1I7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03183">
        <property type="entry name" value="GST_C_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000008">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040077">
        <property type="entry name" value="GST_C_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF9">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE THETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="102"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="235" mass="27200" checksum="695C21D1889B217F" modified="2020-06-17" version="1">MYFNCIFLEKENISLKKWKFHGNSQLLFHVILHLTCKITVCFCHFIDIFLYLKNTGKICQINGQQYSDEFGKVSVMRKVPVMKDGIPAAETSSLVPDHWYPDLQLRARVNEYLSWQHMTIRAQGSKVFLLRAMFPIIMGSEAPKDKMDAAVEELKQSVDLLEQKFLQDKAFLVSDRICVADVVAVVELMQVQEDARPKLVAWRQRVTAELGPKLFDEAHEVPLLQKNQDCDRFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2I4</accession>
    <name>A0A672Z2I4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Sphingomyelin phosphodiesterase 3</fullName>
            <ecNumber evidence="6">3.1.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Neutral sphingomyelinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Neutral sphingomyelinase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="primary">LOC115420759</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-O-octadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-octadecyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74001"/>
            <dbReference type="ChEBI" id="CHEBI:75216"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:39924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycerol + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:75542"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:41120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>N-(15Z-tetracosenoyl)sphing-4-enine-1-phosphocholine + H2O = N-(15Z-tetracosenoyl)-sphing-4-enine + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45320"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74450"/>
            <dbReference type="ChEBI" id="CHEBI:74535"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:45321"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-(hexadecanoyl)-sphing-4-enine-1-phosphocholine + H2O = N-hexadecanoylsphing-4-enine + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72959"/>
            <dbReference type="ChEBI" id="CHEBI:78646"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:45645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>N-(tetracosanoyl)-sphing-4-enine-1-phosphocholine + H2O = N-tetracosanoyl-sphing-4-enine + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72965"/>
            <dbReference type="ChEBI" id="CHEBI:83360"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:45325"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a sphingomyelin + H2O = phosphocholine + an N-acylsphing-4-enine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17636"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="3.1.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:19254"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a sphingosylphosphocholine + H2O = a sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:84410"/>
            <dbReference type="ChEBI" id="CHEBI:85171"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:45297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>an N-(acyl)-sphingosylphosphocholine + H2O = an N-acyl-sphingoid base + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45300"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64583"/>
            <dbReference type="ChEBI" id="CHEBI:83273"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:45301"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Golgi apparatus membrane</location>
            <topology evidence="20">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z2I4"/>
    <dbReference type="FunCoup" id="A0A672Z2I4">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011342.1">
        <property type="protein sequence ID" value="ENSSORP00005010968.1"/>
        <property type="gene ID" value="ENSSORG00005005891.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2I4"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006684">
        <property type="term" value="P:sphingomyelin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09078">
        <property type="entry name" value="nSMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000015">
        <property type="entry name" value="sphingomyelin phosphodiesterase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017766">
        <property type="entry name" value="Sphingomyelinase/PLipase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF8">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="33" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="34">
        <location>
            <begin position="272"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="173"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00050994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00051412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00068544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00076605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00081703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="602" mass="65901" checksum="A5E38A15BC0873C6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2S9</accession>
    <name>A0A672Z2S9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Calmegin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">clgn</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the calreticulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z2S9"/>
    <dbReference type="FunCoup" id="A0A672Z2S9">
        <property type="interactions" value="811"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011878.1">
        <property type="protein sequence ID" value="ENSSORP00005011496.1"/>
        <property type="gene ID" value="ENSSORG00005002840.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2S9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000001">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000430">
        <property type="entry name" value="Si:ch211-274f20.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF7">
        <property type="entry name" value="CALMEGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00805">
        <property type="entry name" value="CALRETICULIN_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 15" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025718168" evidence="15">
        <location>
            <begin position="17"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="245"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="496"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="249"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601580-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="538" mass="61451" checksum="52F1E3AAF55154D6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4Z1</accession>
    <name>A0A672Z4Z1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aldehyde dehydrogenase 7 family, member A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aldh7a1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z4Z1"/>
    <dbReference type="Ensembl" id="ENSSORT00005012314.1">
        <property type="protein sequence ID" value="ENSSORP00005011925.1"/>
        <property type="gene ID" value="ENSSORG00005006319.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z4Z1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07130">
        <property type="entry name" value="ALDH_F7_AASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000015">
        <property type="entry name" value="alpha-aminoadipic semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF1">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="60"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="280"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="452" mass="48727" checksum="4B9F33C85940089F" modified="2020-06-17" version="1">NLTEQAPQCVHGAIQSEWSTHSAAMSGLLINQPKYAWLKELGLSEDNPGMYNGKWGGSGEVVTSYCPANNEPIARVTQATMAEYEETVQKTKEAWKIWADIPAPKRGEIVRQIGDALRKKIQVLGSLVSLEMGKIYVEGVGEVQEYVDVCDYAVGLSRMIGGPILPSERPGHALIEQWNPVGLVGIITAFNFPVAVYGWNNAIALTCGNVCLWKGAPTTPLTSVAVTKIVAEVLEQNNLPGAICSMTCGGADIGTAMAKDERVDLLSFTELGGNNAIIVTLYGPLHTKQAVEQYLAAVEQAKQQGGTVICGGKVMDRPGNYVEPTIITGLAHDAPIVHTETFVPILYVLKFKTEEEAFAWNNEVQQGLSSSIFTKDMGRVFRWLGPKGSDCGIVNVNIPTSGAEIGGAFGGEKHTGGGRESGSDSWKQYMRRSTCTINYSKDLPLAQGIKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z687</accession>
    <name>A0A672Z687_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="22">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC115439086</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="22"/>
    <dbReference type="Ensembl" id="ENSSORT00005012389.1">
        <property type="protein sequence ID" value="ENSSORP00005011997.1"/>
        <property type="gene ID" value="ENSSORG00005004350.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z687"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005899">
        <property type="term" value="C:insulin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005009">
        <property type="term" value="F:insulin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043560">
        <property type="term" value="F:insulin receptor substrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048009">
        <property type="term" value="P:insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05032">
        <property type="entry name" value="PTKc_InsR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000050">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000087">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000108">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000026">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000001">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000002">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
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        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
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    <keyword evidence="7" id="KW-0732">Signal</keyword>
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            <end position="774"/>
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            <end position="773"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1307"/>
            <end position="1321"/>
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        <location>
            <begin position="1322"/>
            <end position="1335"/>
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            <position position="1134"/>
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        <location>
            <position position="1010"/>
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    </evidence>
    <sequence length="1388" mass="156348" checksum="432998EE6D775560" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6A0</accession>
    <name>A0A672Z6A0_9TELE</name>
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            <fullName evidence="14">Cytochrome P450 1A</fullName>
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        </recommendedName>
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        <name evidence="15" type="primary">cyp1a</name>
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    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="14">Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.</text>
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        <reaction evidence="17">
            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
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        </reaction>
    </comment>
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        <cofactor evidence="1 12 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Microsome membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cytochrome P450 family.</text>
    </comment>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:iron ion binding"/>
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    </dbReference>
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        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:hormone biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:progesterone metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CYP1A"/>
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        <property type="entry name" value="CYTOCHROME P450 1A"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01683">
        <property type="entry name" value="EP450ICYP1A"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0492">Microsome</keyword>
    <keyword evidence="9 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="518" mass="58669" checksum="94949117FE5F64A1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6K7</accession>
    <name>A0A672Z6K7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115429195</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004319.1">
        <property type="nucleotide sequence ID" value="XM_030148459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z6K7"/>
    <dbReference type="FunCoup" id="A0A672Z6K7">
        <property type="interactions" value="1104"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012861.1">
        <property type="protein sequence ID" value="ENSSORP00005012464.1"/>
        <property type="gene ID" value="ENSSORG00005006548.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429195"/>
    <dbReference type="InParanoid" id="A0A672Z6K7"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000024">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="180" mass="20330" checksum="504BF272B9472798" modified="2020-06-17" version="1">MGLTISSLFSKFFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVAESAEELSKMIQEDELKEAVLLVFANKQDLPNAMGVSELTDKLGLHSLRSRTWHVQATCATQGNGLYEGLDWLSNELAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8X7</accession>
    <name>A0A672Z8X7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">fMet-Leu-Phe receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115428395</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030003285.1">
        <property type="nucleotide sequence ID" value="XM_030147425.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030003286.1">
        <property type="nucleotide sequence ID" value="XM_030147426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z8X7"/>
    <dbReference type="Ensembl" id="ENSSORT00005013708.1">
        <property type="protein sequence ID" value="ENSSORP00005013300.1"/>
        <property type="gene ID" value="ENSSORG00005006888.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428395"/>
    <dbReference type="InParanoid" id="A0A672Z8X7"/>
    <dbReference type="OrthoDB" id="10044919at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14974">
        <property type="entry name" value="7tmA_Anaphylatoxin_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000034">
        <property type="entry name" value="G-protein coupled receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002234">
        <property type="entry name" value="Anphylx_rcpt_C3a/C5a1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF48">
        <property type="entry name" value="C3A ANAPHYLATOXIN CHEMOTACTIC RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
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</entry>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="552" mass="58222" checksum="FDFDBE98CD3F6E05" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCU0</accession>
    <name>A0A672ZCU0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="10">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115422413</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:15842"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZCU0"/>
    <dbReference type="Ensembl" id="ENSSORT00005014699.1">
        <property type="protein sequence ID" value="ENSSORP00005014277.1"/>
        <property type="gene ID" value="ENSSORG00005007274.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZCU0"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000013">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 10" id="KW-0521">NADP</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="49"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="228"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="531" mass="60775" checksum="697CF4BBEBDF66E2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHE4</accession>
    <name>A0A672ZHE4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fam185a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029992640.1">
        <property type="nucleotide sequence ID" value="XM_030136780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZHE4"/>
    <dbReference type="FunCoup" id="A0A672ZHE4">
        <property type="interactions" value="286"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017196.1">
        <property type="protein sequence ID" value="ENSSORP00005016686.1"/>
        <property type="gene ID" value="ENSSORG00005008441.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421072"/>
    <dbReference type="CTD" id="222234"/>
    <dbReference type="InParanoid" id="A0A672ZHE4"/>
    <dbReference type="OrthoDB" id="5984441at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025164">
        <property type="entry name" value="Toastrack_DUF4097"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34094">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34094:SF1">
        <property type="entry name" value="PROTEIN FAM185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13349">
        <property type="entry name" value="DUF4097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4097" evidence="1">
        <location>
            <begin position="201"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="379" mass="40626" checksum="29CBB22FBE524844" modified="2020-06-17" version="1">MFWSAAGPLRSFSPRLFSTLRCPLHTCRCFSTSTGVRSVTEPESKPPLRQWALAVSPFSSVRAHLGCSISVRPLDLHAFPEADRAFVTVHGTGSDQDLELCVDQCHVLYDEQSRELRISADRVHSGVAIDLRAPIKSNLFITTQGEGNVHVQKMECDICKVQTEKGSCTLHSVKGHQVQVQTKGGQVTGLGTIHGNVDIITSGDSVVDVKKLQGTKMNVSTEHGSLKVKAIYAESSCVSSCSGRVELGHVHGNATVKNVSGDTVIDGSNRFLKVSSHSGDIDIYVGDSGTAEVLSEEGAVCVRVPSSLRAGVELCGASVDVSPEVVLHGAENNTDEGQTTVTGFINGEPHHDQWVKARANRGSVRLKTQSWFESLKLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJR3</accession>
    <name>A0A672ZJR3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rho GTPase activating protein 45b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">arhgap45b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSSORT00005017903.1">
        <property type="protein sequence ID" value="ENSSORP00005017385.1"/>
        <property type="gene ID" value="ENSSORG00005006542.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJR3"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20816">
        <property type="entry name" value="C1_GMIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000016">
        <property type="entry name" value="Rho GTPase activating protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039341">
        <property type="entry name" value="CFAP99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="494"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="558" mass="65320" checksum="19F1E180F619D07C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZP10</accession>
    <name>A0A672ZP10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-conjugating enzyme E2 G1</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E2 ubiquitin-conjugating enzyme G1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein G1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-protein ligase G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ube2g1b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZP10"/>
    <dbReference type="Ensembl" id="ENSSORT00005018679.1">
        <property type="protein sequence ID" value="ENSSORP00005018138.1"/>
        <property type="gene ID" value="ENSSORG00005008994.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZP10"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23795">
        <property type="entry name" value="UBCc_UBE2G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000018">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="14">
        <location>
            <begin position="4"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="169" mass="19268" checksum="10759B59524B2F85" modified="2020-06-17" version="1">MTEQSSLLLRKQLAELNKNPVEGFSAGLIDDEDIYQWEVVVIGPQDTLFEGGFFKATLTFPRDYPLRPPKMKFITEIWHPNVAKNGDVCISILHEPGEDKYGYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDPTGVFKKKVARCVRRSQEMAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZRY8</accession>
    <name>A0A672ZRY8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZRY8"/>
    <dbReference type="Ensembl" id="ENSSORT00005019703.1">
        <property type="protein sequence ID" value="ENSSORP00005019147.1"/>
        <property type="gene ID" value="ENSSORG00005009424.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZRY8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF3">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ion transport N-terminal" evidence="8">
        <location>
            <begin position="76"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="136"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26251" checksum="B0A0811FEA83BC01" modified="2020-06-17" version="1">MEDKSNSFSSNKEEKADGNNVFQRQDSIQKNNTGSQNMKEHSNSVGFKGEREEAMVGFDDLEGASRQYGFMQRQFGAMMQPGVNKFSLRMFGSQKAVEKGAGAGPDGRVWIIHPYSDLGKGRKETLFYWDLIITTTTWLVFNVASDTIFLVDLVMNFRTGIVNEESSEIILDPKVIKMNYLKSWFVVDFLSSIPVDYIFLIVEKGFDSEVYKTARALRIVRFTKILSLLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZSB0</accession>
    <name>A0A672ZSB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flj13639</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dhrs12la</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZSB0"/>
    <dbReference type="Ensembl" id="ENSSORT00005020752.1">
        <property type="protein sequence ID" value="ENSSORP00005020185.1"/>
        <property type="gene ID" value="ENSSORG00005009823.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZSB0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052992">
        <property type="entry name" value="SDR_member_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44656">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44656:SF2">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="326" mass="36121" checksum="4C5685B6477643FB" modified="2025-10-08" version="2">MGGSTMSLYRNSAWFLKGLTEFTRTAFLSASKRFVEKDLEVSVAGRSFMITGANSGIGKATAMAIAKRGGTIHMVCRNKDKAEEARADIVKESGNKEIYVHILDLSETKKVWEFAEAFKRKYKALNVLINNAGSIMSQRDVNAEGLEKGFATNVLGVYILTKSLIPLLEKSADPRVITVSSGGMLVQKLRTGNLQSDRGGRYDGTMVYAQHKRQQVVMTEQLAKTHTNIHFSVMHPGWVDTPAVANAMPDFHRSMKESLRTPEQGADTVVWLAISEAATTNPSGHFYQDRKMVSTHLPLAWTHSSALEEQKLMSLLEDLAKTFQPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1B4</accession>
    <name>A0A673A1B4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Myosin light chain 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115429258</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030004435.1">
        <property type="nucleotide sequence ID" value="XM_030148575.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSSORT00005023053.1">
        <property type="protein sequence ID" value="ENSSORP00005022393.1"/>
        <property type="gene ID" value="ENSSORG00005010902.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429258"/>
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    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF30">
        <property type="entry name" value="CARDIAC MYOSIN LIGHT CHAIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="1" id="KW-0518">Myosin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="140"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="207" mass="22875" checksum="C08C62C89475701B" modified="2020-06-17" version="1">MAPKKVEPKKPEPKKPEPKKEATPAAAAAAAKPAPTPEPQPEAPKEPDFDPKSITLEYTPDQIEEFKEAFTLFDRTPTGEMKITYAQCGDVMRALGQNPTNADVLKVLGKPRPEDMSTKMVDFETFLPMLQHISRAKDQGNFEDFVEGLRVFDKEGNGTIMGAELRHVLATLGERMTEDEVDRLMAGQEDANGCINYASFVKHILSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2Q1</accession>
    <name>A0A673A2Q1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polyadenylate-binding protein</fullName>
            <shortName evidence="9">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115422071</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Binds and protects the poly(A) tail of mRNA with or without an AU-rich element (ARE) and prevents mRNA deadenylation. Stimulates the translation of mRNAs to which it is bound during early development.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with dazl in an RNA-independent manner. The C-terminus can self-associate and also interact with the C-terminus of pabpc1, independently of RNA. RRM 1 and RRM 2 interact with both eif4g1 and paip1, and the C-terminus also interacts with paip1. Prior to oocyte maturation, found in a complex with dazl and pum2 proteins and spdy1 mRNA; pum2 dissociates from the complex during maturation. Interacts with the translation termination factor sup35/erf3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A2Q1"/>
    <dbReference type="Ensembl" id="ENSSORT00005024480.1">
        <property type="protein sequence ID" value="ENSSORP00005023780.1"/>
        <property type="gene ID" value="ENSSORG00005011489.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A2Q1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000001">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000021">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000042">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000049">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="99"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="191"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="294"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="11">
        <location>
            <begin position="535"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="619" mass="69425" checksum="D5FF9C54EB4FD066" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3V6</accession>
    <name>A0A673A3V6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CD3 gamma/delta subunit Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A3V6"/>
    <dbReference type="Ensembl" id="ENSSORT00005024012.1">
        <property type="protein sequence ID" value="ENSSORP00005023337.1"/>
        <property type="gene ID" value="ENSSORG00005011294.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A3V6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042105">
        <property type="term" value="C:alpha-beta T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transmembrane signaling receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive thymic T cell selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015484">
        <property type="entry name" value="CD3_esu/gsu/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10570:SF8">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD3 GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10570">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD3 GAMMA CHAIN / DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025462297" description="CD3 gamma/delta subunit Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="34"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19604" checksum="215FDB6DA6E186D0" modified="2020-06-17" version="1">IISICHQQMSTNTINLKVLLPRCLLLLWTLTDAKTSPEIQVTTVSDGIKVVCPTGHTLYHGDVKITTGKIDYRDENTGEYTCKMSDDDTGKAIFVKFRTCDNCVDLNVVFVSRMIVGDIVVKTVIGVAVYLIASQANRTGSVTSNKKSKYPSTFLNDYFHTTKTAQLERSLILKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4L9</accession>
    <name>A0A673A4L9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Poly (ADP-ribose) polymerase family, member 12a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">parp12a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A4L9"/>
    <dbReference type="FunCoup" id="A0A673A4L9">
        <property type="interactions" value="868"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024942.1">
        <property type="protein sequence ID" value="ENSSORP00005024231.1"/>
        <property type="gene ID" value="ENSSORG00005011667.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4L9"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056226">
        <property type="entry name" value="WH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057602">
        <property type="entry name" value="Zfn-CCCH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF6">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24356">
        <property type="entry name" value="WHD_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23466">
        <property type="entry name" value="WWE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25261">
        <property type="entry name" value="zf-CCCH_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="14">
        <location>
            <begin position="387"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="15">
        <location>
            <begin position="508"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="79362" checksum="B7647DA740645B9B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4W7</accession>
    <name>A0A673A4W7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cd151</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with integrins ITGA3:ITGB1, ITGA5:ITGB1, ITGA3:ITGB1 and ITGA6:ITGB4 and with CD9 and CD181. Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3. Interacts with ITGA3; this interaction modulates ITGA3 glycosylation pattern. Interacts with F11R. Interacts with RAC1 and CDC42; these interactions mediate physical association of RAC1 and CDC42 with integrin adhesion receptor complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A4W7"/>
    <dbReference type="Ensembl" id="ENSSORT00005025238.1">
        <property type="protein sequence ID" value="ENSSORP00005024525.1"/>
        <property type="gene ID" value="ENSSORG00005011786.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4W7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03155">
        <property type="entry name" value="CD151_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000005">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF487">
        <property type="entry name" value="CD151 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="252" mass="28191" checksum="93131E1AAB95E459" modified="2020-06-17" version="1">MEARGEKTHTCGTICLKYLLFLFNVLFWLAGGAVLAVGVWTLVEKSDYISLLNSSLYSASAYILIVAGVIVIMTGIIGCCATLKELKSLLIVYLILLLCIFLLEIIAGVMAYINHQELDEELRQNLKVTMQQKYQQPGEESVTQAVDRLQQEFKCCGSNSSSDWSDSIWIQDPQNKRLVPDSCCKTPSDLCGQRDHPSNIYKVEGGCIMKLEEFILNQLYILGAVGIGIAFLQLVGMMFTCCLYRNLKEDPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4X5</accession>
    <name>A0A673A4X5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fdx1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030014594.1">
        <property type="nucleotide sequence ID" value="XM_030158734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A4X5"/>
    <dbReference type="FunCoup" id="A0A673A4X5">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025002.1">
        <property type="protein sequence ID" value="ENSSORP00005024289.1"/>
        <property type="gene ID" value="ENSSORG00005011696.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436032"/>
    <dbReference type="InParanoid" id="A0A673A4X5"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF75">
        <property type="entry name" value="ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="50"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="165" mass="18022" checksum="A4C074076114B5C9" modified="2020-06-17" version="1">MALVTALRRLAHVRICEYTRKTAAVTFGARHTSLKSFTTSTQPLRSDNKVTVHFINRDGEKITVKGSPGDSLLDIVINEDLDFDGFGACEGTLACSTCHLIFDEDVYKNLGPVTDEEMDMLDLAYGLTDTSRLGCQICLTKSLEGMVARVPESVADIRQSKDGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A704</accession>
    <name>A0A673A704_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sosondowah ankyrin repeat domain family member C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115430428</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOWAH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A704"/>
    <dbReference type="Ensembl" id="ENSSORT00005026109.1">
        <property type="protein sequence ID" value="ENSSORP00005025350.1"/>
        <property type="gene ID" value="ENSSORG00005012201.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A704"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058889">
        <property type="entry name" value="WHD_SOWAHA-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491:SF4">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN SOWAHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25877">
        <property type="entry name" value="WHD_SOWAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SOWAHA-C winged helix-turn-helix" evidence="6">
        <location>
            <begin position="21"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="320"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="392" mass="42600" checksum="2C97CE060DD78F8A" modified="2020-06-17" version="1">MASEFRQQVPVRCLKPGGAPVNTADLTEHFRPLRDTFQTVVGSVAFVKTENGIKCVCLTKKGQGDGACSGTGTGDTGVSRHPPRARQVPGSGYGNGGSPRTGPRGSEGSATPKASRRHFLQAMIDSSPQVRRSVAMRGSVYLSSKSDSDSASVASSNPDEDDDPAPVTLDPLEHEWMLCASDGEWASLSRLLAAEPALLLKKDFVTGFTCLHWAAKHGKPKLIALVVNFAKRHGVPISVDVRSNAGYTPLHVAAMHNHTEVVKLLVGAYDANVEIRDYSGKKACQYLSEQAGAQSADCQDGGRWRFSRVLQSNLKPLRLLNNDGGDEADGEGRQQEKPLRRKSSLSGMKPRLQRLRMRTSQIVHSVTFHDTEELEKCGRGSWRSRPKTHFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A7A1</accession>
    <name>A0A673A7A1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">complement subcomponent C1r</fullName>
            <ecNumber evidence="4">3.4.21.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">c1r</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Serine protease component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1R catalyzes the first enzymatic step in the classical complement pathway: it is activated by the C1Q subcomplex of the C1 complex, which associates with IgG or IgM immunoglobulins complexed with antigens to form antigen-antibody complexes on the surface of pathogens. Immunoglobulin-binding promotes the autocatalytic cleavage and activation of C1R. Activated C1R then cleaves and activates C1S, the second protease of the classical complement pathway. It is unclear if C1R activates C1S within single, strained C1 complexes or between neighboring C1 complexes on surfaces.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).</text>
            <dbReference type="EC" id="3.4.21.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Core component of the complement C1 complex, a calcium-dependent complex composed of 1 molecule of the C1Q subcomplex, 2 molecules of C1R and 2 molecules of C1S. The C1Q subcomplex is composed 18 subunits: 3 chains of C1QA, C1QB, and C1QC trimerize to form 6 collagen-like triple helices connected to six globular ligand-recognition modules. Within the C1 complex, C1R is a dimer of identical chains, each of which is activated by cleavage into two chains, heavy and light, connected by disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="25">The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="28">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.41" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673A7A1"/>
    <dbReference type="Ensembl" id="ENSSORT00005025185.1">
        <property type="protein sequence ID" value="ENSSORP00005024472.1"/>
        <property type="gene ID" value="ENSSORG00005011698.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A7A1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000059">
        <property type="entry name" value="Mannan-binding lectin serine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000012">
        <property type="entry name" value="mannan-binding lectin serine protease 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024175">
        <property type="entry name" value="Pept_S1A_C1r/C1S/mannan-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255:SF25">
        <property type="entry name" value="COMPLEMENT C1R SUBCOMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255">
        <property type="entry name" value="COMPLEMENT COMPONENT 1, S SUBCOMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001155">
        <property type="entry name" value="C1r_C1s_MASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="26" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="17 24 28" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19 25" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 25" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="9 28" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="CUB" evidence="29">
        <location>
            <begin position="30"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="29">
        <location>
            <begin position="204"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="31">
        <location>
            <begin position="321"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="31">
        <location>
            <begin position="387"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="30">
        <location>
            <begin position="465"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="23">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="23">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="23">
        <location>
            <position position="654"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="(3R)-3-hydroxyasparagine" evidence="25">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine; by CK2" evidence="25">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24 27">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="323"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24 28">
        <location>
            <begin position="389"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (between heavy and light chains)" evidence="24">
        <location>
            <begin position="452"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="616"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="650"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00093383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR001155-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR001155-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR001155-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR001155-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="704" mass="77815" checksum="8E10EC3CA3524830" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8D1</accession>
    <name>A0A673A8D1_9TELE</name>
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        <submittedName>
            <fullName evidence="10">MARCKS-related protein 1-B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">marcksl1b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MARCKS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A8D1"/>
    <dbReference type="Ensembl" id="ENSSORT00005026214.1">
        <property type="protein sequence ID" value="ENSSORP00005025454.1"/>
        <property type="gene ID" value="ENSSORG00005012250.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A8D1"/>
    <dbReference type="OrthoDB" id="9948538at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:actin filament organization"/>
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        <property type="term" value="P:central nervous system development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002101">
        <property type="entry name" value="MARCKS"/>
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        <property type="entry name" value="MARCKS-RELATED PROTEIN 1-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353">
        <property type="entry name" value="MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MARCKS"/>
    </dbReference>
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        <property type="entry name" value="MARCKS_1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="11"/>
            <end position="23"/>
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        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="173"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="217" mass="21542" checksum="F5B5803C4BB16A6F" modified="2020-06-17" version="1">MGSQASKGEVAAEANAAAADAAAVKTNGQENGHVKTNGDVSTKPDGDAAATNGSAEAAKEPEAGAGGDAIEPAPAADGEAAKPEGEAATKETPKKKKKKFSLKKSFNFKLNLKKSKKSEAVKEEAAAAATAASPEEKPAENGAAAPAEEKKEEVKEEAAAAAAAAPAASAEAPKAEEGAAKEEAPKEEAKEAAAPAPEATKPTEESSSTPAPSEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAC9</accession>
    <name>A0A673AAC9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rapunzel</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AAC9"/>
    <dbReference type="FunCoup" id="A0A673AAC9">
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005025673.1"/>
        <property type="gene ID" value="ENSSORG00005012349.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AAC9"/>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039051">
        <property type="entry name" value="SE-CTX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472:SF7">
        <property type="entry name" value="PROTEIN RAPUNZEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472">
        <property type="entry name" value="RICIN B-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="229" mass="26275" checksum="B2FA9CBDB7123EB7" modified="2020-06-17" version="1">TLDNDEEIVEDQAKLKQGLVKVLQCVATISSAAAVVNPIFGVAGSLIRVVLHHIDDEDIRTLKREFGSVNRALNEISQQNHNTLVQIKKETLDGQYCSVEENLRNQFRKFMEMVEARPEHRQRKKDDFEESYANDLGDQNLHTLYDGVVGKPKLFSRPILEVYLKHSQGDRRTMERLCTRLTYLFCIGLIALMGYAAVIGDDEEGLSEEWAEKMEHVQEKMQEALHRCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABW0</accession>
    <name>A0A673ABW0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Golgi transport 1A</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in fusion of ER-derived transport vesicles with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ABW0"/>
    <dbReference type="Ensembl" id="ENSSORT00005026791.1">
        <property type="protein sequence ID" value="ENSSORP00005026022.1"/>
        <property type="gene ID" value="ENSSORG00005012502.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABW0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF245">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN GOT1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="135" mass="15354" checksum="10F461A82335E2E8" modified="2025-10-08" version="2">MCGSDPCVCLFPEVGIGLMGFGLFFLVFGVLFYFDSVLLAFGNILFLSGLAFIIGFRRTAHFFFQRHKFRGSLFFLGGVALVLCRWPIIGMLVESYGFVLLFRKARCRLLHKVRILFSLVRIYTISPACIYKADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACG0</accession>
    <name>A0A673ACG0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoprotein hormone beta-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Thyrostimulin subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">gphb5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a heterodimeric glycoprotein hormone with GPHA2 able to bind and activate the thyroid-stimulating hormone receptor (TSHR), leading to increased cAMP production. Plays a central role in controlling thyroid cell metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer with GPHA2; this heterodimer interacts with thyroid-stimulating hormone receptor (TSHR), and hence stimulates cAMP production.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycoprotein hormones subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029983100.1">
        <property type="nucleotide sequence ID" value="XM_030127240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ACG0"/>
    <dbReference type="FunCoup" id="A0A673ACG0">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027213.1">
        <property type="protein sequence ID" value="ENSSORP00005026438.1"/>
        <property type="gene ID" value="ENSSORG00005012665.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414047"/>
    <dbReference type="CTD" id="122876"/>
    <dbReference type="InParanoid" id="A0A673ACG0"/>
    <dbReference type="OrthoDB" id="10006958at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002155">
        <property type="term" value="P:regulation of thyroid hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00069">
        <property type="entry name" value="GHB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000029">
        <property type="entry name" value="glycoprotein hormone beta-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001545">
        <property type="entry name" value="Gonadotropin_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11515">
        <property type="entry name" value="GLYCOPROTEIN HORMONE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11515:SF14">
        <property type="entry name" value="GLYCOPROTEIN HORMONE BETA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00068">
        <property type="entry name" value="GHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025359844" description="Glycoprotein hormone beta-5" evidence="12">
        <location>
            <begin position="27"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoprotein hormone subunit beta" evidence="13">
        <location>
            <begin position="42"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="137" mass="15518" checksum="64D9F4151E3B888A" modified="2020-06-17" version="1">MSLKTRTTQWCGWMLLLLLWASPSPDSPPWVEAVNLRRFIGCAVREFTFLARKPGCGGVHITTDACWGRCETWEKPILDPPFIESYQRVCTYNQTRLVTVKLPNCQPHVDPSYTYPVALRCDCGVCQTSTTECITSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AH70</accession>
    <name>A0A673AH70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">stk10</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_030010623.1">
        <property type="nucleotide sequence ID" value="XM_030154763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AH70"/>
    <dbReference type="FunCoup" id="A0A673AH70">
        <property type="interactions" value="1004"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029432.1">
        <property type="protein sequence ID" value="ENSSORP00005028619.1"/>
        <property type="gene ID" value="ENSSORG00005012915.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433386"/>
    <dbReference type="CTD" id="6793"/>
    <dbReference type="InParanoid" id="A0A673AH70"/>
    <dbReference type="OrthoDB" id="10027016at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000081">
        <property type="entry name" value="STE20-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000120">
        <property type="entry name" value="STE20-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022165">
        <property type="entry name" value="PKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051585">
        <property type="entry name" value="STE20_Ser/Thr_Kinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46538:SF2">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46538">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12474">
        <property type="entry name" value="PKK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="36"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="318"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="720"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1020"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="846"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="919"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="509"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="720"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="740"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1020"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1044" mass="120757" checksum="172D29578EA065E8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AI02</accession>
    <name>A0A673AI02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="11">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">uck1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A673AI02"/>
    <dbReference type="FunCoup" id="A0A673AI02">
        <property type="interactions" value="774"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030173.1">
        <property type="protein sequence ID" value="ENSSORP00005029340.1"/>
        <property type="gene ID" value="ENSSORG00005014004.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AI02"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000297">
        <property type="entry name" value="Uridine-cytidine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="13">
        <location>
            <begin position="21"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="275" mass="31412" checksum="71A1E5F918C241EC" modified="2020-06-17" version="1">PAMAAFETLDESERPRHRPFLIGVSGGTASGKSTVCAKIMELLGQNKVDHRQRKVAIISQDSFYRVLTPDQKAKALKGQYNFDHPDAFDNDLMYKTLKDIVEGRVVEVPTYDFVTHSRLEDRITLYPADVVLFEGILVFYPQKVRDMFHMKLFVDTDSDVRLSRRVLRDVNRGRDLEQILTQYTTFVKPAFEEFCLPTKKYADVIIPRGVDNMVAINLIVQHIQDILNGDICKWQRTSINGRGFKRAISEQGNMQTSTANPPGKRILLEPSSRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AI96</accession>
    <name>A0A673AI96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">5-taurinomethyluridine-[tRNA] synthase subunit GTPB3, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">GTP-binding protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">tRNA modification GTPase GTPBP3, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">gtpbp3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">GTPase component of the GTPBP3-MTO1 complex that catalyzes the 5-taurinomethyluridine (taum(5)U) modification at the 34th wobble position (U34) of mitochondrial tRNAs (mt-tRNAs), which plays a role in mt-tRNA decoding and mitochondrial translation. Taum(5)U formation on mammalian mt-tRNA requires the presence of both GTPBP3-mediated GTPase activity and MTO1 catalytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989099.1">
        <property type="nucleotide sequence ID" value="XM_030133239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AI96"/>
    <dbReference type="FunCoup" id="A0A673AI96">
        <property type="interactions" value="955"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029886.1">
        <property type="protein sequence ID" value="ENSSORP00005029066.1"/>
        <property type="gene ID" value="ENSSORG00005013872.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418691"/>
    <dbReference type="CTD" id="84705"/>
    <dbReference type="InParanoid" id="A0A673AI96"/>
    <dbReference type="OrthoDB" id="188276at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070900">
        <property type="term" value="P:mitochondrial tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14858">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000007">
        <property type="entry name" value="tRNA modification GTPase GTPBP3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000924">
        <property type="entry name" value="tRNA modification GTPase GTPBP3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="entry name" value="tRNA modification GTPase MnmE domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00077869"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00081428"/>
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    </evidence>
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        <source>
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            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="512" mass="55548" checksum="C3FBAAFF00CC1172" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJ61</accession>
    <name>A0A673AJ61_9TELE</name>
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        <lineage>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        </source>
    </evidence>
    <sequence length="398" mass="45421" checksum="7AED04D4E4181C50" modified="2020-06-17" version="1">MTSTWGFCSVLCVAIIRLSHGETGHLPPPTELTYTWLDSLTVNVSWKEPVLPDGCEIDHYEVKVQDTEEIKVQGFPEWTGETHFHGDLLTEKSESAQWISSVRAFGRQSCKDLKSTTVTKPIKTKKPKAKVVEDFKCYINKSHLECSWKAVNPSQELSLSFRLCGQHKAPINECDRIYTSGERTVCHIKNKKEKFVQEDICMIATTDTGLSTFMIKYVLPSPKMSISEDGSNLLLKIEESEVAPAHCWRYNVCYTECNTHKPCQESSENVIKVPYDNHCRYEFRYEVRSDRSCVEMRSDWSDVVTYGDSNPDMMMTVVFIIVPVVLSACVILSCYCFRRHSHIICPTIPDPSAIFKEMMMMNGNKDVKPITSMLYSPEEEEQCKITLVAEDSDLHQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJJ0</accession>
    <name>A0A673AJJ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Short-chain dehydrogenase/reductase 3</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115419760</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="11">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>all-trans-retinol + NADP(+) = all-trans-retinal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25033"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.300"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.300" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673AJJ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005029649.1">
        <property type="protein sequence ID" value="ENSSORP00005028833.1"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF691">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="320" mass="36096" checksum="9431C2CC8EE0F821" modified="2020-06-17" version="1">MIFLMDLQMMILDVIYFILRNSVRIVMRPRTKPIDGELVLITGAGGGLGRLFAQEFTKQGAEVVLWDINSSSNEQTAKLVREMGGKASTYTVDVTNKEDVYRNADLVRKDLGRDVTILVNNAGVVAGERMLDCPDEMVERTMKVNCHALFWVRTLNPLVYKTNDISNMTSANFVECLSLVNDYCASKFAAVGFHESLAHELLAEQVEGVKTTLVCPYIVDTGMFEGCKIREEVELFLPPLEPQYCVEQAMNAILIDQPLVCIPRLTYLPFLFRALLPWESNVVAYRFMGSDKCMYPFIDTMKKQASNFNEATSGIPTYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AK97</accession>
    <name>A0A673AK97_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TAFA chemokine like family member 4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAFA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AK97"/>
    <dbReference type="FunCoup" id="A0A673AK97">
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005028762.1"/>
        <property type="gene ID" value="ENSSORG00005013749.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AK97"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020350">
        <property type="entry name" value="Chemokine-like_TAFA"/>
    </dbReference>
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        <property type="entry name" value="TAFA_chemokine-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31770">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31770:SF7">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12020">
        <property type="entry name" value="TAFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="141" mass="15640" checksum="B017869A5FB475A8" modified="2020-06-17" version="1">TIPRSLGKSNTPPSPLSSLFVALLVSYQVKQGTCEVVAVHRCCNKNKIEERSQTVKCSCFPGQVAGTTRARPSCVEASIVAQKWWCQMQPCMEGEECKVLPDLTGWSCSTGNKVKTTKVRDVYLHTCKCQHFLPFLSVADW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AL63</accession>
    <name>A0A673AL63_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AL63"/>
    <dbReference type="Ensembl" id="ENSSORT00005029927.1">
        <property type="protein sequence ID" value="ENSSORP00005029107.1"/>
        <property type="gene ID" value="ENSSORG00005013890.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AL63"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025680543" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="22"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="125" mass="13477" checksum="B4B56B098B184A4A" modified="2020-06-17" version="1">MTLVSVLIWTLLCCCFTGKSRGQVTVTQPGAVSSDLGASTTITCTTSQDVYGSNELNWYQQRDGETPKLLIYYATNRQSGISSRFSGSGSNSHFTLTISDVQAEDAAVYYCQSGHSINSQAVLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMG9</accession>
    <name>A0A673AMG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbonic anhydrase</fullName>
            <ecNumber evidence="4">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115410719</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029978351.1">
        <property type="nucleotide sequence ID" value="XM_030122491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AMG9"/>
    <dbReference type="Ensembl" id="ENSSORT00005030455.1">
        <property type="protein sequence ID" value="ENSSORP00005029622.1"/>
        <property type="gene ID" value="ENSSORG00005014141.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410719"/>
    <dbReference type="InParanoid" id="A0A673AMG9"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF200">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025713580" description="Carbonic anhydrase" evidence="4">
        <location>
            <begin position="22"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="6">
        <location>
            <begin position="24"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="6">
        <location>
            <begin position="283"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="581" mass="65191" checksum="46AC2200912A1E28" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ART0</accession>
    <name>A0A673ART0_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">Nuclear RNA export factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nxf1a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NXF family.</text>
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        <property type="component" value="Chromosome 10"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="UBA_TAP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear RNA export factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000066">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="11">
        <location>
            <begin position="401"/>
            <end position="553"/>
        </location>
    </feature>
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        <location>
            <begin position="582"/>
            <end position="636"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
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            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
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            <end position="78"/>
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    </feature>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="636" mass="71212" checksum="02135474E10AC60F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AT04</accession>
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            <fullName evidence="4">Centrosomal protein 126</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <alternativeName>
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        </lineage>
    </organism>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Glycosyltransferase which regulates the biosynthesis of heparan sulfate (HS). Initiates HS synthesis by transferring the first N-acetyl-alpha-D-glucosamine (alpha-GlcNAc) residue (GlcNAcT-I activity) to the tetrasaccharide linker (GlcA-Gal-Gal-Xyl-)Ser core linker. May also transfer alpha-GlcNAc residues during HS elongation (GlcNAcT-II activity). Lacks glucuronyl transferase II (GlcAT-II) activity. Important for both skeletal development and hematopoiesis, through the formation of HS proteoglycans (HSPGs). Through the synthesis of HS, regulates postnatal pancreatic islet maturation and insulin secretion.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Receptor for REG3A, REG3B and REG3G, induces the activation of downstream signaling pathways such as PI3K-AKT or RAS-RAF-MEK-ERK signaling pathway. Required for the function of REG3A in regulating keratinocyte proliferation and differentiation. Required for the inhibition of skin inflammation mediated by REG3A through the activation of PI3K-AKT-STAT3 pathway. Required for the function of REG3A and REG3G in glucose tolerance in pancreas. Expressed in microglia, is activated by nociceptor-derived REG3G in response to endotoxins, leading to the inhibition of kynurenine pathway to prevent endotoxic death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(alpha-D-GlcNAc-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16221"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="Rhea" id="RHEA-COMP:12574"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="ChEBI" id="CHEBI:132104"/>
            <dbReference type="EC" id="2.4.1.223"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer; disulfide-linked. Interacts with REG3A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.223" evidence="22"/>
    <dbReference type="AlphaFoldDB" id="A0A673AWC9"/>
    <dbReference type="FunCoup" id="A0A673AWC9">
        <property type="interactions" value="1464"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033515.1">
        <property type="protein sequence ID" value="ENSSORP00005032618.1"/>
        <property type="gene ID" value="ENSSORG00005015482.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AWC9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001888">
        <property type="term" value="F:glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106015">
        <property type="term" value="P:negative regulation of inflammatory response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045617">
        <property type="term" value="P:negative regulation of keratinocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010838">
        <property type="term" value="P:positive regulation of keratinocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000033">
        <property type="entry name" value="Exostosin-like glycosyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261">
        <property type="entry name" value="ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261:SF4">
        <property type="entry name" value="EXOSTOSIN LIKE GLYCOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 27" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="28">
        <location>
            <begin position="185"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="29">
        <location>
            <begin position="660"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="72"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00058348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00066812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00074807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00079618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00083226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="921" mass="104458" checksum="5E8D9FC4BD77831A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AX20</accession>
    <name>A0A673AX20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">si:dkey-202l22.3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AX20"/>
    <dbReference type="FunCoup" id="A0A673AX20">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035188.1">
        <property type="protein sequence ID" value="ENSSORP00005034271.1"/>
        <property type="gene ID" value="ENSSORG00005016200.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AX20"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001402">
        <property type="entry name" value="Prolrel_pep_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF15">
        <property type="entry name" value="PROLACTIN-RELEASING PEPTIDE RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01018">
        <property type="entry name" value="PRPRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="75"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="368" mass="40984" checksum="4F63F48845EEE961" modified="2020-06-17" version="1">ILFASGDMDIVMDEDFTINTNTSHEAGQENLDGSKSHGLNHSDPLDMFTGMELLLQFKSLFLPLYYLVVVVAGVGNSVLLACILADKKLHNATNFFIGNLAAGDLLMCLSCVPLTASYAFDSHGWAFGRPLCHLVPLLQCATVFASVLSLTAIAVDRYVVVAHPVRKRISLWGCGAVTLGIWLLSLTLAAPPSFYTRYVDLRPSKIDLVVCEEFWPNSSNLRLLYSCFILIASYMIPLLSVSVSYCAITVSLKHYTVPGEPYSSQQRWSKRRKKTFSLLVASVLAFALCWLPLQVLNLLLDLDPEFHIIDKRYINVLQVCCHLIAMSSACYNPFIYASLHSKVRMHLKRYLCSCHGRQEQTTGVTSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYP3</accession>
    <name>A0A673AYP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115434834</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AYP3"/>
    <dbReference type="FunCoup" id="A0A673AYP3">
        <property type="interactions" value="616"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034330.1">
        <property type="protein sequence ID" value="ENSSORP00005033423.1"/>
        <property type="gene ID" value="ENSSORG00005015826.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AYP3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050918">
        <property type="entry name" value="CNF-like_PLA2_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914:SF9">
        <property type="entry name" value="COILED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025511863" description="UPAR/Ly6 domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="9">
        <location>
            <begin position="19"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="9">
        <location>
            <begin position="104"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="198" mass="21346" checksum="A0730AD5097BE2EB" modified="2020-06-17" version="1">MHLFLLFSWIVLFPRAYTLKCNECITDITGKCNTYEQCPSETDQCTVVTGGVSLKSCASAHLCINGSLNTGRTRQVITSKCCNTDLCNDQLPPEPVAPRPNGKKCFTCDKFGDCIWNLDCVDNEDYCFSAAVDSAGQEMTRKGCTTTDLCLKAGKTELLENIAVEVSCCQGDYCNGAGSTGGSLLFLVVTVVSMVIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZQ7</accession>
    <name>A0A673AZQ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC115415980</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Member of the ionotropic glutamate receptor family, which plays a crucial role in synaptic organization and signal transduction in the central nervous system. Although it shares structural features with ionotropic glutamate receptors, does not bind glutamate as a primary ligand. Promotes synaptogenesis and mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis of cerebellar parallel fiber-Purkinje cell (PF-PC) synapses through the NRX1B-CBLN1-GRID2 triad complex. In the presence of neurexins and cerebellins, forms cation-selective channels that are proposed to be gated by glycine and D-serine. However, recent research disputes this ligand-gated cation channel activity. Cation-selective ion channel activity can be triggered by GRM1 in Purkinje cells.</text>
    </comment>
    <comment type="function">
        <text evidence="22">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14 22">Postsynaptic cell membrane</location>
            <topology evidence="14 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AZQ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005035550.1">
        <property type="protein sequence ID" value="ENSSORP00005034629.1"/>
        <property type="gene ID" value="ENSSORG00005016220.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZQ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051963">
        <property type="term" value="P:regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13730">
        <property type="entry name" value="PBP2_iGluR_delta_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000024">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000068">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000045">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000040">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 22" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 22" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6 22" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 22" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="591"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="823"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="23">
        <location>
            <begin position="427"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="24">
        <location>
            <begin position="437"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="734"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="20">
        <location>
            <position position="657"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="20">
        <location>
            <position position="684"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="748"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00056512"/>
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    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061411"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
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        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="998" mass="110997" checksum="A291F9FE3423E385" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B171</accession>
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            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035124.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="260" mass="29868" checksum="5DA937A8B4ADC31B" modified="2020-06-17" version="1">MSPTPLIYTLKYCITIFEHFFLQVRHDRIVPIFIINLVITDVLQLFCMIGLVAEPEYGRLYAALYHIYFFSLMASVGFIVCVALERYLVIAHPLWYHFSQPIKISVPASVVIWLLPFVYIIPDYLIAETFETDIIFATFHLLPLPFFIFFLAGAIKALSTAIHLTSIEKRRIIGTLVLPLIIYLLLFLPIVVSFLAKVNNRINEFSIMFIQFTPLANSFLYVLIRKGVLDKLLVCPCSKMDNDDLKTVSGSMKETKEICK</sequence>
</entry>
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    <accession>A0A673B2I1</accession>
    <name>A0A673B2I1_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">Transmembrane protein 50B</fullName>
        </recommendedName>
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        <name evidence="7" type="primary">tmem50b</name>
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        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="17792" checksum="337FB3279B0F6B2B" modified="2020-06-17" version="1">MAGFLDNFRWPECECIDWGERRNAVASIVAGVLFFTGWWIMIDAAVTYPSQEQMNHAFHTCGVFSTIAFFMINAVSNGQVRGDTYGEGCLGRTGARLWLFIGFMMMFGSLIASIWILFGGYVVPKKEVAPGLAVFFQNAFIFFSTLIYKFGRTEDLWG</sequence>
</entry>
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    <accession>A0A673B366</accession>
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        <submittedName>
            <fullName evidence="12">Trace amine-associated receptor 13c-like</fullName>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSSORG00005016482.1"/>
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        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="56"/>
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            <begin position="48"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="331" mass="37095" checksum="97B326BE2F874D59" modified="2020-06-17" version="1">MMEALEVASSKLCFPSLNNSCRTMSRSQLEAAVIYTLLSSTALMTVILNLVVIISISHFRQLHTTTNLLLLSLAVADFLMGFPQMLFQIFQTSGCWILGDIVCVIYYFCGFIVVSVSVGNMVLISIDRYVAICDPMLYPTKVTMKRVQFSIYLCWIFSALHASWILRDILKQPGRHSSCYGDCLVIINFAEGVVDLIATFFAPFIIITVLYMRVFVVAVSQARAIRSHVTSVTQQHPTVTAQKSEIKAAKTLGILVVVFLLFSSPYYCFTIAAESNLFDSSSGVVEIWLLSFNSCLNPIIYAFCYPWFRKSVKLIVTLQILQPDSREVSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3J4</accession>
    <name>A0A673B3J4_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Transmembrane protein 56-B-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="primary">LOC115411225</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="nucleotide sequence ID" value="XM_030123238.1"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="entry name" value="TLC-dom"/>
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        <property type="entry name" value="TLCD"/>
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        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 4"/>
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        <property type="entry name" value="TRAM_LAG1_CLN8"/>
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        <location>
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            <end position="23"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004141"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="263" mass="30426" checksum="A238EBC40E2B8F00" modified="2020-06-17" version="1">MDPFSQLILTISVTSFLTFQWLFHKVSPWMSIRISPSFLNLSDKQKVEWNSRTVSTFHALLVGIFCLYILWFDDAVNEDPVWGDPTLVKTNVAITTGYLISDLLLIFYYWKAIGDKFFVVHHLAALYAYYYVLGQGMLPYFANFRLLAEFSTPCVNQRWFFEVLGYPKSSRPNMANGVAMAVVFFMVRIAVMPVYYSRMYAVYGTEAFYLVPWGGRLAWICSSICLDIMNIMWMHKIARGCYKVLRSARRSKADTPQENGKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B970</accession>
    <name>A0A673B970_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSSORP00005038881.1"/>
        <property type="gene ID" value="ENSSORG00005018182.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B970"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="165" mass="19272" checksum="5860919DD8A9A3F5" modified="2020-06-17" version="1">QILRTFLEQDVLLERPLKMKSRGPKSRKRTVKGKRTVKGKSTVKGKRTVKGKRTVKGKRTVKGKRTVKGKRTVKGKRTVKGKRTVKGKRTVKGKRTVKGKRTVKGKRTRTVKGKRTVKRKRTVKGKRTCLYMFYIHKNTFIQHFCCETSCNYTRYLSINKQVWFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB84</accession>
    <name>A0A673BB84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BB84"/>
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        <property type="protein sequence ID" value="ENSSORP00005037877.1"/>
        <property type="gene ID" value="ENSSORG00005017768.1"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053025">
        <property type="entry name" value="Mito_ATP_Synthase-Asso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44873">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44873:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="51"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="128" mass="14436" checksum="D25F0BDD13EC0A98" modified="2020-06-17" version="1">MQLFSGVITGRSRWVKWQRSVRDWAPEPTGCPCSGPARAARPALEEVPDPSYYDILKVSPSATQSQIKSAYYKQSFIYHPDKNAGSEEAKQMFAEISEAYTVLGNITLRRKYDRGILKLPRTAHQTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEM1</accession>
    <name>A0A673BEM1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Bmi1 polycomb ring finger oncogene 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of rnf2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a PRC1-like complex. Homodimer. Interacts with cbx2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BEM1"/>
    <dbReference type="FunCoup" id="A0A673BEM1">
        <property type="interactions" value="435"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040060.1">
        <property type="protein sequence ID" value="ENSSORP00005039057.1"/>
        <property type="gene ID" value="ENSSORG00005018257.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BEM1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021549">
        <property type="term" value="P:cerebellum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17165">
        <property type="entry name" value="RAWUL_PCGF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16736">
        <property type="entry name" value="RING-HC_PCGF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000106">
        <property type="entry name" value="Polycomb complex protein BMI-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000082">
        <property type="entry name" value="Polycomb group ring finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825:SF21">
        <property type="entry name" value="POLYCOMB COMPLEX PROTEIN BMI-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING, POLYCOMB GROUP COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="230"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="299"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="343" mass="37946" checksum="A32233C3816D2611" modified="2020-06-17" version="1">MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKMCIVRYLETSKYCPICDVQVHKTKPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYADHPVDASNGSNEDRGEVADEDKRIITDDEIISLSIEFFDQSRMGGGVEDKQSKDQVANKRYLQCPAAMTVMHLRKFLRSKMDIPNTYQVEVMYEDEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPNCKKMKVSHAQQEGQNNASRSGPESDSASDKAGSPAGAPSTSTSSSLPSPGTPAQSPHAQLPHGPVNGTPAAANPPPPPPPLNPPRPFTQFSGGNGPNTGGGKARKVTLNGSSTTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFQ0</accession>
    <name>A0A673BFQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NACHT LRR and PYD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BFQ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005041661.1">
        <property type="protein sequence ID" value="ENSSORP00005040610.1"/>
        <property type="gene ID" value="ENSSORG00005018958.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BFQ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37955" checksum="D1BECB9146425B00" modified="2020-06-17" version="1">SNPSLLSINIFHCLTEMKDQTVHQEIQEFLKSENRSQKELSEIQCSALAYMLQISEEVLDELNLWKYNTSDEGRRRLIPAVRNCREAILSACRLSQTDCEVVASALKSNPSHLRLLDLSHNDLQDSGVKLASGLESPNCRLETLRLLTGCADLTLFFFSIYLLYYCSLSQISCSSVASALKSNPSHLIKLDLSGNELKDSSVKELCDFLQSPHCKLQDLRLHNCSLSQISCSSVASALKSNPSHLIKLDLSENKLKDSSVKELCDFLQSPHCKLQDLWLYKCSLSKISCSYLAAALKSNPSHLIKLDLCQNNLKDSDVKELEDLSPHYKLKSLWSVHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKK9</accession>
    <name>A0A673BKK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cystic fibrosis transmembrane conductance regulator</fullName>
            <ecNumber evidence="9">5.6.1.6</ecNumber>
            <ecNumber evidence="8">7.6.2.2</ecNumber>
            <ecNumber evidence="30">7.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">ATP-binding cassette sub-family C member 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Channel conductance-controlling ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="57">Multidrug resistance-associated protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="33">cAMP-dependent chloride channel</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="62" type="primary">LOC115435455</name>
    </gene>
    <organism evidence="62 63">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="62" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="62" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="62" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="50">
            <text>3',5'-cyclic AMP(in) + ATP + H2O = 3',5'-cyclic AMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="50">
            <dbReference type="Rhea" id="RHEA:66185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="55">
            <text>3',5'-cyclic GMP(in) + ATP + H2O = 3',5'-cyclic GMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="55">
            <dbReference type="Rhea" id="RHEA:66189"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>ATP + H2O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel.</text>
            <dbReference type="EC" id="5.6.1.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>an S-substituted glutathione(in) + ATP + H2O = an S-substituted glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19121"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:19122"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>cholate(in) + glutathione(in) + ATP + H2O = cholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29747"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="46">
            <dbReference type="Rhea" id="RHEA:66397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>dehydroepiandrosterone 3-sulfate(in) + ATP + H2O = dehydroepiandrosterone 3-sulfate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61364"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:61365"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="51">
            <text>glycochenodeoxycholate(in) + glutathione(in) + ATP + H2O = glycochenodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36252"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="51">
            <dbReference type="Rhea" id="RHEA:66409"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="53">
            <text>glycocholate(in) + glutathione(in) + ATP + H2O = glycocholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66400"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29746"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="53">
            <dbReference type="Rhea" id="RHEA:66401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="54">
            <text>glycodeoxycholate(in) + glutathione(in) + ATP + H2O = glycodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66380"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:82982"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="54">
            <dbReference type="Rhea" id="RHEA:66381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>glycoursodeoxycholate(in) + glutathione(in) + ATP + H2O = glycoursodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:132030"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:66417"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>hydrogencarbonate(in) = hydrogencarbonate(out)</text>
            <dbReference type="Rhea" id="RHEA:28695"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="47">
            <text>leukotriene B4(in) + ATP + H2O = leukotriene B4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57461"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>prostaglandin E1(in) + ATP + H2O = prostaglandin E1(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57397"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:66393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="48">
            <text>prostaglandin E2(in) + ATP + H2O = prostaglandin E2(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="48">
            <dbReference type="Rhea" id="RHEA:66389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="49">
            <text>taurochenodeoxycholate(in) + glutathione(in) + ATP + H2O = taurochenodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66412"/>
            <dbReference type="ChEBI" id="CHEBI:9407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="49">
            <dbReference type="Rhea" id="RHEA:66413"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="52">
            <text>taurocholate(in) + glutathione(in) + ATP + H2O = taurocholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66404"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36257"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="52">
            <dbReference type="Rhea" id="RHEA:66405"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>tauroursodeoxycholate(in) + glutathione(in) + ATP + H2O = tauroursodeoxycholate(out) + glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:132028"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:66421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>urate(in) + ATP + H2O = urate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16461"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:16462"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="56">Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); this interaction accelerates MRP4 internalization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Apical cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Basolateral cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Early endosome membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.6" evidence="9"/>
    <dbReference type="EC" id="7.6.2.2" evidence="8"/>
    <dbReference type="EC" id="7.6.2.3" evidence="30"/>
    <dbReference type="Ensembl" id="ENSSORT00005044265.1">
        <property type="protein sequence ID" value="ENSSORP00005043171.1"/>
        <property type="gene ID" value="ENSSORG00005019964.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BKK9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005260">
        <property type="term" value="F:intracellularly ATP-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18593">
        <property type="entry name" value="ABC_6TM_MRP4_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000027">
        <property type="entry name" value="ATP-binding cassette subfamily C member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000014">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000482">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030240">
        <property type="entry name" value="ABCC4_TMD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009147">
        <property type="entry name" value="CFTR/ABCC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF357">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01851">
        <property type="entry name" value="CYSFIBREGLTR"/>
    </dbReference>
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</entry>
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    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-{[(4-methoxybenzyl)(methyl)amino]methyl}-2-methylpropane-1,3-diol</text>
            <dbReference type="Rhea" id="RHEA:55764"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:139161"/>
            <dbReference type="ChEBI" id="CHEBI:139164"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>11,12-epoxy-(5Z,8Z,14Z)-eicosatrienoate + H2O = 11,12-dihydroxy-(5Z,8Z,14Z)-eicosatrienoate</text>
            <dbReference type="Rhea" id="RHEA:44044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:76625"/>
            <dbReference type="ChEBI" id="CHEBI:84031"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:44045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = glycerol + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:26133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>8,9-epoxy-(5Z,11Z,14Z)-eicosatrienoate + H2O = 8,9-dihydroxy-(5Z,11Z,14Z)-eicosatrienoate</text>
            <dbReference type="Rhea" id="RHEA:44048"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:84025"/>
            <dbReference type="ChEBI" id="CHEBI:84032"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:44049"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cis-stilbene oxide + H2O = (1R,2R)-hydrobenzoin</text>
            <dbReference type="Rhea" id="RHEA:23900"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:50004"/>
            <dbReference type="ChEBI" id="CHEBI:50014"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:23901"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
            <topology evidence="6">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Microsome membrane</location>
            <topology evidence="5">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 19">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.9" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A673BML7"/>
    <dbReference type="Ensembl" id="ENSSORT00005044100.1">
        <property type="protein sequence ID" value="ENSSORP00005043010.1"/>
        <property type="gene ID" value="ENSSORG00005019868.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BML7"/>
    <dbReference type="OrthoDB" id="7130006at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF78">
        <property type="entry name" value="EPOXIDE HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="9" id="KW-0216">Detoxification</keyword>
    <keyword evidence="13 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0492">Microsome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="21">
        <location>
            <begin position="67"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="20">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="20">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR001112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="468" mass="54071" checksum="A80547A9B2D40429" modified="2020-06-17" version="1">MEKLHILKEAFLGLDVVQQRLLIGSAVAAGGILACMVHKRRQVKTIPLGEGWWGAGQKSLSKDDNIYSFRVETSDKDIEDLYERIDRTRYTDPLEDSGFHYGFNSTYLKKVVSYWRHEFDWKKQVEMLNKYPHFKTKIEGLDVHFIHVRPPHRENQKVLPLMLVHGWPGCFYEFYKIIPLLTENQDGVAFEIICPSIPGYGFSEAPHKQGFDSLSAARIFLTLMERLGFSQFYLQGGDWGSLITTNMAQMKPQCVKGLHLNMFLSRRGFKVLLGLMFGRYLPFLVGLTWEDVRRLFPYFEKNVWEILRETGYLHIQATKPDTAGCGVNDSPVGLAAYILEKFSTWTDLENRALEDGGLERKFNLDELLTVIMIYWTSGSIVSSMRFYKENTKRNFDNRVDQKTGIFVPTGLAAFPNELMHCPQAWAQIRYKNIYSYTFMSRGGHFAAFEEPKLLADDILQFVKKVEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BPN7</accession>
    <name>A0A673BPN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Insulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115427514</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Heterodimer of a B chain and an A chain linked by two disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the insulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BPN7"/>
    <dbReference type="Ensembl" id="ENSSORT00005045821.1">
        <property type="protein sequence ID" value="ENSSORP00005044696.1"/>
        <property type="gene ID" value="ENSSORG00005020561.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BPN7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04367">
        <property type="entry name" value="IlGF_insulin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.100.10">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022352">
        <property type="entry name" value="Ins/IGF/rlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004825">
        <property type="entry name" value="Insulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016179">
        <property type="entry name" value="Insulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036438">
        <property type="entry name" value="Insulin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022353">
        <property type="entry name" value="Insulin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11454:SF9">
        <property type="entry name" value="INSULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11454">
        <property type="entry name" value="INSULIN/INSULIN GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00049">
        <property type="entry name" value="Insulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00277">
        <property type="entry name" value="INSULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00276">
        <property type="entry name" value="INSULINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00078">
        <property type="entry name" value="IlGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56994">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00262">
        <property type="entry name" value="INSULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="7 10" id="KW-0372">Hormone</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Insulin-like" evidence="11">
        <location>
            <begin position="7"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="52" mass="5976" checksum="D0287CF2738C0534" modified="2020-06-17" version="1">MARVPWTHLCGSHLVDALYFVCGDRGFFVKRGIVEQCCHKPCSIYHLEAYCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTI6</accession>
    <name>A0A673BTI6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xylulose kinase</fullName>
            <ecNumber evidence="4">2.7.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">xylb</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Phosphorylates D-xylulose to produce D-xylulose 5-phosphate, a molecule that may play an important role in the regulation of glucose metabolism and lipogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-xylulose + ATP = D-xylulose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17140"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.17" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673BTI6"/>
    <dbReference type="Ensembl" id="ENSSORT00005046226.1">
        <property type="protein sequence ID" value="ENSSORP00005045090.1"/>
        <property type="gene ID" value="ENSSORG00005019415.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BTI6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004856">
        <property type="term" value="F:D-xylulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005997">
        <property type="term" value="P:xylulose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07776">
        <property type="entry name" value="ASKHA_NBD_FGGY_SpXK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000118">
        <property type="entry name" value="Xylulose kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042024">
        <property type="entry name" value="D-XK_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF57">
        <property type="entry name" value="XYLULOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0859">Xylose metabolism</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="6">
        <location>
            <begin position="192"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="7">
        <location>
            <begin position="352"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="543" mass="59537" checksum="D4E81AEEE8BAD674" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVQ0</accession>
    <name>A0A673BVQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat domain 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ankrd9</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029982001.1">
        <property type="nucleotide sequence ID" value="XM_030126141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BVQ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005045767.1">
        <property type="protein sequence ID" value="ENSSORP00005044642.1"/>
        <property type="gene ID" value="ENSSORG00005020552.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413354"/>
    <dbReference type="InParanoid" id="A0A673BVQ0"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30829" checksum="69EF8597F3E0E65A" modified="2020-06-17" version="1">MPWLVSSQLSPLSLSPSQRHCERTAFAFYCAVRDQEPVWVLEDMRTMEVFCWDGGRPRAFEPSDALLYSLVHDHRDYARYLLHRFSVSALRAPCCSFCFSSGGSPHLSVAVRYNRLDILTLMAEVLQVLPEAERRAYLDRCSGCFHLSDGGKTAVQLGVELVRPDCLLLLLVHGARPDGLEAALRRLEAAEGAELRHARRCLDLLLLFMPEPPTGPPCLKEEPQRWQSLLGNEVFDWLSGSAPPSLLLQALRTLARSVPGQIADLPDRLHPQSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BWL7</accession>
    <name>A0A673BWL7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">septin4a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BWL7"/>
    <dbReference type="Ensembl" id="ENSSORT00005046107.1">
        <property type="protein sequence ID" value="ENSSORP00005044972.1"/>
        <property type="gene ID" value="ENSSORG00005020486.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BWL7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000064">
        <property type="entry name" value="Septin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="12">
        <location>
            <begin position="101"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="8">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006698-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="453" mass="52192" checksum="A7AA1BF6626AFD40" modified="2020-06-17" version="1">MCQSPCTSLPNDPTGTPPLPLPLPHPTGTPPLPRTGRDVQSLPMKRQLVSQGSLDPPLSPASRPHSPWGFFDPYDSPEDQDKEYVGFAMLPNQVHRKTVKKGFTFTLMVAGESGLGKSTLINSLFLTDLYKERKVPNAEERINKTVDIVKHTVSIEEKGIKLRLTIIDTPGFGDAVNNTESWKPLEDYIDQQFEQYFRDESGLNRRNIQDNRVHCCLYFISPFGHGLRPLDVECMKALHEKVNIVPILAKADSLTAAEMRRKKMKIREEIKQFGINIYQFPEYDSDEEADFKRQDQILKDSIPFAVIGSNIQVESKGRRVRGRIYPWGMVEVENPVHSDFLLLRNMLVRTHMQDLKDVTRESHYENYRANCIQNMTRMVVLERKRSLREKFREGSGVDFPLPLAVVDTEKERLIFEKDEELRKMQEVLERIQEQMLNTQHESLVMRQPAATIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXK6</accession>
    <name>A0A673BXK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">zdhhc15b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673BXK6"/>
    <dbReference type="FunCoup" id="A0A673BXK6">
        <property type="interactions" value="928"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048871.1">
        <property type="protein sequence ID" value="ENSSORP00005047691.1"/>
        <property type="gene ID" value="ENSSORG00005021774.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BXK6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="130"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="342" mass="40096" checksum="AC4C938AB8838DA2" modified="2020-06-17" version="1">MRAEVRWNMALSRGLRCCQRVFSWIPVLIITSVVLWSYYAYVFELCLFTIPNTFEKVAYLLVFHVCFVMFSWTYWKSIFTPPATPCKKFQLSYSDKQRYEMEERPDAQKQILVEIAKKLPIFTRAQSGAIRFCDRCQVLKPDRCHHCSVCETCVLKMDHHCPWVNNCVGFSNYKFFLLFLSYSMLYCIFIAATVFQYFLKFWVGDLPNGSAKFHVLFLMFVALMFFVSLMFLFGYHCWLVAKNRSTLEAFSAPVFVSGPDRNGFNVGVRKNLQQVFGENRRLWFIPVFTSQGNGHFFPLKNRSESRNPLLANEEMWEESEDCSEEGSLVEDQDPSVTIEMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXU1</accession>
    <name>A0A673BXU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-galactosidase</fullName>
            <ecNumber evidence="5">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115431698</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673BXU1"/>
    <dbReference type="Ensembl" id="ENSSORT00005046067.1">
        <property type="protein sequence ID" value="ENSSORP00005044933.1"/>
        <property type="gene ID" value="ENSSORG00005020649.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BXU1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000049">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000036">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026283">
        <property type="entry name" value="B-gal_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048912">
        <property type="entry name" value="BetaGal1-like_ABD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21317">
        <property type="entry name" value="BetaGal_ABD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006336">
        <property type="entry name" value="B-gal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="7">
        <location>
            <begin position="13"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase 1-like first all-beta" evidence="8">
        <location>
            <begin position="383"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase galactose-binding" evidence="9">
        <location>
            <begin position="509"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="605" mass="69017" checksum="0BAEF756E480270E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C687</accession>
    <name>A0A673C687_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphorylase kinase</fullName>
            <ecNumber evidence="3">2.7.11.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">phkg2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + phosphorylase b = 2 ADP + phosphorylase a.</text>
            <dbReference type="EC" id="2.7.11.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.19" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673C687"/>
    <dbReference type="FunCoup" id="A0A673C687">
        <property type="interactions" value="1137"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049961.1">
        <property type="protein sequence ID" value="ENSSORP00005048769.1"/>
        <property type="gene ID" value="ENSSORG00005022200.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C687"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004689">
        <property type="term" value="F:phosphorylase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000138">
        <property type="entry name" value="Phosphorylase b kinase gamma catalytic chain, liver/testis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000149">
        <property type="entry name" value="phosphorylase b kinase gamma catalytic chain, liver/testis isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002291">
        <property type="entry name" value="Phosph_kin_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01049">
        <property type="entry name" value="PHOSPHBKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="381" mass="44086" checksum="545E99166DFECA6C" modified="2020-06-17" version="1">MTKDIVVGDELPDWVGAKEFYQKYDPKEVIGRGVSSVVRRCVHRHTGQELAVKIIEITAEKMTAQQLEEVKTSTLKEIQVLNMVKGHSSIITLIDSYESATFIFLVFDLMRRGELFDYLTEKSMMRALLEAVQYLHSLNIIHRDLKPENILLDDQGHIKLSDFGFSVQLQPGEKLRELCGTPGYLAPEILKCSMDETHQGYGKEVDLWACGVILFTLLAGSPPFWHRKQMLMLRMIMEGRYQFSSPEWDDRSDTVKDLISRLLVVDPATRLTAEQALAHSFFRQYQREDVRLFSPRKSFRVLIVTVQACIRMYSRYRRVRPLTREVLARDPYSLRGVRKLIDGCAFRIYGHWVKKGEQQNRAALFQNTAKIMLLGLEDFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C773</accession>
    <name>A0A673C773_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hippocalcin-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hpcal1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C773"/>
    <dbReference type="FunCoup" id="A0A673C773">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049869.1">
        <property type="protein sequence ID" value="ENSSORP00005048677.1"/>
        <property type="gene ID" value="ENSSORG00005022167.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C773"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF79">
        <property type="entry name" value="HIPPOCALCIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
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            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="193" mass="22363" checksum="74D05044C0A39B3A" modified="2020-06-17" version="1">MGKQNSKLRPEVLNDLRENTEFTDHELQEWYRGFLKDCPTGHLTVEEFKKIYANFFPYGDASKFAEHVFRTFDTNGDATIDFREFIIALSVTSRGGLEQKLRWAFSMYDLDGNGYISRAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDIDNDGRLSLEEFIKGAKSDPSIVRLLQSDQGASRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7C0</accession>
    <name>A0A673C7C0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine zipper protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">luzp1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029982686.1">
        <property type="nucleotide sequence ID" value="XM_030126826.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029982687.1">
        <property type="nucleotide sequence ID" value="XM_030126827.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029982689.1">
        <property type="nucleotide sequence ID" value="XM_030126829.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029982690.1">
        <property type="nucleotide sequence ID" value="XM_030126830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C7C0"/>
    <dbReference type="Ensembl" id="ENSSORT00005049327.1">
        <property type="protein sequence ID" value="ENSSORP00005048138.1"/>
        <property type="gene ID" value="ENSSORG00005021980.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413764"/>
    <dbReference type="CTD" id="7798"/>
    <dbReference type="InParanoid" id="A0A673C7C0"/>
    <dbReference type="OrthoDB" id="9946011at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050719">
        <property type="entry name" value="Cortactin-Actin_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166">
        <property type="entry name" value="FILAMIN/GPBP-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166:SF7">
        <property type="entry name" value="LEUCINE ZIPPER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="396"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="609"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="757"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="878"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="972"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1009"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="9"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="285"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="523"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="614"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="675"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="757"/>
            <end position="782"/>
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        <location>
            <begin position="881"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="920"/>
            <end position="930"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="973"/>
            <end position="987"/>
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            <begin position="1042"/>
            <end position="1053"/>
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        <location>
            <begin position="1107"/>
            <end position="1116"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048138.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1136" mass="125056" checksum="1DB3200872191D84" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7E8</accession>
    <name>A0A673C7E8_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">Fibronectin type III domain containing 10</fullName>
        </recommendedName>
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        <name evidence="4" type="primary">fndc10</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Neoteleostei</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <property type="component" value="Chromosome 5"/>
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            <begin position="1"/>
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            <begin position="304"/>
            <end position="329"/>
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    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="350" mass="38552" checksum="8591099910D433A7" modified="2020-06-17" version="1">MKRQQPPSLLALSALLLCTLQQTAASVRSDRNSSASSSSSSSLSSLSSTVSSQVSSRKPQDWQKLTFNYGNQSLTSRPSPKLKEASGHVNSFSFNTSRGNITTVISKRSGRTPSLSPEEEVSPLCAYRVIEGGIGGQLCFRDTLFGYKCQEGECRTVVSYGSLVANILINGSVLLQWTHEGESTMSEQNTTKETVAGQKNGRDPKTNSTRVETLKSRAVFSSRRHRRGGYELSCWWNGSYTQFECAGVHLASGCRDFLLTELHENIPYRLCLRSLEHSTHSPAQTTDRRDCVEFTLPPSGMQDIVIAMTTVGGAICVMLVIICLLVAYITENIMSPTTQHTYSYRTHSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7U6</accession>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:zinc ion binding"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CH_CAMSAP_2"/>
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        <property type="entry name" value="PH_Vav"/>
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        <property type="entry name" value="PKC_DAG/PE"/>
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    <dbReference type="PANTHER" id="PTHR45818:SF4">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR VAV2"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN VAV"/>
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        <property type="entry name" value="SOS1_NGEF_PH"/>
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        <property type="entry name" value="SH2DOMAIN"/>
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        <property type="entry name" value="C1"/>
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    </dbReference>
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        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
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        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="16">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="15">
        <location>
            <begin position="194"/>
            <end position="372"/>
        </location>
    </feature>
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        <location>
            <begin position="401"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="514"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="651"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="794"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="845" mass="97921" checksum="E43F712A4C95CCDA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9K2</accession>
    <name>A0A673C9K2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Claudin-8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cldn8.2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C9K2"/>
    <dbReference type="FunCoup" id="A0A673C9K2">
        <property type="interactions" value="414"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051905.1">
        <property type="protein sequence ID" value="ENSSORP00005050690.1"/>
        <property type="gene ID" value="ENSSORG00005022970.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C9K2"/>
    <dbReference type="OrthoDB" id="8819159at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="33264" checksum="F295579426B034C0" modified="2020-06-17" version="1">MASYTAYSGYTKPPQSYAGSYYDEKQAQAYTDYQDSVYEEKKRIEKKDRREAICCEVVALVIGFIGLIGVAAVTGLPMWKVTAFIEENIIVMETRWEGLWMNCYRQANIRMQCKVYDSLLFLPPDLQAARGLMCASVALTSFALIVSAVGMKCTKVVDHRARTKHIVLVAGGCLFLMGCVTTLIPVSWTGHVIIRDFYNPLLIDAQRRELGEALYIGWVTGAILFTAGVILLCRHAPRTQDPDERIAYNPGQNIYQPGYSYQPNTYQPYTYQPAYSYQPPYSAPPPGSVAYTPSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CA28</accession>
    <name>A0A673CA28_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CA28"/>
    <dbReference type="FunCoup" id="A0A673CA28">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053673.1">
        <property type="protein sequence ID" value="ENSSORP00005052426.1"/>
        <property type="gene ID" value="ENSSORG00005023623.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CA28"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="135"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="273" mass="30884" checksum="254432F8DAF848BD" modified="2020-06-17" version="1">FILYKMRQQDRDRFLLEQCFSFHTLGLISANIGDSVSLQCFFPSGTWFHWYKQTVGQMPRLISTCYSYDSVGVFDSEFQNNPRFTLDTTKEHCHLNISDLRVSDSATYFCINSQSSKVTFLESITVNVKASDFNIQTSVHQSESESVHPGDSVTLNCTVHTGTCDGQHSVYWFKKSEDPQPGIIYTHGGTNHQCEKKNDTQTHTCVYNLPILDVDYPHAGTYYCAVASCGHVLFGNGTTVNFKYFKVVDPLMSHILLCLLCIYFCVVVASNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAE3</accession>
    <name>A0A673CAE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sec1 family domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Syntaxin-binding protein 1-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115414145</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">Golgi stack membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CAE3"/>
    <dbReference type="Ensembl" id="ENSSORT00005052218.1">
        <property type="protein sequence ID" value="ENSSORP00005051000.1"/>
        <property type="gene ID" value="ENSSORG00005023055.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CAE3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000002">
        <property type="entry name" value="Sec1 family domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000002">
        <property type="entry name" value="sec1 family domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="570" mass="64338" checksum="1090495442E1F456" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAJ1</accession>
    <name>A0A673CAJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cldn7a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CAJ1"/>
    <dbReference type="FunCoup" id="A0A673CAJ1">
        <property type="interactions" value="941"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053834.1">
        <property type="protein sequence ID" value="ENSSORP00005052586.1"/>
        <property type="gene ID" value="ENSSORG00005023689.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CAJ1"/>
    <dbReference type="OrthoDB" id="10025519at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01385">
        <property type="entry name" value="CLAUDIN14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="213" mass="22524" checksum="3719992AD82CE3AE" modified="2020-06-17" version="1">MANSGIQLLGFFLSLIGIVGLIIGTILPQWKMSAYIGDNIITAVAMYQGLWMSCAFQSTGQLQCKIYDSILQLDSSLQATRALMIVGIIVSIAGLGVACMGMKCTTCGAGDKLRKSRIAMTGGIILLVGGLCAIVACSWFAHNVIRAFYNPYTPVNTKFEFGAAIFIAWGGSLLDVLGGAMLAASCPRKKQVSKYPSMASSRSGPPSSAKEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAN9</accession>
    <name>A0A673CAN9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">G protein-coupled receptor 65</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CAN9"/>
    <dbReference type="Ensembl" id="ENSSORT00005051025.1">
        <property type="protein sequence ID" value="ENSSORP00005049822.1"/>
        <property type="gene ID" value="ENSSORG00005022605.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CAN9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF10">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="48"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="315" mass="35342" checksum="078F51AE16823604" modified="2020-06-17" version="1">MHFHLITQTNTDNHSNNQLNSSCTEEEWMPIKVLPVFMWVITGLGIVLNILVVLVFSWMLMRNGSNMAVYLVSLSISDLLYIVSLPVWIELSLRRPVGCGLCSVTAVVMNNSFYVGSGLLCCISVDRYLAVVYPLHFHWVREVRTAAVVWALQAFSSSTLCMEKIPMSHETAHLALTRVILGFFVPFVIMSFCFQQIMYSLKRSTSIVAEERRKVGRLLLLLLLAYVVAFVPYQTVMLLRAVLEPRTDTSAFQLKAAYLVTVATTTLNSVLDPIIYCLISESAQGEIKKLIEKGRQVCSIVTIETPSSPTTLINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCR6</accession>
    <name>A0A673CCR6_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">zgc:92140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF4612"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14974">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 1700025G04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14974:SF3">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 1700025G04 GENE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15389">
        <property type="entry name" value="DUF4612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="113" mass="12927" checksum="98944EF23150F33D" modified="2020-06-17" version="1">MGCTSAKQVSAVPNDEEGRGKAYSNGDLFTDEYKMKGVEEVKYMRGEENRVNARNQENLEKSNVQYRGKQQKEVATANKSNIHTSESQQEFFRMLDEKIEKGKDYCSEEEDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CEV3</accession>
    <name>A0A673CEV3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">von Willebrand factor A domain containing 10, tandem duplicate 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">vwa10.2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CEV3"/>
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        <property type="protein sequence ID" value="ENSSORP00005051252.1"/>
        <property type="gene ID" value="ENSSORG00005023171.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NG37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905:SF18">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING 10, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23560">
        <property type="entry name" value="GBD_Hemicentin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025453668" evidence="5">
        <location>
            <begin position="23"/>
            <end position="653"/>
        </location>
    </feature>
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        <location>
            <begin position="165"/>
            <end position="332"/>
        </location>
    </feature>
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        <location>
            <begin position="343"/>
            <end position="515"/>
        </location>
    </feature>
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        <location>
            <begin position="534"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="653" mass="70343" checksum="B92802A06752F098" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CF09</accession>
    <name>A0A673CF09_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CF09"/>
    <dbReference type="FunCoup" id="A0A673CF09">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053949.1">
        <property type="protein sequence ID" value="ENSSORP00005052700.1"/>
        <property type="gene ID" value="ENSSORG00005023743.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CF09"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
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        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF39">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY S MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="15">
        <location>
            <begin position="17"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="190"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="464" mass="52951" checksum="6339872DDCDCB1EA" modified="2020-06-17" version="1">MAYRQVLHRHSDDDCFINVNVGGFKQRMEHDVVKRFPQTRLGRLLCCSSKEAILELCDDFSPSEMEYYFDRNPLFFCYVLNFYLTGKIHLVDGLCVISFVQEIEYWGIKERHLDMCCSNRFLELMDIVKNKDWDERSDEQQLNMSESSSIAELSASEDDMEMFEGTWCADVRRNIWIHLENPGYSTSAKAMAVASLSMVIVSVIAMCVHSMPDFHQVDHNDREIENPVLAFFENLCVLFFSAEFVLRLVVAPSAKKFLCSPLNIIDLMSVMPFYVTMLWMYLDEGESAELENVGKVVQILRLMRVFRILKLARHSSGLRSLGATLRHSSREVGMLVLFLSVGISMFSALVCFVEGDSEESTLQNMPVGWWWATISMTTVGYGDTFPVTFAGKVIGTLCIVCGLLIVALPITNIFNKFSKFYQRQKHIDLIRLLSCRVLTAAGKQKSKNHCCTGKNEMCTQVPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CH05</accession>
    <name>A0A673CH05_9TELE</name>
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        <submittedName>
            <fullName evidence="14">Si:dkey-165a24.9</fullName>
        </submittedName>
    </protein>
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        <name evidence="14" type="primary">si:dkey-165a24.9</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A673CH05"/>
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        <property type="protein sequence ID" value="ENSSORP00005052579.1"/>
        <property type="gene ID" value="ENSSORG00005023684.1"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 132-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="P2YPURNOCPTR"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="343" mass="38424" checksum="35B460D36D3C71D4" modified="2020-06-17" version="1">PCGVLTPKLKNTDMSNDSCNLPLNTDTLGLTCIYGVIFSLGLPSNLLSLWGLYHLGRSGTGGCQLVYILNLLLSDLLQLLTLPLWILYLQGGHRWPYGQLTCELVGYVFYVNVYASVMFLCLIALDRCLAIVYPLSSRRVRTVRVAAVMGSAVWTLTFLFCLCGLLPSVFDSDRLLCLEQYPVSPRYAHFKITTVALGFLLPLNILYLSLHSELRSPSLSDHERHKIVGILVVITINFIAVFGPYHLVGGYRFVSLLLTDKPCGLERSIFLVYRLCYGLTSLNTLLDPLFYIFLCPDARLELQRSLPCLGRGHNTHKTMTLSPRAHRVTQRQNETGHNNLLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLC2</accession>
    <name>A0A673CLC2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WAPL cohesin release factor a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">wapla</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
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    <dbReference type="AlphaFoldDB" id="A0A673CLC2"/>
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        <property type="gene ID" value="ENSSORG00005024240.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 15"/>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="WAPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022771">
        <property type="entry name" value="WAPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012502">
        <property type="entry name" value="WAPL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100:SF13">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07814">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAPL" evidence="3">
        <location>
            <begin position="493"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
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    </feature>
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            <begin position="952"/>
            <end position="972"/>
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        <location>
            <begin position="12"/>
            <end position="25"/>
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        <location>
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        <location>
            <begin position="114"/>
            <end position="181"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="226"/>
        </location>
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        <location>
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        <location>
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        </location>
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        <location>
            <begin position="348"/>
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        </location>
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        <location>
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            <end position="453"/>
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    </feature>
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            <begin position="958"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006854"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51271"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1061" mass="117264" checksum="281BE2D0102B0FB4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLJ1</accession>
    <name>A0A673CLJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rbm34</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM RBM34 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030014589.1">
        <property type="nucleotide sequence ID" value="XM_030158729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CLJ1"/>
    <dbReference type="FunCoup" id="A0A673CLJ1">
        <property type="interactions" value="575"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054753.1">
        <property type="protein sequence ID" value="ENSSORP00005053497.1"/>
        <property type="gene ID" value="ENSSORG00005024055.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436008"/>
    <dbReference type="CTD" id="23029"/>
    <dbReference type="InParanoid" id="A0A673CLJ1"/>
    <dbReference type="OrthoDB" id="442677at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12394">
        <property type="entry name" value="RRM1_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12395">
        <property type="entry name" value="RRM2_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034221">
        <property type="entry name" value="RBM34_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="164"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="267"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="338"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="417" mass="46762" checksum="C01486F495A8AF09" modified="2020-06-17" version="1">MKKKLQQSVEASSGQQSADYVVGQVSGSLFQKSSTSSDSLSALFSTAGPAASELFKPAPKPVKTSVEVKEQQRETPEVKGQPSQTTAKKKKPPKEKSTADQNLEKREVSLQNADEDETSTMNNKKKKKKRTASEAGQVNDPEYWVAKRQRLKASKEEEELKRKRTVFVGNLPISCTKKTLRNLFRDKGSIESIRFRSVVREDPSMSRKVAAIMRKVHPKKQSVNAYMVFRDQEGVAKALERNGTEIEKDFYIRVDRVTDTSTHDHKRSVFVGNLPFEVSELMFRRHFEECGGVEAVRLVRDQNSGMGKGFGYVLFESADSVQLALKLDGSKLDGRSIRVKRSVKKEKQKNKPGDKERTGRMGHGPTKGVTRGGFKSPKKFTGNRSSSSFKGEMADPQKKPKKKPQKKKVKPKKAVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CP08</accession>
    <name>A0A673CP08_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115418264</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RBR family. RNF14 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673CP08"/>
    <dbReference type="Ensembl" id="ENSSORT00005057303.1">
        <property type="protein sequence ID" value="ENSSORP00005056010.1"/>
        <property type="gene ID" value="ENSSORG00005024955.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CP08"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20341">
        <property type="entry name" value="BRcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16628">
        <property type="entry name" value="RING-HC_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000137">
        <property type="entry name" value="RanBP-type and C3HC4-type zinc finger-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031128">
        <property type="entry name" value="RNF14_RING-HC_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RWD" evidence="13">
        <location>
            <begin position="10"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="188"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="416" mass="47197" checksum="48661E766CC705AD" modified="2020-06-17" version="1">MNSDSEEQEDELLALCSIYDSDEFIRNESKAAGEFRVSVELRKDFTVTLTEGETLTQYDLSFLPPLHLTFELPEDYPSTSPPSFTLTCCWLTQAQLSALGTQLIDLYQSTGGAVVLFSWVQFLKEEALGFLDIQTRLEIPSIEHSALFSNKENQTANLSTLILTPAQALLSQLLIHDATQKEKVFYTTVFDCGVCFMCWLGSECVQIPQCGHVFCRSCLTEFCRLQITEGNVRDITCPQADCDATPTPAQVRSLVGEELFNRFDRLLLQSTLDYMSDVSYCPRRSCGSVVIVEKSGSMAMCSVCSYAFCVLCKKTYHGTEDCQKMNKDRRRLLESRYGRCNLVVTMESRLSQEWIAVTCKNCPYCFSRIEKNGGCNRMTCSRCSRQFCWACLTKLTYENNHQHFSHDSPCTIWAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQ21</accession>
    <name>A0A673CQ21_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Inosine-uridine preferring nucleoside hydrolase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:ch211-201h21.5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CQ21"/>
    <dbReference type="FunCoup" id="A0A673CQ21">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057671.1">
        <property type="protein sequence ID" value="ENSSORP00005056375.1"/>
        <property type="gene ID" value="ENSSORG00005025086.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CQ21"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02649">
        <property type="entry name" value="nuc_hydro_CeIAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="326" mass="36457" checksum="B43B7BD95637BD14" modified="2020-06-17" version="1">MAKKLVIIDTDCGIDDAQAIMMALAAPNIQVLGVTCVFGNADIDNVCQNVLRVLSVCEREQIPVFRGARGPLVGANKPHSDHFGTDGLGDVIKDKDPQWEKKIQREHAVDAMIRLVSEHQNQVSLVALGPLTNLALAVRLDPDFPQKLKDLYIMGGNMEGKGNVTLCAEFNFLIDVESAYIVLEEFLCPTFIATWEYSCRNALPWEFFEELINQDAPAARFMKMITSKCWAYSKETMRNKRDIYFGPGFVSYDAYAMAACIDSSLVTERIECPVRVELQGSIARGMMALDRTNELKKSHSVFVLTKCDIDKFSQLLMASLRQPCNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRV1</accession>
    <name>A0A673CRV1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 56-B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tlcd4b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CRV1"/>
    <dbReference type="FunCoup" id="A0A673CRV1">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058348.1">
        <property type="protein sequence ID" value="ENSSORP00005057045.1"/>
        <property type="gene ID" value="ENSSORG00005025330.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CRV1"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF49">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 4-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="41"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="269" mass="30067" checksum="77F169D084EB8560" modified="2020-06-17" version="1">MEMRELTVVAGSFVGFQLLFSVASPRLSSAITPGYGRLPPTKLTEWNSRLVSTVHALIVGLFCLYILWFDDAVNANPVWGDPSLVKLNVAVTCGYLLYDLVLLACNWSTMGDSFFVCHHLAALYAYGYVLTRGVLPYFANFRLISELSTPFVNQRWFFEALKYPRSHRMVVLNGVAMAVVFFLVRIAVMPSYWVSVFATFGTPDFEQLGLGAQVAWITSCIALDILNTIWMYKITRGCYKVLTGSGGRKIMEGADSVSDKKDHINNHTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CTV6</accession>
    <name>A0A673CTV6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spermatogenesis associated serine rich 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115422753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CTV6"/>
    <dbReference type="Ensembl" id="ENSSORT00005058131.1">
        <property type="protein sequence ID" value="ENSSORP00005056829.1"/>
        <property type="gene ID" value="ENSSORG00005025257.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CTV6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009816">
        <property type="entry name" value="SPATS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623:SF11">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07139">
        <property type="entry name" value="SPATS2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="49803" checksum="5D9FDFBF219EF6B0" modified="2020-06-17" version="1">MNKRVYVNVSWFHDTSGVVFDTRSKMVMSQGGTAERMKEKISAVRAVVPNKSNNEIVLVLQHFENCVDKAVQAFLEGKRTSQCHCKYFNIHQPKKKKKPKPQAEASAEPAPPEAIQESKDEINGFHANGSVMDGESLDSLSEQLDSASLDAAELDSEPASSETTGNHSSDLKTIMKYRKPANVMSNMYISAPNIDRSVKDLQRCTASLTRYRMVVKEEMDSSIKRMKQTFAELQSCLMDREVTLLGEMDKVKAEAMMILDARQKRAEELRRLTDQSASMSEEQLTELRADIKHFVSERKYDEDLGKAVRFTFDLEPMKTSISAFGSVYHPRTGYSNRSRCSSTSSSVTRHTSVPALTDFSFSPQIFQGNRRTFQGQGYHSGGQRYNGSSYHDRNAGRGNYRNQSDSGYSGPSSQHSNNSRGPAHPDRAPHHNGLPQRPPRTHCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CVC1</accession>
    <name>A0A673CVC1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Synaptotagmin binding, cytoplasmic RNA interacting protein, like</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CVC1"/>
    <dbReference type="Ensembl" id="ENSSORT00005057197.1">
        <property type="protein sequence ID" value="ENSSORP00005055908.1"/>
        <property type="gene ID" value="ENSSORG00005024914.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CVC1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21066">
        <property type="entry name" value="NURR_hnRNPQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12483">
        <property type="entry name" value="RRM1_hnRNPQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12489">
        <property type="entry name" value="RRM2_hnRNPQ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM3_hnRNPQ"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein q isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000024">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein q isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000027">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein q isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041337">
        <property type="entry name" value="hnRNP_Q_AcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006535">
        <property type="entry name" value="HnRNP_R/Q_splicing_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034544">
        <property type="entry name" value="hnRNPQ_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034548">
        <property type="entry name" value="hnRNPQ_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01648">
        <property type="entry name" value="hnRNP-R-Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18360">
        <property type="entry name" value="hnRNP_Q_AcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0492">Microsome</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="12 17" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="19">
        <location>
            <begin position="206"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="19">
        <location>
            <begin position="287"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="19">
        <location>
            <begin position="382"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="541"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="555"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="591" mass="66202" checksum="D2097121096175AD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CYL1</accession>
    <name>A0A673CYL1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Synaptotagmin XV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">syt15</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CYL1"/>
    <dbReference type="Ensembl" id="ENSSORT00005058583.1">
        <property type="protein sequence ID" value="ENSSORP00005057268.1"/>
        <property type="gene ID" value="ENSSORG00005025415.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CYL1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF234">
        <property type="entry name" value="SYNAPTOTAGMIN-15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="174"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="314" mass="35444" checksum="AC1A1D06B19980EB" modified="2025-10-08" version="2">MPPRFVTQNHKDLQNTEGKEKEVEMEMKMEAQRDILAHRGSLSVRSWYPVGTVLAGLYSVSPVAPPPGMATRLCFSVEYRHSSEQLMVSLMRLGNLPPRFHGNVTLVEVRLLPDDRRPRQAKARGTGPDPEFSDCFVFQVKTNTCIEVPCVLMLCFCLRESSVRQAGSVCIYTKLGDVQISLCYSPSLQRLSVVFLRARGLQLLTGAGVCVQVSLQIHTQVVKSKRSCVLNGEANPYFNYRMSFKLRPQNLDEACLRFELQQPNSIRSEPPTLLGVLVLGPFMYARGPQLQHWMDMVNTPQEPVKLWHGLSRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBI6</accession>
    <name>A0A672YBI6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transient receptor potential cation channel, subfamily M, member 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YBI6"/>
    <dbReference type="Ensembl" id="ENSSORT00005000786.1">
        <property type="protein sequence ID" value="ENSSORP00005000760.1"/>
        <property type="gene ID" value="ENSSORG00005000476.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YBI6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1010">
        <property type="entry name" value="TRPM, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
        <property type="entry name" value="TRPM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032415">
        <property type="entry name" value="TRPM_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037162">
        <property type="entry name" value="TRPM_tetra_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF13">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY M MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25508">
        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16519">
        <property type="entry name" value="TRPM_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="568"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="8">
        <location>
            <begin position="364"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="TRPM tetramerisation" evidence="9">
        <location>
            <begin position="694"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="803" mass="93939" checksum="27A9DC2557310740" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YC24</accession>
    <name>A0A672YC24_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">med18</name>
        <name evidence="10" type="synonym">MED18</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 10">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030013964.1">
        <property type="nucleotide sequence ID" value="XM_030158104.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030013965.1">
        <property type="nucleotide sequence ID" value="XM_030158105.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030013967.1">
        <property type="nucleotide sequence ID" value="XM_030158107.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030013968.1">
        <property type="nucleotide sequence ID" value="XM_030158108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YC24"/>
    <dbReference type="FunCoup" id="A0A672YC24">
        <property type="interactions" value="1061"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002475.1">
        <property type="protein sequence ID" value="ENSSORP00005002414.1"/>
        <property type="gene ID" value="ENSSORG00005001436.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435588"/>
    <dbReference type="CTD" id="54797"/>
    <dbReference type="InParanoid" id="A0A672YC24"/>
    <dbReference type="OrthoDB" id="10018982at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 18"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0010">Activator</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="209" mass="23489" checksum="B812867721EAD676" modified="2020-06-17" version="1">MEAPPVTVMPVTGGTINMMEYLLQGSVLDQALESLLHRLRGLCDNMEPETFTDHELVYLLKGQQGNPFILRARRSLSHPTAPWHLRYLGQPEVGDKSRHALVRNCVDVAASHSLPEFLNEMGFRMDHEFVANGHVFRKGAMKVVVTKLSRILVPGNTENTERLSLSYLVELSVLAPAGQDTVSEDMRSFAEQLKPLVHLEKIDPSKQRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF58</accession>
    <name>A0A672YF58_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">znf536</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984082.1">
        <property type="nucleotide sequence ID" value="XM_030128222.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002375.1">
        <property type="protein sequence ID" value="ENSSORP00005002316.1"/>
        <property type="gene ID" value="ENSSORG00005001396.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414825"/>
    <dbReference type="CTD" id="9745"/>
    <dbReference type="InParanoid" id="A0A672YF58"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000075">
        <property type="entry name" value="Putative zinc finger protein 536"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000615">
        <property type="entry name" value="Zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000625">
        <property type="entry name" value="Zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051967">
        <property type="entry name" value="Krueppel_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16606">
        <property type="entry name" value="zf-C2H2_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="289"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="671"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="793"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="821"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="219"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="629"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="691"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="842"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="909"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1006"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1104"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1206"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1406"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="224"/>
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        <location>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002316.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1452" mass="157754" checksum="392049D611E935B7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFJ4</accession>
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        <recommendedName>
            <fullName evidence="1">GPALPP motifs-containing protein 1</fullName>
        </recommendedName>
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        <name evidence="4" type="primary">gpalpp1</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF12572"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002846.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="353" mass="39656" checksum="20D15D9BF2FE59B8" modified="2020-06-17" version="1">MSSNNIIGPALPPKHGVESDEDDDNEEGFAGPALPPGYKRGEPSSSSDESEQEVAVKRAKTSHTTAEWVHYFDSNICLLNRSKVEEDDGFFGPALPPGFKKQQSSPERPPVIGPALPPGFRRAADNDDDDEEDGEDFPGPALPPGYQAQPSSSEGEDEDVIGPMPAKGPVQDSVALDFERRALRMKEKLTGEETPEVLSRESWMTELPPELQHIGLGARTFKKRSGPENKDRSIWTDTPADRERKARERLEGKKKGETTKDSEPQVPRKDMEMAEKVSKYNESKRAESLMSLHSKKMKEKAKEKADTPVERRPFDRDADLQVNRFDEAQKQRLLKKSQELNTRFSHSKDRMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFR5</accession>
    <name>A0A672YFR5_9TELE</name>
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        <recommendedName>
            <fullName evidence="5">PTB domain-containing engulfment adapter protein 1</fullName>
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        <alternativeName>
            <fullName evidence="6">PTB domain adapter protein CED-6</fullName>
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        <name evidence="10" type="primary">LOC115413034</name>
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    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ced-6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YFR5"/>
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        <property type="interactions" value="474"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003298.1">
        <property type="protein sequence ID" value="ENSSORP00005003201.1"/>
        <property type="gene ID" value="ENSSORG00005001962.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFR5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01273">
        <property type="entry name" value="PTB_CED-6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GULP PTB domain containing engulfment adaptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF77">
        <property type="entry name" value="GULP PTB DOMAIN CONTAINING ENGULFMENT ADAPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="9">
        <location>
            <begin position="14"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="146"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060944"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00071263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="33247" checksum="626280F6D09F839B" modified="2020-06-17" version="1">MHTPEALAKHYIPYNAKFLGNTEVEAPKGTEVVEDAVRKLKFQRHIKKSEGQKVPKVELQISIYGVKILDPKTKDVHHNCQLHRISFCADDKTDKRIFTFICKDSESNKHLCYVFDSEKCAEEITLTIGQAFDLAYKKFLESGGKDVETRKQIGTLQRRIQELETENSELKKQLQDFEEQLMMVHGSPAGSVSIQTQSTDVFDMVPFSPVTPLVPTQANNASPPPPPTTLPPDISTSIYTVCKDLFGAEPFDPFTCASADFPPDIQSKLDEMQEGFKMGLTLEGTIFSLDPLDERC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGP9</accession>
    <name>A0A672YGP9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Microtubule-associated protein RP/EB family member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">mapre1b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YGP9"/>
    <dbReference type="Ensembl" id="ENSSORT00005003841.1">
        <property type="protein sequence ID" value="ENSSORP00005003732.1"/>
        <property type="gene ID" value="ENSSORG00005002246.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YGP9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000001">
        <property type="entry name" value="microtubule-associated protein RP/EB family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="14">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="15">
        <location>
            <begin position="177"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="129"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="32135" checksum="1AAEBA9DFA9B7EC9" modified="2020-06-17" version="1">MAVNVYSTSVTSDNLSRHDMLVWINESLQMNLTKIEMLCSGAVYCQFMDMLFPNSVPLRKVKFGAKLEHEYIHNFKLLQVAFKKMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKEYDPVEARQGQDAMPTPNPSTSALNKPPKKVLSQAPQRPPVAKVAPKMASPGVRKAGGGDEERAELLNELEMLKSTIQDMEKERDFYFGKLRNIELICQEKEGEGDPTLQRIIDILYATDVKMFTIPMFIFFSAQLHLSIIFSLYPTISTQKDKNTQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YK53</accession>
    <name>A0A672YK53_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0764 protein C16orf89 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">c8h16orf89</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YK53"/>
    <dbReference type="FunCoup" id="A0A672YK53">
        <property type="interactions" value="667"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005089.1">
        <property type="protein sequence ID" value="ENSSORP00005004949.1"/>
        <property type="gene ID" value="ENSSORG00005002969.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YK53"/>
    <dbReference type="OrthoDB" id="5949187at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031751">
        <property type="entry name" value="DUF4735"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33539">
        <property type="entry name" value="UPF0764 PROTEIN C16ORF89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33539:SF1">
        <property type="entry name" value="UPF0764 PROTEIN C16ORF89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15882">
        <property type="entry name" value="DUF4735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025578861" evidence="1">
        <location>
            <begin position="24"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="353" mass="40041" checksum="CAC41E149E68DF9D" modified="2020-06-17" version="1">MRTLGLQVAAVLALLAAVNGAEAEVIDDILNSLSRGAVFLERQHEHINLDGVVGFLMLQAELKEAIRTWPHTDPVSWAQRTTAVSLVKRLDQSFDKAVVALQQNDPKYYREFEPLLSWTFWLIPHGWSSTDPSLVYPSTMTTECYDEQLSDKCLTLLLGTWKMNGTPCIVTKPCRDTMTRFGCPHYSLSHQLLYFMIGKMKGCSNLLKGDTRPSRANMTERSYEKIFCSNMMKTNLDIIRDGLTEQTVDIFIENILICGLAGFSDFYKADWLHHILRLQDEELGCFGRDKSIISQIIGDELLEQLQPHRRVKRREKILPDGCSSHMTAVAVGALGGYLNYYLTEQDITKRPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLF8</accession>
    <name>A0A672YLF8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kelch-like ECH-associated protein 1a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">keap1a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting nfe2l2/nrf2 for ubiquitination. Keap1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of nfe2l2/nrf2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting nfe2l2/nrf2 nuclear accumulation and expression of phase II detoxifying enzymes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLF8"/>
    <dbReference type="Ensembl" id="ENSSORT00005005594.1">
        <property type="protein sequence ID" value="ENSSORP00005005436.1"/>
        <property type="gene ID" value="ENSSORG00005003230.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLF8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071379">
        <property type="term" value="P:cellular response to prostaglandin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071466">
        <property type="term" value="P:cellular response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030536">
        <property type="term" value="P:larval feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18458">
        <property type="entry name" value="BACK_KLHL19_KEAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000024">
        <property type="entry name" value="Kelch-like ECH-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047098">
        <property type="entry name" value="KEAP1_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF13">
        <property type="entry name" value="KELCH-LIKE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="55"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="575" mass="63266" checksum="B080B9FC674EEF2B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YN58</accession>
    <name>A0A672YN58_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily domain containing 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115423776</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YN58"/>
    <dbReference type="Ensembl" id="ENSSORT00005006296.1">
        <property type="protein sequence ID" value="ENSSORP00005006046.1"/>
        <property type="gene ID" value="ENSSORG00005003603.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YN58"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17407">
        <property type="entry name" value="MFS_MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF28">
        <property type="entry name" value="UNC93-LIKE PROTEIN MFSD11 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="454" mass="50251" checksum="E8E71A8055D211E8" modified="2020-06-17" version="1">MSPEGKKLLNIIILGFGFMFMFTAFQTCGNIEQTVLENFHSAEFNGTGYTSLAIIYMVAVIGPQLSMFFSGLLYSGYIAMFIYPYTWTFYTASVLVGIGAAVLWTAQGNVLAINSSDSTIGRNSGIFWALLQFSLFFGNLYVYCAWHGHVHITDKDRQTVFISLTVISLVGCFLFFLIRKPDAESASSEATEALLQGESDSNSVVSSPQPGLCSQALDAFSEFFRLCRHKPSCCLSFSLMVLYRLELTFYSGVYGTCIGAMTQFGQDAKSLIGISGICIGVGEILGEGVFGMLNKCNRFGRNPVVLFGLVIHFVAFYLIFLNIASDAPKQETDARAFMQPNLGVALLCSFLLGLGDSCFNTQLLSIIGFMFKDDSAPAFAVFKFIQSIMAAVAFSYSNFLLLHWQLLILVLGGFLGTMTFFVAEWAAESRRRVGLRQHLIGYYNWIYDQKWADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPS7</accession>
    <name>A0A672YPS7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Semaphorin-3D-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">si:dkey-49n23.1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPS7"/>
    <dbReference type="FunCoup" id="A0A672YPS7">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006854.1">
        <property type="protein sequence ID" value="ENSSORP00005006594.1"/>
        <property type="gene ID" value="ENSSORG00005003867.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPS7"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF69">
        <property type="entry name" value="SEMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025449685" evidence="12">
        <location>
            <begin position="42"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="14">
        <location>
            <begin position="56"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="594"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="801"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="820" mass="92610" checksum="016E0941FA17180D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQC9</accession>
    <name>A0A672YQC9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin-protein ligase ZNRF3</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RING-type E3 ubiquitin transferase ZNRF3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Zinc/RING finger protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">znrf3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZNRF3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_029999616.1">
        <property type="nucleotide sequence ID" value="XM_030143756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YQC9"/>
    <dbReference type="FunCoup" id="A0A672YQC9">
        <property type="interactions" value="922"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007063.1">
        <property type="protein sequence ID" value="ENSSORP00005006801.1"/>
        <property type="gene ID" value="ENSSORG00005003953.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425900"/>
    <dbReference type="CTD" id="84133"/>
    <dbReference type="InParanoid" id="A0A672YQC9"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16799">
        <property type="entry name" value="RING-H2_ZNRF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000018">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZNRF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000075">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase rnf43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040700">
        <property type="entry name" value="ZNRF-3_ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051073">
        <property type="entry name" value="ZNRF3_Arkadia_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045903">
        <property type="entry name" value="ZNRF3_Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16200">
        <property type="entry name" value="RING ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18212">
        <property type="entry name" value="ZNRF_3_ecto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="269"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="600"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="650"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="879"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="608"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="879"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="898" mass="97177" checksum="F50618C1811549BA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQN4</accession>
    <name>A0A672YQN4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mohawk homeobox b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YQN4"/>
    <dbReference type="Ensembl" id="ENSSORT00005007166.1">
        <property type="protein sequence ID" value="ENSSORP00005006904.1"/>
        <property type="gene ID" value="ENSSORG00005004000.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YQN4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN MOHAWK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="60"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="62"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="305" mass="34314" checksum="F3C115B2A08BEA6B" modified="2020-06-17" version="1">MNKVAVMKSDTLLRLEDNGRVEERSRLNCVEIPQTSLTDGQSTDLLVFSDCYSSRSRLGGVKVRHKRQVLQDMARPLKHWLYKHRDNPYPTKTEKVLLALGSHMTLVQVSNWFANARRRLKNTVRQPDLSWALRIKLYNKYIQGNAERLSVCSDDTDSDDECPLQNPISQSDFGRSSSHKSVLQKQGSVLAMADSANSDDSTSPPSKYKSSLLNRYLNDTLRHMMAAKADGVTAARKRRSHSESFSSNECERDVVSPASSYETEANFVYHMECAICSASASCHGGNVQSSLKDNYRMSETLFFPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSR5</accession>
    <name>A0A672YSR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115428710</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YSR5"/>
    <dbReference type="Ensembl" id="ENSSORT00005007826.1">
        <property type="protein sequence ID" value="ENSSORP00005007546.1"/>
        <property type="gene ID" value="ENSSORG00005004298.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007827.1">
        <property type="protein sequence ID" value="ENSSORP00005007547.1"/>
        <property type="gene ID" value="ENSSORG00005004298.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000005">
        <property type="entry name" value="MHC class I antigen ZKA transcript variant 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF193">
        <property type="entry name" value="LOC571647 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627670" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="216"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="427"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="364"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="448"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="474" mass="53267" checksum="07CCE6BD0C3BE45F" modified="2020-06-17" version="1">MSKMYAIAFLLVLATGVTVNSETPSDRHSLTYIYTALSKDPKIPGIHEFTAMGILDSRVIDYFDSTTQVKTPKTHWMRERLEPEYWEKGTRSRKSKQQWFKVNLDILKERMNQTDNDIHVLQWRHGCEGVKKGNGSLEYSHGLDMYSYDGNDFLSFDDSSSVWVAPVKAAEQTKRKWDEVQVLKDYTKGYLEKECMDWLRKFLTYGEKDMYAAKPPEVYMFANNAKSKTNVVLNCMATGFYPPEIQIYIKRNSRILTKDDDVMSTGTRPNEDNTFQRRDSMEILRSDVAMYSCHVVHHPTGVNIIKVWDRKLPDPDDEPDSPVPVGVIEGVLGVVAVLGLGLVVLGILYKKGKLDRVIHRNPQQLPPQNPRPAVNPVPHPAPVAVEVEPAAQDPLIQQNEPKKGKNYNCGTFSQIPHEPLHTVNTNPPMFPFITDGSQGSLESKDSGHSSNDATSSGASNRGSGDGSHESLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0G2</accession>
    <name>A0A672Z0G2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z0G2"/>
    <dbReference type="Ensembl" id="ENSSORT00005010931.1">
        <property type="protein sequence ID" value="ENSSORP00005010591.1"/>
        <property type="gene ID" value="ENSSORG00005005726.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0G2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018061">
        <property type="entry name" value="Retropepsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051320">
        <property type="entry name" value="Viral_Replic_Matur_Polypro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064">
        <property type="entry name" value="POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064:SF37">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00077">
        <property type="entry name" value="RVP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Peptidase A2" evidence="7">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="225"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="37698" checksum="A5446814912AB3E4" modified="2020-06-17" version="1">LTVNGVNLPFLVDSGATYSVIKHADLTPTPQLSGKTIVSMLAGGKLDRESFTVPLTCVSQECSFQHSFLLSPIYPINLCGRDLMCKLGVKLESGPEGLMITQGNTVTTYTIQPGAHVSPPDDLYCASHFSPGGPDSTYEEKPCQKQYPLKQEAIDGITPVFEAYLNAGIIVPCPDSPVRTPIFPLKKARTPPVPDTWRFIQDLQAVNSRGATCAHGSKSTHNFVTNSAPILVHVAFRNKMYTWTCIPQGYCEAPTIYNSILADSLSHLDLPPGVAPLQYVDDLLLYSPAVAKALVTDILFCFHWGIPKKISSDNGSRFVNTALTELGNMLGFDLKTHCVYHPQSGGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z659</accession>
    <name>A0A672Z659_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nicotinamide riboside kinase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mibp2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>beta-D-ribosylnicotinate + ATP = nicotinate beta-D-ribonucleotide + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25568"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.173"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>beta-nicotinamide D-riboside + ATP = beta-nicotinamide D-ribonucleotide + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14017"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15927"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the uridine kinase family. NRK subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z659"/>
    <dbReference type="FunCoup" id="A0A672Z659">
        <property type="interactions" value="225"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012696.1">
        <property type="protein sequence ID" value="ENSSORP00005012299.1"/>
        <property type="gene ID" value="ENSSORG00005006492.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z659"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061769">
        <property type="term" value="F:nicotinate riboside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050262">
        <property type="term" value="F:ribosylnicotinamide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019363">
        <property type="term" value="P:pyridine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02024">
        <property type="entry name" value="NRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000853">
        <property type="entry name" value="Nicotinamide riboside kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="206" mass="23601" checksum="EB5F74D15780C61C" modified="2020-06-17" version="1">NMKYIIGIGGVTNGGKTTLTNRLIKNLPNCCVVHQDDFFKPQDQIEVDEDGFKQYDVITALDMDAMMSTIYAWLENPVKFEKSHGVNNTLDNEILPQSDQKEEETHILIVEGFLLYTYGPLIDVLNQRYFISIPYEECKKRRCSRNYAVPDPPGLFDGHVWPMYLKHKNIMEGSGVDVVQLDGTKSKEQLFNFVYGNILNNIQKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDN7</accession>
    <name>A0A672ZDN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Lissencephaly-1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PAFAH1B1</name>
        <name evidence="15" type="synonym">LIS1</name>
        <name evidence="18" type="synonym">LOC115431588</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. May be required for proliferation of neuronal precursors and neuronal migration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Can self-associate. Component of the cytosolic PAF-AH (I) heterotetrameric enzyme, which is composed of PAFAH1B1 (beta), PAFAH1B2 (alpha2) and PAFAH1B3 (alpha1) subunits. The catalytic activity of the enzyme resides in the alpha1 (PAFAH1B3) and alpha2 (PAFAH1B2) subunits, whereas the beta subunit (PAFAH1B1) has regulatory activity. Trimer formation is not essential for the catalytic activity. Interacts with dynein, dynactin, nde1 and ndel1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Can self-associate. Interacts with dynein, dynactin, NDE1 and NDEL1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
            <location evidence="15">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
            <location evidence="15">Cytoskeleton</location>
            <location evidence="15">Microtubule organizing center</location>
            <location evidence="15">Centrosome</location>
        </subcellularLocation>
        <text evidence="15">Localizes to the plus end of microtubules and to the centrosome.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Dimerization mediated by the LisH domain may be required to activate dynein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDN7"/>
    <dbReference type="Ensembl" id="ENSSORT00005015383.1">
        <property type="protein sequence ID" value="ENSSORP00005014949.1"/>
        <property type="gene ID" value="ENSSORG00005007601.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDN7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048854">
        <property type="term" value="P:brain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000038">
        <property type="entry name" value="Lissencephaly-1 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000002">
        <property type="entry name" value="Platelet-activating factor acetylhydrolase ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056795">
        <property type="entry name" value="PAC1-like_LisH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24951">
        <property type="entry name" value="LisH_PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037647">
        <property type="entry name" value="Dynein_regulator_Lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 15" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2 15" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 15" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 15" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10 15" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 15" id="KW-0813">Transport</keyword>
    <keyword evidence="4 16" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PAC1-like LisH-like dimerisation" evidence="17">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="252"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="356"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="398"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="432" mass="49214" checksum="DB232468D688FCED" modified="2020-06-17" version="1">MLRGVGLFHTCYHPHHLSVFAYIHLLWLSERFCVFLKTSAWGQDELTIADYLRSNGYEEAYSTFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEITLGGPVTQKRDPKEWIPRPPEKYALSGHLFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDQTGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTMKMWEVATGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESAYPTILDATGSETKKSGKPGPFLLSGSRDKTIKMWDVSIGMCLMTLAGHDNWVRGILFHPGGKFIVTCADDKTLRIWDYKNKRCMKTLSAHEHFVTSLDFHKAAPYVVTGSVDQTVKVWECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFG1</accession>
    <name>A0A672ZFG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">dnmt3ba</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="Ensembl" id="ENSSORT00005016056.1">
        <property type="protein sequence ID" value="ENSSORP00005015564.1"/>
        <property type="gene ID" value="ENSSORG00005004249.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZFG1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051718">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000008">
        <property type="entry name" value="DNA (Cytosine-5)-methyltransferase 3A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.50">
        <property type="entry name" value="PWWP, helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025766">
        <property type="entry name" value="ADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018117">
        <property type="entry name" value="C5_DNA_meth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040552">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049554">
        <property type="entry name" value="DNMT3_ADD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068:SF53">
        <property type="entry name" value="DNA (CYTOSINE-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068">
        <property type="entry name" value="DNA CYTOSINE-5- -METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17980">
        <property type="entry name" value="ADD_DNMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21255">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51533">
        <property type="entry name" value="ADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00094">
        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="14">
        <location>
            <begin position="15"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="15">
        <location>
            <begin position="827"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="994"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="792"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="1222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1424" mass="160487" checksum="C032BB5824BC2BB2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGB1</accession>
    <name>A0A672ZGB1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Guanylyl cyclase-activating protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">guca1aa</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZGB1"/>
    <dbReference type="Ensembl" id="ENSSORT00005016577.1">
        <property type="protein sequence ID" value="ENSSORP00005016075.1"/>
        <property type="gene ID" value="ENSSORG00005008127.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGB1"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000052">
        <property type="entry name" value="Guanylate cyclase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF164">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="130"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="189" mass="21817" checksum="AC0B139932481AFA" modified="2020-06-17" version="1">MGNTSGSTVDDLQAVEMHLWYKKFMTECPSGQLTLHEFKQFFGLRGLDPEANAYIEQMFRTFDMNKDGTIDFMEYVAALSLVMRGKMEHKLRWYFKLYDVDGNGCIDRHELLNIIKAIRAINGNESQETSAEEFTNRVFDRIDINGDGELSLEEFVAGARSDEEFMDVMMKSLDLAHIVAMIHNRRHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGX1</accession>
    <name>A0A672ZGX1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apolipoprotein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">apodb</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZGX1"/>
    <dbReference type="FunCoup" id="A0A672ZGX1">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016818.1">
        <property type="protein sequence ID" value="ENSSORP00005016310.1"/>
        <property type="gene ID" value="ENSSORG00005008272.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGX1"/>
    <dbReference type="OrthoDB" id="565904at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19437">
        <property type="entry name" value="lipocalin_apoD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000003">
        <property type="entry name" value="Apolipoprotein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026222">
        <property type="entry name" value="ApoD_vertbrte"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002969">
        <property type="entry name" value="ApolipopD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022271">
        <property type="entry name" value="Lipocalin_ApoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF15">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036893">
        <property type="entry name" value="Lipocalin_ApoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02058">
        <property type="entry name" value="APODVERTBRTE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01219">
        <property type="entry name" value="APOLIPOPROTD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025720401" description="Apolipoprotein D" evidence="11">
        <location>
            <begin position="17"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="12">
        <location>
            <begin position="33"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="183" mass="20842" checksum="A3DB419E28EE94D6" modified="2020-06-17" version="1">MSATAVLLLLLPLVSAQTYHWGSCPTPKVQPGFDLEKYLGRWYEIWKLPASFERGKCIEANYALRKDGTIQVLNSQFYKDKTRKVEGTAIVPDARDAARLGVSFSYFTPYSPYWVLSTDYTSVAVVYSCTDILRIFHVEYAWILGRSRFLPRETVVYAQDLLVEEGIDLTRMTATDQTGCKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHA4</accession>
    <name>A0A672ZHA4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial</fullName>
            <ecNumber evidence="3">6.4.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-methylcrotonyl-CoA carboxylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mccc2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-methylbut-2-enoyl-CoA + hydrogencarbonate + ATP = 3-methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13589"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Probably a dodecamer composed of six biotin-containing alpha subunits (MCCC1) and six beta (MCCC2) subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.4" evidence="3"/>
    <dbReference type="RefSeq" id="XP_029998431.1">
        <property type="nucleotide sequence ID" value="XM_030142571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZHA4"/>
    <dbReference type="Ensembl" id="ENSSORT00005016339.1">
        <property type="protein sequence ID" value="ENSSORP00005015843.1"/>
        <property type="gene ID" value="ENSSORG00005007596.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425188"/>
    <dbReference type="CTD" id="64087"/>
    <dbReference type="InParanoid" id="A0A672ZHA4"/>
    <dbReference type="OrthoDB" id="439921at2759"/>
    <dbReference type="UniPathway" id="UPA00363">
        <property type="reaction ID" value="UER00861"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905202">
        <property type="term" value="C:methylcrotonoyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000004">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000007">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855:SF13">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="11">
        <location>
            <begin position="50"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="12">
        <location>
            <begin position="310"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="564" mass="61780" checksum="C84674DD7A596550" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHW6</accession>
    <name>A0A672ZHW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Origin recognition complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">orc5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004989.1">
        <property type="nucleotide sequence ID" value="XM_030149129.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030004990.1">
        <property type="nucleotide sequence ID" value="XM_030149130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZHW6"/>
    <dbReference type="FunCoup" id="A0A672ZHW6">
        <property type="interactions" value="1720"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016574.1">
        <property type="protein sequence ID" value="ENSSORP00005016072.1"/>
        <property type="gene ID" value="ENSSORG00005008125.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429566"/>
    <dbReference type="CTD" id="5001"/>
    <dbReference type="InParanoid" id="A0A672ZHW6"/>
    <dbReference type="OrthoDB" id="365981at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000673">
        <property type="entry name" value="Origin recognition complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048866">
        <property type="entry name" value="ORC5_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21639">
        <property type="entry name" value="ORC5_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="ORC5 lid" evidence="12">
        <location>
            <begin position="213"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="11">
        <location>
            <begin position="312"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="445" mass="51155" checksum="76A6B1B2EEC12894" modified="2020-06-17" version="1">MIARLQQTGYEEDRLQRVAKLLPCRELQAGTLLSIMGQPQQYSYPSVFIYGHRASGKSHVMHILLKELELPHATVSCVECVSVALLFEQVLLSFFGCDAASLLPRSPSLSDFVRIYRQQCLEMPAKQTRYVVMEKAELLRDMDANLLPAILRLQELVEDNVTVIFLSEIVWDKFRPNTGCFEPLLLHFPDYSKGELQQILSQDKHPSYSAEFYSSYINILLGVFYSVCRDLRELRHLAALNFSKFCEPLAEGKVKESDTHKLWRNIEPHLKKAMQTVYLREVSSLQWEQMQQVEEKETGPLRGLSAHTHVELPYYSKFLLIAAYLASYNPARTDKRFFLKHHGKIKKTNFLKKNEKTSNHLLGPKPFPLDRLLAIFYSVVDSRVAPTASIFSQISSLVTLQLLTQVSHDDQLDAPKYKCAVSMDFICAISRTVNFDIVKYLYDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZL60</accession>
    <name>A0A672ZL60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZL60"/>
    <dbReference type="Ensembl" id="ENSSORT00005017712.1">
        <property type="protein sequence ID" value="ENSSORP00005017197.1"/>
        <property type="gene ID" value="ENSSORG00005008621.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZL60"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF295">
        <property type="entry name" value="AZ2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="38"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="440" mass="50603" checksum="77FC496F73DAB8B9" modified="2020-06-17" version="1">MELRNHKKTHVPKILPCPFCQEKCANYEQFLDHIKTHGITKATRPTRPERNEEAKTQETMELGNNDNHESDDGSEEGNIDADSENNEVQTEESELKMENKDSEAKSNGHKKDKALKSKKKGHFCPICVGRRFRGPNKLARHMRSHTKEKPFSCPVCALTFSQSYHMTRHLRNQHGLGHYICTKCGKNLETWQELNVHKKTHAADDLSCPVCDKQFKDKASFTSHIKSHKTIPSSPQCLICAECGKVFGRMYHLKRHISKCPHCDAVFLKLTALKYHLRTHTEERPYQCTCCIETFEEKGDLEQHRLKHRKFKKERPYSCTRCDNAFLTLTDIHTGERPHLCSICGNGFPSAASLKLHVHIHTGEKPYQCSQCTKSFRSSSGLRLHSRQHMEVRPSYECPQCGRSYGRMTELKMHQRYHTGDKPYSDVEHLLAICGPSHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZPZ0</accession>
    <name>A0A672ZPZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Cadherin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">cdh1</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 22">Cell membrane</location>
            <topology evidence="2 22">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
            <location evidence="5">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZPZ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005019819.1">
        <property type="protein sequence ID" value="ENSSORP00005019261.1"/>
        <property type="gene ID" value="ENSSORG00005008892.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZPZ0"/>
    <dbReference type="OrthoDB" id="6079678at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007398">
        <property type="term" value="P:ectoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001841">
        <property type="term" value="P:neural tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000191">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000022">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000027">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000031">
        <property type="entry name" value="Cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014868">
        <property type="entry name" value="Cadherin_pro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF319">
        <property type="entry name" value="CADHERIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08758">
        <property type="entry name" value="Cadherin_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01055">
        <property type="entry name" value="Cadherin_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 21 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 22" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="15" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="18 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 26" id="KW-0732">Signal</keyword>
    <keyword evidence="8 22 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025452154" description="Cadherin-1" evidence="26">
        <location>
            <begin position="25"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="789"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="27">
        <location>
            <begin position="242"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="27">
        <location>
            <begin position="349"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="27">
        <location>
            <begin position="461"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="27">
        <location>
            <begin position="573"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="27">
        <location>
            <begin position="697"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="121"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="966" mass="107598" checksum="4ECDEB2F56C0A7AA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQQ4</accession>
    <name>A0A672ZQQ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Acylglycerol kinase, mitochondrial</fullName>
            <ecNumber evidence="5">2.7.1.107</ecNumber>
            <ecNumber evidence="22">2.7.1.138</ecNumber>
            <ecNumber evidence="23">2.7.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Multiple substrate lipid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">agk</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:34071"/>
            <dbReference type="ChEBI" id="CHEBI:74938"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:43329"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-(9Z-octadecenoyl)-sn-glycerol + ATP = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41079"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:75757"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41080"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1-hexadecanoyl-sn-glycerol + ATP = 1-hexadecanoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:75542"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:43309"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + ATP = 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43316"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
            <dbReference type="ChEBI" id="CHEBI:78209"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:43317"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>N-(hexanoyl)sphing-4-enine + ATP = N-hexanoylsphing-4-enine 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43312"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63867"/>
            <dbReference type="ChEBI" id="CHEBI:82959"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:43313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1-acyl-sn-glycerol + ATP = a 1-acyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33747"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:64683"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33748"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 2-acylglycerol + ATP = a 2-acyl-sn-glycerol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39847"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:64982"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:39848"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a monoacylglycerol + ATP = a monoacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19293"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77589"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.94"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:19294"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>an N-acylsphing-4-enine + ATP = an N-acylsphing-4-enine 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17929"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57674"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.138"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:17930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the AGK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="5"/>
    <dbReference type="EC" id="2.7.1.138" evidence="22"/>
    <dbReference type="EC" id="2.7.1.94" evidence="23"/>
    <dbReference type="RefSeq" id="XP_029993318.1">
        <property type="nucleotide sequence ID" value="XM_030137458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZQQ4"/>
    <dbReference type="FunCoup" id="A0A672ZQQ4">
        <property type="interactions" value="704"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020149.1">
        <property type="protein sequence ID" value="ENSSORP00005019590.1"/>
        <property type="gene ID" value="ENSSORG00005009610.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421532"/>
    <dbReference type="CTD" id="55750"/>
    <dbReference type="InParanoid" id="A0A672ZQQ4"/>
    <dbReference type="OrthoDB" id="9979394at2759"/>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047620">
        <property type="term" value="F:acylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001729">
        <property type="term" value="F:ceramide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045579">
        <property type="entry name" value="AGK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF31">
        <property type="entry name" value="ACYLGLYCEROL KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19712">
        <property type="entry name" value="AGK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="30">
        <location>
            <begin position="61"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00025749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00026096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00026098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00026142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00030553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00044480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="423" mass="48051" checksum="D8D7D746A98A6066" modified="2020-06-17" version="1">MARVVKVFRTLRNHWKKSTFAVCVLSYGGHWLYGKYCDSVLRREACLEAREFGRQLIGSQERLRKATVILNPAACGGKANNLFEKNAAPILHLAGVEITIVKTDYEGQAKKLIDLMEQTDMLIVAGGDGTLQEVITGLLRRPDQDIFSNTPIGFIPLGSHNSLSPSLHLLSDNKVKNITSATLSILKGETVPLDVLQIKGEKEQPVFALMGLRWGAFRDVAATISKYWYLGPLKTNAAHWFRTLQEWPLVRDISISYLPPSLRPPDAPPQKPPRPNLLYRIARRWKNYWNPPVEEPPKVEEPEKWEEQKLSTFELFVQTHNKNPVKRRVEDSLMICAEPDDFSVSEFITVGNKKEEDPTVFTKSSTKLEVGACRLQLPEGAGGFYNIDNEEYEAMPVEVRLLPRKLHFFISAERRDQLLSQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZRS2</accession>
    <name>A0A672ZRS2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZRS2"/>
    <dbReference type="Ensembl" id="ENSSORT00005020438.1">
        <property type="protein sequence ID" value="ENSSORP00005019876.1"/>
        <property type="gene ID" value="ENSSORG00005009707.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZRS2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015631">
        <property type="entry name" value="CD2/SLAM_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080:SF59">
        <property type="entry name" value="HEPATIC AND GLIAL CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025607866" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="124"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="306" mass="33997" checksum="C58CC1B573262708" modified="2020-06-17" version="1">MACFVPSLGFIVLLGFVTTSAAYRDTCDLYATVGQNLTLPFVYERLESRDNLQWIHNNKFIFKRHQGRVTFGKPDDVSVTGSLLLKTLKSSSAGLYQADVSYPNGTLAKTWTGYLCLMDKVTKPYLSFVCDYKSGVVTLNCHVAKPQDVMFSWTLDERPFSETRSTGSITLSLFKEWGGFTCAAANKVSKEKSDIVRPMCKKPSPSPPPLLCFTSKAVVAVLAGGVSVILLLLFIIIITLCCCYRRNKTTKSKGTGQLRMLSLTPRSADPVSSDYETMHASEPSPLPRYWNWSCLCVTNVIHNLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUL4</accession>
    <name>A0A672ZUL4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kelch domain containing 8A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">klhdc8a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZUL4"/>
    <dbReference type="FunCoup" id="A0A672ZUL4">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021231.1">
        <property type="protein sequence ID" value="ENSSORP00005020654.1"/>
        <property type="gene ID" value="ENSSORG00005010017.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZUL4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="346" mass="37961" checksum="358F6BB3DC6D6133" modified="2020-06-17" version="1">MAVPSAQEFHWQSLAKLSSGRVYHTLTEVGGQMYMLGGCDAAGRPCATLELYSPEGDRWISLAPMPTPRAGAAVAVLGKQILVVGGVGEDQSPLKVVELYSTEEGRWRKRSALREALMGLSITVKDGRALAVGGMGADLLPRSILQQYDLRKDVWALLPPMPTPRYDANTHLLGNKLYVAGGRQCKRPVKAFEVYDSETRTWTTLPMMPCKRSYGGVIWDPAGRLCLLGGLRQGGGHQSSKFTKNINIFDTNQGVWLKSEDTVAMKTKRADFAAAFLRGRMIVAGGLGHEPTALDTVDAFHPERKKWERLAPMTFPRCSASSIVIRDRLLVVGGVNQEVELFCFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZV09</accession>
    <name>A0A672ZV09_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZV09"/>
    <dbReference type="Ensembl" id="ENSSORT00005021753.1">
        <property type="protein sequence ID" value="ENSSORP00005021125.1"/>
        <property type="gene ID" value="ENSSORG00005010305.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZV09"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000017">
        <property type="entry name" value="papilin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF375">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="2">
        <location>
            <begin position="9"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="75" mass="8648" checksum="B438EF203900346B" modified="2020-06-17" version="1">INHGGTERCLEPMSEGPCSEYILLWYFHQHSGKCRPFVYGGCGGNRNRFSSRQECQIWCGMESRGKQIQEIYGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWM9</accession>
    <name>A0A672ZWM9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized LOC115423579</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115423579</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029996317.1">
        <property type="nucleotide sequence ID" value="XM_030140457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZWM9"/>
    <dbReference type="Ensembl" id="ENSSORT00005022142.1">
        <property type="protein sequence ID" value="ENSSORP00005021496.1"/>
        <property type="gene ID" value="ENSSORG00005010524.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423579"/>
    <dbReference type="InParanoid" id="A0A672ZWM9"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF170">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="37"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="265"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="265"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="668" mass="69344" checksum="D9C7A02DC8D375D8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXK3</accession>
    <name>A0A672ZXK3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-binding protein 5</fullName>
            <shortName evidence="4">SH3BP-5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sh3bp5la</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Functions as guanine nucleotide exchange factor (GEF) for RAB11A.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with GDP-bound and nucleotide-free forms of RAB11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Colocalizes with RAB11A on cytoplasmic vesicle membranes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal half of the protein mediates interaction with RAB11A and functions as guanine nucleotide exchange factor. Four long alpha-helices (interrupted by a central kink) assemble into coiled coils, giving rise to a 'V' shape.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SH3BP5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZXK3"/>
    <dbReference type="Ensembl" id="ENSSORT00005022655.1">
        <property type="protein sequence ID" value="ENSSORP00005022000.1"/>
        <property type="gene ID" value="ENSSORG00005010743.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZXK3"/>
    <dbReference type="OrthoDB" id="446789at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007940">
        <property type="entry name" value="SH3BP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423:SF8">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05276">
        <property type="entry name" value="SH3BP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="389"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="192"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="437" mass="49235" checksum="3832CF9FE6E81FCC" modified="2020-06-17" version="1">MEPEDLRESPAGSGESDTGDWRDVIPDGDAEVKAAETNETTAETTLRDTCEEEGEIKKPLSPEEHLHSPYEEELDPRIQEELEHLNEASAEINKLELQLDDARSGYRKILTESARKLNAQSSQLGGCIEKARPYYEARRLAKEAQQETQKAALSYERAVSMHTAAREMVYVAEQGLMADGKNTLDPTWQEMLNHATSKVNEAEEERLRSEREHMRVTHACQEAEARVQMLQKSLKRVILKSKPYFELKAQFNHILEEHKSKVLQLEQHVAKVKTRYSIALRNLEQISEQIHAQRGQDQAEGGRLSICVGRSPPVGAESDIRVQEEGGACGGGSMMKDRVDAAIDLVEKYKEKESEKERERAGSDSLSVFSLQTIASDLEKYDSIEHLGDLSDVGSVTGDDGEKEKGGVKEKRDKLTETTAKERQQQFYKAHHRSFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXW4</accession>
    <name>A0A672ZXW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="3 7">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115423931</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="3 7"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZXW4"/>
    <dbReference type="Ensembl" id="ENSSORT00005022569.1">
        <property type="protein sequence ID" value="ENSSORP00005021916.1"/>
        <property type="gene ID" value="ENSSORG00005010697.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZXW4"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000021">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00158">
        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="410" mass="44806" checksum="B57576487B7BD5B9" modified="2020-06-17" version="1">MTHAYPFLSTEQKKELSDIAQRIVAAGKGILAADESTGSMAKRFQGINVENTEENRRLYRQLLFTADDRVNPCIGGVIFFHETLYQKTDDGKLFPQLIKEKGIVVGIKVDKGVVPLAGTNGETTTQGLDGLYERCAQYKKDGADFAKWRCVLKITPTTPSNLAILENANVLARYASICQMVTTAPSASLLRTQATTKRRCVFCCHDTDYCVLCHLLLNAFAFVPPQHGIVPIVEPEILPDGDHDLQRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTAGHSCPTKYSPQEIALATVTALRRAVPPAVPGVTFLSGGQSEEEATVNLNAINQCPLHRPWALTFSFGRALQASALKAWGGKKENGKACQDEYIKRALNNSMAAVGKYVSSENKGAAAQESLFVADHAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXX3</accession>
    <name>A0A672ZXX3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IQ motif and Sec7 domain ArfGEF 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRAG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZXX3"/>
    <dbReference type="Ensembl" id="ENSSORT00005022766.1">
        <property type="protein sequence ID" value="ENSSORP00005022110.1"/>
        <property type="gene ID" value="ENSSORG00005010794.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZXX3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
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        <property type="entry name" value="PH_IQSEC"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.30.29.30:FF:000004">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR033742">
        <property type="entry name" value="IQSEC_PH"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF314">
        <property type="entry name" value="IQ MOTIF AND SEC7 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16453">
        <property type="entry name" value="IQ_SEC7_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="406"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="209"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="353"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="872" mass="97501" checksum="59C6227D7B086898" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0N1</accession>
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        <submittedName>
            <fullName evidence="15">EBF transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115433508</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="11">Forms either a homodimer or a heterodimer with a related family member.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the COE family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005022789.1"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="COE_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01175">
        <property type="entry name" value="IPT_COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4280:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3180:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001696">
        <property type="entry name" value="Transcription factor COE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3180">
        <property type="entry name" value="Transcription factor COE1, DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="COE_DBD"/>
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    <dbReference type="InterPro" id="IPR038173">
        <property type="entry name" value="COE_DBD_sf"/>
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        <property type="entry name" value="COE_HLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038006">
        <property type="entry name" value="COE_IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003523">
        <property type="entry name" value="Transcription_factor_COE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018350">
        <property type="entry name" value="Transcription_factor_COE_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10747">
        <property type="entry name" value="TRANSCRIPTION FACTOR COE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16422">
        <property type="entry name" value="COE1_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16423">
        <property type="entry name" value="COE1_HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01345">
        <property type="entry name" value="COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="IPT/TIG" evidence="14">
        <location>
            <begin position="260"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="447"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="465"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="651" mass="71613" checksum="79D7F0317E92AC62" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3M5</accession>
    <name>A0A673A3M5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADH dehydrogenase (ubiquinone) complex I, assembly factor 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ndufaf6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I) at early stages. May play a role in the biogenesis of complex I subunit MT-ND1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NDUFAF6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030015120.1">
        <property type="nucleotide sequence ID" value="XM_030159260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A3M5"/>
    <dbReference type="Ensembl" id="ENSSORT00005023902.1">
        <property type="protein sequence ID" value="ENSSORP00005023228.1"/>
        <property type="gene ID" value="ENSSORG00005011231.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436397"/>
    <dbReference type="CTD" id="137682"/>
    <dbReference type="InParanoid" id="A0A673A3M5"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000013">
        <property type="entry name" value="NADH dehydrogenase (ubiquinone) complex I, assembly factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181:SF13">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE) COMPLEX I, ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="331" mass="37912" checksum="D1FE39429F6B5BA5" modified="2020-06-17" version="1">MAAGISAKHGVLCSKTSLYYGLHRRMSPSTGCIQRAAELAAFRAASSASLDSQQNEKYCLELVRSRDYDGFVCSLLLPEEARRSALALRAFNVELAQVKDSVSQKTIGLMRMQFWKTAIDDIYRDEPPNQPVSAELWRAVRKHYLTKRWLQRIITEREKDLDDRAYRNLQELEAYSENTQSSLLYLLVECLGLKDVHADHAASHIGKAQGIVTCLRATPYHSSRRKVYLPMDICMLHGASQEDFIRGSREQNIRDVVYDIASQAHVHLQHARSFSHNVPAAATPAFLQTVVLEDYLQRVRRADFDVFHPSLQNRNPLLPFQLYLRSWKKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A657</accession>
    <name>A0A673A657_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 3-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">p3h1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the leprecan family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.7" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673A657"/>
    <dbReference type="FunCoup" id="A0A673A657">
        <property type="interactions" value="593"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024854.1">
        <property type="protein sequence ID" value="ENSSORP00005024143.1"/>
        <property type="gene ID" value="ENSSORG00005010128.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A657"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019797">
        <property type="term" value="F:procollagen-proline 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032963">
        <property type="term" value="P:collagen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000003">
        <property type="entry name" value="Prolyl 3-hydroxylase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056585">
        <property type="entry name" value="Leprecan_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039575">
        <property type="entry name" value="P3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14049">
        <property type="entry name" value="LEPRECAN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14049:SF5">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23557">
        <property type="entry name" value="TPR_leprecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="10" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="16">
        <location>
            <begin position="509"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="652"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="657"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="677"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="719"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="742"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="802"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="821" mass="92686" checksum="5E016F7B8DC0BC72" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6T4</accession>
    <name>A0A673A6T4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translocating chain-associated membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tram1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979672.1">
        <property type="nucleotide sequence ID" value="XM_030123812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A6T4"/>
    <dbReference type="FunCoup" id="A0A673A6T4">
        <property type="interactions" value="859"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025081.1">
        <property type="protein sequence ID" value="ENSSORP00005024368.1"/>
        <property type="gene ID" value="ENSSORG00005011729.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411616"/>
    <dbReference type="CTD" id="23471"/>
    <dbReference type="InParanoid" id="A0A673A6T4"/>
    <dbReference type="OrthoDB" id="3053196at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016447">
        <property type="entry name" value="Translocation_assoc_membrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12371:SF3">
        <property type="entry name" value="TRANSLOCATING CHAIN-ASSOCIATED MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12371">
        <property type="entry name" value="TRANSLOCATION ASSOCIATED MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005449">
        <property type="entry name" value="Translocation_assoc_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="14">
        <location>
            <begin position="117"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="331"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="332"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="370" mass="42451" checksum="7805EA96B69C2E19" modified="2020-06-17" version="1">MGIRKKTNKNPPVLSHEFVIQNHADIVSCVAMVFLLGLMFEVTSKFAVLFITVQYNVTISTNEGPEETAVNHFHHGIKDLATIFFYMLVAIIMHAIIQEYVLDKINRKKHFSKTKHSKFNESGQLSAFYLFSFGWGASILLSENFLSNPVSLWEGYPHTLMPFQMKFYYICQLGYWLHALPELYFQKTKKEDIPRQLVYIFLYLVHIAGAYILNLNRLGLVLLVLHYFIELLFHVSRLVYFSNENRQMGFTIWAVLFVLGRLLTLSLSVLTVGFGLATAENQGFDLAAGNFNILFVRITVLAAICLTQAFMMWKFINFQLRRWREHAQAQTLKKKQVSSKSKSKKDKANGVNGVNSHGADSPRARKEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A872</accession>
    <name>A0A673A872_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">NEDD4-binding protein 3-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">n4bp3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the N4BP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A872"/>
    <dbReference type="Ensembl" id="ENSSORT00005025514.1">
        <property type="protein sequence ID" value="ENSSORP00005024782.1"/>
        <property type="gene ID" value="ENSSORG00005011924.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A872"/>
    <dbReference type="OrthoDB" id="10030037at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033571">
        <property type="entry name" value="N4BP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32274">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32274:SF1">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06818">
        <property type="entry name" value="Fez1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="185"/>
            <end position="245"/>
        </location>
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        <location>
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            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <end position="522"/>
        </location>
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            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="188"/>
            <end position="206"/>
        </location>
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            <begin position="224"/>
            <end position="233"/>
        </location>
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        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="584"/>
            <end position="594"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="646" mass="71768" checksum="C657F6CE925CDF7F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A902</accession>
    <name>A0A673A902_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A902"/>
    <dbReference type="Ensembl" id="ENSSORT00005026539.1">
        <property type="protein sequence ID" value="ENSSORP00005025771.1"/>
        <property type="gene ID" value="ENSSORG00005012386.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A902"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050868">
        <property type="term" value="P:negative regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032748">
        <property type="entry name" value="PAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16322:SF1">
        <property type="entry name" value="LCK-INTERACTING TRANSMEMBRANE ADAPTER 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16322">
        <property type="entry name" value="PHOSPHOPROTEIN ASSOCIATED WITH GLYCOSPHINGOLIPID-ENRICHED MICRODOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="99" mass="10717" checksum="8EC2E89BA815B90C" modified="2020-06-17" version="1">MSESVMDENEDNGYGDPPYKNLLLLGAIAAASAFVVTILIVLVCVGCQRKSKSKHPPAGEKGTSVNMQGTLRHPNLNSMSKSDTRLHEINRFPCNGNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A949</accession>
    <name>A0A673A949_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Inositol polyphosphate-5-phosphatase Kb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">inpp5kb</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A949"/>
    <dbReference type="Ensembl" id="ENSSORT00005026866.1">
        <property type="protein sequence ID" value="ENSSORP00005026095.1"/>
        <property type="gene ID" value="ENSSORG00005012538.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A949"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016312">
        <property type="term" value="F:inositol bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
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    </dbReference>
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        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
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        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="445" mass="51148" checksum="B72A39BDDBB9E0C5" modified="2020-06-17" version="1">MVTWNVATAEPPEDVTSLLQLDVQPPTDLYVIGLQEVNATPVRFISDLIVEDSWSHVFMDTLAPRGFVKVTSVRMQGLLLLIFAKQVHLPFIRNIQSTYTRTGIFGYWGNKGGVSVRFSFYGHMVCFVNCHLAAHMNNALQRVDEFEYILDTQDFDHADTPHVLDHKVVFWFGDLNFRIADHGLHFLRSSINGGRLNLLWNKDQLTMMKSKEPFLQEFDEGPLNFKPTYKFDRNSDTYDTSGKKRKPAWTDRILWRIKPKTLPSEDDDERASTSGDDGYDDYPIMVTQDKYTSDMTYGVSDHKPVIATFSVELRKCFDTPLVHVSPEGVWSADHDALLNYTVQEDFMSSTWDWIGLYKVGFKSPSDYETFVWVREDDLPETNEVIQLTVDKDDIPLLGGQYVLGYYSTNMQSIIGLSANFQVSMYSCVIFLHEYYTSNPEEVEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AA46</accession>
    <name>A0A673AA46_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Chromosome 11 open reading frame 96</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">c6h11orf96</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Neoteleostei</taxon>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSSORG00005012533.1"/>
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        <property type="entry name" value="CHROMOSOME 11 OPEN READING FRAME 96"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SI:CH1073-281M9.1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025331350" evidence="2">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
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        <location>
            <begin position="44"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="139" mass="15291" checksum="A0EFAC38D107E268" modified="2020-06-17" version="1">FSFGFCVLAGVCVPMAAVRQMVVEPSGFHVLPAHLMASAMEEFPQQLPVPKAPSRGKSRSRRPRDARFKTQPVTFAEIAEVEEEGSSPLEEERARRSFLQSLENLRRSTQTLHCSAPPTQHTSTSTQTAMDSSDSDSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAT2</accession>
    <name>A0A673AAT2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSSORP00005025843.1"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026768.1">
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        <property type="gene ID" value="ENSSORG00005012487.1"/>
    </dbReference>
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        <property type="entry name" value="GEX1/Brambleberry"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538:SF2">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627717" evidence="2">
        <location>
            <begin position="34"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="315" mass="35865" checksum="130551479BF4E983" modified="2020-06-17" version="1">YLMLNWWFSAHFYMVKVPLTQTLLLLLFLPVSAGTGPDPDRGRTELRRVQSLAAQPRYGDCWARALEHLDTRCRDMTSESQSRIALRFTHCHLSSSGRDFPSCPEGSDVRRCTASMDAVAFNTYTEFFTHTHSICHFLQSEAWQSRAENTMHRLTESSAGVAEQLQSTRQMAEDLIEAQSAALQAQQEILDNGEELRVTLRDSTQGLRSIFLELSSVSREQQVALSELFNRVSFLQSFLMVEAHSMSSCLYNAAALCASFLLTSTQRSSRARYLLLLLTFMNILLGLYILLYIGVQNREILNKHQDVTRKKHPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGQ2</accession>
    <name>A0A673AGQ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Outer dense fiber protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cimap1b</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AGQ2"/>
    <dbReference type="FunCoup" id="A0A673AGQ2">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029292.1">
        <property type="protein sequence ID" value="ENSSORP00005028481.1"/>
        <property type="gene ID" value="ENSSORG00005013621.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGQ2"/>
    <dbReference type="OrthoDB" id="429991at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF28">
        <property type="entry name" value="BOREALIN N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="256" mass="27000" checksum="11439868E77BDAC7" modified="2020-06-17" version="1">MTSADPWVGTWRPHRPKGPIAALHGSPGPAYALPGLTGGERHDPTKNKAPVISLGKRYELPSFNCSPGPKYLVPANVSKTGRHGTPAFSLHSRLKSPKVVKTPGPGHYSPEPSGKSVFRSCPAYSVCGRSKDMSRDETPGPGSYNLPTALGCKTVVTSAAPAHSISSRSKIGGFNEDLKKSPGPAAYKVVDSCIFKSKPPQYSMTGRNFTPSPGANTPGPGSHNPGKVTFTKAKAPCFSFGLRHSEYILPVPNVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AH79</accession>
    <name>A0A673AH79_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Plasmalemma vesicle associated protein b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">plvapb</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AH79"/>
    <dbReference type="FunCoup" id="A0A673AH79">
        <property type="interactions" value="528"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029880.1">
        <property type="protein sequence ID" value="ENSSORP00005029060.1"/>
        <property type="gene ID" value="ENSSORG00005013870.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AH79"/>
    <dbReference type="OrthoDB" id="9944409at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002693">
        <property type="term" value="P:positive regulation of cellular extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043114">
        <property type="term" value="P:regulation of vascular permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009538">
        <property type="entry name" value="PV-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21687">
        <property type="entry name" value="PLASMALEMMA VESICLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21687:SF6">
        <property type="entry name" value="PLASMALEMMA VESICLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="389"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="48718" checksum="E8D70FC2F36A574F" modified="2020-06-17" version="1">MYSSSYSRAKFGLESREPLHRPRGKSCGYYLRIIFFFSSLIQSLIIVSLVLFLIYGQPEKSAAEKRVKELELNFNRLSEYNIQLRKEKGELGDQLGARTAEKAALEKEMENLKTAANNTEHELLKKLADCERQVAVTRTMVVRCPTQAMAPPPMVTSGSEMKTLQTLNAQQRAFINLIEANFTQTVHYLTQERDNALKDRDTHHQDAIAQRRDNTMLKEQLVTYTRKCKEDFAHSLDGIKMVTSEFLNKINNLFPHQLTFHLTCDSQQEQMEKIRTSCTNLSRDVENKFQLYLDTVGNKVSQIQAQSSRLEVQNSHLTADFQKCEQSRSEAISDASKQLELKQKKHDDQVERLLMEQNRLREQKKLQEDTLALKETEFKTLLLSAQQNCKPAAPKPMGPQLVPPFNRQTVPNWPAPGIGKTPTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJL0</accession>
    <name>A0A673AJL0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Growth arrest-specific protein 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115410819</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029978501.1">
        <property type="nucleotide sequence ID" value="XM_030122641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AJL0"/>
    <dbReference type="Ensembl" id="ENSSORT00005030292.1">
        <property type="protein sequence ID" value="ENSSORP00005029459.1"/>
        <property type="gene ID" value="ENSSORG00005013444.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410819"/>
    <dbReference type="CTD" id="100333627"/>
    <dbReference type="InParanoid" id="A0A673AJL0"/>
    <dbReference type="OrthoDB" id="28357at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07649">
        <property type="entry name" value="F-BAR_GAS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11829">
        <property type="entry name" value="SH3_GAS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000216">
        <property type="entry name" value="growth arrest-specific protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000024">
        <property type="entry name" value="growth arrest-specific protein 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037957">
        <property type="entry name" value="GAS7_F-BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF57">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16623">
        <property type="entry name" value="WW_FCH_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="F-BAR" evidence="8">
        <location>
            <begin position="195"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="97"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="307"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="475" mass="54694" checksum="CA3887136B21215A" modified="2020-06-17" version="1">MSGSYCKSLYPFSGEQHQQGLSFDTGEVIKVVQALPGGWWEGEKDGARGWFPSSYVQVLEKTASLSQLPVEDLRDPLPPQWRCYMSPQGRRYYVNTTNNETTWERPSSAPGTPKTSLRHKNSLPAVNGFHSGGSPLHHVEHSHNSLVRKSSTEPQQSPGSTSPTRKQNKETTVTINCVTFPSPVSLVEQQLLKPNEWSYCDYFWTDKKDPQGNGSITGFEVLLQKQLKGKQMQKEMAEFIRERIKIEEEYAKNLSKLSQIPLASQEEGTLGEAWAQLKKSLADEAEVHLKFSSKLQSEVEKPFLTFRENFKKDMKRFDHHIADLRKQLVGRYAAVEKARKALADRQKELELKTQQLEIKLSNKIEEDIKKARRKSTQAGDDLMRCVDLYNQSQSKWFEEMVTTSLELERLEVERIEMIRQHLCQYTTLRHETDMFNQSTMEPVDQLLQCVDPGKDRELWVRENKTGEIRPVDIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AK89</accession>
    <name>A0A673AK89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fizzy-related protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cdh1/Hct1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115437763</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016956.1">
        <property type="nucleotide sequence ID" value="XM_030161096.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030016957.1">
        <property type="nucleotide sequence ID" value="XM_030161097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AK89"/>
    <dbReference type="FunCoup" id="A0A673AK89">
        <property type="interactions" value="2012"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029898.1">
        <property type="protein sequence ID" value="ENSSORP00005029078.1"/>
        <property type="gene ID" value="ENSSORG00005013877.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437763"/>
    <dbReference type="InParanoid" id="A0A673AK89"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000025">
        <property type="entry name" value="FIZZY-related 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF34">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="12">
        <location>
            <begin position="182"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="442"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="496" mass="54817" checksum="454AA2D8842FD04F" modified="2020-06-17" version="1">MDQDYECRLLRQINIQNENASPIKAVGAVRAMTPTSSPLSSPSKHGDRFIPSRAGANWSVNFHRINEIEKSHNQNRKTKDGTTDTNKADGLAYSALLKNELLGAGIEKVQDPQSEDRRLQPSTPAKRSLFSYSVSTKRTLPEEDGNTVSPYSLSPVSSNSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGYVQIWDAAAGKKLSVLEGHTARVGALAWNADQLSSGSRDRVILQRDIRAPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSVLPVQQYTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCTDTGSQVCNLAWSKHTNELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKMRSTKESVSVLNLFTRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALR1</accession>
    <name>A0A673ALR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein artemis</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA cross-link repair 1C protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">dclre1c</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ALR1"/>
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        <property type="interactions" value="376"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031140.1">
        <property type="protein sequence ID" value="ENSSORP00005030291.1"/>
        <property type="gene ID" value="ENSSORG00005014446.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12650:FF:000002">
        <property type="entry name" value="DNA cross-link repair 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000018">
        <property type="entry name" value="DNA cross-link repair 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF8">
        <property type="entry name" value="PROTEIN ARTEMIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="15">
        <location>
            <begin position="230"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="404"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="489"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="529"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="561"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="632" mass="72065" checksum="0079873CA92F0E90" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AP28</accession>
    <name>A0A673AP28_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">APC membrane recruitment protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein FAM123A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">amer2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Negative regulator of the canonical Wnt signaling pathway involved in neuroectodermal patterning. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Amer family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AP28"/>
    <dbReference type="FunCoup" id="A0A673AP28">
        <property type="interactions" value="650"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031017.1">
        <property type="protein sequence ID" value="ENSSORP00005030172.1"/>
        <property type="gene ID" value="ENSSORG00005014403.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AP28"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019003">
        <property type="entry name" value="AMER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22237:SF1">
        <property type="entry name" value="APC MEMBRANE RECRUITMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22237">
        <property type="entry name" value="APC MEMBRANE RECRUITMENT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09422">
        <property type="entry name" value="AMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="337"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="508"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="570" mass="60601" checksum="268B9D9634E620C7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQ86</accession>
    <name>A0A673AQ86_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Asparaginyl-tRNA synthetase 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AQ86"/>
    <dbReference type="Ensembl" id="ENSSORT00005032301.1">
        <property type="protein sequence ID" value="ENSSORP00005031424.1"/>
        <property type="gene ID" value="ENSSORG00005014943.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032307.1">
        <property type="protein sequence ID" value="ENSSORP00005031429.1"/>
        <property type="gene ID" value="ENSSORG00005014949.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="6">
        <location>
            <begin position="12"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="247" mass="28203" checksum="FE27D6059E5B3C6A" modified="2020-06-17" version="1">MLCPLYSRAFSRVYTFGPTFRAENSQSRRHLAEFYMVEAEVSFTQSLEDLTKVMEDMFRSATEHVLAQCADDVDLFHKHVTPGHRVMVLFPGSCLFLPTELVTIINSSFSPQWGCDLQTEHEKYLVKHCGNIPVFVTDYPYALKPFYARDNQDRPNHTAAAVDLLVPGVGELCGGSLREERLHLLQSRLEQVGLEDIYSWYLDLRRFGSVPHGGFGLGFERYLQCILGVDNIKDVIPFPRFSRSCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARE2</accession>
    <name>A0A673ARE2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Epithelial membrane protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115421914</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PMP-22/EMP/MP20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993780.1">
        <property type="nucleotide sequence ID" value="XM_030137920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ARE2"/>
    <dbReference type="FunCoup" id="A0A673ARE2">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032957.1">
        <property type="protein sequence ID" value="ENSSORP00005032071.1"/>
        <property type="gene ID" value="ENSSORG00005015236.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421914"/>
    <dbReference type="CTD" id="560722"/>
    <dbReference type="InParanoid" id="A0A673ARE2"/>
    <dbReference type="OrthoDB" id="8714888at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004032">
        <property type="entry name" value="PMP22_EMP_MP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF8">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01453">
        <property type="entry name" value="EPMEMFAMILY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01221">
        <property type="entry name" value="PMP22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025425713" evidence="7">
        <location>
            <begin position="18"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="165" mass="18899" checksum="452A9ECE8618EABF" modified="2020-06-17" version="1">MAYLLMFVTLLHLITLAMLFIATMEKSWWVWDGTVSSDLWYNCRFDNFTGTWLCASSKETEWLQAVQVLMVLSVVFSSVSFLVFLGQLFTMSKGGLFYFTGLCQIFAGLTAFSAAVIYTLHYKEILQDSRELTSGRFGYCFILAWVCVPLLLCSGIIYIHLRKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASG2</accession>
    <name>A0A673ASG2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">si:ch211-283g2.1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ASG2"/>
    <dbReference type="Ensembl" id="ENSSORT00005033502.1">
        <property type="protein sequence ID" value="ENSSORP00005032605.1"/>
        <property type="gene ID" value="ENSSORG00005015477.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASG2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF306">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="370"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="415"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="446"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="577" mass="64493" checksum="7DAD28A0F39E8DA4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWU8</accession>
    <name>A0A673AWU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcium binding protein 39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cab39</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AWU8"/>
    <dbReference type="Ensembl" id="ENSSORT00005034652.1">
        <property type="protein sequence ID" value="ENSSORP00005033739.1"/>
        <property type="gene ID" value="ENSSORG00005015972.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AWU8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000025">
        <property type="entry name" value="Calcium-binding protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF11">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="40195" checksum="EF49F38AFC1C35D4" modified="2020-06-17" version="1">LSTMPFPFGKSHKSPADIVKNLKDSMTVLEKHDISDKKAEKATEEVSKSLVAMKEILYGTNEKEPQTEAVAQLAQELYNSGLLSTLIADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYLCTQQNILFMLLKGYESPEIALNCGIMLRECIRHEPLAKITLWSEQFYDFFRYVEMSTFDIASDAFATFKDLLTRHKLLSAEFLEQHYDRFFSEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIMTKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQTKLIEFLSKFQNDRTEDEQFNDEKTYLVKQIRDLKRPPPQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1Z6</accession>
    <name>A0A673B1Z6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">dapk2a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673B1Z6"/>
    <dbReference type="FunCoup" id="A0A673B1Z6">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037202.1">
        <property type="protein sequence ID" value="ENSSORP00005036241.1"/>
        <property type="gene ID" value="ENSSORG00005017100.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B1Z6"/>
    <dbReference type="OrthoDB" id="74764at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000110">
        <property type="entry name" value="Death-associated kinase 3, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000250">
        <property type="entry name" value="Death-associated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF15">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="13"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="277"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="494" mass="56896" checksum="5D595C6851DD5355" modified="2020-06-17" version="1">MAVFKQQRVEDFYEIGEELGSGQFAIVKQCQEKSTGLEFAAKFIKKRQSMASSRGVRREEIEREVDILQQIQHPNIVTLHDVYENRTDVVLILELVSGGELFDFLAQKESLSEEEATQFIKQILEGVNYLHARKIAHFDLKPENIMLLDKNVPLPRIKLIDFGLAHKIEAGVEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQDTLRNISAINYDFDEEFFCHTSELAKKFISQLLERDKKKRLTIQDALNHPWIKSNEHKEENKAQEPKKRERRQLKTKRLREYTIKSHSSMPPNNTYVNFERFAQVVEDIDQMESSFISLAAAHDSLQEDIDAMVSIYNEKEAWYKEESEEVRHELSQIRYEFRKVEALKRSLQDDMQAFSCSLDTISGQYQERQNHFDALRLELSNELKWVHEVVGSFPMDGGGGGYPNCSFSTVFNNDVNEALKELLNRSCGGELLSGINLDFSETGQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5Q0</accession>
    <name>A0A673B5Q0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B5Q0"/>
    <dbReference type="Ensembl" id="ENSSORT00005037630.1">
        <property type="protein sequence ID" value="ENSSORP00005036664.1"/>
        <property type="gene ID" value="ENSSORG00005017263.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5Q0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08574">
        <property type="entry name" value="GDPD_GDE_2_3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344:SF13">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="45"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="347" mass="38275" checksum="D681839D99BE8C4B" modified="2020-06-17" version="1">LNGSIGRFTVAVLGLFLSVLFSLYLVPLGMYSPCIKEAGTLGPAPALIGHRGAPMLAPENTLMSFEKAVEAGGAGLETDVTISYDGVPFLMHDTTLKRTTNVADVFPNQTHQDASMFTWAELQQLNAGDWFLSRDPFGTVSSMSEMDRAWAQNQSVPSLAQFLQVAARSGRLVLFDLRMPPYGHPYSQTYINTTLQVVKANINSSQVLWLPSADRESVQAMDPDLQQTSGEKASIQELMDGHITRLNLHYSTMSQQQISKYRSVNITTNLYVISQPWLYTLGWCAGAQSVTTNSVHVLSGITKPLFLMTPEDYNLMWILTDAVSAFLIISIFIFHWCVDSLDALSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5X2</accession>
    <name>A0A673B5X2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Innate immunity activator protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">inavab</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B5X2"/>
    <dbReference type="FunCoup" id="A0A673B5X2">
        <property type="interactions" value="803"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038679.1">
        <property type="protein sequence ID" value="ENSSORP00005037696.1"/>
        <property type="gene ID" value="ENSSORG00005017691.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5X2"/>
    <dbReference type="OrthoDB" id="10063592at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034334">
        <property type="term" value="P:adherens junction maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043447">
        <property type="entry name" value="CCDC120/INAVA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021774">
        <property type="entry name" value="CUPID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16093">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 120 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16093:SF6">
        <property type="entry name" value="INNATE IMMUNITY ACTIVATOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11819">
        <property type="entry name" value="CUPID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytohesin Ubiquitin Protein Inducing" evidence="5">
        <location>
            <begin position="19"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="188"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="369"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="568"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="616"/>
            <end position="629"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="635" mass="71136" checksum="34461472D1464017" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B660</accession>
    <name>A0A673B660_9TELE</name>
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        <recommendedName>
            <fullName evidence="15">Visual pigment-like receptor peropsin</fullName>
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    </protein>
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        <name evidence="19" type="primary">rrh</name>
        <name evidence="20" type="synonym">LOC115415948</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="19" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">May play a role in rpe physiology either by detecting light directly or by monitoring the concentration of retinoids or other photoreceptor-derived compounds.</text>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
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            <dbReference type="Proteomes" id="UP000472271"/>
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    <sequence length="336" mass="37005" checksum="1ECC44A73DABE07D" modified="2020-06-17" version="1">MGMDSEVNTSTAVAEYGGKSAFTQLEHNIVAGYLITAGVISLSSNIVVLLMFVKFKELRTATNSIIINLAFTDIGVAGIGYPMSAASDIHGSWKFGYTGCQIYAALNIFFGMASIGLLTVVAIDRYLTICRPDIGQKMTVRSYNLLIAAAWLNAVFWSSMPVVGWAGYAPDPTGATCTINWRHNDASFISYTMTVIAVNFVLPLSIMFYCYYHVSVTVKRTRASNCLDSVNVDWSDQMDVTKMSVVMILMFLVAWSPYSLVCLWASFGDPGSIPASMAIIAPLFAKSSTFYNPCIYVVANRKFRRAIIGMIRCQTRQRITINTQVPMTMSQQPLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B674</accession>
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        <submittedName>
            <fullName evidence="8">Synaptic Ras GTPase activating protein 1b</fullName>
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        <name evidence="8" type="primary">syngap1b</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="268"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="293"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="7">
        <location>
            <begin position="478"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="774"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="821"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="920"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="992"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1084"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1279"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="781"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="843"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="927"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1084"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1100"/>
            <end position="1119"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1126"/>
            <end position="1135"/>
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        <location>
            <begin position="1136"/>
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        <location>
            <begin position="1149"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1194"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1224"/>
            <end position="1234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1407" mass="157490" checksum="180C3402C580D6F3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B7V1</accession>
    <name>A0A673B7V1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribonuclease P protein subunit p30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RNase P subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rpp30</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a catalytic RNA moiety and about 10 protein subunits; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
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        <property type="gene ID" value="ENSSORG00005017939.1"/>
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        <property type="component" value="Chromosome 19"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:tRNA processing"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="RNase_P_p30"/>
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    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
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    </dbReference>
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            <end position="303"/>
        </location>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068480"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="303" mass="33317" checksum="CC9C974BEBC50A93" modified="2020-06-17" version="1">MLMFTFVTKNSSSRHASRLLTCGGPCTVSIMSVFMDLNLIQNQTEDRSGLKSLIETAAHLGFSTVAVNYVFEPTTKKKQNIPSPTPINELIDQLPIVQGRSRPIRVLNRLTIVMSDSSHYRPTAAEYRRFDLLAVYPTTEKLFHAACMTYDVDIICISVTEKLPFFFKRAPVNGAIERGVAFEVSYSAAIRDATMRRYTIANAIALVDTCKGKNVIVSSAAEKRLELRGPHDAANLSLLFGLSDADAKEAVSSTCRSVVLHAERRKSAGGIIHTTKTSPEETPPTPDDRDAPAAKRAKPLETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B7Z3</accession>
    <name>A0A673B7Z3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Insulin-like growth factor 2 mRNA binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">igf2bp3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM IMP/VICKZ family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B7Z3"/>
    <dbReference type="SMR" id="A0A673B7Z3"/>
    <dbReference type="FunCoup" id="A0A673B7Z3">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037688.1">
        <property type="protein sequence ID" value="ENSSORP00005036722.1"/>
        <property type="gene ID" value="ENSSORG00005017294.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B7Z3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22490">
        <property type="entry name" value="KH-I_IGF2BP1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000203">
        <property type="entry name" value="insulin-like growth factor 2 mRNA-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000026">
        <property type="entry name" value="Insulin-like growth factor 2 mRNA-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000027">
        <property type="entry name" value="insulin-like growth factor 2 mRNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="81"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="593" mass="64465" checksum="03ADCA44BC913DC8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9G9</accession>
    <name>A0A673B9G9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Synaptogyrin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115416545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptogyrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B9G9"/>
    <dbReference type="Ensembl" id="ENSSORT00005039467.1">
        <property type="protein sequence ID" value="ENSSORP00005038475.1"/>
        <property type="gene ID" value="ENSSORG00005017971.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B9G9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016579">
        <property type="entry name" value="Synaptogyrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838:SF7">
        <property type="entry name" value="SYNAPTOGYRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="8">
        <location>
            <begin position="56"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="252" mass="27099" checksum="CF31803C3DAF665B" modified="2020-06-17" version="1">MVLALVLVVVLVLVLVLILVLALVLVLVLTLSPLHPQLFLVIIFGCVTGSGSGSGASLGSNSGPGSGSDLFSVVIFGCVANEGYVNRPEEEAEFCIFNRNQSACNYAVAMGTLCFLCSSAFLALDVYFPQISGVKDRKKAVMADIGASAMWALLWFVGFCFLANQWQVSREEDNPLREGADAARAAIVFSFFSVFTWSVQCLLAVHRFKLGSDSVLFNQDYVDPNQQEAPQAPPTEDQMGGAMLHMATPTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCR7</accession>
    <name>A0A673BCR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Activator of basal transcription 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">abt1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029994755.1">
        <property type="nucleotide sequence ID" value="XM_030138895.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029994757.1">
        <property type="nucleotide sequence ID" value="XM_030138897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BCR7"/>
    <dbReference type="Ensembl" id="ENSSORT00005039403.1">
        <property type="protein sequence ID" value="ENSSORP00005038411.1"/>
        <property type="gene ID" value="ENSSORG00005017945.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039404.1">
        <property type="protein sequence ID" value="ENSSORP00005038412.1"/>
        <property type="gene ID" value="ENSSORG00005017945.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422529"/>
    <dbReference type="CTD" id="29777"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="304" mass="35784" checksum="2B7CDC1A946F8388" modified="2020-06-17" version="1">MEKRDEEELKKTETDREKEEEEGDEEEEVKEPAAQMEEEEEGGEGATDRNDGDDDGDEDAKKSLKKKPKLKVGGAKDSKCIPGIIYLGHIPPRLRPKHLRNLLSVYGEIGRIFLQPEDRTVRMKKKKSGLRRCDFTEGWVEFRDKRVAKRVAASLHNTPMGTRKRQRFASDLWCIKYLHRFQWTHLSERLAYEHTVLQQRLRTEVSQAKRETNFYLNNVDKGAHMDKDRRKRQRDGAQVDEKTWDFTQRRTEDEIQTMKKKKKQQQGQRDSITQKRLDKARLIEQKSQSNVSLLAKIFNSKQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BE48</accession>
    <name>A0A673BE48_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BE48"/>
    <dbReference type="Ensembl" id="ENSSORT00005041081.1">
        <property type="protein sequence ID" value="ENSSORP00005040045.1"/>
        <property type="gene ID" value="ENSSORG00005018681.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BE48"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02389">
        <property type="entry name" value="Cornifin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="227" mass="21677" checksum="C6C6237E397ABFD4" modified="2020-06-17" version="1">SVPESSTGSVPESSLGSVPESSTGSVPESCMGSVPESSTGSVPESSLGSVPESCMGSVPESSTGSVPESSLGSVPESCMGSVPESSTGSVPESSTGSVPESCMGSVPESSTGSVPESSLGSVPESCSGSVPESCMGSVPESSLGSVPESSTGSVPESCMGSVPESSTGSVPESCMGSVPESCMGSVPESSTGSVPESSLGSVPESCSGSVPESSMGSVPESCTGSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFK6</accession>
    <name>A0A673BFK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pitx3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BFK6"/>
    <dbReference type="FunCoup" id="A0A673BFK6">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040401.1">
        <property type="protein sequence ID" value="ENSSORP00005039392.1"/>
        <property type="gene ID" value="ENSSORG00005018384.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BFK6"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021536">
        <property type="term" value="P:diencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000031">
        <property type="entry name" value="Homeobox protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016233">
        <property type="entry name" value="Homeobox_Pitx/unc30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882:SF2">
        <property type="entry name" value="PITUITARY HOMEOBOX 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000563">
        <property type="entry name" value="Homeobox_protein_Pitx/Unc30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="58"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="14">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="60"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="294" mass="32631" checksum="A6C3836F9204F3FC" modified="2020-06-17" version="1">MDFNLLTDSEARSPALSLSDSGTPQHEQGCKGQEHSDTEKSLQNQLDESSAEDGALKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGAQFNGLMQPYDDMYTGYSYNNWATKSLASSPLSAKSFPFFNSMNVSPLSSQPMFSPPSSIPSMNMASGMVPSAVTGVPAAGLNNLGNLNNLNSPTALNSVAVTAATCPYATSASPYMYRDTCNSSLASLRLKAKQHTNFAYPAVQNPVSNLSPCQYAVDRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BG43</accession>
    <name>A0A673BG43_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Complement component 8, gamma polypeptide</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">c8g</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BG43"/>
    <dbReference type="FunCoup" id="A0A673BG43">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040603.1">
        <property type="protein sequence ID" value="ENSSORP00005039592.1"/>
        <property type="gene ID" value="ENSSORG00005018459.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BG43"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001848">
        <property type="term" value="F:complement binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006956">
        <property type="term" value="P:complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043245">
        <property type="entry name" value="C8G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47304">
        <property type="entry name" value="COMPLEMENT COMPONENT C8 GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47304:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT C8 GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025426184" evidence="1">
        <location>
            <begin position="25"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="61"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="226" mass="25565" checksum="0687DECBE8F39AAF" modified="2020-06-17" version="1">MAGVWRYMLAVVVICVCLWGFSEAVGGAVSRPRPQRRPRKPKVAPVTVTPPVQSIDIQQMTGRWYLLNTASKCSYLIKHGTKVEPTVMTLTLPSSSNQTLSVSTKTRYNHQCWEILQVYDLTSTAGRLTLKGTRPELNTEIVIGDTDYSSYAIIYYHKRDKVTMKLYGRSVDNLSEPTLSKFEERAEQQNLGLAYLFPFPTYSHCGDVDQDHVISELKHFLLSVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BG45</accession>
    <name>A0A673BG45_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BG45"/>
    <dbReference type="Ensembl" id="ENSSORT00005041301.1">
        <property type="protein sequence ID" value="ENSSORP00005040264.1"/>
        <property type="gene ID" value="ENSSORG00005018784.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BG45"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="374" mass="37697" checksum="6BE891F3D8F600D1" modified="2020-06-17" version="1">MFTSSLAIIKSHIKASGKKSPSTDISAAIRGMCVSLYTRYKEHLKQRREKVNEQKELKLKQAVKVLQAIRLKKVNKASNSAPARASTSSDAARASTSSAPARASTSSAPARASTSSDAARASTSSAPARASTSSAPARASTSSAPARASTSSDAARASTSSAPARASTSSAPARASTSSAPARASTSSDAARASTSSAPARASTSSAPARASTSSDAARASTSSAPARASTSSDAARASTSSAPARSSTSSAPARASTSSDAARASTSSAPTRSSTSSAPARASTSSAPARASTSSAPSSTVPATSSTAKVKSSTKRPPDPPPIPLMTFFSIHVTNLLHLYTLPFSVFYSISKFDYINLYAIVVLYCIILNGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BGI8</accession>
    <name>A0A673BGI8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">SPARC related modular calcium binding 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">smoc2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030012765.1">
        <property type="nucleotide sequence ID" value="XM_030156905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BGI8"/>
    <dbReference type="Ensembl" id="ENSSORT00005042746.1">
        <property type="protein sequence ID" value="ENSSORP00005041676.1"/>
        <property type="gene ID" value="ENSSORG00005019368.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434772"/>
    <dbReference type="CTD" id="64094"/>
    <dbReference type="InParanoid" id="A0A673BGI8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16241">
        <property type="entry name" value="EFh_SPARC_SMOC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000076">
        <property type="entry name" value="SPARC-related modular calcium binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000004">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000003">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037640">
        <property type="entry name" value="SMOC2_EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF21">
        <property type="entry name" value="SPARC-RELATED MODULAR CALCIUM-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16597">
        <property type="entry name" value="Thyroglob_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025496578" description="SPARC related modular calcium binding 2" evidence="11">
        <location>
            <begin position="19"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="13">
        <location>
            <begin position="29"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="12">
        <location>
            <begin position="81"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="12">
        <location>
            <begin position="205"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="118"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="243"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="48862" checksum="AE0691C6720622C5" modified="2020-06-17" version="1">MRVCPVLLFLCLVCAGSTQKFSALTFLRVDQDKECNMECSGAPRKPLCASDGRTFTSRCEFLRAKCRDPQLEVIRGPCKDTSRCVAEKKYTEQQAKKLFPQVFVPVCNPDGTYSEVQCHSYTGYCWCVTPNGRPISGSAVANKKPRCQGSKQERATTREPGKADETGLVVDPQPPADEEDIISQYPTLWTEQVRSRQNNRTRAPSSSCDQEQQSAQEEARQHKNDAVFVPDCAQGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPKCDGNARAHPTKPKDHYRSRHLQGCPGAKKTEFLTSVLDALSTDMVHAVTDPASAGRMAEPDPNHTLEERVVHWYFSQLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDISNDKALSLQELMGCLGVTKEEESFKEARLSQQQSVPLPHGDSALTNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLU7</accession>
    <name>A0A673BLU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SKICH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BLU7"/>
    <dbReference type="Ensembl" id="ENSSORT00005042290.1">
        <property type="protein sequence ID" value="ENSSORP00005041228.1"/>
        <property type="gene ID" value="ENSSORG00005019207.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLU7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041611">
        <property type="entry name" value="SKICH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051002">
        <property type="entry name" value="UBA_autophagy_assoc_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31915:SF5">
        <property type="entry name" value="CALCIUM-BINDING AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31915">
        <property type="entry name" value="SKICH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17751">
        <property type="entry name" value="SKICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKICH" evidence="2">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="108" mass="12416" checksum="FDA9DA2FC602F320" modified="2020-06-17" version="1">YRLSSAVLLQTDAEATVVFRNVGQLYFPQTRVECHYSLTSEHQWSNSDWIGIFEVGWSTVKEYYTYTWALVPENYTEGTNVNCCALFHGESLAMKPQKILQNVTNIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQR4</accession>
    <name>A0A673BQR4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSSORP00005044120.1"/>
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        <property type="component" value="Chromosome 17"/>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="87" mass="10630" checksum="70DA011B362E4D13" modified="2020-06-17" version="1">MFVFIDAYLYGYINVYRYFCVYILVFIDTFVVYRYICVYIFVFIDTIVFIDTYISVYTYICGYIFVFTDTLVFTDTYVVIYLCLQIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQU0</accession>
    <name>A0A673BQU0_9TELE</name>
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        <recommendedName>
            <fullName evidence="16">Adhesion G protein-coupled receptor F5-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
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        <property type="protein sequence ID" value="ENSSORP00005042573.1"/>
        <property type="gene ID" value="ENSSORG00005019725.1"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Adhesion G protein-coupled receptor F5"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="ADGRF3/5_N"/>
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    <dbReference type="InterPro" id="IPR051587">
        <property type="entry name" value="Adhesion_GPCR"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
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        <property type="entry name" value="GPCR_2_Ig-hepta-like_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813:SF4">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25387">
        <property type="entry name" value="ADGRF3_N"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPS"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1172"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1212"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1253"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1290"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1337"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1382"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="630"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="750"/>
            <end position="839"/>
        </location>
    </feature>
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        <location>
            <begin position="1003"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="12">
        <location>
            <begin position="1170"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="494"/>
            <end position="542"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1444" mass="158110" checksum="62BD1807450BCF7F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQY2</accession>
    <name>A0A673BQY2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">RAB39B, member RAS oncogene family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rab39bb</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Autolysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
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            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
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    <dbReference type="FunCoup" id="A0A673BQY2">
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    <dbReference type="Ensembl" id="ENSSORT00005045310.1">
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    </dbReference>
    <dbReference type="GO" id="GO:0120281">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RAB39B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041818">
        <property type="entry name" value="Rab39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24398" checksum="A88C2B40A38E43C5" modified="2020-06-17" version="1">MEAIWLYQFRLIVIGDSTVGKSCLIRRFTEGRFAQVSDPTVGVDFFSRLVEIEPGKRIKLQIWDTAGQERFRSITRAYYRNSVGGLLLFDITNRRSFQNVHDWLEEARSHVQPHSIVFLLVGHKCDLEAQRQVTRQEAEKLAGAYGMRYVETSARDAINVEHAFTELTRDIFALVRSGDITIQEGWEGVKSGFVPNVVHSSEEVTKSDRRCLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQZ4</accession>
    <name>A0A673BQZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat and BTB/POZ domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">abtb2b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May be involved in the initiation of hepatocyte growth.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BQZ4"/>
    <dbReference type="Ensembl" id="ENSSORT00005043709.1">
        <property type="protein sequence ID" value="ENSSORP00005042623.1"/>
        <property type="gene ID" value="ENSSORG00005019508.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQZ4"/>
    <dbReference type="OrthoDB" id="2316821at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22913">
        <property type="entry name" value="HFD_ABTB2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000057">
        <property type="entry name" value="ankyrin repeat and BTB/POZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000045">
        <property type="entry name" value="Ankyrin repeat and BTB/POZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000030">
        <property type="entry name" value="Ankyrin repeat and BTB/POZ domain-containing protein BTBD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059008">
        <property type="entry name" value="ABTB2/3_histone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052089">
        <property type="entry name" value="Ankyrin-BTB/POZ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46071">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46071:SF3">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB_POZ DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26281">
        <property type="entry name" value="Histone_ABTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="523"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="569"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="608"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="863"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1054" mass="115863" checksum="3915C11307F7051A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSV3</accession>
    <name>A0A673BSV3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THAP domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">thap5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BSV3"/>
    <dbReference type="Ensembl" id="ENSSORT00005045998.1">
        <property type="protein sequence ID" value="ENSSORP00005044865.1"/>
        <property type="gene ID" value="ENSSORG00005020626.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BSV3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052224">
        <property type="entry name" value="THAP_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927">
        <property type="entry name" value="AGAP005574-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927:SF1">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="351" mass="39852" checksum="A070BFD8AB8EDD98" modified="2020-06-17" version="1">MPRYCAVKVCRNRGVHASRQESKRISFYPLQKWVDNMNREKWTPSRHQFLCSEHFTEDCFDIRWGIRYLKNTAIPTIFPPVEDVSTTDSDKKSSNIKRSPKARPGLLDSDIDPTLSEYSRRKKPLILSRTCKKVSSNMTTHNVVAEAKEKTEAIHAEGLELPLAPDSVVTCHSNIPEPQTAKSGVPGDTVPTAPCLALTQCHELHSDRQADSTVTVLCCESLGSFEEVNVSAAEGQTFSFVPVESLKEKTLSSFLEERRPSEGENICVYEHSYCRSDMSKDQLWNKILSLHSKILELDHREECTVARIHALETEIALLKRDGASLIVQTYRITEHIFCPKVDWDIFFLGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BV41</accession>
    <name>A0A673BV41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">WWC family member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">wwc3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029988396.1">
        <property type="nucleotide sequence ID" value="XM_030132536.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A673BV41"/>
    <dbReference type="Ensembl" id="ENSSORT00005046850.1">
        <property type="protein sequence ID" value="ENSSORP00005045695.1"/>
        <property type="gene ID" value="ENSSORG00005020982.1"/>
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    <dbReference type="InParanoid" id="A0A673BV41"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035330">
        <property type="term" value="P:regulation of hippo signaling"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="C2_Kibra"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN WWC3"/>
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    <dbReference type="Pfam" id="PF00397">
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        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF25802">
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="10">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="683"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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    <feature type="region of interest" description="Disordered" evidence="8">
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    </feature>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
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        <location>
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            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
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        <location>
            <begin position="528"/>
            <end position="545"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045695.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1156" mass="130460" checksum="956984F80CAD5D1F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BV51</accession>
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        <recommendedName>
            <fullName evidence="20">Myosin, heavy chain b</fullName>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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        </subcellularLocation>
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        <text evidence="2 13">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
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        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
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        <property type="entry name" value="Myosin_tail"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF44">
        <property type="entry name" value="MYOSIN HEAVY CHAIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9 13" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="33"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="86"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="13">
        <location>
            <begin position="653"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1577"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1675"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1581"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="179"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1941" mass="221977" checksum="5B9088A678100E30" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVZ7</accession>
    <name>A0A673BVZ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">klc3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the normal function of spermatozoa. May play a role in the formation of the mitochondrial sheath formation in the developing spermatid midpiece.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Oligomer composed of two heavy chains and two light chains. Associates with microtubulin in an ATP-dependent manner. Interacts with KIF5C. Interacts with ODF1. Interacts with LRGUK. Interacts with VDAC2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Cytoplasm</location>
            <location evidence="2 18">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BVZ7"/>
    <dbReference type="FunCoup" id="A0A673BVZ7">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046458.1">
        <property type="protein sequence ID" value="ENSSORP00005045309.1"/>
        <property type="gene ID" value="ENSSORG00005020328.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVZ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000003">
        <property type="entry name" value="kinesin light chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF1">
        <property type="entry name" value="KINESIN LIGHT CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 18" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 18" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 18" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="9 17" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="17">
        <location>
            <begin position="290"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="17">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="189"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="555"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="124"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="564"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="585" mass="65154" checksum="8428040D2FDF262C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BW07</accession>
    <name>A0A673BW07_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Platelet-derived growth factor D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pdgfd</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Homodimer; disulfide-linked. Interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030008944.1">
        <property type="nucleotide sequence ID" value="XM_030153084.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005044747.1"/>
        <property type="gene ID" value="ENSSORG00005020590.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432207"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:platelet-derived growth factor receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of cell division"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of cell migration"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of cell population proliferation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUB_dom"/>
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        <property type="entry name" value="Cystine-knot_cytokine"/>
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    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
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    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633:SF4">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-0497">Mitogen</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025349149" description="Platelet-derived growth factor D" evidence="13">
        <location>
            <begin position="25"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="44"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="15">
        <location>
            <begin position="258"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00026039"/>
        </source>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="373" mass="42909" checksum="015BA561202683E2" modified="2020-06-17" version="1">MSGPVVFQMWMLLLLGTTSEVVKAHVSPAQVIRRNVRSDDLYRKEETISVTPLGGIIHSPRYPNAYPRCLLLSWKLLSPPGTRIHLEFDRHFNLEEGENGVCRYDFIEVEDQSETSTIIWGRWCGERAPPTVISKTNALKVTFKSDDYFVAKPGFKIYYTMMHDPPHSQSKTNWEPVTLSSPHELAYDEGGVPEAVTEAPLSLEDLDQTIAIFDTIEDLLKTLNPDTWKQDLDNIYTQPYIQYRSRAYHLMSRHNKVDLNRLYDDVKRYSCTPRNYSVNLREELRVTNAVFFPRCLLVKRCGGNCGCGTDNWNSGCTCQAAKTTLKLHEVLKYAPEANLSQRHRRPKVRWVIDEVFLTHHEKCECACPSQPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BW69</accession>
    <name>A0A673BW69_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115430969</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN1 and CLDN2 homopolymers. Interacts in cis (within the same plasma membrane) with CLDN19. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell junction</location>
            <location evidence="10">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030007044.1">
        <property type="nucleotide sequence ID" value="XM_030151184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BW69"/>
    <dbReference type="Ensembl" id="ENSSORT00005047237.1">
        <property type="protein sequence ID" value="ENSSORP00005046080.1"/>
        <property type="gene ID" value="ENSSORG00005021107.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430969"/>
    <dbReference type="InParanoid" id="A0A673BW69"/>
    <dbReference type="OrthoDB" id="8830244at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003549">
        <property type="entry name" value="Claudin3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01378">
        <property type="entry name" value="CLAUDIN3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0796">Tight junction</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="215" mass="23106" checksum="EF3C289833FEE3CD" modified="2020-06-17" version="1">MSIGLELMGISLCILGWIIAIVACALPMWRVTAFIGSNIVTAQIIWEGLWMTCVVQSTGQMQCKVYDSMLALSQDLQAARALTVISILLAILAVLIAIAGAKCTNCIEDEASKAKVMIISGVFFIVSGVMQLIPVSWSANTIIRDFYNPLLTDAQRRELGAALYIGWAAAALLILGGGLLCCSCPPRETRYNPSRMAYSAPRSAGGPGLERKDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BWV1</accession>
    <name>A0A673BWV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pleckstrin homology-like domain family B member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein LL5-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phldb1b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSSORT00005048567.1">
        <property type="protein sequence ID" value="ENSSORP00005047391.1"/>
        <property type="gene ID" value="ENSSORG00005021672.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BWV1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
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<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="503" mass="56915" checksum="389CFDBB5B1599E7" modified="2020-06-17" version="1">MGCVGSKKEQETLGKGTGNDEQQNRAQTAHYVKDPTTGNSGKNASKMPSNAMSSEGESIAMALYDYEAIHDGDLGFKKGDKLKILEESGEWWRAMLISTGKEGYIPSNYVAKDTLEAEEWFFKGVSRKDAERQLLAPGNKIGSFMIRDSETTKGSYSLSVRDHDAHSADTVKHYKIRTLDNGGFYISPRITFSTLQELVSHYKKQGDGLCQTLTNPCLSPKPEKPWEKDAWEIPRSSLKLEKRLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFMGEANLMKTLQHDKLVRLNAVVTKEEPIYIITEYMEKGSLLDFLKSDEGNRVQLPKLIDFSAQIAEGMAYIEQRNYIHRDLRAANILVNKALVCKIADFGLARIIEDNEYTAREGAKFPIKWTAPEAINYGSFTIKSDVWSFGILLTEIISYGRTPYPGMTNPEVIRSLEKGYRMQRLDSCPTELYEIMLECWKNKPEDRPTFDYLQSVLEDFYTATESQYQQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYC7</accession>
    <name>A0A673BYC7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase-associated protein RTF1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rtf1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSSORT00005047199.1">
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        <property type="gene ID" value="ENSSORG00005021086.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:stem cell population maintenance"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115:SF8">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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            <begin position="317"/>
            <end position="448"/>
        </location>
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            <begin position="1"/>
            <end position="171"/>
        </location>
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            <end position="322"/>
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            <end position="523"/>
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            <begin position="1"/>
            <end position="10"/>
        </location>
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        </location>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="678" mass="77125" checksum="84C3AE1185999F2E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2X8</accession>
    <name>A0A673C2X8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MOK protein kinase</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSSORG00005022548.1"/>
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    </dbReference>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="385" mass="44079" checksum="5D45368FEBDE9D5C" modified="2020-06-17" version="1">LDYHRKCYKLLKKIGEGTFSEVVKTQSLKDGKFYACKTMKQTINSLEQANNLREVQAMKRLSPHANIIQLHELIFDKETGTVSLICELMEMNIYEFIQGRQTPLPDQTVKHYMYQLCKSLEHMHSCGILPQRCEAENILIKQNILKLGDFGSCRSVYSKPPHTEYISTRWYRAPECLLTDGYYSLKMDIWSAGCVFFEIMSLNPLFPGSNELDQVAKIHDVLGTTDQSLLQKFKQSRAMHFNFPPKKGTGISRLIPNCPAPALSLLYQMLAYDPDERITAETALRHTYFREISTFGAPTRLGRQMRGRHTRPNAFEYGTSLITPTELPKLNMVVPGPQISFPVSTMPAFTVTHRGTLPAITSKKCQSRIRRCFHHSQIERKGKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4Z4</accession>
    <name>A0A673C4Z4_9TELE</name>
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        <submittedName>
            <fullName evidence="8">SRY-box transcription factor 21b</fullName>
        </submittedName>
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        <name evidence="8" type="primary">sox21b</name>
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    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C4Z4"/>
    <dbReference type="FunCoup" id="A0A673C4Z4">
        <property type="interactions" value="10"/>
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        <property type="protein sequence ID" value="ENSSORP00005049060.1"/>
        <property type="gene ID" value="ENSSORG00005022302.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022097">
        <property type="entry name" value="SOX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF313">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12336">
        <property type="entry name" value="SOXp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="248" mass="27113" checksum="D1F1E0B7EEB0870C" modified="2020-06-17" version="1">MSKPMDHVKRPMNAFMVWSRAQRRKMAQENPKMHNSEISKRLGAEWKLLTESEKRPFIDEAKRLRAMHMKEHPDYKYRPRRKPKTLMKKDKFSFPVPYNLGEHDALKVNGLSAGALSDSVLGNPDKAAAAAAAAAARVFFNPSMSTNPYSFFDLGSKMTELSPPSFPYASPLGYPPGGATAFTGTGSVGGGTHTHTHSHPSPGNPGYMIPCNCTAWPSAGLQPPLAYILLPGMGKPQLEPYPAYAAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7H7</accession>
    <name>A0A673C7H7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-dependent protein kinase catalytic subunit</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">prkdc</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="FunCoup" id="A0A673C7H7">
        <property type="interactions" value="1164"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052717.1">
        <property type="protein sequence ID" value="ENSSORP00005051486.1"/>
        <property type="gene ID" value="ENSSORG00005022826.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C7H7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004677">
        <property type="term" value="F:DNA-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033152">
        <property type="term" value="P:immunoglobulin V(D)J recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05172">
        <property type="entry name" value="PIKKc_DNA-PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037706">
        <property type="entry name" value="DNA-PK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046804">
        <property type="entry name" value="DNA-PKcs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046803">
        <property type="entry name" value="DNAPKcs_CC1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012582">
        <property type="entry name" value="DNAPKcs_CC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045581">
        <property type="entry name" value="DNAPKcs_CC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF68">
        <property type="entry name" value="DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20500">
        <property type="entry name" value="DNA-PKcs_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20502">
        <property type="entry name" value="DNAPKcs_CC1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08163">
        <property type="entry name" value="DNAPKcs_CC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19704">
        <property type="entry name" value="DNAPKcs_CC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01344">
        <property type="entry name" value="NUC194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="16">
        <location>
            <begin position="2702"/>
            <end position="3368"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="15">
        <location>
            <begin position="3552"/>
            <end position="3853"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="17">
        <location>
            <begin position="3896"/>
            <end position="3928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1921"/>
            <end position="1943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="3928" mass="447306" checksum="62B4A1ED71E9AA98" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7N5</accession>
    <name>A0A673C7N5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rho-related GTP-binding protein Rho6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Rho family GTPase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Rnd1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rnd1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029995423.1">
        <property type="nucleotide sequence ID" value="XM_030139563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C7N5"/>
    <dbReference type="Ensembl" id="ENSSORT00005051218.1">
        <property type="protein sequence ID" value="ENSSORP00005050015.1"/>
        <property type="gene ID" value="ENSSORG00005022694.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422930"/>
    <dbReference type="InParanoid" id="A0A673C7N5"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000569">
        <property type="entry name" value="Rho-related GTP-binding protein Rho6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="232" mass="26029" checksum="29D8E629E2C668B2" modified="2020-06-17" version="1">MKERRLTQPFVARCKLVLVGDVQCGKTAMLQVLAKDCYPETYVPTVFENYTACLELEDQRVELSLWDTSGSPYYDNVRPLCYSDSDAVLLCFDISRPDTVDAALKKWKAEIQDFCPSTRILLIGCKTDLRTDVCTRIELSNQKQAPISHEQGSSLAKQLGAEAYLECSAFTSEKSIHSVFRTAALACMDKLQPANKPSPVRRLSKRLLHLPSKTELLSSTFSKDKSRSCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C8N0</accession>
    <name>A0A673C8N0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Laminin subunit beta-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">lamb2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005053181.1">
        <property type="protein sequence ID" value="ENSSORP00005051941.1"/>
        <property type="gene ID" value="ENSSORG00005023326.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C8N0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043256">
        <property type="term" value="C:laminin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070831">
        <property type="term" value="P:basement membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22299">
        <property type="entry name" value="cc_LAMB2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056558">
        <property type="entry name" value="LAMB1-4_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013015">
        <property type="entry name" value="Laminin_IV_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF36">
        <property type="entry name" value="LAMININ SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23219">
        <property type="entry name" value="LAMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21199">
        <property type="entry name" value="LAMININ_IV_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51116">
        <property type="entry name" value="LAMININ_IVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin N-terminal" evidence="17">
        <location>
            <begin position="12"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="252"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="379"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="439"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type B" evidence="16">
        <location>
            <begin position="530"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="750"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="798"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="844"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="955"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="1007"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="1064"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="15">
        <location>
            <begin position="1112"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1306"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1231"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1418"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1535"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1594"/>
            <end position="1755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1308"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1318"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="474"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="750"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="752"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="771"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="798"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="800"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="863"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1007"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1036"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1064"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1066"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1085"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1112"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1114"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1133"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1767" mass="195173" checksum="D03E0F3A054A9FF1" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CB55</accession>
    <name>A0A673CB55_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clavesin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Retinaldehyde-binding protein 1-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">clvs2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Required for normal morphology of late endosomes and/or lysosomes in neurons. Binds phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029983905.1">
        <property type="nucleotide sequence ID" value="XM_030128045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CB55"/>
    <dbReference type="FunCoup" id="A0A673CB55">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050569.1">
        <property type="protein sequence ID" value="ENSSORP00005049368.1"/>
        <property type="gene ID" value="ENSSORG00005022425.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414755"/>
    <dbReference type="CTD" id="134829"/>
    <dbReference type="InParanoid" id="A0A673CB55"/>
    <dbReference type="OrthoDB" id="7837562at2759"/>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.20:FF:000001">
        <property type="entry name" value="Alpha-tocopherol transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000002">
        <property type="entry name" value="Alpha-tocopherol transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
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        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CLAVESIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRETINALDHBP"/>
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        <property type="entry name" value="CRAL_TRIO_N"/>
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    </dbReference>
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        <property type="entry name" value="SEC14"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="CRAL_TRIO"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="13">
        <location>
            <begin position="96"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="294"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="333" mass="38690" checksum="EDE712EB94B9756C" modified="2020-06-17" version="1">MTHLQAGLSPETLEKAKVELKENPETLHQDIQEVRDMIITRPDIGFLRTDDAFILRFLRARKFNHFEAFRLLAQYFEYRQQNLDMFKNLKATDPGIKQALKDGFPGVLSNLDRYGRKILVLFAANWDQSRYTFVDILRAILLSLESMIEDPELQVNGFILIIDWSNFTFKQASKLTPSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKDKTRKRIFMHGNNLNSLHQLIHPEILPSELGGMMPPYDMGTWARTLLDHAYDEETDYCPESYTLSVQDLERDLEKNLSPKTMKRSQSVVEPGVLKRPDKVKSEEDNMQPLLSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCQ3</accession>
    <name>A0A673CCQ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fucolectin tachylectin-4 pentraxin-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a defensive agent. Recognizes blood group fucosylated oligosaccharides including A, B, H and Lewis B-type antigens. Does not recognize Lewis A antigen and has low affinity for monovalent haptens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fucolectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCQ3"/>
    <dbReference type="Ensembl" id="ENSSORT00005053098.1">
        <property type="protein sequence ID" value="ENSSORP00005051860.1"/>
        <property type="gene ID" value="ENSSORG00005023417.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCQ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042806">
        <property type="term" value="F:fucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010185">
        <property type="term" value="P:regulation of cellular defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001868">
        <property type="term" value="P:regulation of complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051941">
        <property type="entry name" value="BG_Antigen-Binding_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713">
        <property type="entry name" value="FTP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713:SF8">
        <property type="entry name" value="SI:CH211-215K15.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="10">
        <location>
            <begin position="8"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="10">
        <location>
            <begin position="156"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="330" mass="36327" checksum="749648458C3D8868" modified="2020-06-17" version="1">MMNLSTLLQNVAINGMATQSSLNSSFGAPQKAIDGDRNHALTSGSCIQTNEQADPWWMVDLQRTYKIAAVIIGNREGTENNLHGGEIWISSSKDNNSTGRFRCGIITSFPPAATLYFPCRATEGRFVGVFLPGTRRVLSLCEVEVYSVDDAFPTPNVALRGEAAQSSTLSFAAAFKAIDGRRNSFYTKGSCSHTAEGETSPWWRVDLLRTFIITSIKVTNRGDCCAERLDGAEIRIGKSLHNNGNDNPRCATISHIRPGKTLTIHCDNGMMEGRYVNMVIPGNGKTLTLCEVEVYATPKGIAITDWITHSETNHLTFVHSLYFFFFCFNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CD59</accession>
    <name>A0A673CD59_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CD59"/>
    <dbReference type="Ensembl" id="ENSSORT00005054762.1">
        <property type="protein sequence ID" value="ENSSORP00005053506.1"/>
        <property type="gene ID" value="ENSSORG00005024064.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CD59"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="4">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="207" mass="23966" checksum="A74244AC0644BCC2" modified="2020-06-17" version="1">CENATVLNIFYTFHCIHSISTVSYVTVEEGGSISIPCFYESEYTDHVKYLCKGHTWESCTYEVKTNEKSSTKYSVSDNKNQRMVTFIINDLKTKDTNYYWCNMEINEGPDDGKHFHVRVTGKGKSFEFTTVQMGYNVEQCEGRTFVYSGKIDETKVTINRNTPGVITVTMSELRERSSGWYWCTDEDLQMPVHVTIKERPTTSKYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CDE5</accession>
    <name>A0A673CDE5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Leucine rich repeat containing 38b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">lrrc38b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with KCNMA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CDE5"/>
    <dbReference type="Ensembl" id="ENSSORT00005054857.1">
        <property type="protein sequence ID" value="ENSSORP00005053601.1"/>
        <property type="gene ID" value="ENSSORG00005024097.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CDE5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099104">
        <property type="term" value="F:potassium channel activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000015">
        <property type="entry name" value="Leucine rich repeat containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051432">
        <property type="entry name" value="KCNMA1_auxiliary"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473">
        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473:SF4">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025416016" evidence="15">
        <location>
            <begin position="24"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="16">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="17">
        <location>
            <begin position="185"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="288" mass="32201" checksum="81F0DD9C27A3350F" modified="2020-06-17" version="1">MLPCVSWLQLILILLSSVLWTRGENCPVTCLCPDPHTVDCSGRGLTRLPDEIPLDVRRLLLADNWIPRIPSDFLVLYSDLVYLDLRNNTLSHIEPGTLSTSSRLVFLDLGSNNLTEIPKGTFGESRSLIKLRLGNNPYLSLVNEDAFLGLTSLRELELERNALSTLKVGALSQLPSLRVVRLEGNPWVCNCNFANLFTWLMENSHKLPNGMECSLPMDGRRVSLTQLSKDSFRECQATLTLTDLLIIIFSGISVSVVAIMTSFFLASTVHCFQRWSKGTKGDEEESEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CDV1</accession>
    <name>A0A673CDV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115420989</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673CDV1"/>
    <dbReference type="FunCoup" id="A0A673CDV1">
        <property type="interactions" value="1349"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055033.1">
        <property type="protein sequence ID" value="ENSSORP00005053766.1"/>
        <property type="gene ID" value="ENSSORG00005022446.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CDV1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04021">
        <property type="entry name" value="C2_E3_ubiquitin_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000082">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase HACE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF396">
        <property type="entry name" value="NEDD4-LIKE E3 UBIQUITIN-PROTEIN LIGASE WWP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6 9 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="295"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="370"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="409"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="502"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="169"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="211"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10 11">
        <location>
            <position position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="840" mass="95818" checksum="D6F83C3BFD7646A7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CG01</accession>
    <name>A0A673CG01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pwwp2b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030011780.1">
        <property type="nucleotide sequence ID" value="XM_030155920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CG01"/>
    <dbReference type="FunCoup" id="A0A673CG01">
        <property type="interactions" value="1049"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052236.1">
        <property type="protein sequence ID" value="ENSSORP00005051018.1"/>
        <property type="gene ID" value="ENSSORG00005023077.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434167"/>
    <dbReference type="CTD" id="170394"/>
    <dbReference type="InParanoid" id="A0A673CG01"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010369">
        <property type="term" value="C:chromocenter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000036">
        <property type="entry name" value="PWWP domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16112">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, DROSOPHILA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16112:SF22">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PWWP" evidence="4">
        <location>
            <begin position="557"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="115"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="226"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="390"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="660" mass="72744" checksum="6A040A9EEA7760A5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGV8</accession>
    <name>A0A673CGV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STAR protein homodimerisation region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CGV8"/>
    <dbReference type="Ensembl" id="ENSSORT00005054645.1">
        <property type="protein sequence ID" value="ENSSORP00005053390.1"/>
        <property type="gene ID" value="ENSSORG00005024002.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGV8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4010:FF:000001">
        <property type="entry name" value="protein quaking isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032377">
        <property type="entry name" value="STAR_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16544">
        <property type="entry name" value="STAR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAR protein homodimerisation region" evidence="3">
        <location>
            <begin position="10"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="58" mass="6663" checksum="1A8A23E262C039B9" modified="2020-06-17" version="1">MVGDMEGKDRPRPSPDYLMQLMNDKKLMNSLPNFCGIFQHLERLLDEGETRVTNPGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJG6</accession>
    <name>A0A673CJG6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">G-protein coupled receptor family C group 6 member A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115413916</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029982919.1">
        <property type="nucleotide sequence ID" value="XM_030127059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CJG6"/>
    <dbReference type="Ensembl" id="ENSSORT00005054054.1">
        <property type="protein sequence ID" value="ENSSORP00005052802.1"/>
        <property type="gene ID" value="ENSSORG00005023797.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413916"/>
    <dbReference type="InParanoid" id="A0A673CJG6"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF422">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 3 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025349875" evidence="12">
        <location>
            <begin position="20"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="581"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="615"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="659"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="735"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="763"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="547"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="821" mass="92101" checksum="A3CE711141FFA54A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CK45</accession>
    <name>A0A673CK45_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ELAV-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">elavl1a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the RRM elav family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CK45"/>
    <dbReference type="Ensembl" id="ENSSORT00005053690.1">
        <property type="protein sequence ID" value="ENSSORP00005052443.1"/>
        <property type="gene ID" value="ENSSORG00005023622.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CK45"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12773">
        <property type="entry name" value="RRM2_HuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12653">
        <property type="entry name" value="RRM3_HuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000006">
        <property type="entry name" value="ELAV-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000005">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000383">
        <property type="entry name" value="Sex lethal, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006548">
        <property type="entry name" value="ELAD_HU_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034996">
        <property type="entry name" value="HuR_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01661">
        <property type="entry name" value="ELAV_HUD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025545245" description="ELAV-like protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="47"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="141"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="279"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="361" mass="39763" checksum="9E755D74A704F8AD" modified="2020-06-17" version="1">MAVVLFILDVLICCCFSAHLADQQEVFDMSNGYEDHMGGDEGKDAKTNLIVNYLPQSMTQDELRSLFSSIGEVESAKLIRDKVAAQPYLTVDSHSLGYGFVNYLNPSDADRAINTLNGLRLQSKTIKVSYARPSSDTIKDANLYISGLPKSMTQKDVEDMFSRYGRIINSRVLVDQGTGASRGVAFIRFDKRAEAEEAVKNLNGHKPPGASEPITVKFAANPNQVKNTQLISQLYHNQSRRFGGPLHHQAQRFRFSPMGVDHMGGMGGVGVPGNSTSGWCIFIYNLGQDADESVLWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTSKGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKE9</accession>
    <name>A0A673CKE9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">vesicle-fusing ATPase</fullName>
            <ecNumber evidence="3">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">vps4b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="3"/>
    <dbReference type="RefSeq" id="XP_030015977.1">
        <property type="nucleotide sequence ID" value="XM_030160117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CKE9"/>
    <dbReference type="Ensembl" id="ENSSORT00005057364.1">
        <property type="protein sequence ID" value="ENSSORP00005056071.1"/>
        <property type="gene ID" value="ENSSORG00005024977.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437040"/>
    <dbReference type="CTD" id="9525"/>
    <dbReference type="InParanoid" id="A0A673CKE9"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000004">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF72">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIT" evidence="18">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="MIT" evidence="18">
        <location>
            <begin position="76"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="230"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="145"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="508" mass="57183" checksum="96DA55D5F562EA3B" modified="2020-06-17" version="1">MEPTNLQKAIAIAQKASQEDQAENYEEAIRSYQHAVKYFLHILKREPQGKDGNQKIRDSCKQYLDRVEVLQEFLANKEKAIDLATKAAQEDKAQNYEEALRLYQAAVQYFLHVVKYEAQSAKAKESIRAKCTEYLDRAEKLKEYLKKKEKAPPAKPVKESQSDDRGNESDEGDDPEKKKFQNQLSGAIVMEKPNIKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRVPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFSLAREHKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNEGVLVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARSFMFKLHLGSTPNCLTESDFVALGKKTEGYSGADISIIVRDALMQPVRKVQSATHFKRVRGPSRTDPNIIVDDLLTPCSPGDPQAMEMSWMDVPGEKLMEPVVSMSDMLRSQAHTKPTVNEQDLEKLKKFTEDFGQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKP5</accession>
    <name>A0A673CKP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase 47</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deubiquitinating enzyme 47</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ubiquitin thioesterase 47</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-specific-processing protease 47</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">usp47</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="Ensembl" id="ENSSORT00005055126.1">
        <property type="protein sequence ID" value="ENSSORP00005053859.1"/>
        <property type="gene ID" value="ENSSORG00005023548.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKP5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045578">
        <property type="entry name" value="USP47_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF702">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25985">
        <property type="entry name" value="Ubiquitin_USP47_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19718">
        <property type="entry name" value="USP47_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="175"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="105"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="408"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="890"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="941"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="890"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="917"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="954"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="973"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1027"/>
            <end position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1383" mass="156474" checksum="6758BB39D799FAED" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CL29</accession>
    <name>A0A673CL29_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">paip2b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAIP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030017755.1">
        <property type="nucleotide sequence ID" value="XM_030161895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CL29"/>
    <dbReference type="Ensembl" id="ENSSORT00005056201.1">
        <property type="protein sequence ID" value="ENSSORP00005054925.1"/>
        <property type="gene ID" value="ENSSORG00005024568.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438326"/>
    <dbReference type="CTD" id="400961"/>
    <dbReference type="InParanoid" id="A0A673CL29"/>
    <dbReference type="OrthoDB" id="5985142at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040396">
        <property type="entry name" value="PAIP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13154">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13154:SF5">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="2" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="137" mass="15434" checksum="8AF9716031B4E603" modified="2020-06-17" version="1">MPEPAEMSGPEVAKTPGGGAPGKEGKEPVANGHAGEGNDANPFAEYMWMENEEEYNRQVEEELLEQEFLERCFQEMLEEEDQDWFIPSRDLNNQGVGQLQQQLNGLSVSDHHSNLEEVARKSVLNPEAKEFIPGKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLZ5</accession>
    <name>A0A673CLZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">DnaJ homolog subfamily A member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">dnaja</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="23">Identified in a complex with HSPA1B and BAX. Interacts with RNF207.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CLZ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005057966.1">
        <property type="protein sequence ID" value="ENSSORP00005056666.1"/>
        <property type="gene ID" value="ENSSORG00005025196.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLZ5"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000068">
        <property type="entry name" value="Chaperone protein dnaJ 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000014">
        <property type="entry name" value="dnaJ homolog subfamily A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000005">
        <property type="entry name" value="DnaJ homolog subfamily A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000003">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="18" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0492">Microsome</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-0636">Prenylation</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 24" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 24" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="26">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="27">
        <location>
            <begin position="120"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="24">
        <location>
            <begin position="120"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="356"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00040977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00046752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="394" mass="44565" checksum="7D62C2DFE78DAE38" modified="2020-06-17" version="1">MVRETGYYDLLGVSPKASADEIKKAYRKLALKYHPDKNPNEGDKFKLISQAYEVLSDPKRRDLYDQGGEQAIKEGGTGGGSSPMDIFNMFFGGGGRMQRERRGKNVVHQLEVTLEEIYNGSTRKLGLQKNVICEKCDGYGGKKGTLDKCSTCKGRGVQIKVQQIGPGMIQQIQSMCSDCQGQGERFNSKDRCKNCNGHKVERKRKILEVHIDKGMRDGQKITFSGEGDQEPGLEPGDVVIVLDQKEHAVFQRQGDNLVMKMDIKLVEALCGFKKTIQTLDNRTLVISSLPGEVIKHNDVRCVQNEGMPIYKDPYEKGQLIIQFQVEFPEKDWLPEHLMYQLERLLPPREDVMLTDEMEEVDLVEVDERSQQKKYGEVYDEDEDSPSGGVQCQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CNR3</accession>
    <name>A0A673CNR3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ETS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CNR3"/>
    <dbReference type="Ensembl" id="ENSSORT00005056255.1">
        <property type="protein sequence ID" value="ENSSORP00005054979.1"/>
        <property type="gene ID" value="ENSSORG00005024586.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CNR3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000059">
        <property type="entry name" value="ETS translocation variant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF165">
        <property type="entry name" value="ETS DOMAIN-CONTAINING TRANSCRIPTION FACTOR ERF-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="8">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="139" mass="16687" checksum="E606F40F3E9C4287" modified="2020-06-17" version="1">SKSPGFAFPDWAYKPESSPGSRQIQLWHFILELLQKEEYQGVIAWQGDYGEFVIKDPDEVARLWGIRKCKPHMNYDKLSRALRYYYNKRILHKTKGKRFTYKFNFSKVVLVNYPILDMGQNYMSFCLICIKKRKKKYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSI8</accession>
    <name>A0A673CSI8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DnaJ homolog subfamily B member 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dnajb12a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CSI8"/>
    <dbReference type="FunCoup" id="A0A673CSI8">
        <property type="interactions" value="1734"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056790.1">
        <property type="protein sequence ID" value="ENSSORP00005055507.1"/>
        <property type="gene ID" value="ENSSORG00005024775.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSI8"/>
    <dbReference type="OrthoDB" id="442087at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000004">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF8">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="110"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="42"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="370" mass="42203" checksum="D1035850E8FFAA5F" modified="2020-06-17" version="1">MDSNKDEAERCIKIALNAISNNQSDKARKFLEKAQRLFPTDQAKNLLESLGQNGKPPDTNGSPMNGDGPTMRHRGHAEESDTSGQGATDSAKSYTAEQLEAVRKIKSCKDYYQILGVEKSASEDDLKKAYRKLALKFHPDKNHAPGATEAFKAIGNAYAVLSNAEKRRQYDQYGEERTHTTRQRHHREFEADISPEDLFNMFFGGGFPSSNVHVYRNGRMHFAHQNRQERREQQRDGGLALFVQLMPILILIIVSALSQMMVTQPPYSLSYRPSAGHIHKRHTANLKVSYYVGDRFNEEYSGNNLKNVERSVEEDYISNLRNNCWKEKQQKEGLLYRARYFGDSELYQRAQRMGTPSCSRLSEIQVILDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CXP1</accession>
    <name>A0A673CXP1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SET binding factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sbf2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029986671.1">
        <property type="nucleotide sequence ID" value="XM_030130811.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A673CXP1">
        <property type="interactions" value="1335"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058891.1">
        <property type="protein sequence ID" value="ENSSORP00005057572.1"/>
        <property type="gene ID" value="ENSSORG00005024405.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416938"/>
    <dbReference type="CTD" id="81846"/>
    <dbReference type="InParanoid" id="A0A673CXP1"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13339">
        <property type="entry name" value="PH-GRAM_MTMR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01235">
        <property type="entry name" value="PH_Sbf1_hMTMR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000093">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.200:FF:000004">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000006">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037823">
        <property type="entry name" value="MTMR13_PH-GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022096">
        <property type="entry name" value="SBF1/SBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF4">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12335">
        <property type="entry name" value="SBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="7">
        <location>
            <begin position="6"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Myotubularin phosphatase" evidence="8">
        <location>
            <begin position="1108"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="1792"/>
            <end position="1896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1053"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1068"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1080"/>
            <end position="1091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1898" mass="212954" checksum="21572145080BB413" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CZ57</accession>
    <name>A0A673CZ57_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Neogenin 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">neo1a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily. DCC family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005059580.1">
        <property type="protein sequence ID" value="ENSSORP00005058257.1"/>
        <property type="gene ID" value="ENSSORG00005025626.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CZ57"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd05722">
        <property type="entry name" value="IgI_1_Neogenin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05723">
        <property type="entry name" value="IgI_4_Neogenin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000273">
        <property type="entry name" value="contactin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000101">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000106">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000133">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000189">
        <property type="entry name" value="Neogenin isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000216">
        <property type="entry name" value="neogenin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000187">
        <property type="entry name" value="neogenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000551">
        <property type="entry name" value="Protogenin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010560">
        <property type="entry name" value="Neogenin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF14">
        <property type="entry name" value="NEOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1083"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="25"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="127"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
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            <begin position="216"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="312"/>
            <end position="397"/>
        </location>
    </feature>
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        <location>
            <begin position="427"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="526"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="621"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="725"/>
            <end position="815"/>
        </location>
    </feature>
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        <location>
            <begin position="840"/>
            <end position="936"/>
        </location>
    </feature>
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        <location>
            <begin position="941"/>
            <end position="1038"/>
        </location>
    </feature>
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        <location>
            <begin position="1112"/>
            <end position="1206"/>
        </location>
    </feature>
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        <location>
            <begin position="1281"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1362"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1133"/>
            <end position="1158"/>
        </location>
    </feature>
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        <location>
            <begin position="1287"/>
            <end position="1308"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00009588"/>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1455" mass="159583" checksum="A4A27FCF1B2930FF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y241</accession>
    <name>A0A672Y241_9TELE</name>
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        <submittedName>
            <fullName evidence="11">Arf-GAP with dual PH domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">zgc:92360</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y241"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR037849">
        <property type="entry name" value="PH1_ADAP"/>
    </dbReference>
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        <property type="entry name" value="PH2_ADAP"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021:SF3">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PH_DOMAIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="10">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="130"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="253"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="381" mass="44157" checksum="213D6F51F0049CEF" modified="2020-06-17" version="1">MKFVLHIHSLKRNSRSAHAHRSGCLVEVYPTWGSCTLGVFICLECSGIHRNIPDISKVKSLSLSHWEDHELKFMAENGNEAMKGKYESAVPVYYYKPTHRDCQVLREQWIRAKYERKEFCEAVNSFRYEEGTRDGVLMKRGRDNGQFLSRRFVLSEREGTLKYFTKYDAKEPKAVIKVDTINATFQPEKIGNPNGLQITYLKDYSTRNIFVYHENGKEIVDWFNSIRAVQLHYLKVAFPGATDAELVPKLTRNFLKEGHMEKTGPKQTEGFKKRWFTLDHRRLMYFKDPLDAFAKGEVFLGNRDHGYSASAGLPPGTHCNGAWQHGITIVTPDRCFLFTCETESDQQDWLKHFNDVMSAQMSPQEFTSEDSECRLWQLVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6W2</accession>
    <name>A0A672Y6W2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19 23">7-dehydrocholesterol reductase</fullName>
            <shortName evidence="23">7-DHC</shortName>
            <ecNumber evidence="18 23">1.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20 23">Sterol Delta(7)-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">dhcr7</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes the last step of the cholesterol synthesis pathway, which transforms cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol,7-DHC) into cholesterol by reducing the C7-C8 double bond of its sterol core. Can also metabolize cholesta-5,7,24-trien-3beta-ol (7-dehydrodemosterol, 7-DHD) to desmosterol, which is then metabolized by the Delta(24)-sterol reductase (DHCR24) to cholesterol. Modulates ferroptosis (a form of regulated cell death driven by iron-dependent lipid peroxidation) through the metabolic breakdown of the anti-ferroptotic metabolites 7-DHC and 7-DHD which, when accumulated, divert the propagation of peroxyl radical-mediated damage from phospholipid components to its sterol core, protecting plasma and mitochondrial membranes from phospholipid autoxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>7-dehydrodesmosterol + NADPH + H(+) = desmosterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17737"/>
            <dbReference type="ChEBI" id="CHEBI:27910"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:46741"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>cholesterol + NADP(+) = 7-dehydrocholesterol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23984"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:23986"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 23">Steroid biosynthesis; cholesterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 23">Endoplasmic reticulum membrane</location>
            <topology evidence="1 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.21" evidence="18 23"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y6W2"/>
    <dbReference type="FunCoup" id="A0A672Y6W2">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002407.1">
        <property type="protein sequence ID" value="ENSSORP00005002347.1"/>
        <property type="gene ID" value="ENSSORG00005001407.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y6W2"/>
    <dbReference type="UniPathway" id="UPA00063"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047598">
        <property type="term" value="F:7-dehydrocholesterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000004">
        <property type="entry name" value="7-dehydrocholesterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF38">
        <property type="entry name" value="7-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 23" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="5 23" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8 23" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 23" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 23" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="12 23" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 23" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="17 23" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 23" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="16 23" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="478" mass="55405" checksum="05D3E1063D3DE0C0" modified="2020-06-17" version="1">MNGEMSMEATRRRTQHSANGKSSEEVKQPAQWGRAWEVDWFSLMGVITLLCFAPFIVFYFVMACDQYQCSISQPMLELYSGETTLLSIWARSPSFTWSAAKIYAIWVAFQVFLYMCVPDVTHKFIPGYVGGVQDGARTPAGLINKYEINGLQSWLITHALWVANAHYFHWFSPTIIFDNWIPLMWCTNILGYAVSTFAFIKAYLFPTNSEDCKFTGNVFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSYMAKQQELYGHVTNSMILVNILQAMYVLDFFWNEAWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTAHALAVLLLGLIGYYIFRSTNHQKDLFRRTEGSCSIWGRKPTYIECSYSSADGEIHRSKLLTSGFWGVARHLNYTGDLMGSLSYCAACGFGHLLPYFYIVYMTILLVHRCVRDEHRCSSKYGKDWKRYTNAVPYRLIPAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9N5</accession>
    <name>A0A672Y9N5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAGA-associated factor 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Coiled-coil domain-containing protein 101</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">SAGA complex-associated factor 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">sgf29</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y9N5"/>
    <dbReference type="FunCoup" id="A0A672Y9N5">
        <property type="interactions" value="517"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000620.1">
        <property type="protein sequence ID" value="ENSSORP00005000594.1"/>
        <property type="gene ID" value="ENSSORG00005000400.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y9N5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20393">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20394">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000026">
        <property type="entry name" value="SAGA-associated factor 29 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000029">
        <property type="entry name" value="SAGA-associated factor 29 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037802">
        <property type="entry name" value="SGF29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010750">
        <property type="entry name" value="SGF29_tudor-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047288">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047287">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539:SF0">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07039">
        <property type="entry name" value="SGF29_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51518">
        <property type="entry name" value="SGF29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SGF29 C-terminal" evidence="11">
        <location>
            <begin position="149"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="307" mass="35037" checksum="41099DCD02867FEE" modified="2020-06-17" version="1">MSADTKIAELLTELHQLIKQTQEERSRSEHNLLNIQKTHERMQTENKTSPYYRTKLRGLYTTAKADAEAECSILRHALDKIAEIKSLLEERRIAAKMAGVYSDSDPPRKTMRRGVLMTLLQQSAMTLPLWIGKPGESPPPLCGATPANSDYVAKQGDKVAARVKAMDGDEQWILAEVVSYNHSTNKYEVDDIDEEGKERHTLSRRRIIPLPQWKANPETDPEALFSKDQLVLALYPQTTCFYRALIHTPPHRPQDDYSVLFEDTSYADGYSPPLNVAQSIRGRLQREQEEMTRRRRTKRRSGSRGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBI2</accession>
    <name>A0A672YBI2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YBI2"/>
    <dbReference type="Ensembl" id="ENSSORT00005002111.1">
        <property type="protein sequence ID" value="ENSSORP00005002054.1"/>
        <property type="gene ID" value="ENSSORG00005001265.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YBI2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="43860" checksum="BC8355E542697C9B" modified="2020-06-17" version="1">MRVESLASDVFKTLLDKLREANVMSLAVDESTDNSDVAQLCLYVRFFDGECFPEDLLGLIPLEGHTTGEILFTKIHVGGGAPSMMGRDQWLATRMAAAAPQMRLPRCLIHQSLLCAKLSGELKETMDSLMAIINFIRCTSSLQHRLFRKLLTDMFAEYKDLLIHNDIRWLSKGNALKCFCELREEILFNATVCFLSDVFHHLNQLNMELQGRDKTVAELVEKLHAFQRKLSLFSADLCLMISASPVKWLRFVKDPFCVNVEADFVLKGKELVPSLDEGLLQLELIDIQSSDDLRQSLQQAGFEKFWTHVVSREKFPHSRGLVLFLLTMFGSTYTCESSFSHMNAIKTHNCISLTDQHLQHCSRIALTTYTPDFTALAKSKKCHFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCP5</accession>
    <name>A0A672YCP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YCP5"/>
    <dbReference type="Ensembl" id="ENSSORT00005000552.1">
        <property type="protein sequence ID" value="ENSSORP00005000526.1"/>
        <property type="gene ID" value="ENSSORG00005000360.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCP5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000013">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF15">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="38"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="138"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="4"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="288" mass="30902" checksum="EC02B14F34D10A1A" modified="2020-06-17" version="1">MFQCEGHESALLDCGSSGAQTCSSGTAVDLTCTDPNDVRLVGGASRCSGTVQIKHHGEWRDLEYDFNYYDTWTLKAADVICRRLDCGSAVSGRRSKPFGPKWFISPICLLSTPALTDCVRKHSVFTNPALFLTCSDSVRLVHGSGLCSGRLEVRSNQSWSSVCEEDLDLNDTQVVCRELGCGAPGLLQGRLYGEGEAPVWTSELHCEGHESAVLDCRRSSSAGKTCSPGTAAGLTCTGIKGGGGFIRQCVPLTHVCVSAQIQVVSGWWDSQAAVLVLWRSNTRDTGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YD15</accession>
    <name>A0A672YD15_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger matrin-type protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">zmat1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YD15"/>
    <dbReference type="Ensembl" id="ENSSORT00005001731.1">
        <property type="protein sequence ID" value="ENSSORP00005001680.1"/>
        <property type="gene ID" value="ENSSORG00005001052.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YD15"/>
    <dbReference type="OrthoDB" id="1925236at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46742">
        <property type="entry name" value="LYSINE-RICH COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46742:SF2">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="580"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="610" mass="69554" checksum="222428D01BFDEB8C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDE0</accession>
    <name>A0A672YDE0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Breast cancer anti-estrogen resistance protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">bcar3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YDE0"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="196"/>
            <end position="295"/>
        </location>
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            <begin position="706"/>
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            <begin position="629"/>
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        </location>
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        <location>
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        </location>
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        <location>
            <begin position="482"/>
            <end position="499"/>
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        <location>
            <begin position="537"/>
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    </feature>
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        <location>
            <begin position="583"/>
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    </feature>
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        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003154.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="980" mass="108227" checksum="575CC26A12F85F20" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF10</accession>
    <name>A0A672YF10_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">ornithine decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115436574</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-ornithine + H(+) = putrescine + CO2</text>
            <dbReference type="Rhea" id="RHEA:22964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="4.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ornithine decarboxylase"/>
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        <property type="entry name" value="Alanine racemase"/>
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        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="De-COase2_N"/>
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        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
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        <property type="entry name" value="PLP-binding_barrel"/>
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        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
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    </dbReference>
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        <property type="entry name" value="ORNITHINE DECARBOXYLASE 1-RELATED"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ODADCRBXLASE"/>
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        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
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        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="PLP-binding barrel"/>
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    </dbReference>
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        <property type="entry name" value="ODR_DC_2_1"/>
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    </dbReference>
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        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="3 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="12">
        <location>
            <begin position="49"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="435" mass="48663" checksum="3B03463C393A9464" modified="2020-06-17" version="1">MDLCDIEILDTTKTISDFINDKIKERHSVVSIPDIIYLHEVSFHVANLDDLHQKHIRWLRTLPRVKPFYAVKCNSTPAVIRMLSTLGTGFDCASKGEIELVLSLGVTPEKIIFAHTTKPQSHIKYASTRGVDMMTFDCEEELLKISACHTRAKLVLRIAVDDSKSAIKLSQKFGTKPSVVKKLLERCKELDLEVIGVSFHVGSGCTDSLAYRQAIEDARHVFDTAVLLGFQMRLLDIGGGFPGKEGFQIQFDEFSKVINEALDKFFPADSGVQVIAEPGRYYVDSAFTLAVNVFAKKVVMDDVTQIRVMMYYISDGVFGSLNCLINDPAHTNVAPYLHRKRYRSIIWGPTCDSLDRVTANYWMPEVQVGDWLLIDNMGAYSVSISSDFGFFDKADIYPVLSADVVEAQAPCALSSHIYLSLPYLTLWVRGGAVHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF44</accession>
    <name>A0A672YF44_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10350" checksum="9CB28FDE63682EEC" modified="2020-06-17" version="1">MAQGSQKFKAQRPGANKKHQSNKQKGPKKGGRIIAPKKAQVIEQQKLKKGLEVAIRNKIEQEVTQKASSSLHKPLSVVKGAEGKGGQGPSRPGSSSK</sequence>
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    <accession>A0A672YI43</accession>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    </feature>
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        </source>
    </evidence>
    <sequence length="541" mass="58477" checksum="13E0400BE49A7936" modified="2020-06-17" version="1"> </sequence>
</entry>
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    <accession>A0A672YI73</accession>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5025665903" description="PAT complex subunit CCDC47" evidence="9">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="406"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="435"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="488" mass="56284" checksum="0367E6037DE65B34" modified="2020-06-17" version="1">MRSLRLFLIPTLLLLLALPVSRGRYNDDFDDGEDLADDNDFAEFEDMNDDPVAETETMPPPRVSFPSQVDDEEDEDEATVELEDGQDGFEDSETQDQDIFSKYDQEEFEGIGDMEKTIHSMKDPLIIHTVPAHLQNNWESYYMEILMVTGLLAYIMNYIIGKNKNSRLAHAWFNSHRELLESNFALVGDDGTSKEAVSTGKLNQENEHIYNLWCSGRVCCEGMLIQLKFLKRQDLLNVLARMMRPACDQVQIKVTLNDEDMDTFVFAVGTKKAMARLQKEMQDLSEFCGDKPKSGAKYGLPDSLAILSEMGEVTDGVMDNKMVHYITNHADKIESIHFSDQFSGPKIMQEEGQPLKLPETKKTLLFTFNVPGMGNTSPKDMDTLLPLMNMVIYSIDKVKKLRLNREGKQKADRNRARVEENFLKQTHAQRQEAAQTRREEKKRAEKERIMNEEDPERQRRLEEAAQRREQKKIEKKQMKMKQIKVKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YI86</accession>
    <name>A0A672YI86_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAM184A/B N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YI86"/>
    <dbReference type="Ensembl" id="ENSSORT00005004402.1">
        <property type="protein sequence ID" value="ENSSORP00005004276.1"/>
        <property type="gene ID" value="ENSSORG00005002588.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YI86"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039478">
        <property type="entry name" value="FAM184A/B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870:SF7">
        <property type="entry name" value="PROTEIN FAM184A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870">
        <property type="entry name" value="PROTEIN TAG-278-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15665">
        <property type="entry name" value="FAM184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein FAM184A/B N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="8"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="333" mass="38760" checksum="B9AB5ACEB9C79211" modified="2020-06-17" version="1">VTSVIYALNTKNDEHEAAIATLKEAHEEEVQQILSETREKILQYKSKISDEMDLKRRIQSLEESMELHERMKRQALAEFESYRQRVEDMQLCTDAQHTQRVVSMSREVEEMRRSFEEKLRTFSQAQSPFEQEKKVALEELKAQHRQEIQELLRRPPEPERQLQQRPGETGTVDSLTERVEELKQDKKRLVEEYEAKLSKVRSIIKMGYVWDQTRCRTSDTNVAFQSRAAAEFQTQEAALQKTLGKLRSELARVSRGPENREKSHKLQSSLNTAEGTIKDLTKQLDEVTQNSEIVEIRQKEAECELEAARDRVQQQATEILLKASESTAPVLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJL9</accession>
    <name>A0A672YJL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lon N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJL9"/>
    <dbReference type="Ensembl" id="ENSSORT00005004902.1">
        <property type="protein sequence ID" value="ENSSORP00005004763.1"/>
        <property type="gene ID" value="ENSSORG00005002885.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJL9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="1">
        <location>
            <begin position="29"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="158" mass="17444" checksum="4763FC512E7F7731" modified="2025-10-08" version="2">MPGSTVRFSVGLSSEHAPGEEPPCVCLFLFRVGTAGIAVQVVGSNWPKPHYTLLITGLCRFSVTSLLKERPFVLAEVQHMRCPTEDGELGELSQKFYQAALQLLGMLDMSVPVVAKFRRLLDSLPRETLPDVVASMIRTSNKEKLQVGDFGFDAKNMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKK5</accession>
    <name>A0A672YKK5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dual specificity mitogen-activated protein kinase kinase 2</fullName>
            <ecNumber evidence="9">2.7.12.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ERK activator kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">MAPK/ERK kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">map2k2a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A672YKK5"/>
    <dbReference type="FunCoup" id="A0A672YKK5">
        <property type="interactions" value="1718"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005296.1">
        <property type="protein sequence ID" value="ENSSORP00005005145.1"/>
        <property type="gene ID" value="ENSSORG00005003065.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YKK5"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000641">
        <property type="term" value="P:regulation of early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090170">
        <property type="term" value="P:regulation of Golgi inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032872">
        <property type="term" value="P:regulation of stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06649">
        <property type="entry name" value="PKc_MEK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000115">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000100">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050915">
        <property type="entry name" value="MAP_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448:SF3">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="71"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="399" mass="44289" checksum="368194F8D05D2B24" modified="2020-06-17" version="1">MAPKRRPVPLTITPIGEGQATSNTIDAASEANLEALQKKLGELDLDEQQRKRLEAFLTQKAQVGELKDEDFDPICELGAGNGGVVNKVRHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKEARRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDVWSMGLSLVELAIGRYPIPPPDAKELEAIFGRAVMDGAEGEPHTNMQRPRPPGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLPHGVFTNDFQDFVTKCLIKNPAERADLKMLMSHTFIKRSEVEEVDFAGWLCKTMGLNQPSTPTRGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKR7</accession>
    <name>A0A672YKR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Solute carrier family 25 member 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">slc25a17</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YKR7"/>
    <dbReference type="Ensembl" id="ENSSORT00005005279.1">
        <property type="protein sequence ID" value="ENSSORP00005005128.1"/>
        <property type="gene ID" value="ENSSORG00005003079.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YKR7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080122">
        <property type="term" value="F:AMP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005347">
        <property type="term" value="F:ATP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015230">
        <property type="term" value="F:FAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044610">
        <property type="term" value="F:FMN transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000047">
        <property type="entry name" value="peroxisomal membrane protein PMP34 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF4">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="9"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="101"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="203"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="34755" checksum="8CDBFDD54590FA6B" modified="2020-06-17" version="1">TMSSEVFSYESLVHAVSGAMGSVTAMTVFFPLDTARLRLQVDDNRKAKSTPAILAEIIKEEGLLAPYRGWFPVICSLCCSNFVYFYCFHSLKAGWLKGQKSTPGSDLIIGIAAGVVNVLVTTPLWVVNTRLKLQGSKFRNADIRSTNYSGILDAFVQIIRDEGVGALWNGTFPSLLLVMNPAIQFMIYEGLKRQLRRGVPRELSSLEVFIIGAVAKAVATTVTYPLQTIQSILRFGQFNESTDKSKLLSSLRTIKCLLVNRARRFGMLGLFKGLEAKLLQTVLTAALMFLLYEKIASCTFRVMGLKNSHYKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YN59</accession>
    <name>A0A672YN59_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Abl-interactor 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">abi2b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YN59"/>
    <dbReference type="Ensembl" id="ENSSORT00005006223.1">
        <property type="protein sequence ID" value="ENSSORP00005005973.1"/>
        <property type="gene ID" value="ENSSORG00005003551.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YN59"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11972">
        <property type="entry name" value="SH3_Abi2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000002">
        <property type="entry name" value="abl interactor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028457">
        <property type="entry name" value="ABI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035726">
        <property type="entry name" value="Abi2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012849">
        <property type="entry name" value="Abl-interactor_HHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460:SF26">
        <property type="entry name" value="ABL INTERACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460">
        <property type="entry name" value="ABL INTERACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07815">
        <property type="entry name" value="Abi_HHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="45"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="478"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="149"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="346"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="420"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="214"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="243"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="540" mass="59013" checksum="90ACDFAB4E769D8F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPA2</accession>
    <name>A0A672YPA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPA2"/>
    <dbReference type="Ensembl" id="ENSSORT00005006688.1">
        <property type="protein sequence ID" value="ENSSORP00005006431.1"/>
        <property type="gene ID" value="ENSSORG00005003788.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPA2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:signaling receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:positive regulation of JNK cascade"/>
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        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047526">
        <property type="entry name" value="TNR19/27/EDAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120">
        <property type="entry name" value="TNFR-CYS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120:SF10">
        <property type="entry name" value="TNFR-CYS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025687133" description="TNFR-Cys domain-containing protein" evidence="10">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="11">
        <location>
            <begin position="51"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="9">
        <location>
            <begin position="51"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="97" mass="10370" checksum="7675970553B79BFE" modified="2020-06-17" version="1">IQPWTVCQLRLYPVPLLSLPLLNGSQLLIQYTAHTLQDCGFGDGGEGICIMCEQGTFSSDTSVAPCTRCTQCNLLNRLQKTGCSSTTDALCGQCLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQ14</accession>
    <name>A0A672YQ14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">vps16</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Late endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="3">Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the VPS16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YQ14"/>
    <dbReference type="Ensembl" id="ENSSORT00005006946.1">
        <property type="protein sequence ID" value="ENSSORP00005006686.1"/>
        <property type="gene ID" value="ENSSORG00005002138.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YQ14"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025387541" description="Vacuolar protein sorting-associated protein 16 homolog" evidence="4">
        <location>
            <begin position="20"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 N-terminal" evidence="6">
        <location>
            <begin position="46"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="5">
        <location>
            <begin position="544"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="5">
        <location>
            <begin position="681"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="850" mass="95208" checksum="EFD4142E4F6980C9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YR35</accession>
    <name>A0A672YR35_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Ceramide synthase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cers2a</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sphinganine + octadecanoyl-CoA = N-(octadecanoyl)-sphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:67033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36548"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YR35"/>
    <dbReference type="Ensembl" id="ENSSORT00005007239.1">
        <property type="protein sequence ID" value="ENSSORP00005006977.1"/>
        <property type="gene ID" value="ENSSORG00005004033.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YR35"/>
    <dbReference type="OrthoDB" id="537032at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000020">
        <property type="entry name" value="Ceramide synthase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF7">
        <property type="entry name" value="CERAMIDE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="17">
        <location>
            <begin position="84"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="18">
        <location>
            <begin position="131"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="86"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="339"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="382" mass="44979" checksum="EF51A1A8CA8D16DB" modified="2020-06-17" version="1">MLSQLREQIWADWIWFPEGHGWADLTDHDGKVFPKTADLWATIPIALCFLVIRQIFERTVAIPLASLLGVRDKKRVFAAPNPTLESYFCSTSKHPTQSSIESLSKHTGCSVRQVQRWFRRRRNQDRPSKVKKFREASWRFTFYLLAFFAGLAVLIDKPWFYDMKLMWEDFPKMPLVPSQYWYYMIELGFYISLLFSVASDVKRKDFKEQIVHHVATISLIGFSWLVNYIRAGTLIMLAHDASDYLMESAKMFNYAGWRKTCNFIFTVFAAVFIVTRLVILPFWIIHTTWVYPLTLYPPFFGFYFFNGLLLVLQCLHIFWAALIIRMVIKFLPGNDIVEDERSDKEETDSEDEDDDHEWREKSKNGHVQNGHAPLNNNHRKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRQ3</accession>
    <name>A0A672YRQ3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Basic leucine zipper transcription factor, ATF-like 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YRQ3"/>
    <dbReference type="Ensembl" id="ENSSORT00005007538.1">
        <property type="protein sequence ID" value="ENSSORP00005007263.1"/>
        <property type="gene ID" value="ENSSORG00005004163.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YRQ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF13">
        <property type="entry name" value="BASIC LEUCINE ZIPPER TRANSCRIPTIONAL FACTOR ATF-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="19"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="99" mass="11621" checksum="2CDFC9DDDF0C86BA" modified="2020-06-17" version="1">MFQLTLNTVTSWTKGSDDDGRRMKRREKNRVAAQKSRKRQTQRADLLHQACEVLEERNRNLRREVDSLSEEQYLLTEALRTHEPLCPIMHLGVASSDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YV28</accession>
    <name>A0A672YV28_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="8 11">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115422219</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="8 11"/>
    <dbReference type="AlphaFoldDB" id="A0A672YV28"/>
    <dbReference type="FunCoup" id="A0A672YV28">
        <property type="interactions" value="2069"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008626.1">
        <property type="protein sequence ID" value="ENSSORP00005008332.1"/>
        <property type="gene ID" value="ENSSORG00005002636.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YV28"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01488">
        <property type="entry name" value="Uba3_RUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000106">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.20:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014929">
        <property type="entry name" value="E2-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030468">
        <property type="entry name" value="Uba3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08825">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01181">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E2 binding" evidence="12">
        <location>
            <begin position="374"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="463" mass="52007" checksum="44DED19B3339453B" modified="2020-06-17" version="1">VLLISYREKKRRRIEDLTEKMAVDGGHCDISCDWDGRWNHVRKFLERPGPFTHPDFEPSTECRLVKYLLTCKILVIGAGGLGCELLKNLALSGFRLIHVVDMDTIDVSNLNRQFLFRPKDVGRPKAEVAADFINGRIPGCKVVPHFKKIQDFDESFYRQFHIIVCGLDSVIARRWMNGMLISLLSYEDGILDPSSIIPLIDGGTEGFKGNARVILPGMTACIDCTLELYPPQINFPMCTIASMPRLPEHCIEYARILQWPKEKPFGEISLDGDNPEHIQWVFDKAQERAAEFNITGVTYRLTQGVVKRIIPAVASTNAVIAAACATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCSACSQVPQDLQFPPSAKLQEVLEYLTENASLQMKSPAITTTLEGKNKTLYLQSVKSIEERTRPNLCKTLKELGLSDGQELAVADVTTPQTVLFKLNFTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YVW9</accession>
    <name>A0A672YVW9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YVW9"/>
    <dbReference type="Ensembl" id="ENSSORT00005009039.1">
        <property type="protein sequence ID" value="ENSSORP00005008738.1"/>
        <property type="gene ID" value="ENSSORG00005004821.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YVW9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="108" mass="11778" checksum="2ABC77027EFB9E6C" modified="2020-06-17" version="1">ASQVTVTQPGAVSSDLGASATITCTTSQNVYAAGRYHYLHWYQQRDGETPKLLIYRATTRESGISSRFSGSGSNSHFTLTISDVQAEDAAVYYCQSYHVINSQAVFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWI8</accession>
    <name>A0A672YWI8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heat shock 70 kDa protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hspa14</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YWI8"/>
    <dbReference type="FunCoup" id="A0A672YWI8">
        <property type="interactions" value="768"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009216.1">
        <property type="protein sequence ID" value="ENSSORP00005008914.1"/>
        <property type="gene ID" value="ENSSORG00005004869.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YWI8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10238">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000013">
        <property type="entry name" value="Heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000433">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin, Chain A, domain 4"/>
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    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
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        <property type="entry name" value="HSP70_peptide-bd_sf"/>
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        <property type="entry name" value="Hsp_70_fam"/>
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        <property type="entry name" value="HSPA14_NBD"/>
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        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
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        <property type="entry name" value="Actin-like ATPase domain"/>
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        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023186"/>
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        <source>
            <dbReference type="RuleBase" id="RU003322"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="54426" checksum="BBEA19361276C5FB" modified="2020-06-17" version="1">MAAIGVHFGYTCACVAIFKDGRADVVANDAGDRVTPAVVGYRDTEQIVGIAAKQGRVRNATNTVVKVKQVLGRSFDDPETQAHKVETKCQVNKPYYEIKVGDHTENIAPEDVAKLIFHKMKETAQSALDSDVTDAVITVPFEFAHAQKNALREAAEAAGFNVLRLIHEPAAALLAYNIGQDCPSGKSHVLVYKLGGTSLSVTVLQVNGGMFRVLNTHTDHSIGGESFTQALAQHLATEFKRSFRHDVSTNARAMLKLMNGADMAKHSLSSLGSANCFVDSLHDGIDFDCNVSRARFELLCSALFNKSIQPIRSLLQEAGLSTNDINKVVLCGGSARIPRLQQMIQEMFPEVELLSSAPPDEVIAVGAALEAGLLVGKDGLNGEESETVDVSAADILLKEVDECGATVFNVLLPSGTPLPARRHHVLSGGGDLSSLCLEIYQKCGTEEPEKLAKVRNTPSIILRDLQPKEENHDIDTTVTMKRDGSVHVSCVEQSSGRSEAISIAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YY16</accession>
    <name>A0A672YY16_9TELE</name>
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        <recommendedName>
            <fullName evidence="14">Netrin receptor UNC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">unc5d</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the unc-5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YY16"/>
    <dbReference type="FunCoup" id="A0A672YY16">
        <property type="interactions" value="434"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009817.1">
        <property type="protein sequence ID" value="ENSSORP00005009498.1"/>
        <property type="gene ID" value="ENSSORG00005004602.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YY16"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000001">
        <property type="entry name" value="Unc-5 netrin receptor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000002">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000008">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Unc-5 netrin receptor C"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000039">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
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    </dbReference>
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        <property type="entry name" value="Unc-5 netrin receptor D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Death_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
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        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057755">
        <property type="entry name" value="UNC5A-D-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033772">
        <property type="entry name" value="UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF5">
        <property type="entry name" value="NETRIN RECEPTOR UNC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25609">
        <property type="entry name" value="Unc5_NetrinR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17217">
        <property type="entry name" value="UPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 14" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="162"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="18">
        <location>
            <begin position="533"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="16">
        <location>
            <begin position="864"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="918"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="964" mass="106632" checksum="75E496A3C18F4FF1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1F9</accession>
    <name>A0A672Z1F9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoinositide interacting regulator of transient receptor potential channels</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z1F9"/>
    <dbReference type="Ensembl" id="ENSSORT00005011375.1">
        <property type="protein sequence ID" value="ENSSORP00005011001.1"/>
        <property type="gene ID" value="ENSSORG00005005947.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1F9"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028068">
        <property type="entry name" value="PIRT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100:SF3">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15099">
        <property type="entry name" value="PIRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="12895" checksum="53039F21F561FC3B" modified="2020-06-17" version="1">MLSSVVTTSTMEPSSQDADTGASTTLSPLQDVDPSPAPPCGLLYWRPLLAMAVGALMFGSGTVVSLLYFTRVGHVPYLLGPLFLSVGLMLLVTGLVWIPMLKQSLEHKKYRKISMKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1R2</accession>
    <name>A0A672Z1R2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z1R2"/>
    <dbReference type="Ensembl" id="ENSSORT00005011118.1">
        <property type="protein sequence ID" value="ENSSORP00005010755.1"/>
        <property type="gene ID" value="ENSSORG00005005826.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1R2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="29763" checksum="2BE3F943C10AE4F8" modified="2020-06-17" version="1">DLELDLDSTPFIVQSSRLSCSLGPGSGSRLTCSLGSGSGSRLTCSLGPGSGSRLTCSGSGSRLTCSLGSGSGSRLTCSLGPGSGSRLSCSLGPGSGSRLTCSGSGSRLTCSLGSGSGSRLTCSLGPGSGSRLSCSLGPGSGSRLTCSLGSGSGSRLTCSLGPGSGSRLTCSLGPGFGSRLTCSLGSGSGSRLSCSLGPGSGSRLTCSLGPGSGSRLTCSLGPGSGSRLTCSLGSGSGSRLTCSLGPGSGSRLSCSLGPGSGSRLSCSLGSGSGSRLTCSLGPGSGSRLTCSLGSGSGSRLTCSLGPGSGSRLTCSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z295</accession>
    <name>A0A672Z295_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z295"/>
    <dbReference type="Ensembl" id="ENSSORT00005011257.1">
        <property type="protein sequence ID" value="ENSSORP00005010883.1"/>
        <property type="gene ID" value="ENSSORG00005005898.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z295"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF8">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="129"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="453" mass="50543" checksum="D6ADAB927CE15788" modified="2020-06-17" version="1">MRKLLYSLTSTRRVPYQKKTATCGNLSFLSRNMCTMRHAEEEDPAGFLPDEDRLQNVDLAPEASSSNRSDLHARFTADLNKVLMSPQEYRAMMRSLNSKQQEVVMFHRKWCKDAIFAMKHDRPMPQFKVYLSGPGGVGKSHIIKLVHYLTVLLTAFTGTAAFGIEGMTLHSSLGFTSGPKNKKEYQPAGSEKLNTLRSQLSKLKLLIIDEVSMVGADLLYHVHRRLQDICETSDPDSRFGGVSVLAVGDLYQLQPVGQNHVFGLPSDSYARLHGSLWEENFRLMELTESMRQRDDQHFAQLLMRVRTATCTQGDITLLKTRVVKKSDNSYPANALHVFKTNKEVDQHNAEHLKKLPNPVFDIKAVDQKKDVQTGLIDVVISTKPSDTGGLREVISVAVGARVMVTVNIDVSDGLENGVCGTVVGIENTGSTVHIIFAVPIKRQDVQFFAGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3D3</accession>
    <name>A0A672Z3D3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber evidence="6">2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">GlcNAc-1-P transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">dpagt1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:13290"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.15" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z3D3"/>
    <dbReference type="FunCoup" id="A0A672Z3D3">
        <property type="interactions" value="1030"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011690.1">
        <property type="protein sequence ID" value="ENSSORP00005011310.1"/>
        <property type="gene ID" value="ENSSORG00005006060.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3D3"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06855">
        <property type="entry name" value="GT_GPT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048439">
        <property type="entry name" value="DPAGT1_ins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033895">
        <property type="entry name" value="GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571:SF0">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21383">
        <property type="entry name" value="DPAGT1_ins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="DPAGT1 insertion" evidence="22">
        <location>
            <begin position="320"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF21383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="412" mass="46560" checksum="AE45F99D5B7EB4ED" modified="2020-06-17" version="1">MSGKMSPVPVLPLVINCFMSVLGCLATIKLIPAFKEHFISAKLYGMDLNKTSKKEVPESQGVISGTVFLIILFCFIPVPFLSCFVGDQCMGFPHDEFVQLIGALLAICCMIFLGFADDVLNLRWRHKLLLPTMASLPLLMVYFTNFGNTVIVVPKPFRALLGLHLDMGILYYVYMGMLAVFCTNAINILAGINGIESGQALFISGSIIIFNLLELSGDYRDDHVFSLYFMIPFFFTTLGLFYHNWYPSSVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFIPQVVNFIYSLPQLFHIIPCPRHRLPRLNPDTGKLGMSYSKFKRKDLSKLGHLILKVNVAELLKLLEVRRDPKGEDEFIECNNMTLINLVLKLFGPIHERNLTVIMLIIQVMGSTMAFGIRYHLVRLFYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3Q1</accession>
    <name>A0A672Z3Q1_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z3Q1"/>
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        <property type="protein sequence ID" value="ENSSORP00005011379.1"/>
        <property type="gene ID" value="ENSSORG00005006098.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
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        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
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    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="107" mass="11764" checksum="627163F720E735A5" modified="2020-06-17" version="1">MFCWDGQTLETSTFFHLSSSVVPGNTVSIRCKSSTGIGTYLHWYLQKEAESPKLLIYYSTSLQSGISSRFSGSGSGTDFTLTITDVQAEDAGVYYCHQGGYSFPFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3Q9</accession>
    <name>A0A672Z3Q9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Replication factor C subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Activator 1 subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rfc4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA. The RFC4 subunit probably functions as a scaffold on which the other complex components can assemble.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Subunit of the RFC complex, an heteropentameric complex consisting of a large subunit RFC1 and four small subunits RFC2, RFC3, RFC4 and RFC5; the RFC complex interacts with PCNA. Forms an heterotetrameric complex with RFC2, RFC3 and RFC5; this complex has ATPase activity but is not stimulated by PCNA. The heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 interacts with RAD17. Interacts with ATAD5. Interacts with CTF18. Interacts with CNTD1; this interaction facilitates crossover formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z3Q9"/>
    <dbReference type="Ensembl" id="ENSSORT00005011770.1">
        <property type="protein sequence ID" value="ENSSORP00005011389.1"/>
        <property type="gene ID" value="ENSSORG00005006049.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3Q9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000032">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000010">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="65"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="362" mass="40346" checksum="24A5266A34C66DEC" modified="2020-06-17" version="1">MQAFLKGATAQSTKPQKDKATAGPSTEKKAKAVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELYRQRVLELNASDERGIQVVREKVKNFAQLTVAGTRPDGKPCPPFKIIILDEADSMTAPAQAALRRTMEKESRTTRFCLICNYISRIIEPLTSRCSKFRFKPLANQIQEERLLDICEKENLKYTKEGITALVRVSEGDLRKAITFLQSAARLNVDKEITEHAIIEIAGVRLGRIMMDMIDNLLNICFRGTFEKLEVAVKNVVDEGYAATQILSQLHEAIIEQDLSDKQKSAITEKMAVVDKCLTDGADEYLQMLSLCSVIMQQASQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5S8</accession>
    <name>A0A672Z5S8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NudC domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nudcd1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z5S8"/>
    <dbReference type="Ensembl" id="ENSSORT00005012553.1">
        <property type="protein sequence ID" value="ENSSORP00005012158.1"/>
        <property type="gene ID" value="ENSSORG00005006438.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012554.1">
        <property type="protein sequence ID" value="ENSSORP00005012159.1"/>
        <property type="gene ID" value="ENSSORG00005006438.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037895">
        <property type="entry name" value="NUDCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21664">
        <property type="entry name" value="CHRONIC MYELOGENOUS LEUKEMIA TUMOR ANTIGEN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21664:SF1">
        <property type="entry name" value="NUDC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="7">
        <location>
            <begin position="275"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="389"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="585" mass="65879" checksum="5B9A8C65E47485C4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6E5</accession>
    <name>A0A672Z6E5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Acyl-coenzyme A thioesterase THEM4</fullName>
            <ecNumber evidence="19">3.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Thioesterase superfamily member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">them4</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40151"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40152"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:40140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>decanoyl-CoA + H2O = decanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40059"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27689"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:40060"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>dodecanoyl-CoA + H2O = dodecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:30136"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>hexadecanoyl-CoA + H2O = hexadecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16645"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="3.1.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:16646"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>octanoyl-CoA + H2O = octanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:30144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>tetradecanoyl-CoA + H2O = tetradecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:40120"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the THEM4/THEM5 thioesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.2" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z6E5"/>
    <dbReference type="Ensembl" id="ENSSORT00005012807.1">
        <property type="protein sequence ID" value="ENSSORP00005012410.1"/>
        <property type="gene ID" value="ENSSORG00005006532.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z6E5"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052365">
        <property type="entry name" value="THEM4/THEM5_acyl-CoA_thioest"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12418">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE THEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12418:SF19">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE THEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thioesterase" evidence="27">
        <location>
            <begin position="159"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="251" mass="27459" checksum="9FFFF099C2D3C0E7" modified="2020-06-17" version="1">MARSLGQMFKGFLRLGSGLTTRTPCPPGPGSTHRASLLCTTAALQSFLFPKPRDLSLPNSSWGPDMMRLYEHYNSLCEVETEGEKQPGPWRRLPSYNRILKYATGGVYLSKIIQSKARLFTRNIKQQGAAFEYVVFVNKEEQKCVCVFQAGHLLEGPPGHVHGGAIATMIDSVAGTHATYLSGLVLTANLNINYRSPISLGSTVVLESFLDKKEDRKTFISCKVTSADGSKLHTEATALFLSITVSNIIGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7F2</accession>
    <name>A0A672Z7F2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115431175</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030007269.1">
        <property type="nucleotide sequence ID" value="XM_030151409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z7F2"/>
    <dbReference type="FunCoup" id="A0A672Z7F2">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013716.1">
        <property type="protein sequence ID" value="ENSSORP00005013306.1"/>
        <property type="gene ID" value="ENSSORG00005006897.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431175"/>
    <dbReference type="InParanoid" id="A0A672Z7F2"/>
    <dbReference type="OrthoDB" id="6147321at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="339" mass="38386" checksum="00004996BDB9A905" modified="2020-06-17" version="1">MTNSTPFTQFILDAYLDVGLLKYLVFITVMCLYIVIIVSNVLLIVVICVNRSLHEPMYFFLCSLFVNELLGSTAVFPLLLTEILRDIHTIPVSFCFLQIFSIYSYANIELCILAAMSYDRYLSICHPLQYHTNMTSTKVAVLIALTWGFPVVEVGVMVSLSAPLHLCGNVIPKVYCDNYYIVKLACSDITVNNFYGIVYTVIIICSVLSVIIYSYSKILKVCFSGSKQTRHKAVSTCTPHIVSLLNFCFGCCFEIFQSRFNMTYLPTVLRVFLSLYFLTCQPLFSPLIYGLNLTKIRNICKSIFCVNRMFYNLSVSAELGSNRHRWLISASVLCCSVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z867</accession>
    <name>A0A672Z867_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial assembly of ribosomal large subunit protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">malsu1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Required for normal mitochondrial ribosome function and mitochondrial translation. May play a role in ribosome biogenesis by preventing premature association of the 28S and 39S ribosomal subunits. Interacts with mitochondrial ribosomal protein uL14m (MRPL14), probably blocking formation of intersubunit bridge B8, preventing association of the 28S and 39S ribosomal subunits. Addition to isolated mitochondrial ribosomal subunits partially inhibits translation, probably by interfering with the association of the 28S and 39S ribosomal subunits and the formation of functional ribosomes. May also participate in the assembly and/or regulation of the stability of the large subunit of the mitochondrial ribosome. May function as a ribosomal silencing factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030012899.1">
        <property type="nucleotide sequence ID" value="XM_030157039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z867"/>
    <dbReference type="FunCoup" id="A0A672Z867">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013462.1">
        <property type="protein sequence ID" value="ENSSORP00005013054.1"/>
        <property type="gene ID" value="ENSSORG00005006803.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434904"/>
    <dbReference type="CTD" id="115416"/>
    <dbReference type="InParanoid" id="A0A672Z867"/>
    <dbReference type="OrthoDB" id="21330at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000018">
        <property type="entry name" value="Mitochondrial assembly of ribosomal large subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="IOJAP SUPERFAMILY ORTHOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043:SF0">
        <property type="entry name" value="MITOCHONDRIAL ASSEMBLY OF RIBOSOMAL LARGE SUBUNIT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="242" mass="28024" checksum="9B9A09FB2E46891D" modified="2020-06-17" version="1">MSILNRLKPLLSSVVKQCILPEQTVFLRSVCTSPVSRSHRFTTHSSTCQSRYNVFTCTFSPAITKRCYSELYNENDKYVKSTSGAIQEELQEVEVEFSDDDPRSQRSSKAFTLDVLVSLLQQENAVDICVIKVPEQIKYAEYFVVVSGLSTRHLRAMALYAIKVYKFLKKDQHSHVKIEGKDTDDWLCIDFGSMVVHFMLPETREVYELEKLWTLRSYDEQLKNMPAETLPEDFIYDTEVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9Q3</accession>
    <name>A0A672Z9Q3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z9Q3"/>
    <dbReference type="Ensembl" id="ENSSORT00005013661.1">
        <property type="protein sequence ID" value="ENSSORP00005013253.1"/>
        <property type="gene ID" value="ENSSORG00005006865.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9Q3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025383075" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="16"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="193"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="334" mass="38392" checksum="B430B73DE1DC460E" modified="2020-06-17" version="1">MKLLLLLFFCHFSFSEKHTLSVLFTVSSEVQAFPEVIAVVQVDGVQVLYWDSNIKKVEPRQDWMKKLIEDDPQHSESLTQDEQIIRDQLTEVMQHLRQTEGVHVLQIRSGCEWDDETAAVNSVRQYGYDGVDFIKLDMKTQKWVALKPEADIIKQDWDKGTENNMRWTNHDTNICPQWLKKYLDLGNSSLHTPDFCSVFLLQKSPLAPVTCHVTGFHSGRAVMFWREDGGDVHEEVEPGEILPNGDGTYQMSVDLHVSSIAPENWKRYECVCQLRGVYETAVKLDKTLIKTNWNVLSEFPIGPVIGCVVGFLLLAVIVAGLIRWRMNRKTVYFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAU8</accession>
    <name>A0A672ZAU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="6 11">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ahcy</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine. Binds copper ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:21709"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="10 11">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="6 11"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZAU8"/>
    <dbReference type="FunCoup" id="A0A672ZAU8">
        <property type="interactions" value="1621"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014869.1">
        <property type="protein sequence ID" value="ENSSORP00005014446.1"/>
        <property type="gene ID" value="ENSSORG00005006775.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZAU8"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="3 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="13">
        <location>
            <begin position="201"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="167"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="232"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="309"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="441" mass="48649" checksum="11BD07D970E29707" modified="2020-06-17" version="1">SALVLSVDHQIQHNLLAASDISLADWGRKSIEIAENEMPGLMKMREMYGGSKPLKGARIAGCLHMTLQTAVLIETLTALGAEVQWSSCNIFSTHDHAASAIAKAGIPVYAWKGETDEEYVWCIEQTLYFKDGQPLNMILDDGGDLTNLIHTKYPKLLAGIRGVSEETTTGVHNLYKMFKKGELKIPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCVQALRAFGARVIVTEVDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCENIIMGHHFEKMKDDSIVCNIGHFDCEIDVNWLNKNAEKVEVKPQVHRYRLKSGNHIIILAEGRLVNLGCAMGHPSFVMSNSFTNQVLAQIELWTNTDKYPVGVYFLPKRLDEQVAAAHLDKLGVKLTKLTEQQAKYLGVPVDGPFKPDHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFG5</accession>
    <name>A0A672ZFG5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Immunoglobulin-like domain-containing receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115412395</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. LISCH7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029980773.1">
        <property type="nucleotide sequence ID" value="XM_030124913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZFG5"/>
    <dbReference type="FunCoup" id="A0A672ZFG5">
        <property type="interactions" value="960"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016536.1">
        <property type="protein sequence ID" value="ENSSORP00005016036.1"/>
        <property type="gene ID" value="ENSSORG00005008098.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412395"/>
    <dbReference type="InParanoid" id="A0A672ZFG5"/>
    <dbReference type="OrthoDB" id="9944507at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070506">
        <property type="term" value="F:high-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051874">
        <property type="entry name" value="Ig-like_domain-LISCH7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008664">
        <property type="entry name" value="LISCH7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15923:SF6">
        <property type="entry name" value="IMMUNOGLOBULIN-LIKE DOMAIN CONTAINING RECEPTOR 1B PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15923">
        <property type="entry name" value="TRANSMEMBRANE AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05624">
        <property type="entry name" value="LSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025549193" evidence="13">
        <location>
            <begin position="20"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="25"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="374"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="392"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="412"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="553" mass="61816" checksum="10253BC38E7AD528" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGJ1</accession>
    <name>A0A672ZGJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Histone H4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 14">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZGJ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005016904.1">
        <property type="protein sequence ID" value="ENSSORP00005016395.1"/>
        <property type="gene ID" value="ENSSORG00005008324.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016905.1">
        <property type="protein sequence ID" value="ENSSORP00005016396.1"/>
        <property type="gene ID" value="ENSSORG00005008325.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000012">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0164">Citrullination</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 14" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="15">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="92" mass="10311" checksum="B431422CD5D64F4E" modified="2020-06-17" version="1">ASGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHY3</accession>
    <name>A0A672ZHY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZHY3"/>
    <dbReference type="FunCoup" id="A0A672ZHY3">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016877.1">
        <property type="protein sequence ID" value="ENSSORP00005016369.1"/>
        <property type="gene ID" value="ENSSORG00005008305.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZHY3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="136"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="223"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="353" mass="38906" checksum="0708D317393BDF9A" modified="2020-06-17" version="1">MLMFSSPVAPTVPSVSFSPSGEIVEGSSVTLTCTSDANPPVTKYTWYKRGNNRLQSRGEGQQFVLSFIKSSDSGSYSCVAENSLGRKMSEYIYIDVKCEILCSLEILMQQRINKGTIFIHSFIHSVKSPPDPPRLPSVSVSPSGQIVEGSSVTLTCTSDANPPVTKYTWYKRGKNQPQSGGEGQQFVLSFIKSSDSGLYSCVAENSLEQKMSKFVHIDVKLSPPLLPSGQIVQGSSVTLTCTSDANPPVTKYTWYKEDEDSPKASGQNFTITDFRSEHSGNYYCEAQNSRGRQNSASHLIVVSCDYSTPWMFSSFIAILTIMIVRGIFSVYVHQQMSIPMYLVYVCSSVLCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZK09</accession>
    <name>A0A672ZK09_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gmcl1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZK09"/>
    <dbReference type="FunCoup" id="A0A672ZK09">
        <property type="interactions" value="1486"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018004.1">
        <property type="protein sequence ID" value="ENSSORP00005017485.1"/>
        <property type="gene ID" value="ENSSORG00005008544.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZK09"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007281">
        <property type="term" value="P:germ cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18495">
        <property type="entry name" value="BACK_GCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18305">
        <property type="entry name" value="BTB_POZ_GCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000092">
        <property type="entry name" value="Germ cell-less, spermatogenesis associated 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043380">
        <property type="entry name" value="Gcl-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23231:SF17">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23231">
        <property type="entry name" value="GERM CELL-LESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="96"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="BACK" evidence="7">
        <location>
            <begin position="201"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="519" mass="59734" checksum="1876B918AE71F4D5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLP1</accession>
    <name>A0A672ZLP1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">emc2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Peripheral membrane protein</topology>
            <orientation evidence="1 8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMC2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZLP1"/>
    <dbReference type="FunCoup" id="A0A672ZLP1">
        <property type="interactions" value="1363"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018641.1">
        <property type="protein sequence ID" value="ENSSORP00005018100.1"/>
        <property type="gene ID" value="ENSSORG00005008901.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLP1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000074">
        <property type="entry name" value="ER membrane protein complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="EMC2 TPR-like" evidence="9">
        <location>
            <begin position="98"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="163"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="305" mass="35314" checksum="92274C0EC88949F0" modified="2020-06-17" version="1">MPLIPWLGLDHICFKVTLFSTEMRDKLRKWREDNFRNSEQIVDVGEELISEHGSKLGDDIWIIYEQVMIAALDCSRDDLALTCLQELRKQFPDSHRVKRLSGMRLEALERYDEANKHYDAILQEDPTNTAARKRKISILKAQGKSTEAIRELNEYLEQFVGDQEAWHELSELYINEHDYGKAAFCLEELMMTNPHNHLYCEQYAEVKYTQGGLENLELSRKYFAQALRLNNRNMRALFGLYMSASHIAASPKVSAKVKKDNMKYASWAASQINRAYKLAGRGTKENKCSVKAVEEMLESMQITMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZMN7</accession>
    <name>A0A672ZMN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZMN7"/>
    <dbReference type="Ensembl" id="ENSSORT00005018975.1">
        <property type="protein sequence ID" value="ENSSORP00005018431.1"/>
        <property type="gene ID" value="ENSSORG00005009116.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZMN7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000151">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000185">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="70" mass="8264" checksum="05F0E155343FEFDF" modified="2020-06-17" version="1">IHLSIHTGERPYQCNQCNKRFSLRFMLMKHLRVHTGERPFQCSHCPKRFTLVSVLARHERINNNATSVNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTR7</accession>
    <name>A0A672ZTR7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zgc:175088</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fbxo2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZTR7"/>
    <dbReference type="FunCoup" id="A0A672ZTR7">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021130.1">
        <property type="protein sequence ID" value="ENSSORP00005020556.1"/>
        <property type="gene ID" value="ENSSORG00005010000.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZTR7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000012">
        <property type="entry name" value="F-box only protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007397">
        <property type="entry name" value="F-box-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039752">
        <property type="entry name" value="F-box_only"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125:SF11">
        <property type="entry name" value="F-BOX ONLY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125">
        <property type="entry name" value="F-BOX ONLY PROTEIN 6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04300">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01198">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51114">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="167" mass="18961" checksum="EA06749824791896" modified="2020-06-17" version="1">KKLNELEFWELTENGGSQWKVEDMPGDCGHDFSSDGVSKYFATSFELCLKRQVIDLLAEGYSADQLDAQPAVTVEDWYCGRTDCGCTYQLSVDLLDENQEVIQEFRSEPVTLDPDCDDCSWKQVSHTFTDYGPGLRFISWEHGGQDTKFWEGWFGVRITGSSLTIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A007</accession>
    <name>A0A673A007_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SH2 domain-containing adapter protein D-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">shdb</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A007"/>
    <dbReference type="Ensembl" id="ENSSORT00005023466.1">
        <property type="protein sequence ID" value="ENSSORP00005022800.1"/>
        <property type="gene ID" value="ENSSORG00005011058.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A007"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09945">
        <property type="entry name" value="SH2_SHB_SHD_SHE_SHF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000021">
        <property type="entry name" value="Putative SH2 domain-containing adapter protein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051846">
        <property type="entry name" value="SH2_domain_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127">
        <property type="entry name" value="HEAVYWEIGHT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127:SF33">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING ADAPTER PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="296"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="41"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="165"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="396" mass="44588" checksum="AA63A8F048020DCA" modified="2020-06-17" version="1">MAKWLKDYLSFGSRKVPPQPPTPDYTESEILKAYRLQRDLDFEDPYEDSETRSSRGESGASDPATPVYGSPMKTSSTDVKSPKHRLIKVDSQELGRTKILLSAISLEDQQEPVVPSAPLAGDTDYSDPFDARLDHRPEPDLGPIPCENNGYMEPYEAQRVITELQRGPGGGRARGGVQLYDTPYDDERGQRPGLMYGEPQEEGKESSRLPQDDERPADEYDQPWEWKKDHISKAFAVQFDGADWERSSSPTERLRPPGPRSSPLAGGGMKFRMPQEGLAMVGERVDPTLPLEKQVWYHGSLSRSDAESLLTLCKECSYLVRNSQTNRSDYSLSLRSCQGFMHMKFTQCKDGKYVLGQNSPPFDTIPEVIHFYTTHKLPIRGAEHLSLLFPVLVQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3M4</accession>
    <name>A0A673A3M4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tcf7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A3M4"/>
    <dbReference type="Ensembl" id="ENSSORT00005023926.1">
        <property type="protein sequence ID" value="ENSSORP00005023252.1"/>
        <property type="gene ID" value="ENSSORG00005011114.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A3M4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010456">
        <property type="term" value="P:cell proliferation in dorsal spinal cord"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060847">
        <property type="term" value="P:endothelial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021903">
        <property type="term" value="P:rostrocaudal neural tube patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21996">
        <property type="entry name" value="HMG-box_TCF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000001">
        <property type="entry name" value="transcription factor 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000002">
        <property type="entry name" value="transcription factor 7-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013558">
        <property type="entry name" value="CTNNB1-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF33">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01366">
        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="HMG box" evidence="12">
        <location>
            <begin position="279"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="10">
        <location>
            <begin position="279"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="349"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="411" mass="46196" checksum="FEAB5AB81DE177D0" modified="2020-06-17" version="1">MPQLSSGGGDDLGANDEMIAFKDEGEQEEKIQENAFTERDLADLKSSLVNESEINQSPNAAAVRRGQQGEQRGFPDKHREHLDGVSKQQDGGMYKSPAYPGYPFLMLPDPYLPNSSVSPSSNKVSVVQQSHGMHPLTSLLPYSNDHFSPSPPHLPTDMTQKTGVHRHQSQDLSGYYSLPPGGVGQITPSMGWQSQPVYPGLSSCGFRQSYSSNLSSASSYSRFPHSLMLGPSGMHPTGIPHPAIVPPTGKQEHDQYDRSMYVKPQMEAKREKEPKKPVIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALTREEQAKYYELARKERQLHMQLYPTWSARDNYGKKKRRKREKQQDSNTDPGSPKKCRARFGLNQQTDWCGPCRCVPNFHHHPTILSSLILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4G2</accession>
    <name>A0A673A4G2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sal-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Sal-like protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">sall3a</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable transcription factor.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Transcriptional repressor involved in organogenesis. Plays an essential role in ureteric bud invasion during kidney development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">May associate with NuRD histone deacetylase complex (HDAC). Interacts with components of HDAC complex including HDAC1, HDAC2, RBBP4, RBPP7, MTA1 and MTA2. Interacts with CCNQ. Interacts with NSD2 (via PHD-type zinc fingers 1, 2 and 3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005024203.1">
        <property type="protein sequence ID" value="ENSSORP00005023522.1"/>
        <property type="gene ID" value="ENSSORG00005011393.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4G2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003337">
        <property type="term" value="P:mesenchymal to epithelial transition involved in metanephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021772">
        <property type="term" value="P:olfactory bulb development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd12906">
        <property type="entry name" value="SPRY_SOCS1-2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000007">
        <property type="entry name" value="SPRY domain-containing SOCS box protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF3">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="6">
        <location>
            <begin position="35"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="7">
        <location>
            <begin position="238"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="31453" checksum="C4B7EA826102F715" modified="2020-06-17" version="1">MGQKISGSIKSVDVRGEPSYRPVRRELRGPDFCRPPRLDLLLDMPPASPETQLRHAWNPDDRSLNVFVKEDDKLTFHRHPVAQSTDCIRGKVGYTRGLHVWRIHWPARQRGTHAVVGVATAEAPLHSVGYTALVGSDSESWGWDLGRNRLYHDGKNRPVSTSAPTYPCFLEPDESFVLPDSLTVILDMDEGTLSFMVDGQYLGVAFRGLKGRRLYPIVSAVWGHCEVSIRYVNGLDPEPLPLMDLCRRVARLALGRERIHHIDTLPLPQTLKNYLQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAM7</accession>
    <name>A0A673AAM7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">2-amino-3-carboxymuconate-6-semialdehyde decarboxylase</fullName>
            <ecNumber evidence="4 12">4.1.1.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Picolinate carboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">acmsd</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Converts alpha-amino-beta-carboxymuconate-epsilon-semialdehyde (ACMS) to alpha-aminomuconate semialdehyde (AMS). ACMS can be converted non-enzymatically to quinolate (QA), a key precursor of NAD, and a potent endogenous excitotoxin of neuronal cells which is implicated in the pathogenesis of various neurodegenerative disorders. In the presence of ACMSD, ACMS is converted to AMS, a benign catabolite. ACMSD ultimately controls the metabolic fate of tryptophan catabolism along the kynurenine pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-amino-3-carboxymuconate 6-semialdehyde + H(+) = 2-aminomuconate 6-semialdehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:16557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77634"/>
            <dbReference type="ChEBI" id="CHEBI:77803"/>
            <dbReference type="EC" id="4.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Secondary metabolite metabolism; quinolate metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. ACMSD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.45" evidence="4 12"/>
    <dbReference type="AlphaFoldDB" id="A0A673AAM7"/>
    <dbReference type="Ensembl" id="ENSSORT00005027410.1">
        <property type="protein sequence ID" value="ENSSORP00005026630.1"/>
        <property type="gene ID" value="ENSSORG00005012752.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AAM7"/>
    <dbReference type="UniPathway" id="UPA00270"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001760">
        <property type="term" value="F:aminocarboxymuconate-semialdehyde decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904985">
        <property type="term" value="P:negative regulation of quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF27">
        <property type="entry name" value="2-AMINO-3-CARBOXYMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="13">
        <location>
            <begin position="60"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="346" mass="38994" checksum="BA27C2727ACE0155" modified="2025-10-08" version="2">MNINVTNHTLKSTAWCFCVVNHTHCYLLQRYGYGGFVQLHHHCKDEAKMMKDGKLFRVIQENCWDACARIRDMDQYGVSVQALSTVPVMFSYWAKPHDTLDLCGLLNDGLAQTVRAHPKRFVGLGTLPMQAPDLAVVEMTRCVNELGFPGVQIGSHINDLDLNAVELHPFYAAAEELGCSIFVHPWDMQTDGRMAKYWLPWLVGMPSETTMAICSMIFGGIFERFPKLKVCFAHGGGSFPFTVGRIEHGHKVRPDLCAVDNKFNPRKYLGSFYTDSLVHDPISLKLLIDIIGKDKVMLGTDYPFPLGELQPGSLIESMDEFDDTLKDKLLAVNALEFLGLKREQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABM9</accession>
    <name>A0A673ABM9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Exostosin-1c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ext1c</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Glycosyltransferase required for the biosynthesis of heparan-sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>3-O-{[(1-&gt;4)-beta-D-GlcA-(1-&gt;4)-alpha-D-GlcNAc](n)-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl}-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{alpha-D-GlcNAc-[(1-&gt;4)-beta-D-GlcA-(1-&gt;4)-alpha-D-GlcNAc](n)-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl}-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16213"/>
            <dbReference type="Rhea" id="RHEA-COMP:12621"/>
            <dbReference type="Rhea" id="RHEA-COMP:12623"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132415"/>
            <dbReference type="ChEBI" id="CHEBI:132416"/>
            <dbReference type="EC" id="2.4.1.224"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-O-{alpha-D-GlcNAc-[(1-&gt;4)-beta-D-GlcA-(1-&gt;4)-alpha-D-GlcNAc](n)-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl}-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-{[(1-&gt;4)-beta-D-GlcA-(1-&gt;4)-alpha-D-GlcNAc](n+1)-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl}-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12623"/>
            <dbReference type="Rhea" id="RHEA-COMP:14295"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132415"/>
            <dbReference type="ChEBI" id="CHEBI:132416"/>
            <dbReference type="EC" id="2.4.1.225"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ABM9"/>
    <dbReference type="FunCoup" id="A0A673ABM9">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027453.1">
        <property type="protein sequence ID" value="ENSSORP00005026671.1"/>
        <property type="gene ID" value="ENSSORG00005012736.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABM9"/>
    <dbReference type="OrthoDB" id="5954868at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050508">
        <property type="term" value="F:glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050509">
        <property type="term" value="F:N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000034">
        <property type="entry name" value="Exostosin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261">
        <property type="entry name" value="ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261:SF3">
        <property type="entry name" value="EXOSTOSIN GLYCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="20">
        <location>
            <begin position="107"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="21">
        <location>
            <begin position="475"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="740" mass="86194" checksum="4278454C9E954ECF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AF95</accession>
    <name>A0A673AF95_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TFIIS central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AF95"/>
    <dbReference type="Ensembl" id="ENSSORT00005028770.1">
        <property type="protein sequence ID" value="ENSSORP00005027969.1"/>
        <property type="gene ID" value="ENSSORG00005013395.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AF95"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF13">
        <property type="entry name" value="DEATH-INDUCER OBLITERATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS central" evidence="2">
        <location>
            <begin position="159"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="647" mass="70240" checksum="F213B3EC7793B31A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AG63</accession>
    <name>A0A673AG63_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Activator of 90 kDa heat shock protein ATPase homolog 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115415569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029985054.1">
        <property type="nucleotide sequence ID" value="XM_030129194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AG63"/>
    <dbReference type="Ensembl" id="ENSSORT00005028163.1">
        <property type="protein sequence ID" value="ENSSORP00005027377.1"/>
        <property type="gene ID" value="ENSSORG00005013059.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415569"/>
    <dbReference type="InParanoid" id="A0A673AG63"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08892">
        <property type="entry name" value="SRPBCC_Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.20:FF:000001">
        <property type="entry name" value="Activator of 90 kDa heat shock protein ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="ASHA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF33">
        <property type="entry name" value="AHA1, ACTIVATOR OF HEAT SHOCK PROTEIN ATPASE HOMOLOG 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="29"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="336" mass="38174" checksum="689101A8ACD29A85" modified="2020-06-17" version="1">MAKWGEGDPRWIVEERADATNVNNWHWTERDVTSWSSERLRKLLLGVQVQGSEGVCQVTDVSKLDGEASINNRKGKLIFFYEWQLRANWLGTTSSGVKYRGTVEVSNLSDENDEDDLDITVSLCKDQPDTPLLQLMKKNGTKEVRKVLGEYVKQLKSEFSQGMILPTANGPQAPPPPKKTQIQTSKMQIQTTKTQTSSAHSQMGIRIPTCNFSLTETFLTSADELYRTFINQEFVQVFTRSAAVVDGRRGGRFQLLDGSVSGEFTNLVPDQKIEMRWRFRTWPSEHYASVSLQLEEQGGETELKMECVGVPSGEEDSTREGWKRFYFQAIKQTFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGC6</accession>
    <name>A0A673AGC6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AGC6"/>
    <dbReference type="Ensembl" id="ENSSORT00005029108.1">
        <property type="protein sequence ID" value="ENSSORP00005028299.1"/>
        <property type="gene ID" value="ENSSORG00005013545.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGC6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF4">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="82" mass="8618" checksum="9A69B0141F8DA734" modified="2020-06-17" version="1">MATLFPNGILFDGIVYRILPGGYAVIGAAAMTGAVTHTVSTAVICFELTGQISHILPMMVAVILANMVAQAITPEEDRLCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIC4</accession>
    <name>A0A673AIC4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Sodium/potassium/calcium exchanger 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">slc24a2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AIC4"/>
    <dbReference type="Ensembl" id="ENSSORT00005030293.1">
        <property type="protein sequence ID" value="ENSSORP00005029460.1"/>
        <property type="gene ID" value="ENSSORG00005014070.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AIC4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060292">
        <property type="term" value="P:long-term synaptic depression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060291">
        <property type="term" value="P:long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000002">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000004">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF41">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="464"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="566"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="596"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
        <location>
            <begin position="140"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
        <location>
            <begin position="465"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="626" mass="69127" checksum="5C8F585FDA8673CF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJC1</accession>
    <name>A0A673AJC1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isochorismatase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">isoc2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030013799.1">
        <property type="nucleotide sequence ID" value="XM_030157939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AJC1"/>
    <dbReference type="Ensembl" id="ENSSORT00005030277.1">
        <property type="protein sequence ID" value="ENSSORP00005029444.1"/>
        <property type="gene ID" value="ENSSORG00005014056.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030279.1">
        <property type="protein sequence ID" value="ENSSORP00005029446.1"/>
        <property type="gene ID" value="ENSSORG00005014056.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435490"/>
    <dbReference type="CTD" id="79763"/>
    <dbReference type="OrthoDB" id="269496at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="CDD" id="cd01012">
        <property type="entry name" value="YcaC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.850:FF:000001">
        <property type="entry name" value="Isochorismatase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050993">
        <property type="entry name" value="Isochorismatase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119:SF3">
        <property type="entry name" value="ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="12"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="197" mass="21549" checksum="22827A76A5D7050D" modified="2020-06-17" version="1">MAGIGRLATKDSVLFLCDMQEKFRPNIFQFTNIVSNAARLLQASRILAIPPILTEQYPKGLGPTVPELGAEDLKAHAKTTFTMMIEEVEKELQALGNPKQAILCGIEAHACIACTTFDLLEKGMEVHIVADAVSSRSQTDRLFALSRLKQSGAYLTTTEAVLLQLVQDAKHPNFKEIQKLLAHPSPDTGLLAFFSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALZ7</accession>
    <name>A0A673ALZ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Interleukin 11a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">il11a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ALZ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005030260.1">
        <property type="protein sequence ID" value="ENSSORP00005029427.1"/>
        <property type="gene ID" value="ENSSORG00005014045.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALZ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020438">
        <property type="entry name" value="IL-11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022356">
        <property type="entry name" value="IL-11_fish"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020427">
        <property type="entry name" value="IL-11A_fish"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16922">
        <property type="entry name" value="INTERLEUKIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16922:SF0">
        <property type="entry name" value="INTERLEUKIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07400">
        <property type="entry name" value="IL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01945">
        <property type="entry name" value="IL11AFISH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01944">
        <property type="entry name" value="INTLKN11FISH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01927">
        <property type="entry name" value="INTRLEUKIN11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025621979" evidence="1">
        <location>
            <begin position="27"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="201" mass="23260" checksum="12A4BA318E4FDF32" modified="2020-06-17" version="1">PTVLLDSSSSLLFSLLLAQLPVFTSASPVPHRRPSDMDRLSNQTKNLMKLTQELLREHAFDSDVEPHRFSSLPEMSNRSANDLNNLELKPTLSQLHADLKLYEHHFEWLNKVSKKHHHPALPKLVEMIREMKSLINLLHRQMLRVEAPRLTPATPSLPPHLPYQFDVLQSTHELLQHFKLFCDWAYRAFISLKPKVTVVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANB8</accession>
    <name>A0A673ANB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ubl3b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ANB8"/>
    <dbReference type="FunCoup" id="A0A673ANB8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030941.1">
        <property type="protein sequence ID" value="ENSSORP00005030098.1"/>
        <property type="gene ID" value="ENSSORG00005014373.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ANB8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17048">
        <property type="entry name" value="Ubl_UBL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000167">
        <property type="entry name" value="Ubiquitin-like 3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017000">
        <property type="entry name" value="MUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040015">
        <property type="entry name" value="UBL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047977">
        <property type="entry name" value="UBL3_Ubl_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032572">
        <property type="entry name" value="MUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR032572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="125" mass="13983" checksum="E271DF2746BBAAA6" modified="2020-06-17" version="1">MSEEVRGSRGIPPLINDVHLRLILVSGKTQDFTFSPNDSATDIAKHVFENWPAGWEEERVSSPSILRLIFQGRFLHGNVTLGALKLPPGRTTVMHLVARETLPEPNSHGQRNREKTTESNCCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANQ7</accession>
    <name>A0A673ANQ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cell adhesion molecule 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cadm2a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ANQ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005032190.1">
        <property type="protein sequence ID" value="ENSSORP00005031315.1"/>
        <property type="gene ID" value="ENSSORG00005014723.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ANQ7"/>
    <dbReference type="OrthoDB" id="5843397at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF1">
        <property type="entry name" value="CELL ADHESION MOLECULE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="36"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="136"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="233"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="438" mass="47685" checksum="900FAAC294195C69" modified="2020-06-17" version="1">MNIKSNLIFTLYTIYGILLKVMASKGKVRGLSGQYPLVQNMTVTEGGTVNMTCRVEYNDNTSLQWSNPAQQTLFFGDKKALRDNRIELVRASWTELTISISDITISDEGMYTCSLFTMPVRTAKAYLTVLGVPEKPEIDGLTKPALEGDQITLTCITQGSKPAADLRWFRNEKEVKGAKEVNPSGKTFTVRSSLQLHVDRNDDGVAYTCRVDHVALAQSPQLATEVLEVHYAPRVEIIHSLAVPQEGQNLKLECVSKGNPSPDPVMWTKDGGELPDLDRMIVDGRELTFTSLNKTDNGTYRCEASNHLGTSSAEYVLYVYDAPTTPPPSTTAPLHPIPPDSTALLGTHASDPTVTGNGDPNALGQHGTDHALIGGVVAVVVFVTLCLIIVLGRYLARHKGTYLTNEAKGADDAPDADTAIINAEGNHAHAEEKKEYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQD1</accession>
    <name>A0A673AQD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein phosphatase with EF-hands</fullName>
            <ecNumber evidence="10">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ppef1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A673AQD1"/>
    <dbReference type="Ensembl" id="ENSSORT00005032363.1">
        <property type="protein sequence ID" value="ENSSORP00005031484.1"/>
        <property type="gene ID" value="ENSSORG00005014986.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQD1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050906">
        <property type="term" value="P:detection of stimulus involved in sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012008">
        <property type="entry name" value="Ser/Thr-Pase_EF-hand_contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE WITH EF-HANDS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000912">
        <property type="entry name" value="PPEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025408395" description="Serine/threonine-protein phosphatase with EF-hands" evidence="13">
        <location>
            <begin position="16"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="619"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="659"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="709" mass="81030" checksum="CF6B77B7E6A556C5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQL2</accession>
    <name>A0A673AQL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Titin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AS67</accession>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </feature>
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        </location>
    </feature>
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            <end position="599"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="732"/>
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    </feature>
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    </feature>
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            <begin position="558"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
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            <begin position="568"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="649"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="669"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="967" mass="105698" checksum="CECF2A2859D6B6A6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATH4</accession>
    <name>A0A673ATH4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chromobox homolog 7b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbx7b</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ATH4"/>
    <dbReference type="Ensembl" id="ENSSORT00005032504.1">
        <property type="protein sequence ID" value="ENSSORP00005031622.1"/>
        <property type="gene ID" value="ENSSORG00005015049.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATH4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043000">
        <property type="entry name" value="CBX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033773">
        <property type="entry name" value="CBX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47277">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47277:SF1">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17218">
        <property type="entry name" value="CBX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="132" mass="15344" checksum="2657F2E7890FDAB7" modified="2020-06-17" version="1">MELSAIGEQVFAVESIVKKRVRKVMYSTWEPEEHILDQRLVQAYEEKEQRDRVLGHRRKGSKAKKLLLQVLDLKKDEVTAASEKPYDDDNVWRPVVRPGEVTVTDVTINSLTVTFRESRVAKGFFRDWGLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AU20</accession>
    <name>A0A673AU20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">atp5f1c</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Subunit gamma, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the central stalk subunit delta, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Interacts with FLVCR2; this interaction occurs in the absence of heme and is disrupted upon heme binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030006234.1">
        <property type="nucleotide sequence ID" value="XM_030150374.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030006235.1">
        <property type="nucleotide sequence ID" value="XM_030150375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AU20"/>
    <dbReference type="Ensembl" id="ENSSORT00005033995.1">
        <property type="protein sequence ID" value="ENSSORP00005033091.1"/>
        <property type="gene ID" value="ENSSORG00005015693.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430387"/>
    <dbReference type="CTD" id="509"/>
    <dbReference type="InParanoid" id="A0A673AU20"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000007">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000013">
        <property type="entry name" value="ATP synthase subunit gamma, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11 15" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="293" mass="32109" checksum="441949D26E73E345" modified="2020-06-17" version="1">MFARTSALVFSPQCGQVRNMATLKDITIRLKSIKNIQKITKSMKMVAAAKYARAERQLKPARIYGNGALALYEKADIKAPEEKATKHLIIGVTSDRGLCGAIHSGVAKTIKNEIATLTGAGKEVMVVNVGDKLRGLLHRTHGKHIMLNCKEVGRKPPSFGDASVIATELLNSGYEFDQGAVIFNRFRSVISYKTDQKPVFSTDTVATSENMGVYDDIDADVLRNYQEFALVNIIYLALRESSTSEQSARMTAMDSASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWX8</accession>
    <name>A0A673AWX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nop53</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NOP53 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030007537.1">
        <property type="nucleotide sequence ID" value="XM_030151677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AWX8"/>
    <dbReference type="Ensembl" id="ENSSORT00005034677.1">
        <property type="protein sequence ID" value="ENSSORP00005033764.1"/>
        <property type="gene ID" value="ENSSORG00005015989.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431365"/>
    <dbReference type="CTD" id="29997"/>
    <dbReference type="InParanoid" id="A0A673AWX8"/>
    <dbReference type="OrthoDB" id="5072at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017302">
        <property type="entry name" value="Gltscr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="483" mass="55983" checksum="0BCD654B2C40278E" modified="2020-06-17" version="1">MALPLYPERDSQKKKSEIPFCFPNMAAARRLKRVVASQPGFLPIKSPSEPVSTRRKRVNKNRKKNWNKYSDINDVEEFLEDVRLQERTTGGLLSEKTDDSLFFLDAGQPEKVEQEAPGPAEGKKRKGKASRPLRIDLILQHDSLVPPPKDVLAYQQPNAKKLRRIAQKAEQLAAKGVVPRRQKQLLNRRPVERTVKKAVTEANNNPDRDYYDIWGQESKDSGDPWYLQQTRKKLVKRPEKLNEKPSVLPAVEVIAPGGSYNPDFFSHQALLQEAHEVEVKKQKEEDKIVRQITVNKEDTATEETIFQEQVEGLVEEENEEEETAPDDEEESVVVGAIAVAEKKTERQRKRQRADKIKEQQRLADRRQVDQRQQLFQLRSIKASIKQQEKATAVRQIKRKVKQEAQKAQPRRLGRLKFQPQDLEVQLSDELAGSLRQLKPEGSVLKDRFKSLQKRNLIEPRERAKFKRRHKVKYVEKRAFRAIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZF8</accession>
    <name>A0A673AZF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">trypsin</fullName>
            <ecNumber evidence="10">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115435777</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A673AZF8"/>
    <dbReference type="FunCoup" id="A0A673AZF8">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035974.1">
        <property type="protein sequence ID" value="ENSSORP00005035045.1"/>
        <property type="gene ID" value="ENSSORG00005016501.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZF8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000019">
        <property type="entry name" value="Anionic trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000008">
        <property type="entry name" value="Cationic trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF15">
        <property type="entry name" value="RIKEN CDNA 2210010C04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="44"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="266" mass="29208" checksum="9F6DD54A509C3CC6" modified="2020-06-17" version="1">MQNSDLHVLEDRFCSFKCYFVFVLFCFVLFCFFAYAAPIEDDKIVGGYECRKNSVAYQVSLNSGYHFCGGSLISSTWVVSAAHCYKSRIQVRLGEHNIAVNEGTEQFINSAKVIKHPRYSSRNLDNDIMLIKLSKPASLNSYVRTVSLPSSCASAGTRCLISGWGNTSSSGSYYPDRLRCLDAPILSDSSCRNSYPGQITSNMFCAGFLEGGKDSCQGDSGGPVVCNGQLQGVVSWGYGCAQRNKPGVYAKVCNYNSWIRSTMANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B002</accession>
    <name>A0A673B002_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Microtubule-associated protein RP/EB family member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">EB1 protein family member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">End-binding protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RP3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115415136</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984467.1">
        <property type="nucleotide sequence ID" value="XM_030128607.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029984468.1">
        <property type="nucleotide sequence ID" value="XM_030128608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B002"/>
    <dbReference type="Ensembl" id="ENSSORT00005035642.1">
        <property type="protein sequence ID" value="ENSSORP00005034719.1"/>
        <property type="gene ID" value="ENSSORG00005016365.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415136"/>
    <dbReference type="InParanoid" id="A0A673B002"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000003">
        <property type="entry name" value="microtubule-associated protein RP/EB family member 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000007">
        <property type="entry name" value="Microtubule-associated protein, RP/EB family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="17">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
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        <location>
            <begin position="173"/>
            <end position="241"/>
        </location>
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            <begin position="124"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
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    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="258" mass="29648" checksum="41E6D342BC7878D8" modified="2020-06-17" version="1">MAVNVYSTSMNIENLSRHDMLAWVNDSLQLTFTRIEQLCSGAAYCQFMDMLFPGCILLKKVKFNAKLEHEYIHNFKVLQAAFKRMNVDKIIPVEKLVKGKFQDNFEFLQWFKKFFDANYDGKEYDPLQSRQGQEGTPPPPNPGPMRTSPTAPKSVPAPQRQINAPVRRNTPVTRNGGDAELIELNQQLLDMKLTVDGLEKERDFYFGKLRDIELICQENENNPILGKIMDILYATEEGFAPPEDEDIDEGTRGDQEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B287</accession>
    <name>A0A673B287_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
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        <location>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="809" mass="91785" checksum="4694BEE113F6650A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2A0</accession>
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        <submittedName>
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            <end position="113"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="26694" checksum="20A96B1FEABA7DCA" modified="2020-06-17" version="1">YITLTSLQDPPPHAPYSPFILVSNLRAHLYVALEKNVWMQKRIEELEEERNYLRCQLDRFIISMRSPTEQGMDLFRRSEGMDLFSGSAKEDEEDDSSVETKKKGRGRRNGGEPRMKMRRIFRIMKDPDGVLIRYKKILSTYQRVRSMSRAFQIHGVDRNTMASTSPIAELLLVAPEKVADVGEFDPSKEKLLDYARRCYKTMDEQTHAKVQSMKKTHKLLPISYRFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6T7</accession>
    <name>A0A673B6T7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Solute carrier family 22 member 13a</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Acanthomorphata</taxon>
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            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B6T7"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="222"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="388" mass="43444" checksum="D18DA0B4A032EC63" modified="2020-06-17" version="1">MADFGEILRNIGEFGLFQRVTLVALCFTNFIQGFLLASVLFIEKNPDRHYEQLNLTLPREADETFSKCQMFVPVDWDIDAIREFGLNKTTSCQNGWVYKSTLYKATIVTDFDLVCDKANLLEIIQAISMVGILLGSLLFGPLWIGASKRSWGVCLTLLFTSVGQCALAGLVYYIRDWRLVQIIIAAPLGVVLLYIWCVLFIPESARWLLSRGRTEEAKQFSLNVSFYCLYLNMANLGLNVFLTQFIFGAIEIPAHILCIWLLEVFGRKPLFMLTLATAGVFICLFIYFPGCGIAVTSLAALSRFFLIWAVCVSLLLALFCTISSKTGAIVAPLLNITAVYHWIIPIVVSSGLTFISGGLGFLLHETRRKELPDSTDEAEGNRYIPQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B942</accession>
    <name>A0A673B942_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cdk17</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673B942"/>
    <dbReference type="FunCoup" id="A0A673B942">
        <property type="interactions" value="546"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037946.1">
        <property type="protein sequence ID" value="ENSSORP00005036978.1"/>
        <property type="gene ID" value="ENSSORG00005016443.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B942"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000061">
        <property type="entry name" value="Putative cyclin-dependent kinase 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF128">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="195"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="527" mass="59975" checksum="3FC6661BC83A603F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BA71</accession>
    <name>A0A673BA71_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Lysine-specific demethylase 6B</fullName>
            <ecNumber evidence="16">1.14.11.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">JmjC domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Jumonji domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">[histone H3]-trimethyl-L-lysine(27) demethylase 6B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC115432867</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(27)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60224"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15544"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.68"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with TLE1. Component of the MLL4 complex, at least composed of KMT2B/MLL4, ASH2L, RBBP5, WDR5, and KDM6B. Interacts with TBX21, SMARCA4, SMARCC1 and SMARCC2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the UTX family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.68" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A673BA71"/>
    <dbReference type="Ensembl" id="ENSSORT00005038483.1">
        <property type="protein sequence ID" value="ENSSORP00005037507.1"/>
        <property type="gene ID" value="ENSSORG00005017603.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BA71"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044666">
        <property type="term" value="C:MLL3/4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071558">
        <property type="term" value="F:histone H3K27me2/H3K27me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1370:FF:000001">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.20:FF:000001">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000009">
        <property type="entry name" value="Putative lysine-specific demethylase 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046941">
        <property type="entry name" value="KDM6_GATAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048562">
        <property type="entry name" value="KDM6A_B-like_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048560">
        <property type="entry name" value="KDM6A_B-like_GATAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF27">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(27) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21322">
        <property type="entry name" value="KDM6_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21326">
        <property type="entry name" value="KDM6_GATAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="JmjC" evidence="25">
        <location>
            <begin position="332"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="285"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00059748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00069212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="633" mass="71174" checksum="0CE01C3D89277DF0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BC51</accession>
    <name>A0A673BC51_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">catechol O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tomt</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a catechol + S-adenosyl-L-methionine = a guaiacol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33566"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:134251"/>
            <dbReference type="EC" id="2.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.6" evidence="2"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039198.1">
        <property type="protein sequence ID" value="ENSSORP00005038207.1"/>
        <property type="gene ID" value="ENSSORG00005017877.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BC51"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016206">
        <property type="term" value="F:catechol O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042424">
        <property type="term" value="P:catecholamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042417">
        <property type="term" value="P:dopamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000054">
        <property type="entry name" value="Catechol O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836:SF1">
        <property type="entry name" value="TRANSMEMBRANE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-1009">Hearing</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="265" mass="30418" checksum="14C9B4C4204E8543" modified="2020-06-17" version="1">LPKCPRMVSPAIALAFLPLLLTVIIRYRFYFVLFYRAILIRIWKDCVTGLTREERAFQYVLTHATPGDPESILEAFDEWCSKVEFISNIGPKKGKILDRLLLEQRPLTVLELGTHCGYSTIRIARALPLGARLYSVEMDQRNAAIAEKIIRLAGFDDDTVELIVNPSDEVIPRLRSDYGLERLDFVFMDHWKKCYRPDLELLEGCGLLGKGSMIVADNVLFPGAPKFLRYIRKCGLYEWKIHRATLEYSRGIRDGMAELVYQGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BD73</accession>
    <name>A0A673BD73_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL49</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">39S ribosomal protein L49, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL49 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BD73"/>
    <dbReference type="FunCoup" id="A0A673BD73">
        <property type="interactions" value="1162"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041482.1">
        <property type="protein sequence ID" value="ENSSORP00005040441.1"/>
        <property type="gene ID" value="ENSSORG00005018879.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BD73"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000009">
        <property type="entry name" value="39S ribosomal protein L49, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007740">
        <property type="entry name" value="Ribosomal_mL49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13477">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05046">
        <property type="entry name" value="Img2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="139" mass="15599" checksum="42C655BB2E3394EA" modified="2020-06-17" version="1">MAPVMLRRTLRGALSLPSRIPVAPAGLRFNSAAAPEDKKQVIIESAEEYKFVERLIPPTRVPTPPQHAGPTPSGWTPPADSPPPLPYMIRRSRMYNIPVYTDLTHGNRMMTLVRKVEGDIWQSQPLELSQLSPQEHRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BG64</accession>
    <name>A0A673BG64_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase TRIM21-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115426579</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030000535.1">
        <property type="nucleotide sequence ID" value="XM_030144675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BG64"/>
    <dbReference type="Ensembl" id="ENSSORT00005042603.1">
        <property type="protein sequence ID" value="ENSSORP00005041536.1"/>
        <property type="gene ID" value="ENSSORG00005019316.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426579"/>
    <dbReference type="InParanoid" id="A0A673BG64"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
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        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
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        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="147"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="344"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="543" mass="62273" checksum="5FB6A235A034354B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BI86</accession>
    <name>A0A673BI86_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Vesicle (multivesicular body) trafficking 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VTA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BI86"/>
    <dbReference type="Ensembl" id="ENSSORT00005041417.1">
        <property type="protein sequence ID" value="ENSSORP00005040377.1"/>
        <property type="gene ID" value="ENSSORG00005018854.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BI86"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein VTA1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.270">
        <property type="entry name" value="Vacuolar protein sorting-associated protein vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044538">
        <property type="entry name" value="Vta1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039431">
        <property type="entry name" value="Vta1/CALS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023175">
        <property type="entry name" value="Vta1/CALS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041212">
        <property type="entry name" value="Vta1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46009:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04652">
        <property type="entry name" value="Vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18097">
        <property type="entry name" value="Vta1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vta1/callose synthase N-terminal" evidence="10">
        <location>
            <begin position="18"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Vta1 C-terminal" evidence="11">
        <location>
            <begin position="218"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="259" mass="28287" checksum="5CB6F068A6BB71FF" modified="2020-06-17" version="1">TRMLHTRPPVPAGLFKAIQHHLRTAQEHEKRDPVVAYYCKSAANAEANVDSKSPECRKFLVKLMDQLETVLKSTTSDNDSINQEVVGNAHIENYALKMFLYADNEDRSFYTSSLLFDVLSVLENCLRRKYARWKATYIHTCLKNGDTPQPGPMGLDEDQDQDGGGLGPGPSPGIGFTPGPSGPPSANYNNIQIPPGAHAPANTPAELPPPTGAVQLSPEDFTKAQKYCKYAGSALQYEDVSTAVQNLQKALKLLTTGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BL35</accession>
    <name>A0A673BL35_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115433028</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14 18">Postsynaptic cell membrane</location>
            <topology evidence="14 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030010071.1">
        <property type="nucleotide sequence ID" value="XM_030154211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BL35"/>
    <dbReference type="Ensembl" id="ENSSORT00005042354.1">
        <property type="protein sequence ID" value="ENSSORP00005041292.1"/>
        <property type="gene ID" value="ENSSORG00005019026.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433028"/>
    <dbReference type="InParanoid" id="A0A673BL35"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022824">
        <property type="term" value="F:transmitter-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13715">
        <property type="entry name" value="PBP2_iGluR_AMPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000001">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000004">
        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000666">
        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000013">
        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 18" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027157365" description="Glutamate receptor" evidence="18">
        <location>
            <begin position="22"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="818"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="417"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="20">
        <location>
            <begin position="427"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="679"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="729"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="476"/>
        </location>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="16">
        <location>
            <position position="657"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="684"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="776"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="84"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="742"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="886" mass="100219" checksum="C8CC0E23884D4772" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BN26</accession>
    <name>A0A673BN26_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family with sequence similarity 117 member Ba</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BN26"/>
    <dbReference type="FunCoup" id="A0A673BN26">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042698.1">
        <property type="protein sequence ID" value="ENSSORP00005041628.1"/>
        <property type="gene ID" value="ENSSORG00005019354.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BN26"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026642">
        <property type="entry name" value="Glcci1/FAM117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972">
        <property type="entry name" value="AGAP011572-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972:SF6">
        <property type="entry name" value="PROTEIN FAM117B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15388">
        <property type="entry name" value="FAM117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="227" mass="25103" checksum="48BBD49DDF100D02" modified="2020-06-17" version="1">FRDKLTSTPRAWADERRRGSHKRSASCGSTDQLKEIAKLRQQLQRSKRSSRHRRDKDRKSPFNGSHTIIQSQVINTHSSPMPKTILIPIPISKSSAPRFRNSVEGLNQEIERIIIRDTPEKDEAIVVSPQDVPDGHRAPPPVPPQRSSSTRSIDTQTPSGGGLSGSHSNCSSRPDSISPSYLTVLNDTAGGSPYEDKGEHAIHCTKTQARQSLCGHRHELLRGRSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTK5</accession>
    <name>A0A673BTK5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Interleukin 17a/f1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">il17a/f2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BTK5"/>
    <dbReference type="FunCoup" id="A0A673BTK5">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047340.1">
        <property type="protein sequence ID" value="ENSSORP00005046181.1"/>
        <property type="gene ID" value="ENSSORG00005021165.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BTK5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="14824" checksum="AE70E6C274ED7D06" modified="2020-06-17" version="1">TDSLSPQVCCSALWVVSSSVVKAPPRPGCDSTLAFSSDVLSLSEGGGDIHSRSVSPWRWRSSTVKTRIPSTLWEAECSSSFCSGADTRRTDVLGLNSVPVYQNLLVLTRQEGGDCFNASYRSVAVGCTCVWAKSTPGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVN5</accession>
    <name>A0A673BVN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein lin-52 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lin52</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lin-52 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BVN5"/>
    <dbReference type="FunCoup" id="A0A673BVN5">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048117.1">
        <property type="protein sequence ID" value="ENSSORP00005046946.1"/>
        <property type="gene ID" value="ENSSORG00005021488.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVN5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070176">
        <property type="term" value="C:DRM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018737">
        <property type="entry name" value="DREAM_LIN52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31489">
        <property type="entry name" value="LIN52 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31489:SF2">
        <property type="entry name" value="PROTEIN LIN-52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10044">
        <property type="entry name" value="LIN52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="113" mass="12813" checksum="C167C6A3E0B21C30" modified="2020-06-17" version="1">MTFCIFIGDDFETSLLSFEKLDRASPDLWPEQLPGVAEFAASCKNPITNSPPKWMAELESEDIEMLKELGSLTTANLMEKVKGLQNLAYQLGLEESREMTRGKFLNILERPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BX67</accession>
    <name>A0A673BX67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ino80b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030018645.1">
        <property type="nucleotide sequence ID" value="XM_030162785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BX67"/>
    <dbReference type="Ensembl" id="ENSSORT00005046869.1">
        <property type="protein sequence ID" value="ENSSORP00005045714.1"/>
        <property type="gene ID" value="ENSSORG00005020989.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438897"/>
    <dbReference type="CTD" id="83444"/>
    <dbReference type="InParanoid" id="A0A673BX67"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23021">
        <property type="entry name" value="zf-HIT_IN80B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="210"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="337" mass="37695" checksum="97119C32312C9D9E" modified="2020-06-17" version="1">MGKRKDMIHPRFLSEGSSGLHSVHKHKKQHKKHKKHKRKHHSFPEAPEPEPIVAPRPPPQLRLKIKLGGQTLGTKSVPTFTVHPGVARPPSPLMIIDNDDDDDDEDDDEDDEEPSVPLEQYRAWLDEDSNMATSPMPDLDSDSMLGGPVDEEERWLDALEKGELDDNGELKKEVDESLLTARQKALLHKQQSQPLLELPMGYKEKEMTAEMMQKREERARKRRLQAAKKAEENKNQTIERLTKTSKAKIKSMREKKSKQNQCPMVRYSDSAQGLAISFPAGVSAPAPAPPRPPPPAAVNCGVSGCNNTKKYSCSKTGVPLCSLECYQRNLLLVQGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZS9</accession>
    <name>A0A673BZS9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Arachidonate--CoA ligase</fullName>
            <ecNumber evidence="12">6.2.1.15</ecNumber>
            <ecNumber evidence="13">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">acsl6</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52116"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.15" evidence="12"/>
    <dbReference type="EC" id="6.2.1.3" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A673BZS9"/>
    <dbReference type="Ensembl" id="ENSSORT00005047680.1">
        <property type="protein sequence ID" value="ENSSORP00005046519.1"/>
        <property type="gene ID" value="ENSSORG00005014485.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BZS9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF54">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="18">
        <location>
            <begin position="116"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="694" mass="77616" checksum="7C90C8B184A1D2ED" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C220</accession>
    <name>A0A673C220_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Basic helix-loop-helix family, member e41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">bhlhe41</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029991450.1">
        <property type="nucleotide sequence ID" value="XM_030135590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C220"/>
    <dbReference type="FunCoup" id="A0A673C220">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047557.1">
        <property type="protein sequence ID" value="ENSSORP00005046398.1"/>
        <property type="gene ID" value="ENSSORG00005021242.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420351"/>
    <dbReference type="CTD" id="79365"/>
    <dbReference type="InParanoid" id="A0A673C220"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000020">
        <property type="entry name" value="class E basic helix-loop-helix protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="45"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="13">
        <location>
            <begin position="132"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="205"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="367"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="226"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="392"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="46900" checksum="2F6563F071A28D0C" modified="2020-06-17" version="1">MDERIPHLQDRQFMEHADFLGVDYPSLYMCKSKRGIKREDGGKDAYKLPHRLIEKKRRDRINECIGQLKDLLPEHLKLSTLGHLEKAVVLELTLKHLNALTAVTEQQHQKIIALQNGDRSMKSSIHADLDAFHSGFQACAKEVLQYLSQFENWTTREQRCAQLINHLHKVLAQFQPGAPPIQHQLPTGEDGQKTDSQANCVPVIQRTQGGELNENDTDTDSGYGGEAEKNDGKEKECERNKAQGTKAVKIKQEFGDDRAAKKPKMNWSGNGLGTDPTRPDLAFMNSLMGITNVGQQTPICMPFYFINPTAAASYMPFFDKNNIEKYMYPAAAALASPFPWLYPAHASAAAAAAAAAAFPGLSAHFGASPQSKDSLTPDSEGSHEAETGLPEEREESPASDDGDGDAADASLESQSSPHNPFPACQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C289</accession>
    <name>A0A673C289_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C289"/>
    <dbReference type="FunCoup" id="A0A673C289">
        <property type="interactions" value="2"/>
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    <dbReference type="Ensembl" id="ENSSORT00005047619.1">
        <property type="protein sequence ID" value="ENSSORP00005046458.1"/>
        <property type="gene ID" value="ENSSORG00005021267.1"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Bbox2_xNF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
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        <property type="entry name" value="Butyrophylin_SPRY"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
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    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="160"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="518" mass="59367" checksum="9A053E1ACEA93275" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3I5</accession>
    <name>A0A673C3I5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G protein-activated inward rectifier potassium channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inward rectifier K(+) channel Kir3.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Potassium channel, inwardly rectifying subfamily J member 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C3I5"/>
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        <property type="protein sequence ID" value="ENSSORP00005047809.1"/>
        <property type="gene ID" value="ENSSORG00005021825.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C3I5"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015467">
        <property type="term" value="F:G-protein activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
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        <property type="entry name" value="IRK_C"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
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        <property type="entry name" value="K_chnl_inward-rec_Kir3.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF17">
        <property type="entry name" value="G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01327">
        <property type="entry name" value="KIR31CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 17" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <keyword evidence="7 17" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="19">
        <location>
            <begin position="31"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="20">
        <location>
            <begin position="177"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="16">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="307" mass="35078" checksum="0224636A23BC3245" modified="2020-06-17" version="1">LASLRRNFKHLMLNISVSLPARARRKRQRYVEKNGRCNVQHGNMRETYRYLTDIFTTLVDLNWRCSLFVFVMAYAVTWLFFGAIWYLIAYCRGDLDHLEDEKWTPCVNNVNGFISAFLFSIETETTIGYGHRVITDQCPVGTMLLLLQAILGSMVNAFMVGCMFVKISQPNKRAETLVFSKTAVISLRDDKLCLMFRVGDLRSSHIVGANMRAKLIKSKQTQEGEFIPLDQTDISVGFETGDDRLFLVSPLVISHEIDAHSPFWDMSQAQLEKEDFEIVVILEGMVEATGKKTVKLVCLWNDGNRNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C466</accession>
    <name>A0A673C466_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polymerase (RNA) III (DNA directed) polypeptide D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">polr3d</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029999426.1">
        <property type="nucleotide sequence ID" value="XM_030143566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C466"/>
    <dbReference type="Ensembl" id="ENSSORT00005048266.1">
        <property type="protein sequence ID" value="ENSSORP00005047093.1"/>
        <property type="gene ID" value="ENSSORG00005021552.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425808"/>
    <dbReference type="CTD" id="661"/>
    <dbReference type="InParanoid" id="A0A673C466"/>
    <dbReference type="OrthoDB" id="5836119at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007811">
        <property type="entry name" value="RPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05132">
        <property type="entry name" value="RNA_pol_Rpc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="42504" checksum="D24C4EF012683185" modified="2020-06-17" version="1">MADSGSGDSSGQRVPTPGGSGVGLLSGRRPTAAITAGRLPSMRSRDLTLGGVKKKTFTPNIIGRKVKEETKVEGGQRRDRRDADRGRGPRDRGRGRGRPEVIQSHSIFEQGPAEMMMKKRGVYESERDAPSMGPSPIINIKKEKRETEEETKEILRKLERDNFIDDPFLRSEQRSCPVQVPLAVSGWGFKEEFKVKVEKVEEDIEPMDASVEVKKEPEETEIKAESSFRPPPLPEPEVLPDLLHRWSLSKGEELFFMQLPDSLPGQPPTKEQRPVKTEVQSEDGQSVLLKTESQEVEVEENNCNLKDLREGLIGKMLVRKSGRVQLILGQVTLDVSLGTTCSFLQELVSIGTEGQTGDLTVLGHIKHKMVCSPDFEALLQGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4K1</accession>
    <name>A0A673C4K1_9TELE</name>
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        <recommendedName>
            <fullName evidence="13">Dishevelled segment polarity protein 3a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dvl3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DSH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016181.1">
        <property type="nucleotide sequence ID" value="XM_030160321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C4K1"/>
    <dbReference type="FunCoup" id="A0A673C4K1">
        <property type="interactions" value="1356"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050097.1">
        <property type="protein sequence ID" value="ENSSORP00005048905.1"/>
        <property type="gene ID" value="ENSSORG00005022249.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437165"/>
    <dbReference type="InParanoid" id="A0A673C4K1"/>
    <dbReference type="OrthoDB" id="10031689at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04438">
        <property type="entry name" value="DEP_dishevelled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06717">
        <property type="entry name" value="PDZ_Dishevelled-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.130:FF:000001">
        <property type="entry name" value="Segment polarity protein dishevelled homolog DVL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000040">
        <property type="entry name" value="segment polarity protein dishevelled homolog DVL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000014">
        <property type="entry name" value="Segment polarity protein dishevelled homolog DVL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024580">
        <property type="entry name" value="Dishevelled_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008339">
        <property type="entry name" value="Dishevelled_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003351">
        <property type="entry name" value="Dishevelled_protein_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001158">
        <property type="entry name" value="DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038207">
        <property type="entry name" value="DIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015506">
        <property type="entry name" value="Dsh/Dvl-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10878">
        <property type="entry name" value="SEGMENT POLARITY PROTEIN DISHEVELLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10878:SF6">
        <property type="entry name" value="SEGMENT POLARITY PROTEIN DISHEVELLED HOMOLOG DVL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02377">
        <property type="entry name" value="Dishevelled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00778">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12316">
        <property type="entry name" value="Dsh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01760">
        <property type="entry name" value="DISHEVELLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00021">
        <property type="entry name" value="DAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50841">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="DIX" evidence="10">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="247"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="9">
        <location>
            <begin position="420"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="545"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="598"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="684" mass="74113" checksum="0E05596A53A5183F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6D5</accession>
    <name>A0A673C6D5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cingulin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115421003</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029992514.1">
        <property type="nucleotide sequence ID" value="XM_030136654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C6D5"/>
    <dbReference type="Ensembl" id="ENSSORT00005049001.1">
        <property type="protein sequence ID" value="ENSSORP00005047818.1"/>
        <property type="gene ID" value="ENSSORG00005021827.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421003"/>
    <dbReference type="InParanoid" id="A0A673C6D5"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150105">
        <property type="term" value="P:protein localization to cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF2">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin tail" evidence="4">
        <location>
            <begin position="780"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="129"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="185"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="896"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="984"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="366"/>
            <end position="491"/>
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        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="149"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="896"/>
            <end position="905"/>
        </location>
    </feature>
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        <location>
            <begin position="907"/>
            <end position="917"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1050" mass="121429" checksum="699FFCA54278381C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7D9</accession>
    <name>A0A673C7D9_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">G kinase anchoring protein 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="primary">gkap1</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the GKAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000286.1">
        <property type="nucleotide sequence ID" value="XM_030144426.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051108.1">
        <property type="protein sequence ID" value="ENSSORP00005049905.1"/>
        <property type="gene ID" value="ENSSORG00005022650.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GKAP1"/>
    </dbReference>
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        <property type="entry name" value="G KINASE ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14899:SF0">
        <property type="entry name" value="G KINASE-ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02083">
        <property type="entry name" value="GKINASEAP1"/>
    </dbReference>
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    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="20"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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            <end position="274"/>
        </location>
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        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
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        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
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        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006662"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43157" checksum="D880AAD1D7A94201" modified="2020-06-17" version="1">MASSAMITVPTTASRFALLQIDSDSDSDTSEPGKTSTKSGRESSGKTRQGKAGASGGKAGQGNDKKKDKKKKKREQQQSEANELRNLAFKKIPQKSCAPPQCMTLSGIASELLNPASVDNGIPSEGWQQWKQRDEQMTTELYEADLEKALILSKLEYEQQKENSTNTSSPKSRGGKEGGGKKEKKKSQQAKDKKTVSLQDFQAEGSVDLNKKQDKEEARVANPALGIRQEERFFNKVEDDVNRIIQREKRREQYSNSQGQEVNTSTEHEPDPRAEQLKYELEKKDQEIDKLKKTISQWEVKYKEVKARNAQLLKMLQQGEMKDKAEILLQVEELLHIKEELSAQVTLLHAALEQERSKVKGLQMEQPKHQGNKKGRKGSEMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C985</accession>
    <name>A0A673C985_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Lysosomal beta A mannosidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Mannanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">manba</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.25" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673C985"/>
    <dbReference type="Ensembl" id="ENSSORT00005053395.1">
        <property type="protein sequence ID" value="ENSSORP00005052151.1"/>
        <property type="gene ID" value="ENSSORG00005023522.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C985"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004567">
        <property type="term" value="F:beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000650">
        <property type="entry name" value="Mannosidase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000035">
        <property type="entry name" value="Mannosidase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000060">
        <property type="entry name" value="Probable beta-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054593">
        <property type="entry name" value="Beta-mannosidase-like_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050887">
        <property type="entry name" value="Beta-mannosidase_GH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041625">
        <property type="entry name" value="Beta-mannosidase_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041447">
        <property type="entry name" value="Mannosidase_ig"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43730">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43730:SF1">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22666">
        <property type="entry name" value="Glyco_hydro_2_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17753">
        <property type="entry name" value="Ig_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17786">
        <property type="entry name" value="Mannosidase_ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Beta-mannosidase-like galactose-binding" evidence="20">
        <location>
            <begin position="39"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="17">
        <location>
            <begin position="354"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Mannosidase Ig/CBM-like" evidence="19">
        <location>
            <begin position="712"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-mannosidase Ig-fold" evidence="18">
        <location>
            <begin position="812"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="890" mass="100297" checksum="319554BD8414959A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJ09</accession>
    <name>A0A673CJ09_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fermt2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJ09"/>
    <dbReference type="FunCoup" id="A0A673CJ09">
        <property type="interactions" value="1660"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055452.1">
        <property type="protein sequence ID" value="ENSSORP00005054180.1"/>
        <property type="gene ID" value="ENSSORG00005024132.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJ09"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17181">
        <property type="entry name" value="FERM_F0_KIND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17184">
        <property type="entry name" value="FERM_F1_KIND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01237">
        <property type="entry name" value="PH_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000037">
        <property type="entry name" value="Fermitin family homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000057">
        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000035">
        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
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    </dbReference>
    <dbReference type="InterPro" id="IPR037837">
        <property type="entry name" value="PH_Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF11">
        <property type="entry name" value="FERMITIN FAMILY HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FERM_M"/>
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    </dbReference>
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        <property type="entry name" value="Kindlin_2_N"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="387"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="695" mass="79754" checksum="56EA42886BEDD7FE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJ59</accession>
    <name>A0A673CJ59_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">clip4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029982114.1">
        <property type="nucleotide sequence ID" value="XM_030126254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CJ59"/>
    <dbReference type="Ensembl" id="ENSSORT00005053338.1">
        <property type="protein sequence ID" value="ENSSORP00005052098.1"/>
        <property type="gene ID" value="ENSSORG00005023502.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413426"/>
    <dbReference type="InParanoid" id="A0A673CJ59"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF32">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
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    </dbReference>
    <dbReference type="Pfam" id="PF01302">
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM01052">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="3">
        <location>
            <begin position="305"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="3">
        <location>
            <begin position="520"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="3">
        <location>
            <begin position="672"/>
            <end position="714"/>
        </location>
    </feature>
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        <location>
            <begin position="355"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="599"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="733" mass="78842" checksum="C97DE053ECC20496" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJF9</accession>
    <name>A0A673CJF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BolA-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">bola3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins. Probably acts together with NFU1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with NFU1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029986886.1">
        <property type="nucleotide sequence ID" value="XM_030131026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CJF9"/>
    <dbReference type="FunCoup" id="A0A673CJF9">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053458.1">
        <property type="protein sequence ID" value="ENSSORP00005052213.1"/>
        <property type="gene ID" value="ENSSORG00005023541.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417065"/>
    <dbReference type="CTD" id="388962"/>
    <dbReference type="InParanoid" id="A0A673CJF9"/>
    <dbReference type="OrthoDB" id="203381at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.90:FF:000003">
        <property type="entry name" value="BolA family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="115" mass="12881" checksum="7C441A6EBAB66329" modified="2020-06-17" version="1">MLVCKGSLTNTMLSALRVLRCNQVVFSAQVQRCLSTQTEGEIRIVKILKEKFPLASSLKVVDISGGCGAMYEIHIESSEFKGKRTVQQHQLVNQALKEEIQGMHGLRIFTDFPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMU5</accession>
    <name>A0A673CMU5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear receptor coactivator 4 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ncoa4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030012048.1">
        <property type="nucleotide sequence ID" value="XM_030156188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CMU5"/>
    <dbReference type="FunCoup" id="A0A673CMU5">
        <property type="interactions" value="966"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054593.1">
        <property type="protein sequence ID" value="ENSSORP00005053338.1"/>
        <property type="gene ID" value="ENSSORG00005023978.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434308"/>
    <dbReference type="CTD" id="8031"/>
    <dbReference type="InParanoid" id="A0A673CMU5"/>
    <dbReference type="OrthoDB" id="6334544at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039947">
        <property type="entry name" value="NCoA-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022174">
        <property type="entry name" value="NCOA4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17085">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17085:SF3">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12489">
        <property type="entry name" value="ARA70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear receptor coactivator 4 N-terminal" evidence="2">
        <location>
            <begin position="61"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear receptor coactivator 4 N-terminal" evidence="2">
        <location>
            <begin position="226"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="625" mass="69019" checksum="250A15FCEFC5C2C3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMY6</accession>
    <name>A0A673CMY6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CMY6"/>
    <dbReference type="Ensembl" id="ENSSORT00005054633.1">
        <property type="protein sequence ID" value="ENSSORP00005053378.1"/>
        <property type="gene ID" value="ENSSORG00005023995.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CMY6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="98" mass="10307" checksum="E570FBE6105EBC09" modified="2020-06-17" version="1">MDLSLRDALGGGGGGGVPGGAPAEALLKRDFVATLEKESYDDKVGETVSKSDYRPLLDGKDAKSGKSVHTTVNVTPVVFTCGDEDFQSDTTWIQEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSN9</accession>
    <name>A0A673CSN9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Melanocortin-2 receptor accessory protein 2A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115415033</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MRAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984301.1">
        <property type="nucleotide sequence ID" value="XM_030128441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CSN9"/>
    <dbReference type="FunCoup" id="A0A673CSN9">
        <property type="interactions" value="1116"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056842.1">
        <property type="protein sequence ID" value="ENSSORP00005055557.1"/>
        <property type="gene ID" value="ENSSORG00005024797.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415033"/>
    <dbReference type="InParanoid" id="A0A673CSN9"/>
    <dbReference type="OrthoDB" id="9904651at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031780">
        <property type="term" value="F:corticotropin hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030545">
        <property type="term" value="F:signaling receptor regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070996">
        <property type="term" value="F:type 1 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031781">
        <property type="term" value="F:type 3 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031782">
        <property type="term" value="F:type 4 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031783">
        <property type="term" value="F:type 5 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106070">
        <property type="term" value="P:regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028111">
        <property type="entry name" value="MRAP"/>
    </dbReference>
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        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15183">
        <property type="entry name" value="MRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010063"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="230" mass="26190" checksum="44AE2150DAF8170F" modified="2020-06-17" version="1">MSDFHNRSQTSARRSDYVWQYEYYDDEEPVSFEGLRAHRYSIVIGFWVGLAVFVIFMFFVLTLLTKTGAPHQENPDSAEKRHRPGNCPVDSGSAQDENDKAFSRPLLAESRSYFHFYINEEQAQGKPKLKDKRVGKPQPQGSCKSPGEYSSSGIVEMEEEAEEAEEHQPLKGLLEESKTDGECAFLSHFNIPNFVNLEHSSTLGEDDLLYEPSVILQRQSHSQNGHCDIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSS6</accession>
    <name>A0A673CSS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="23">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">ddx1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="23">The helicase domain is involved in the stimulation of RELA transcriptional activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family. DDX1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="23"/>
    <dbReference type="AlphaFoldDB" id="A0A673CSS6"/>
    <dbReference type="FunCoup" id="A0A673CSS6">
        <property type="interactions" value="2127"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056249.1">
        <property type="protein sequence ID" value="ENSSORP00005054973.1"/>
        <property type="gene ID" value="ENSSORG00005024472.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSS6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="DEADc_DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12873">
        <property type="entry name" value="SPRY_DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000013">
        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000652">
        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000708">
        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent RNA helicase DDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0010">Activator</keyword>
    <keyword evidence="14 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12 23" id="KW-0347">Helicase</keyword>
    <keyword evidence="11 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 23" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="20" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="24">
        <location>
            <begin position="75"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="25">
        <location>
            <begin position="262"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="26">
        <location>
            <begin position="498"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="740" mass="82571" checksum="C1666EE654619388" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CTN3</accession>
    <name>A0A673CTN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Interleukin enhancer binding factor 3b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ilf3b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="858" mass="91867" checksum="D5A49A4B92668CBD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CV92</accession>
    <name>A0A673CV92_9TELE</name>
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            <fullName evidence="14">von Hippel-Lindau disease tumor suppressor</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="13">Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the VHL family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="12"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="166" mass="19326" checksum="F341CC6C026F4E88" modified="2020-06-17" version="1">MPQDGDQPVSLVRSRNSEIPVYAVFCNRSSRVVRPLWINYSGELHPYDDIQPGTGRRMNTFVGHPWIFRDAETDEPMWVNCKELFLPKPAENRHPTIANITLPVYSLKDRALQVIRCLVQPGDYRRLEIARCLHEELEDKPSALKDLHRINQRVEQYLQDATTDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CZW0</accession>
    <name>A0A673CZW0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Apolipoprotein M</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CZW0"/>
    <dbReference type="Ensembl" id="ENSSORT00005059934.1">
        <property type="protein sequence ID" value="ENSSORP00005058607.1"/>
        <property type="gene ID" value="ENSSORG00005025852.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CZW0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11967">
        <property type="entry name" value="ALPHA-1-ACID GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11967:SF2">
        <property type="entry name" value="ALPHA-1-ACID GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025588292" description="Apolipoprotein M" evidence="5">
        <location>
            <begin position="19"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21751" checksum="C8BE246DEC95720F" modified="2020-06-17" version="1">MFATCALVLLCLIPLSCSAPLTCEDLVRPLDHLDPHHLEGRWALVAGGLSDPGLQEFFKRRNSSSINFSSAHAASSFSYTPSVDAGGKCHYKSYNISLEGSVLTFDVLDQVNLTVTFLFTSCPDCLVMRYDNISGKTERVFLFSRRRKVDLDEMEEFRAQAECLKMLPPAVMDHTKELCPEQNSDNHRLLTKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3K2</accession>
    <name>A0A672Y3K2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pou3f3b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y3K2"/>
    <dbReference type="FunCoup" id="A0A672Y3K2">
        <property type="interactions" value="400"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000803.1">
        <property type="protein sequence ID" value="ENSSORP00005000777.1"/>
        <property type="gene ID" value="ENSSORG00005000482.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y3K2"/>
    <dbReference type="OrthoDB" id="6358449at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016362">
        <property type="entry name" value="TF_POU_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF125">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002629">
        <property type="entry name" value="Transcription_factor_POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="14">
        <location>
            <begin position="236"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="326"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="328"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="183"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="436" mass="46574" checksum="E5C706442D4EB92F" modified="2020-06-17" version="1">MATAASNPYLPSNSILSSGSIVHSDSGGGGMQPGSAAVTSGSGGYRGDPSVKMVQSDFMQGAMAASNGGHMLSHAHQWVTSLPHAAAAAAAAAVAAAEAGSPWSSSPVGMTGSPQQQDVKNSARDDLHTGTALHHRPPHLAPHQTHAGAWGSTTAAHINSISGGQQQQQSLIYSQPGGFTVNGMLSPGSQSLVHPGLVRGDTPDLDHGSHHHHHHHQHPHHQHHGGVNSHDPHSDDDTPTSDDLEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSTGSPTSIDKIAAQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEISSLADNLQLEKEVVRVWFCNRRQKEKRMTPPGVAQTPEDVYSQVGNGHFLVDYLKDASEASDQRVTTTSSFHQVILAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3Z5</accession>
    <name>A0A672Y3Z5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y3Z5"/>
    <dbReference type="Ensembl" id="ENSSORT00005000619.1">
        <property type="protein sequence ID" value="ENSSORP00005000593.1"/>
        <property type="gene ID" value="ENSSORG00005000367.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y3Z5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y7X8</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
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    </dbReference>
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    </evidence>
    <sequence length="333" mass="37797" checksum="0F5C92451B0DA184" modified="2020-06-17" version="1">MGGEVQSPDSIRMVLIGKTGCGKSATANTILGNDYFNSRASMRSVTKVCQKTSGEVDGQPVVVVDTPGLFDTNVPNDEIQRELVKCVSMLAPGPHVFLVVMQFSRSTKEEIETVELIKKYFGKKSENFIIVVFTRGDEMEGESLESYMSSGDLEDYIAKLISDCGGRYHVFNNKTQTDRTQVTELMTKIKTMLKRNGSSYYTNDMFQEAEAAIKTEMDRILKEKDAQMKKELDEVDRNIFSAFFFFYLQLCSKLPNIQSFSGFNLLRPSYGFSVHICGQEFHNFIQTEEKKTVHHKGHSIKMLKTASEKTVASSCFQYRHVFNKKKKKKLVLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAR3</accession>
    <name>A0A672YAR3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cathepsin La</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YAR3"/>
    <dbReference type="Ensembl" id="ENSSORT00005000232.1">
        <property type="protein sequence ID" value="ENSSORP00005000215.1"/>
        <property type="gene ID" value="ENSSORG00005000164.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAR3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025644554" evidence="8">
        <location>
            <begin position="24"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="10">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="9">
        <location>
            <begin position="116"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="174"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="298" mass="34070" checksum="F8EAF96FA6DC9E9D" modified="2020-06-17" version="1">MCDKLRHNIVKIALIFLRNFIWTLNWTNTGTCGRPGTPNCTMRPRTDGGEIWEKNLRKIELHNLEQSMGKHSYRLGMNRYGDLSLQEFRQVMRGYRTEARRKTTGSMFMEPNFLNTPKAVDWRQKGYVTPVKDQGNCGSCWAFSATGALEGQRFRKTGKLVSLSEQNLIDCSRPEGNQTTRVWTPRTPTHTREQTTTRGSDEGCGPVGPVSVAIDTRQDTFQFYESGIYYEKNCSSDELDHAVLVVGYGYEGEHEDGRKYWIVKNSWSVHWGQSGYIYMAKDMDNNCGIATEAIYPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBP8</accession>
    <name>A0A672YBP8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Relaxin-3 receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115428772</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YBP8"/>
    <dbReference type="Ensembl" id="ENSSORT00005002257.1">
        <property type="protein sequence ID" value="ENSSORP00005002199.1"/>
        <property type="gene ID" value="ENSSORG00005001339.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YBP8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000248">
        <property type="entry name" value="ATII_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF932">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00241">
        <property type="entry name" value="ANGIOTENSINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="59"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="390" mass="43393" checksum="48C19311BFBDD810" modified="2020-06-17" version="1">MSNPDLKTLEDIISFLNHCSPVSPTNCSIGLEIADDGAPWLGVLISVVYFLVATTGVLGNLLVMFLLYSTHTITTGTINFYVFNLALADLMLSLALPFWAIDIAMDYSWPFGVVACKAVSLLTGLNVFASCFFLTAMSLTRYCFVTTALKPNASLCNRSCTFPITTAFIWTGALVAAVPRAVFADLKYVSDANDTVCLLLFPDGTAWLGINQLLRVLLGFLLPCTTIILSYLLLLRFLCRHKLKGSRSLRKADISKSVAVVVLSFCACWFPYNVITFWSILINLNIVDMSRSYYLAQKYFFPLANCLAFASSCFNPVIYCLIRKEYRVALHNMFLKLNIAIMSKIPYGINYTEPSGQDVPLNNDPNSSRTWEKLTNHANCYTTLNKRKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDA7</accession>
    <name>A0A672YDA7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Neural cell adhesion molecule L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">nfascb</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Growth cone</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YDA7"/>
    <dbReference type="Ensembl" id="ENSSORT00005001885.1">
        <property type="protein sequence ID" value="ENSSORP00005001830.1"/>
        <property type="gene ID" value="ENSSORG00005000257.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDA7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000057">
        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000078">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000114">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000347">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026966">
        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF12">
        <property type="entry name" value="NEUROFASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025330526" description="Neural cell adhesion molecule L1" evidence="19">
        <location>
            <begin position="30"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1023"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="43"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="143"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="252"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="346"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="439"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="529"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="636"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="806"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="914"/>
            <end position="1010"/>
        </location>
    </feature>
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        <location>
            <begin position="1063"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1063"/>
            <end position="1083"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1153" mass="131298" checksum="09CBB2CD74AA460F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDN4</accession>
    <name>A0A672YDN4_9TELE</name>
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        <submittedName>
            <fullName evidence="7">Ribonuclease kappa-B</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="primary">rnasekb</name>
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    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="101" mass="10978" checksum="14CFA00BB5A27507" modified="2020-06-17" version="1">MPSLLFCGPKMAACGIVISIWGVIMLAMLGIFFSAKSAVLIEDVPFTEADIRDDKNPPGNIYSLYNQVGINCFIAAAIYVAVGAVSLCQVRLNKRQEYMVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDQ1</accession>
    <name>A0A672YDQ1_9TELE</name>
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        <submittedName>
            <fullName evidence="9">Complement C1q tumor necrosis factor-related protein 6-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="9" type="primary">c1qtnf6a</name>
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    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YDQ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005001190.1">
        <property type="protein sequence ID" value="ENSSORP00005001156.1"/>
        <property type="gene ID" value="ENSSORG00005000697.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="6138508at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000029">
        <property type="entry name" value="Complement C1q tumor necrosis factor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF52">
        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025368389" evidence="7">
        <location>
            <begin position="17"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="8">
        <location>
            <begin position="106"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="61"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="247" mass="27526" checksum="7F1ABD2F0A2467EC" modified="2020-06-17" version="1">MLGVLVLLSIFSLVASVPPNTTPRPCRRCCDHLPADGSTDPSHSPGLSHVPEVRTYINMTILKGDKGDRGDRGTPGKVGPEGPPGSRGPTGSKGSKGQAGVPGEPCKLQYSAFSVGRRKSLHSLESYQTLVFDTVFVNVESHFNMFEGKFICHIPGIYYFNVNIHTWNFKETYLHIMQNNAEQAIVYAQPADRSIMQSQSVMLQLVLNDEVWIRLYKRERENAVYSDDVDVYITFNGYLIRETVEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YE26</accession>
    <name>A0A672YE26_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD40 repeat-containing protein SMU1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Smu-1 suppressor of mec-8 and unc-52 protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">smu1a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Component of the spliceosome B complex. Interacts with IK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat SMU1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YE26"/>
    <dbReference type="FunCoup" id="A0A672YE26">
        <property type="interactions" value="2053"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001418.1">
        <property type="protein sequence ID" value="ENSSORP00005001376.1"/>
        <property type="gene ID" value="ENSSORG00005000814.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YE26"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000729">
        <property type="entry name" value="SMU1, DNA replication regulator and spliceosomal factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000754">
        <property type="entry name" value="SMU1, DNA replication regulator and spliceosomal factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000082">
        <property type="entry name" value="WD40 repeat-containing protein SMU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045184">
        <property type="entry name" value="SMU1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054532">
        <property type="entry name" value="TPL_SMU1_LisH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17814">
        <property type="entry name" value="LisH_TPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CTLH" evidence="14">
        <location>
            <begin position="40"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="303"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="480"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="513" mass="57600" checksum="32875CFC79B62FAF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHB0</accession>
    <name>A0A672YHB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YHB0"/>
    <dbReference type="Ensembl" id="ENSSORT00005004099.1">
        <property type="protein sequence ID" value="ENSSORP00005003981.1"/>
        <property type="gene ID" value="ENSSORG00005002429.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YHB0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="166" mass="19477" checksum="3AA6E07F982EC413" modified="2020-06-17" version="1">MEEHTNSTQVFFTSTSYVDEKEELVTSTVLPEEIPPIPENQFLVRRSLQSVHKVKNKPPKDLRNKDERQREGIGLPSNFQSTNQRLVMTRSEKGSKSRDLRQYRTPSCSHSRSQDCFWCSETPPHRRQEMQRWRMRAITGEHWIKDDRELAHGKPVMITPGKKSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YI70</accession>
    <name>A0A672YI70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA binding protein fox-1 homolog 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Fox-1 homolog B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">RNA-binding motif protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">RNA-binding protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115423589</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029996331.1">
        <property type="nucleotide sequence ID" value="XM_030140471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YI70"/>
    <dbReference type="Ensembl" id="ENSSORT00005004387.1">
        <property type="protein sequence ID" value="ENSSORP00005004261.1"/>
        <property type="gene ID" value="ENSSORG00005002583.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423589"/>
    <dbReference type="InParanoid" id="A0A672YI70"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12407">
        <property type="entry name" value="RRM_FOX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000004">
        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025670">
        <property type="entry name" value="Fox-1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034237">
        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF31">
        <property type="entry name" value="RNA BINDING PROTEIN FOX-1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12414">
        <property type="entry name" value="Fox-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="207"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="80"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="463" mass="47393" checksum="4D3E650032F49B55" modified="2020-06-17" version="1">MLMSTDVASIMLPVSRGIMDRERDIDTAAGPHASAAGVPAAVRGMKRGDPEPDAQTAATLTDLAGAECKRPRIDGTGTVVGDIGQNNEPLNPGFHGYPPHSQGSQDSTGGQEGLVAPPFTAFPPPPPPQNGLPGTEFGPGAMFGAAGQGTAEIGAGANGASTTATTTNNNGKAEETPQGEAGVQCGTGGAGAGGSAGDSSEAKGTPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENSADAEKAREKLHGTLVEGRKIEVNNATARVMTNKKMVSPYPNGEALSTLPYAGWKLSPMVGAVYGPELYAVPGFPYPSAAAAATTAAAAAFRGAHLRGRGRPVYSTVRAAVPQPAIPAYPGLVFQDSVLSPRLPQGGYPAAAAAAAAYRYAQPAAVTGATAAAAAYSDSYGRVYTTDPYHALAPAAAAYGVGAMASLYRAGYSRFAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YII5</accession>
    <name>A0A672YII5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">B-cell receptor CD22-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115424504</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030141815.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSSORT00005004525.1">
        <property type="protein sequence ID" value="ENSSORP00005004392.1"/>
        <property type="gene ID" value="ENSSORG00005002663.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424504"/>
    <dbReference type="InParanoid" id="A0A672YII5"/>
    <dbReference type="OrthoDB" id="9448246at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025671185" evidence="3">
        <location>
            <begin position="21"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="119"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="215"/>
            <end position="291"/>
        </location>
    </feature>
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        <location>
            <begin position="376"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="402" mass="44759" checksum="D4C8E0469DA34A21" modified="2020-06-17" version="1">MRVGNRNLTWTLMFLSGVICSQWKVEYPQLNICAMKGSSVVIPCSFHHPDNLRPEKVIWGHVKYHRKKAHLIFVGFVKSRFKYIGDSYKNCSLKIHQVDCMDTGKYGIKFIYNNTKRFPAATSSPTLKIKDLKVELKGNKTIKEGGSVNLTCINSCDGEVSSAFVWFKNGEPVNDGAVLYLSNVSHTSSGNYTCSLKTHTRAMSRIVHINVEYSPKNTSASISPSADTDAHITLVCHSHANPPVEAYTWFKSAEGGSVVVGHEPVFSPGDSGRYFCSVTNKHGNQNSSIVTVNIKAFWTTLSKVVLIIATVAVLLPVAAVFIFRRLNSNRAGIVQNTINCPEFTSNQQQDRCACEEETAEIVYTEVYFHTDRNMQQHTASHDDEDNRTVIYSTVSRESPATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIN0</accession>
    <name>A0A672YIN0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YIN0"/>
    <dbReference type="Ensembl" id="ENSSORT00005004535.1">
        <property type="protein sequence ID" value="ENSSORP00005004403.1"/>
        <property type="gene ID" value="ENSSORG00005002668.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YIN0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="General transcription factor IIF subunit 1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="165"/>
            <end position="358"/>
        </location>
    </feature>
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            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
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        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
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            <begin position="270"/>
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        </location>
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            <begin position="324"/>
            <end position="352"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025232"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU366044"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="416" mass="46544" checksum="63A53ED8F719E6CF" modified="2020-06-17" version="1">IFRNTSKKYNIMAFNAGDKVNCSTWTQARMERDMSARRIYGEEETAEGAAGSEFGKKQREEARRKKFGIVTREFKVEDQPWILKVNGKAGKRFKGIKKGGVTENASYYIFTQCPDGAFEAFPVHGWYNFTPLAKHRTLTAEEAEEEWGRRNKVVNHFSIMLQRRLREQERGEEDEDEGEKSGKKKKKGGKGGDLRIHDQDEDLEMSSDDSNSNGESKTKTKKDMGKGKGKKKKKSRSSENEALEDSDDGDYEGQEKKRTPPKRPGGRGSAGSSRTGSRPGTPSIDPASTSNTLRAAASKLEQGKRQNPVGGTDSPAAKRLKMEPNSQSPAPSGKSTPQPPSGKSTPSSSDVQLTEEAVRRYLIRKPMTTKDLLKKFQTKRTGLSNEQTVNVLAQILKRLNPERKNVNDKMHFYLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJR4</accession>
    <name>A0A672YJR4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP binding cassette subfamily A member 2</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YJR4"/>
    <dbReference type="Ensembl" id="ENSSORT00005004955.1">
        <property type="protein sequence ID" value="ENSSORP00005004816.1"/>
        <property type="gene ID" value="ENSSORG00005002914.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJR4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="675"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="718"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="752"/>
            <end position="774"/>
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            <begin position="781"/>
            <end position="802"/>
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    </feature>
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            <begin position="675"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="320"/>
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    </feature>
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            <begin position="327"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="931" mass="104945" checksum="BA9327777739FBDB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKA1</accession>
    <name>A0A672YKA1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cilia- and flagella-associated protein 97</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cfap97</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YKA1"/>
    <dbReference type="Ensembl" id="ENSSORT00005005160.1">
        <property type="protein sequence ID" value="ENSSORP00005005016.1"/>
        <property type="gene ID" value="ENSSORG00005003006.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YKA1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038791">
        <property type="entry name" value="Cfap97/Hemingway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23035">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 97-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="323"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="402"/>
            <end position="448"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
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        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
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        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021424"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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    <evidence type="ECO:0000313" key="4">
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            <dbReference type="Ensembl" id="ENSSORP00005005016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="448" mass="50260" checksum="886CB19EEAA2BD43" modified="2020-06-17" version="1">MFNPSELEGEIDHSFFDSDCDDVISRDGGKKMVKNSKTEENPPVQEKLQTKQTENAKDGVHLSGKETKKYLKPVKNDNSRGAGLKDISCQPKEKDRDRSFGTSTDSKSDQNFNHRDSGEEHNFYRGKFLNLLSREEDRNEDESSNETEEETSHAKCSSSKLKNVRSPKKLTRNRRSRTPYPDGSYSSSSGRSSVASPTLPRPDNPLSIKGRGTGVGLAASQDLRPLHTDSEDTVTDVSPLSSPDSSRLQSLDLNHTETEDGTVKEQEQESMPSSGLNSLDSESQFGDRVVFHCPVGKNRKNYSFTNDEVRRIDRENQRLLGQLSRLYQGSRPGSAAGQKRHKTSNSPLTRRSHSALNRQREQQRIERENLAFLKRLETVKPTSGLKRSEQLADYQRQVGYLGGPSYPIFKSTTKKERPTSRTSSGYKDPSDVPVPRTKITTGSRPAWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKV1</accession>
    <name>A0A672YKV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 23">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">hpdb</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Catalyzes the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, one of the steps in tyrosine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>3-(4-hydroxyphenyl)pyruvate + O2 = homogentisate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16189"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="EC" id="1.13.11.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:16190"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="24">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 23">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YKV1"/>
    <dbReference type="Ensembl" id="ENSSORT00005005396.1">
        <property type="protein sequence ID" value="ENSSORP00005005240.1"/>
        <property type="gene ID" value="ENSSORG00005003076.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YKV1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000008">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000022">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF12">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17 24" id="KW-0408">Iron</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="25" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025331343" description="4-hydroxyphenylpyruvate dioxygenase" evidence="25">
        <location>
            <begin position="27"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Glyoxalase/fosfomycin resistance/dioxygenase" evidence="26">
        <location>
            <begin position="40"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Glyoxalase/fosfomycin resistance/dioxygenase" evidence="26">
        <location>
            <begin position="202"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00033727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRNR" id="PIRNR009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="414" mass="47448" checksum="48B7EC636917F994" modified="2020-06-17" version="1">MYSRWIALLVVTFFFFFAFRLQTTYTDKGEKHDQGRFLCFDHLTFWVGNAKQAASYYCNKLGFEPLAYQGLETGSRDVVSHAVKQGKIVYVFSSALNPGNKAMGEHLVKHGDGVKDIAFTVEDCDYLVQKARERGAIILKEPFTLEDKFGRVRLAVLQTYGDTTHTFVERTGYKGLFLPGFHPPLFKDPLLNKLPSGKLNFIDHVVGNQPDDEMVPVVEWYQRNLLFHRFWSVDDKQLQTEFSALRSIVVANYEETVKMPINEPAMGKRKSQIQEYVEYYGGPGVQHIAMNTSDIITAIRNLKERGMEFMSVPDTYYEQLRENLKHSKVKVEEDLDTLQELKILVDYDDNGYLLQIFTKPVQDRPTVFLEVIQRHNHQGFGAGNFKSLFEAIEADQHARGNLTILTPNGVSKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLQ1</accession>
    <name>A0A672YLQ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Arf-GAP with dual PH domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115423962</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029996866.1">
        <property type="nucleotide sequence ID" value="XM_030141006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YLQ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005005712.1">
        <property type="protein sequence ID" value="ENSSORP00005005525.1"/>
        <property type="gene ID" value="ENSSORG00005003212.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423962"/>
    <dbReference type="InParanoid" id="A0A672YLQ1"/>
    <dbReference type="OrthoDB" id="73919at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08832">
        <property type="entry name" value="ArfGap_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13252">
        <property type="entry name" value="PH1_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01251">
        <property type="entry name" value="PH2_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000011">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000080">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000099">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052589">
        <property type="entry name" value="Arf-GAP_dual-PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037849">
        <property type="entry name" value="PH1_ADAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037851">
        <property type="entry name" value="PH2_ADAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021:SF5">
        <property type="entry name" value="ARF-GAP WITH DUAL PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021">
        <property type="entry name" value="ARF-GAP WITH DUAL PH DOMAIN-CONTAINING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="10">
        <location>
            <begin position="9"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="132"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="255"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="377" mass="43655" checksum="E4D42368088A6142" modified="2020-06-17" version="1">MASTQDKNRQRLRQLLNEPGNGTCADCGAADPEWASYTLGVFVCHSCSGLHRNIAQISKVKSVHLDPWSTSEVEFMNSVGNNAAKAKHEQIVPAFYYRPTHKDCTLLREQWIRAKYERKEFVCVERQEPYSAGYREGFLWKRGRDNGQYLSRKFILSEREGVLKYFNKHDAREPKAIMKINTVNASFQPTKIGTAHGLQITYLKDNSTRNIFVYHEDGKEMVDWFNAIRAARFHYLQVAFPGAPNTDLLPKLTRNYIKEGYMEKTGPKHTEGFKKRWFTMDDRRLMYFKDPLDAYARGEVFIGSKESGYIVLSGLPTSIQGNHWQFGITIVTPDRKFLFACETEEDQKDWIAAFQTVINRPMLPQEYAVEAYFKHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMZ8</accession>
    <name>A0A672YMZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ASH1 like histone lysine methyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YMZ8"/>
    <dbReference type="Ensembl" id="ENSSORT00005006236.1">
        <property type="protein sequence ID" value="ENSSORP00005005986.1"/>
        <property type="gene ID" value="ENSSORG00005003571.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YMZ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147:SF2">
        <property type="entry name" value="SET-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="26620" checksum="72F076C76A1CDB4A" modified="2020-06-17" version="1">MDQRVKGGTPPTINSTVDPSSAPEDSEKDQVAENRKKGEGENGELGKKEEEKRGVGRKREGDKGAVLELLIEGRCGSTGQQQLQISGRETSCPEGNVRLRIGLQAKRTKKPPKILESYVCKPTIRTYQRQGRGGLVKGEAEGGVGQQSKTSSTPDEASREQRSGLDAVQTTSKQTASAAPPPLASSSSLSSSSSSSSQTLSLYSTFTTSTPASVTALSSQETKTAKQVRTRALCLTCTDSSTSSANNKPVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPB0</accession>
    <name>A0A672YPB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium channel, voltage-gated, type II, beta</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPB0"/>
    <dbReference type="FunCoup" id="A0A672YPB0">
        <property type="interactions" value="1004"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006676.1">
        <property type="protein sequence ID" value="ENSSORP00005006419.1"/>
        <property type="gene ID" value="ENSSORG00005003783.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPB0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000920">
        <property type="entry name" value="Myelin_P0-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869">
        <property type="entry name" value="MYELIN P0 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869:SF3">
        <property type="entry name" value="SODIUM CHANNEL SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00213">
        <property type="entry name" value="MYELINP0"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025650109" evidence="11">
        <location>
            <begin position="29"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="208" mass="23280" checksum="609FFB40E2FEFC05" modified="2020-06-17" version="1">NKKMFKKIVAHNFCVLFTVLLVDFCCSCMDVLVPANINALNGTNVKIQCTFTSCYKMDATRFAMNWTYQETLNDTEEMVMLLVSTHRFGDRVTFTGNLDKNDLSVTLSDVQLEDEGIYNCYVRNPPDRFQGHGVIRLNVLTELPPPSRHSTIAVAIGASVGGALALLILSMVVVKCLRRHRKQELISEEKMEEEGKLEAEGVAEEGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPP7</accession>
    <name>A0A672YPP7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RISC-loading complex subunit TARBP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tarbp2</name>
        <name evidence="8" type="synonym">TARBP2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Self-associates. Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRKRA family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TARBP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989776.1">
        <property type="nucleotide sequence ID" value="XM_030133916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YPP7"/>
    <dbReference type="FunCoup" id="A0A672YPP7">
        <property type="interactions" value="909"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006836.1">
        <property type="protein sequence ID" value="ENSSORP00005006576.1"/>
        <property type="gene ID" value="ENSSORG00005003856.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419253"/>
    <dbReference type="CTD" id="6895"/>
    <dbReference type="InParanoid" id="A0A672YPP7"/>
    <dbReference type="OrthoDB" id="10056847at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070883">
        <property type="term" value="F:pre-miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098795">
        <property type="term" value="P:global gene silencing by mRNA cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903798">
        <property type="term" value="P:regulation of miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070921">
        <property type="term" value="P:regulation of siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046782">
        <property type="term" value="P:regulation of viral transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070922">
        <property type="term" value="P:RISC complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10844">
        <property type="entry name" value="DSRM_TARBP2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19893">
        <property type="entry name" value="DSRM_TARBP2_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000005">
        <property type="entry name" value="Putative double-stranded RNA-specific adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000019">
        <property type="entry name" value="RISC-loading complex subunit TARBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000018">
        <property type="entry name" value="RISC-loading complex subunit TARBP2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03034">
        <property type="entry name" value="TRBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051247">
        <property type="entry name" value="RLC_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028605">
        <property type="entry name" value="TRBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044470">
        <property type="entry name" value="TRBP2_DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044471">
        <property type="entry name" value="TRBP2_DSRM_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205:SF1">
        <property type="entry name" value="RISC-LOADING COMPLEX SUBUNIT TARBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="5 8" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="DRBM" evidence="10">
        <location>
            <begin position="30"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="10">
        <location>
            <begin position="140"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="10">
        <location>
            <begin position="272"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="345" mass="37052" checksum="AFE488332600D63A" modified="2020-06-17" version="1">MNDETASDSWKRNSGCSSIEQMLAVNPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVSVGEINCTGQGPSKKAAKHKAAEAALKMLKGGLGGPAGLGVGVDGFIGVDVPADGDSSQPDMKTLSSSQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFVEIGSGTSKKLAKRNAAAKMLSRIHDVPVDLRTSNDADTEDDTFTMHMGSRAESGKSKGFSCTWDSLRNSAGEKILQLRSHPLGIPSDSNFCSLLTDLSVEQRFDVSYLDLEERSLSGLCQCLVELSTQPITVCHGFAPSIDAARANAAHNALQYLKIMAGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUF8</accession>
    <name>A0A672YUF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115416761</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.</text>
    </comment>
    <comment type="function">
        <text evidence="5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="RefSeq" id="XP_029986481.1">
        <property type="nucleotide sequence ID" value="XM_030130621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YUF8"/>
    <dbReference type="FunCoup" id="A0A672YUF8">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008500.1">
        <property type="protein sequence ID" value="ENSSORP00005008213.1"/>
        <property type="gene ID" value="ENSSORG00005004577.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416761"/>
    <dbReference type="InParanoid" id="A0A672YUF8"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000001">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF11">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025711588" description="Peptidyl-prolyl cis-trans isomerase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="32"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="203" mass="21624" checksum="8504097CA603B09B" modified="2020-06-17" version="1">MLLLPAALLLCAAAPVSAAASRTGPKVTDKVFFDITVAGHEVGRIVIGLFGEVVPLTVNNFVALATGEKGYGYKGTKFHRVIKDFMIQGGDFTAGDGTGGHSIYGTTFADENFKLKHLGAGWVSMANAGPDTNGSQFFILAARAPWLDGKHVVFGKVLDGMSVVHTIELQDTNDRNLPNTECVIVNSGRIAVKEPFMVEVEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YVG3</accession>
    <name>A0A672YVG3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mast cell protease 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115418186</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029988435.1">
        <property type="nucleotide sequence ID" value="XM_030132575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YVG3"/>
    <dbReference type="FunCoup" id="A0A672YVG3">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008759.1">
        <property type="protein sequence ID" value="ENSSORP00005008462.1"/>
        <property type="gene ID" value="ENSSORG00005004701.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418186"/>
    <dbReference type="InParanoid" id="A0A672YVG3"/>
    <dbReference type="OrthoDB" id="5597713at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Kallikrein related peptidase 11"/>
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    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase_S1A"/>
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        <property type="entry name" value="Trypsin_dom"/>
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        <property type="entry name" value="TRYPSIN_HIS"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SERINE PROTEASE 57"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025525920" evidence="6">
        <location>
            <begin position="19"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="21"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="244" mass="26550" checksum="CE087671FC1B1FE5" modified="2020-06-17" version="1">MAVSFAILLFFVLYGADGSLIVGGRDAAPHSRPYMASLQVRGRHVCGGLLVKEDFVLTAAHCELPVPFTVVLGADSLTANETAKQTLNSVRFIPHPDYDGHQNDIMLLKLNRQAQLTEEVQLISLQRNRLTSASQCITAGWGDIGDNNTIANRLQEVNVTILQQRTCRRRWRGVPITRTMVCGTGARAFQGFCSGDSGGPLVCNGAAAGVVSFSGRRCGDPVTPDVYTRISSFREWITNILNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWF2</accession>
    <name>A0A672YWF2_9TELE</name>
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        <recommendedName>
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        </recommendedName>
    </protein>
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        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSSORT00005009063.1">
        <property type="protein sequence ID" value="ENSSORP00005008762.1"/>
        <property type="gene ID" value="ENSSORG00005004838.1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="96" mass="10706" checksum="D28234DD8654E18B" modified="2020-06-17" version="1">MLLTVSMCPPSDDPVVKPVVGVYRAASTALDGKISLMCVASGMFPPEVRFSWKRQKDNGTEEDLPHAQGEQLEVQESGRRTSIRTIHQNHNSSYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXK2</accession>
    <name>A0A672YXK2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA methyltransferase 1-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dmap1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029987967.1">
        <property type="nucleotide sequence ID" value="XM_030132107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YXK2"/>
    <dbReference type="Ensembl" id="ENSSORT00005009656.1">
        <property type="protein sequence ID" value="ENSSORP00005009348.1"/>
        <property type="gene ID" value="ENSSORG00005005122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417895"/>
    <dbReference type="CTD" id="55929"/>
    <dbReference type="InParanoid" id="A0A672YXK2"/>
    <dbReference type="OrthoDB" id="19740at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transcription corepressor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11658">
        <property type="entry name" value="SANT_DMAP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000087">
        <property type="entry name" value="DNA methyltransferase 1-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032563">
        <property type="entry name" value="DAMP1_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008468">
        <property type="entry name" value="DMAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027109">
        <property type="entry name" value="Swc4/Dmap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855:SF10">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12855">
        <property type="entry name" value="DNA METHYLTRANSFERASE 1-ASSOCIATED PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05499">
        <property type="entry name" value="DMAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16282">
        <property type="entry name" value="SANT_DAMP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DAMP1 SANT/Myb-like" evidence="9">
        <location>
            <begin position="121"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="DNA methyltransferase 1-associated 1" evidence="8">
        <location>
            <begin position="240"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="408"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="484" mass="55237" checksum="786E54A08374DA81" modified="2020-06-17" version="1">MATGADVRDILELTGAENDGPISKKDLINSDKKKTKKATETLTFKRPEGMHREVYALLYSDKKDAPPLLPSDTTQGYRTVKAKLGCKKVRPWKWMPFTNPARRDGAIFHHWRRVAEEGKDYPFARFNKTVQVPVYSEQEYQMHLHDDGWTKAETDHLFDLCKRFDLRFIVVHDRYDHQQYRKRSVEDLKERYYNICGKLTKVRAASGTEPKIYVFDAGHERRRKEQLEKLFNRTPEQVAEEEYLIQELRKIETRKKEREKKTQDIQKLIKAADTTTELRRAEKRVSKKKLPQKREMEKPAVPETAGIKFPDCKSAGVALRSHRMKLPSSVGQKKIKAIEQILIEQGVDLNPMPTEEIVQMFNELRSDLVLLYELKQAHSNCEYEQQMLRHRYEALLKAGGGTGCGGTTGTGPVATTQAGDLNPTNNTTASTPGGDAQAWPSADDIKVESKEQIIDVVGAPLTPNSRKRRESASSSSSVKKVKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZ40</accession>
    <name>A0A672YZ40_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SMB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZ40"/>
    <dbReference type="Ensembl" id="ENSSORT00005009981.1">
        <property type="protein sequence ID" value="ENSSORP00005009657.1"/>
        <property type="gene ID" value="ENSSORG00005005263.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZ40"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.20:FF:000001">
        <property type="entry name" value="Ectonucleotide pyrophosphatase/phosphodiesterase family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF107">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="SMB" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="7">
        <location>
            <begin position="34"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="253" mass="28613" checksum="6767D1D4482EF76C" modified="2020-06-17" version="1">PFDGDVPGCRCDVNCNVTDSCCYDYHDTCTVPTQQWECTKLRCGEKRLSQSRCHCSDDCLSAGDCCTNYKHVCHGEPQWVEDECDDLSTPTCPDGFSRQPLLLISLDGLRAEYLQTWSHLIPVLHKLKTCGTSAPYMQAAFPSKTFPNHYTIVTGLYPESNGLIDNSMYDPVMDASFSLSSPEKDNPAWYLGQPIWHTAKHQGLKSGTFFWPGSDVKINGSFPDIYRPYNGKIPFEERVLTVLKWLQLPHDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZT1</accession>
    <name>A0A672YZT1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZT1"/>
    <dbReference type="Ensembl" id="ENSSORT00005010324.1">
        <property type="protein sequence ID" value="ENSSORP00005009994.1"/>
        <property type="gene ID" value="ENSSORG00005005443.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZT1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF5">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="14">
        <location>
            <begin position="150"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="13">
        <location>
            <begin position="322"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="47722" checksum="C3DAD2A089C4935E" modified="2025-10-08" version="2">MFTPIISCYIFFFHPFLPLSHPESVLCDVGEFHCHTKETCIPESWLCDGEPDCPDASDETDQTCAQEVVIRCPLNHIQCIGTKKCIHFNKLCNGARDCDDGYDEGVHCRELLSACHELRCRYGCVMTRNGTFCFCADGFEVGEDGTSCRDHDECAMYGACSQTCTNTYGSYRCSCTQGYILQPDRISCKAKQDPGDPRPVLLIGGPDRIVITDLNGTGLQPLRSLSVNGTLALDFQHNQEHVCWVLSTESSGQLRCAETRSLRGFTREREIRTQQSLQHVEQMAIDWLTGNFYFVDRVTDKIFVCDRSGNTLFCNLHNTLIQMLFFTDYGNVAKVERCNMDGTNRTRLVDYNIEQPTAVALDVVKKLLYWADAYLDYIDVVDYNGRNRHTIIHGSQVSVTFHWEGRGYFFWFGLFVLLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z085</accession>
    <name>A0A672Z085_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Myosin regulatory light chain 2, smooth muscle minor isoform-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">myl12.2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z085"/>
    <dbReference type="Ensembl" id="ENSSORT00005010613.1">
        <property type="protein sequence ID" value="ENSSORP00005010280.1"/>
        <property type="gene ID" value="ENSSORG00005005567.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z085"/>
    <dbReference type="OrthoDB" id="429467at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000010">
        <property type="entry name" value="Myosin regulatory light chain 2, atrial isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000007">
        <property type="entry name" value="Putative myosin regulatory light chain sqh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="172" mass="19814" checksum="9CBF49CB2B62F816" modified="2020-06-17" version="1">MSSKRAKGKNTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEIMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEGTGIIQEEYLRELLTTMGDRFTDEEVDELFREAPIDKKGNFNYVAFTRILKHGAKDKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z367</accession>
    <name>A0A672Z367_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z367"/>
    <dbReference type="Ensembl" id="ENSSORT00005011361.1">
        <property type="protein sequence ID" value="ENSSORP00005010987.1"/>
        <property type="gene ID" value="ENSSORG00005005940.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z367"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025246">
        <property type="entry name" value="IS30-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF129">
        <property type="entry name" value="TRANSPOSABLE ELEMENT TC3 TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13936">
        <property type="entry name" value="HTH_38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase IS30-like HTH" evidence="3">
        <location>
            <begin position="3"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="68"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="181"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="32027" checksum="2BD101EC9D73640B" modified="2020-06-17" version="1">MAKAKKLSLLERGRIVQLHKQGLWQRAIAAEVGRSKTVILNFLTDPEHYGTKKSSGRPKKITPALNWRIRLTVRQDTGRSSTQIKALTGADCSAITIRRHLREKGFKNKKRIQRPRLLQRHKTARLDFAREYQTWDTERWKKVLFSDEKKFNLDGPDGFQRYWHDKEIPPEMFSTHTVEGVHNARLTKDFFRENNITLLDHPACSPDLNPIENIWGWMAREVYKNGHQFHTVDALHEAIFTTWSNVPTSLLETFASTMPKRIFEVINKNGGATHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3J1</accession>
    <name>A0A672Z3J1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z3J1"/>
    <dbReference type="Ensembl" id="ENSSORT00005011751.1">
        <property type="protein sequence ID" value="ENSSORP00005011370.1"/>
        <property type="gene ID" value="ENSSORG00005006094.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3J1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF3">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 1 ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="21"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="120" mass="13276" checksum="7F89B05B49F2B38B" modified="2020-06-17" version="1">YLGNDNKPEISYADLKALLGKSQNLLLIDVRTKEEVDKGRIPGSIHIPVNTVEEALAMEPEEFKSKYGVTKPPLDTPELVFHCQMGKRGATATSKALEAGYLNACNYAGGYKEWFEKEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5H3</accession>
    <name>A0A672Z5H3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">IRF tryptophan pentad repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">irf5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030005443.1">
        <property type="nucleotide sequence ID" value="XM_030149583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z5H3"/>
    <dbReference type="FunCoup" id="A0A672Z5H3">
        <property type="interactions" value="913"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012943.1">
        <property type="protein sequence ID" value="ENSSORP00005012546.1"/>
        <property type="gene ID" value="ENSSORG00005006583.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429834"/>
    <dbReference type="CTD" id="3663"/>
    <dbReference type="InParanoid" id="A0A672Z5H3"/>
    <dbReference type="OrthoDB" id="9856880at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000003">
        <property type="entry name" value="Interferon regulatory factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000093">
        <property type="entry name" value="Putative interferon regulatory factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
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    <dbReference type="InterPro" id="IPR019471">
        <property type="entry name" value="Interferon_reg_factor-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10401">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01243">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="9">
        <location>
            <begin position="8"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="485" mass="55050" checksum="3F291AAB8B490994" modified="2020-06-17" version="1">MTSQQPRRVRLKPWLLAQVSSGRYPGLQWLSPECRLFQIPWKHATRHLPVSEEENTIFKAWALETGKYQEGVDEPDPAKWKANLRCALNKSREFQLKYDGTKDTPVKPYKIYEVCDTIGCSDAVDDDEEEEMPNLMELSINPRLSDPQSFSSFPIPSEGDPFSLSANGTLPHIIPMPLLSDAMHTNPTVTSDLPRQEFQAAGHMVDQGAFAGLQDLNAMPPPPVMEARSMEGIQTQTDPQTQTCKYDLLSSVPLTDLDLKFVYRGRTMGSLTVSNPQGCRLYYGNLEPTPEQVDLFGPVNLQQVLFPGTDQIQNQKQRFYTGALLDVMDRGLILEIWEQDIYAIRLCQCKVFWSGPGVPEQALPNPIEREKKIKVFSLNDFLQGLILFQRGETENPPPFEITFCFGEDWPDRKPKEKKLIIVQVVPVVARILTEMFSGELSWSTDSIRLQISNPDVKDQTVEQFKELQRLLQNQHNQGPWTPSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7G7</accession>
    <name>A0A672Z7G7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein Nkx-3.1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nkx3-1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z7G7"/>
    <dbReference type="Ensembl" id="ENSSORT00005013206.1">
        <property type="protein sequence ID" value="ENSSORP00005012804.1"/>
        <property type="gene ID" value="ENSSORG00005006693.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z7G7"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF38">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX-3.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="87"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="89"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="199" mass="23324" checksum="5504617A0BEBDF63" modified="2020-06-17" version="1">MDRTDMSDQAKPLTSFLIEDILSIKDSTRFNGKWCSQKMERCSQWKEESEQLVEQLSPPGTAFEVQTESLDTSCPSSSESTTFTSRGKQKRSRAAFTHLQVLELEKKFNHQKYLSAPERAHLANTLRLTETQVKIWFQNRRYKTKRKQQTEICKDVYKTDGLALRDGLVRSSLFTSFYKTYQYSPYLWDYSGPWGQTLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCN6</accession>
    <name>A0A672ZCN6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">wipf2a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZCN6"/>
    <dbReference type="Ensembl" id="ENSSORT00005015053.1">
        <property type="protein sequence ID" value="ENSSORP00005014624.1"/>
        <property type="gene ID" value="ENSSORG00005007472.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZCN6"/>
    <dbReference type="OrthoDB" id="5877983at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22077">
        <property type="entry name" value="WH2_WAS_WASL-2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053099">
        <property type="entry name" value="WAS/WASL-interacting_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48226">
        <property type="entry name" value="OS06G0326200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48226:SF1">
        <property type="entry name" value="WAS_WASL-INTERACTING PROTEIN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
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    </evidence>
    <sequence length="950" mass="106916" checksum="01C951E5EA42E0DD" modified="2020-06-17" version="1"> </sequence>
</entry>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="54"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="304"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="74"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="378" mass="42596" checksum="A0D56DA152F102EE" modified="2020-06-17" version="1">MEFTTLRFDAVGDYNEHYAVSLTPFNVTFTEDPMLLHSKSNDTNRAATRDTSSLIIAICITALYSLICVVGLLGNVLVMYGVVRYTKMKTATNIYIFNLALADALATSTLPFQSAKYLMGTWPFGELLCKVVMAIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPAKAKLINVCIWILSSAVGVPVMVMAVTNMTDEGKTVCTLRFPKPEKYWDTVTKIFVFIFAFVVPVLVITICYGLMILRLKSVRLLSGSKEKDRNLRRITRMVLVVVAAFVICWTPIHIFIIVRTMVNTDKNNLVVVASWHLCIALGYTNSSLNPVLYAFLDENFKRCLRDFCLPYHSRMEQNSFSRARNSAREPASVCAPAMRETAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZSE9</accession>
    <name>A0A672ZSE9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein kinase NIM1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">NIM1 serine/threonine-protein kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZSE9"/>
    <dbReference type="Ensembl" id="ENSSORT00005019865.1">
        <property type="protein sequence ID" value="ENSSORP00005019307.1"/>
        <property type="gene ID" value="ENSSORG00005009492.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZSE9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000346">
        <property type="entry name" value="Serine/threonine-protein kinase NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF105">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NIM1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="40"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="403" mass="45601" checksum="41AFF60AA1FC26BF" modified="2020-06-17" version="1">AVVLQSERMNQSPFERVIYDMAHNERIANELILGRRVGFYELRGEIGQGNFSTVRLGIHALTKERVAVKIMDKQRLEKKNQLMTSSEISCMEKLGHPNIVRLYEVIETSRKLYLVMEYGSSGDLFSRITTRGKLNDLDAKMIFAQIISAVKHMHENNIIHRDLKAENVFCTTSYCIKVGDFGFSTECGPNDLLTNFCGSPPYAAPELFKDKGYNGRYSDIWALGILLYFMVTATMPFYGDNMGRLKRCILQGAFSIPAYVPDHCQLVIKSILRPVPVDRLCLTEIVKSAWLSGIEYTKPHVRLPLSPAHFAQADKALCIEEQEVKDLLSDLGIVTVHLQNNPCSDCRSPLTGTYRILLHRVQKRRSVEAVGYSALHPDDYKNQKTWSEAPVEKHNPSAVCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZT41</accession>
    <name>A0A672ZT41_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histone-lysine N-methyltransferase EHMT1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ehmt1a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZT41"/>
    <dbReference type="FunCoup" id="A0A672ZT41">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020902.1">
        <property type="protein sequence ID" value="ENSSORP00005020331.1"/>
        <property type="gene ID" value="ENSSORG00005006679.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZT41"/>
    <dbReference type="OrthoDB" id="5792673at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046974">
        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20905">
        <property type="entry name" value="EHMT_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000005">
        <property type="entry name" value="Euchromatic histone-lysine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043550">
        <property type="entry name" value="EHMT1/EHMT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047762">
        <property type="entry name" value="EHMT_CRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46307">
        <property type="entry name" value="G9A, ISOFORM B"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46307:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EHMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21533">
        <property type="entry name" value="EHMT1-2_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="530"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="563"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="630"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="696"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="9">
        <location>
            <begin position="818"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="884"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="405"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1028" mass="112527" checksum="46EA2C681BE9EE2A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTD2</accession>
    <name>A0A672ZTD2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Paired box 7b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115423164</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZTD2"/>
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    </dbReference>
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        <property type="gene ID" value="ENSSORG00005009415.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZTD2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00131">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000080">
        <property type="entry name" value="paired box protein Pax-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000035">
        <property type="entry name" value="paired box protein Pax-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000031">
        <property type="entry name" value="Paired box protein Pax-7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="HD"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022106">
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    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF45">
        <property type="entry name" value="PAIRED BOX 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12360">
        <property type="entry name" value="Pax7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0563">Paired box</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="14">
        <location>
            <begin position="34"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="220"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="222"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="170"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="312"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="170"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="56650" checksum="D1374E22445C1C15" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUD0</accession>
    <name>A0A672ZUD0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZUD0"/>
    <dbReference type="Ensembl" id="ENSSORT00005021405.1">
        <property type="protein sequence ID" value="ENSSORP00005020780.1"/>
        <property type="gene ID" value="ENSSORG00005010140.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021406.1">
        <property type="protein sequence ID" value="ENSSORP00005020781.1"/>
        <property type="gene ID" value="ENSSORG00005010142.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="12"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="82" mass="9363" checksum="F2FEB6D0531E931D" modified="2020-06-17" version="1">MTEGPGLCGNSWVFQQDNAAVHNARLTKDFFRENNITLLDHPACSPDLNPIENIWGWMAREVYKNGHQFQTVDALREAIFTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A399</accession>
    <name>A0A673A399_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dysbindin domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dysbindin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A399"/>
    <dbReference type="FunCoup" id="A0A673A399">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023775.1">
        <property type="protein sequence ID" value="ENSSORP00005023103.1"/>
        <property type="gene ID" value="ENSSORG00005011189.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A399"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007531">
        <property type="entry name" value="Dysbindin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16294:SF4">
        <property type="entry name" value="DYSBINDIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16294">
        <property type="entry name" value="DYSTROBREVIN BINDING PROTEIN 1 DYSBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04440">
        <property type="entry name" value="Dysbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="154" mass="16969" checksum="A4F500FBF69B1FD3" modified="2020-06-17" version="1">TIASGGIKNSPNKDIQKLLKPSSSGDLSRDLFQHPAGEEEGGLSGHTASLLHITEKRQPLSSVSSLEVHFDLLDLTELTDMSDQELAEVFADSDEENHNESPAGSHQSVLPKGGYMRSPSWTRCSKVEPPRERKHHSDSDTVEPLMKLERSKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A598</accession>
    <name>A0A673A598_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">gjb1a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A598"/>
    <dbReference type="Ensembl" id="ENSSORT00005024573.1">
        <property type="protein sequence ID" value="ENSSORP00005023873.1"/>
        <property type="gene ID" value="ENSSORG00005011532.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A598"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF20">
        <property type="entry name" value="GAP JUNCTION BETA-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="145"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="240" mass="27357" checksum="D5C45327941E744C" modified="2020-06-17" version="1">MNWASFYAVISGVNRHSTGIGRIWLSVLFIFRILVLVVAAESVWGDEKSGFTCNTQQPGCNSVCYDHFFPISHIRLWALQLILVSTPALLVAMHVAHRRHIDKRIYKLSGRTNPKDLEQIKSQKMKITGALWWTYVISLLFRIIFEVTFMYMFYMIYPGYKMIRLVKCDSYPCPNTVDCFVSRPTEKTVFTVFMLAASGVCILLNIAEVVFLVGKACSKHLNNAGDSSMGAWIQQKLCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5J1</accession>
    <name>A0A673A5J1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G-protein coupled receptors family 2 profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A5J1"/>
    <dbReference type="Ensembl" id="ENSSORT00005024642.1">
        <property type="protein sequence ID" value="ENSSORP00005023938.1"/>
        <property type="gene ID" value="ENSSORG00005011554.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A5J1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="1">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="54" mass="6345" checksum="2811525CAEDF4900" modified="2020-06-17" version="1">LVQRHLLSPDFHSVWLYCSRNWDGWMCWDDTPAGTLTTQNCPNYFADFDPTGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AB29</accession>
    <name>A0A673AB29_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AB29"/>
    <dbReference type="Ensembl" id="ENSSORT00005027568.1">
        <property type="protein sequence ID" value="ENSSORP00005026785.1"/>
        <property type="gene ID" value="ENSSORG00005012834.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AB29"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="80" mass="8722" checksum="A0A1480483BE3A89" modified="2020-06-17" version="1">MPGTLPTRLTTILSLLFPVRTLTALLMVMLSRLMPFTSTSLSPTRKPASSAKTHKKKPCSEPPRTLKPSFPSTLLSTVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AF62</accession>
    <name>A0A673AF62_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AF62"/>
    <dbReference type="Ensembl" id="ENSSORT00005029099.1">
        <property type="protein sequence ID" value="ENSSORP00005028290.1"/>
        <property type="gene ID" value="ENSSORG00005013539.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AF62"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134:SF5">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="467" mass="56148" checksum="4D0996B7AE3EEA5D" modified="2020-06-17" version="1">HRLALARIHTHTHTHTHRDRHTHIHTHTHLHTHTYTHTHTHTHTHTHTYTHTHTHTHTHTHTPTHTYTHTHTHTYTHTHTPTHTHTHTHTHTHIHTHTHTHTHTPTHTHTHTYTHTHTHTYTHTHIHTHTHTHIHTHTYTHTHTHLHTHTHTHTYTHTHTYTHTHTHIHTHTYTHTHTHTYTHTHTYTHTYTHTHTHIHTHTHTHIHTHTHTHTHTHTHTYTHTHTHIHTHTHTYTHTHTHTYTHTHIHTHTHTHTHTHTHTHTHTHTHTHTHIHTHTHIHTHIHTHIHTHIHTHTHTHIHTHTYTHTHTHTYTHTHTHTHTHIHTHTHTHTHTHTHTYTHTHTHTYTHTHTHTHTHIHTHTYTHTHIHTHTHTHTHTHIHTHTHIHTHTHTHTHTHTHTHTHIHTHTYTHTHTHTHTHIHTHTHTYTHTHTHTHTHTHTHTHTQKHASTGSSFFSSVSSYSRSALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AH26</accession>
    <name>A0A673AH26_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytohesin 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cyth3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AH26"/>
    <dbReference type="FunCoup" id="A0A673AH26">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029825.1">
        <property type="protein sequence ID" value="ENSSORP00005029005.1"/>
        <property type="gene ID" value="ENSSORG00005013490.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AH26"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01252">
        <property type="entry name" value="PH_GRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000003">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000009">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF320">
        <property type="entry name" value="CYTOHESIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="9">
        <location>
            <begin position="63"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="268"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="46849" checksum="E143D95F5C3BF0E6" modified="2020-06-17" version="1">MKAKGLLCFSSSCVSVPEDLSLEERDELSNIRRRKRELLDDIERLKFEIAEVMTEIEQLTCVGESKTSQRNKQIAMGRKKFNMDPKKGIQFLLENDLLQHTPEDIAQFLYKGEGLNKTVIGDYLGERDDFNIKVLQAFVELHEFADLNLVQALRSFLWSFRLPGEAQKIDRMMEAFASRYCQCNPGVFQSTDTCYVLSFAIIMLNTSLHNPNVRDKPPVERFISMNRGINEGGDLPEELLRNLYDSIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDEPRKPNCFELYNPNHKGQVIKACKTEADGRVVEGNHVVYRISAPTPEEKEEWIKSIKASISRDPFYDMLATRKRRIANKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJG3</accession>
    <name>A0A673AJG3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heat shock factor-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115428034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HSBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002670.1">
        <property type="nucleotide sequence ID" value="XM_030146810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AJG3"/>
    <dbReference type="FunCoup" id="A0A673AJG3">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029256.1">
        <property type="protein sequence ID" value="ENSSORP00005028447.1"/>
        <property type="gene ID" value="ENSSORG00005013614.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428034"/>
    <dbReference type="CTD" id="440498"/>
    <dbReference type="InParanoid" id="A0A673AJG3"/>
    <dbReference type="OrthoDB" id="4159489at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.430:FF:000003">
        <property type="entry name" value="Heat shock factor binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009643">
        <property type="entry name" value="HS1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424:SF0">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06825">
        <property type="entry name" value="HSBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="20"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="68" mass="7640" checksum="4B4D072586A4BD44" modified="2020-06-17" version="1">MSKTECKAAQDLTAAMEKTMQRLQGRFQAMSEQLESKVDEMGTRIDDLEKNVNELMTQAGMEQQGVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKD9</accession>
    <name>A0A673AKD9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">adck5</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002656.1">
        <property type="nucleotide sequence ID" value="XM_030146796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AKD9"/>
    <dbReference type="FunCoup" id="A0A673AKD9">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029943.1">
        <property type="protein sequence ID" value="ENSSORP00005029123.1"/>
        <property type="gene ID" value="ENSSORG00005013646.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428024"/>
    <dbReference type="CTD" id="203054"/>
    <dbReference type="InParanoid" id="A0A673AKD9"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF28">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="170"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="579" mass="66548" checksum="F7AA418BEDD281DC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMW5</accession>
    <name>A0A673AMW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TBC domain-containing protein kinase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tbck</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the FERRY complex (Five-subunit Endosomal Rab5 and RNA/ribosome intermediary). The FERRY complex directly interacts with mRNAs and RAB5A, and functions as a RAB5A effector involved in the localization and the distribution of specific mRNAs most likely by mediating their endosomal transport. The complex recruits mRNAs and ribosomes to early endosomes through direct mRNA-interaction. Also involved in the modulation of mTOR signaling and expression of mTOR complex components. Involved in the control of actin-cytoskeleton organization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AMW5"/>
    <dbReference type="FunCoup" id="A0A673AMW5">
        <property type="interactions" value="1218"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031887.1">
        <property type="protein sequence ID" value="ENSSORP00005031020.1"/>
        <property type="gene ID" value="ENSSORG00005014763.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AMW5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000015">
        <property type="entry name" value="TBC domain-containing protein kinase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000332">
        <property type="entry name" value="TBC domain-containing protein kinase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000018">
        <property type="entry name" value="TBC domain-containing protein kinase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000012">
        <property type="entry name" value="TBC domain-containing protein kinase-like protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF168">
        <property type="entry name" value="TBC DOMAIN-CONTAINING PROTEIN KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="12">
        <location>
            <begin position="467"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="13">
        <location>
            <begin position="786"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="889" mass="99967" checksum="5D165E8303F01E78" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANT8</accession>
    <name>A0A673ANT8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Si:ch211-145o7.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ANT8"/>
    <dbReference type="Ensembl" id="ENSSORT00005030925.1">
        <property type="protein sequence ID" value="ENSSORP00005030082.1"/>
        <property type="gene ID" value="ENSSORG00005014361.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ANT8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20031">
        <property type="entry name" value="FH_FOXN2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047119">
        <property type="entry name" value="FOXN2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13962:SF26">
        <property type="entry name" value="FORKHEAD BOX PROTEIN N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13962">
        <property type="entry name" value="FORKHEAD BOX PROTEIN N3-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="92"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="92"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="176" mass="19767" checksum="7DF8D6A4E2142C71" modified="2025-10-08" version="2">MSVKLPQSQRSQAPSLHSSSPLSHCTASQCLIGQNTQCLSPANTSDQDDLTCLSWLHQRGNLLPFHPLPKMTPLSQLESCTTTQPASPPSFKPPYSFSSLIFMAIEDSPDKRLPVKDIYEWIINNFPYYRTASGGWRNSVRHNLSLSKSFQRIQRDKNQVGTRDGLLNYSQIKSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANX4</accession>
    <name>A0A673ANX4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nudt22</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_030001962.1">
        <property type="nucleotide sequence ID" value="XM_030146102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ANX4"/>
    <dbReference type="FunCoup" id="A0A673ANX4">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030961.1">
        <property type="protein sequence ID" value="ENSSORP00005030117.1"/>
        <property type="gene ID" value="ENSSORG00005014382.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427517"/>
    <dbReference type="CTD" id="84304"/>
    <dbReference type="InParanoid" id="A0A673ANX4"/>
    <dbReference type="OrthoDB" id="242473at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052751">
        <property type="term" value="F:GDP-mannose hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02883">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000087">
        <property type="entry name" value="Nudix (nucleoside diphosphate linked moiety X)-type motif 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055295">
        <property type="entry name" value="NUDT22/NUDT9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31835">
        <property type="entry name" value="URIDINE DIPHOSPHATE GLUCOSE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31835:SF1">
        <property type="entry name" value="URIDINE DIPHOSPHATE GLUCOSE PYROPHOSPHATASE NUDT22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="206"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="390" mass="43207" checksum="A46FB222ECF7A12B" modified="2020-06-17" version="1">MMDPELSVLLHCADWRGLMESQIQVELSERFNRQTDPALERHIEELWTERISKEPWIFNGAKFRLHSFCLTSPKHPSCSESLHVPPLNCEEEEGGLNSRKTAEGLCTRDSLHPQNHLDQRNKFASQVENASCTPAPSSSTCGCKEEGVTASPSSKTTNNKDSENLLTLRLGLTSYKDYLGTNWSCQVEELRRRGEVEFGDPQALLAQPLGVGAVLCTDDGQVVLIRRSQRVAEAGGLLDIPGGHPEPKVVCERFGEVVSEEQINVEMLRPRSEAVVSELFSSICAEIRDEVNVPLTSLGEPVLMGIALNHTSAGRPSAEFYVSCSLTSDEVRKLYWKGGAEAHESTDIVFLSKNDMLQLDRSCPLWSELCPSAKGAMLLYQTVKPDGDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APZ6</accession>
    <name>A0A673APZ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">inorganic diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ppa2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029995904.1">
        <property type="nucleotide sequence ID" value="XM_030140044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673APZ6"/>
    <dbReference type="FunCoup" id="A0A673APZ6">
        <property type="interactions" value="1344"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031342.1">
        <property type="protein sequence ID" value="ENSSORP00005030487.1"/>
        <property type="gene ID" value="ENSSORG00005014536.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423286"/>
    <dbReference type="CTD" id="27068"/>
    <dbReference type="InParanoid" id="A0A673APZ6"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="343" mass="38921" checksum="8490A87BCFA80FD1" modified="2020-06-17" version="1">MRPLLQCCAFGCSVTHFRALPAFRNKSVTQAAAAFQLHLRKTMHYQTEERGHPNSPDYRIYFKNSGGKYISPFHDIPLIAETEQDNDVPAKKPKKPESELLFNMVVEVPRWSNAKMEIATKEPLNPIKQDVKKGKLRYVANIFPHKGYIWNYGALPQTWEDPNHTDKDTKCCGDNDPIDVCDIGTQVCYPGQVIQVKVLGILAMIDEGEMDWKVIAINAEDPDAKNLNGIEDVRKSRPGHLEATVDWFRKYKVPDGKPENQFGFKGQFKDKDFAVEIIRSTHEHWRALVQRQTDGGDIECQNISCCESPFKCSSDKARDVVQSAPVLGSAYPVSPDVDKWHFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQW4</accession>
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        <submittedName>
            <fullName evidence="3">Transmembrane protein 64</fullName>
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        <name evidence="3" type="primary">LOC115428693</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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            <dbReference type="Pfam" id="PF09335"/>
        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="358" mass="38940" checksum="218656EE4846A703" modified="2020-06-17" version="1">MAVSGSTTVQLFTKLLKHAVGKTQIQLNRWLQRTATEECDKIDILICSAFDERRNSLGKSDGDPEVITDSGVTTFTTTGEFRHPCCITTCCFKSALLACILTAVCFSSVALVRQYLKDLLLWVESMDSLVGAMLFIVGLIIVSFPCGWGYIVLNVAAGYLYGFVLGMGLVMVGVLIGTFVAHLVCKRLLTDWVLNKVGNSEQLSAVIRVVEGGSGLKVVALARLTPIPFGLQNAVFSITDVSLPNYLVASSVGLLPTQLLNSYLGTTLRTMEDVIAEQSVSGYFVFSLQIIISIGLMFYVVHRAQVELNAAITACQMELKSSHMNGSSTNHSSFSYCSKRVTTSSGKLHQQQWCSEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQX9</accession>
    <name>A0A673AQX9_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Transmembrane protein 203-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">tmem203</name>
        <name evidence="3" type="synonym">LOC115416308</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
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            <taxon>Actinopterygii</taxon>
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    </organism>
    <reference evidence="2" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="FAM11A, B PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="34"/>
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            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="138" mass="16034" checksum="35910E37D2D1DA55" modified="2020-06-17" version="1">MLFSLRELVQWLGFATFELFLHLLALLVFSMLVALRADMFTPSLNWWLVFVPLFAADGLSTYFTAIVSIRLYQENEKRLAVLRLLWVLTVLSLKLVCEVLLCQKLAEQEQARDLWFGLIVSPLFILLQLLMIRACRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARR9</accession>
    <name>A0A673ARR9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G protein-coupled receptor 119</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gpr119</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A673ARR9"/>
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        <property type="protein sequence ID" value="ENSSORP00005032009.1"/>
        <property type="gene ID" value="ENSSORG00005015202.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <begin position="179"/>
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            <end position="280"/>
        </location>
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            <end position="309"/>
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            <begin position="64"/>
            <end position="83"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023170"/>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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            <dbReference type="RuleBase" id="RU000688"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="387" mass="43342" checksum="5CEE9E9E77A44593" modified="2020-06-17" version="1">MGMILTIASCLIISTNIFVAVALLKLLLRKSSQSWCFVLNLAVADTLVGVAITGLATEDFNGSGNNVTQSRQSNIPADQLTSSSSSIQGRTRCLIRMAFVTSPCTASIMSMFLISLDRYAAIKMPLRYSQRCGKGTAVGCLVALWICAITLGFLPVMVRSLQYEHYNGVCAFFSVIDDVGIIILYSVCFFPVFSVFVYIYLDILKIACSHQKQICQVRQAGSRTAEHQSHPHHHHHHHQQQQLKSRYWSHVKALRTVAVLVGCFLILWCPFFVVCIVALLCETCELTELLENHLWLLGLSNSLINPLVYAFWQKEVRMQIAAMFSCFTGRPSATAASGVTGRCEPQPSIDNHTAVSAKYRKQSLHSKNTLTFLERRRREEEKLKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASX0</accession>
    <name>A0A673ASX0_9TELE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ASX0"/>
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        <property type="protein sequence ID" value="ENSSORP00005032770.1"/>
        <property type="gene ID" value="ENSSORG00005015553.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASX0"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="entry name" value="EPSTI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22529:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22529">
        <property type="entry name" value="EPITHELIAL-STROMAL INTERACTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="125" mass="14461" checksum="43DC0BA46C897D08" modified="2020-06-17" version="1">MKCNGAYCKRCIKMKMLKKTRKDMTRKRSKSKIVWFATALYDHHKAFTVIPPNPKKRQEIQRKAEAELAALEELRLSRAMAYVSINPSSVGKEMTNQTKHHDTDRKCVNNIDSLIPMVPLTDLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVI0</accession>
    <name>A0A673AVI0_9TELE</name>
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        <submittedName>
            <fullName evidence="22">Solute carrier family 8 member 4a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">slc8a4a</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AVI0"/>
    <dbReference type="FunCoup" id="A0A673AVI0">
        <property type="interactions" value="27"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005033670.1"/>
        <property type="gene ID" value="ENSSORG00005015941.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:sarcolemma"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000001">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032452">
        <property type="entry name" value="Na_Ca_Ex_C-exten"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00845">
        <property type="entry name" value="caca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF73">
        <property type="entry name" value="NCX4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16494">
        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="721"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="823"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="855"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="894"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="21">
        <location>
            <begin position="380"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="21">
        <location>
            <begin position="522"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="922" mass="101497" checksum="98BB69B747D4B97B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWW8</accession>
    <name>A0A673AWW8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Myeloid ecotropic viral integration site 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">meis3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/MEIS homeobox family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A673AWW8"/>
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        <property type="protein sequence ID" value="ENSSORP00005033754.1"/>
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    </evidence>
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    </evidence>
    <sequence length="436" mass="47489" checksum="3EED5BD2689CBC42" modified="2020-06-17" version="1">MSDSTDVKCVSCFLSFHLIVFQYEELVHYSGSEGMSVGGYGDDVRPLPPPHYGATIPDSLKHHKDQIYGHPLFPLLALVFEKCELATCSPRDSTSLSATSHLPGMTNHSDVCSSESFNDDIAAFAKQIRSEKPIFSSNPELDNLMIQAIQVLRFHLLELEKVHDLCDNFCHRYITCLKGKMPTDLVLDDREGGSKSDMEDFTGSCTSLSEQNASWLRDPDECATTPLGTPGTCGLPSHSTADNCSDTGDGLDGGVASPSTGEEDETDRERRNNKKRGIFPKVATNIMRAWLFQHLSHPYPSEEQKKQLSQDTGLTILQVNNWFINARRRIVQPMIDQSNRSGQGGPYSPEGAALGGYGLDGQAHLGLRTAGLQGMSSLQGDYPGALLSQPGYPPHPGPSLHAYPGPHPHAAMLLHPPPHAHPAEPLLAQGLDIHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYE4</accession>
    <name>A0A673AYE4_9TELE</name>
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        <recommendedName>
            <fullName evidence="11">Ras-responsive element-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rreb1a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005035750.1">
        <property type="protein sequence ID" value="ENSSORP00005034826.1"/>
        <property type="gene ID" value="ENSSORG00005016414.1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Ras responsive element binding protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras responsive element binding protein 1a"/>
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        <property type="entry name" value="Ras-responsive element binding protein, putative"/>
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    </dbReference>
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        <property type="entry name" value="ras-responsive element-binding protein 1"/>
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        <property type="entry name" value="ras-responsive element-binding protein 1 isoform X1"/>
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        <property type="entry name" value="ras-responsive element-binding protein 1 isoform X1"/>
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        <property type="entry name" value="ras-responsive element-binding protein 1 isoform X1"/>
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        <property type="entry name" value="ras-responsive element-binding protein 1 isoform X2"/>
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        <property type="entry name" value="Ras-responsive element-binding protein 1a"/>
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        <property type="entry name" value="Ras-responsive element-binding protein 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052795">
        <property type="entry name" value="RREB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46451">
        <property type="entry name" value="RAS-RESPONSIVE ELEMENT-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46451:SF1">
        <property type="entry name" value="RAS-RESPONSIVE ELEMENT-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="226"/>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="420"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="764"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="792"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="820"/>
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        </location>
    </feature>
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        <location>
            <begin position="890"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="920"/>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1294"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1557"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1676"/>
            <end position="1703"/>
        </location>
    </feature>
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        <location>
            <begin position="1704"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="1726"/>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1635"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1748"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1776"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1823"/>
            <end position="1867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1898" mass="201115" checksum="B7AC30BDD34B6A65" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZ73</accession>
    <name>A0A673AZ73_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ABC-type glutathione-S-conjugate transporter</fullName>
            <ecNumber evidence="14">7.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115416017</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.3" evidence="14"/>
    <dbReference type="Ensembl" id="ENSSORT00005036091.1">
        <property type="protein sequence ID" value="ENSSORP00005035156.1"/>
        <property type="gene ID" value="ENSSORG00005016398.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZ73"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000293">
        <property type="entry name" value="ATP binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005292">
        <property type="entry name" value="MRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00957">
        <property type="entry name" value="MRP_assoc_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF339">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="498"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="905"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="947"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1032"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1129"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="282"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="598"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="907"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="1224"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1462" mass="163710" checksum="CA15CE6BAA09505F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B126</accession>
    <name>A0A673B126_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B126"/>
    <dbReference type="Ensembl" id="ENSSORT00005036466.1">
        <property type="protein sequence ID" value="ENSSORP00005035516.1"/>
        <property type="gene ID" value="ENSSORG00005016763.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036485.1">
        <property type="protein sequence ID" value="ENSSORP00005035535.1"/>
        <property type="gene ID" value="ENSSORG00005016778.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF112">
        <property type="entry name" value="SI:CH211-113E8.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="16"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="268" mass="29803" checksum="5352579B648A5B65" modified="2020-06-17" version="1">MSQGIRPVGGCVFFAGPPVRIVMIGKTGVGKSAVGNTIVNKKAFISLESPESVTAHCRHAQLEGEKREVHVVDTPGILDTSKSVEVVKKEIIKCIQMSTPGPHVFLLVLQIGRFTKEEENCVDALEKLFGPEMSKYMIVLFSHGDKLTQQGTTIFDYLRSGHPKLTELLNRCGNRFHVFNNKDKKNRKQVAELIKKIDEMVAANRASYYSDDMFEAVDRTQRQPDKLPDGTEDLNVNVLSELLQRIIQFQATLSATTPAPAAVYDNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B349</accession>
    <name>A0A673B349_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115423245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAMHD1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B349"/>
    <dbReference type="Ensembl" id="ENSSORT00005036729.1">
        <property type="protein sequence ID" value="ENSSORP00005035774.1"/>
        <property type="gene ID" value="ENSSORG00005016886.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B349"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="132"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="551" mass="64324" checksum="19AE74C86684F897" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4X0</accession>
    <name>A0A673B4X0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor AP-2-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">AP-2 transcription factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Activating enhancer-binding protein 2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Activator protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">tfap2a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979220.1">
        <property type="nucleotide sequence ID" value="XM_030123360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B4X0"/>
    <dbReference type="Ensembl" id="ENSSORT00005037350.1">
        <property type="protein sequence ID" value="ENSSORP00005036389.1"/>
        <property type="gene ID" value="ENSSORG00005017148.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411285"/>
    <dbReference type="CTD" id="7020"/>
    <dbReference type="InParanoid" id="A0A673B4X0"/>
    <dbReference type="OrthoDB" id="6252992at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008121">
        <property type="entry name" value="TF_AP2_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01749">
        <property type="entry name" value="AP2ATNSCPFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="16">
        <location>
            <begin position="226"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="452" mass="49268" checksum="2027EC464D0FE2A3" modified="2020-06-17" version="1">MEDSSASESEVENKRTSGRNVPVEMPVASSAGCSPSSEDRHDGTSNGTARLPQLGGVGQSPYTSAPPLSHTPNSDFQPPYFPPPYQPIYPQSQDPYSHVNDPYSLNSLHTQPQPQHPGWPGQRQQESGLLHQHRSLPHQLCREYRREVLLPSGHGIDTGLSDSIPIHGIPHSLEDVQPVEDQGIHIPDQTVIKKGPVSLSKNNNVSAIPVNKDGLFGGVVNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAVAEYVNRQHSDPNEQVQRKNMLLATKQVCKEFTDLLSQDRSPLGNSRPQPILEPGIQSCLTHFSLISHGFGTPALCAAVTALQNYLTEAIKAMDKMYLNNNPNSHSDNGTKGGDKDEKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B712</accession>
    <name>A0A673B712_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box only protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fbxo9</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and acts as a regulator of mTOR signaling.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO9).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030012308.1">
        <property type="nucleotide sequence ID" value="XM_030156448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B712"/>
    <dbReference type="FunCoup" id="A0A673B712">
        <property type="interactions" value="567"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037214.1">
        <property type="protein sequence ID" value="ENSSORP00005036253.1"/>
        <property type="gene ID" value="ENSSORG00005017107.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434480"/>
    <dbReference type="CTD" id="26268"/>
    <dbReference type="InParanoid" id="A0A673B712"/>
    <dbReference type="OrthoDB" id="2117972at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22089">
        <property type="entry name" value="F-box_FBXO9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000012">
        <property type="entry name" value="F-box only protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045464">
        <property type="entry name" value="Hrt3/FBXO9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF29">
        <property type="entry name" value="F-BOX ONLY PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19270">
        <property type="entry name" value="FBO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="159"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="422" mass="49090" checksum="2C33B20F92F1ECE2" modified="2020-06-17" version="1">MAEENADIGGAVEDEDEGSDDPNLQLELNAFRARWMSELKPSSGASGGRLTHVKGLKRTLEVAREEKATELFLRAVHEEQKGAMYEAIKYYRMSMQLVPDIEFKVNYSRTPDGERVGGHYMQESGVDGEIEDLLAYFEQQLTLECSFPKICTPEQEMTRVHISALPREILMYIFRWVVSSDLDMRALEQLSLVCRGFYICARDPEIWRLACLRVWGRNCTKLGPFKSWREMFLQRPRVRFDGVYISKTSYIRQGEQSLDGFYRAWHHVEYYRYLRFFPDGQVMMLTTPEDPLSVVPRLRTANSRIDSVLLGHFRLSQETDNQTKVYAVVCKKKEERATEITRNRFYRRNPAPEAEHSFHVGLHLSSGGRQSFSKLVWIHHSCHITYKLTGETVVTSFDLDRMYSPFFFARVKSYTAFSEQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B8A5</accession>
    <name>A0A673B8A5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nfkbie</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029985100.1">
        <property type="nucleotide sequence ID" value="XM_030129240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B8A5"/>
    <dbReference type="Ensembl" id="ENSSORT00005039554.1">
        <property type="protein sequence ID" value="ENSSORP00005038561.1"/>
        <property type="gene ID" value="ENSSORG00005018014.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415610"/>
    <dbReference type="CTD" id="4794"/>
    <dbReference type="InParanoid" id="A0A673B8A5"/>
    <dbReference type="OrthoDB" id="10254947at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
        <property type="term" value="P:cellular response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051070">
        <property type="entry name" value="NF-kappa-B_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680:SF5">
        <property type="entry name" value="NFKB INHIBITOR EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="283"/>
            <end position="315"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="351" mass="38509" checksum="DA12E2028F1C9AF1" modified="2020-06-17" version="1">MSRDGCGKDDLLEDNRTDSGIDSYRSILKSEEPREPSTDFSGPRDKLSATGEERLDSAYASSSLTVESLSDIVGVCSLSSPREEQEEEEDSREEECGQDDNPLTIITEDGDTILHLAIIHEEERIAQRLIEIFPKEVLDIQNNLYQSPLHLATYLNLTEVVKGLVEKGASLQLQDHDGNTALHVACQHGQTQCVSHMTQNVSPSKLAPVLETQNWRGLTCLHLAALNRQHQIMKLLMKMDAELNIQEGTSGKTALHLAVEMHDITSVKLLLSKGASVDAAMFNGCTPLHLAVGRQDATIANLLCQSGADMMLRNMEDETALDLADGNDDILALFPFDDIQISGRSVVGVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BA92</accession>
    <name>A0A673BA92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 51</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lrrc51</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BA92"/>
    <dbReference type="Ensembl" id="ENSSORT00005039205.1">
        <property type="protein sequence ID" value="ENSSORP00005038214.1"/>
        <property type="gene ID" value="ENSSORG00005017880.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BA92"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="180" mass="20297" checksum="5C6777A2464EFF69" modified="2020-06-17" version="1">MDGAPVDLSFKCINRLADALAEEPSCGLRPVKRNAEGKYLSRSLRLSNNSISGLNDLQMTLSHFLAQPSQLAWLDLSFNKISHIDQVLCELHELRVLYLHGNCIFILSEVDRLGALPHLHTLTLHGNDIETNKGYRNHVVSALPHLKMMDFSGVTRQERVLANVWHHSSNRSRSSKDTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAG3</accession>
    <name>A0A673BAG3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BAG3"/>
    <dbReference type="Ensembl" id="ENSSORT00005038587.1">
        <property type="protein sequence ID" value="ENSSORP00005037607.1"/>
        <property type="gene ID" value="ENSSORG00005017648.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BAG3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF27">
        <property type="entry name" value="SCAVENGER RECEPTOR CYSTEINE-RICH TYPE 1 PROTEIN M130-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="96"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="258"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="362"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="470"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="570"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="686"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="10"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="283"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="387"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="400"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="711"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="724"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="809" mass="87741" checksum="706DC09E461163F8" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB37</accession>
    <name>A0A673BB37_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BB37"/>
    <dbReference type="FunCoup" id="A0A673BB37">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039486.1">
        <property type="protein sequence ID" value="ENSSORP00005038494.1"/>
        <property type="gene ID" value="ENSSORG00005017984.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BB37"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF470">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="65"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="342" mass="39196" checksum="D9A12CF36AF93904" modified="2020-06-17" version="1">MDNICNRRGLITHSNSSTMFSPRTLLNTSVIIHPPGFYIIGFETFPYISIYLIFLAFVYAVTVLFNIFLICIIIYDHSLHTPKFLAVINLAVVDLLLNTCTIPSMIKVFLIKDNFVPFNLCLVQMYFYYAILAMESYSLAILAYDRFVAICFPLHQHSINTLWSMSCIVGFTWIFNLGRLGYSVSIMTRLSFCNSLRVMSYFCDYAPVFRLACNDYSLQWSVASVSSMVNLIAPFSFIFLSYVCILVTVLRMKSVSNRIKVFTTCIEHLIVVAIFYLPIFSIFFIGLYLRVIDPDQRVLSLSLASCITPCINPIVYSLKTKEIKARAVTLQSDHNMTNFYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCI7</accession>
    <name>A0A673BCI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 7B, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytochrome c oxidase polypeptide VIIb</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115416326</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase VIIb family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029985931.1">
        <property type="nucleotide sequence ID" value="XM_030130071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BCI7"/>
    <dbReference type="Ensembl" id="ENSSORT00005041228.1">
        <property type="protein sequence ID" value="ENSSORP00005040191.1"/>
        <property type="gene ID" value="ENSSORG00005018746.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416326"/>
    <dbReference type="CTD" id="1349"/>
    <dbReference type="InParanoid" id="A0A673BCI7"/>
    <dbReference type="OrthoDB" id="9937520at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01403">
        <property type="entry name" value="Cyt_c_Oxidase_VIIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.51.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 7B, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.51.10">
        <property type="entry name" value="Cytochrome C Oxidase, chain K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008433">
        <property type="entry name" value="Cyt_c_oxidase_suVIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023272">
        <property type="entry name" value="Cyt_c_oxidase_suVIIB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16716">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16716:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05392">
        <property type="entry name" value="COX7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81423">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="80" mass="8928" checksum="519AAB5B443D305C" modified="2020-06-17" version="1">MFRFAKNAVNLTGQAVRQVRQVRHGSSAPDFHDKYGNGLMVGGAVFCVAVWTFVVTQTGITWNTSPIGKVMPKEWRESEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BGI2</accession>
    <name>A0A673BGI2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BGI2"/>
    <dbReference type="Ensembl" id="ENSSORT00005041429.1">
        <property type="protein sequence ID" value="ENSSORP00005040389.1"/>
        <property type="gene ID" value="ENSSORG00005018860.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BGI2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006970">
        <property type="entry name" value="PT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04886">
        <property type="entry name" value="PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="134" mass="15328" checksum="64B7116CF260FE7C" modified="2020-06-17" version="1">MDGWMTMYVMQLRCCPLLLGDRAGWVSKAELVELISLVESIKLVVQPTVWPTVRPTVQSTVRPTVWPTVRPTVRPTVQPTVRPTVWPTVQPTVRPTVRPTVQPTVRIYLENKAQFCYIFFVSLLSFYTISNKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BL59</accession>
    <name>A0A673BL59_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="12">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pin4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="12"/>
    <dbReference type="RefSeq" id="XP_030002200.1">
        <property type="nucleotide sequence ID" value="XM_030146340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BL59"/>
    <dbReference type="FunCoup" id="A0A673BL59">
        <property type="interactions" value="962"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042384.1">
        <property type="protein sequence ID" value="ENSSORP00005041322.1"/>
        <property type="gene ID" value="ENSSORG00005019230.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427684"/>
    <dbReference type="CTD" id="5303"/>
    <dbReference type="InParanoid" id="A0A673BL59"/>
    <dbReference type="OrthoDB" id="1911748at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000015">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043323">
        <property type="entry name" value="PIN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45995">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13616">
        <property type="entry name" value="Rotamase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 12" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PpiC" evidence="14">
        <location>
            <begin position="31"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="127" mass="13544" checksum="DCADFD237E00C9BE" modified="2020-06-17" version="1">MPPKGKGGKGGKGAASGSAEAEKKDKTPKGGTAVKVRHILCEKHGKCMEAMEKLKAGVRFSEVASQYSEDKARQGGDLGWMTRGSMVGPFQDAAFALPVSTMDKPVYTDPPVKTKFGYHIIMVEGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BP21</accession>
    <name>A0A673BP21_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein Aiolos-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115427996</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Ikaros C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002627.1">
        <property type="nucleotide sequence ID" value="XM_030146767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BP21"/>
    <dbReference type="Ensembl" id="ENSSORT00005043425.1">
        <property type="protein sequence ID" value="ENSSORP00005042347.1"/>
        <property type="gene ID" value="ENSSORG00005019631.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427996"/>
    <dbReference type="InParanoid" id="A0A673BP21"/>
    <dbReference type="OrthoDB" id="6417347at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000073">
        <property type="entry name" value="IKAROS family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000080">
        <property type="entry name" value="IKAROS family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000372">
        <property type="entry name" value="IKAROS family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN AIOLOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="86"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="529" mass="58801" checksum="E90C648901566AFE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BPW6</accession>
    <name>A0A673BPW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">pancreatic elastase</fullName>
            <ecNumber evidence="11">3.4.21.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115419679</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.36" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A673BPW6"/>
    <dbReference type="FunCoup" id="A0A673BPW6">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044358.1">
        <property type="protein sequence ID" value="ENSSORP00005043264.1"/>
        <property type="gene ID" value="ENSSORG00005019851.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BPW6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000122">
        <property type="entry name" value="Chymotrypsin-like elastase family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050850">
        <property type="entry name" value="Peptidase_S1_Elastase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257:SF0">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="43"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="32528" checksum="3E3CFE4939D9B168" modified="2020-06-17" version="1">MLELIIDICQAAKIKYCISSLLSVLAELEPQPRYLEDTVEERVVGGEVARPNSWPWQISLQYKSGSSFYHTCGGTLIRRGWVMTAAHCVDRSRTWRVVLGDHNINSHEGREQYMSVSRVYLHPNWNSNNVAGGWDIALLRLSSDATLNNYVQLGALPPSGQVLPHNNPCYISGWGRTQTGGQLSAQLKQAYLPVVDHKTCSSYGWWGSTVKNSMVCAGGGSESGCQGDSGGPLNCSVGGKWVVHGVTSFVSSSGCNAYRKPTVFTRVSAYIIFTSWRLSPLIGFINRIAVNIICFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQS1</accession>
    <name>A0A673BQS1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Supervillin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">svild</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005044653.1">
        <property type="protein sequence ID" value="ENSSORP00005043554.1"/>
        <property type="gene ID" value="ENSSORG00005019692.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQS1"/>
    <dbReference type="OrthoDB" id="28894at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="term" value="P:actin polymerization or depolymerization"/>
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        <property type="term" value="P:barbed-end actin filament capping"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
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        <property type="entry name" value="Severin"/>
        <property type="match status" value="5"/>
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    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
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    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
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    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR11977:SF87">
        <property type="entry name" value="SUPERVILLIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GEL"/>
        <property type="match status" value="4"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HP" evidence="4">
        <location>
            <begin position="1412"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="601"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="99"/>
            <end position="115"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
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        <location>
            <begin position="162"/>
            <end position="172"/>
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        <location>
            <begin position="180"/>
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        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
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        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="300"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1475" mass="165518" checksum="C517BCAEEC575F25" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSC5</accession>
    <name>A0A673BSC5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">ATP-binding cassette sub-family G member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Sterolin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">abcg5</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>cholesterol(in) + ATP + H2O = cholesterol(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39051"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:39052"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>sitosterol(in) + ATP + H2O = sitosterol(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27693"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:39104"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Heterodimer with ABCG8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
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        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045204.1">
        <property type="protein sequence ID" value="ENSSORP00005044084.1"/>
        <property type="gene ID" value="ENSSORG00005020319.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BSC5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03234">
        <property type="entry name" value="ABCG_White"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001069">
        <property type="entry name" value="ATP-binding cassette, sub-family G (WHITE), member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF113">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY G MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="475"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="632"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="23">
        <location>
            <begin position="49"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00065141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="659" mass="73976" checksum="C7584EE70B0B5F43" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTB2</accession>
    <name>A0A673BTB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein jagged-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Protein jagged-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">jag1b</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BTB2"/>
    <dbReference type="FunCoup" id="A0A673BTB2">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047232.1">
        <property type="protein sequence ID" value="ENSSORP00005046076.1"/>
        <property type="gene ID" value="ENSSORG00005021049.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BTB2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042491">
        <property type="term" value="P:inner ear auditory receptor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090596">
        <property type="term" value="P:sensory organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000007">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000061">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000117">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000148">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000181">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000229">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000431">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3510:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000146">
        <property type="entry name" value="Putative neurogenic locus notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056986">
        <property type="entry name" value="JAG1_1/2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026219">
        <property type="entry name" value="Jagged/Serrate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF12">
        <property type="entry name" value="DELTA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23575">
        <property type="entry name" value="JAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02059">
        <property type="entry name" value="JAGGEDFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025508475" description="Protein jagged-1" evidence="20">
        <location>
            <begin position="24"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1037"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="211"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="265"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="306"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="344"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="382"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="420"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="457"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="495"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="561"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="599"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="637"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="675"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="714"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="752"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="790"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1121"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1158"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="625"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="663"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="778"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="816"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1193" mass="130394" checksum="6517E0D99CE39A75" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTB5</accession>
    <name>A0A673BTB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Potassium inwardly rectifying channel subfamily J member 1a, tandem duplicate 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">kcnj1a.1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BTB5"/>
    <dbReference type="FunCoup" id="A0A673BTB5">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046156.1">
        <property type="protein sequence ID" value="ENSSORP00005045020.1"/>
        <property type="gene ID" value="ENSSORG00005020686.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BTB5"/>
    <dbReference type="OrthoDB" id="273257at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000002">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003268">
        <property type="entry name" value="K_chnl_inward-rec_Kir1.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF6">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01321">
        <property type="entry name" value="KIR11CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 15" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="18">
        <location>
            <begin position="24"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="19">
        <location>
            <begin position="173"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="14">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="42267" checksum="310C66EE6F5E575D" modified="2020-06-17" version="1">MFGSLSKRIQNYLAERRSRRTRLVTKDGRCNIEYGNIKYSRRFAFLADFWTTFVEIRWRFVLVFFIGSFTLSWFIFGLLWYWIARSNGDLTWQNPPDGHTACVDNVIGLTTAFLYSLETQTTIGYGGRAITPLCPGAVALIIIQSLVGAIINCFMCGVILAKISLPKKRAKTIKFSEMAVISPKNGTLCLSIRLANLRKTLMIGSQIYGKLLRTTITPDGETIIMDQVNIDFMVDAGKDNLFFVCPLTLYHVINKSSPFFEMAVDTLHKQEFELVVFLDGTAESTSSSCQVRTSFIPQEIMWGYNFLPIISRSKEGKYRVDFSNFSKVVPVATAHCAYCFHNIKGHHHHSRDGHDNQGFEVIDINDPPNATKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTW0</accession>
    <name>A0A673BTW0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dnajc14</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030133886.1"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047455.1">
        <property type="protein sequence ID" value="ENSSORP00005046296.1"/>
        <property type="gene ID" value="ENSSORG00005021208.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A673BTW0"/>
    <dbReference type="OrthoDB" id="1507364at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050780">
        <property type="term" value="F:dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
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        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Jiv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052317">
        <property type="entry name" value="Viral_replicn-host_int_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44665">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44665:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
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    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="377"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="664"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="664" mass="74475" checksum="7196118897F7C45B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUC8</accession>
    <name>A0A673BUC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">N-acetyltaurine hydrolase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Phosphotriesterase-related protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pter</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-acetyl-L-isoleucine + H2O = L-isoleucine + acetate</text>
            <dbReference type="Rhea" id="RHEA:81119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:133735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:81120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-acetyl-L-leucine + H2O = L-leucine + acetate</text>
            <dbReference type="Rhea" id="RHEA:81115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:58270"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:81116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-acetyl-L-methionine + H2O = L-methionine + acetate</text>
            <dbReference type="Rhea" id="RHEA:67440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:71670"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67441"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-acetyl-L-valine + H2O = L-valine + acetate</text>
            <dbReference type="Rhea" id="RHEA:81123"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:133716"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:81124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-acetyltaurine + H2O = taurine + acetate</text>
            <dbReference type="Rhea" id="RHEA:81107"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:133737"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:81108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-propanoyltaurine + H2O = propanoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:81111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:231795"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:81112"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016999.1">
        <property type="nucleotide sequence ID" value="XM_030161139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BUC8"/>
    <dbReference type="FunCoup" id="A0A673BUC8">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045859.1">
        <property type="protein sequence ID" value="ENSSORP00005044729.1"/>
        <property type="gene ID" value="ENSSORG00005020579.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437794"/>
    <dbReference type="CTD" id="9317"/>
    <dbReference type="InParanoid" id="A0A673BUC8"/>
    <dbReference type="OrthoDB" id="9998343at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00530">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001559">
        <property type="entry name" value="Phosphotriesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819">
        <property type="entry name" value="PHOSPHOTRIESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819:SF3">
        <property type="entry name" value="PHOSPHOTRIESTERASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02126">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51347">
        <property type="entry name" value="PHOSPHOTRIESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601559-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="349" mass="38895" checksum="C9BD8D7A2B24AD26" modified="2020-06-17" version="1">MSKLSGKVQTVLGLVDPDQLGRTMTHEHLTMSFECCYFPPSPGDEAVAENPFQMQHMYWLRQNQYSCHENLLLHQEVGAVREELLAYRKAGGGAIVENTTTGIERDMPTLKQLAKDTGVHIIAGAGYYVDCTHSETTRKTSVEKLTDIIISEVLHGADGTDIRCGVIGEIGTGWPITDSEKKVLKATAHAQAQLGCPVIIHPGRDPAAPAEVVRILQEAGGDIRKTVMSHLDRTIFDNGELLEFAKLGSYLEYDLFGVEMLNYSYNLSVDMPSDSQRVQALAFLVKEGYEDRIVIAHDIHTKNRLTKFGGHGYSHILKNIVPKMLRRGISQHQVDKILIDNPKEWLTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVJ5</accession>
    <name>A0A673BVJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nfil3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030005956.1">
        <property type="nucleotide sequence ID" value="XM_030150096.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030005957.1">
        <property type="nucleotide sequence ID" value="XM_030150097.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030005958.1">
        <property type="nucleotide sequence ID" value="XM_030150098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BVJ5"/>
    <dbReference type="FunCoup" id="A0A673BVJ5">
        <property type="interactions" value="883"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048082.1">
        <property type="protein sequence ID" value="ENSSORP00005046911.1"/>
        <property type="gene ID" value="ENSSORG00005021476.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430187"/>
    <dbReference type="CTD" id="4783"/>
    <dbReference type="InParanoid" id="A0A673BVJ5"/>
    <dbReference type="OrthoDB" id="6151507at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14694">
        <property type="entry name" value="bZIP_NFIL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047229">
        <property type="entry name" value="NFIL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047106">
        <property type="entry name" value="NFIL3-like_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010533">
        <property type="entry name" value="Vert_IL3-reg_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284">
        <property type="entry name" value="NUCLEAR FACTOR INTERLEUKIN-3-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284:SF1">
        <property type="entry name" value="NUCLEAR FACTOR INTERLEUKIN-3-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06529">
        <property type="entry name" value="Vert_IL3-reg_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="69"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="245"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="445" mass="49190" checksum="D605B6633BBE5489" modified="2020-06-17" version="1">MTANRDNMQSIKQEVDSSESYSGEDALVLAVALQGSNRDLMDHNLSTMPFKAKSSCRRKREFIPEEKKDNLYWERRRKNNEAAKRSREKRRINDMVLENKLMALGEENASLKAELLSLKLKFGLLSSAAYAQEVQKLSSSANLYQEFVPPSSSSSQSSSSRDLEPLHMRSSCISVIKHSPHSTLPDLPETCTTTQGSFNICRAAEVKQEPAESSSYAQERSSPYEMYRNYVSGVYPQPASFLQITRSSSNSPRSSDDGVISKSSDGEDEQQVPKGPVPSVADSRSVIVSTHKVPDPNASALPHKLRIKARTIQIKVEAVDPEYESSGKSTSPVNEGYQSAPDSSEYSQSSMCPLSVQVNNMHNWSHRSEQWHKETLTPSPALNGTEPSYAHSDTEDSYLKQGLGGMSANVASLNRLITTRQESVIESVKSTADQYESLSKGCPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BY06</accession>
    <name>A0A673BY06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein</fullName>
            <shortName evidence="9">Cnt-b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Centaurin-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115423220</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">GTPase-activating protein for the ADP ribosylation factor family.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endosome membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate protein binding to PIP2 or PIP3 containing membranes.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BY06"/>
    <dbReference type="Ensembl" id="ENSSORT00005046683.1">
        <property type="protein sequence ID" value="ENSSORP00005045532.1"/>
        <property type="gene ID" value="ENSSORG00005019075.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BY06"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08850">
        <property type="entry name" value="ArfGap_ACAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13250">
        <property type="entry name" value="PH_ACAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000007">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000025">
        <property type="entry name" value="arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000020">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000026">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF407">
        <property type="entry name" value="ARF-GAP WITH COILED-COIL, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="1 9" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="257"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="12">
        <location>
            <begin position="392"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="688"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="721"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="528"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="625"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="847" mass="95233" checksum="ED83F50FDA43F29B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYX9</accession>
    <name>A0A673BYX9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glucagon receptor b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115410317</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BYX9"/>
    <dbReference type="Ensembl" id="ENSSORT00005047047.1">
        <property type="protein sequence ID" value="ENSSORP00005045892.1"/>
        <property type="gene ID" value="ENSSORG00005021053.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BYX9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004967">
        <property type="term" value="F:glucagon receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000133">
        <property type="entry name" value="Glucagon receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003290">
        <property type="entry name" value="GPCR_2_GLP1/glucagon_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF30">
        <property type="entry name" value="GLUCAGON RECEPTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01353">
        <property type="entry name" value="GLUCAGNFAMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="14">
        <location>
            <begin position="20"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="117"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="52921" checksum="17ADDF477357C738" modified="2020-06-17" version="1">MANGTVLEETYRKWVQYKDDCMKMIDTEPFSQAGLFCNRTFDRYACWPDTPAGFIINISCPFYLPWYSKVAQGVVRRWCGTNGIWKTDDSGEVWRDMTQCEDEKEATLWFKQLMVRFRILYTVGYSLSLFTLITALIILLSFRKLHCTRNYIHANLFLSFILRAVSVMVKDTMLERHWGREIMKHSDVSEMLSHQAAIGCRIAQTLMQYCVLANHYWFFGEAVYLYSVLIASVFIDNDKHLPYICVCWGTPLLFVIPWVVMKILKENKECWGFNENMNYWWIIRFPILFASLINFLIFMKILKVIFSKLRANNQKQIPKKYSATLTLIPLFGVHEIIFIFATDEQTTGVLRYIKVFFTLFLNSFQGFIVAVLYCYANKEVSKNRHRRHSPIKRHSLASEQMKCKFITLTTAGKKLNFSLVLALRLLSLLPEGKRANSPFAGWVGALIMHNAPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C679</accession>
    <name>A0A673C679_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transmembrane emp24 domain-containing protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tmed5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C679"/>
    <dbReference type="Ensembl" id="ENSSORT00005052172.1">
        <property type="protein sequence ID" value="ENSSORP00005050956.1"/>
        <property type="gene ID" value="ENSSORG00005023057.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C679"/>
    <dbReference type="OrthoDB" id="5976732at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="45"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="26174" checksum="E840A80642071784" modified="2020-06-17" version="1">MEVVRVFLCVLSVFVALLSERFVVLAAFSQSLDSDFTFSLPAGRKECFYQTMKKDASLEIEYQVLDGAGLDVDFYISSPSGQVLFSDYRKSDGVHTVETEDGDYMFCFDNTFSAVSEKIIFFELILDNMETDEDPDDWKEYVHGTDILDMKLEDIMDTINSVKARLGKSLQVQTVLRAFEARDRNLQESNFDRVNFWSIVNLIVMVCVSGVQVYLVRSLFEDKRKVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C745</accession>
    <name>A0A673C745_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Glypican 5b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115418666</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Lipid-anchor</topology>
            <topology evidence="1 12">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C745"/>
    <dbReference type="Ensembl" id="ENSSORT00005051013.1">
        <property type="protein sequence ID" value="ENSSORP00005049810.1"/>
        <property type="gene ID" value="ENSSORG00005022595.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C745"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905475">
        <property type="term" value="P:regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001863">
        <property type="entry name" value="Glypican"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822:SF19">
        <property type="entry name" value="GLYPICAN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01153">
        <property type="entry name" value="Glypican"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 12" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025389470" evidence="13">
        <location>
            <begin position="33"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="457" mass="50847" checksum="81B17271CC742F91" modified="2020-06-17" version="1">MFRAALRNADLFWVILFCVVFLPEAACGSADADSCHEVKTAYMMRQIGPVELVPDTPGTDESLRICVHPGPSCCTSKMEDSYMMAVRSETQQKMRSYSFELKYLIAGHTKAYQETFESLVSFTSNLTSTLFDSAYSALASDCRPLVFQLFSDIKRHLSGDANSSLDKTVRRFYNDLFPLVYRRLLNPGFGHMSPQSYSSLPSSHDDCLRMTRQDVSPFGPHPHLLVSSLSRALGVGRALSRLLRLAGEVVNATEKATLSRECGRGLVRMQYCSHCRGMTLIRPCTGLCVNVMRGCLVGVSELGEPWGSLVVLLQRLAATLATSSNHNSMELALLAVRNQVNDAILHAQLHGSFPLKGSKGDKSRSLKKLSREFQGSVQRYQWFFSELPEMLCESEMEVEQHTCWSGQDVVESYAGRVVGSSLKAQRENPEMTVRNTDPVLKGAKQKLEELTQVNITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCR5</accession>
    <name>A0A673CCR5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NDRG family member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ndrg2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCR5"/>
    <dbReference type="FunCoup" id="A0A673CCR5">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053108.1">
        <property type="protein sequence ID" value="ENSSORP00005051870.1"/>
        <property type="gene ID" value="ENSSORG00005023422.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCR5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="342" mass="37656" checksum="BE914346443ED3FF" modified="2020-06-17" version="1">MTTEMQEIAITEDKPLLTGQADTTKDAELAARILLDQGQEHSIETPHGVLHVTLHGARTTRRPAILTFHDVGLDSKSCFSTLFKFEEMQEIVKSFTLIHIDAPGQDEGAATYPTGFRTVIGVGVGAGAYILSKFTLANPDAVEGLVLVNIDTNARGWMDWAAQKLSSVTSSLTEQVLSHLFSQEELSSNTDLVQSHRDRISKSSNLFNIELFWKAYNSRRDMNLDRNSTFKCPVMLVVGDQAPYEDAAVECNSKLDPTTTSFLKVPHCHDFPAKLTEAFKYFIQGMGYMASSCMTRLSRSRTTSLSSSYSMDGSRSRSRTLSQGSQGGQMPPSPSQTMEVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CDS8</accession>
    <name>A0A673CDS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 23">Protein kinase C</fullName>
            <ecNumber evidence="5 23">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">LOC115420825</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="27">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="27">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="5 23"/>
    <dbReference type="AlphaFoldDB" id="A0A673CDS8"/>
    <dbReference type="Ensembl" id="ENSSORT00005052695.1">
        <property type="protein sequence ID" value="ENSSORP00005051464.1"/>
        <property type="gene ID" value="ENSSORG00005023185.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CDS8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20833">
        <property type="entry name" value="C1_cPKC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20836">
        <property type="entry name" value="C1_cPKC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04026">
        <property type="entry name" value="C2_PKC_alpha_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000012">
        <property type="entry name" value="Kinase C alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000023">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000080">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000006">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000031">
        <property type="entry name" value="Protein kinase C alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014375">
        <property type="entry name" value="Protein_kinase_C_a/b/g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000550">
        <property type="entry name" value="PKC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="18 23 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17 27" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 27" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
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            <begin position="34"/>
            <end position="84"/>
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            <position position="184"/>
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        <ligand>
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        <location>
            <position position="362"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CF78</accession>
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            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <sequence length="555" mass="61167" checksum="EE314E28DF1B6494" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFV5</accession>
    <name>A0A673CFV5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Activating transcription factor 7a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atf7a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CFV5"/>
    <dbReference type="Ensembl" id="ENSSORT00005052830.1">
        <property type="protein sequence ID" value="ENSSORP00005051597.1"/>
        <property type="gene ID" value="ENSSORG00005023302.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CFV5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000010">
        <property type="entry name" value="Cyclic AMP-dependent transcription factor ATF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016378">
        <property type="entry name" value="TF_CRE-BP1-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003153">
        <property type="entry name" value="ATF2_CRE-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="10">
        <location>
            <begin position="327"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="75"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="261"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="406"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="455"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="424"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR003153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="480" mass="51444" checksum="87CEB1E59055574E" modified="2020-06-17" version="1">MFSSSPLPQLHCKLIYVTYVFVFFFCSCHEMTLKFGPARTDSVIIADQTPTPTRFLKNCEEVGLFNELANSFEQDEDDKKLPTSNSVALDMTLQTSSDVKVKEESPVEVDSSPPDSPESISSRSDSTRESLVKGKVGQSICVSSAPTPTIVRPGSLPLHLGFDALQPTMPSPTSVITQAPPSNRTLGSPTSHYPMMMLPTGQAVPVLPGPVQMPSVINLARPVCMVPNIPGIPGPPLGGSSSGSNSPSGYIHSEAKMRLKAALSQQSPSGHSMGIMAMGSSPMVPQKAEQSQLLVQHPDAPSPAQPQVSPAQPTGGRRRRTADDDPDERRQRFLERNRAAASRCRQKRKLWVSSLEKKAEELSNLNVSLSNEVSLLRNEVAHLKQLLLAHKDCPVTTLQKKTAYLEESMKDSSEPTGSPAPVIQHSSLAPSPSSGQNGLNTRAAAEAMAMSVLAGMGQQQRAESGPSHIIMAAQSQSAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJY2</accession>
    <name>A0A673CJY2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Guanylate cyclase activator 2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the guanylin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJY2"/>
    <dbReference type="Ensembl" id="ENSSORT00005054845.1">
        <property type="protein sequence ID" value="ENSSORP00005053589.1"/>
        <property type="gene ID" value="ENSSORG00005024091.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJY2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030250">
        <property type="term" value="F:guanylate cyclase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1450.10">
        <property type="entry name" value="Guanylin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000879">
        <property type="entry name" value="Guanylin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036382">
        <property type="entry name" value="Guanylin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11318:SF4">
        <property type="entry name" value="GUANYLATE CYCLASE ACTIVATOR 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11318">
        <property type="entry name" value="GUANYLIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02058">
        <property type="entry name" value="Guanylin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00774">
        <property type="entry name" value="GUANYLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89890">
        <property type="entry name" value="Proguanylin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="90" mass="10193" checksum="D321B71C41A13204" modified="2020-06-17" version="1">ILPVTKCLCNTVLSCCFQVGDRSFPLEAVKQLKELMNMDDHISPHLAETRAASVCANPLLPLVFRPVCQGRERDQVLYKLAHPMRLIYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKH3</accession>
    <name>A0A673CKH3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-hydroxytryptamine receptor 1B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Serotonin receptor 1B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Homodimer. Heterodimer with HTR1D.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CKH3"/>
    <dbReference type="Ensembl" id="ENSSORT00005055981.1">
        <property type="protein sequence ID" value="ENSSORP00005054705.1"/>
        <property type="gene ID" value="ENSSORG00005024485.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKH3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046849">
        <property type="term" value="P:bone remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050795">
        <property type="term" value="P:regulation of behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002147">
        <property type="entry name" value="5HT1B_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1B"/>
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    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00513">
        <property type="entry name" value="5HT1BRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
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        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
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        </location>
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    </feature>
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    </feature>
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    </feature>
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            <begin position="35"/>
            <end position="336"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
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            <end position="239"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025985"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="358" mass="39820" checksum="ACF098BB0786A123" modified="2020-06-17" version="1">GSPFNHRGPQHEETLAYQISLAVVLSVITLATTLSNAFVIATISQSKKLQTPANFLIASLAITDLLVSILVMPICVLYTVIHTWTLGQIVCDIWLSSDITCCTASILHLCVIALDRYWAITDAVEYSKKRTPGRAAGMVATAWVIAISISMPPLFWRQVKAEELTSCSVNTDHIFYTIYSTFGAFYIPTLLLLVLYGRIYVEARKRILKQSPKKVGKRLTSAHLVTNSPGSVASTTSLQYGRHDTPSSDTGSSTSDSQVKVTVSDALLEKKRISAARERKATKTLGIILGAYIVCWLPFFIYTLVVATCDTCFLPELFDFFNWLGYLNSLINPIIYTMSNEDFKKAFHKLVRFRCCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKH6</accession>
    <name>A0A673CKH6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Tyrosine-protein phosphatase non-receptor type substrate 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115439033</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005052573.1"/>
        <property type="gene ID" value="ENSSORG00005023681.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439033"/>
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    <dbReference type="OrthoDB" id="6370831at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:immune system process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="33156" checksum="2006081D59BB15C3" modified="2020-06-17" version="1">MIKLYVLLISLLRVHGVFSYSVVYTHIGDNVSLPCIYASSARYLCWYKQAAGEQPQIISSDYIHSLHGNSEKFDGNKRFSVHAEAGSYHLKISNIQQSDSAIYYCGRTSVATTEFDNGTFLVLKESMHISFHQQKVSKPVQPGDSVTLNCTVHTGTSNKEHTVYWFREDSRSSGLGVMYVHTNSSSHCVKSLESGSATKRCMYSLTKRNVSLSDAGIYYCAVASCGEIVFGNGTRLDLEAQPQTSVPDYTADTQNEECDALQYVAVDFKKRQSKRRRHRSTEETTVYSSLRVSELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRC3</accession>
    <name>A0A673CRC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycoprotein hormones alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115413835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Shared alpha chain of heterodimeric glycoprotein hormones. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways. Involved in gametogenesis and steroidogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer. Glycoprotein hormones are heterodimers composed of a common alpha chain described here and a unique beta chain which confers their biological specificity to the different hormones.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycoprotein hormones subunit alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CRC3"/>
    <dbReference type="FunCoup" id="A0A673CRC3">
        <property type="interactions" value="1569"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057198.1">
        <property type="protein sequence ID" value="ENSSORP00005055909.1"/>
        <property type="gene ID" value="ENSSORG00005024916.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CRC3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016914">
        <property type="term" value="C:follicle-stimulating hormone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016913">
        <property type="term" value="F:follicle-stimulating hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010893">
        <property type="term" value="P:positive regulation of steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006590">
        <property type="term" value="P:thyroid hormone generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000011">
        <property type="entry name" value="Glycoprotein hormones alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000476">
        <property type="entry name" value="Glyco_hormone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11509">
        <property type="entry name" value="GLYCOPROTEIN HORMONE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11509:SF0">
        <property type="entry name" value="GLYCOPROTEIN HORMONES ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00236">
        <property type="entry name" value="Hormone_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00274">
        <property type="entry name" value="GLYCOHORMONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00067">
        <property type="entry name" value="GHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00779">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00780">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50277">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="131" mass="14664" checksum="EFFA665F8496BDB5" modified="2020-06-17" version="1">LKGNGPLIMVTTATMGSVKSAGLSVLLVSFLLYVANSYPNSDFSNMGCEECTLKKNFYFSRDRPIYQCMGCCFSRAYPTPLRARMTMMIPKNITSEATCCVAKHGYETEVDGIRVKNHTECHCSTCYYHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRM4</accession>
    <name>A0A673CRM4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein phosphatase 1 regulatory subunit 21</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Coiled-coil domain-containing protein 128</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ferry endosomal RAB5 effector complex subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">KLRAQ motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ppp1r21</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030011291.1">
        <property type="nucleotide sequence ID" value="XM_030155431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CRM4"/>
    <dbReference type="FunCoup" id="A0A673CRM4">
        <property type="interactions" value="747"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056480.1">
        <property type="protein sequence ID" value="ENSSORP00005055202.1"/>
        <property type="gene ID" value="ENSSORG00005024321.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433871"/>
    <dbReference type="CTD" id="129285"/>
    <dbReference type="InParanoid" id="A0A673CRM4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040024">
        <property type="entry name" value="PPP1R21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049372">
        <property type="entry name" value="PPP1R21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019343">
        <property type="entry name" value="PPP1R21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019348">
        <property type="entry name" value="PPP1R21_six_helix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21448:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21448">
        <property type="entry name" value="SMOOTH MUSCLE MYOSIN HEAVY CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10205">
        <property type="entry name" value="KLRAQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21636">
        <property type="entry name" value="PPP1R21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10212">
        <property type="entry name" value="PPP1R21_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01254">
        <property type="entry name" value="KLRAQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 21 N-terminal" evidence="11">
        <location>
            <begin position="12"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="614"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="131"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="558"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="701"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="616"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="628"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="785" mass="88847" checksum="0A11C0DE47C46C40" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSY1</accession>
    <name>A0A673CSY1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial-processing peptidase subunit beta</fullName>
            <ecNumber evidence="6">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pmpcb</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2. Required for PINK1 turnover by coupling PINK1 mitochondrial import and cleavage, which results in subsequent PINK1 proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of PMPCA (alpha) and PMPCB (beta) subunits, forming the mitochondrial processing protease (MPP) in which PMPCA is involved in substrate recognition and binding and PMPCB is the catalytic subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673CSY1"/>
    <dbReference type="Ensembl" id="ENSSORT00005056315.1">
        <property type="protein sequence ID" value="ENSSORP00005055038.1"/>
        <property type="gene ID" value="ENSSORG00005024575.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSY1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF103">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="18">
        <location>
            <begin position="83"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="19">
        <location>
            <begin position="236"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="504" mass="56091" checksum="742153580DDF705C" modified="2020-06-17" version="1">MAACLKNLTSIGRNVVQRHLLKTYSSPRVRICLRTCVGLKGFNGCCLHPQLTSGPHRLLAVQAPQVALNVPETKVTALENGLRVASEDSGLPTCTVGLWIDAGSRYENQRNNGTAHFLEHMAFKGTRKRSQLDLELEIENMGAHLNAYTSREQTVYYAKAFSKDLPRAVEILADIIQNSMLGEAEIERERGVILREMQEVETNLQEVVFDYLHATAYQSTALGRTILGPTENIKTINRGDLVEYITTHYKGPRIVLAAAGGVSHDELIDLAKYHFGKLPGRYEGEAPALPLCNFTGSEIRVRDDKMPLAHIAIAVEAVGWSHPDTIPLMVANTLIGNWDRSFGGGVNLSSKLAQMACQGNLCHSFQSFNTCYTDTGLWGLYMVCEPGTINDMMYFTQMEWMSLCTSVTESEVVRAKNLLKTNMLLHLDGSTPICEDIGRQMLCYSRRIPLHELEARIDAIDAKTIKEVCTKYIYNKAPAIAAVGPIEQLPDYNQIRSGMFWMKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSZ4</accession>
    <name>A0A673CSZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">scaper</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029991634.1">
        <property type="nucleotide sequence ID" value="XM_030135774.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056330.1">
        <property type="protein sequence ID" value="ENSSORP00005055053.1"/>
        <property type="gene ID" value="ENSSORG00005024415.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420503"/>
    <dbReference type="CTD" id="49855"/>
    <dbReference type="InParanoid" id="A0A673CSZ4"/>
    <dbReference type="OrthoDB" id="71500at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000680">
        <property type="entry name" value="S phase cyclin A-associated protein in the endoplasmic reticulum"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032446">
        <property type="entry name" value="SCAPER_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31434">
        <property type="entry name" value="S PHASE CYCLIN A-ASSOCIATED PROTEIN IN THE ENDOPLASMIC RETICULUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31434:SF2">
        <property type="entry name" value="S PHASE CYCLIN A-ASSOCIATED PROTEIN IN THE ENDOPLASMIC RETICULUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16501">
        <property type="entry name" value="SCAPER_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="2">
        <location>
            <begin position="885"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="798"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="845"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="951"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="845"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="963"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1489" mass="166325" checksum="57038EFBC791F822" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CVJ1</accession>
    <name>A0A673CVJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative nuclease HARBI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Harbinger transposase-derived nuclease</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transposase-derived protein that may have nuclease activity. Does not have transposase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CVJ1"/>
    <dbReference type="FunCoup" id="A0A673CVJ1">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059779.1">
        <property type="protein sequence ID" value="ENSSORP00005058455.1"/>
        <property type="gene ID" value="ENSSORG00005025796.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CVJ1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026103">
        <property type="entry name" value="HARBI1_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF267">
        <property type="entry name" value="NUCLEASE HARBI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02086">
        <property type="entry name" value="PUTNUCHARBI1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="13">
        <location>
            <begin position="119"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="290" mass="32302" checksum="F236F2408E8062CC" modified="2020-06-17" version="1">MFSDYKKNNMTTSYNAILELLDELGDNLGPVTRRSHAIPAMTKLLATLHFLASGSFQHTVTICAGISQSSFCSVVSEVLNTMSRRMGRHIQFPCTRDALNATKHGFYAVAGFPRVIGAIDCTHVQLVPPSDREHVYRNRKHTHSLNVQVVCNSHGVITNVVAKYPGSAHDSFILRNSVLFSKLRDGEYGDGWILGNSITSFCFGKKPKTFCEHCYNAAHIRTRNIVERTFSVLKSWFHCLDRTGGALLYSPEKVAQIMVVCCILHNIAKCHGMEHDAMDVEESEDLPQVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CXD8</accession>
    <name>A0A673CXD8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CXD8"/>
    <dbReference type="Ensembl" id="ENSSORT00005059637.1">
        <property type="protein sequence ID" value="ENSSORP00005058314.1"/>
        <property type="gene ID" value="ENSSORG00005025747.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059638.1">
        <property type="protein sequence ID" value="ENSSORP00005058315.1"/>
        <property type="gene ID" value="ENSSORG00005025748.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF106">
        <property type="entry name" value="CADHERIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="29"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="178" mass="19465" checksum="ACFF384420F551AD" modified="2020-06-17" version="1">MAGQVGGLSGSTTINITLTDVNDNPPKFPQKNYELYVVESAQVGKPVGKIKANDEDLGINADIKYSIINSEGANMFSISTDRDTREGIISLKKSLDYERKKTYTLHIEGTNTHVDPRFSYLGSFKDTATLKISVGDVDEPPVFSMDYYIMDVYENSPGGTEVGAVTARDPDSNNSPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CXP3</accession>
    <name>A0A673CXP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Syntaxin-8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">stx8</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993104.1">
        <property type="nucleotide sequence ID" value="XM_030137244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CXP3"/>
    <dbReference type="FunCoup" id="A0A673CXP3">
        <property type="interactions" value="690"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059727.1">
        <property type="protein sequence ID" value="ENSSORP00005058404.1"/>
        <property type="gene ID" value="ENSSORG00005025775.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421367"/>
    <dbReference type="CTD" id="9482"/>
    <dbReference type="InParanoid" id="A0A673CXP3"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15852">
        <property type="entry name" value="SNARE_Syntaxin8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000036">
        <property type="entry name" value="Putative Syntaxin-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041875">
        <property type="entry name" value="Syntaxin-8_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF124">
        <property type="entry name" value="SYNTAXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="148"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="238" mass="26432" checksum="C7800010AB29EC21" modified="2020-06-17" version="1">MSQDPWLQNYDATCRLAQEIAENIQERNRQQRTGGNPAKINMTLRASLQKLKQNIAQLKESLLRASSSRRVMQAEADRRQNLIDDLLTREKQLNATFKGDISEPEPARSTLMAGNAGASGGGAANPWLINETEETRGLTFGEIKQQQQRIIEAQDAGLDALAAVISRQKIMGQEIGNELDEHNEIIDDLAHLVDKTDDRIRNETRRVKLVETKSASCGMLVVIVLLLIAIVVVAAWPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CY32</accession>
    <name>A0A673CY32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Apolipoprotein M</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CY32"/>
    <dbReference type="Ensembl" id="ENSSORT00005059936.1">
        <property type="protein sequence ID" value="ENSSORP00005058609.1"/>
        <property type="gene ID" value="ENSSORG00005025854.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CY32"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11967">
        <property type="entry name" value="ALPHA-1-ACID GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11967:SF2">
        <property type="entry name" value="ALPHA-1-ACID GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025359535" description="Apolipoprotein M" evidence="5">
        <location>
            <begin position="20"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21411" checksum="6FAB1CF204E9E889" modified="2020-06-17" version="1">MASAGCVFLFLGLMSLSHCAPLPCEDLVRPKAQLYPHHLEGRWTLMALSLTHLPYPDTFNQTDGAFITFSSNSSANTVSYIRTIRLNNKCHSHSYNITLEGSTFTYDGWNSSNWTANFVQTSCHDCLIMHLRLEPAKQQQLFLLSRRTAVEQMEIEEFRAQAKCLKMPFNIPFLEVVLGFFSIVILQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CY53</accession>
    <name>A0A673CY53_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115423430</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Choline transporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>choline(out) + n H(+)(in) = choline(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75463"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ethanolamine(out) + n H(+)(in) = ethanolamine(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Cell membrane</location>
            <topology evidence="2 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CY53"/>
    <dbReference type="FunCoup" id="A0A673CY53">
        <property type="interactions" value="1115"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058330.1">
        <property type="protein sequence ID" value="ENSSORP00005057028.1"/>
        <property type="gene ID" value="ENSSORG00005025320.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CY53"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF34">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="460"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="508"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="608"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="643"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="709"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="710"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="728"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="740" mass="84013" checksum="DC65BE9DE961843A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y2V3</accession>
    <name>A0A672Y2V3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Immunoglobulin heavy variable 6-1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y2V3"/>
    <dbReference type="Ensembl" id="ENSSORT00005000468.1">
        <property type="protein sequence ID" value="ENSSORP00005000442.1"/>
        <property type="gene ID" value="ENSSORG00005000309.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y2V3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001532">
        <property type="entry name" value="Immunoglobulin heavy variable 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="166" mass="18779" checksum="A8CB21E12CC1AA6D" modified="2020-06-17" version="1">MSSFTCTDSFLEPIWRIPLTKCVDGQTLTESEPAVKRPGQSHRLTCTTSGFTFSSYPMSWIRQAPGKGLEWIAFIHTGSSHIYYSSSVQGRFTISRDDSSSKLYLQMNSLKSEDTAVYYCAREHTMTDNNRGSHTKTTSLFQTSSGTFIYNTDHNDYRLLLSVNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y321</accession>
    <name>A0A672Y321_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synaptogyrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">syngr2b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synaptogyrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y321"/>
    <dbReference type="Ensembl" id="ENSSORT00005000087.1">
        <property type="protein sequence ID" value="ENSSORP00005000078.1"/>
        <property type="gene ID" value="ENSSORG00005000064.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y321"/>
    <dbReference type="OrthoDB" id="10041611at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016579">
        <property type="entry name" value="Synaptogyrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838:SF33">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011282">
        <property type="entry name" value="Synaptogyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="24"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="219"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR011282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="247" mass="27199" checksum="18E72A30AAD13FE6" modified="2020-06-17" version="1">MEETGGATAYGASLAGGGFDFYKFVRQPQTIVRILSWVFAIVVFGCITTEGYINPPTSSHAKCIFNQNDSACQYAVSVGVLGFLACVGFLVLDVYLPFMSSAQERKYAVMADLGFSGVWTFLWFVCFCLLASQWARTHDVSGIPEDAARATVAFSFFSIATWGILTYFALVRFRRGVNEVTIPTYTEPTPEPHTPYPPTYAPPSYNPTTYTPTTYSVYPSSGPEMQPSPFTQNTQPQGDMGYQPPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3Y1</accession>
    <name>A0A672Y3Y1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Caspase recruitment domain-containing protein 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Bcl10-interacting CARD protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">card19</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Associates with BCL10 by CARD-CARD interaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y3Y1"/>
    <dbReference type="Ensembl" id="ENSSORT00005000976.1">
        <property type="protein sequence ID" value="ENSSORP00005000947.1"/>
        <property type="gene ID" value="ENSSORG00005000578.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y3Y1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000015">
        <property type="entry name" value="Caspase recruitment domain-containing protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043574">
        <property type="entry name" value="CARD19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34765">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34765:SF1">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="185" mass="21439" checksum="E5FED6B665250599" modified="2020-06-17" version="1">HYLLTWTDSFREQLTEDSAFLKADRRLDTELVDKVILQLNRIYPQVLTDKEATRFRNLDVPTSVRLGELLTHLRGKGEEACKEFYRALHLHVEEVYYSLPTRLRLRDSLDPLKHPQAYQQRYVLNDRGPVFFLGCFGFALGMALLYYYSESKVTGGSRALGMAALGLKKKAQEVLIWYNKGSLMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y530</accession>
    <name>A0A672Y530_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y530"/>
    <dbReference type="Ensembl" id="ENSSORT00005001557.1">
        <property type="protein sequence ID" value="ENSSORP00005001512.1"/>
        <property type="gene ID" value="ENSSORG00005000936.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y530"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF487">
        <property type="entry name" value="SOLUBLE SCAVENGER RECEPTOR CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="18"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="223" mass="24234" checksum="34943A5A19D3B801" modified="2020-06-17" version="1">TAEPVLLSLCLCSDPAGVRLVGQQNRCAGTLEIQHQGQWRPVGDRNKLWNLKSGSAVCQYLDCGSAVSVKRTDDSTFRPVWSVSVPCVKLTSGPRDCVGLDEPNYHFSGVDVVCSDLLPQPNISLSDGVFGVYQQGFWVLVDSDFTITCSVQPQYPGGSFQLISDTKKPLNLTLPAVNHSAHFLLSSMGYAHRGNYTCVYHVDVYNHSFSSSQSPALYLTVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5A5</accession>
    <name>A0A672Y5A5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">get3</name>
        <name evidence="8" type="synonym">ASNA1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997400.1">
        <property type="nucleotide sequence ID" value="XM_030141540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y5A5"/>
    <dbReference type="FunCoup" id="A0A672Y5A5">
        <property type="interactions" value="1969"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001670.1">
        <property type="protein sequence ID" value="ENSSORP00005001622.1"/>
        <property type="gene ID" value="ENSSORG00005001003.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424333"/>
    <dbReference type="CTD" id="439"/>
    <dbReference type="InParanoid" id="A0A672Y5A5"/>
    <dbReference type="OrthoDB" id="1770at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000235">
        <property type="entry name" value="ATPase ASNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="9">
        <location>
            <begin position="32"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="341" mass="38367" checksum="93FDAEFAF7BC9234" modified="2020-06-17" version="1">MAASLEDEFEDAPDVEPLEPTLKNIIEQKSLKWIFVGGKGGVGKTTCSCSLAVQLAAVRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCRIDTHNIIVNQLVFPDTERPCKMCEARHKIQSKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSKQLLEPYKPPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y661</accession>
    <name>A0A672Y661_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">klhl7</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030004053.1">
        <property type="nucleotide sequence ID" value="XM_030148193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y661"/>
    <dbReference type="Ensembl" id="ENSSORT00005002129.1">
        <property type="protein sequence ID" value="ENSSORP00005002072.1"/>
        <property type="gene ID" value="ENSSORG00005001267.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428929"/>
    <dbReference type="CTD" id="55975"/>
    <dbReference type="InParanoid" id="A0A672Y661"/>
    <dbReference type="OrthoDB" id="19132at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18237">
        <property type="entry name" value="BTB_POZ_KLHL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030599">
        <property type="entry name" value="BTB/POZ_KLHL7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF435">
        <property type="entry name" value="KELCH-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="47"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="592"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="597"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="614" mass="68353" checksum="99307BEA52784531" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y687</accession>
    <name>A0A672Y687_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="493" mass="50291" checksum="9DD21782039BF5D7" modified="2020-06-17" version="1">FLFAVCDPSAVCDPTVCDPSAVCDPSAVCDPTVCDPSAVCDPTVCDPSAVCDPTVCDPSAVCDPSAVCDPSAVCDPTVCDPSTVCDPSAVCDPTVCDPSAVCDPSAVCDPSAVCDPTVCDPSTVCDPSTVCDPSAVCDPTVCDPSAVCDPSAVCDPTVCDPSAVCDPSAVCDPSTVCDPSVCDPSTVCDPSVCDPSTVCDPSTVCDPSAVCDPTVCDPSAVCDPTVCHPTVCDPSAVCDPTVCHPTVCDPSAVCDPSVCDPSTVCDPSVCDPTVCDPSTVCDPSAVCDPSTVCDPSRVCDPSAVCDPSAVCDPSTVCDPSRVCDPSAVCDPSAVCDPTVCDPSALCDPSAVCDPSSVCDPSTVSSFPCFFLKKFSSEFCFWLISLACVSTDSGSGAPKSSCCEVREETVAFQNKISARQMGKIALILTTIFFLCGLVPNDPRTSTGPVTGGWDNCFRRTPHSAPTNNSRIPYVSLQSSNINASCPEMSNENDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6K9</accession>
    <name>A0A672Y6K9_9TELE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y6K9"/>
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        <property type="protein sequence ID" value="ENSSORP00005001968.1"/>
        <property type="gene ID" value="ENSSORG00005001218.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="141" mass="15967" checksum="E6F1575D9BD0A51F" modified="2020-06-17" version="1">DFGICFKAIHTSGGFLNDRLTPEVRFRPWATPLLGPIAPMVSSDDCRGLTGIPVTQITSAAAVISRSCYFDRADKKKHTSSNQSELVFLISNLLLLNISSWEEKSHHPFEELIHELDGERHHIHLMREELIIHILHNTECV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAV0</accession>
    <name>A0A672YAV0_9TELE</name>
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        <recommendedName>
            <fullName evidence="21">Palladin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">palld</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="20">Interacts with EPS8. Interacts with LASP1. Interacts with VASP. Interacts with ACTN. Interacts with SORBS2. Interacts with PFN1. Interacts with LPP. Interacts with SPIN90. Interacts with SRC. Interacts with EZR. Interacts with RAI14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
            <location evidence="2">Z line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the myotilin/palladin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
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    <dbReference type="FunCoup" id="A0A672YAV0">
        <property type="interactions" value="935"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001591.1">
        <property type="protein sequence ID" value="ENSSORP00005001544.1"/>
        <property type="gene ID" value="ENSSORG00005000338.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAV0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="IgI_Myotilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="myopalladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000147">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="palladin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="palladin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="279"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="426"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="886"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="1018"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="1117"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
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            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
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            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
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            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="559"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="617"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="864"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="981"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="563"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="628"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
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            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="692"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1000"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00065150"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00072991"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1262" mass="137516" checksum="D5956687E4E8D49F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDC8</accession>
    <name>A0A672YDC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tdrd12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A672YDC8"/>
    <dbReference type="Ensembl" id="ENSSORT00005000965.1">
        <property type="protein sequence ID" value="ENSSORP00005000936.1"/>
        <property type="gene ID" value="ENSSORG00005000571.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDC8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042078">
        <property type="term" value="P:germ-line stem cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20435">
        <property type="entry name" value="Tudor_TDRD12_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001416">
        <property type="entry name" value="Tudor domain containing 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22655">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22655:SF2">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Tudor" evidence="10">
        <location>
            <begin position="57"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="9">
        <location>
            <begin position="760"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="760"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1014" mass="113988" checksum="43D4397078E24C6B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YEP3</accession>
    <name>A0A672YEP3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable hydrolase PNKD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pnkd</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YEP3"/>
    <dbReference type="FunCoup" id="A0A672YEP3">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002981.1">
        <property type="protein sequence ID" value="ENSSORP00005002895.1"/>
        <property type="gene ID" value="ENSSORG00005001803.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YEP3"/>
    <dbReference type="OrthoDB" id="449487at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF116">
        <property type="entry name" value="HYDROLASE PNKD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="103"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="359" mass="40524" checksum="57DAFFFA9F02B7CC" modified="2020-06-17" version="1">MALPERLLTLAASASVFCLLCVCFRFRRTGQAIWRKLLSKIMARTEKPLFRIAYTLYTRTRLGYMYYKRQMRKAREHYPAGHSTAQPVEFNGIKIIPISVLSDNYSYLIVDTASSVAVVVDPADPQSVQAVLEEEGVMLEAILCTHKHWDHSGGNKGLKRLHSSCRVYGNAADNIPGLTHPLSHRDSVNIGRMTFKALFTPGHTVGHMIYLLDGRAVGAPSSLFSGDLVFLSGCGRMFEGSATTMLSSLDTVGSLSDDTLLWPGHEYAEDNLLFAAEVEPHNAARENKYQWVLQQRGQKLCTSPSTIGEEKEYNPFLRSHSAELHLALGLQQFQDEDWTRFRARVLEELRKRKDLYNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YG16</accession>
    <name>A0A672YG16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="25">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">nme4</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Major role in the synthesis of nucleoside triphosphates other than ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell projection</location>
            <location evidence="9">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Mitochondrion intermembrane space</location>
            <topology evidence="22">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 23 24">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="25"/>
    <dbReference type="AlphaFoldDB" id="A0A672YG16"/>
    <dbReference type="Ensembl" id="ENSSORT00005003609.1">
        <property type="protein sequence ID" value="ENSSORP00005003508.1"/>
        <property type="gene ID" value="ENSSORG00005001456.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YG16"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:CTP biosynthetic process"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:GTP biosynthetic process"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:UTP biosynthetic process"/>
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        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
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    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="21" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="14 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 25" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="26">
        <location>
            <begin position="36"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="23">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="23">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="23">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    <feature type="binding site" evidence="23">
        <location>
            <position position="137"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="147"/>
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        <ligand>
            <name>ATP</name>
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    <evidence type="ECO:0000256" key="1">
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        <source>
            <dbReference type="ARBA" id="ARBA00000937"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00003465"/>
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    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023306"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
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        <source>
            <dbReference type="RuleBase" id="RU004011"/>
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        <source>
            <dbReference type="RuleBase" id="RU004013"/>
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            <dbReference type="SMART" id="SM00562"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003508.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="185" mass="20598" checksum="1CB481A03F1DA560" modified="2020-06-17" version="1">SCLQTVHFCADVCASSLVSRHTPLSCLFPSDLAGVRERTLIAVKPDGVQRRLVGQIIQRFELRGFKLVGLKMLQVSEDLLSRHYSQLTGKPFYPDLVKYMTSGPVVVMVWEGYNAVQTSRAMVGLTNPADAQAGTVRGDFSIHVSRNVVHASDSPEGAQKEIQLWFQGQELLNWDCCYHNITCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIM9</accession>
    <name>A0A672YIM9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Far upstream element-binding protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">khsrp</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YIM9"/>
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        <property type="protein sequence ID" value="ENSSORP00005004409.1"/>
        <property type="gene ID" value="ENSSORG00005002672.1"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22482">
        <property type="entry name" value="KH-I_FUBP2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22488">
        <property type="entry name" value="KH-I_FUBP2_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000007">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="FUBP_C"/>
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        <property type="entry name" value="KH-I_FUBP2_rpt2"/>
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    <dbReference type="InterPro" id="IPR047371">
        <property type="entry name" value="KH-I_FUBP2_rpt4"/>
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    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09005">
        <property type="entry name" value="FUBP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="97"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="177"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="273"/>
            <end position="343"/>
        </location>
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        <location>
            <begin position="380"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="348"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="455"/>
            <end position="497"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="568"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="583"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="470"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="652" mass="67200" checksum="41D4DE96BD5B097F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJK6</accession>
    <name>A0A672YJK6_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJK6"/>
    <dbReference type="Ensembl" id="ENSSORT00005004848.1">
        <property type="protein sequence ID" value="ENSSORP00005004712.1"/>
        <property type="gene ID" value="ENSSORG00005002865.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJK6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051778">
        <property type="entry name" value="KHDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19447:SF15">
        <property type="entry name" value="KH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19447">
        <property type="entry name" value="OOCYTE-EXPRESSED PROTEIN HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="619" mass="72670" checksum="161E5F773828ACF7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJP8</accession>
    <name>A0A672YJP8_9TELE</name>
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        <recommendedName>
            <fullName evidence="10">Tripartite motif containing 35-12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJP8"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="206"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="46562" checksum="9A354104CDE7CFD8" modified="2020-06-17" version="1">MAEKKIELFESFLSCHVCSETFRDPVSLSCNHSFCSNCLKEFWEKTKNKNCPICKRKSSKDDPNMNFSLKELADLFAGRLNVGSSETKKGEKKVEVVCSKHQEEPKLFSEFNKLHQFLKEEEESRLAALREEEEQKRRSVIREMKRIEEQMSSVSDSICAVEEELQKASAPFLISCKDTQSRARAQCSVSEPQLISGALIHVDKHLGNLSFRVWDKMRDKVHFSPVILDPNTAGGWLHLSDDLTSVRRGDTYQHPPDNPERNTEYASVHGSEGFNSGKHSWDVEVGDHPEWILGLVKESADRKGETAVSPEYGFWCLWYVSGKYSNGAGQTLTVQTSPQRIRVELDYDRGEVSFYNAEDKTHIYTHTDTFTEKLYPYFNVRKAGDAETTDIRICQSKISVTYDMTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPP8</accession>
    <name>A0A672YPP8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flotillin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Heterooligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPP8"/>
    <dbReference type="Ensembl" id="ENSSORT00005006823.1">
        <property type="protein sequence ID" value="ENSSORP00005006564.1"/>
        <property type="gene ID" value="ENSSORG00005003846.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPP8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016600">
        <property type="term" value="C:flotillin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901890">
        <property type="term" value="P:positive regulation of cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000049">
        <property type="term" value="P:positive regulation of cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045807">
        <property type="term" value="P:positive regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070528">
        <property type="term" value="P:protein kinase C signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002090">
        <property type="term" value="P:regulation of receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03399">
        <property type="entry name" value="SPFH_flotillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806:SF33">
        <property type="entry name" value="FLOTILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FLOTILLIN-RELATED"/>
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            <begin position="72"/>
            <end position="232"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU366054"/>
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    </evidence>
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            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="369" mass="41546" checksum="914A359437B8BCE4" modified="2020-06-17" version="1">MFYTCGPNEAMVVSGFCRSPPLMIAGGRVFVFPCVQQIQSDKVYTRHGVPISVTEAAQMKIQGQNKQMLAAACQMFMGKSEAEIAQIALETLEGHQRAIIAHLTVEEIYKDRKKFSEQVFKVASSDLVNMGISVVSYTLKDVHDDQDYLHSLDARIGEAQNKRDAVIRVRYSTQLYRHGQAQRDYELKKAAYDIEVNTKKAESEMAYPQRRNNASRRKKMQVQVVGATQQITLQEKEITRREKELEAKVKKPAEAERYRLEKLAEAQRLKLIMEAEAEAESIKVVWKGEAEAFAVEARGRAEAEQMAKKAEAFQQYKDGAMVDMLLEKLPLMAEEISKPLCEANKVTMVSSGGGEVGAAKTVREDPWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQV5</accession>
    <name>A0A672YQV5_9TELE</name>
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        <submittedName>
            <fullName evidence="6">Flocculation protein FLO11-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115428275</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030003034.1">
        <property type="nucleotide sequence ID" value="XM_030147174.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005006974.1"/>
        <property type="gene ID" value="ENSSORG00005004031.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428275"/>
    <dbReference type="CTD" id="553292"/>
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    <dbReference type="OrthoDB" id="5792673at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
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    </dbReference>
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        <property type="term" value="P:heterochromatin organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF5604"/>
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        <property type="entry name" value="SETDB_methyltransferase"/>
    </dbReference>
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        <property type="entry name" value="Tudor_4"/>
    </dbReference>
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        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EGGLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46024:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EGGLESS"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="486"/>
            <end position="536"/>
        </location>
    </feature>
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        <location>
            <begin position="635"/>
            <end position="680"/>
        </location>
    </feature>
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        <location>
            <begin position="96"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="196"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="206"/>
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        </location>
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        <location>
            <begin position="226"/>
            <end position="272"/>
        </location>
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        <location>
            <begin position="282"/>
            <end position="384"/>
        </location>
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        <location>
            <begin position="398"/>
            <end position="438"/>
        </location>
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        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="700" mass="76446" checksum="52185534FF22DB4E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSF4</accession>
    <name>A0A672YSF4_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YSF4"/>
    <dbReference type="Ensembl" id="ENSSORT00005007801.1">
        <property type="protein sequence ID" value="ENSSORP00005007521.1"/>
        <property type="gene ID" value="ENSSORG00005004286.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YSF4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF151">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
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        <property type="match status" value="8"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="8">
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        <location>
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            <begin position="1"/>
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    </feature>
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        <location>
            <begin position="43"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="336" mass="37979" checksum="96CADB376AF0DAAA" modified="2025-10-08" version="2">MSFQTLYGTPDVSFPAPQIKTEAESEVGVEVGGAAGPAPEAQSETEDSEENWGGEPGLYNQDQIAEEDQNQGALWSSQPPGFGCRVCGESFYRRSSLMTHASAHLRSCAICGKQLEHGVSLKLHLRVHRETAFRCGVCGQSFSQHGNLRTHMRIHSGERPYGCTICGKSFGRRATLQRHIRSHTGEKPFTCTHCGRGFVEKGNLTVHLRIHTGERPYWCSTCDRRFNQLSSFTKHPCQRRRSTHTHTHTHTPFPPKRTWCLFGAGPLPFYNIDNTDKSFVCDICGKRLTRFDGYQKHLRVHTGEKPYTCHECGRRFSDNSNYKRHIRTHTGQKTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTA6</accession>
    <name>A0A672YTA6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Receptor-transporting protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115428955</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YTA6"/>
    <dbReference type="Ensembl" id="ENSSORT00005008020.1">
        <property type="protein sequence ID" value="ENSSORP00005007737.1"/>
        <property type="gene ID" value="ENSSORG00005004383.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTA6"/>
    <dbReference type="OrthoDB" id="8121437at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031849">
        <property type="term" value="F:olfactory receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026096">
        <property type="entry name" value="R-trans_p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027377">
        <property type="entry name" value="ZAR1/RTP1-5-like_Znf-3CxxC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14402">
        <property type="entry name" value="RECEPTOR TRANSPORTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14402:SF20">
        <property type="entry name" value="RECEPTOR-TRANSPORTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13695">
        <property type="entry name" value="Zn_ribbon_3CxxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01328">
        <property type="entry name" value="zf-3CxxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="3CxxC-type" evidence="9">
        <location>
            <begin position="84"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="221" mass="25644" checksum="E204C568FEB25A96" modified="2020-06-17" version="1">MGKTLRRCTSKEKKAYKSRSKGKNSQKSGAEWLPSLWQDTFEKLLLRDKELDYGDHWTIDFSYIQTNTLSLEERRGGWKVSCHCTNGKFQCASCQRMWPSARVQVLFRYRLWTGQGWGTVIMRPFGQSCHYCRNDVFEFCGFLKRDVKQTLRRLFAKIRKNCYGEEDGEVSYSLPVCAPKAKAKPHESSLCEACDMGICSQQDRSRTFWGTAAQNVNKEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTL8</accession>
    <name>A0A672YTL8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Retinol dehydrogenase 14-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:dkey-174n20.1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YTL8"/>
    <dbReference type="Ensembl" id="ENSSORT00005008244.1">
        <property type="protein sequence ID" value="ENSSORP00005007961.1"/>
        <property type="gene ID" value="ENSSORG00005004462.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTL8"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF64">
        <property type="entry name" value="RETINOL DEHYDROGENASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="35185" checksum="8EDB2CD35225BA0A" modified="2020-06-17" version="1">MYVFYTILASLVTFFILKWMKKRRYCTDLKRLDGKTVLITGGNSGIGKETAISLAMRGARVIIACRDMDKAEKAVRDIKFKSHSLNVLHMELDLANLRSVREFCKNFLQSEKRLDILVNNAGMPSVLDWTDDGFSMCFGVNHLGHFLLTNLLLPRLKECAPSRVVTLTCSSYKYQKLDFQDLNYNLLPFFTYCRSKLANIYFTHELERITEGKGVSTFAVHPGYVQSGWTCHYSFLFRMLMQVIMWMFFVSSEMGAQTVIYCAVSDEAAKHSGGYFVDCRPATLRPFARDAGVAKKLWEASERLVKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YV82</accession>
    <name>A0A672YV82_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115427831</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002384.1">
        <property type="nucleotide sequence ID" value="XM_030146524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YV82"/>
    <dbReference type="FunCoup" id="A0A672YV82">
        <property type="interactions" value="574"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008866.1">
        <property type="protein sequence ID" value="ENSSORP00005008566.1"/>
        <property type="gene ID" value="ENSSORG00005004748.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427831"/>
    <dbReference type="InParanoid" id="A0A672YV82"/>
    <dbReference type="OrthoDB" id="8881129at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00213">
        <property type="entry name" value="S-100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000044">
        <property type="entry name" value="Protein S100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034325">
        <property type="entry name" value="S-100_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF118">
        <property type="entry name" value="PROTEIN S100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="93" mass="10609" checksum="21ED450EF0994021" modified="2020-06-17" version="1">MSDTMNAMTLLIEVFNKHSGKEGDKTTLNKAELKELIQAEFGDLLGKANDKAALDRIFQDLDRNKDNSVDFNEFITLICCLTQMCHEYFTSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZD1</accession>
    <name>A0A672YZD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tuftelin 1a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tuft1b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YZD1"/>
    <dbReference type="Ensembl" id="ENSSORT00005010452.1">
        <property type="protein sequence ID" value="ENSSORP00005010121.1"/>
        <property type="gene ID" value="ENSSORG00005005504.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZD1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051375">
        <property type="entry name" value="Tuftelin_GRINL1A/MYZAP/CCD68"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23171">
        <property type="entry name" value="GDOWN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23171:SF17">
        <property type="entry name" value="TUFTELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="171"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="45023" checksum="D01CF550C1F7C6EC" modified="2020-06-17" version="1">MEEMSGGATRTVKHEDKKDTCKQLRLTPPDQNQSGNTSERHSTKESSIAVVLPQKPADQERTVELITPTEEKVEVIKVYLETRPDTTETHQNNLRMLTDEVSQIQEVRYCLKTLREQMAARQNSQNNNKYPANGYRVNMPSIQPAVTNGNTVLIESDANVGDNQEETVRLREATKRLYAQLKEMEKRHQEERERLQAENNKYRARLTEQSERLEKAEEQSKERGQQLEELQRLLGSMEIESSILKDKMAAGEEELLQLKAEREEGQEGEQRCAELEQEVATLKEKIHHLDDMLKSQQRKVRHMIEQLQNSRTVLQERDRVIGDLEERVAFLEAENREMHDHLEYLMAGQDPPPLTITENKPEVVYSKTLTPTTQSNKSLPFIKVIEIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0S1</accession>
    <name>A0A672Z0S1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z0S1"/>
    <dbReference type="Ensembl" id="ENSSORT00005010792.1">
        <property type="protein sequence ID" value="ENSSORP00005010455.1"/>
        <property type="gene ID" value="ENSSORG00005005648.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0S1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042974">
        <property type="term" value="F:nuclear retinoic acid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046966">
        <property type="term" value="F:nuclear thyroid hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042809">
        <property type="term" value="F:nuclear vitamin D receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097067">
        <property type="term" value="P:cellular response to thyroid hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051999">
        <property type="entry name" value="Mediator_complex_subunit_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="11">
        <location>
            <begin position="43"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="11">
        <location>
            <begin position="191"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="369" mass="41359" checksum="48BED8B5D1D46F41" modified="2020-06-17" version="1">LQFKKMKSIISGLHMKFAERSWNETFQLVRRCMVIYRFLPFASRLEMIAKQQGLGFHITDASCYLTADLFYLEAVLMPCGGVEEVKVALHGGLPVVSELFYCLLTAFRSKDFPKFSMKLGDLFNQYNIPGDKYTLIRSALFHVSCLMPVSHFNFFFFFFYLKPLVQAAQVTVGLRDETHQLQMASVILQPPQLDAHGSPVFKALDQTPCEPLPACFLLKLQPPIPMMLAFVHKLGQITGLPTSVNGVFLFFSHSLSKYSVNASTSMQPVPLPGGVMHRYVFPGAAWEAPARQGAVVDNIPFTHPSHVPAILDLLRHQCAINTLLTSLGTSHCENPGIIGHMRVKRSPFFFSFLCLKIPGVHSINTKYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2G4</accession>
    <name>A0A672Z2G4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Endoribonuclease ZC3H12A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115436461</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030015214.1">
        <property type="nucleotide sequence ID" value="XM_030159354.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030015215.1">
        <property type="nucleotide sequence ID" value="XM_030159355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z2G4"/>
    <dbReference type="Ensembl" id="ENSSORT00005011364.1">
        <property type="protein sequence ID" value="ENSSORP00005010990.1"/>
        <property type="gene ID" value="ENSSORG00005005941.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436461"/>
    <dbReference type="CTD" id="798235"/>
    <dbReference type="InParanoid" id="A0A672Z2G4"/>
    <dbReference type="OrthoDB" id="392925at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18729">
        <property type="entry name" value="PIN_Zc3h12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040546">
        <property type="entry name" value="Rege-1_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040757">
        <property type="entry name" value="Regnase_1/ZC3H12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF10">
        <property type="entry name" value="ENDORIBONUCLEASE ZC3H12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18561">
        <property type="entry name" value="Regnase_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18039">
        <property type="entry name" value="UBA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="472"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="377"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="431"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="485"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="624" mass="70376" checksum="0BFF50CB558DE3AA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z398</accession>
    <name>A0A672Z398_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nicotinamide mononucleotide adenylyltransferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nicotinate-nucleotide adenylyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z398"/>
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        <property type="protein sequence ID" value="ENSSORP00005011017.1"/>
        <property type="gene ID" value="ENSSORG00005005948.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z398"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:NAD+ biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
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        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF18">
        <property type="entry name" value="NICOTINAMIDE_NICOTINIC ACID MONONUCLEOTIDE ADENYLYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="15"/>
            <end position="62"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004594"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="92" mass="10608" checksum="33C6F8A373E37A32" modified="2020-06-17" version="1">MKNKSTKTHVILLSCGSFNPITKGHIHMFEKAREYLHKTGRFIVIGGIISPVHDSYGKPTRSHQDLINRQLIFIRTDKNETHQVQTERPITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3W1</accession>
    <name>A0A672Z3W1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115431832</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.</text>
    </comment>
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    <dbReference type="FunCoup" id="A0A672Z3W1">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011872.1">
        <property type="protein sequence ID" value="ENSSORP00005011490.1"/>
        <property type="gene ID" value="ENSSORG00005006144.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3W1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000067">
        <property type="entry name" value="Olfactory receptor C family, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000004">
        <property type="entry name" value="Taste receptor type 1 member 3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF418">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCQ19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025663361" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="25"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="632"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="667"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="712"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="752"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="792"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="824"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="597"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="864" mass="96246" checksum="28491FB7A27816F6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5M3</accession>
    <name>A0A672Z5M3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z5M3"/>
    <dbReference type="Ensembl" id="ENSSORT00005012498.1">
        <property type="protein sequence ID" value="ENSSORP00005012104.1"/>
        <property type="gene ID" value="ENSSORG00005006412.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z5M3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050795">
        <property type="term" value="P:regulation of behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046883">
        <property type="term" value="P:regulation of hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000248">
        <property type="entry name" value="5-hydroxytryptamine receptor 1A-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000610">
        <property type="entry name" value="5HT1A_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF191">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00512">
        <property type="entry name" value="5HT1ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0085">Behavior</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="30"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="397" mass="44728" checksum="C4B730DC051DA2B4" modified="2020-06-17" version="1">WNKDEEQVELGYQVLTSFLLGALILCAIFGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNRWTLGQVPCDIFVSLDVLCCTSSILHLCAIALDRYWAITEPIDYMKKRTPRRAAVLISVTWLVGFSISVPPMLIMRSQPRSVAEDRANPTQCQISTDPWYTIYSTFGAFYIPLTLMLVLYGRIFKAARFRIRRTVRKTEKKKVSDSCLALSPALFHKKPTAEAHGKSWRRTAEPRVLPLSVNGAVKHAEDGESLEIIEVHGGSRGNLPLPDTPGSAPLFQNRHEKATEAKRKIALARERKTVKTLGIIMGTFILCWLPFFIVALVKPFCPDSRFMPRWLEDVINWLGYSNSLLNPIIYAYFNKDFQSAFNKIIKCHFCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7S4</accession>
    <name>A0A672Z7S4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MyTH4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z7S4"/>
    <dbReference type="Ensembl" id="ENSSORT00005012924.1">
        <property type="protein sequence ID" value="ENSSORP00005012527.1"/>
        <property type="gene ID" value="ENSSORG00005006578.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z7S4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF21">
        <property type="entry name" value="MYOSIN XVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="MyTH4" evidence="5">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="365" mass="42007" checksum="32270BEF30AB5A95" modified="2020-06-17" version="1">MRFMGDAPLKGTAEHEVVGSFLKVKKHTHSFSVSEYFLLLIGEFTLMRDEAYCQLLKQLTANTSSKPDSCQRGWRLLYILTAFHRCSEVLKPFLLKYLQQASRSAGAQYQGIAKACEQNLKKTFQYGGRVVSPNSMELKAMMAGRSSKRQLFLFPGGVERHVKIKTCSVALEVIEELCYEMGLHRLEAMEEYAVFLVTNRGQNVRPLNKHEYILDVATEAELVDTNYSFWFRRVVWTQQLKFDNELCVAMHYNQILPDYRKGLLQILPHGKVSDQQFHQISKLAALQHKAKDIVFIPSVHELSEYIAAALFKMQPPQQWVNMVTQHMQQVQTLSPHQARAQFLGFYHGCRQTKQTKTQTGTNLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z819</accession>
    <name>A0A672Z819_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z819"/>
    <dbReference type="Ensembl" id="ENSSORT00005013085.1">
        <property type="protein sequence ID" value="ENSSORP00005012688.1"/>
        <property type="gene ID" value="ENSSORG00005006638.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z819"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="457"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="585"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="662" mass="73073" checksum="1AEB4687889B3424" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9F2</accession>
    <name>A0A672Z9F2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sema4ab</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z9F2"/>
    <dbReference type="Ensembl" id="ENSSORT00005014405.1">
        <property type="protein sequence ID" value="ENSSORP00005013986.1"/>
        <property type="gene ID" value="ENSSORG00005007165.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9F2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045791">
        <property type="entry name" value="Sema4F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF145">
        <property type="entry name" value="SEMAPHORIN-4A ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19428">
        <property type="entry name" value="Sema4F_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="697"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="10">
        <location>
            <begin position="24"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="525"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="605"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="726"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="759"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="605"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="789" mass="87989" checksum="A71FA0F350D5E42C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZA33</accession>
    <name>A0A672ZA33_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">snx10b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZA33"/>
    <dbReference type="FunCoup" id="A0A672ZA33">
        <property type="interactions" value="591"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014623.1">
        <property type="protein sequence ID" value="ENSSORP00005014201.1"/>
        <property type="gene ID" value="ENSSORG00005007276.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZA33"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06898">
        <property type="entry name" value="PX_SNX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043544">
        <property type="entry name" value="SNX10/11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46209">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46209:SF4">
        <property type="entry name" value="SORTING NEXIN-10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="11">
        <location>
            <begin position="18"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="225" mass="25915" checksum="3BC31AC4DF6327A6" modified="2020-06-17" version="1">MNIRIMTEGGDHKANQQIISVWVRDPRIRKNDFWHAYMDYEICLHTNSVCFTKKISSVRRRYSEFVWLRQKLQENSLLMVKLPELPPKNPFFSLNNAKQISERMQGLQKFLEKILQSPLLLSDSCLHLFLQSQLSVSKMEACAAGRTHYSVTQAVQRCGLRRFHSEEDLQKDLNLSCDFDSDSSESRDAEPRIKDLALTKSESAPLFDFVGTSQEETLSSSFGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZA54</accession>
    <name>A0A672ZA54_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZA54"/>
    <dbReference type="Ensembl" id="ENSSORT00005014643.1">
        <property type="protein sequence ID" value="ENSSORP00005014221.1"/>
        <property type="gene ID" value="ENSSORG00005007287.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZA54"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17679" checksum="82BFFBB1163852A1" modified="2020-06-17" version="1">NYRSQWIYDHTRHLVTGRILTSISYNVITLFPIIFFHVKCDLSLIDKVHISVMWRELYNNKLIVHKYGSGQPDEQDPIYTNRTEMKKHWLRTGDFSVTLKHPTDFDTNGYVCIVYNKNKVLRKKQVVLERPCAHITSTPFYIVPCVIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCV1</accession>
    <name>A0A672ZCV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZCV1"/>
    <dbReference type="Ensembl" id="ENSSORT00005015189.1">
        <property type="protein sequence ID" value="ENSSORP00005014760.1"/>
        <property type="gene ID" value="ENSSORG00005007520.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZCV1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15534">
        <property type="entry name" value="PHD2_PHF12_Rco1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000154">
        <property type="entry name" value="PHD finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.60">
        <property type="entry name" value="PHD finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042163">
        <property type="entry name" value="PHF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031966">
        <property type="entry name" value="PHF12_MRG-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038098">
        <property type="entry name" value="PHF12_MRG-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309:SF1">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16737">
        <property type="entry name" value="PHF12_MRG_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="311"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="523"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="562" mass="61289" checksum="D68D1ED7A68FEDCB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZG36</accession>
    <name>A0A672ZG36_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="11">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pygma</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZG36"/>
    <dbReference type="FunCoup" id="A0A672ZG36">
        <property type="interactions" value="760"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016280.1">
        <property type="protein sequence ID" value="ENSSORP00005015784.1"/>
        <property type="gene ID" value="ENSSORG00005007998.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZG36"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04300">
        <property type="entry name" value="GT35_Glycogen_Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000005">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000153">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000197">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF11">
        <property type="entry name" value="ALPHA-1,4 GLUCAN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="858" mass="98956" checksum="E3A1B6214E53FFDD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGB6</accession>
    <name>A0A672ZGB6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gamma-1-syntrophin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Syntrophin-4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">sntg1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Adapter protein that binds to and probably organizes the subcellular localization of a variety of proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. May participate in regulating the subcellular location of diacylglycerol kinase-zeta to ensure that diacylglycerol is rapidly inactivated following receptor activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the syntrophin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZGB6"/>
    <dbReference type="Ensembl" id="ENSSORT00005016015.1">
        <property type="protein sequence ID" value="ENSSORP00005015523.1"/>
        <property type="gene ID" value="ENSSORG00005007875.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGB6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016013">
        <property type="term" value="C:syntrophin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06801">
        <property type="entry name" value="PDZ_syntrophin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000448">
        <property type="entry name" value="Syntrophin gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000080">
        <property type="entry name" value="Syntrophin gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015482">
        <property type="entry name" value="Syntrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055108">
        <property type="entry name" value="Syntrophin_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554:SF2">
        <property type="entry name" value="GAMMA-1-SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10554">
        <property type="entry name" value="SYNTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23012">
        <property type="entry name" value="Syntrophin_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="12">
        <location>
            <begin position="41"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="267"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="509" mass="57200" checksum="2AC8D91D4E80A031" modified="2020-06-17" version="1">MQEGNEEPFNIRLHLAKDILTIQEQDVICVSGEPFYSSERTVTIRRQTIGGFGLSIKGGAEHKIPVVISKISKEQKAELSGLLFIGDGILQINGINVRSYRHEEVVQVLRNAGEEVTLTVSFLKKTPAFLKLPLCEDCTCIPSDQSSGTSSPLCDSGLHLNYHPNNTDTLSCSSWPTSPGLRWEKRWVDLRLIPLLHSRLSQYIPGSDVCRKNAFQVIAVDGVCSGVIQFPTAEDCLDWLQAIASNISSLTKHNVKKINRNFPVNQQIIYMGWVDEKEQDSVQDRMYSPKFLALRGSSLFKFAAPPVTTWDWTRAEKSFTVYEIMCKILKDNDLLDKRKHCFSVQTETGEDMFFSVELECELLIWEKAFQMATFLEVERIQCKTYACIMESHLMGLTVDFSMGFVCFDAASKAVLWRYKFSQLKGSSDDGKSKIKFLFQNQDSKSIEAKELEFSNLFAVLHCIHAFFAAKVACLDPMFVESQGTATTAGIPSLSSISCNSQTPKLKYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJ49</accession>
    <name>A0A672ZJ49_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">trypsin</fullName>
            <ecNumber evidence="8">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115425197</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZJ49"/>
    <dbReference type="FunCoup" id="A0A672ZJ49">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017017.1">
        <property type="protein sequence ID" value="ENSSORP00005016507.1"/>
        <property type="gene ID" value="ENSSORG00005008079.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJ49"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000005">
        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF52">
        <property type="entry name" value="GRANZYME K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="41"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="258" mass="28648" checksum="C698936EDAF410BF" modified="2020-06-17" version="1">KCSLDILLPHPVKRKKKSINLNIYINIELEQKLNANHGAEIIGGTEVKPHSMPFMALLENKDPVCGGILIHPKWVLTAAHCDKSVSKVLLGVHSIKEKEKDSRQIRIVKNSIPHPCYDKEEKVNDLRLLKLDKPVKQTKTVQCLSLPKSSRDPAGKSRCVVAGWGKTKNNAIKGSDVLRSVNVTVIDRRTCNSQDYYNLKPFITGDMICGDSGGPLLCNGVLTGVASFGYKCGLIKKPGVYAYLSDRQLSWIKKTIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZM28</accession>
    <name>A0A672ZM28_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZM28"/>
    <dbReference type="Ensembl" id="ENSSORT00005018016.1">
        <property type="protein sequence ID" value="ENSSORP00005017497.1"/>
        <property type="gene ID" value="ENSSORG00005008741.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZM28"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="166" mass="18572" checksum="3D9C4B1B7989FC8E" modified="2020-06-17" version="1">WDSAMSARSSASSSSLVFVVTFGQMLIRFSYPGFQFLQLLLATLHGQVLSLIQTVLQVLHSNLQVLLHPLQMSASVLLLLQLLSHHGGLHRIKCMGIIPAPDLRVQSALHGVNHPLAVPLDLLHLFIFLCQLPVHLTLNLVELQLDTQDLRLFMFQSSLNKSRHKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQH1</accession>
    <name>A0A672ZQH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">12S rRNA N(4)-cytidine methyltransferase METTL15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Methyltransferase 5 domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Methyltransferase-like protein 15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mettl15</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">N4-methylcytidine (m4C) methyltransferase responsible for the methylation of position C839 in mitochondrial 12S rRNA. Involved in the stabilization of 12S rRNA folding, therefore facilitating the assembly of the mitochondrial small ribosomal subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine(839) in 12S rRNA + S-adenosyl-L-methionine = N(4)-methylcytidine(839) in 12S rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62524"/>
            <dbReference type="Rhea" id="RHEA-COMP:16109"/>
            <dbReference type="Rhea" id="RHEA-COMP:16110"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:62525"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029986937.1">
        <property type="nucleotide sequence ID" value="XM_030131077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZQH1"/>
    <dbReference type="Ensembl" id="ENSSORT00005019989.1">
        <property type="protein sequence ID" value="ENSSORP00005019431.1"/>
        <property type="gene ID" value="ENSSORG00005009553.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417095"/>
    <dbReference type="CTD" id="196074"/>
    <dbReference type="InParanoid" id="A0A672ZQH1"/>
    <dbReference type="OrthoDB" id="16290at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.170:FF:000002">
        <property type="entry name" value="Probable methyltransferase-like protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00006">
        <property type="entry name" value="16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265:SF0">
        <property type="entry name" value="12S RRNA N4-METHYLCYTIDINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="S-ADENOSYL-METHYLTRANSFERASE MRAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="389" mass="43286" checksum="A55372FFE6A1BC0C" modified="2020-06-17" version="1">MIFQMSMRARSLLWKTNLSTWRICMTWKSSALHSTVNASSGLGPPPGCEEQSSVSIIPPHTPVMLKEVLHYLDVQPGQVVLDMTFGGGGHTRAILNMVPEVTVLAVDRDPTAISLAEHLAKEYSGRVKPMLGRFSDLEALLSDMKIKPGSVDAVLMDAGCSSMQMDQAQRGFSLSKDGPLDMRMDGERYPDMPCAADVVNTLDQQALASILTAYGEERHARKIASAIVEARRVSPITRTQQLASVVAGSFPAAVIYARKDRLHRPAHVATKTFQALRIFVNDELNELHAGLQAAQKIVRPGGRLCVITFHSLEDRLVKRFLQGEDLSNLDQYHFSPRKQGTRKEKLEEKRKNRSVFWLPVQKKVITPETDDVKENPRGRSAKLRVALRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZS90</accession>
    <name>A0A672ZS90_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein FAM111A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115432450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030009155.1">
        <property type="nucleotide sequence ID" value="XM_030153295.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030009156.1">
        <property type="nucleotide sequence ID" value="XM_030153296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZS90"/>
    <dbReference type="Ensembl" id="ENSSORT00005020732.1">
        <property type="protein sequence ID" value="ENSSORP00005020165.1"/>
        <property type="gene ID" value="ENSSORG00005009831.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432450"/>
    <dbReference type="InParanoid" id="A0A672ZS90"/>
    <dbReference type="OrthoDB" id="10025068at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14389:SF3">
        <property type="entry name" value="PROTEIN FAM111A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14389">
        <property type="entry name" value="SI:CH1073-475A24.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="681" mass="76367" checksum="2552370375251A8F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVV3</accession>
    <name>A0A672ZVV3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Paralemmin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Paralemmin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="20">Interacts with dopamine receptor DRD3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Apicolateral cell membrane</location>
            <topology evidence="18">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Basolateral cell membrane</location>
            <topology evidence="19">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Filopodium membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the paralemmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZVV3"/>
    <dbReference type="Ensembl" id="ENSSORT00005021868.1">
        <property type="protein sequence ID" value="ENSSORP00005021231.1"/>
        <property type="gene ID" value="ENSSORG00005010366.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZVV3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016327">
        <property type="term" value="C:apicolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498:SF6">
        <property type="entry name" value="PARALEMMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498">
        <property type="entry name" value="PARALEMMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="12 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0636">Prenylation</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="40"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="200"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="5"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00040790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="258" mass="27882" checksum="0C7D8A7F040A1463" modified="2020-06-17" version="1">MPQEKRKWQTEIENKKRQLEDDRRALQHLKSKALRERWLLDGAPSAGPEQDQVKTQLQQDEARTRTLEDTISRSVPEGFQTGLWASTNKQQKKSKVYENEQKGDSFVHEMNGDDGVQMLSSSEVEDSSTNHGDIPPHGDAAGGGGGAGWSPNRAASPSIAEASAENPVTMVFMGYQSVEDEDETKKVLGLRGTVKAELVVIDDGNGGDDGKTSPGEKSSPGGEAGPGRVQTEVAESESEGGAVQIKKEKQPCKCCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWI0</accession>
    <name>A0A672ZWI0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HECT domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hectd2</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZWI0"/>
    <dbReference type="Ensembl" id="ENSSORT00005021417.1">
        <property type="protein sequence ID" value="ENSSORP00005020792.1"/>
        <property type="gene ID" value="ENSSORG00005010148.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZWI0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="395" mass="45870" checksum="6873586ED7175BF7" modified="2020-06-17" version="1">YWTNTMKKNMTENRQKYRHNEALELPPICSDVRQKQRISIDTLPPEVKAPFPSDPVIPLRTKTTREFQEDMERAGQSGDWREVREFYLTTFDSFIEINAAFKREANGSFNTIDDSGVNAKFVNAVYDALLNTPQDIQKSVLKGIINSLLREWKGPRSKDDLRAYFILVQNPQYSSISTYVIYAHLLRQISVLSEADHHFLVHWLKRLSARRFRQLVERLLQFISTRLFPAEADELPPLAKCSWWIPSATKVLGLFNAANSVSSPPIMPFTDFYNITLDHIDFMEEYRTWQNYGNSNRFSFCQFPFILSTVVKKAIIQKDSEQQMISQARQSLVSKVSRRQRVDMNLLFLNIKVRRAQLLSDSLDEVSPNSNHCISHGGADEWSLLEKLIILKHVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWN0</accession>
    <name>A0A672ZWN0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gnb1b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZWN0"/>
    <dbReference type="Ensembl" id="ENSSORT00005022044.1">
        <property type="protein sequence ID" value="ENSSORP00005021399.1"/>
        <property type="gene ID" value="ENSSORG00005010435.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZWN0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="222"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="363" mass="40140" checksum="ADC0201A49C7022A" modified="2020-06-17" version="1">MSELDQLRQEAEQLKNQIRDARKACADATLSQITANIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFAGHTGDVMSLSLAPDSRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMIYSHDNIICGITSVAFSKSGRLLLAGYDDFNCNVWDTLKADRAGVLAGHDNRVSCLGVTDDDLELKPEASSSTLFKLTWTPQKRRETNAFPTQRRAQLFIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZYU0</accession>
    <name>A0A672ZYU0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutamate receptor interacting protein 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">grip2b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZYU0"/>
    <dbReference type="Ensembl" id="ENSSORT00005022308.1">
        <property type="protein sequence ID" value="ENSSORP00005021662.1"/>
        <property type="gene ID" value="ENSSORG00005007986.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZYU0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098887">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PDZ1_GRIP1-2-like"/>
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    </dbReference>
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        <property type="entry name" value="PDZ2_GRIP1-2-like"/>
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    </dbReference>
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        <property type="entry name" value="PDZ4_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ5_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ6_GRIP1-2-like"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
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    </dbReference>
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        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="GLUTAMATE RECEPTOR-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="918"/>
            <end position="1000"/>
        </location>
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        <location>
            <begin position="369"/>
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        </location>
    </feature>
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        <location>
            <begin position="724"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
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        <location>
            <begin position="733"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1019" mass="111228" checksum="16B5B62502882EFF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZ98</accession>
    <name>A0A672ZZ98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ADAM10 endopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.81</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">si:ch1073-396h14.1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase of broad specificity.</text>
            <dbReference type="EC" id="3.4.24.81"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.81" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZZ98"/>
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        <property type="protein sequence ID" value="ENSSORP00005022386.1"/>
        <property type="gene ID" value="ENSSORG00005010898.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:Notch signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
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    </dbReference>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
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        <property type="entry name" value="Disintegrin_dom"/>
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        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF1">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 10 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21299">
        <property type="entry name" value="ADAM10_Cys-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13574">
        <property type="entry name" value="Reprolysin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025403996" description="ADAM10 endopeptidase" evidence="7">
        <location>
            <begin position="16"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="648"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="9">
        <location>
            <begin position="206"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="8">
        <location>
            <begin position="434"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="681" mass="75842" checksum="388737661635CB29" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6V2</accession>
    <name>A0A673A6V2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">drd2a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A6V2"/>
    <dbReference type="FunCoup" id="A0A673A6V2">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026041.1">
        <property type="protein sequence ID" value="ENSSORP00005025285.1"/>
        <property type="gene ID" value="ENSSORG00005012163.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A6V2"/>
    <dbReference type="OrthoDB" id="10034726at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001591">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gi/Go"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007195">
        <property type="term" value="P:adenylate cyclase-inhibiting dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051481">
        <property type="term" value="P:negative regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051967">
        <property type="term" value="P:negative regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060158">
        <property type="term" value="P:phospholipase C-activating dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014059">
        <property type="term" value="P:regulation of dopamine secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043266">
        <property type="term" value="P:regulation of potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="D(2) dopamine receptor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000086">
        <property type="entry name" value="Dopamine D2 receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001922">
        <property type="entry name" value="Dopamine_D2_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF87">
        <property type="entry name" value="D(2) DOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00567">
        <property type="entry name" value="DOPAMINED2R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="69"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="308"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="488" mass="54879" checksum="56ED3FFEC0DA994F" modified="2020-06-17" version="1">MIYILFLKAGPLLGLCHSQLMDVFTQNAYNDSFFDNGTWSFNETDQEQKHPYNYYAMLLTLLIFVIVFGNVLVCMAVSREKALQTTTNYLIVSLAVADLLVATLVMPWVVYLEVVGEWRFSKIHCDIFVTLDVMMCTASILNLCAISIDRYTAVAMPMLYNTRYSSRRRVTVMISVVWVLSFAISCPLLFGLNNTATRDESLCVIANPAFVVYSSIVSFYVPFIITLLVYVQIYVVLRKRRKRVNTKPKQRVCQAAEPDVATSIKDKCTHPEDVRLCTMIVKSNGSFPVNKKKVIFIKEVVNEGEDLELDDLNNSGSSQKQKQQPQCALGDTPATSNQQLMPNKANDSPTATPPTPPDEGQKTEKNGDPTKEALGDPAPVVVKAFQTQALPNGKTQTSVKTMSKRKISQQKEKKATQMLAIVLGVFIICWLPFFITHILNTHCTKCKVPAEMYNAFTWLGYVNSAVNPIIYTTFNVEFRKAFIKILHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8T9</accession>
    <name>A0A673A8T9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lingo4b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A8T9"/>
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        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026387.1">
        <property type="protein sequence ID" value="ENSSORP00005025624.1"/>
        <property type="gene ID" value="ENSSORG00005012319.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A8T9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025353989" evidence="12">
        <location>
            <begin position="26"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="540"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="401"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="604" mass="67356" checksum="0D7752BEE095CBBC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAB3</accession>
    <name>A0A673AAB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673AAB3"/>
    <dbReference type="Ensembl" id="ENSSORT00005026983.1">
        <property type="protein sequence ID" value="ENSSORP00005026211.1"/>
        <property type="gene ID" value="ENSSORG00005012580.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AAB3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000660">
        <property type="term" value="P:negative regulation of interleukin-1-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034122">
        <property type="term" value="P:negative regulation of toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903093">
        <property type="term" value="P:regulation of protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF9">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="7">
        <location>
            <begin position="28"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="6">
        <location>
            <begin position="254"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="34103" checksum="319CE2F1700D3336" modified="2020-06-17" version="1">MDGGGSMRSNEERVAEKQMDDYLKTIGLHRKKIAKDGSCLFRAVAEQVLYCQSYHTEVRAQCVEFLKKNRDSYEAFIEGDFEDYLCKLQDPQQWVGEVEINALAVMYKRDFLIYQEPNKPAVHITDNNFKDKVRLCFLNGNHYDSVYPISHMKGAAVCQSILYELLYTNVFKVDRSVLAGYQRTGRHFEVLSDDSMAACISSDESDGRGRGRHLSDRVRRSLNPTLFRNIEFDVWQRSKKAQQKLDFCIAAGMQYAVGDRCQVRLEGRTYNATIKEVPPNNSAVTVYVEELGRRWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AC16</accession>
    <name>A0A673AC16_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Bone morphogenetic protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">bmp10</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AC16"/>
    <dbReference type="FunCoup" id="A0A673AC16">
        <property type="interactions" value="1769"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027915.1">
        <property type="protein sequence ID" value="ENSSORP00005027130.1"/>
        <property type="gene ID" value="ENSSORG00005012971.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AC16"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035239">
        <property type="term" value="P:tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF39">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025410627" evidence="10">
        <location>
            <begin position="32"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="11">
        <location>
            <begin position="377"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="489" mass="55344" checksum="3878B1EEED5D540B" modified="2020-06-17" version="1">TSLHFNFFWTCVTWLLLVFIPLLQGPLCVESSPISNTHQRYRPAPGLGDGHGGVVDPSMLEQDSNMDMQSLLESLKEQFLRTFNLSGMGPPPLPDGSTREEPPEYMVELYNRFANDRTAMPTANIIRSFKNEDSSPSVVGAGGVRRHLLLFNVSIPHHERVTAAELRLYTLVQTDRHLYAGVDRKVTIYELESHDGDDNMTDENPMRGDTFREETERIELVELASRQVYGTDNGWEAFDLTAAVQRWRKSDHGTTHQLEVHIASVASEDPTQNVAQEHEDNNPTEGDMKIDISPEEKHKPLLIVFSDDQSSDHRDDKRELNEMIDHETSNMVLQNDLTGLNGLWGDMDKDKDEEEAEPDEEDLIQMRSNLIYDTASRIRRNAKGNHCKKQSLYVEFKDIGWDSWILAPTGYDAFECTGICSFPLTKHVTPTKHAIVQTLININSPQKAARACCVPTKLDPISLLYLDDTGVVTYKYKFEGMVVAECGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEW4</accession>
    <name>A0A673AEW4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Reticulon 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AEW4"/>
    <dbReference type="Ensembl" id="ENSSORT00005027726.1">
        <property type="protein sequence ID" value="ENSSORP00005026942.1"/>
        <property type="gene ID" value="ENSSORG00005012899.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AEW4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071787">
        <property type="term" value="P:endoplasmic reticulum tubular network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF7">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="7">
        <location>
            <begin position="12"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="203" mass="23107" checksum="DB2414CD50968028" modified="2020-06-17" version="1">KHTKVMLLDKYNMRKTGFVFTGLVVGLASLFQLSAITVLSHVCLGIMCVTFPLRLYYKLLELLRWNPGVHPFQTYLDYDNSLTDKETVVLVEEVVLLIAFAVTEIKRLLFIENVIDSIKFVVLLYLLSYVGVLTTGLTLVIAVVIAVFSLPLLYKKQQVRFFCSMKSCLSPIFNSFVACYYLCDLFLSFLLCVKLVAANKKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AF17</accession>
    <name>A0A673AF17_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115410736</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AF17"/>
    <dbReference type="Ensembl" id="ENSSORT00005028686.1">
        <property type="protein sequence ID" value="ENSSORP00005027889.1"/>
        <property type="gene ID" value="ENSSORG00005013353.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AF17"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025553575" description="Fibrinogen C-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="26492" checksum="3A1C2230D64A5C43" modified="2020-06-17" version="1">LLLLLLLSLLLCFQSITNFRDITMKYLRPGGTDRRVWFPNSCVCVGLLRHGDGRRRMDGERMDGSVGFHRPWDQYKKGFGDVNGEYWLGLDNIHYLTRRRDYELLVEMSDFDGNNVSARYATFFVGGEHRGYELHISEFTDGGAGDALSSHNGMKFSTFDKDQDPSSYNCAKTYLGGFWHHAYCAYGNPNGAYLWGYDGNYKFGVFWYYWKGYYYSMKNFSMKIRPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AFX0</accession>
    <name>A0A673AFX0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">stat1a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AFX0"/>
    <dbReference type="Ensembl" id="ENSSORT00005028957.1">
        <property type="protein sequence ID" value="ENSSORP00005028154.1"/>
        <property type="gene ID" value="ENSSORG00005013468.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AFX0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051093">
        <property type="term" value="P:negative regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060337">
        <property type="term" value="P:type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060333">
        <property type="term" value="P:type II interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000012">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.532.10:FF:000001">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.20:FF:000001">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000001">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.505.10:FF:000003">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="6.10.250.3310:FF:000002">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3310">
        <property type="entry name" value="signal transducer and activator of transcription 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
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    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
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    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
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        <property type="entry name" value="STAT1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022752">
        <property type="entry name" value="STAT1_TAZ2-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
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        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12162">
        <property type="entry name" value="STAT1_TAZ2bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48092">
        <property type="entry name" value="Transcription factor STAT-4 N-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15 19" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="17 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 19" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18 19" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="16 19" id="KW-0804">Transcription</keyword>
    <keyword evidence="12 19" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SH2" evidence="20">
        <location>
            <begin position="571"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="763" mass="88346" checksum="3F4AF1498CC6F084" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AK58</accession>
    <name>A0A673AK58_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115410020</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029977285.1">
        <property type="nucleotide sequence ID" value="XM_030121425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AK58"/>
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        <property type="interactions" value="1306"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030587.1">
        <property type="protein sequence ID" value="ENSSORP00005029751.1"/>
        <property type="gene ID" value="ENSSORG00005014218.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410020"/>
    <dbReference type="InParanoid" id="A0A673AK58"/>
    <dbReference type="OrthoDB" id="10070184at2759"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="H1/H5"/>
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        <property type="entry name" value="Histone_H1/H5_H15"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="6">
        <location>
            <begin position="26"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="81"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="105"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="198" mass="20115" checksum="5ACFD7D912A3A4F1" modified="2020-06-17" version="1">MAEVAPAPAAAPAKAAKKKAVKKAKTGPSVRDLITKTVAASKERNGVSLAALKKALAAAGYDVEKNKGRIKVAVKSMVASGVLTQTKGTGASGSFKMSKKAEAKPKKKAVKKAAPKAKKAAAKKPATPKRPKKPAAAKKAPKKSPKKAAKKAPAAKKAAAKSPKKAPKKVTKKPAAKKAPAKKAAAKPKAKKAAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKQ0</accession>
    <name>A0A673AKQ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-CoA thioesterase 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115429083</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AKQ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005031115.1">
        <property type="protein sequence ID" value="ENSSORP00005030270.1"/>
        <property type="gene ID" value="ENSSORG00005014428.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AKQ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2240:FF:000002">
        <property type="entry name" value="Acyl-CoA thioesterase 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000024">
        <property type="entry name" value="acyl-coenzyme A thioesterase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2240">
        <property type="entry name" value="Acyl-CoA thioester hydrolase/BAAT N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016662">
        <property type="entry name" value="Acyl-CoA_thioEstase_long-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014940">
        <property type="entry name" value="BAAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006862">
        <property type="entry name" value="Thio_Ohase/aa_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042490">
        <property type="entry name" value="Thio_Ohase/BAAT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824:SF36">
        <property type="entry name" value="ACYL-COA THIOESTERASE 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08840">
        <property type="entry name" value="BAAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04775">
        <property type="entry name" value="Bile_Hydr_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016521">
        <property type="entry name" value="Acyl-CoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase" evidence="3">
        <location>
            <begin position="20"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="BAAT/Acyl-CoA thioester hydrolase C-terminal" evidence="4">
        <location>
            <begin position="212"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR016521-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="426" mass="47521" checksum="F95EA258D377539B" modified="2020-06-17" version="1">TREQAYPPILSVYPSRALVDEAFKVLVENLPPGCPVTVHSLHLSEDDNYWEAYGHYISDHRGSVSVSEDVSFGGTYTGKEPMGLLWSLRPVPGSRQGLRLRKMNVCTPMLVNISVHCGHLVEAFRDQTPLASVLTERWYMAPGVKRIDIRERGMRGTLFIPPGPGPFPGLLDLWGGGGGLLEHRAALLASHGFVSLALEYLTSGEIPTARLDYNYFEKAFNIIQDHPLVTPDRVGIFGLSLGSFVTFNLAAKSTIINPRCCVCISGFHCHPYRSMGEMIQTNLNKLHVDENNYQIWRDMSLPIPSDISKKVDMGKIKCPLLLVCGHDDQSYPAVELAEDIAQMVHAAGKDHLLTKVFYHGTGHMIEPPYSPHFRATTFMAKTKPKVILLWGGQTKPHSDAQEDSWKKILAFLQHHLYSTPNPRAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKY7</accession>
    <name>A0A673AKY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIE subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gtf2e2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TFIIE beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004014.1">
        <property type="nucleotide sequence ID" value="XM_030148154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AKY7"/>
    <dbReference type="Ensembl" id="ENSSORT00005030863.1">
        <property type="protein sequence ID" value="ENSSORP00005030021.1"/>
        <property type="gene ID" value="ENSSORG00005014339.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428904"/>
    <dbReference type="CTD" id="2961"/>
    <dbReference type="InParanoid" id="A0A673AKY7"/>
    <dbReference type="OrthoDB" id="5323195at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07977">
        <property type="entry name" value="TFIIE_beta_winged_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000177">
        <property type="entry name" value="Transcription initiation factor IIE subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003166">
        <property type="entry name" value="TFIIE_bsu_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02186">
        <property type="entry name" value="TFIIE_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016398">
        <property type="entry name" value="TFIIE-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51351">
        <property type="entry name" value="TFIIE_BETA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIE beta" evidence="9">
        <location>
            <begin position="55"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="11"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="241"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR016398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="287" mass="32638" checksum="D47EC282A23F1D45" modified="2020-06-17" version="1">MDPALLRERELFKKRALSTPAVEKRPASSESSSHKKKKTKVDKESSSSSKHGAESSNGNINIKTSSGYKFGCLAKIVNYMKTRHQNGDTHFLTLEEILDETKLLDISMKQKQWLMTEALVNNPKIDVRDGTYGFKPKYNLKDKKALLRLLDKHDQLGLGGVLLDDVEEGLPNSAKAIKALGDQIIFVTRPDKKKILFYNDKHCQFAVDEEFQKLWRSVPVDSMDEEKIEEYLKKQGISSMQETGPKKVLQVQKRKKQGGQRKRHFKTHNNHLAGVLEDYSDGVPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AN51</accession>
    <name>A0A673AN51_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zebrafish testis-expressed 38</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AN51"/>
    <dbReference type="Ensembl" id="ENSSORT00005030675.1">
        <property type="protein sequence ID" value="ENSSORP00005029837.1"/>
        <property type="gene ID" value="ENSSORG00005014258.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AN51"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051294">
        <property type="entry name" value="HORMA_MeioticProgression"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225">
        <property type="entry name" value="HORMA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225:SF4">
        <property type="entry name" value="ZEBRAFISH TESTIS-EXPRESSED 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="7">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="184"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="220" mass="25338" checksum="3D6F7DAFE9D27AFE" modified="2020-06-17" version="1">MMAVAVSSITYLRGIFPEVAYRSRYLEDLCIKVLREDCSTPGANKVVKWLMGCFDALEKQYCVVESYQFKFKYTEKGPEMDVLRNNDVKMQVTLEDTKRASVLLIRKLFLLMQNLGSLPNNVYLTMKLYYYDDITPADYEPPGFKEGECDSLWFEGMAVHFRVGEVQTPFHTLKVNVSAEQGRLEKLQDGNHLRESKVHTEKPPKEDKAITVSPEVDDLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AN74</accession>
    <name>A0A673AN74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115412350</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AN74"/>
    <dbReference type="Ensembl" id="ENSSORT00005030890.1">
        <property type="protein sequence ID" value="ENSSORP00005030048.1"/>
        <property type="gene ID" value="ENSSORG00005014349.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AN74"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18729">
        <property type="entry name" value="PIN_Zc3h12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040546">
        <property type="entry name" value="Rege-1_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040757">
        <property type="entry name" value="Regnase_1/ZC3H12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF36">
        <property type="entry name" value="RIBONUCLEASE ZC3H12C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18561">
        <property type="entry name" value="Regnase_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18039">
        <property type="entry name" value="UBA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="341"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="341"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="439"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="539"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="446"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="726" mass="80108" checksum="0AFC54A3A560513E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AR73</accession>
    <name>A0A673AR73_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 125b</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AR73"/>
    <dbReference type="Ensembl" id="ENSSORT00005033047.1">
        <property type="protein sequence ID" value="ENSSORP00005032160.1"/>
        <property type="gene ID" value="ENSSORG00005015285.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AR73"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028165">
        <property type="entry name" value="TMEM125"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31416">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31416:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15109">
        <property type="entry name" value="TMEM125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="229" mass="24756" checksum="1690DEB0D2F6D95C" modified="2020-06-17" version="1">NGPGAKSGPPKGSLDPALIHHRVLDDQVELWWSREPRRSLLCYCASVALILGLGLGGVGLLSTTTSLSGEWRLGVGTTLCLLSLAVLLKQLLSSAIQDMNCVRSRRRIDQLKSGGRADPALILAVGLAVTLCGTVLLCMVTVGSQGQDRGDMLVSGLVLMAAGTGMTLAVVSYSTLVYLKRRREQRRRQRMRMSRARRLGSRAVRVFNVSGREISQANREISTSRTSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARC0</accession>
    <name>A0A673ARC0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kazrin, periplakin interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ARC0"/>
    <dbReference type="Ensembl" id="ENSSORT00005032931.1">
        <property type="protein sequence ID" value="ENSSORP00005032046.1"/>
        <property type="gene ID" value="ENSSORG00005015226.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARC0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037614">
        <property type="entry name" value="Kazrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059089">
        <property type="entry name" value="Kazrin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776:SF3">
        <property type="entry name" value="KAZRIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776">
        <property type="entry name" value="KAZRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25986">
        <property type="entry name" value="Kazrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="343"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="429"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="658" mass="74804" checksum="CC721A21BE9D161F" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AY00</accession>
    <name>A0A673AY00_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">hexokinase</fullName>
            <ecNumber evidence="6">2.7.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115418183</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>D-glucose + ATP = D-glucose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:17826"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673AY00"/>
    <dbReference type="Ensembl" id="ENSSORT00005035440.1">
        <property type="protein sequence ID" value="ENSSORP00005034520.1"/>
        <property type="gene ID" value="ENSSORG00005010248.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AY00"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000015">
        <property type="entry name" value="Hexokinase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000001">
        <property type="entry name" value="Hexokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000020">
        <property type="entry name" value="Hexokinase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF10">
        <property type="entry name" value="HEXOKINASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025629850" description="hexokinase" evidence="18">
        <location>
            <begin position="20"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase N-terminal" evidence="19">
        <location>
            <begin position="25"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="20">
        <location>
            <begin position="233"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase N-terminal" evidence="19">
        <location>
            <begin position="477"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="20">
        <location>
            <begin position="673"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="919" mass="103180" checksum="104D454544F994AB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AY77</accession>
    <name>A0A673AY77_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-6 superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AY77"/>
    <dbReference type="FunCoup" id="A0A673AY77">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034160.1">
        <property type="protein sequence ID" value="ENSSORP00005033253.1"/>
        <property type="gene ID" value="ENSSORG00005015758.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AY77"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005127">
        <property type="term" value="F:ciliary neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070120">
        <property type="term" value="P:ciliary neurotrophic factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000151">
        <property type="entry name" value="Ciliary_neurotrophic_fac_CNTF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010681">
        <property type="entry name" value="PRF/CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21353">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21353:SF9">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01110">
        <property type="entry name" value="CNTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025407374" evidence="5">
        <location>
            <begin position="35"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="221" mass="25936" checksum="AD0A378E50F509EC" modified="2020-06-17" version="1">FSPEFTHHASSPLHMTIWWQALILLCYILNCVDSSAPSLVLQTKYKNSFRLTRSTRTRVQQLLLNYKKQQLGNKDFEDRSQHLKDLPVLSTDFYNWLNLSDWERLYAAVGDMQVYWNMLEWKRKQLEKADKEQMVAQTTLPQSIRHIQLDLRDLMSQVNEYMKSTSDSPTSTTVQTSMNPGSNSKTIWDKRVEGYIILRDLDLYLTKLARDFLLLASKAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZT6</accession>
    <name>A0A673AZT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mafaa</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AZT6"/>
    <dbReference type="FunCoup" id="A0A673AZT6">
        <property type="interactions" value="85"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005035175.1"/>
        <property type="gene ID" value="ENSSORG00005016594.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5974330at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAF bZIP transcription factor"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bZIP"/>
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        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
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        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
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        <property type="entry name" value="Maf_TF_N"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="233"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
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        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="321" mass="35070" checksum="C9961953200B615F" modified="2020-06-17" version="1">MASDLGMSAELPNSPLAIEYVNDFDLMKFEVKKEPPEADRYCHRLPSGSLSSTPISTPCSSVPSSPSFCAPSPGAQPNQSLGGGGGGGGGGVSGGGGGGSGSGGNGHGGGGGAGKPQLEDLYWIPSYQHHINPEALNLTPEDAVEALIGNAHHHHHHHQAYEGFRGQQYVGEDLSAASAAAHHHPAHHHHHHHHHHGHHGRLEDRFSDEQLVSMTVRELNRQLRGFSKEEVIRLKQKRRTLKNRGYAQSCRFKRVQQRHMLETEKCTLQNQVEQLKQDVARLAKERDLYKEKYEKLASRTFSGPGSARDPNGKQANPEFFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B189</accession>
    <name>A0A673B189_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Adhesion G protein-coupled receptor E8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B189"/>
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        <property type="protein sequence ID" value="ENSSORP00005034303.1"/>
        <property type="gene ID" value="ENSSORG00005016221.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_2_EMR1-like_rcpt"/>
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    </dbReference>
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        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF469">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EMR1HORMONER"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="231"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="314"/>
        </location>
    </feature>
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        <location>
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            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="8">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
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            <begin position="138"/>
            <end position="385"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="47218" checksum="276220A6C9E2E993" modified="2020-06-17" version="1">SSLTANGNTMEINMGTLAENNNAAAFLTLHGMESLLSHQYFKTENRTEMYSDVITAVLPSVNNTNLTEPVNFTVQHKKKLPGDGMVTCVYWEGKPEEAQGGKKTGGKTSTMRWSVDGCWVAYTTDNYTVCSCSHLSTFALIMQIGEWLNRMCVIVGFFFFALAILTFLLCSWNPKINNTARLHLCLSLSLSHLLLLWNEKYLACTVMAGILHFLVVASFVWMQLEAVQLYLLVRRLTKVQVIQRDGLPRPLLYVIGYGVPFVIVGVSALVYSDGYGATEAGACWLSQKRNFNWALTGPVITILLNCMVFCATLWSLRTTLANMKSGVSQSTDTRLIVFKIVAQFFILGCTWILGLYQTNVFFRVLFIILNSQQGTFLFIVHCLLNKEVDSLHPFNLFLFRSCPCFFKMHRITSEFYLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5N8</accession>
    <name>A0A673B5N8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC115437363</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B5N8"/>
    <dbReference type="Ensembl" id="ENSSORT00005038103.1">
        <property type="protein sequence ID" value="ENSSORP00005037130.1"/>
        <property type="gene ID" value="ENSSORG00005017444.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5N8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000001">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041485662" description="Zona pellucida sperm-binding protein 3" evidence="14">
        <location>
            <begin position="25"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="16">
        <location>
            <begin position="72"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="358"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="450" mass="50662" checksum="4B89A6B16FD1B15B" modified="2020-06-17" version="1">MDHDLQRAFTWWMLLFFSLHTVSQSRFSYKPGAAHSPIQSRTHGGGLHPPVSTGKHPQPTQAPARPRPVLVKCHPDSMEVVVQADVFDTGLLVELTHLRLGSEAHGEGACRPVPSGHAEYTIWASLTDCGTKLSSTKEKIIYSNVLVYSPEPSSNGLLRLDGATIPVECHYERVYSVEGISLLPTWVPSVSRVTAEDKMDFSLRIMTADWQFERGSHTYFLGDPIHFEVSSIMGNHRPLCVFVDRCVATATPDTEATLRYNFIEPDGCLADAYLTNSSSRFLPRMEEHKLRFQIDAFKFYKNPSNQVYITCYMSAVPVVVNITSQKRACSFIENRWLSADGNNQDCRSCDLTYQVKKPPSTEPPKTTISIKPRPPVASKENLIPSRAEPPASFVRVRTGMQHYPHSSLQKWGKKKILKIHKKNKNNFDVLFPKCYEIYSGSLAIYNPNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B724</accession>
    <name>A0A673B724_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">med8</name>
        <name evidence="8" type="synonym">MED8</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987573.1">
        <property type="nucleotide sequence ID" value="XM_030131713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B724"/>
    <dbReference type="FunCoup" id="A0A673B724">
        <property type="interactions" value="1388"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037385.1">
        <property type="protein sequence ID" value="ENSSORP00005036422.1"/>
        <property type="gene ID" value="ENSSORG00005017170.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417692"/>
    <dbReference type="CTD" id="112950"/>
    <dbReference type="InParanoid" id="A0A673B724"/>
    <dbReference type="OrthoDB" id="150687at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019364">
        <property type="entry name" value="Mediatior_Med8_fun/met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074:SF9">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10232">
        <property type="entry name" value="Med8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="224"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="270" mass="29424" checksum="E0CA0EC8FCE15BB3" modified="2020-06-17" version="1">MQQREEKQLEASVESLISRVAHVKNALHGFIYKLENEYERLTWPSVLDNFALLSGQLNTINKLLKNEKTPSFRSQVIIPLLLNPDRDEDLAKLTEQRVPVFSHEIVPDYLRTKPDPEVEEQEKQLSAEAARIGPEVAQKQIQTLNKLCSNLLEKLNNPRDDRDAESAALRQNKPSFNPADTNALVGAVAFGKGLSKCRPPGPVAPGHPGQGPMMSGGPTLQQVTIGGGSGQQAGMGGPVAPQQQGQPGKMPSSIKTNIKSASGSMHPYNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9B5</accession>
    <name>A0A673B9B5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Suppressor of IKBKE 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Suppressor of IKK-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sike1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIKE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029995609.1">
        <property type="nucleotide sequence ID" value="XM_030139749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B9B5"/>
    <dbReference type="FunCoup" id="A0A673B9B5">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038891.1">
        <property type="protein sequence ID" value="ENSSORP00005037904.1"/>
        <property type="gene ID" value="ENSSORG00005017777.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423047"/>
    <dbReference type="CTD" id="80143"/>
    <dbReference type="InParanoid" id="A0A673B9B5"/>
    <dbReference type="OrthoDB" id="21214at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008555">
        <property type="entry name" value="SIKE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12186">
        <property type="entry name" value="SIKE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12186:SF4">
        <property type="entry name" value="SUPPRESSOR OF IKBKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05769">
        <property type="entry name" value="SIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="25856" checksum="90FAEBA1F1211AE8" modified="2020-06-17" version="1">MACTLEKVLGDARTLLDRLKEHDTAAEGLIEQSGALSQRVQSMREVGNALPDKHIEDTSVIQELTKCKPHILLTQENTQIKDLQQENKELWLSLEEHQYALELIMGRYRKQMLQLMMAKKELDTKPVLSLHEDHAKEVQSQVERICEMGQVMRKAVQVDDQHYCSVRERLAQLEIENKELRDLLVISRGSVKMMREDGNQPATTATSAAAATSAAEQEQTPQPPESTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB36</accession>
    <name>A0A673BB36_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BB36"/>
    <dbReference type="Ensembl" id="ENSSORT00005040042.1">
        <property type="protein sequence ID" value="ENSSORP00005039040.1"/>
        <property type="gene ID" value="ENSSORG00005018246.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BB36"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF102">
        <property type="entry name" value="CEREBELLIN 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025422566" description="C1q domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="5">
        <location>
            <begin position="182"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="318" mass="36976" checksum="5E02FACFA9FF3F57" modified="2020-06-17" version="1">MRFHRVNVVFLVFLLCLNWTWAQEKNGGYDESKFAEETDERSEINDNRQKNISPDIWTELKELRDMVIKQEMPLGFYHMDVLTVLETRLSCSQTEVEELKRENAGKYLLKHKYELRSLKYLFRQSPRLIDISNTCVWLYMVNVSFSTDLRDRITASENKSTVLESRLSSSETEVEELKRENAERPKVAFSFALTDAGRIGPFNTDITLKFTKVFTNFGQAYNPNTGIFIAPTRGVYYFRFSAMNNNRNAWLGVKLYLNDKKIALNDDIKSVGNIRVTNAYVLQLEKGDVVYLVLRTGHAIFDDYNNHTTFSGFLLFPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BBQ4</accession>
    <name>A0A673BBQ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BBQ4"/>
    <dbReference type="Ensembl" id="ENSSORT00005039704.1">
        <property type="protein sequence ID" value="ENSSORP00005038709.1"/>
        <property type="gene ID" value="ENSSORG00005018084.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BBQ4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="76" mass="8533" checksum="2C1A25B182C26BD4" modified="2020-06-17" version="1">LHLQRETCGPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYVDGALAGHLGEVLIRYVVNINLWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BET3</accession>
    <name>A0A673BET3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DEAH (Asp-Glu-Ala-His) box polypeptide 34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dhx34</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030007565.1">
        <property type="nucleotide sequence ID" value="XM_030151705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BET3"/>
    <dbReference type="Ensembl" id="ENSSORT00005040833.1">
        <property type="protein sequence ID" value="ENSSORP00005039799.1"/>
        <property type="gene ID" value="ENSSORG00005018572.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431389"/>
    <dbReference type="CTD" id="9704"/>
    <dbReference type="InParanoid" id="A0A673BET3"/>
    <dbReference type="OrthoDB" id="3363059at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17979">
        <property type="entry name" value="DEXHc_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000013">
        <property type="entry name" value="ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000540">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000725">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056382">
        <property type="entry name" value="DHX34_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24485">
        <property type="entry name" value="zf-C2H2_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="168"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="364"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="715"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1087"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="715"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1087"/>
            <end position="1107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1148" mass="130460" checksum="2AD8F0B8323834FB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BH14</accession>
    <name>A0A673BH14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lpgat1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984323.1">
        <property type="nucleotide sequence ID" value="XM_030128463.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029984324.1">
        <property type="nucleotide sequence ID" value="XM_030128464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BH14"/>
    <dbReference type="Ensembl" id="ENSSORT00005041610.1">
        <property type="protein sequence ID" value="ENSSORP00005040559.1"/>
        <property type="gene ID" value="ENSSORG00005018936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415048"/>
    <dbReference type="CTD" id="9926"/>
    <dbReference type="InParanoid" id="A0A673BH14"/>
    <dbReference type="OrthoDB" id="5920068at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF2">
        <property type="entry name" value="ACYL-COA:LYSOPHOSPHATIDYLGLYCEROL ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="95"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="372" mass="43207" checksum="C0C4B58810861A97" modified="2020-06-17" version="1">MAQLLDGAGKLGWVLLKSVLRFVFMFFNNCVAIPSYCLYLLLLQPLRIWDTSTFWHIEGVMFKWLLAMVSSWGWIAGYTVTEWGDDVRPISEEEAMVIVNHQSTGDVCTLMMCLQDKGTVVRKMMWLMDHVFKYTNFGLVSLIHGDFFIRQGKAHRDKQLVYLKDHLDKYYHSRDRKWIVLFPEGGFLRKRRETSQLFAKKHSLPHLTHVTLPRLGATHIILKTLSAQQENGSVGGEAGTLNQTGIKRKGLQWVVDVTIAYPKARPMDIQTWIFGYRPPTVTHVHYRMYPIKEVPVEAEALTDWLYQRFVEKEELLAHFYDTGAFPPLPGQKEAVSRQMTLDPVWLCVIQSFAFVSGYMWYSVLQYLYCCLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIW2</accession>
    <name>A0A673BIW2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-microseminoprotein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BIW2"/>
    <dbReference type="Ensembl" id="ENSSORT00005043590.1">
        <property type="protein sequence ID" value="ENSSORP00005042511.1"/>
        <property type="gene ID" value="ENSSORG00005019697.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BIW2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1900">
        <property type="entry name" value="Beta-microseminoprotein (PSP94) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008735">
        <property type="entry name" value="PSP94"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10500">
        <property type="entry name" value="BETA-MICROSEMINOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10500:SF4">
        <property type="entry name" value="PROSTATE-ASSOCIATED MICROSEMINOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025389870" evidence="5">
        <location>
            <begin position="19"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="159" mass="17713" checksum="802C2B2FF9CBB79F" modified="2020-06-17" version="1">MFFALFIFLFNLYLPRQAIKNRFLFPNAGKGWGWGCFLLSALYTKPSHLHVASVFAAAQCVFEGRHYSLGEAWMDNACMQCTLNKHPVVHRPVDFPAWCEVRVEPVTCKVSLVQTADPRLPCVPGETNRDPSHGSLFTPVGPSPLTVHRSTTPTGLVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLC3</accession>
    <name>A0A673BLC3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein S100-P-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115438909</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030018659.1">
        <property type="nucleotide sequence ID" value="XM_030162799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BLC3"/>
    <dbReference type="Ensembl" id="ENSSORT00005042445.1">
        <property type="protein sequence ID" value="ENSSORP00005041382.1"/>
        <property type="gene ID" value="ENSSORG00005019254.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438909"/>
    <dbReference type="InParanoid" id="A0A673BLC3"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00213">
        <property type="entry name" value="S-100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000044">
        <property type="entry name" value="Protein S100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034325">
        <property type="entry name" value="S-100_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF134">
        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="92" mass="10012" checksum="FBD9BABFF5C40DB8" modified="2020-06-17" version="1">MTQLETAMAILMKTFDTYAGAEGSKESLSKAEVKTLLEKELPGLLKAAKNQEEVDKLLKALDMNGDSEVDFSEFVVLVAALTCACHDRCGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLX2</accession>
    <name>A0A673BLX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentraxin family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115435987</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the pentraxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BLX2"/>
    <dbReference type="Ensembl" id="ENSSORT00005042649.1">
        <property type="protein sequence ID" value="ENSSORP00005041582.1"/>
        <property type="gene ID" value="ENSSORG00005019333.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLX2"/>
    <dbReference type="OrthoDB" id="547680at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051005">
        <property type="entry name" value="Pentraxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869:SF7">
        <property type="entry name" value="C-REACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869">
        <property type="entry name" value="C-REACTIVE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025683649" description="Pentraxin family member" evidence="10">
        <location>
            <begin position="16"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="11">
        <location>
            <begin position="21"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="246" mass="28267" checksum="8B2BC7E57D2A6FBC" modified="2020-06-17" version="1">MKILVLLLMLTTCAADYQDLTGKMFTFPQEGNKAHVRLNTPKQNFKVVTVCHRSFTDLKRNHGLFSLATPNYFNGFLIYYNYSSREMEPYVRDSRSKYGGLDYKPNVWHSICTTWDSGSGLMQLWFNGQPLTKKYVSSGEISGRPIIILGQEQDSHGGKFDPTQSFVGMMTDVHMWDYALSACEIQKYMDEQNFTPGNVLNWAALDFQITDRVLIESKQMTCHLNICTMFSQTQLIRRFRSSLGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNP7</accession>
    <name>A0A673BNP7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Heterogeneous nuclear ribonucleoprotein A/B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC115433327</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APOBEC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030010534.1">
        <property type="nucleotide sequence ID" value="XM_030154674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BNP7"/>
    <dbReference type="Ensembl" id="ENSSORT00005044479.1">
        <property type="protein sequence ID" value="ENSSORP00005043385.1"/>
        <property type="gene ID" value="ENSSORG00005020035.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433327"/>
    <dbReference type="InParanoid" id="A0A673BNP7"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12757">
        <property type="entry name" value="RRM1_hnRNPAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000186">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein A/B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000030">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012956">
        <property type="entry name" value="CARG-binding_factor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034846">
        <property type="entry name" value="hnRNPAB_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF1">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08143">
        <property type="entry name" value="CBFNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="56"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="140"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="38"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="316" mass="35012" checksum="DE2DE2584F97B8A1" modified="2020-06-17" version="1">MADAENLLMETSEQNGNEGEEDLNGAEQELMAGDEEHDNGTDGGKIDASKGEEDAGKMFVGGLSWDTSKKDLKDYFTKFGEVSDCTIKMDSNTGRSRGFGFVLFKDSSSVDKVLEQKEHKLDGRQIDPKRAMAMKKEPVKKIFVGGLNPEATEDTIREYFGAFGEIETIELPMDPKTKKRRGFIFITYKEEASVKKCLEKKYHTIQGSRCELKIAQPKEVYQQQQFGGGRGGGYGGGRGGRGRGGQSQGWNQGYGNYWNQGYGNQGYGYGGYSGYGNYDYSSGYYGYGPGYDYDQGNASYGKTPRRGAHQTSYKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQ02</accession>
    <name>A0A673BQ02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alanine transaminase</fullName>
            <ecNumber evidence="8">2.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutamate pyruvate transaminase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glutamic--alanine transaminase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Glutamic--pyruvic transaminase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">gpt</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-alanine + 2-oxoglutarate = pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:19453"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673BQ02"/>
    <dbReference type="Ensembl" id="ENSSORT00005045953.1">
        <property type="protein sequence ID" value="ENSSORP00005044821.1"/>
        <property type="gene ID" value="ENSSORG00005020582.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQ02"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000226">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000345">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF308">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="15">
        <location>
            <begin position="186"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="25"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="559" mass="61262" checksum="53AC8EED0158A478" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQT2</accession>
    <name>A0A673BQT2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proteasome 26S subunit, non-ATPase 11a</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BQT2"/>
    <dbReference type="Ensembl" id="ENSSORT00005045260.1">
        <property type="protein sequence ID" value="ENSSORP00005044140.1"/>
        <property type="gene ID" value="ENSSORG00005020316.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQT2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="1">
        <location>
            <begin position="219"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="34896" checksum="B5A76FDEEC7213AD" modified="2020-06-17" version="1">RDSILELGGLLAKTGQAAELGGLLKYAARLVRSLLDLFLDHGGGHGAGGVSCVWSASSGPRPRKRTFLRQALEVTSFRLGSQLLQELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANASTAPPNCRQHWTCSQESSTAAEEKDWKTIMLMPEDVQALISGKLALRYAGRQTDSLKCVALASQNRSLADFEQALTEYKSELRDDPIISTHLTKLYHNLLEQNLIRVIEPFSRVQVGPSPPPESNVERKLSQMILDKKFTVGILDQGAGVLIVFEEPVVDQTYESALETIHNMSKVVDSLYNKAKKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BT48</accession>
    <name>A0A673BT48_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">WD repeat domain 90</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">wdr90</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Neoteleostei</taxon>
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            <dbReference type="Pfam" id="PF23409"/>
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    </evidence>
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</entry>
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    <accession>A0A673BWP9</accession>
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            <fullName evidence="22">Sodium/potassium-transporting ATPase subunit alpha</fullName>
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    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
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            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Cell membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BWP9"/>
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        <property type="protein sequence ID" value="ENSSORP00005045564.1"/>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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    <sequence length="2172" mass="247262" checksum="34AA9C9739634F8C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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</entry>
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    <accession>A0A673BZ79</accession>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="Fork_head_dom"/>
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        <property type="entry name" value="FORKHEAD BOX I2-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
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        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="146"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="146"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
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        <location>
            <begin position="262"/>
            <end position="306"/>
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        <location>
            <begin position="9"/>
            <end position="24"/>
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        <location>
            <begin position="297"/>
            <end position="306"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50039"/>
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            <dbReference type="Ensembl" id="ENSSORP00005047677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="415" mass="44827" checksum="B70C315C3678EAB8" modified="2020-06-17" version="1">MNSIDPQSHHHQHHQNHQNQHHHTSPTVGSLPPKGAQEAPDMAAVYCENFSSMYHHQQSLQGAQRPPGGYGLGDYASSPNPYLWLNGPPGVNSSASAYLHGNNPASFIPPSYGSQRQFLTNSPGFGGPDLGWLSIASQEELLKLVRPPYSYSALIAMAIQNAHEKKLTLSQIYQYVADNFPFYKKSKAGWQNSIRHNLSLNDCFKKVPRDEDDPGKGNYWTLDPNCEKMFDNGNFRRKRKRRSDPSGAGGVAVAAVGATKVEDGRPAAGAPLKPSDSPQLLGPSSPEMEAMNDNHKGSTSSLSPPGLASAAAAAPCFNNFYSSMSTLSSGAPSRPQGSLGLVNELSNRNITALSPYHANHGGQDTGASEHGEGLHFNRGVYYNTFGSGQSGQFNSHFYNSFSVNSLIYPREGAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5A3</accession>
    <name>A0A673C5A3_9TELE</name>
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        <submittedName>
            <fullName evidence="9">Zgc:158316</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fam126b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Hyccin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C5A3"/>
    <dbReference type="FunCoup" id="A0A673C5A3">
        <property type="interactions" value="1016"/>
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        <property type="protein sequence ID" value="ENSSORP00005050591.1"/>
        <property type="gene ID" value="ENSSORG00005022890.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C5A3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein localization to plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Hyccin"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF09790">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025473578" evidence="8">
        <location>
            <begin position="23"/>
            <end position="553"/>
        </location>
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            <begin position="355"/>
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            <begin position="398"/>
            <end position="414"/>
        </location>
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        <location>
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        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
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            <begin position="537"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="553" mass="61649" checksum="4D9B1EA447425C83" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C8D2</accession>
    <name>A0A673C8D2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115438561</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C8D2"/>
    <dbReference type="Ensembl" id="ENSSORT00005053084.1">
        <property type="protein sequence ID" value="ENSSORP00005051846.1"/>
        <property type="gene ID" value="ENSSORG00005023410.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C8D2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="13"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="130"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="171"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30601" checksum="C35E22FC39849838" modified="2020-06-17" version="1">MHTQKHMADVPKCCICLDEFTNPASLPCGHCFCLSCIGEYFRIHGACQCPLCKTFFPTWPQLKTDQTLHTERASAPLRAGEVTCDFCSAQRRAVKSCLKCLASYCDSHLQPHYQSEELGRHLLISVDKNLEDSVCRLHGRQLGRFCSSDQTCVCVMCAQTDHRGHRIISITKEAAKKKVKLKRKRMKLQQTLQEKLSKIEKLRLSMNLSHDDQKETQSHHKELIKELEEDISELQMRNDELEQLSHVEDSLHFLQVRQSHGKTQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C8U3</accession>
    <name>A0A673C8U3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C8U3"/>
    <dbReference type="Ensembl" id="ENSSORT00005049795.1">
        <property type="protein sequence ID" value="ENSSORP00005048603.1"/>
        <property type="gene ID" value="ENSSORG00005022146.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C8U3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052749">
        <property type="entry name" value="Alpha-tectorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160">
        <property type="entry name" value="ALPHA-TECTORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160:SF9">
        <property type="entry name" value="PROTEIN PRY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFD" evidence="1">
        <location>
            <begin position="53"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="14048" checksum="7A48CF9FAD4D184C" modified="2020-06-17" version="1">QGGEQFWVGDECQSLCSCNGITGLVHCVPNSCGAQESCRVVGGEYGCHPSPHGTCSASGDPHYLTFDQKAYDFQGTCRYVLATLCNDTTGLHQFSVEAKNEAWQGLPVSITAEVFVNVWGYRVHIRENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAQ1</accession>
    <name>A0A673CAQ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MALT paracaspase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">malt1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030143436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CAQ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005051583.1">
        <property type="protein sequence ID" value="ENSSORP00005050374.1"/>
        <property type="gene ID" value="ENSSORG00005022843.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425720"/>
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    <dbReference type="InParanoid" id="A0A673CAQ1"/>
    <dbReference type="OrthoDB" id="412369at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Mucosa-associated lymphoid tissue lymphoma translocation protein 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052039">
        <property type="entry name" value="Caspase-related_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037940">
        <property type="entry name" value="MALT1_Death"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041077">
        <property type="entry name" value="MALT1_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033540">
        <property type="entry name" value="MALT1_IG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576">
        <property type="entry name" value="MUCOSA ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION PROTEIN 1/PARACASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576:SF40">
        <property type="entry name" value="MUCOSA-ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18703">
        <property type="entry name" value="MALT1_Ig"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="107"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="208"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="1">
        <location>
            <begin position="330"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="828" mass="92640" checksum="5B7C5675F27CA750" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CB31</accession>
    <name>A0A673CB31_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat domain 44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ankrd44</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CB31"/>
    <dbReference type="FunCoup" id="A0A673CB31">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050544.1">
        <property type="protein sequence ID" value="ENSSORP00005049343.1"/>
        <property type="gene ID" value="ENSSORG00005019194.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CB31"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF82">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 6 REGULATORY ANKYRIN REPEAT SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ank"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="73"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="172"/>
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        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="238"/>
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        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="371"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="419"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="452"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="576"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="609"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="643"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="679"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="783"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="850"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="883"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="917"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="953"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1025"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1040"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1062"/>
            <end position="1072"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1072" mass="114441" checksum="8846BB49A612CF1A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBV5</accession>
    <name>A0A673CBV5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-interferon-inducible lysosomal thiol reductase</fullName>
            <ecNumber evidence="7">1.8.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-interferon-inducible protein IP-30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115417468</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Lysosomal thiol reductase that can reduce protein disulfide bonds. Facilitates the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Dimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="EC" id="1.8.-.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673CBV5"/>
    <dbReference type="FunCoup" id="A0A673CBV5">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052792.1">
        <property type="protein sequence ID" value="ENSSORP00005051560.1"/>
        <property type="gene ID" value="ENSSORG00005023286.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBV5"/>
    <dbReference type="OrthoDB" id="958254at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003119">
        <property type="entry name" value="SAP_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF45">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02199">
        <property type="entry name" value="SapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51110">
        <property type="entry name" value="SAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025569688" description="Gamma-interferon-inducible lysosomal thiol reductase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin A-type" evidence="9">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="257" mass="28350" checksum="3A1C88E37ACA09D3" modified="2020-06-17" version="1">MKLPGLPAVVLFLCIVQEICGSHPKPSCHYPPSQWCRSLEIAIQCKVQKQCMELWSVRPEPAAPPVSVSLYYESLCPACRSFLTQQLFPTWTMLQDIMTVTLVPYGNAKEIPSANSPFTCQHGALECTGNMIEACVLHLTHPSTAFHIIYCMESAVDVIAAAEPCLRLHDPGVSWSSVQSCVNGTVGKRLMHRHAAMTRALDPGHTYVPWVTVDGEYTKQTEDQALSSLFQLVCQLYKGVKPPTCTGAPVRLNRGFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CEM5</accession>
    <name>A0A673CEM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">LIM domain kinase 2</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">limk2</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030000293.1">
        <property type="nucleotide sequence ID" value="XM_030144433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CEM5"/>
    <dbReference type="FunCoup" id="A0A673CEM5">
        <property type="interactions" value="1299"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055297.1">
        <property type="protein sequence ID" value="ENSSORP00005054026.1"/>
        <property type="gene ID" value="ENSSORG00005023537.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426361"/>
    <dbReference type="CTD" id="3985"/>
    <dbReference type="InParanoid" id="A0A673CEM5"/>
    <dbReference type="OrthoDB" id="20134at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09463">
        <property type="entry name" value="LIM1_LIMK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09465">
        <property type="entry name" value="LIM2_LIMK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06754">
        <property type="entry name" value="PDZ_LIMK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000038">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000038">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000197">
        <property type="entry name" value="LIM domain kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000082">
        <property type="entry name" value="LIM domain kinase 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF1">
        <property type="entry name" value="LIM DOMAIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 20" id="KW-0440">LIM domain</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 20" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="24">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="24">
        <location>
            <begin position="70"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="25">
        <location>
            <begin position="151"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="334"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="626"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="653"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="662" mass="74742" checksum="7334C599E6994827" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CF37</accession>
    <name>A0A673CF37_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CF37"/>
    <dbReference type="Ensembl" id="ENSSORT00005053168.1">
        <property type="protein sequence ID" value="ENSSORP00005051929.1"/>
        <property type="gene ID" value="ENSSORG00005023445.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CF37"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="61"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="197" mass="23195" checksum="32593F856723BC8F" modified="2020-06-17" version="1">MELQKSNGNLERSNQNLTKRSRLLEDEKTNLTIERDQLEVFMFVLVIFVDVSSCPDSWRKFGGHCYFISNSKKTWHVSKQDCENRGATLAVVSNEEEQRFISSLKRRLWIGLNDLEEEGVWKWVNGENVVCLYLFCRYWKRGQPDNCCRGEDCGELLGQEKDPLINWNDRSCKQEEFFICEKAFHEDTDPALHRPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFM3</accession>
    <name>A0A673CFM3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Neuronal growth regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">negr1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the immunoglobulin superfamily. IgLON family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029988244.1">
        <property type="nucleotide sequence ID" value="XM_030132384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CFM3"/>
    <dbReference type="Ensembl" id="ENSSORT00005054184.1">
        <property type="protein sequence ID" value="ENSSORP00005052930.1"/>
        <property type="gene ID" value="ENSSORG00005023845.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418064"/>
    <dbReference type="CTD" id="257194"/>
    <dbReference type="InParanoid" id="A0A673CFM3"/>
    <dbReference type="OrthoDB" id="6159398at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000305">
        <property type="entry name" value="neurotrimin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050876">
        <property type="entry name" value="IgLON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757">
        <property type="entry name" value="IGLON FAMILY OF IMMUNOGLOBULIN SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757:SF6">
        <property type="entry name" value="NEURONAL GROWTH REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025437377" description="Neuronal growth regulator 1" evidence="14">
        <location>
            <begin position="34"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="33"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="139"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="225"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="345" mass="37307" checksum="147977D5FB6EA7E7" modified="2020-06-17" version="1">MDIMIAVQDACVSGQWLTAIILSLCCFLPSCLPAGQTVDYSSVESVVSRQGDTALLRCYLLDGISKGAWLNRSSIIFAGNDKWSVDPRVSIVSSVGDKHEYSLQIQRVDVTDDGQYTCSIQSERNPRPKLLNLIVKVPPKIYDISSDINVNEGSNVSLICTASGKPEPAISWRHITPLARKYDSGEYLNITGITRDQAGDYECSAINDIASPDTKTVKVTVNFAPSIHEMKSHGVGLGRTALLRCEAAAVPSPTFEWYKGEKRIIKGQGIDIKSLSSRSVLTVNNMTEDRYGNYTCVASNKLGTANASVPLIPPNTAPYGSTGSAPVPLACQYLVLALSSFISVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGF0</accession>
    <name>A0A673CGF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Integral membrane protein GPR137</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CGF0"/>
    <dbReference type="Ensembl" id="ENSSORT00005054470.1">
        <property type="protein sequence ID" value="ENSSORP00005053215.1"/>
        <property type="gene ID" value="ENSSORG00005023940.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGF0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045779">
        <property type="term" value="P:negative regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029723">
        <property type="entry name" value="GPR137"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146:SF0">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR137B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21272" checksum="CBEE15814136FBB9" modified="2025-10-08" version="2">MRTSSSARVRTRWPMAAPPDPPVPPPDPPVPPPTLGPAVRPFVTLGLTGVYTAFYALLFLFVYAQLWLVLRYRHKRVSFQTGFLFLCLVWAALRTLLFSFYFRNSRTVNSLGPVPFWSLYCLPVCLQFWTLSLMNLYCAQVGPGPGPGPGLGPGLGRLRGGLYESGSSGMHGLGGSDVVYRVLGRGGLMWSTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CI94</accession>
    <name>A0A673CI94_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 231</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115421460</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transmembrane component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM231 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CI94"/>
    <dbReference type="FunCoup" id="A0A673CI94">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055180.1">
        <property type="protein sequence ID" value="ENSSORP00005053910.1"/>
        <property type="gene ID" value="ENSSORG00005024206.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CI94"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019306">
        <property type="entry name" value="TMEM231"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14605">
        <property type="entry name" value="CHST5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14605:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 231"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10149">
        <property type="entry name" value="TM231"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="302" mass="34665" checksum="0A2F3B0F33E58190" modified="2020-06-17" version="1">MAFYEVYSHPALIRYKTSVCTKATLFLVVVVCLTYIPPLLVAYRSQGFWIKRSTYEEQPVVRFQYQTLLVAATSTDGDYVAWSTFPHLNNMLGSRLRIPSVSVREEDKNQDGKLDLLNFQLELPLKPDEQVYSIQLLLTFSYQLFRMSTVVMQSLAYVQYSSSVPGSKLFINGDLRLQQRTPLPHRGLYNIYNVSVIDGSSPFAASYDLENIIQMYQDRNLSTVLSCPMPVWTVGRAAASPFQLKAQIRYPLELITYPSDQTFTCQLVLLIFLWVFERIQRFVFQNQVLTTVPIPLGKPHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJR9</accession>
    <name>A0A673CJR9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Reticulon 4 receptor</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the Nogo receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJR9"/>
    <dbReference type="Ensembl" id="ENSSORT00005057129.1">
        <property type="protein sequence ID" value="ENSSORP00005055841.1"/>
        <property type="gene ID" value="ENSSORG00005024895.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJR9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905573">
        <property type="term" value="F:ganglioside GM1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905576">
        <property type="term" value="F:ganglioside GT1b binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000018">
        <property type="entry name" value="Reticulon 4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF174">
        <property type="entry name" value="RETICULON-4 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025389474" evidence="17">
        <location>
            <begin position="33"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="353"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="470" mass="53121" checksum="BC957B1863EE464E" modified="2020-06-17" version="1">MGRGDTMFGAQTKGGRLLFLMVWLNLVPQTDSCPAKCVCYSEPRPTVACQQQGLFSIPTEIPVRSQRIFLQSNKLTVVRSTSFSSCHNLTVLWLYSNNISYIEAGAFYGLEKLEELDIGDNSNLRTISPTAFRGLTKLHTLHLHRCGLSELPVGVFRGMFSLQYLYLQDNNIHTLHDDTFLDLANLTYLYLHNNKIKIVTDNMFRGLINLDRLLLHQNRVIYVQPRAFTDLGKLKTLYLFYNNLTVLTGETMDPLATLQYLRLNGNQWICDCRARTLWEWFKRFKGSSSELECNIPEFLAGEDLKALKSEDLEGCVKKFQIQTDLFSSKTQSGKFPSSENPLGDTIPRCCLGDNDKSSILSGKSRQNNPLKEKENMSKTKFKEPERTKNETQNKQNDGPLGTLSNALDNSSNLNPDLIDTLESSTASNKKKKKCVKKPKSDAQCIKGQGSTLQVLRSLIIPMIWISLAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLB9</accession>
    <name>A0A673CLB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">lbx1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CLB9"/>
    <dbReference type="FunCoup" id="A0A673CLB9">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054095.1">
        <property type="protein sequence ID" value="ENSSORP00005052843.1"/>
        <property type="gene ID" value="ENSSORG00005023809.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLB9"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000098">
        <property type="entry name" value="Transcription factor LBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051892">
        <property type="entry name" value="LBX_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336">
        <property type="entry name" value="TRANSCRIPTION FACTOR LBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR LBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="124"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="126"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="271" mass="30229" checksum="99E16C3EAFC98988" modified="2020-06-17" version="1">MMTSKEVAKCDAVENRRRSPLDHLPPPANSNKPLTPFSIEDILNKPSVKRSYTICGTAHLISSSEKHRPSSIPLSSRALLTQTSPLCALEELASKTFKGLEVSVLQAAEGRDGMTLFGQRSTPKKRRKSRTAFTNHQIYELEKRFLYQKYLSPADRDQIAQQLGLTNAQVITWFQNRRAKLKRDLEEMKADVESAKAVGQVPLDKLAKLADLEKCANGTLGHPRAESPARCGQQELELAQKLRSSPLSPFSDHTTSKECSEDEDVEIDVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CNE9</accession>
    <name>A0A673CNE9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">KASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus outer membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nesprin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CNE9"/>
    <dbReference type="Ensembl" id="ENSSORT00005057066.1">
        <property type="protein sequence ID" value="ENSSORP00005055780.1"/>
        <property type="gene ID" value="ENSSORG00005024861.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CNE9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000157">
        <property type="entry name" value="Nesprin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012315">
        <property type="entry name" value="KASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056887">
        <property type="entry name" value="SYNE1/2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF4">
        <property type="entry name" value="NESPRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10541">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25035">
        <property type="entry name" value="SYNE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01249">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51049">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="761"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="9">
        <location>
            <begin position="789"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="KASH" evidence="12">
        <location>
            <begin position="759"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="399"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="399"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="818" mass="93830" checksum="514741FA4B411916" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CPX4</accession>
    <name>A0A673CPX4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WD repeat, SAM and U-box domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115412146</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CPX4"/>
    <dbReference type="Ensembl" id="ENSSORT00005059005.1">
        <property type="protein sequence ID" value="ENSSORP00005057686.1"/>
        <property type="gene ID" value="ENSSORG00005025546.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CPX4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052085">
        <property type="entry name" value="WD-SAM-U-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46573">
        <property type="entry name" value="WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46573:SF1">
        <property type="entry name" value="WD REPEAT, SAM AND U-BOX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="264"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="334"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="6">
        <location>
            <begin position="402"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="473" mass="51831" checksum="1A9AA7DBB829E1C2" modified="2020-06-17" version="1">MVSIVRTLRDHTDEVSCCAFSSALLASCSGDKTLRVYHTSDLSELPFSPLSSHGYRVHCCCFSSCGRFLVSCSTDGSAIVWNSDTGERSAALQHPSRSPLRVCALAHDCAHLLAGACDGTVALWDFSSRTLRRCSAVSEDSVVACCFSPSNQMFVTGCTRGDLKLWDLDVNLLHAEKDAHDLGVTCCSFAAQFNVGVYGCCVEFRLASCGQDSQLKIWIVSQREGAGCVMKLLHTLTAQSAPVLSCAFSSDGELVNLGTVLHTLNQHDRYVTAVALSPTMPWIATGSMDRTVTVWRIGDGDDTTGKYSEQTWFSQTDGEERKLPGHSRLLLADWSEEDVQNWLSEEGLEELVSIFKTNNIDGPELNQMSKETAAELGIESVGLRGRLLRKIEALKAEQSGSEAPDEFLCPITRELMRDPVIAADGYSYERESIQSWIRGKNKTSPMTNLPLQTTLLTPNRSLKTAIARWKSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CT34</accession>
    <name>A0A673CT34_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="36">Trifunctional enzyme subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="35">1.1.1.211</ecNumber>
            <ecNumber evidence="6">4.2.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="37">Monolysocardiolipin acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="38">TP-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="44" type="primary">hadhaa</name>
    </gene>
    <organism evidence="44 45">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="44" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="44" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="44" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(3S)-3-hydroxydodecanoyl-CoA + NAD(+) = 3-oxododecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62558"/>
            <dbReference type="ChEBI" id="CHEBI:62615"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:31180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3S)-3-hydroxydodecanoyl-CoA = (2E)-dodecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31075"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:62558"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="29">
            <dbReference type="Rhea" id="RHEA:31077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(3S)-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62548"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:31188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(3S)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31159"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:31160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(3S)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:31165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3S)-hydroxyoctanoyl-CoA + NAD(+) = 3-oxooctanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31195"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62617"/>
            <dbReference type="ChEBI" id="CHEBI:62619"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:31196"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(3S)-hydroxyoctanoyl-CoA = (2E)-octenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31199"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:62617"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="27">
            <dbReference type="Rhea" id="RHEA:31201"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(3S)-hydroxytetradecanoyl-CoA + NAD(+) = 3-oxotetradecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62543"/>
            <dbReference type="ChEBI" id="CHEBI:62614"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:31168"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + (9Z)-octadecenoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43676"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83582"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:43677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + (9Z,12Z)-octadecadienoyl-CoA = 1',3'-bis-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43672"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83581"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:43673"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + hexadecanoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-hexadecanoyl-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43680"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:43681"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:16105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:16107"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:20724"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:137480"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:20726"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a long-chain (3S)-3-hydroxy fatty acyl-CoA + NAD(+) = a long-chain 3-oxo-fatty acyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:136757"/>
            <dbReference type="ChEBI" id="CHEBI:136758"/>
            <dbReference type="EC" id="1.1.1.211"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:52657"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="34">Heterotetramer of 2 alpha/HADHA and 2 beta/HADHB subunits; forms the mitochondrial trifunctional enzyme. Also purified as higher order heterooligomers including a 4 alpha/HADHA and 4 beta/HADHB heterooligomer which physiological significance remains unclear. The mitochondrial trifunctional enzyme interacts with MTLN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="41">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.211" evidence="35"/>
    <dbReference type="EC" id="4.2.1.17" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673CT34"/>
    <dbReference type="FunCoup" id="A0A673CT34">
        <property type="interactions" value="1210"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057003.1">
        <property type="protein sequence ID" value="ENSSORP00005055717.1"/>
        <property type="gene ID" value="ENSSORG00005024365.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CT34"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016507">
        <property type="term" value="C:mitochondrial fatty acid beta-oxidation multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016509">
        <property type="term" value="F:long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000011">
        <property type="entry name" value="Fatty acid oxidation complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.50:FF:000002">
        <property type="entry name" value="Trifunctional enzyme subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012803">
        <property type="entry name" value="Fa_ox_alpha_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050136">
        <property type="entry name" value="FA_oxidation_alpha_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02441">
        <property type="entry name" value="fa_ox_alpha_mit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43612">
        <property type="entry name" value="TRIFUNCTIONAL ENZYME SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43612:SF3">
        <property type="entry name" value="TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-0456">Lyase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="43">
        <location>
            <begin position="363"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="42">
        <location>
            <begin position="544"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="active site" description="For hydroxyacyl-coenzyme A dehydrogenase activity" evidence="39">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <feature type="site" description="Important for long-chain enoyl-CoA hydratase activity" evidence="40">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="site" description="Important for long-chain enoyl-CoA hydratase activity" evidence="40">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="site" description="Important for hydroxyacyl-coenzyme A dehydrogenase activity" evidence="40">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00035854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00050446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00052711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00052834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00052860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00052945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00052989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00062153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00066806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00068347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00077617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00083277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="PIRSR" id="PIRSR612803-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="PIRSR" id="PIRSR612803-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="42">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="763" mass="81744" checksum="EB2524E1C3A11095" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CUZ5</accession>
    <name>A0A673CUZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">26S proteasome regulatory subunit p27</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmd9</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with PSMC3. Part of a transient complex (modulator) containing PSMD9, PSMC6 and PSMC3 formed during the assembly of the 26S proteasome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000050.1">
        <property type="nucleotide sequence ID" value="XM_030144190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CUZ5"/>
    <dbReference type="FunCoup" id="A0A673CUZ5">
        <property type="interactions" value="1913"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057095.1">
        <property type="protein sequence ID" value="ENSSORP00005055808.1"/>
        <property type="gene ID" value="ENSSORG00005024875.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426190"/>
    <dbReference type="CTD" id="5715"/>
    <dbReference type="InParanoid" id="A0A673CUZ5"/>
    <dbReference type="OrthoDB" id="72325at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000107">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="110"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="210" mass="23140" checksum="B90FEE8F4AE1EFFA" modified="2020-06-17" version="1">MRTTGEASAGNSDLTMDDVKNLIKKKDEIEEQIKAYYDVLEDQGVGVAGPLVDAEGYPRADVNLYQIRSARHNISCLQNDHKAIMAEIEEALHKLHAQEKAKREKDESEAREEAQQATQPPPFARVDTVTQGSPACGAGLRVGDELTEFGSVNSENFQNLQNIASVVQHSEGKPLRVMVIRGGQRVHMTLTPQRWSGRGLLGCNIVPIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CVG5</accession>
    <name>A0A673CVG5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Synaptophysin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sypl2b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CVG5"/>
    <dbReference type="Ensembl" id="ENSSORT00005058816.1">
        <property type="protein sequence ID" value="ENSSORP00005057498.1"/>
        <property type="gene ID" value="ENSSORG00005025484.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058817.1">
        <property type="protein sequence ID" value="ENSSORP00005057499.1"/>
        <property type="gene ID" value="ENSSORG00005025484.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10006326at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF9">
        <property type="entry name" value="SYNAPTOPHYSIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00220">
        <property type="entry name" value="SYNAPTOPHYSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="19"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="244" mass="26633" checksum="9A81A4EAB548D40B" modified="2020-06-17" version="1">MESVAQKVLTSGFTLDLGPLKEPLAFIRLLEWVFAIFAFATTGGYSGSTHVNIQCHGTTATEIQAAFAYPFRLMAHPYEVPDCVINKTTTSKFYLTGNHSSSAEFYVCIGVFAFLYSTATLVIYLGYQHVYRGSNRGPVVDLLVTATFAFLWLVSSSAWAKGLTDVKWATSPTTIVSLLAVCKDSANKCTPGAMPHMGRLNASVIFGFLNLILWGGNCWFIYKETPFHKSANQPADAEAGVRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6P7</accession>
    <name>A0A672Y6P7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Arginine/serine-rich coiled-coil 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y6P7"/>
    <dbReference type="Ensembl" id="ENSSORT00005002049.1">
        <property type="protein sequence ID" value="ENSSORP00005001993.1"/>
        <property type="gene ID" value="ENSSORG00005001236.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y6P7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="200" mass="22466" checksum="97DCDDDFD0D849AF" modified="2020-06-17" version="1">MTQPTSVEQRSMDVCWIDRLRDGRVISDILDCMCQRVLYREITYNQLSPVQMKSGAFQNMTRDGWPSVGLCTIMCVCVSGHSCACFCSSAPICVTCVSEGTSLEEQVRRIKDIEAIESDSFVPQAFKSSRDDIKVESSEVKQESEKADCLGVSLPMSIVYNDSDTLAHPSLFMDKEKAEELWLNRLISLRQERLMGSPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8E9</accession>
    <name>A0A672Y8E9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MAPK regulated corepressor interacting protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mcrip2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCRIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y8E9"/>
    <dbReference type="FunCoup" id="A0A672Y8E9">
        <property type="interactions" value="1398"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003068.1">
        <property type="protein sequence ID" value="ENSSORP00005002977.1"/>
        <property type="gene ID" value="ENSSORG00005001849.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8E9"/>
    <dbReference type="OrthoDB" id="9983138at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029428">
        <property type="entry name" value="MCRIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14799">
        <property type="entry name" value="FAM195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="143" mass="16147" checksum="B629E15D9D91466E" modified="2020-06-17" version="1">MMYTITRGPSKLVTQRRTGPTQQIESKFSDLKLKPTSWLTSNSPPPKIVFNRLNGKRYHGAATQKSNSAAEGFTPAHEENVRFVYEAWQEVEQKLGNDGETTGSQGPIQYSEKTPNSAMKNFVPIDLEEWWAQRFLANIANLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAK8</accession>
    <name>A0A672YAK8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Golgin subfamily A member 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">golga7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ERF4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YAK8"/>
    <dbReference type="Ensembl" id="ENSSORT00005000115.1">
        <property type="protein sequence ID" value="ENSSORP00005000100.1"/>
        <property type="gene ID" value="ENSSORG00005000081.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAK8"/>
    <dbReference type="OrthoDB" id="2190159at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002178">
        <property type="term" value="C:palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF1">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="9">
        <location>
            <begin position="42"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="167" mass="19187" checksum="AE41ECBB00685856" modified="2020-06-17" version="1">MVQDWRKLEVVDRCVADSYETPTMAETHSLQDLQQPAVSSKVFVQRDYSSGTICKFQTKFPSELESRVDKQQFEETIQTLNNLYAEAEKLGGKSYLEGCLACLTAYTIFLCMETHYEKVLKKIARYIKDQNEKIYAPRGLLLTDPIERGLRVVEITIFEDRSIGSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAZ4</accession>
    <name>A0A672YAZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase SH3RF1</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Plenty of SH3s</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase SH3RF1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">SH3 domain-containing RING finger protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">sh3rf1</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SH3RF family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A672YAZ4"/>
    <dbReference type="FunCoup" id="A0A672YAZ4">
        <property type="interactions" value="573"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000399.1">
        <property type="protein sequence ID" value="ENSSORP00005000375.1"/>
        <property type="gene ID" value="ENSSORG00005000239.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAZ4"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16748">
        <property type="entry name" value="RING-HC_SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11927">
        <property type="entry name" value="SH3_SH3RF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11930">
        <property type="entry name" value="SH3_SH3RF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11926">
        <property type="entry name" value="SH3_SH3RF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11785">
        <property type="entry name" value="SH3_SH3RF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000077">
        <property type="entry name" value="E3 ubiquitin-protein ligase SH3RF1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000063">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000091">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000118">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000001">
        <property type="entry name" value="Sorbin and SH3 domain-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035816">
        <property type="entry name" value="SH3RF1/SH3RF3_SH3_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035795">
        <property type="entry name" value="SH3RF1_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF44">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 24" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
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        <source>
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    <sequence length="856" mass="90709" checksum="9D9B82F3FCC49B2A" modified="2020-06-17" version="1"> </sequence>
</entry>
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    <accession>A0A672YCP3</accession>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF30</accession>
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    <protein>
        <submittedName>
            <fullName evidence="7">Si:dkeyp-72e1.7</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="primary">mpv17l</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003338.1">
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        <property type="gene ID" value="ENSSORG00005001977.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YF30"/>
    <dbReference type="Proteomes" id="UP000472271">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF39">
        <property type="entry name" value="MPV17-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="216" mass="25063" checksum="6C36E2BDED6BEF21" modified="2020-06-17" version="1">MRRAVLKEAAKRFPWLANVTLYGCLFAGGDLVHQLLAQKERMDWKHTRNVAIVAISFHGNFNYFWLRALERRFPGKSAGMVFRKLLLDQSFASPLGTSVFYTGVSFLEGKEDVFEDWREKFFNTWKTGLMYWPFMQFLNFALMPLHMRTAFMGCCAFLWATFLCFSRQNGDGTAAVALAFIMDPRKTLEEIFDSNYCFIILFFITGLKPTLTTQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJK9</accession>
    <name>A0A672YJK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tripartite motif containing 35-13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YJK9"/>
    <dbReference type="Ensembl" id="ENSSORT00005004900.1">
        <property type="protein sequence ID" value="ENSSORP00005004761.1"/>
        <property type="gene ID" value="ENSSORG00005002884.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJK9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12893">
        <property type="entry name" value="SPRY_PRY_TRIM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="93"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="282"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="134"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="53716" checksum="1A8E170D52E1109D" modified="2020-06-17" version="1">MAEKKIEELESFLSCHVCSETFRDPVSLSCNHRFCSNCLKEFWEQTKNENCPICKRKSSKDFLIVDFALKELADSFAGRLNVGSSETEIGQKKVEVVCSKHQEEPKLFCKDENRAVCSICDVSVHDNHTVVPVEEAVTEMKDELKSDLKSLQEKKKKYEETQKTYNEIMKLSKKQVLFTENQIRAEFNKLQQFLKEEEESRLAALREEEEQKRRSVIREMKRIEEQMSSVSDSICAVEEELQKASAPFLISCKDTQSRARAQCSVSEPQLISGALIHVDKHLGNLSFRVWDKMRDKVHFSPVILDPNTAHGRLHLSDDLTSVRYGDTKQHLPDNPERFTNYADVHGSEGFNSGKHSWDVEVGDHPEWTLGVVKESVHRQGERFVSPEDGIWCLKHHSGKYINGLQQTVTVQTSPQRIRVELDYDRGEVSFYNAEDKTHIYTQIGCFLEKLYPYFSIGQAVDAKFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJR8</accession>
    <name>A0A672YJR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJR8"/>
    <dbReference type="Ensembl" id="ENSSORT00005004928.1">
        <property type="protein sequence ID" value="ENSSORP00005004789.1"/>
        <property type="gene ID" value="ENSSORG00005002900.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJR8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="92"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="133"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="39299" checksum="DE03B35C75311774" modified="2020-06-17" version="1">MAEKKIELVEHYLTCPVCLETFRDPVSLSCNHSFCSNCLKEFWKENENRTCPICRRKNSKHIFVNFTLKELADSFAGRLNIGSSETEKGEKKVEVVCSKHQEEPKLFCKDENRAVCSICDVSFHDTHTVVPVEEAVTEMKDELKSDLKSLQEKKNKYEETQKTYNEIIQHLKKQVLFTENQIRAEFNKLQQFLKEEEESRLAALREEEEQKRRSVIREMKRIEEQMSSVSDSICAVEEELQKASAPFLISCKDTQSRARAQCSVSEPQTINVCKHMEKNWPSFLSEKCIQILLLFLWLITHRWSLTHSQTFLRFRPVFQLRLTLQTVVFFANFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLH9</accession>
    <name>A0A672YLH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rbm45</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030011208.1">
        <property type="nucleotide sequence ID" value="XM_030155348.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030011210.1">
        <property type="nucleotide sequence ID" value="XM_030155350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YLH9"/>
    <dbReference type="FunCoup" id="A0A672YLH9">
        <property type="interactions" value="1679"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005592.1">
        <property type="protein sequence ID" value="ENSSORP00005005434.1"/>
        <property type="gene ID" value="ENSSORG00005003229.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 15"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
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        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="98"/>
        </location>
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        <location>
            <begin position="113"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="243"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="391"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="195"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="475" mass="52883" checksum="D2C677D3659024CA" modified="2020-06-17" version="1">MDENSSKQIPENVEDPPNSRLFVVTSRSITEEDLRESFSPFGEIQGIWVLKDKQTKESKGVSYVKFAKSSQACLAMEEMHGKVVVDETKPIKVFIAQSRSSTRHRDVEDEELTRIFVMIPKTFTEEDLQNTFKEYGDIEYCVIIKNKFTGESKGLGYVRYYKPSQAALAIENCDKTYRAILAEPRSKVSTADEYSGGGGGGGGGGNRGGDYMSGADSMNQYSYPMADPSGNYSLMDYRSSDITRCLMMSTRAALTQEQIFALFDLIPGMEYCELQRDAYGMSKGHALIRYSNLGSAVYAKEKLNGFEYPPGNRLAVNFVDDGEDRSSPVGRMAMQFVASQMMSSVWNGPSTSQGMKQPMAFSGVSPMSRVQTDVSLPPLKKLAPPESKTKERLFVVFSPSPLPQDILEDVFCRFGSLIEVHLVPGRKVGYMKYAEKQCADDAMAALHGRIVNGVKMKVLLADPPREESHKRPRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMC1</accession>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <authorList>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X3"/>
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    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="123" mass="13907" checksum="3E5D19C0735E7439" modified="2020-06-17" version="1">MRKDSPWKQNVSLAILSSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQPYPTDITGQLNLSDPSVSTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPJ2</accession>
    <name>A0A672YPJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G0/G1 switch 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="component" value="Chromosome 7"/>
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        <property type="entry name" value="G0S2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15570">
        <property type="entry name" value="G0/G1 SWITCH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15570:SF2">
        <property type="entry name" value="G0_G1 SWITCH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15103">
        <property type="entry name" value="G0-G1_switch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9175" checksum="070E203F86133209" modified="2020-06-17" version="1">METIGEIIPFAKEILNQKPSRGMLKIYMFGTTLAMLGLVGGLMETVCQQFAEDDVADVHTPLVDATHLMVTRRRSSANRLHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSG9</accession>
    <name>A0A672YSG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">psmb10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672YSG9"/>
    <dbReference type="FunCoup" id="A0A672YSG9">
        <property type="interactions" value="1225"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007813.1">
        <property type="protein sequence ID" value="ENSSORP00005007533.1"/>
        <property type="gene ID" value="ENSSORG00005004293.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YSG9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024689">
        <property type="entry name" value="Proteasome_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12465">
        <property type="entry name" value="Pr_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="697" mass="76187" checksum="F7DDA8EA68B90F5E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWS6</accession>
    <name>A0A672YWS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyroglutamyl-peptidase I</fullName>
            <ecNumber evidence="5">3.4.19.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.</text>
            <dbReference type="EC" id="3.4.19.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.3" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672YWS6"/>
    <dbReference type="Ensembl" id="ENSSORT00005009492.1">
        <property type="protein sequence ID" value="ENSSORP00005009186.1"/>
        <property type="gene ID" value="ENSSORG00005005040.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YWS6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033693">
        <property type="entry name" value="PGPEP1_Glu_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01333">
        <property type="entry name" value="PYRASE_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="145" mass="15722" checksum="0DE31FE4E1B787F1" modified="2020-06-17" version="1">AYFLMKLRNTTAFAPGTKISSVIFSFAFSSQELKKLGLGSEVDLQVYEVPVEYQTVQSLVPSLWKQYHPLLVVHVGVSGMTATVTLEKCGRNHGYKGPDCIDSVLDMESVAKRVTASGLGVAVSVSKDAGSTPYPGLNSLQQHWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYC4</accession>
    <name>A0A672YYC4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">adenylate cyclase</fullName>
            <ecNumber evidence="4 16">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115417444</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="18">Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 19">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4 16"/>
    <dbReference type="AlphaFoldDB" id="A0A672YYC4"/>
    <dbReference type="Ensembl" id="ENSSORT00005009889.1">
        <property type="protein sequence ID" value="ENSSORP00005009570.1"/>
        <property type="gene ID" value="ENSSORG00005005227.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYC4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000001">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000002">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030672">
        <property type="entry name" value="Adcy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009398">
        <property type="entry name" value="Adcy_conserved_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF22">
        <property type="entry name" value="ADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06327">
        <property type="entry name" value="Adcy_cons_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039050">
        <property type="entry name" value="Ade_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 16" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 16 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 16 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="153"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="601"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="628"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="661"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="704"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="730"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="21">
        <location>
            <begin position="295"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="21">
        <location>
            <begin position="846"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="300"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="342"/>
            <end position="344"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="898"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="975"/>
            <end position="977"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="982"/>
            <end position="986"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="1021"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR039050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR039050-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR039050-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1090" mass="122172" checksum="83BE98D1288098F5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYF8</accession>
    <name>A0A672YYF8_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">zfand2a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030142196.1"/>
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        <property type="protein sequence ID" value="ENSSORP00005009620.1"/>
        <property type="gene ID" value="ENSSORG00005005255.1"/>
    </dbReference>
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    <dbReference type="CTD" id="90637"/>
    <dbReference type="InParanoid" id="A0A672YYF8"/>
    <dbReference type="OrthoDB" id="431929at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
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        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
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        <property type="entry name" value="ZINC FINGER AN1-TYPE CONTAINING 2A-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_AN1"/>
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        <location>
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            <end position="52"/>
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        </source>
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        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="279" mass="30842" checksum="4CAEEF383391CE41" modified="2020-06-17" version="1">MEFPDLGEHCSEKSCKRLDFLPMRCDACQEIFCKDHITYANHKCTSSYKKDVQVPVCPLCNTPIPIKRGEMPDIKVGEHIDRDCKSDPAQRKRKIFTNKCSRGGCKQKEMIRVTCDQCHLNYCLKHRHPLDHDCKTDGKPLSKSGHAAVMRNQGASSTSASSSSSSSSGNTRPVSNGVSASNRNINSGTTQRIPTSVSAQNVIPPSASFQAGMTEEQALQRALEMSLAEARQPVQPTLSPQEQEDLALAQALAASEEEYRRQQQRQQGRESKQSNCSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3S9</accession>
    <name>A0A672Z3S9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z3S9"/>
    <dbReference type="Ensembl" id="ENSSORT00005011795.1">
        <property type="protein sequence ID" value="ENSSORP00005011414.1"/>
        <property type="gene ID" value="ENSSORG00005006112.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3S9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
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        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="10">
        <location>
            <begin position="26"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="295" mass="33151" checksum="67A0CD8F029CEC8E" modified="2020-06-17" version="1">FEQYTFITYTFLCFRYSEFLSYGEIMGCLLTAASLFGTCMTCLVAYIFFRYRKTPIVRANNAELSFLLLFSLTLCFLCSLTFIGQPSEWSCMLRHTAFGITFVLCVSCVLGKTIVVLMAFRATLPGSNVMKWFGPVQQRLSVVGFTLIQVLICILWLTISPPFPFKNFKDFKDKIIIECALGSAIGFWAVLGYIGLLALLCFVLAFLARKLPDNFNEAKFITFSMLIFCAVWITFIPAYVSSPGKFSVAVEIFAILASSFGLLICIFIPKCYIILLKPEKNTKKSIMGKGGPRTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3T9</accession>
    <name>A0A672Z3T9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tctn1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the tectonic-like complex (also named B9 complex).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tectonic family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z3T9"/>
    <dbReference type="Ensembl" id="ENSSORT00005011552.1">
        <property type="protein sequence ID" value="ENSSORP00005011177.1"/>
        <property type="gene ID" value="ENSSORG00005006006.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3T9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904491">
        <property type="term" value="P:protein localization to ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040354">
        <property type="entry name" value="TCTN1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011677">
        <property type="entry name" value="TCTN1-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057724">
        <property type="entry name" value="TCTN1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611">
        <property type="entry name" value="TECTONIC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611:SF1">
        <property type="entry name" value="TECTONIC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25752">
        <property type="entry name" value="DUF1619_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07773">
        <property type="entry name" value="TCTN_DUF1619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025519737" evidence="7">
        <location>
            <begin position="23"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3 N-terminal" evidence="9">
        <location>
            <begin position="93"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3" evidence="8">
        <location>
            <begin position="226"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="456" mass="49049" checksum="258643B10A8ADDA0" modified="2020-06-17" version="1">MAAPSAMFRSVLLHIFWILSDAAATENTTSYGFNTTVFADQNDMYNFTDNYTTTENTPFGSTTPTPSSGTAEPPVLSLPVEPLPVSGRLLPSVTDVVILCPCNDHQDVCDANCCCDRQCSEEVALFTGCSVHSVSGSGQLCSRDVASYALRSKVDGYSELQSSVQKETKYDAFCIQSQNRVDGLAHPSPALPTDTNFEWLFQQFSSFVLGSEKDDAQVPAAQPQASSGYQYGDVLERISDSGQREIFSLPASAVTAECVDNNAAAFLMEQSSRCFRSVDLQQDCRRLPPLSVDAYTNIQLLAGKNKDAVVVPVEVGSIVLQSLDGTKIELELSGGEDLRPVLVSPSVCANVVLKVVYAITHNPAGEILSAMLSLVLGFVQEALLPLEQTFHVMFVQEVVPQLAVRSSGNPGYVVGLPLVSGQRTAEYPSDTSILGPHGGGVHGFDSNRRPFCEEFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z467</accession>
    <name>A0A672Z467_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pmt</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine = phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25325"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-methylethanolamine phosphate + S-adenosyl-L-methionine = N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25321"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57781"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58641"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:25322"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>phosphoethanolamine + S-adenosyl-L-methionine = N-methylethanolamine phosphate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20365"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57781"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58190"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:20366"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.103" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z467"/>
    <dbReference type="Ensembl" id="ENSSORT00005011948.1">
        <property type="protein sequence ID" value="ENSSORP00005011564.1"/>
        <property type="gene ID" value="ENSSORG00005006165.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z467"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000234">
        <property type="term" value="F:phosphoethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44307:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE METHYLTRANSFERASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="9">
        <location>
            <begin position="55"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="9">
        <location>
            <begin position="287"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="492" mass="56517" checksum="A1E159D9C3D6B493" modified="2020-06-17" version="1">ADSELRSSMTEFWKEHSKAATVEEMMLDSRAKELTQQELPEILSLLPCLSKCKMLELGAGIGRYTKHLVTRAQHVIAVDFMENFVEKNRQDNGHHSNVTFLQADVTKLEIPENSIDFIFSNWLLMYLSNEELQSFVEKMISWLRPGGFLFFRESCNQRSGDCKRDFNPTCYRTQAQYSHIMTSVQVKDPESSQNFGFDIVLKKQVQTYIEMKNNPNQICWLLEKVLRSSNTNSKFNTFQQFLDNQQYTSRGILRYEKMFGAGYVSTGGPVTTKEFVSLLNLKPGQKVLDVGCGIGGGDFYMAKTFGVEVLGMDLSENMVDIAMERAVSEKLPSVQFEVSDATKRTFSDASFDVIYSRDTILHISDKLALFKRFHSWLKPGGKLLISDYCCGEKPWTPQFEAYVKQRGYILYTPPQYGKFIQEAGFSNVRAEDRTAQFIKVIEDELQRVDAIKDEFIEEFSEEDYLAIVNGWREKLGRSRSGDQRWGLFLATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5H6</accession>
    <name>A0A672Z5H6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 1 regulatory subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ppp1r10</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Component of the PNUTS-PP1 complex (also named PTW/PP1 complex), composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA (or PPP1CB or PPP1CC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030003706.1">
        <property type="nucleotide sequence ID" value="XM_030147846.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030003707.1">
        <property type="nucleotide sequence ID" value="XM_030147847.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030003708.1">
        <property type="nucleotide sequence ID" value="XM_030147848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z5H6"/>
    <dbReference type="FunCoup" id="A0A672Z5H6">
        <property type="interactions" value="765"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012118.1">
        <property type="protein sequence ID" value="ENSSORP00005011732.1"/>
        <property type="gene ID" value="ENSSORG00005006255.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428683"/>
    <dbReference type="CTD" id="5514"/>
    <dbReference type="InParanoid" id="A0A672Z5H6"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="Nitrous oxide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="514"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="592" mass="64402" checksum="EA31D1478305BE1C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZE81</accession>
    <name>A0A672ZE81_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable serine carboxypeptidase CPVL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cpvl</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the digestion of phagocytosed particles in the lysosome, participation in an inflammatory protease cascade, and trimming of peptides for antigen presentation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030014859.1">
        <property type="nucleotide sequence ID" value="XM_030158999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZE81"/>
    <dbReference type="FunCoup" id="A0A672ZE81">
        <property type="interactions" value="435"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015192.1">
        <property type="protein sequence ID" value="ENSSORP00005014763.1"/>
        <property type="gene ID" value="ENSSORG00005007297.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436216"/>
    <dbReference type="CTD" id="54504"/>
    <dbReference type="InParanoid" id="A0A672ZE81"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000096">
        <property type="entry name" value="Carboxypeptidase vitellogenic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF472">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CPVL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025575488" description="Probable serine carboxypeptidase CPVL" evidence="9">
        <location>
            <begin position="23"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="478" mass="53995" checksum="3859E54F7FFF1DBE" modified="2020-06-17" version="1">MWLLRETLSVLLLWALLESAHSRGCSSFFCRKSLRVSGPSGVDPGSPLFVTPYLEKGAIDEARKLSLVGDLPGANVKSYAGYFTVNKKYNSNLFFWFFPALKPDQDKAPVLLWLQGGPGGTSMFGLFVEHGPYVVFKNLTVGLRDFAWTTRYSVLYIDNPVGTGFSFTDDDKGFAQNQDDVGRDLYSALTQFFQMFPEYQANDFYATGESYAGKYVPAVSYYIHKNNPTAKVKINFKGMAIGDGLCDPELMLGGYGEFLYQTGMIDELQKEYVIQQTDIGVKLIQQQKWVEAFQVFDSLLNGDVAPYPSFFQNATGCTNYYNYMTCKEPEDQEYFGPFVTLASVRRAIHVGNLTFHDGSEVEKHLLQDVMKSIKPWLGVLMDNYRVLIYSGQLDVIVAAPLTERFLPTVNWTGASEYKKAPRFHWKVQPSDTEVAGYVRQVGEFFQVIIRGGGHILPYDQPARSFDMLDRFLSTRGWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZEP2</accession>
    <name>A0A672ZEP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome c1, heme protein, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Complex III subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Complex III subunit IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Cytochrome b-c1 complex subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115420123</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="20">Binds 1 heme c group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with FLVCR2; this interaction occurs in the absence of heme and is disrupted upon heme binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029991224.1">
        <property type="nucleotide sequence ID" value="XM_030135364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZEP2"/>
    <dbReference type="FunCoup" id="A0A672ZEP2">
        <property type="interactions" value="1871"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015775.1">
        <property type="protein sequence ID" value="ENSSORP00005015284.1"/>
        <property type="gene ID" value="ENSSORG00005007757.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420123"/>
    <dbReference type="CTD" id="1537"/>
    <dbReference type="InParanoid" id="A0A672ZEP2"/>
    <dbReference type="OrthoDB" id="5925at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000002">
        <property type="entry name" value="Cytochrome c1, heme protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000003">
        <property type="entry name" value="Cytochrome c1, heme protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021157">
        <property type="entry name" value="Cyt_c1_TM_anchor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="CYTOCHROME C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266:SF3">
        <property type="entry name" value="CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81496">
        <property type="entry name" value="Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 20" id="KW-0349">Heme</keyword>
    <keyword evidence="11 20" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" description="covalent" evidence="20">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="20">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="20">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="20">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR602326-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="307" mass="33806" checksum="B4A57C82D2184A35" modified="2020-06-17" version="1">MAALRVVVLSGSGRTLLGTLKAIRTPRASMSFTSLPRNKKVALTTLGVVTAGGAGLALMLHQSVKASDLELHPPHYPWSHAGPLSSLDHASVRRGYQVYKQVCSACHSMEYLAFRNLVGVSHTEAEVKAIAEEVEVVDGPDENGEMFTRPGKLSDYFPKPYPNAEAARVANNGALPPDLSYIVNARHGGEDYVFSLLTGYCEPPAGVTVREGLYYNPYFPGQAIGMAPPIYNEILEFEDGTPATMSQVAKDVCTFLRWAAEPEHDQRKRMGLKMLMGSAILIPLLYYMKRHRWSVLKSRKIAYRPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGH5</accession>
    <name>A0A672ZGH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZGH5"/>
    <dbReference type="Ensembl" id="ENSSORT00005016889.1">
        <property type="protein sequence ID" value="ENSSORP00005016381.1"/>
        <property type="gene ID" value="ENSSORG00005008311.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGH5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="36"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="258" mass="28931" checksum="B73FEBF3E7F7715C" modified="2020-06-17" version="1">CSKGCKSLKHIIQQHRNHIHPSIHSVKSPPDPPRLPSVSVSPSGQIVQGSSVTLTCTSDANPPVTKYTWYKEDEDSPKASGQNFTITDFRSEHSGNYYCEAQNSRGRQNSTSHPIVVSSKWKSVAAGVTVAVLLPIILIFVFLLFRRMRALKTSSEPEDRADNSQQCPPNHRHPEEQDNHHYATVNFSQKKEDPVYSNTGPARCPRHKKEPTFPEYSVLTFHNNSKEQRDRVEETAEDSAVLYSTVNKTKGPRTSAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGV2</accession>
    <name>A0A672ZGV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA-splicing factor ISY1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">isy1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19. Interacts with CPSF3; this interaction is in an RNA independent manner. Interacts with the microprocessor complex subunits DGCR8 and DROSHA; this interaction is in an RNA dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZGV2"/>
    <dbReference type="FunCoup" id="A0A672ZGV2">
        <property type="interactions" value="1961"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016196.1">
        <property type="protein sequence ID" value="ENSSORP00005015702.1"/>
        <property type="gene ID" value="ENSSORG00005007961.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGV2"/>
    <dbReference type="OrthoDB" id="1739576at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="278" mass="31794" checksum="BEF874E1AF4FA630" modified="2020-06-17" version="1">MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECSELPKAEKWRRQIISEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYARVGPKMLDHEGKEVPGNRGYKYFGAARDLPGVRELFEKEPAPAVRKTRAELMKDVDAEYYGYRDEDDGVLLPLEAQYEKQAVLDAVLKWRTDRESRLTTEEEEENIYTVHNEEPDGDDIGEEAEGEEGGVGFIAHVPVPSQKEVEEALVRRKKMELLQRYASETLQAQSQEARTLLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLL2</accession>
    <name>A0A672ZLL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZLL2"/>
    <dbReference type="FunCoup" id="A0A672ZLL2">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018204.1">
        <property type="protein sequence ID" value="ENSSORP00005017684.1"/>
        <property type="gene ID" value="ENSSORG00005008802.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLL2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="49"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="321" mass="36848" checksum="AE597AE17DDF8D20" modified="2020-06-17" version="1">MFFLFVSLRTMGNSTHLTHFILSAYFDTGHLKYLCFVIVLLLYVLMLVSNVLLIVVICVNRSLHEPMYLFLCSLFVNELFGSTGIFPSLLIHILTDIHTVSVSLCFLQIYCLHFYGIVEFLTLAVMSFDRYFAICHPLQYNTHMTRNRISVMVIIPWFYGFLACAVMISLSSSLHLCGNIINKVYCDNYPVVKLACSDTTAVNIYGLIGTFNAIFLPLVFILYTYIRILKVCFSGSKQTRHKAVSTCTPHLASLINFTFGCCFEIISSRFNMNHVPNILRVFLSLYFPTIQPLFNPLMYGLNISNIRMCCRKLKPKWIQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLS6</accession>
    <name>A0A672ZLS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZLS6"/>
    <dbReference type="Ensembl" id="ENSSORT00005018269.1">
        <property type="protein sequence ID" value="ENSSORP00005017749.1"/>
        <property type="gene ID" value="ENSSORG00005008837.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLS6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="32"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="76" mass="8531" checksum="C58557EF3D816277" modified="2020-06-17" version="1">MLVVQTIALAQLVISTRTYGQASLEGEIGRACSVCINEYAQGNKLRRLPCSHEFHIHCIDRWLSDNNTCPICSHPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZNX3</accession>
    <name>A0A672ZNX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZNX3"/>
    <dbReference type="Ensembl" id="ENSSORT00005019066.1">
        <property type="protein sequence ID" value="ENSSORP00005018519.1"/>
        <property type="gene ID" value="ENSSORG00005009140.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZNX3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="4">
        <location>
            <begin position="9"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="73" mass="8354" checksum="A3C632AB8069A4B2" modified="2020-06-17" version="1">MAPTLVEHVVADAGAFLKKSPLQEIGRNIYTLRDVVNEIRDKPTRRSLAVLPYQLNLKEPHPEHIHTGEYTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZST5</accession>
    <name>A0A672ZST5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cugbp, Elav-like family member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115411601</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CELF/BRUNOL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZST5"/>
    <dbReference type="FunCoup" id="A0A672ZST5">
        <property type="interactions" value="1101"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020788.1">
        <property type="protein sequence ID" value="ENSSORP00005020221.1"/>
        <property type="gene ID" value="ENSSORG00005009851.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZST5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12631">
        <property type="entry name" value="RRM1_CELF1_2_Bruno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12634">
        <property type="entry name" value="RRM2_CELF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000013">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000015">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000016">
        <property type="entry name" value="CUGBP Elav-like family member 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034196">
        <property type="entry name" value="CELF1/2_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034198">
        <property type="entry name" value="CELF1/2_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="16"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="102"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="385"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="467" mass="50629" checksum="0696D43649D74F58" modified="2020-06-17" version="1">MNGSLDHPDQPDVDAIKMFVGQIPRSWSEDQLRLFEPFGAVYEINVLRDRSQNPPQSKGCCFITYYTRKSALEAQNALHNMKILPGMHHPIQMKPADTVEDRKLFIGMISKKCNENDIRLMFSPYGQIEECRILRGPDGLSRGCAFVTFTARQMAQSAIKSMHQSQTMEGCSSPIVVKFADTQKDKEQKRMAQQLQQQMQQLSAASMWGNLTGFNSLGPQYLALYLQLLQQSASTGNALNNLHPISGLNAMQNLAALAAAATATQATPTGSSAMTTSSSPLSALTSSGMKYYSTVFQKRLLPHLRSTSSSVNPMTSLGALQSLAAGAGAGMAALNGNLSSGGLSNGSGSTMEYAAAALPSLYNQSLLSQQSISAAGSQKEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVISAKVFIDKHNTSASFVSYDNPVSSQAAIQSMNGFQIGMKRLKVQLKRSKNDSKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTE3</accession>
    <name>A0A672ZTE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">adenylate cyclase</fullName>
            <ecNumber evidence="22">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">adcy2b</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Down-stream signaling cascades mediate changes in gene expression patterns and lead to increased IL6 production. Functions in signaling cascades downstream of the muscarinic acetylcholine receptors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="24">Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with RAF1. Interacts with GNAS. Interacts with the G protein beta and gamma subunit complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22 25">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="22"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZTE3"/>
    <dbReference type="Ensembl" id="ENSSORT00005020227.1">
        <property type="protein sequence ID" value="ENSSORP00005019667.1"/>
        <property type="gene ID" value="ENSSORG00005007158.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZTE3"/>
    <dbReference type="OrthoDB" id="10035433at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000003">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000010">
        <property type="entry name" value="Adenylate cyclase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030672">
        <property type="entry name" value="Adcy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009398">
        <property type="entry name" value="Adcy_conserved_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF6">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06327">
        <property type="entry name" value="Adcy_cons_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039050">
        <property type="entry name" value="Ade_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 22 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 22" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19 22 25" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 22 24" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="16 22 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 22 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 22 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="643"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="694"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="767"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="792"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="837"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="27">
        <location>
            <begin position="307"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="27">
        <location>
            <begin position="920"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="312"/>
            <end position="317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="354"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="972"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="1052"/>
            <end position="1054"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="1059"/>
            <end position="1063"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="1099"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00057708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00062986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRNR" id="PIRNR039050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR039050-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR039050-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1124" mass="126927" checksum="D9C9B860203C6CB7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTK5</accession>
    <name>A0A672ZTK5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cationic amino acid transporter 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">slc7a3a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZTK5"/>
    <dbReference type="FunCoup" id="A0A672ZTK5">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021217.1">
        <property type="protein sequence ID" value="ENSSORP00005020640.1"/>
        <property type="gene ID" value="ENSSORG00005010036.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZTK5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000009">
        <property type="entry name" value="Low affinity cationic amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004755">
        <property type="entry name" value="Cat_AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00906">
        <property type="entry name" value="2A0303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF20">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="586"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="15">
        <location>
            <begin position="561"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="613"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="648" mass="69577" checksum="1F612E9E12FDC678" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUJ6</accession>
    <name>A0A672ZUJ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Enoyl CoA hydratase domain containing 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">echdc2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987858.1">
        <property type="nucleotide sequence ID" value="XM_030131998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZUJ6"/>
    <dbReference type="Ensembl" id="ENSSORT00005020481.1">
        <property type="protein sequence ID" value="ENSSORP00005019918.1"/>
        <property type="gene ID" value="ENSSORG00005009725.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417842"/>
    <dbReference type="CTD" id="55268"/>
    <dbReference type="InParanoid" id="A0A672ZUJ6"/>
    <dbReference type="OrthoDB" id="410701at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000022">
        <property type="entry name" value="methylglutaconyl-CoA hydratase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF44">
        <property type="entry name" value="ENOYL-COA HYDRATASE DOMAIN-CONTAINING PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="34661" checksum="ED35122211F2C1BC" modified="2020-06-17" version="1">MTAVVGRCVSARSGLLGVCLPAGLRLVHRSGARLLAGGGGPVTGPSGAQSRRQHTEVAAGPVEVDLRRLEGEDDGIVEVLMCRHKARNALGHVFVSQMRELVSSLANDSVVRVVVFRSLVPGVFCAGADLKERSVMDNTESDLFVHSLRSLMTQIALLPVPTIAAMDGVALGGGLELALACDLRTAAYSAQMGLIETTRGLLPGAGGSQRLPRMVGITLAKELIFTGRRVGGQTALEIGLVNRAVEQNQTGDAAYREALSLARDILPQAPIAVRMAKEAMNRGVEVDITSAMAIERMCYARVIPTRDRQEGMAAFIEKRPPRYTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZV97</accession>
    <name>A0A672ZV97_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vesicle transport protein SEC20-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bnip1b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC20 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZV97"/>
    <dbReference type="Ensembl" id="ENSSORT00005020903.1">
        <property type="protein sequence ID" value="ENSSORP00005020332.1"/>
        <property type="gene ID" value="ENSSORG00005009912.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZV97"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15865">
        <property type="entry name" value="SNARE_SEC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005606">
        <property type="entry name" value="Sec20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056173">
        <property type="entry name" value="Sec20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825">
        <property type="entry name" value="BNIP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825:SF0">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SEC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03908">
        <property type="entry name" value="Sec20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Sec20 C-terminal" evidence="12">
        <location>
            <begin position="129"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="223" mass="25831" checksum="E21F84E6C281016F" modified="2020-06-17" version="1">MASSMDVHVRICAQEMINMTWKLKDCPGPQSALMDLNSQVKGKFNKLRLRIQDLEQMAREQDRETDRLEIQAETESQRRQMMSNQTAWRKANLACKLAIDNMEKDELLHGGENESTRQRRVTKESLVETSSNITESLMSISRMMSEQVKQSEDAINTLATSSRTVQETNEEFKNMTGTIHLGRKLILKYNRRELTDKLLIFLALALFLATVLYILKKRFLPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZ14</accession>
    <name>A0A672ZZ14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidyl-prolyl cis-trans isomerase D</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">40 kDa peptidyl-prolyl cis-trans isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Cyclophilin-40</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Rotamase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ppid</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Identified in ESR1 or NR3C1/GCR steroid receptor-chaperone complexes. Found in HSP90 chaperone complexes with kinase clients LCK or EIF2AK1. Two monomers associate with one HSP90 homodimer. Interacts with HSP90AA1. Interacts with HSP90AB1; PPID and FKBP4 compete for binding to HSP90AB1 and the interaction is mutually exclusive with the PPID:HSPA8 interaction. Interacts with HSPA8; PPID and STIP1 but not FKBP4 compete for binding to HSPA8 and the interaction is mutually exclusive with the PPID:HSP90AB1 interaction. Interacts with S100A1 and S100A2; the interactions dissociate the PPID:HSP90AA1 interaction. Interacts with S100A6. Interacts with MYB, ILF2, XRCC6, RACK1 and RPS3. Interacts with cytoplasmic dynein 1 intermediate chain (DYNC1I1 or DYNC1I2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="RefSeq" id="XP_030001109.1">
        <property type="nucleotide sequence ID" value="XM_030145249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZZ14"/>
    <dbReference type="FunCoup" id="A0A672ZZ14">
        <property type="interactions" value="1665"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022876.1">
        <property type="protein sequence ID" value="ENSSORP00005022219.1"/>
        <property type="gene ID" value="ENSSORG00005010833.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426953"/>
    <dbReference type="CTD" id="5481"/>
    <dbReference type="InParanoid" id="A0A672ZZ14"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000009">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6 14" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="15">
        <location>
            <begin position="19"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="14">
        <location>
            <begin position="307"/>
            <end position="340"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064827"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074451"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082405"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="370" mass="41107" checksum="232235121CDD4D48" modified="2020-06-17" version="1">MSHPTSSDKPSNAANPRVFFDVDIAGERVGRIVLELFADITPKTAENFRALCTGEKGTGKSTGKPLHFKGCPFHRIIKKFMIQGGDFSNHNGTGGESIYGEKFEDENFHYKHDRVGLLSMANAGPNTNGSQFFITTVPTPHLDGKHVVFGQVLKGMGVVKMLESIDTKEDAPVEPCVIADCGEHKDGDSWGPAPIDGSGDTHPEFPEDSDIDFKDVDKVLAVAEDVKNIGNNLFKGQDWNAAVKKYNKALRYLQVSGEVVEEEQQKKLEPTALSCFLNTAACNLKMQLWQDALESCSEALELNQTNTKALFRRAQAWQGMKEYSKAMVDLKKAQEITPEDKAISNEMKRVQLKIQEEKEKEKKIYAKMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0E9</accession>
    <name>A0A673A0E9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Moesin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115433122</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A0E9"/>
    <dbReference type="FunCoup" id="A0A673A0E9">
        <property type="interactions" value="2668"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023379.1">
        <property type="protein sequence ID" value="ENSSORP00005022714.1"/>
        <property type="gene ID" value="ENSSORG00005007808.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A0E9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17187">
        <property type="entry name" value="FERM_F1_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000003">
        <property type="entry name" value="Radixin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000013">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.450:FF:000001">
        <property type="entry name" value="radixin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.450">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR011259">
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    <dbReference type="InterPro" id="IPR041789">
        <property type="entry name" value="ERM_FERM_C"/>
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        <property type="entry name" value="ERM_helical"/>
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    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
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    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00769">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20492">
        <property type="entry name" value="ERM_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002305">
        <property type="entry name" value="ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
        <property type="entry name" value="Moesin tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025406419" evidence="6">
        <location>
            <begin position="18"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="7">
        <location>
            <begin position="46"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="350"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="399"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="512"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR002305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="620" mass="72829" checksum="982C3607E50BCBD3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3C1</accession>
    <name>A0A673A3C1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein C-ets-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">etsrp</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSSORT00005023806.1">
        <property type="protein sequence ID" value="ENSSORP00005023133.1"/>
        <property type="gene ID" value="ENSSORG00005011207.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A3C1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ets variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF312">
        <property type="entry name" value="ETS VARIANT TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="364" mass="41279" checksum="B741C255A9C7059A" modified="2020-06-17" version="1">MYWDTLIKPTESKDPKIKMELCQTGYYTEDFRTQEVPAGFDFTSYDSKTPGQQQFPAESSYSDTPKASNLYDGKVNTNDTGLFNLDSYQEYNWWSSYPQDGLDQTGYQESLQTYQSVVPQNGQFSPAVEGSHSPFLPAKRMNTAPQSETDQGFSCHSAELYDSDGQRQTASSFWPEYPSSGFNTPLPHPPPAACPQAVEQYCPRVTKRKSTHPQRPEREGPMTAMSAYPGSGPIQLWQFLLELLLDSACRTFISWTGDGWEFKMSDPTEVAKRWGQCKNKPKMNYEKLSRGLRYYYHKNIIHKTAGKRYVYRFVCDIQGMLGKTAQEVLTSLNVLPTNSESWQSTEVPAAALMSDHSSETWASQ</sequence>
</entry>
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    <accession>A0A673A5R8</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="238" mass="25993" checksum="B37A44B0EB17A4BF" modified="2020-06-17" version="1">METAAAYGAAKAGSVAFDPVAFFTHPRTILRLLSWVFSIVVFSCIVNEGYINIGSERLLCVFNNNADACNYGITVGVACFLGSICFLILDVYFPTISNVRDRRRAVLLDLGFSGLASFLWFVGFCFLANQWQATSPDELPLAQGSDAARATIAFCFFSILTWAVLTLSALRRFLAGSHANLFTWQHLDPTPSNGARATPYPIANGATIVTTNPYQAPPFTETLDPQKLTQQRPMAPAF</sequence>
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    <accession>A0A673A8M1</accession>
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        <submittedName>
            <fullName evidence="3">Si:dkey-175g6.2</fullName>
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        <name evidence="3" type="primary">si:dkey-175g6.2</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <sequence length="665" mass="76388" checksum="B9BBDE91D7FA8EA9" modified="2020-06-17" version="1"> </sequence>
</entry>
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            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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        <property type="entry name" value="RNA (RNA) POLYMERASE II ASSOCIATED PROTEIN HOMOLOG"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="299" mass="33438" checksum="0D83DD33D7AFE259" modified="2020-06-17" version="1">MPQKSKAQSWTALKQAGNDRFKTGQYGEATGLYSQAIKELEKSSKKSPEDLGILYSNRAASYLKDGNCAECVKDCNKSLEVYPFNVKSLLRRAAAYEALERYRLAYVDYKTALQIDCNIAAAHDGTNRMTKALTEADGLSWREKLPPIPTVPLSVRQKLTQQTAASQPKQPAPQQNGTKATNPTGPSDKDIKKAKTLKRKEGNAIEKYSQSLQHNPTESLHCLDSRALCYLSLKQYRDAVRDCDEALMIDSSNVKALYRRGQAHRELQVTCVDDLNTLLRVEPKNTAALKLLQEVQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAQ1</accession>
    <name>A0A673AAQ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="18">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">b4galt5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17950"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:31496"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Golgi apparatus membrane</location>
            <topology evidence="18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A673AAQ1"/>
    <dbReference type="FunCoup" id="A0A673AAQ1">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027026.1">
        <property type="protein sequence ID" value="ENSSORP00005026254.1"/>
        <property type="gene ID" value="ENSSORG00005012557.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AAQ1"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008489">
        <property type="term" value="F:UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009101">
        <property type="term" value="P:glycoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000037">
        <property type="entry name" value="Beta-1,4-galactosyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF45">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="107"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="247"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="382" mass="44537" checksum="3E54A269C3455378" modified="2020-06-17" version="1">IMPTHLRFRRRSFLGLLFLFSLSTSALYFIYSAPGIVNEYVFMVQARGIQIRENVRNIGVQVLEQVVRGTYSINGTDYSYDFNMSESDAPPTTYLPEGFTYLPSQVCPEKLPSMKGRLEVNMSEVSLEEVERSLLQKEPNVELGGYWKPKDCLPRWKVAILVPFRNRHEHLPILLRHLVPVLQKQRLQFAFYVIEQGGNEPFNRAMLFNVGYKEAMKDLDWDCLIFHDVDHLMENDRNYYGCTDMPRHFAVKLDKYSYMLPYNEFFGGVSGLTVKQLKKINGFPNAFWGWGGEDDDLWNRVQYANYTVSRPHGEQGRYKSIPHHHRGEVQFLGRYSLLRHSKERQKVDGLNNLNYSPLVSRRPLYTNITVNLSRKLAPIADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AC26</accession>
    <name>A0A673AC26_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Cyclic nucleotide gated channel subunit alpha 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AC26"/>
    <dbReference type="FunCoup" id="A0A673AC26">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027589.1">
        <property type="protein sequence ID" value="ENSSORP00005026806.1"/>
        <property type="gene ID" value="ENSSORG00005012847.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AC26"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000002">
        <property type="entry name" value="Cyclic nucleotide gated channel alpha 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000030">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.300:FF:000002">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032406">
        <property type="entry name" value="CLZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF3">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED OLFACTORY CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16526">
        <property type="entry name" value="CLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="3" id="KW-0140">cGMP</keyword>
    <keyword evidence="10" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0552">Olfaction</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="459"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="650"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="600"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="674" mass="77666" checksum="407784E9AB4F7D89" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEW1</accession>
    <name>A0A673AEW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673AEW1"/>
    <dbReference type="FunCoup" id="A0A673AEW1">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027721.1">
        <property type="protein sequence ID" value="ENSSORP00005026937.1"/>
        <property type="gene ID" value="ENSSORG00005012898.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AEW1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012911">
        <property type="entry name" value="PP2C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07830">
        <property type="entry name" value="PP2C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 18" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="20">
        <location>
            <begin position="77"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="381" mass="41876" checksum="43200A92FF2EBCC1" modified="2020-06-17" version="1">MRTARKGSVEMPAFVRQLVKETEKRVSSFFKGGRGGGAAEGEHPAEGEKEEVIPSPYLDRPVLDKLTEEGCTRWGLTYALGSMQGWRANMEDFHNCVPQLGGELADWSFFAVFDGHAGSTVAQHCSQHLLGHILATGGIRPEDDPEKVKGAIIEGFLQTDKHLHTVARREGWERGGTTVVATLISPYYIYFANCGDSRAMLCRSGQVCFSTDDHKPYSPLEKERIESAGGSVSLQRINGSLAVSRALGDFSYKGAENRSPSQQMVSPEPEVCVIERSPADEFLVLACDGVWDTITNEELCAFIHNRLQVCTDLRDVCTQVIDLCLYKGSLDNISIILLCFPGAPQLSAEALHQEAELEDLLESKVAGVWHLLWAIISVYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AH55</accession>
    <name>A0A673AH55_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Macrophage migration inhibitory factor</fullName>
            <ecNumber evidence="9">5.3.2.1</ecNumber>
            <ecNumber evidence="8">5.3.3.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">L-dopachrome isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">L-dopachrome tautomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Phenylpyruvate tautomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mif</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-phenylpyruvate = enol-phenylpyruvate</text>
            <dbReference type="Rhea" id="RHEA:17097"/>
            <dbReference type="ChEBI" id="CHEBI:16815"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="EC" id="5.3.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-dopachrome = 5,6-dihydroxyindole-2-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13041"/>
            <dbReference type="ChEBI" id="CHEBI:16875"/>
            <dbReference type="ChEBI" id="CHEBI:57509"/>
            <dbReference type="EC" id="5.3.3.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.2.1" evidence="9"/>
    <dbReference type="EC" id="5.3.3.12" evidence="8"/>
    <dbReference type="RefSeq" id="XP_030001211.1">
        <property type="nucleotide sequence ID" value="XM_030145351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AH55"/>
    <dbReference type="FunCoup" id="A0A673AH55">
        <property type="interactions" value="613"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029417.1">
        <property type="protein sequence ID" value="ENSSORP00005028604.1"/>
        <property type="gene ID" value="ENSSORG00005013686.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427013"/>
    <dbReference type="CTD" id="4282"/>
    <dbReference type="InParanoid" id="A0A673AH55"/>
    <dbReference type="OrthoDB" id="255819at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004167">
        <property type="term" value="F:dopachrome isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050178">
        <property type="term" value="F:phenylpyruvate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="entry name" value="Macrophage Migration Inhibitory Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001398">
        <property type="entry name" value="Macrophage_inhib_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019829">
        <property type="entry name" value="Macrophage_inhib_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954">
        <property type="entry name" value="D-DOPACHROME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11954:SF6">
        <property type="entry name" value="MACROPHAGE MIGRATION INHIBITORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01187">
        <property type="entry name" value="MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="Tautomerase/MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01158">
        <property type="entry name" value="MIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="115" mass="12355" checksum="C970E16E14302C42" modified="2020-06-17" version="1">MPMFVVNTNVAKSEVPAALLSEATEELAKAMGKPAQYIAVHINPDQMMMFGGKGDPCALCSLHSIGKISGAHNKQYSKLLCGLLNKHLGISADRIYVNFVDMDAANVAWNNTTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJ99</accession>
    <name>A0A673AJ99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AJ99"/>
    <dbReference type="Ensembl" id="ENSSORT00005029191.1">
        <property type="protein sequence ID" value="ENSSORP00005028382.1"/>
        <property type="gene ID" value="ENSSORG00005013487.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJ99"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000001">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ZP" evidence="16">
        <location>
            <begin position="117"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="442" mass="49980" checksum="7FE8D640A597DF66" modified="2020-06-17" version="1">MHGVSKTFFYRRIHILIVKENIFFRNSWLINSKYVSATMRMKWTALCLVALLLYGSFCDAQRFQEIIGQSPHDHQQGKQIIERPLQWSFPADPTTRPEILIPNEIRHPASANSVAVNCGENIVQVIVQKDMFGIGQYVDPRHLTLGSCGFVTEDIANDGSSVIVFQSEVHGCDSSLSMTQDSLIYTYDLKYNPEGVVMRINRAAVTVECHYPRIHNVSSLPLIPYWVPFSATKVSEEFLYFTLKLMTDDWQHQRSTDVYYLGTSIKIEASVMQYFHVPLRVFIDRCVISIRPDPTSNPNYAFIENGCLVDGINSASKFMNRDADDKLRFELAALMFQGYENERLYITCYLKATSINHGINSERRACSYSNGWYEASGVNEACNTCDSSGPASSGQSTFVNYNPGSTSGSAGSGQRLWNGRARSPWKFAFIKTQMLNSTLPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKK3</accession>
    <name>A0A673AKK3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prostacyclin synthase</fullName>
            <ecNumber evidence="7">4.2.1.152</ecNumber>
            <ecNumber evidence="6">5.3.99.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Hydroperoxy icosatetraenoate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Prostaglandin I2 synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">ptgis</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a hydroperoxyeicosatetraenoate = an oxoeicosatetraenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:55556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59720"/>
            <dbReference type="ChEBI" id="CHEBI:131859"/>
            <dbReference type="EC" id="4.2.1.152"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:55557"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>prostaglandin H2 = prostaglandin I2</text>
            <dbReference type="Rhea" id="RHEA:23580"/>
            <dbReference type="ChEBI" id="CHEBI:57403"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="EC" id="5.3.99.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:23581"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 27 28">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 27">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.152" evidence="7"/>
    <dbReference type="EC" id="5.3.99.4" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673AKK3"/>
    <dbReference type="FunCoup" id="A0A673AKK3">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029695.1">
        <property type="protein sequence ID" value="ENSSORP00005028877.1"/>
        <property type="gene ID" value="ENSSORG00005013788.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AKK3"/>
    <dbReference type="OrthoDB" id="6692864at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106256">
        <property type="term" value="F:hydroperoxy icosatetraenoate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008116">
        <property type="term" value="F:prostaglandin-I synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024204">
        <property type="entry name" value="Cyt_P450_CYP7A1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24306">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24306:SF4">
        <property type="entry name" value="PROSTACYCLIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000047">
        <property type="entry name" value="Cytochrome_CYPVIIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 27" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12 27 28" id="KW-0349">Heme</keyword>
    <keyword evidence="18 27 28" id="KW-0408">Iron</keyword>
    <keyword evidence="22" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="23" id="KW-0456">Lyase</keyword>
    <keyword evidence="20 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 27 28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="9" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="30" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025564809" description="Prostacyclin synthase" evidence="30">
        <location>
            <begin position="24"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="28">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00017409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00033404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00045141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRNR" id="PIRNR000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="489" mass="56697" checksum="87FF5CEF149A7B06" modified="2020-06-17" version="1">MLWTIFLLLNGLFVLVLIWSSRSRQKNEPPLDKGPIPWLGHALTFRKDAAKFLAKMKTKHGDIFTLRVAGKYVTVLLDPDSYDAVLYDSVCLDNSQCKNQLLKRIFSLQLPNIKPTTERRWMEQHFQGLSLSRLSISMNTNLQNLLLNDWKGCSPTEWREEGLFSFCYSLLFRAGYLTFFERRNDATAVYKEFRKFDDLLAKLARCSLKRDERRTVMSTQERLWELLSSNWSSEESESNSWQQSYRQFLQEEGVDAEMQNRALLLQLWTTQCNAGPASFWLLGFLLTHPKAMEAVKSEIRGLTLQDSSLQHAPINPLEGLSTPVFDSVLSETLRLSAAVLISREVVQDKILRMANGQEYHLRRGDRVCLFPFLSPQMDQDIHEEPQMFKYDRFLNEDKTEKKIFYKDGKRLKHYTLPWGAGGNVCVGKEFAVTAIKQFVFLVLTHLDLEMCDREAKLPPVNPSRYGFGIVQPDGDLKVRYRLKKTQHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALG8</accession>
    <name>A0A673ALG8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="8">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">uck2b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673ALG8"/>
    <dbReference type="FunCoup" id="A0A673ALG8">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031041.1">
        <property type="protein sequence ID" value="ENSSORP00005030196.1"/>
        <property type="gene ID" value="ENSSORG00005014408.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALG8"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="10">
        <location>
            <begin position="29"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="267" mass="30369" checksum="157801385B05BA4E" modified="2025-10-08" version="2">MHIASMAGDSETHIRDRGENNSVIRQPFLIGVSGGTASGKSSVCEKIMELLGQNKIDHHQRQVAILSQDSFYKVLTPEQKAKALKGQFNFDHPDAFDNELIMQTLRQILQGKTVQIPVYDFVTHSRKDEFITVYPADVVLFEGILMFYSQEIRDLFQMKLFVDTDPDTRLSRRVLRDISERGRELEQVLAQYITFVKPAFEEFCLPTKKYADVIIPRGADNLVAINLIVQHIQDILNGGLSKRHNGCTNGHSTPRQRRTSESSSRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APS3</accession>
    <name>A0A673APS3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Chemokine (C-C motif) receptor 9a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673APS3"/>
    <dbReference type="Ensembl" id="ENSSORT00005031261.1">
        <property type="protein sequence ID" value="ENSSORP00005030407.1"/>
        <property type="gene ID" value="ENSSORG00005014497.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APS3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF664">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="293"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="323" mass="34949" checksum="7EC606C4975FE590" modified="2020-06-17" version="1">YGPGESTDVYTFTDHVTQSCGSSGTSTSRPVLGNHAGGGVGNLAVVWIYLHFQRRLKTMTDVYLLNLAVADLLFLVTLPLWAAEALHGWNFGSTLCKLNSALYKVNLFSSMLLLTCISVDRYVVIVQTTKAQNTRAERRRCSRLVCLALIFATTTPTEEFCTAAWVPSSRGNLTKIATLSLQVTMGFCLPFIIMAFCYSHRGHAAQNAQLPEAKAWCHPGGGGGVRISQLPYNAVLVVEAAQASNMTLTDCEQLRRFDITGQVLKSLPTLTPVSTLPLRLRGVRFPVIPLAAKSPSGKSSRSPLSSARTSVMSDCDTSQALSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQQ1</accession>
    <name>A0A673AQQ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ankyrin repeat and SOCS box containing 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">asb4</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AQQ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005032880.1">
        <property type="protein sequence ID" value="ENSSORP00005031995.1"/>
        <property type="gene ID" value="ENSSORG00005015194.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQQ1"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03723">
        <property type="entry name" value="SOCS_ASB4_ASB18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="143"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="255"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="383"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="48348" checksum="3F5C1E33EA08E447" modified="2020-06-17" version="1">MFPSYISTCPLSPRKQAAEQLKKRFLEALQANDTQEVLEILHTRKIDIDAVLEVDDPSMVLASYKQGYWLPGYKLEKSWAMALHVCVMFNAVETALVLLQEGASLNRMPNGKTPLHVACEVSNSDCVALLLAHGAKVNSLSLSGHSPLHYCITTESVDCARQLILKGAHVDMHSHNNDEVTPLHTAARFGIPELVALYLIHGASVDALNSVQETPLMTAAFWAFDSKEQTYSQDHHFVCRLLLDNKADPNLQEEDHKTALHKAAWNCDHILMQMLLEAGADARAMDINGCAPIQYLLKVTDVRPMAIPEICYQLLLNYNAARIYPPQFHKVLQSCYDYPRVVEILVNSYERLKPTKKWRSAIPDDCYKRHQTFYDSLFAVCTNTPRSLLHLVRCAVRSAMGGFSQRGIAELPLPTSMKKYILLEPEGILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQY8</accession>
    <name>A0A673AQY8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 15">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ltn1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the LTN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 15"/>
    <dbReference type="FunCoup" id="A0A673AQY8">
        <property type="interactions" value="1697"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031679.1">
        <property type="protein sequence ID" value="ENSSORP00005030817.1"/>
        <property type="gene ID" value="ENSSORG00005014644.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQY8"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001251">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054477">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056241">
        <property type="entry name" value="LTN1_HEAT_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054476">
        <property type="entry name" value="Ltn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054478">
        <property type="entry name" value="LTN1_UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22958">
        <property type="entry name" value="Ltn1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24618">
        <property type="entry name" value="LTN1_E3_ligase_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22999">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23009">
        <property type="entry name" value="UBC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1711"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1762" mass="197702" checksum="12FC74E60F387FED" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARI4</accession>
    <name>A0A673ARI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rbm33a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ARI4"/>
    <dbReference type="FunCoup" id="A0A673ARI4">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031920.1">
        <property type="protein sequence ID" value="ENSSORP00005031053.1"/>
        <property type="gene ID" value="ENSSORG00005014812.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARI4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039878">
        <property type="entry name" value="RBM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22014">
        <property type="entry name" value="RNA-BINDING PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22014:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="1167"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="650"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="775"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="875"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="661"/>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="892"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="930"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="982"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="997"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1238" mass="138209" checksum="6946634AEC825871" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASC5</accession>
    <name>A0A673ASC5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Retinol dehydrogenase 14-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rdh14b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ASC5"/>
    <dbReference type="Ensembl" id="ENSSORT00005033464.1">
        <property type="protein sequence ID" value="ENSSORP00005032570.1"/>
        <property type="gene ID" value="ENSSORG00005015458.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASC5"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF72">
        <property type="entry name" value="RETINOL DEHYDROGENASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="324" mass="35515" checksum="3FC3B4A176696541" modified="2020-06-17" version="1">MSTAVLVAAVVGGGVLFLMRRLFPQQKKVVKLLQYSADTMRGKTVIVTGANSGIGKALAGELLKLQARVIMACRDQGSAEQAAQDIKKQAGPEQGEVVIKHLDLASLRSVRKFCEEINAEESKIDILVNNAGIFRCPYTKTEDGFEMQLGVNHLGHFLLTHLLLDLLKRSTPSRIVVVSSKLYKSGHINFDDLNSEVNYNKSFCYSQSKLANLLFTLELARQLEGTGVTVNALTPGIVRTRLGRHVQIPLLAKPLFYLASLVFFRSPLEGAQTPLYLACSPEVEGVSGKCFADCEEEELMAKATDDQAAKKLWDISSRMVGLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATM9</accession>
    <name>A0A673ATM9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115412848</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029981364.1">
        <property type="nucleotide sequence ID" value="XM_030125504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ATM9"/>
    <dbReference type="FunCoup" id="A0A673ATM9">
        <property type="interactions" value="426"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033924.1">
        <property type="protein sequence ID" value="ENSSORP00005033020.1"/>
        <property type="gene ID" value="ENSSORG00005015657.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412848"/>
    <dbReference type="InParanoid" id="A0A673ATM9"/>
    <dbReference type="OrthoDB" id="6147321at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF847">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="311" mass="35606" checksum="0FA93E47F46DC16E" modified="2020-06-17" version="1">MENYTNTFHFNLTMFVNIGSYRYPAFVLCLLMFAFIVSANIIILLVISQERTLHEPMYMFIALLSVNALYGSTGFFPRFLMDLLSDTHLISRPACFTQIYIIYTYASYEMTILGIMAFDRYVAVCEPLHYHNKMTHKKVCILSAFAWIMPAFSVATCLYLAIRLPLCGNKILKVYCAVWNVAKLSCVSTLISNIVGLLVAVVTVFLPLIYVLYTYCRIIIVCSKMSSEFKSKVFQTCLPHTISFVTFSIAVFCDVALSRIDVEKLNPYLAVILSLEFVLIPPVLNPLVYGLTLPEIRKHILRMWPWFCKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AW52</accession>
    <name>A0A673AW52_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vascular endothelial growth factor A-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">vegfaa</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AW52"/>
    <dbReference type="Ensembl" id="ENSSORT00005034458.1">
        <property type="protein sequence ID" value="ENSSORP00005033549.1"/>
        <property type="gene ID" value="ENSSORG00005015890.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AW52"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005172">
        <property type="term" value="F:vascular endothelial growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050930">
        <property type="term" value="P:induction of positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060754">
        <property type="term" value="P:positive regulation of mast cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038084">
        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023581">
        <property type="entry name" value="PD_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050507">
        <property type="entry name" value="PDGF/VEGF_growth_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025:SF5">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR A, LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00249">
        <property type="entry name" value="PDGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 3" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025641569" evidence="4">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="5">
        <location>
            <begin position="36"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="138" mass="15645" checksum="1550C03B08A70B1A" modified="2020-06-17" version="1">MNFVVSLVQILLAAVFHLSTVKTASINKGMDKSKNEVIPLLEVITKSQCQPRDMLVDIFQEYPEDTEHMYIPSCVVLKRCGGCCTDEAFECVPTETRNVTLQVMRVRPKVSQHTIDLRFTEHQQCECRSKPGVSAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AXX2</accession>
    <name>A0A673AXX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DnaJ homolog subfamily C member 5B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115437232</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with the chaperone complex consisting of HSC70 and SGTA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AXX2"/>
    <dbReference type="FunCoup" id="A0A673AXX2">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035410.1">
        <property type="protein sequence ID" value="ENSSORP00005034490.1"/>
        <property type="gene ID" value="ENSSORG00005016278.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AXX2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000017">
        <property type="entry name" value="dnaJ homolog subfamily C member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051434">
        <property type="entry name" value="DnaJ_C_subfamily_member5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027:SF6">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="20"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="201" mass="22428" checksum="9E5EF3A95B366A06" modified="2020-06-17" version="1">CVCNLIGAWRQRTLSTSGESLYQVLGLEKGCSHEDIKKSYRKLALRFHPDKNPDNPEAAEKFKELNSAHAVLSDNTKRNIYDSYGSLGLYVAQQFGEDNVNTYFMLSTWWAKGLFAVCGILTGCYCCCCLCCCCNCCCGKLKPTPTADGAEQEYVSPDDLEEEIRNDGDNNDIPIMQQPTNASEKTQLIGDGHRRYGDTYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYR1</accession>
    <name>A0A673AYR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylation factor-like protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115412183</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AYR1"/>
    <dbReference type="Ensembl" id="ENSSORT00005034420.1">
        <property type="protein sequence ID" value="ENSSORP00005033512.1"/>
        <property type="gene ID" value="ENSSORG00005015864.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AYR1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04157">
        <property type="entry name" value="Arl6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000457">
        <property type="entry name" value="ADP-ribosylation factor-like protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041839">
        <property type="entry name" value="Arl6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 16 18" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="222" mass="25552" checksum="5296919B463729A8" modified="2020-06-17" version="1">MGLLDKLSGWLGLRKKEVNVLCLGLDNSGKTTIINQLKPANTQAQDIVPTIGFNIEKFKSSSLSFTVFDMSGQSRYRNLWEHYYKESHAIIFVIDSGDKLRMVVAKEELDTLLNHEDIRSKKIPVLFFANKMDLRDAMSSVKVSQLLCLENIKDKPWHICASDAIKGEGLQEGLDWLQGRIKHSKYQYHQFISFKCVCEYCQHTIEMCFFCIHRTNCTVSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZA3</accession>
    <name>A0A673AZA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane and ubiquitin-like domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tmub1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AZA3"/>
    <dbReference type="FunCoup" id="A0A673AZA3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035373.1">
        <property type="protein sequence ID" value="ENSSORP00005034454.1"/>
        <property type="gene ID" value="ENSSORG00005016253.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040352">
        <property type="entry name" value="TMUB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557">
        <property type="entry name" value="PROTEIN C7ORF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="12">
        <location>
            <begin position="97"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="56"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="240" mass="26251" checksum="46C845CF57759356" modified="2020-06-17" version="1">MALIEGVGDEVTLLFGSLLLLVVLLLAWVSTRTAEPPEHLFTTAPAPSDRLLTEALPTASEDRRVEKEGEGEGEGGGRGDGLRSRGGGEGAESQRDMVVRLKFLNDTERTAQVKPQDTVGYIKRTYFAGQEQQVRLIYQGQLLQDDAQTLSSLNLVHNCVLHCHISQQASRGAPTGPRPQDQVQVALNVGSLMVPLLVLMLSVLWYCQIQYRQFFTAPATASLVGVTIFLSLVAFGVYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0U0</accession>
    <name>A0A673B0U0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ttc36</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030007187.1">
        <property type="nucleotide sequence ID" value="XM_030151327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B0U0"/>
    <dbReference type="FunCoup" id="A0A673B0U0">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035928.1">
        <property type="protein sequence ID" value="ENSSORP00005034999.1"/>
        <property type="gene ID" value="ENSSORG00005016502.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431076"/>
    <dbReference type="CTD" id="143941"/>
    <dbReference type="InParanoid" id="A0A673B0U0"/>
    <dbReference type="OrthoDB" id="539634at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006570">
        <property type="term" value="P:tyrosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000213">
        <property type="entry name" value="Tetratricopeptide repeat domain 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038906">
        <property type="entry name" value="TTC36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405">
        <property type="entry name" value="CDNA SEQUENCE BC021608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="202" mass="21969" checksum="E97E6E347074DCB2" modified="2020-06-17" version="1">MPSFFLSLVSLHTGKMASVHDRAVLQAIFNPTTPFGDIPGLNQEEELIDDDSGFDTELLKQVKELEMRGVSAAEAGDLQAALQLFSQAIQILPQRASAYNNRAQALRLQGNTAGALQDLNEAISLSGGCGRTGCQALVQRGLLRRLVCQNDEARADFEKAAALGNEFARQQAVVLNPYAALCNRMLSEVINKLRNPEVPEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5U3</accession>
    <name>A0A673B5U3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">map3k10</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="RefSeq" id="XP_030007572.1">
        <property type="nucleotide sequence ID" value="XM_030151712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B5U3"/>
    <dbReference type="FunCoup" id="A0A673B5U3">
        <property type="interactions" value="950"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037675.1">
        <property type="protein sequence ID" value="ENSSORP00005036709.1"/>
        <property type="gene ID" value="ENSSORG00005017289.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431396"/>
    <dbReference type="CTD" id="4294"/>
    <dbReference type="InParanoid" id="A0A673B5U3"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12059">
        <property type="entry name" value="SH3_MLK1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000079">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035779">
        <property type="entry name" value="MLK1-3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016231">
        <property type="entry name" value="MLK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF39">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000556">
        <property type="entry name" value="MAPKKK9_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="103"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="197"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="51"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="586"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="632"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="810"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="938"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="476"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="653"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="831"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1049"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="203"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000556-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000556-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1113" mass="121026" checksum="71BA88B7984605A3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B8V5</accession>
    <name>A0A673B8V5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pelp1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RIX1/PELP1 family.</text>
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    <dbReference type="FunCoup" id="A0A673B8V5">
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    <dbReference type="Ensembl" id="ENSSORT00005037856.1">
        <property type="protein sequence ID" value="ENSSORP00005036888.1"/>
        <property type="gene ID" value="ENSSORG00005017352.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B8V5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012980">
        <property type="entry name" value="PELP1_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012583">
        <property type="entry name" value="RIX1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105:SF1">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08166">
        <property type="entry name" value="PELP1_HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08167">
        <property type="entry name" value="RIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein RIX1 N-terminal" evidence="9">
        <location>
            <begin position="37"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="PELP1 middle" evidence="8">
        <location>
            <begin position="408"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="PELP1 middle" evidence="8">
        <location>
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</entry>
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    <accession>A0A673BIR0</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <sequence length="676" mass="76101" checksum="6C25272F4C515543" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQN7</accession>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Kurtiformes</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <dbReference type="Ensembl" id="ENSSORP00005043514.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12830" checksum="E90D77797B98456F" modified="2020-06-17" version="1">EDITYHYPQDSVTNNIFSPVCESRAASPHVRDSAEPNIPAPSKYSGNPDTCRDFFTQLQLIFDSQPVRFAKDSVKIAYVASLLEGPPLSYFNALFEQDSPAVQSFSALIMRLRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Euteleostomi</taxon>
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            <taxon>Neoteleostei</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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            <location evidence="2">Focal adhesion</location>
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        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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        <property type="entry name" value="Vinculin-bd_dom"/>
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        <property type="entry name" value="TALIN-2"/>
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    <dbReference type="Pfam" id="PF25177">
        <property type="entry name" value="Talin_VBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21865">
        <property type="entry name" value="TLN1-like_RS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08913">
        <property type="entry name" value="VBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109880">
        <property type="entry name" value="A middle domain of Talin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="11">
        <location>
            <begin position="88"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="12">
        <location>
            <begin position="2294"/>
            <end position="2533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="906"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1052"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="2501"/>
            <end position="2528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2542" mass="271721" checksum="CAA6CFC5E9BD20B0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSS1</accession>
    <name>A0A673BSS1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BSS1"/>
    <dbReference type="Ensembl" id="ENSSORT00005047016.1">
        <property type="protein sequence ID" value="ENSSORP00005045861.1"/>
        <property type="gene ID" value="ENSSORG00005021043.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BSS1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="71" mass="8285" checksum="B757CA63D5D0A67B" modified="2020-06-17" version="1">MWDCTCENCILIIERQRVSWRRRWRCAGSRPTRAWRASSRSPSEYSAPGSAYPGPRRGQTREPRRGATSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUD3</accession>
    <name>A0A673BUD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1</fullName>
            <ecNumber evidence="10">2.1.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">TAR RNA-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tarbp1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in a subset of tRNA. Selectively mediates Gm18 methylation of tRNAGln-TTG/CTG and tRNASer-TGA/GCT. Gm18 modification can enhance the stability of modified tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(18) in tRNA + S-adenosyl-L-methionine = 2'-O-methylguanosine(18) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20077"/>
            <dbReference type="Rhea" id="RHEA-COMP:10190"/>
            <dbReference type="Rhea" id="RHEA-COMP:10192"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="EC" id="2.1.1.34"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:20078"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer and homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.34" evidence="10"/>
    <dbReference type="Ensembl" id="ENSSORT00005044900.1">
        <property type="protein sequence ID" value="ENSSORP00005043788.1"/>
        <property type="gene ID" value="ENSSORG00005020200.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUD3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141100">
        <property type="term" value="F:tRNA (guanine(18)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18091">
        <property type="entry name" value="SpoU-like_TRM3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000010">
        <property type="entry name" value="probable methyltransferase TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025806">
        <property type="entry name" value="TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056921">
        <property type="entry name" value="TARBP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045330">
        <property type="entry name" value="TRM3/TARBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044748">
        <property type="entry name" value="Trm3/TARBP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029:SF11">
        <property type="entry name" value="METHYLTRANSFERASE TARBP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12029">
        <property type="entry name" value="RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25050">
        <property type="entry name" value="TARBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51624">
        <property type="entry name" value="SAM_MT_TRMH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TARBP1" evidence="14">
        <location>
            <begin position="271"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="13">
        <location>
            <begin position="1409"/>
            <end position="1550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1570" mass="177037" checksum="D48AC5FC5DA4A5B3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUN2</accession>
    <name>A0A673BUN2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferroptosis suppressor protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Apoptosis-inducing factor homologous mitochondrion-associated inducer of death</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">p53-responsive gene 3 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">aifm2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Putative FAD-dependent oxidoreductase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>menadione + NADH + H(+) = menadiol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:69695"/>
            <dbReference type="ChEBI" id="CHEBI:6746"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28869"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:69696"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>menaquinone-4 + NADH + H(+) = menaquinol-4 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:74079"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78277"/>
            <dbReference type="ChEBI" id="CHEBI:193091"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:74080"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>phylloquinone + NADH + H(+) = phylloquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:74075"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18067"/>
            <dbReference type="ChEBI" id="CHEBI:28433"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:74076"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ubiquinone-10 + NADH + H(+) = ubiquinol-10 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:61984"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46245"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:61985"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>6-hydroxy-FAD</name>
            <dbReference type="ChEBI" id="CHEBI:60470"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030012769.1">
        <property type="nucleotide sequence ID" value="XM_030156909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BUN2"/>
    <dbReference type="FunCoup" id="A0A673BUN2">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045967.1">
        <property type="protein sequence ID" value="ENSSORP00005044834.1"/>
        <property type="gene ID" value="ENSSORG00005020616.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434775"/>
    <dbReference type="CTD" id="84883"/>
    <dbReference type="InParanoid" id="A0A673BUN2"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008637">
        <property type="term" value="P:apoptotic mitochondrial changes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000006">
        <property type="entry name" value="apoptosis-inducing factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="14">
        <location>
            <begin position="15"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="375" mass="40335" checksum="F8FC0DA669C43D03" modified="2020-06-17" version="1">MGGQSSSIDRFQGVRVVVVGGGFGGIAAAQGLKSEGIPFTLIDMRDAFHHNVASLRASVQPGFAQSTFIPFAETFKDSFVQGRVEQVDVDRQTVILRDGQEIQYTHLILCTGTDGPFPGKFNTEASFQTAIQAYDDMVTQMQAADSVLVIGGGSTGVEMAAEIKTEYPQKKVVLVHSRIALADSELLPSVRQQAKEVLLEKGVELLLGQKVTNLSQLQLNVTRKNTEVTTDKGLTLTTDLIICCTGVRVNSAAYASNLADCLADDGALKVNEHLQVQGFSNIFAVGDCANIAEPKMAYHAGLHAAVAVSNISNSLSGKPLTSYRTGSVTMLLAMGRDDGVGQFNGLRLPRCLVALGKSRDLLLWKSWREMEQKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BX84</accession>
    <name>A0A673BX84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein 121-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115417361</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM121 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BX84"/>
    <dbReference type="Ensembl" id="ENSSORT00005048726.1">
        <property type="protein sequence ID" value="ENSSORP00005047546.1"/>
        <property type="gene ID" value="ENSSORG00005021745.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BX84"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032776">
        <property type="entry name" value="CECR6/TMEM121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042314">
        <property type="entry name" value="TMEM121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31046">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31046:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14997">
        <property type="entry name" value="CECR6_TMEM121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="39792" checksum="7BA51922FDFB99C2" modified="2025-10-08" version="2">MVMVPPTSTTANKPHICLLSVLIMTSLALMDAYLVEQNPGPRRLSVCITVLAGDACFLVVLRYVGVWVGLEVHTARRGYAMILWFFYIFILEIKVYFVYQNYKSENESAGVNRRVLTLLLSVFTPIVFLTLAAVDHLEYLRPYKKREEIRSRLFWVVVDLLDVLDVQANLWELQRDGRPLWVEGLTFFYCYILLLVLPCVSLSEISLQGVNIVPHKMMLYPLVSLASINITTLLIRGGNLLIFHDLRVSGVMMAKNIIAIVVKSCSLVQYRKHQPETPPPGKDEEQKKDAPTTVAAVQTVLVPRVVIEDFTSIPEEEEEEEEQEEEKEEEEEEEEEEEEEDSRENQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXL8</accession>
    <name>A0A673BXL8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BXL8"/>
    <dbReference type="Ensembl" id="ENSSORT00005046527.1">
        <property type="protein sequence ID" value="ENSSORP00005045377.1"/>
        <property type="gene ID" value="ENSSORG00005020845.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BXL8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025693972" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="14"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="126" mass="14548" checksum="1A670EE96A1D630A" modified="2020-06-17" version="1">VLIHLFLCMFCSSPECKGEDKVTQPTGDVTAVQGLTLTLQCTFETSDTNAYLYWYKQEVNDYPRYMLHRLRSGSETYKAPEFQDERFDTEINQTSFPLKIQKLHVSDSAVYYCALQPLKGQITMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYZ0</accession>
    <name>A0A673BYZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BYZ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005049396.1">
        <property type="protein sequence ID" value="ENSSORP00005048206.1"/>
        <property type="gene ID" value="ENSSORG00005022009.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BYZ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025654541" description="Receptor ligand binding region domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="6">
        <location>
            <begin position="5"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="164" mass="18500" checksum="24D3C711D512E04C" modified="2020-06-17" version="1">MTAGILALVSSIGCMSAGSLQTLADAMHIPHLFIQRSPAGTPRSACPPTSRSPGTDDYTLMVRPPVYLNEVIMQVVSEYSWQKFILFYDSDFDIRGIEDFLDRTSQQGMDVSLQKVESNINMMITSLFRTLRVEELHRYRDTLRRAVLFMSPATAKAFITEVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZ25</accession>
    <name>A0A673BZ25_9TELE</name>
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        <submittedName>
            <fullName evidence="7">Cytochrome b-c1 complex subunit 2, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115424526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BZ25"/>
    <dbReference type="FunCoup" id="A0A673BZ25">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049431.1">
        <property type="protein sequence ID" value="ENSSORP00005048241.1"/>
        <property type="gene ID" value="ENSSORG00005020619.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BZ25"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome b-c1 complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquinol-cytochrome c reductase core subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF226">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="5">
        <location>
            <begin position="52"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="6">
        <location>
            <begin position="203"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="478" mass="51025" checksum="A54C9F97B9DCC38D" modified="2020-06-17" version="1">SIIFRIFIKRCYAAARRSEPLTEPLSGRKPSPAAPLPPQNVQVSKLPNGLVIASLESYSPLASVSLFVRAGSRYETVENSGVAHVLRLAANLTTKGASAFKICRGVEALGGSLSVTSSRETMVYSADCLRDNLDSLLEYLVNVTTAQEFRPWEVAELTSRVNIDKTLAQQCPQIGVIERLHEAAYKNALSNSLYCPDYMVGRVSPQQLQSFVDENFTTGRMALVGLGVKHSTLRQVGEGLLSARSGPGAPVAKAIYRGGEARIHSNDNLVHSLIVSEGGVAGSAEANAFSVLQRVLGAGPHVKRGSNITSKLSQGIAKATTQPFDVTAFNASYSDSGLFGVYTISQADAAGEVIKAAISQLRGVAEGNASDADITRAKNQVKAEYLMSIESSEGLIEEIGAQALTTATYQVPETVLQAIDAVTHDDVIKASDCMTCGVFSTSIAIIPNTPIFITLSIICFKHKSVMMHESHLNQQTPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0D5</accession>
    <name>A0A673C0D5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylyl cyclase-activating protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Guanylate cyclase activator 1B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115437066</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030016015.1">
        <property type="nucleotide sequence ID" value="XM_030160155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C0D5"/>
    <dbReference type="Ensembl" id="ENSSORT00005047900.1">
        <property type="protein sequence ID" value="ENSSORP00005046734.1"/>
        <property type="gene ID" value="ENSSORG00005021385.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437066"/>
    <dbReference type="InParanoid" id="A0A673C0D5"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120199">
        <property type="term" value="C:cone photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008048">
        <property type="term" value="F:calcium sensitive guanylate cyclase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000052">
        <property type="entry name" value="Guanylate cyclase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF11">
        <property type="entry name" value="GUANYLYL CYCLASE-ACTIVATING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0844">Vision</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="194" mass="22472" checksum="61F3E0AFEA07E74F" modified="2020-06-17" version="1">MGQSQQTENTEEIDIKALQDMYKKFVMECPSGLLFLHEFKRFFGVDPTGEASEYAENMFRAFDKNGDNTIDFLEFVAALNLVFRGDLEHKLRWSFKVYDKDGNGYVDKDELRSIIDSIYRVKKGPKTDINEMQLTLDEVVDRILEAVDADGDGQITVDEFIKGAQQEPWLLNLLKLDMNPATWVMEQRRKSAHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0F3</accession>
    <name>A0A673C0F3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C0F3"/>
    <dbReference type="Ensembl" id="ENSSORT00005047566.1">
        <property type="protein sequence ID" value="ENSSORP00005046407.1"/>
        <property type="gene ID" value="ENSSORG00005021246.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C0F3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026088">
        <property type="entry name" value="Niban-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14392">
        <property type="entry name" value="NIBAN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14392:SF3">
        <property type="entry name" value="PROTEIN NIBAN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26089">
        <property type="entry name" value="PH_Niban2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="18986" checksum="98585A6A8EBE2F00" modified="2020-06-17" version="1">MGASSSGLLDEAKISHIKGLVDSTLQSFSVFYHQQYSAAYCGHLHQEVEPKKEGRGLLLTQRPQYDPEDVLYKGTVKFSCWDEQGKKWKERYTVLRRDYKLEIHDSIETFNRGAAAKLVLQPAGGVVLTTEEESKTLLEKSCAGILNGILHTNTLLKTQPSKCGSTKIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C112</accession>
    <name>A0A673C112_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">itpr1a</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 19">Endoplasmic reticulum membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="19">The ITPR1 structure has a large solenoid CY assembly built around the central helical bundle made of the C-terminal domains from four ITPR1 subunits. The solenoid scaffold includes domains responsible for binding of ligands and regulatory proteins and is connected via an allosteric nexus at the cytosolic-membrane interface to the transmembrane channel assembly. Six transmembrane helices from each subunit form the central ion-conduction pore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the InsP3 receptor family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005047778.1">
        <property type="protein sequence ID" value="ENSSORP00005046617.1"/>
        <property type="gene ID" value="ENSSORG00005021261.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C112"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23287">
        <property type="entry name" value="beta-trefoil_MIR_ITPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.30:FF:000001">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor, type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.30">
        <property type="entry name" value="IP3 receptor type 1 binding core, RIH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF2">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00779">
        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 19" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2231"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2261"/>
            <end position="2279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2307"/>
            <end position="2325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2345"/>
            <end position="2372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2393"/>
            <end position="2415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="2520"/>
            <end position="2543"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="22">
        <location>
            <begin position="92"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="22">
        <location>
            <begin position="211"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="22">
        <location>
            <begin position="359"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1701"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1824"/>
            <end position="1846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1877"/>
            <end position="1899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="2079"/>
            <end position="2099"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="2630"/>
            <end position="2678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1826"/>
            <end position="1841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="2079"/>
            <end position="2095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2699" mass="308479" checksum="B672B41F8EFFDC5B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1E1</accession>
    <name>A0A673C1E1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phytanoyl-CoA 2-hydroxylase interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115425809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C1E1"/>
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        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048267.1">
        <property type="protein sequence ID" value="ENSSORP00005047094.1"/>
        <property type="gene ID" value="ENSSORG00005021553.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1E1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042868">
        <property type="entry name" value="PHYHIP/PHYHIPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045545">
        <property type="entry name" value="PHYIP/PHIPL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15698:SF9">
        <property type="entry name" value="PHYTANOYL-COA HYDROXYLASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15698">
        <property type="entry name" value="PROTEIN CBG15099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19281">
        <property type="entry name" value="PHYHIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phytanoyl-CoA hydroxylase-interacting protein-like C-terminal" evidence="2">
        <location>
            <begin position="135"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="356" mass="40344" checksum="C3D38938DA0F1C7C" modified="2020-06-17" version="1">MHGQSRGQSLSWERIELCNNGKSMDAALSTPCNIQICEVTCDSFRIMWDMTPEDTARATHFFIDLSRKESRDPNRFKHRDVPTKLVAKAVPLPMAVRGHWFLSPRTEYCVAVQTAVRQPDGDYLVSEWSQVVEFCTGDYAVEHLQQLLDKAKGSTGRLLKFSVFYRNQHPEYFDYVRSQLNMMMFPWFRNGSHGSPINGKLHGVFFSCNTEFDTGLPPKDSPYGPLRFQIPAGHLLNPNISLYFADFYCMYTAYHYVVLVLAPVGSEGDTFCRTRLHMLDLASNPFLTYTAPQLPGEEPQFCHASDVILEVLFTEPVHLDQGSVQQISGHHQLMSLTTANAKKDPSCKVCNISVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C283</accession>
    <name>A0A673C283_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ppp2r3c</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029982504.1">
        <property type="nucleotide sequence ID" value="XM_030126644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C283"/>
    <dbReference type="FunCoup" id="A0A673C283">
        <property type="interactions" value="1257"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047612.1">
        <property type="protein sequence ID" value="ENSSORP00005046453.1"/>
        <property type="gene ID" value="ENSSORG00005021260.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413644"/>
    <dbReference type="CTD" id="55012"/>
    <dbReference type="InParanoid" id="A0A673C283"/>
    <dbReference type="OrthoDB" id="10265007at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001782">
        <property type="term" value="P:B cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030865">
        <property type="term" value="P:cortical cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045579">
        <property type="term" value="P:positive regulation of B cell differentiation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of dephosphorylation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043029">
        <property type="term" value="P:T cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21505">
        <property type="entry name" value="PPP2R3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000091">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039865">
        <property type="entry name" value="PPP2R3C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PP2A regulatory subunit B'' EF-hand" evidence="9">
        <location>
            <begin position="184"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="457" mass="53787" checksum="F7079B9E7A6C5AB4" modified="2020-06-17" version="1">MEKDEQLHWSDILKRRLENSRKDERSEEEKKAEETELFTKYYTEWKGGDDKDKSYKKIPRFYYRLPAEDEVLLQKLREESRAVFLQRKSRELLDNEELQNLWFLLDKHQVPPVTGEEAMISYEAYLQVGEKAGPKGRKFFTARVYAKLLHNDPYGRISIMQFFNYVMRKVWLHQTRIGLSLYDVAGQGYLRESDLENYILELIPTLPQLDGLEKSFYSFYVCTAVRKFFFFLDPLRTGKIKIQDILACSFLDDLLELRDEDLSKESQESNWFSAPSALRVYGQYLNLDKDHNGMLSKEELSRYGTATLTSVFLDRVFQECLTYDGEMDYKTYLDFVLALENRKEPAALQYIFKLLDMENQGYLNVFSLNYFFRAIQEQMKLHGQEPVSFQDVKDEIFDMVKPKDPYKITLQDLVNSCQGDTVTSILIDLNGFWTYENREVLVANDSTSSNTGDLDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2F7</accession>
    <name>A0A673C2F7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polyadenylate-binding protein</fullName>
            <shortName evidence="9">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pabpc4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Binds and protects the poly(A) tail of mRNA with or without an AU-rich element (ARE) and prevents mRNA deadenylation. Stimulates the translation of mRNAs to which it is bound during early development.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with dazl in an RNA-independent manner. The C-terminus can self-associate and also interact with the C-terminus of pabpc1, independently of RNA. RRM 1 and RRM 2 interact with both eif4g1 and paip1, and the C-terminus also interacts with paip1. Prior to oocyte maturation, found in a complex with dazl and pum2 proteins and spdy1 mRNA; pum2 dissociates from the complex during maturation. Interacts with the translation termination factor sup35/erf3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C2F7"/>
    <dbReference type="FunCoup" id="A0A673C2F7">
        <property type="interactions" value="1704"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048305.1">
        <property type="protein sequence ID" value="ENSSORP00005047132.1"/>
        <property type="gene ID" value="ENSSORG00005021474.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C2F7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000001">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000021">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000042">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000049">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="99"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="191"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="294"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="12">
        <location>
            <begin position="530"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="433"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="477"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="625" mass="69666" checksum="24168C551F5E517A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3G8</accession>
    <name>A0A673C3G8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dipeptidyl-peptidase IV</fullName>
            <ecNumber evidence="3">3.4.14.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115436959</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.</text>
            <dbReference type="EC" id="3.4.14.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.5" evidence="3"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051094.1">
        <property type="protein sequence ID" value="ENSSORP00005049891.1"/>
        <property type="gene ID" value="ENSSORG00005021254.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C3G8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.140.10.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 8-like isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000016">
        <property type="entry name" value="Dipeptidyl peptidase 8-like isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045785">
        <property type="entry name" value="Dpp_8/9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF193">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF19520">
        <property type="entry name" value="Dpp_8_9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Dipeptidyl peptidase 8 /9 ,N-terminal" evidence="10">
        <location>
            <begin position="33"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="9">
        <location>
            <begin position="172"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="694"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="894" mass="102174" checksum="DABA2A7859F8B204" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4K8</accession>
    <name>A0A673C4K8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Galactose-specific lectin nattectin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115434948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030012950.1">
        <property type="nucleotide sequence ID" value="XM_030157090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C4K8"/>
    <dbReference type="FunCoup" id="A0A673C4K8">
        <property type="interactions" value="841"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049378.1">
        <property type="protein sequence ID" value="ENSSORP00005048189.1"/>
        <property type="gene ID" value="ENSSORG00005021998.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434948"/>
    <dbReference type="InParanoid" id="A0A673C4K8"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01504">
        <property type="entry name" value="PNCREATITSAP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025690237" evidence="1">
        <location>
            <begin position="21"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="38"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="159" mass="18006" checksum="1BB85820F7F26B0B" modified="2020-06-17" version="1">MASTLFVIAFLCGLWIEANAQTDPGDFCQECPPGWTTFDKRCYLFHKQEKDWADAERTCTSLGANLASIRNAGVYKFLRQMILRVTGSNKKTWAGGYDAPKEGVWLWSDGFHFDFKAWPKGEPNNLGGEHCMEFNLRGKDYVNDAKCTTRNSFICAKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6N7</accession>
    <name>A0A673C6N7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ankyrin repeat and SOCS box containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">asb3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C6N7"/>
    <dbReference type="Ensembl" id="ENSSORT00005052370.1">
        <property type="protein sequence ID" value="ENSSORP00005051146.1"/>
        <property type="gene ID" value="ENSSORG00005023118.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C6N7"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051637">
        <property type="entry name" value="Ank_repeat_dom-contain_49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 2C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24180:SF45">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE TANKYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="215"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="283"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="455"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="507" mass="55514" checksum="03CD6E0206479448" modified="2020-06-17" version="1">MNFTEVYGDTVSSVALAARSGCRKQVRRLIQRGGSVDCRDNRGWNSLHEAAAAGNEGCVREILKAAARSSRSFRAFVNSLTHEGESSCYLAAQRGHMDVVRLLLKNHAVVNQQTNDLSCPLYAAVDAGHVEVVRLLVREGAEVNGTHTASCWTCLHQAVYKGHSDIVRILVSVSDLEAVDDHNITPLFVAAQYGRHDDLQTLAFADANVDAQAADGATPLMIASQEGHESCVDFLLGCGANPNTPCSSEWPQLPIHAAAQFGRISVLRRLIAATDRGCDRGRGQVSPLYLAIHNNQRTSVELLLKEGFSPDAQDCWELLGLRSPLLVSAGASLIQDHWDQVLAADRTDLLRLILDLRRIPHLDLAPCHRPEAWGPAVLTLPELKHLLSAAVTQVDAAARWLPLLLNAGLDPHLLLLDGLDGAVLNYLLQFVNWSTLSLDLKLVLDRRQAEKTWSPLPQFDSLPSLFHLCRMRIRTLLGPDLLMRTAVVQQLPLPSPLHAVLLTAHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C785</accession>
    <name>A0A673C785_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Claudin 15-like b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C785"/>
    <dbReference type="Ensembl" id="ENSSORT00005049291.1">
        <property type="protein sequence ID" value="ENSSORP00005048103.1"/>
        <property type="gene ID" value="ENSSORG00005021960.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C785"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="121" mass="12849" checksum="634C3BAFE32F0EEF" modified="2020-06-17" version="1">MSCVATTTGSVQCNRFRTVLGLPAHLQACRALMIMSLLIGLAAIIVSVLGLKCTKIGRTSDQAKAQMVLTGGIMVILSGLLTLTAVSLYAAQVIHDFYDPLYGGVRSVLLQTVDTTEYSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7N7</accession>
    <name>A0A673C7N7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pde4c</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A673C7N7"/>
    <dbReference type="Ensembl" id="ENSSORT00005052793.1">
        <property type="protein sequence ID" value="ENSSORP00005051561.1"/>
        <property type="gene ID" value="ENSSORG00005023287.1"/>
    </dbReference>
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        <property type="reaction ID" value="UER00747"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDIESTERASE1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0114">cAMP</keyword>
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            <begin position="276"/>
            <end position="605"/>
        </location>
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            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="600"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="617"/>
            <end position="641"/>
        </location>
    </feature>
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        <location>
            <position position="352"/>
        </location>
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        <location>
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            <end position="356"/>
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        <ligand>
            <name>AMP</name>
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        </ligand>
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            <position position="356"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <position position="392"/>
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        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
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            <position position="393"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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            <label>2</label>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
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        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>AMP</name>
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        <ligand>
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            <label>1</label>
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            <position position="561"/>
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        <ligand>
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        </ligand>
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            <dbReference type="ARBA" id="ARBA00004703"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        </source>
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        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="696" mass="78370" checksum="DFBCB359B7EE3AB9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C991</accession>
    <name>A0A673C991_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Alpha-1A adrenergic receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alpha-1A adrenoreceptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
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        </lineage>
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    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADR_fam"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF16">
        <property type="entry name" value="ALPHA-1A ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADRENERGICR"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="85"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="105"/>
            <end position="125"/>
        </location>
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            <begin position="146"/>
            <end position="167"/>
        </location>
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            <end position="212"/>
        </location>
    </feature>
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            <end position="303"/>
        </location>
    </feature>
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        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="46"/>
            <end position="331"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014216"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="409" mass="45159" checksum="C08EFB8A19526B26" modified="2020-06-17" version="1">MCRIKIMISGSFSMTFYSSTYPQVDVGKAVVLGLVMVVFLVFGVLGNILVILSVLFHHHWRSVTHYFIVNLAAADLLLSCAVMPFSAASEALGRWEFGRSFCSVWAALDVLCCTASILSLCVISIDRYLAVSYPLRYPAIATGRRGLAAVAALWGVSAIISVGPLFGWKEPDPEDETVCQITEEPGYALFSALGSFYIPLAIILAMYCRVYTVATRETKSLRKSSKGEGVETEGVMLRMHRGNAPQMDNKNTNVKHKRSSFTLQKLLKFSREEKAAKTLGIVVGCFVLCWLPFFLVLPIGSFFPSCKPSETVFKITFWLGYLNSCINPIIYPCFSQEFKKAFLNVLRVRCLRTASQTSIQGHVIAYSSGSGHASSTSVLSTQNHDTVSSCGCCGADFFNKLPRHNFFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCC7</accession>
    <name>A0A673CCC7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">G2/M-phase specific E3 ubiquitin protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">g2e3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCC7"/>
    <dbReference type="FunCoup" id="A0A673CCC7">
        <property type="interactions" value="1201"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052165.1">
        <property type="protein sequence ID" value="ENSSORP00005050949.1"/>
        <property type="gene ID" value="ENSSORG00005023053.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCC7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15669">
        <property type="entry name" value="ePHD_PHF7_G2E3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051188">
        <property type="entry name" value="PHD-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059102">
        <property type="entry name" value="PHD_PHF7/G2E3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042013">
        <property type="entry name" value="PHF7/G2E3_ePHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420:SF42">
        <property type="entry name" value="G2_M PHASE-SPECIFIC E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26054">
        <property type="entry name" value="PHD_G2E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="11"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="701" mass="78004" checksum="833C27DDF088AAF9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCD4</accession>
    <name>A0A673CCD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proprotein convertase subtilisin/kexin type 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="33">Proprotein convertase 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Subtilisin/kexin-like protease PC9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="40" type="primary">pcsk9</name>
    </gene>
    <organism evidence="40 41">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="40" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="40" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="40" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer. Can self-associate to form dimers and higher multimers which may have increased LDLR degrading activity. The precursor protein but not the mature protein may form multimers. Interacts with APOB, VLDLR, LRP8/APOER2 and BACE1. The full-length immature form (pro-PCSK9) interacts with SCNN1A, SCNN1B and SCNN1G. The pro-PCSK9 form (via C-terminal domain) interacts with LDLR. Interacts (via the C-terminal domain) with ANXA2 (via repeat Annexin 1); the interaction inhibits the degradation of LDLR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 34">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCD4"/>
    <dbReference type="FunCoup" id="A0A673CCD4">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050986.1">
        <property type="protein sequence ID" value="ENSSORP00005049783.1"/>
        <property type="gene ID" value="ENSSORG00005022584.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCD4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000004">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000016">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.690">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041254">
        <property type="entry name" value="PCSK9_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041051">
        <property type="entry name" value="PCSK9_C3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF60">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18459">
        <property type="entry name" value="PCSK9_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18463">
        <property type="entry name" value="PCSK9_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="21" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="24" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="27" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="19" id="KW-0967">Endosome</keyword>
    <keyword evidence="29" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="25" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20 34" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="26" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="31" id="KW-0458">Lysosome</keyword>
    <keyword evidence="15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17 34" id="KW-0645">Protease</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0964">Secreted</keyword>
    <keyword evidence="23 34" id="KW-0720">Serine protease</keyword>
    <keyword evidence="35" id="KW-0732">Signal</keyword>
    <keyword evidence="30" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="28" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="16" id="KW-0765">Sulfation</keyword>
    <feature type="signal peptide" evidence="35">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025679285" description="Proprotein convertase subtilisin/kexin type 9" evidence="35">
        <location>
            <begin position="31"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="37">
        <location>
            <begin position="83"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="36">
        <location>
            <begin position="185"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Proprotein convertase subtilisin/kexin type 9 C-terminal" evidence="38">
        <location>
            <begin position="449"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Proprotein convertase subtilisin/kexin type 9 C-terminal" evidence="39">
        <location>
            <begin position="610"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="34">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="34">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="34">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00011841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00019781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00032525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00032870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="Pfam" id="PF18459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="Pfam" id="PF18463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="692" mass="73241" checksum="890497911A2CDED2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CD66</accession>
    <name>A0A673CD66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytoplasmic phosphatidylinositol transfer protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Retinal degeneration B homolog beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pitpnc1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidic acid (PA) between membranes. Binds PA derived from the phospholipase D signaling pathway and among the cellular PA species, preferably binds to the C16:0/16:1 and C16:1/18:1 PA species.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate(in) = a 1,2-diacyl-sn-glycero-3-phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:36435"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:36436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PtdIns transfer protein family. PI transfer class IIB subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CD66"/>
    <dbReference type="Ensembl" id="ENSSORT00005052479.1">
        <property type="protein sequence ID" value="ENSSORP00005051254.1"/>
        <property type="gene ID" value="ENSSORG00005023173.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CD66"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08890">
        <property type="entry name" value="SRPBCC_PITPNC1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000011">
        <property type="entry name" value="cytoplasmic phosphatidylinositol transfer protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF55">
        <property type="entry name" value="CYTOPLASMIC PHOSPHATIDYLINOSITOL TRANSFER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="341" mass="39351" checksum="F3CAD02061AC09A0" modified="2020-06-17" version="1">MMCEVYVSTCVLFILQYRIGQLYMISKHSHEQSDRGEGVEVVQNEPYEDPTHGQGQFTEKRVYLNSKLPSWARAVVPKIFYVTEKAWNYYPYTITGEYYHINCSFLPKFSIHIETKYEDNKGCNDNIFDTELKEQEREVCFVDIAYDEIPERYYKESEDLRYFKSEKTSRGILQEGWRDTQDPIMCSYKLVTVKFEVWGLQTRVEQFVHKVIRDVLLLGHRQAFAWVDEWIDMTMDEVREFERTIQEATNQKIGMFPPSISVNATPLSSFALTGPASAPSTPMCTDAPESLSVPKDRPRKKSAPETLTLPEPVPESPVSVSNHLQSDASPSSTLDIEEMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGB5</accession>
    <name>A0A673CGB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serum amyloid A like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">saal1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAAL1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029992756.1">
        <property type="nucleotide sequence ID" value="XM_030136896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CGB5"/>
    <dbReference type="FunCoup" id="A0A673CGB5">
        <property type="interactions" value="1239"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053595.1">
        <property type="protein sequence ID" value="ENSSORP00005052349.1"/>
        <property type="gene ID" value="ENSSORG00005023596.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421161"/>
    <dbReference type="CTD" id="113174"/>
    <dbReference type="InParanoid" id="A0A673CGB5"/>
    <dbReference type="OrthoDB" id="2156856at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901647">
        <property type="term" value="P:positive regulation of synoviocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001132">
        <property type="entry name" value="Serum amyloid A-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052464">
        <property type="entry name" value="Synovial_Prolif_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424:SF23">
        <property type="entry name" value="PROTEIN SAAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424">
        <property type="entry name" value="SERUM AMYLOID A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="466" mass="51802" checksum="2B4C4D1869E76888" modified="2020-06-17" version="1">MDNNSDRVDNVDNEKSSSSKGTQSPVMDRNPSPPPDSDGEEAEEALDAIGDTVYSKHWLFSTLTHLINMVAEHSEEDSDGQVQLSDDDEENLCRVWDMAMDKDVAAFLQEFKATDILLGVIAKSRCPRLTEICVGILGNIACFSDTCLTLSQNEDLGAVLLLLLGDADPPTLLETSRLLLTCVSQKDACPIWLQRIRQRTVVRSNLCFIMCSSTNCDLLEKVGELVEKLFDLDEELMKSWITAQPSEEDDEGETHLDMASCLLEAAKQLRSENPNGLEVYLHALQLFTTVDEGIEIFAAADGPGKAVWDFVSEVVCEDLCQSNDLPVVLLEQKGVLVQTFAVLQALYKCQDQWSSRSDTSLPLIATLLRVLQYHTECKRYCEADAKDEQLQTLAEITAEFLAEICIQIPKDTVTDLIKTGHLTEKTCVTAAGWLHTQFKTSFQHLQSVLSEADPQMADVVRKQFPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CHG9</accession>
    <name>A0A673CHG9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Dynein axonemal heavy chain 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">dnah10</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A673CHG9">
        <property type="interactions" value="558"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005051503.1"/>
        <property type="gene ID" value="ENSSORG00005023266.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CHG9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_11_sf"/>
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        <property type="entry name" value="DHC"/>
    </dbReference>
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        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
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        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
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        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
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        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
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        <property type="entry name" value="Dynein_C"/>
    </dbReference>
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        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
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        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
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        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
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        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 10"/>
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    </dbReference>
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        <property type="entry name" value="AAA_7"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AAA_9"/>
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    </dbReference>
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        <property type="entry name" value="AAA_lid_11"/>
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    </dbReference>
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        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
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    </dbReference>
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        <property type="entry name" value="Dynein_C"/>
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    </dbReference>
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        <property type="entry name" value="Dynein_heavy"/>
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    </dbReference>
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        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="1845"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2124"/>
            <end position="2272"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2465"/>
            <end position="2618"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2813"/>
            <end position="2970"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3062"/>
            <end position="3092"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3279"/>
            <end position="3369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="4485" mass="514443" checksum="59E4DE32F95760C4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJA5</accession>
    <name>A0A673CJA5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Erythrocyte band 7 integral membrane protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">stom</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJA5"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054619.1">
        <property type="protein sequence ID" value="ENSSORP00005053364.1"/>
        <property type="gene ID" value="ENSSORG00005023970.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJA5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03403">
        <property type="entry name" value="SPFH_stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000002">
        <property type="entry name" value="band 7 protein AGAP004871"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF115">
        <property type="entry name" value="STOMATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="5">
        <location>
            <begin position="66"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="32889" checksum="8A25AFDA4FAD09B4" modified="2020-06-17" version="1">MVPCDFLMDLHCIIGTFPSVKRTQIVQRCKMYSLENTDNELGLCGWLLVLFSLLLVLVTLPLSIWMCIKIVKEYERAIIFRLGRILRGGAKGPGLFFIVPCTDSFINVDMRTITFDIPPQEVLTKDSVTVSVDGVVYYRVQNATLAVANITNADAATRLLAQTTLRNVLGTKNLAEILSDREEIAHSMQSTLDDATDDWGIKVERVEIKDVKLPLQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASLVIAESPSALQLRYLQTLNTIAAEKNSTIIFPLPLDMMQAFIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJE1</accession>
    <name>A0A673CJE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UPF3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">upf3b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RENT3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002067.1">
        <property type="nucleotide sequence ID" value="XM_030146207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CJE1"/>
    <dbReference type="FunCoup" id="A0A673CJE1">
        <property type="interactions" value="891"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056997.1">
        <property type="protein sequence ID" value="ENSSORP00005055711.1"/>
        <property type="gene ID" value="ENSSORG00005024842.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427605"/>
    <dbReference type="CTD" id="65109"/>
    <dbReference type="InParanoid" id="A0A673CJE1"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12728">
        <property type="entry name" value="RRM_like_Smg4_UPF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000067">
        <property type="entry name" value="regulator of nonsense transcripts 3A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034979">
        <property type="entry name" value="UPF3B_RRM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF1">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="UPF3" evidence="9">
        <location>
            <begin position="34"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="196"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="299"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="461" mass="55812" checksum="640B1584BA9F246B" modified="2020-06-17" version="1">MKEDKENTRPKDKKVDIKCEDGEKMEKSKEKKEAMTKIVIRRLPPSLTKEELEEQLQPLPEVDYLEFFSNDTSLYPHLFARAYINFKNQEDIVLFRDRFDGYVFIDNRGQEYPAIVEFAPFQKTAKKRVKKKDAKCGTITEDPDYKKFLEYYNGDEEKFTSTPETLLEEIEAKSKELGGKKTTPLLDFLKNKQRLREEKKEERRRRELERKRLRDEERRKWREEERKKRKEAEKMKRLEKPLEKDKDHIKEEPKIKLLKKPERSDDGDSEKPKEKNKKPEKPNKEDRSMGSADQKRRHNIDNKEDRGRKADEDGRKEFRERDTDRDRERDREKERRQREKERIRRQDEDRRRRRERQDGENTYRKREEEFKKEKDRVSEKKKGEHMADSTHSERPEKHAKDNKKEESVKRERLRNKDRPAIQLYQPGARSRNRVTGGGDELKTADKKPDTETKKVSDKGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLF4</accession>
    <name>A0A673CLF4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC115434592</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">si:ch211-248a14.8</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CLF4"/>
    <dbReference type="Ensembl" id="ENSSORT00005055406.1">
        <property type="protein sequence ID" value="ENSSORP00005054134.1"/>
        <property type="gene ID" value="ENSSORG00005024269.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLF4"/>
    <dbReference type="OrthoDB" id="9943635at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="329" mass="35879" checksum="AC826E9EF2F83876" modified="2020-06-17" version="1">MKCDVMLRIDPWEKGPALAQNERLIGKSAFSPTGCCASAVKGLSSNHALKPLVPTLCLVVVVIYGLGDKLRNFVAGIFIPQYHYPYAVALCFAQVVISLLVLNLLHVLDLVPLKPYSRPLGEKVLVPSISCSIQSVLTMWAKDSSSCTGLFPLLMPLLPLLTAALSYSLKLAPPPSIHISVLVSFLSGICVVITASRGLSDMEPLEYIYAPAALILHSLSLTWLAKVSEAERHHSPDSKASIFDIYYTGLVNQSWVLGLLWLLHPHSPWQVLSRGSWHSLLFHGYLLAILLLGMVLDFLVGIVALSFSPVAAATLHSARELVQPFFHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLN5</accession>
    <name>A0A673CLN5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CLN5"/>
    <dbReference type="Ensembl" id="ENSSORT00005054192.1">
        <property type="protein sequence ID" value="ENSSORP00005052938.1"/>
        <property type="gene ID" value="ENSSORG00005023851.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLN5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="104" mass="11811" checksum="1CD0F63AA7A766DA" modified="2020-06-17" version="1">MNSSPWWIVCALHVSNCIHQVFQEKKNHTDHIEDVTTLCPTVFPMSSAAGTVSVPWALVIFLRLSTINCRNKICIFKTGHKTLLQESQCELVQGSRTVEAQHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLQ0</accession>
    <name>A0A673CLQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CLQ0"/>
    <dbReference type="FunCoup" id="A0A673CLQ0">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054207.1">
        <property type="protein sequence ID" value="ENSSORP00005052953.1"/>
        <property type="gene ID" value="ENSSORG00005023855.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLQ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF188">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="9"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="204"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="258" mass="29121" checksum="24BE21624AE9322D" modified="2020-06-17" version="1">CGGLCKKDPDCLRIVLIGKTGSGKSSSGNTILGREEFKADFSQTSVTKVCRKVPDQVDGRPVAVVDTPGLFDPNLTNKQVHEEVVKCISLLAPGPHVFLLVLEIGRFTPEEKETLKLIKEGFGKNAEKFIIILLTHGDKLETRGISIDDYIRDKCDDSFKKLISDCGGRYHVFNNQDKNPTQVNELMKKIDSMVKENRGSCFTNKMLQEAEAAIKKEMERLLKEKEEEMRKQKEDFVAEHQNFPHRHGHTFQGNVTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CNL7</accession>
    <name>A0A673CNL7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Occludin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">oclna</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CNL7"/>
    <dbReference type="FunCoup" id="A0A673CNL7">
        <property type="interactions" value="1027"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057143.1">
        <property type="protein sequence ID" value="ENSSORP00005055855.1"/>
        <property type="gene ID" value="ENSSORG00005024900.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CNL7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002958">
        <property type="entry name" value="Occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF4">
        <property type="entry name" value="OCCLUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01258">
        <property type="entry name" value="OCCLUDIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12 14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <keyword evidence="8 14 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="18">
        <location>
            <begin position="42"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="OCEL" evidence="19">
        <location>
            <begin position="379"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="288"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="293"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="486" mass="54600" checksum="46AD35EBE27CAFD1" modified="2020-06-17" version="1">MSSRPNGSPPPYESENGYNVPPQPAYSYYPDDEFQHFYRWTSPPGILKIMGIIVIVLCVAIFACVASTLAWDTQGVGLGGSYGNNYGSFGGGGYGPGNNYGYGGIGGNYNDPRKGKGFMIAMAAIVFIFALVVFIIIVSHQSLSQSRRFYLALVIICAILALLMLIATIVYLVAVNPMSQSYGSAYGNQIAGLCAQYQQPQPSGAFINQYLYHYCVVEPQEAIAIVFGFLVTGALIVMLVFALMTRKRIQEYGKSNILWRRVKVIDDNAPPQDVEAWVNNVSVPPQEIPISDYPEKLRSSRSHLDDESSNYDKPPYSYTPQPAMEEERPLHNGAPYSSTSEIASSAGRPKKRRAGRPRRTDGQDFDTDYASSGDELDDNDFDSEFPPIANDQDRNNYKREFDRDHQEYKDLQAELDAINKNLSEVDRELDELEEGSPQYLDALDEYNRIKKVKKSADYQMKKRRCKYLKGKLNYIKKLVSDYDRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQ55</accession>
    <name>A0A673CQ55_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Beta-galactosidase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">glb1l</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CQ55"/>
    <dbReference type="Ensembl" id="ENSSORT00005055988.1">
        <property type="protein sequence ID" value="ENSSORP00005054712.1"/>
        <property type="gene ID" value="ENSSORG00005024130.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CQ55"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000021">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000017">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026283">
        <property type="entry name" value="B-gal_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048912">
        <property type="entry name" value="BetaGal1-like_ABD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21317">
        <property type="entry name" value="BetaGal_ABD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006336">
        <property type="entry name" value="B-gal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025473583" evidence="8">
        <location>
            <begin position="26"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="9">
        <location>
            <begin position="38"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase 1-like first all-beta" evidence="10">
        <location>
            <begin position="402"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase galactose-binding" evidence="11">
        <location>
            <begin position="545"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="649" mass="73324" checksum="4D207856583764F8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRU1</accession>
    <name>A0A673CRU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">A-type potassium channel modulatory protein DPP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Dipeptidyl aminopeptidase-like protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Dipeptidyl peptidase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Dipeptidyl peptidase VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115411374</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer (in vitro). Interacts with KCND2. Identified in a complex with KCND2 and KCNIP2. Forms an octameric complex composed of four DPP6 subunits bound to the KCND2 tetramer. Interacts with KCND3; this interaction modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CRU1"/>
    <dbReference type="Ensembl" id="ENSSORT00005059679.1">
        <property type="protein sequence ID" value="ENSSORP00005058356.1"/>
        <property type="gene ID" value="ENSSORG00005025761.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CRU1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF20">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="17">
        <location>
            <begin position="128"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="16">
        <location>
            <begin position="554"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="774" mass="88281" checksum="74AD382E052CFEF3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSN3</accession>
    <name>A0A673CSN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">NAD(P)H oxidoreductase RTN4IP1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115415032</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-demethylubiquinone-10 + NADH + 2 H(+) = 3-demethylubiquinol-10 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:83243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64182"/>
            <dbReference type="ChEBI" id="CHEBI:231824"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-demethylubiquinone-10 + NADPH + 2 H(+) = 3-demethylubiquinol-10 + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:83247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64182"/>
            <dbReference type="ChEBI" id="CHEBI:231824"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-demethylubiquinone + NADH + 2 H(+) = a 3-demethylubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:83235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 3-demethylubiquinone + NADPH + 2 H(+) = a 3-demethylubiquinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:83239"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984300.1">
        <property type="nucleotide sequence ID" value="XM_030128440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CSN3"/>
    <dbReference type="Ensembl" id="ENSSORT00005056835.1">
        <property type="protein sequence ID" value="ENSSORP00005055552.1"/>
        <property type="gene ID" value="ENSSORG00005024794.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415032"/>
    <dbReference type="InParanoid" id="A0A673CSN3"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08248">
        <property type="entry name" value="RTN4I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000147">
        <property type="entry name" value="Reticulon-4-interacting protein 1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000009">
        <property type="entry name" value="Reticulon-4-interacting protein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037397">
        <property type="entry name" value="RTN4IP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025602460" description="NAD(P)H oxidoreductase RTN4IP1, mitochondrial" evidence="20">
        <location>
            <begin position="22"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="21">
        <location>
            <begin position="58"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="404"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00071154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="435" mass="46882" checksum="72EABF85D8581C3F" modified="2020-06-17" version="1">MGTVRAMASARFMCFLNAASSTKTLARTGCSVCFRRYICSSPPRSQSCMSAWVIDQYGTNGVLRYTEEITAPTIKSATDVMIKVCAASLNPLDMSMRGGYGSKLLKLRRDPLSMMNNESEFPLILGRDVSGVVVDCGSEVTYVAPGDEVWASVPPWKQGSLAEFVTLSEYEVSHKPKLLSHIEAASIPYVANTALSALVNTGGLCRDSASNKRVLITGGSGGVGTFSIQLLKAWGAHVTVTCSQNAEGLVRGLGADEVVDYTAGDVAEQLEKMERFDVILDGVGGDTEQWALGLLKPWSGAKYVTLVTPLLFSMDSMGLFGGAINAGVTLHNKAIQGVLSNGVFYRWGFYAPDGPALDEVSRLVDSGKILPVVEAHFPFTQVPQAFLKLEQGHARGKTVVTVAEEDSRQADEMAQRTYTDTAETEQEMQETLKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CW28</accession>
    <name>A0A673CW28_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">C-C chemokine receptor type 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ccr6b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CW28"/>
    <dbReference type="Ensembl" id="ENSSORT00005057446.1">
        <property type="protein sequence ID" value="ENSSORP00005056153.1"/>
        <property type="gene ID" value="ENSSORG00005025000.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CW28"/>
    <dbReference type="OrthoDB" id="9828427at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000035">
        <property type="entry name" value="C-C chemokine receptor type 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF943">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="49"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="362" mass="40797" checksum="BC3A1142CB8E985B" modified="2020-06-17" version="1">MTTSTPDYDYNFTDPTDSPDEPCSFQAEPVEVIIQTYIHSFICAFGLIGNVLVIVTYLFYKRTKTMTDVYLFNVAVADLIFVVALPFIIYNEQNSWLMGPVSCKLLMSAYSINLYSGMLLLACISSDRYVAIVQARRTFGTRSRNLFYSRLICSAVWVFAVALTLPTFIYTERFEVWNLGGDSVTIMCQLSFNKSETAKLMKILVPSLQMAIGFLLPLLVMIICYSCILCTLLRAQSSQKHKAVRVVFAVVLVFIICHLPYNVALLSHTVALFTVRSCDMEKLKLHVLTISRSVAYLHCCLNPILYAFVGVKFRSHFRQIMLDLWCVSKKYISSARSSRATSEIFVSGIKTSDASNMSSFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673D036</accession>
    <name>A0A673D036_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Tensin 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">tns3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673D036"/>
    <dbReference type="Ensembl" id="ENSSORT00005059284.1">
        <property type="protein sequence ID" value="ENSSORP00005057963.1"/>
        <property type="gene ID" value="ENSSORG00005025627.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673D036"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01213">
        <property type="entry name" value="PTB_tensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09927">
        <property type="entry name" value="SH2_Tensin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000039">
        <property type="entry name" value="Tensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000002">
        <property type="entry name" value="Tensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000010">
        <property type="entry name" value="tensin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035012">
        <property type="entry name" value="Tensin-like_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033929">
        <property type="entry name" value="Tensin_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF5">
        <property type="entry name" value="TENSIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="15">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="103"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="16">
        <location>
            <begin position="175"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="13">
        <location>
            <begin position="955"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="12">
        <location>
            <begin position="1096"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="339"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="489"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="542"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="596"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="777"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="852"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="380"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="547"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="793"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="806"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="867"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1229" mass="135009" checksum="145CCB5A6F68DB37" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y259</accession>
    <name>A0A672Y259_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115431459</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030007697.1">
        <property type="nucleotide sequence ID" value="XM_030151837.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030007706.1">
        <property type="nucleotide sequence ID" value="XM_030151846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y259"/>
    <dbReference type="SMR" id="A0A672Y259"/>
    <dbReference type="Ensembl" id="ENSSORT00005000094.1">
        <property type="protein sequence ID" value="ENSSORP00005000082.1"/>
        <property type="gene ID" value="ENSSORG00005000069.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431459"/>
    <dbReference type="InParanoid" id="A0A672Y259"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032486">
        <property type="term" value="P:Rap protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04176">
        <property type="entry name" value="Rap2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000189">
        <property type="entry name" value="Member of ras oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041840">
        <property type="entry name" value="Rap2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="183" mass="20693" checksum="B36E829713822E74" modified="2020-06-17" version="1">MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIRNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYQQVPVVLVGNKVDLEDEREVSPSEGQALAEDWGCPFMETSAKSKTMVDELFAEIVRQMDFCPLPDRREACCPACSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3F1</accession>
    <name>A0A672Y3F1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein spinster homolog 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Spns1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">spns1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(out) + H(+)(out) = a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74447"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(out) + H(+)(out) = a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74455"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine(out) + H(+)(out) = a 1-acyl-sn-glycero-3-phosphocholine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74435"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphoethanolamine(out) + H(+)(out) = a 1-acyl-sn-glycero-3-phosphoethanolamine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64381"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y3F1"/>
    <dbReference type="FunCoup" id="A0A672Y3F1">
        <property type="interactions" value="1030"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000738.1">
        <property type="protein sequence ID" value="ENSSORP00005000712.1"/>
        <property type="gene ID" value="ENSSORG00005000429.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y3F1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
        <property type="entry name" value="MFS_spinster_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF13">
        <property type="entry name" value="PROTEIN SPINSTER HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025609980" description="Protein spinster homolog 1" evidence="17">
        <location>
            <begin position="26"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="18">
        <location>
            <begin position="66"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="512" mass="56161" checksum="6E65F10A8727698F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9U0</accession>
    <name>A0A672Y9U0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 13">5-hydroxyisourate hydrolase</fullName>
            <shortName evidence="13">HIU hydrolase</shortName>
            <shortName evidence="13">HIUHase</shortName>
            <ecNumber evidence="6 13">3.5.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">urahb</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Purine metabolism; urate degradation; (S)-allantoin from urate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.17" evidence="6 13"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y9U0"/>
    <dbReference type="FunCoup" id="A0A672Y9U0">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000745.1">
        <property type="protein sequence ID" value="ENSSORP00005000719.1"/>
        <property type="gene ID" value="ENSSORG00005000459.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y9U0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.180:FF:000004">
        <property type="entry name" value="5-hydroxyisourate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000895">
        <property type="entry name" value="Transthyretin/HIU_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395:SF13">
        <property type="entry name" value="5-HYDROXYISOURATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="URICASE AND TRANSTHYRETIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00189">
        <property type="entry name" value="TRNSTHYRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00095">
        <property type="entry name" value="TR_THY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="8 13" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="14">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600895-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="119" mass="13321" checksum="564596C7636E1E65" modified="2020-06-17" version="1">MAAAESSPLTTHVLNTGDGVPAARMALSLHRLDTNTMIWNMLNVGTTDQDGRCPGLISRDAFTSGTYKLRFETGQYWKSLGQTSFYPYAEVVFTISDPQQRFHIPLIMSRFSYSTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YG03</accession>
    <name>A0A672YG03_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Solute carrier family 8 member 4b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">slc8a4b</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YG03"/>
    <dbReference type="Ensembl" id="ENSSORT00005003593.1">
        <property type="protein sequence ID" value="ENSSORP00005003492.1"/>
        <property type="gene ID" value="ENSSORG00005002066.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YG03"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000001">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000003">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000024">
        <property type="entry name" value="sodium/calcium exchanger 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032452">
        <property type="entry name" value="Na_Ca_Ex_C-exten"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00845">
        <property type="entry name" value="caca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF26">
        <property type="entry name" value="SODIUM_CALCIUM EXCHANGER 4 ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16494">
        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="710"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="787"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="819"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="859"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="21">
        <location>
            <begin position="353"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="21">
        <location>
            <begin position="484"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="886" mass="97882" checksum="00E65B010306A229" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHM4</accession>
    <name>A0A672YHM4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Monocarboxylate transporter 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">slc16a7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>3-methyl-2-oxobutanoate(out) + H(+)(out) = 3-methyl-2-oxobutanoate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71783"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-methyl-2-oxopentanoate(out) + H(+)(out) = 4-methyl-2-oxopentanoate(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71779"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YHM4"/>
    <dbReference type="Ensembl" id="ENSSORT00005004168.1">
        <property type="protein sequence ID" value="ENSSORP00005004048.1"/>
        <property type="gene ID" value="ENSSORG00005001365.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YHM4"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000030">
        <property type="entry name" value="monocarboxylate transporter 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004743">
        <property type="entry name" value="MCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00892">
        <property type="entry name" value="2A0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF92">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="19"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="478" mass="51709" checksum="B3560098D35C5B11" modified="2020-06-17" version="1">MPPAATTSLGYSPPDGGWGWAVVFGSFISIGFSYAFPKSLTIYFKEIQEYFSVSYSEIAWVSSVMLASMYAGGPVSSVLVNRYGSRPVVMVGGVMVGTAMLLASFGTSIMHLYLCVGLIGGFGLAFNLQPALTIIGTYFQVKRPLANGLAMTGSPVILSTLAPLNQFLFDSFGWRGSFLILGSICLNCCVAGSLMRPVTKVVPPKPGPGPPGCNGAAAEEDTRVQSENLQTEEAKSESKSQSCAERFIDFSLFKHRGFLIYLVGNVVLFFGFFAPVVFLAPYAKHQGIDEYSAALLLTIFALVDMFIRPVTGFLGNTKLVRPKIQYFFSFAVSYNGICHLLCPLLPGYAGLVGYAVILGLGFGMVSALLFEVLMDLVGAHRFSSAVGLVTIIECGPVLLGPPISGALVDAYGDYKYMYYACGVFMLAPGIFLFVMHYFNYKKLEEEQRQSVAVEMRTSEETVQLNMSQSDKSTNKAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIF7</accession>
    <name>A0A672YIF7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Intercellular adhesion molecule 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115421316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030137182.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSSORT00005004449.1">
        <property type="protein sequence ID" value="ENSSORP00005004318.1"/>
        <property type="gene ID" value="ENSSORG00005002623.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421316"/>
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    <dbReference type="Proteomes" id="UP000472271">
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    </dbReference>
    <dbReference type="GO" id="GO:0005178">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="3"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13771:SF9">
        <property type="entry name" value="INTERCELLULAR ADHESION MOLECULE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03921">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025401046" evidence="1">
        <location>
            <begin position="23"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="226"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="376" mass="41459" checksum="AE3889DFE0AB116B" modified="2020-06-17" version="1">MSPCVFLFLICVNSLGVSHVRSSDDPEANTCEDPPVFSPSHLVVKHGDPTTATCTVCKEKCHNKHMGLEKPLGTDSMNGTTITWTVDKMTEWDTFVMCYYNTESRQCSSSLPITVYQPPVRVSFNFVNHSGPMILGHTYIMQCSVEDVAPVGGLTVVFYKGETELNETKLDGEEKNPVTIAVLFQYHPVRGYHNWNYYCKATLQLGTEHFSWKSSEEVNIDTYFPPQLDVPPYPNPINVTEGDALVLNCSAYGSPRTHYRWKLPTGQTIAGNSIFTLDPVSVSDEGHYICSASNNLGTDTVDYSVFVQRVTTTTTATTTTAATTTATTTTATTTQRVLDTTSIVVTTRSTRSTGSTSRTPIHRFTLGFMLLFLASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJA5</accession>
    <name>A0A672YJA5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJA5"/>
    <dbReference type="Ensembl" id="ENSSORT00005004792.1">
        <property type="protein sequence ID" value="ENSSORP00005004656.1"/>
        <property type="gene ID" value="ENSSORG00005002837.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJA5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002198">
        <property type="entry name" value="Immunoglobulin heavy variable 5-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="6"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="141" mass="16004" checksum="E894EEA2CFD556F4" modified="2020-06-17" version="1">MRWSLPHTLPTTLCVDGQTLIESGSAVKSPGESHRLTCTTSGFTFSSHWMHWVRQAPGIGLEWIATVDTGDSSKYYSQSVQGRFTISRDNGRNQLYLQMNNLKTEDSAVYYCARDPQCLELVKQLYKILKNDSVVVILYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YK71</accession>
    <name>A0A672YK71_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ksr2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A672YK71"/>
    <dbReference type="Ensembl" id="ENSSORT00005005052.1">
        <property type="protein sequence ID" value="ENSSORP00005004912.1"/>
        <property type="gene ID" value="ENSSORG00005002957.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YK71"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000107">
        <property type="entry name" value="kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000031">
        <property type="entry name" value="Kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000010">
        <property type="entry name" value="Putative kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025561">
        <property type="entry name" value="KSR_SAM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046861">
        <property type="entry name" value="SAM_KSR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046933">
        <property type="entry name" value="SAM_KSR1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF775">
        <property type="entry name" value="KINASE SUPPRESSOR OF RAS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13543">
        <property type="entry name" value="SAM_KSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20406">
        <property type="entry name" value="SAM_KSR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="373"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="594"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="211"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="455"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="470"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="881" mass="99635" checksum="BE52033E244C000A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKG5</accession>
    <name>A0A672YKG5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chloride channel 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YKG5"/>
    <dbReference type="Ensembl" id="ENSSORT00005005209.1">
        <property type="protein sequence ID" value="ENSSORP00005005064.1"/>
        <property type="gene ID" value="ENSSORG00005003035.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YKG5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062158">
        <property type="term" value="F:chloride:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002249">
        <property type="entry name" value="CIC-7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051280">
        <property type="entry name" value="Cl-channel/antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689:SF136">
        <property type="entry name" value="H(+)_CL(-) EXCHANGE TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01118">
        <property type="entry name" value="CLCHANNEL7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="309" mass="34022" checksum="B7B32D65DD966389" modified="2020-06-17" version="1">IPHNEKLLSLKYESLDYDNIENQLFLEEERRMSHMGFRCLEISRWVVCGLIGFLTGLIACFIDIVVEQLAGIKYQVVKENIERFTEFGGLSISLILWAVLNSTFVMVGAIIVAFFEPIAAGSGIPQIKCYLNGVKIPRVVRLKTLVVKVCGVICSVVGGLAVGKEGPMIHSGAVVAAGVSQGRSTSLKRDFKIFEYFRRDTEKRDFVSAGAAAGVSAAFGAPVGGVLFSLEEGASFWNQMLTWRIFFASMISTFTLNFFLSIYHKNPGDLSNPGLINFGRFESESMAYNLYEIPLFIAMGLLCSCLICA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YL51</accession>
    <name>A0A672YL51_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YL51"/>
    <dbReference type="Ensembl" id="ENSSORT00005005477.1">
        <property type="protein sequence ID" value="ENSSORP00005005321.1"/>
        <property type="gene ID" value="ENSSORG00005003166.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YL51"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034113">
        <property type="term" value="P:heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150079">
        <property type="term" value="P:negative regulation of neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047164">
        <property type="entry name" value="OX2G-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841:SF7">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841">
        <property type="entry name" value="OX-2 MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="121" mass="13893" checksum="EB5E0A894E3708E0" modified="2020-06-17" version="1">MKSEDVVQVTWQKISPERNLATNSKYFNPRVNPAFRDKVEFKDAGLQNSTLIIRNVNQQDEGCYNCLFNIQHQGAITCKTCLQIYGKNLPASLNVCETLQNQILVQSTFHDFILNIQQLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLD7</accession>
    <name>A0A672YLD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane 4 L6 family member 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tm4sf21b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the L6 tetraspanin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLD7"/>
    <dbReference type="Ensembl" id="ENSSORT00005005573.1">
        <property type="protein sequence ID" value="ENSSORP00005005415.1"/>
        <property type="gene ID" value="ENSSORG00005003218.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLD7"/>
    <dbReference type="OrthoDB" id="9937421at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008661">
        <property type="entry name" value="L6_membrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198:SF23">
        <property type="entry name" value="SI:CH211-137I24.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05805">
        <property type="entry name" value="L6_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="199" mass="20953" checksum="F995FF75724B9EF5" modified="2020-06-17" version="1">MCTGACSKVIAVCLYVLAALSVICNIMLFFPDFSTEYAATDRGGENRITAEVKYMGGLIGGGIMVLIPAIHIHLTSAKNCCANRCGMFLSIGFAAAGVVGAVYSLSVAALGLANGPLCYWSNADNIIPQWGTPFFNSTGSYLGDKDMWKWCKIPENVVGFNVGLFSTLLVASCLELVLCAIQMVNGLFGCLCGTCGGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLQ2</accession>
    <name>A0A672YLQ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Methionine sulfoxide reductase B1</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Acts as a regulator of actin assembly by reducing methionine (R)-sulfoxide mediated by MICALs (MICAL1, MICAL2 or MICAL3) on actin, thereby promoting filament repolymerization. Plays a role in innate immunity by reducing oxidized actin, leading to actin repolymerization in macrophages.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (R)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:21260"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58773"/>
            <dbReference type="EC" id="1.8.4.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLQ2"/>
    <dbReference type="FunCoup" id="A0A672YLQ2">
        <property type="interactions" value="445"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005690.1">
        <property type="protein sequence ID" value="ENSSORP00005005504.1"/>
        <property type="gene ID" value="ENSSORG00005003287.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLQ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033745">
        <property type="term" value="F:L-methionine-(R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052150">
        <property type="entry name" value="MsrB_Met_sulfoxide_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46755">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46755:SF5">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="17">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="92" mass="10284" checksum="3A4883DDCA80AE18" modified="2020-06-17" version="1">MSYCSFTGGEEYKNHFQPGIYVCSECGHELFSSTSKFEHSSPWPAFSQTIHEDSVSKHPEAWGPIKVCCGKCGNGLGHEFMYDGPREGLSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMF4</accession>
    <name>A0A672YMF4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Monocyte to macrophage differentiation factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115423670</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YMF4"/>
    <dbReference type="Ensembl" id="ENSSORT00005006022.1">
        <property type="protein sequence ID" value="ENSSORP00005005775.1"/>
        <property type="gene ID" value="ENSSORG00005003460.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YMF4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140911">
        <property type="term" value="F:pore-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005744">
        <property type="entry name" value="Hy-lIII"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01065">
        <property type="entry name" value="hlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF26">
        <property type="entry name" value="MONOCYTE TO MACROPHAGE DIFFERENTIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="244" mass="28007" checksum="7D3952C80D3584EE" modified="2020-06-17" version="1">TLGLCLCLCRRFMNRRASANCRYQPTCYEHAANCYTHAFLIAPAFVGMALLHRLSDNRWETITAWVYGIGLCALFLVSTVFHIITWKKSHMRAVEHCFHMCDRVVIYFFIAASYTPWLNLRELGPLAAHMRWFVWLMAAAGTIYVFNYHEKYKLVELAFYLTMGFFPASVVTSMSNTEGLHELACGGLIYCLGVVFFKSDGIIPFAHAIWHVFVALAAAVHYYAIWKYLYRNRGDSDHALLLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMM2</accession>
    <name>A0A672YMM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Jupiter microtubule associated homolog 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">jpt2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JUPITER family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997846.1">
        <property type="nucleotide sequence ID" value="XM_030141986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YMM2"/>
    <dbReference type="FunCoup" id="A0A672YMM2">
        <property type="interactions" value="1003"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006087.1">
        <property type="protein sequence ID" value="ENSSORP00005005840.1"/>
        <property type="gene ID" value="ENSSORG00005003510.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424618"/>
    <dbReference type="CTD" id="90861"/>
    <dbReference type="InParanoid" id="A0A672YMM2"/>
    <dbReference type="OrthoDB" id="6367565at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930">
        <property type="entry name" value="GEO05313P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34930:SF5">
        <property type="entry name" value="JUPITER MICROTUBULE ASSOCIATED HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17054">
        <property type="entry name" value="JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="22007" checksum="1302F54307FF14E5" modified="2020-06-17" version="1">MTSTNMFQGLDTGSKPSSRVLRPPGGGSSNLFGGYEDDSASRRPNRMASTVFAPAEEPQSVPKRSNPPGGKSSGIFAEAEAPAQPQKPMAPVGQTSNIFGAGESAPAPSPSRNHPNKPKDNLSVGPEPESPAPAVKVSQPEVKVESPAPAVAPVKEEPPAPAAAAAPPPELQPASSLATDEASLKNHEPHLGPKPRSHNRVLNPPGGKSSVVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMR2</accession>
    <name>A0A672YMR2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Hepatic leukemia factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hlfa</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. PAR subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YMR2"/>
    <dbReference type="FunCoup" id="A0A672YMR2">
        <property type="interactions" value="603"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006070.1">
        <property type="protein sequence ID" value="ENSSORP00005005823.1"/>
        <property type="gene ID" value="ENSSORG00005003502.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YMR2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000007">
        <property type="entry name" value="hepatic leukemia factor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF28">
        <property type="entry name" value="HEPATIC LEUKEMIA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="230"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="94"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="300" mass="33457" checksum="AF77F7CE6E6A7F9A" modified="2020-06-17" version="1">MEKMARPLPINTTFLPPTHGVLKSLLENPLKLPFHHDEGFSKDKEKEKKLEDESSAASHPQSAFLGPTLWDKTLPYDGDNFQLEYMDLEEFLSENGIPANTAQSDQNPQAVAAQPPQAPPPAPPTPSVVDLSSRATTSVHTTMAPQTCLTTRPGGRGPLPSSRDTPSPIDPESIQVPVTYDPDPADLALSSVPGQEMFDPRKRKFSAEELKPQPMIKKARKVFIPEDLKDDKYWARRRKNNVAAKRSRDARRLKENQIAIRAGFLEKENAALRMEVADLRKELGRCKNIVAKYEARHGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNL3</accession>
    <name>A0A672YNL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115423137</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YNL3"/>
    <dbReference type="Ensembl" id="ENSSORT00005006463.1">
        <property type="protein sequence ID" value="ENSSORP00005006211.1"/>
        <property type="gene ID" value="ENSSORG00005003691.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YNL3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046642">
        <property type="term" value="P:negative regulation of alpha-beta T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002720">
        <property type="term" value="P:positive regulation of cytokine production involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000406">
        <property type="term" value="P:positive regulation of T cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13405">
        <property type="entry name" value="TNFRSF14_teleost"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000007">
        <property type="entry name" value="TNF receptor superfamily member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46838">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46838:SF1">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="67"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="67"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="250" mass="27129" checksum="918ADBA03D6679EF" modified="2020-06-17" version="1">MLNEKNKHKLDLLFLFFSGYVAVLSLPALCCRPKEYSTAEGQCCPMCHEGTVVRKDCTSQSGTRCIPCDDGTFMNKPNGLASCFHCSACDSVRGLLVQQTCTYTTDTVCGVLDGYFCKSFTEDSGCDSAQEHKPCAAGQRIKEPGTSRTDTVCEDCPQGFFSKHGVNCTPWTVCTETQVKTKEGSSAEDVVCAASSRIHTFLIIPCVLLGLTTVLTHSLITLFFSITVGDVPSYNECLKHSTSGEEIFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YP78</accession>
    <name>A0A672YP78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="3">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">amhr2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672YP78"/>
    <dbReference type="Ensembl" id="ENSSORT00005006570.1">
        <property type="protein sequence ID" value="ENSSORP00005006317.1"/>
        <property type="gene ID" value="ENSSORG00005003468.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YP78"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005024">
        <property type="term" value="F:transforming growth factor beta receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23616">
        <property type="entry name" value="TFP_LU_ECD_AMHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF49">
        <property type="entry name" value="ANTI-MUELLERIAN HORMONE TYPE-2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="190"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="505" mass="56600" checksum="B3D155C01B6ABD31" modified="2020-06-17" version="1">MCNNYSSCLSLAEFIFISISSQSLLQKRQCLFHVKTQNDKYRTAGNVSGSVQFCEKTDCCVGYFLINGSQPEVDLLACDMAEKSCPDSTCKAQIRFSNHLIKCVCNTDLCNSNITWSVEPAQSEFTYTYSVGDKIKIVVIVFALLILCGIAAKWKTVLKQTKDPQTSFHSVAPLCSCLMTKTPDIDVANVELQQIVGEGYYATVWQGKYQGNKVAVKVFPAAWKQKFANEKVVYELPLMNHAGIVNFLGSGRKSDGSSWLIVLQFAEYGSLHSFLGNHTSSWMLSLTLCQSLSQGLAYLHSDLHRHDVHKPPVAHRDLSSANVLVRADGTCALSDFGCSTVLRSCLGHHCRQNHTRNTEVHPQVGTPRYMAPEILDGSVNLKSSLCLIQGDVYALGLLMWEIWMRCSDLFNGETVPQHLLPYESELEANVTLENLILYVFYMDKRPSIPKLWELQPQGSELQKLLTDCWDYDPDARLTAQCVADRLGSFQSYCSLFYRHLSCFNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTJ2</accession>
    <name>A0A672YTJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Family with sequence similarity 91 member A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fam91a1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM91 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030003366.1">
        <property type="nucleotide sequence ID" value="XM_030147506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YTJ2"/>
    <dbReference type="FunCoup" id="A0A672YTJ2">
        <property type="interactions" value="1888"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008219.1">
        <property type="protein sequence ID" value="ENSSORP00005007936.1"/>
        <property type="gene ID" value="ENSSORG00005004451.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428454"/>
    <dbReference type="CTD" id="157769"/>
    <dbReference type="InParanoid" id="A0A672YTJ2"/>
    <dbReference type="OrthoDB" id="275996at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099041">
        <property type="term" value="P:vesicle tethering to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039199">
        <property type="entry name" value="FAM91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028097">
        <property type="entry name" value="FAM91_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028091">
        <property type="entry name" value="FAM91_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28441:SF2">
        <property type="entry name" value="PROTEIN FAM91A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14648">
        <property type="entry name" value="FAM91_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14647">
        <property type="entry name" value="FAM91_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM91 N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="FAM91 C-terminal" evidence="4">
        <location>
            <begin position="373"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="664"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="773"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="814"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="666"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="773"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="833" mass="93813" checksum="336EAD3ED6878538" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YU07</accession>
    <name>A0A672YU07_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">camk2b1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672YU07"/>
    <dbReference type="Ensembl" id="ENSSORT00005008255.1">
        <property type="protein sequence ID" value="ENSSORP00005007972.1"/>
        <property type="gene ID" value="ENSSORG00005004464.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YU07"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000001">
        <property type="entry name" value="calcium/calmodulin-dependent protein kinase type II subunit gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="13"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="333"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="506" mass="56366" checksum="5EB87EBE0573A773" modified="2020-06-17" version="1">MATTTCTRFTDEYQLYEELGKGAFSVVRRCVKLCTGQEYAAKIINTKKLSARGKPMFMLLKMRTIRLHDSISEEGFHYLLFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKNAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAQEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKVCFHMLGSRQTTAPASVTAAAAAVAAAAGTTAGLVEQGSMVPQTNSTKNSIVTSPKGNIPSPALESSDSSNTTVEDEDVKARKQEIIKITEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHLIGEDAACIAYIRLTQFVDGQGRPRSSQSEETRVWHRRDSKWQNIHFHCSGAPAAPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUA8</accession>
    <name>A0A672YUA8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Pyridoxal (pyridoxine, vitamin B6) phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pdxp</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029998952.1">
        <property type="nucleotide sequence ID" value="XM_030143092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YUA8"/>
    <dbReference type="FunCoup" id="A0A672YUA8">
        <property type="interactions" value="954"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008454.1">
        <property type="protein sequence ID" value="ENSSORP00005008168.1"/>
        <property type="gene ID" value="ENSSORG00005004558.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425481"/>
    <dbReference type="CTD" id="57026"/>
    <dbReference type="InParanoid" id="A0A672YUA8"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07510">
        <property type="entry name" value="HAD_Pase_UmpH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000163">
        <property type="entry name" value="Pyridoxal phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF94">
        <property type="entry name" value="CHRONOPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34587" checksum="73F48C202BDDBDEA" modified="2020-06-17" version="1">MAGAFGFKGCQKIRGPQIRNLLEAKDFFLFDCDGVIWHGEKAITGAAKVVNSLIRHGKNVVFVTNNCTRPRENYVHKFYRLGFTDVMLEQIFSSSYCSALYLRDVVKICGQVFVIGCDGLRRELQEAGIPCVEEADDPDATIYNCALAPDVKAVLVGHDDKLTFLKLAKASCYLRDPDCLFLATDNDPWHPLSGGRILPGSGSLTAALEVASGRKATVIGKPSRFMFECISSQFRGVDPAQCLMVGDRLETDMLFGSNCGLDTMLTLTGVSQIEEAQEYRNSELTTNHNLVPDYVVDTIADFLPAFEDLDEEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYW5</accession>
    <name>A0A672YYW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear receptor 2C2-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nr2c2ap</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NR2C2AP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987659.1">
        <property type="nucleotide sequence ID" value="XM_030131799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YYW5"/>
    <dbReference type="FunCoup" id="A0A672YYW5">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010025.1">
        <property type="protein sequence ID" value="ENSSORP00005009699.1"/>
        <property type="gene ID" value="ENSSORG00005005309.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417754"/>
    <dbReference type="CTD" id="126382"/>
    <dbReference type="InParanoid" id="A0A672YYW5"/>
    <dbReference type="OrthoDB" id="10052260at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000070">
        <property type="entry name" value="Nuclear receptor 2C2-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="141" mass="15771" checksum="E8480944E98D7555" modified="2020-06-17" version="1">MASSLICGETQSRVSSVLNRDVKQFGKKYMFDSSEETCWNSDQGECQWVSLEFPQSVRVSELKVQFQGGFSAKTCRIEGCPKGGDFAEICLFYPEDNNSLQSFLIQEASAVDKVKIMFENSADFFGRIIVYSLDVLGEKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0T4</accession>
    <name>A0A672Z0T4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Blue-sensitive opsin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115419153</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z0T4"/>
    <dbReference type="FunCoup" id="A0A672Z0T4">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010802.1">
        <property type="protein sequence ID" value="ENSSORP00005010465.1"/>
        <property type="gene ID" value="ENSSORG00005005549.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0T4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000018">
        <property type="entry name" value="Rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001521">
        <property type="entry name" value="Opsin_blue"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00574">
        <property type="entry name" value="OPSINBLUE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0157">Chromophore</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 17" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="5 17" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="15 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="60"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="352" mass="38853" checksum="0E9D472D32C9429E" modified="2020-06-17" version="1">MRSNRPVEFPDDFWIPIPLDTDNVTSLSPYLVPQDHLGSPGIFYSLSAFMFFLFVTGTGINTLTIACTAQYKKLRSHLNYILVNLAVANLLVSSVGSFTCFYCFAFKYMVLGPLGCKIEGFTATVGGMVSLWSLAVIAFERWLVICKPLGNFVFRPNHAVGCCALTWVFALAAAVPPLVGWSRYIPEGMQCSCGPDWYTVNNKFNNESYVMFLFGFCFAIPFSTIVFCYSQLLFTLKSAAKAQAESASTQKAEREVTRMVVVMVLGFLVCWMPYASFALWVVNNRGQPFDLRLATIPSCLSKASTVYNPVIYILLNKQFCSCLKKMLGMSSSDDDESSAASSATEVSKVGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0U7</accession>
    <name>A0A672Z0U7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neurexophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be signaling molecules that resemble neuropeptides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the neurexophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z0U7"/>
    <dbReference type="Ensembl" id="ENSSORT00005010708.1">
        <property type="protein sequence ID" value="ENSSORP00005010373.1"/>
        <property type="gene ID" value="ENSSORG00005005604.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0U7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010450">
        <property type="entry name" value="Nxph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103">
        <property type="entry name" value="NEUREXOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103:SF13">
        <property type="entry name" value="NEUREXOPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038019">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="32389" checksum="0C0E134896DF469D" modified="2020-06-17" version="1">MHDARSNTINIECSSEETRIKRQKIADLPAPGSSDLRESPKSKVKTYWTESSKAFSISRLLSQSLYKENFTSLDLNYDDADSYSKQEQWNWLYNSSNPRDPRSRTKRRPIVKTGKFKKMFGWGDFHSNIKTVKLNLLITGKIVDHGNGTFSVYFRHNSTGQGNVSVGLVPPTKAVEFQVHQQYHHHNQQQQTALETKDTKLFNCRVEYEKVEKGTRNSLCAHDPSQSCPQEQTQSHVSWLCSKPFKVICIFITFYSTDYKLVQKVCPDYNYHSDTPYLPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z109</accession>
    <name>A0A672Z109_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z109"/>
    <dbReference type="Ensembl" id="ENSSORT00005010878.1">
        <property type="protein sequence ID" value="ENSSORP00005010540.1"/>
        <property type="gene ID" value="ENSSORG00005005703.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z109"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039751">
        <property type="entry name" value="HERPUD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943:SF5">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UBIQUITIN-LIKE DOMAIN MEMBER 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UNIQUITIN-LIKE DOMAIN HERPUD PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="74" mass="8402" checksum="4EA1CF742FDFB6A9" modified="2020-06-17" version="1">MDSGAVDSPVTLVIKAPNQKYEDQTINCFLNWTVERLKSHISNVYPSKPVCDLTFSVACFWLLVVFTDSILKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6Z8</accession>
    <name>A0A672Z6Z8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Latent transforming growth factor beta binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ltbp3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the LTBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030010615.1">
        <property type="nucleotide sequence ID" value="XM_030154755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z6Z8"/>
    <dbReference type="FunCoup" id="A0A672Z6Z8">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013022.1">
        <property type="protein sequence ID" value="ENSSORP00005012625.1"/>
        <property type="gene ID" value="ENSSORG00005006538.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433380"/>
    <dbReference type="CTD" id="4054"/>
    <dbReference type="InParanoid" id="A0A672Z6Z8"/>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000068">
        <property type="entry name" value="Latent transforming growth factor beta binding protein 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000019">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000077">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000056">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000017">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000115">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000160">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050751">
        <property type="entry name" value="ECM_structural_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034:SF46">
        <property type="entry name" value="LATENT-TRANSFORMING GROWTH FACTOR BETA-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025557922" description="Latent transforming growth factor beta binding protein 3" evidence="13">
        <location>
            <begin position="22"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="15">
        <location>
            <begin position="217"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="293"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="15">
        <location>
            <begin position="338"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="473"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="515"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="559"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="642"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="683"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="724"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="764"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="15">
        <location>
            <begin position="816"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="934"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="980"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="1027"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="15">
        <location>
            <begin position="1077"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="1197"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="416"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1246" mass="138105" checksum="E36A196B3CECAF37" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7K0</accession>
    <name>A0A672Z7K0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Melanoregulin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mreg</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z7K0"/>
    <dbReference type="FunCoup" id="A0A672Z7K0">
        <property type="interactions" value="730"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013227.1">
        <property type="protein sequence ID" value="ENSSORP00005012825.1"/>
        <property type="gene ID" value="ENSSORG00005006702.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z7K0"/>
    <dbReference type="OrthoDB" id="10015106at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032402">
        <property type="term" value="P:melanosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031638">
        <property type="entry name" value="Melanoregulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34340">
        <property type="entry name" value="MELANOREGULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34340:SF1">
        <property type="entry name" value="MELANOREGULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15812">
        <property type="entry name" value="MREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="238" mass="27201" checksum="886CC451A58C44AF" modified="2020-06-17" version="1">MGSGFRKFCLQFCCCCCARDEDDEDEKQPLVPQDPLEYFNREVQKRRDEETNLWSTPGDPSHSERDDDRTLYTLIQARNKTRMGSTGYRRLSVDIEAMRDTRREVRDKWKTILENLGFMAEADSLLTVSAGASHDRMRNAPAARAMLTALHSETSIFNSKEPPPERYLFILDRLIYLDIAEDFMAKARRFYPPRDDSDEEMSPLGINLPLLLARVEAMNGGGDEDDDSDASGNLSDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z807</accession>
    <name>A0A672Z807_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lactadherin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115417126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029986982.1">
        <property type="nucleotide sequence ID" value="XM_030131122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z807"/>
    <dbReference type="FunCoup" id="A0A672Z807">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013402.1">
        <property type="protein sequence ID" value="ENSSORP00005012994.1"/>
        <property type="gene ID" value="ENSSORG00005006504.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417126"/>
    <dbReference type="InParanoid" id="A0A672Z807"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000226">
        <property type="entry name" value="EGF-like repeat and discoidin I-like domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF7">
        <property type="entry name" value="COAGULATION FACTOR VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025596357" evidence="3">
        <location>
            <begin position="28"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="26"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="67"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="113"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
            <begin position="152"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
            <begin position="319"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="478" mass="52700" checksum="4AC626B8CC93736F" modified="2020-06-17" version="1">MNRHGNGLIAPTLTALTVLVSLSSVTGNYCEVNLCHNGATCVTGVGEEPFICICPDGFGGDTCNLTDTGPCSPNPCKNDGTCELLTPTRRGDVFNEYICRCQPGFEGVHCQINVNDCDNEPCANGGKCRDLDGDYTCQCPSPYVGKQCQLRCISLLGMEGGAIVESQISASSVHYGILGLQRWGPELARLNNQGIVNAWTSASHDRNPWIEINMQKKMRLTGIITQGASRMGASEYIKAFKVASSFDGVTYTTHRADGQRRDKVFVGNTDNDSTKTNMFDPPIVAQFIRIIPVVCRRACTLRMELVGCELNVYSNTAGCSEPLGMKSRLISDGQLSASSTFRTWGIDTFTWHPQFARLDKQGKTNAWSPANNNRSEWIQVDLEKTKRLTGIITQGAKDFGVVQFVSVFKVMYSNNGESWSTVKDGNTGNDKLFQGNTDNNTHKKNLFEPPFYAQFVRVVPLEWHERITLRMELLGCDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z856</accession>
    <name>A0A672Z856_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS7m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S7, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mrps7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029996404.1">
        <property type="nucleotide sequence ID" value="XM_030140544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z856"/>
    <dbReference type="FunCoup" id="A0A672Z856">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013452.1">
        <property type="protein sequence ID" value="ENSSORP00005013044.1"/>
        <property type="gene ID" value="ENSSORG00005006796.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423633"/>
    <dbReference type="CTD" id="51081"/>
    <dbReference type="InParanoid" id="A0A672Z856"/>
    <dbReference type="OrthoDB" id="9972728at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14870">
        <property type="entry name" value="uS7_Mitochondria_Mammalian"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000004">
        <property type="entry name" value="28S ribosomal protein S7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="9">
        <location>
            <begin position="69"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="229" mass="26694" checksum="0C7FC37CAF29DCF5" modified="2020-06-17" version="1">MAASISGLLKPWIPRVFLVRWSRYNPYYLEPEVRKEVYNRPETELSAEEKEQQELKEMRSIKAATGGITSSVFSDPSISKFTNMMMKHGNKALAREIISVTLETVKRKQVEKYHKAPAGKKDEIECNPYVIFHQALENCKPVVGLISVPKGGRFYQVPVPLSENRRRFLAMKWMITECRDNKHRTTPMHEKLSQELLAAFIKEGNVVKKRNEMHKVAEANRAYAHYRWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z940</accession>
    <name>A0A672Z940_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytoplasmic phosphatidylinositol transfer protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Retinal degeneration B homolog beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115423689</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidic acid (PA) between membranes. Binds PA derived from the phospholipase D signaling pathway and among the cellular PA species, preferably binds to the C16:0/16:1 and C16:1/18:1 PA species.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate(in) = a 1,2-diacyl-sn-glycero-3-phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:36435"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:36436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PtdIns transfer protein family. PI transfer class IIB subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z940"/>
    <dbReference type="Ensembl" id="ENSSORT00005013446.1">
        <property type="protein sequence ID" value="ENSSORP00005013038.1"/>
        <property type="gene ID" value="ENSSORG00005006793.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z940"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000011">
        <property type="entry name" value="cytoplasmic phosphatidylinositol transfer protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF55">
        <property type="entry name" value="CYTOPLASMIC PHOSPHATIDYLINOSITOL TRANSFER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="250"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="318" mass="36835" checksum="59420B8D10744322" modified="2020-06-17" version="1">YRIGQLYMISKHSHEQSERGEGVEVVQNEPYEDPEHGQGQFTEKRIYLNNKLPSWARAVVPKIFYVTEKAWNYYPYTITVLIASFLLQCSFLPKFSIHIETKYEDNKGSNDNIFGSEPRDEETEVCAVDIAYDEIPERHYKESEDLRYFKSKKTDRGLLQEGWIDTQDPIMCSYKLVTVKFEVWGLQTRVEQFVHKVIRDVLLLGHRQAFAWVDEWIDMTMEEVREYERATQEATNLKIGTFPPAISITETPLPSVTRSGPNSAPSTPLSTEAPEFLTVPKERPRKKSAPETLTLPDPEKQPDLHNKSCRCQQCSVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZC52</accession>
    <name>A0A672ZC52_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZC52"/>
    <dbReference type="Ensembl" id="ENSSORT00005014497.1">
        <property type="protein sequence ID" value="ENSSORP00005014076.1"/>
        <property type="gene ID" value="ENSSORG00005007214.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014508.1">
        <property type="protein sequence ID" value="ENSSORP00005014087.1"/>
        <property type="gene ID" value="ENSSORG00005007221.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="61" mass="6891" checksum="45B34E42E849A95E" modified="2020-06-17" version="1">MADEEAEKDRGPENGISATIGELKQRLQELQDVVFKERNECPVQASLNYCQEFCRVMAQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZC76</accession>
    <name>A0A672ZC76_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZC76"/>
    <dbReference type="Ensembl" id="ENSSORT00005014867.1">
        <property type="protein sequence ID" value="ENSSORP00005014444.1"/>
        <property type="gene ID" value="ENSSORG00005007395.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017177.1">
        <property type="protein sequence ID" value="ENSSORP00005016667.1"/>
        <property type="gene ID" value="ENSSORG00005008430.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="61" mass="7216" checksum="15B8C6826BC81751" modified="2020-06-17" version="1">GFAEQCVDLTCKRILDAARGKTRINIGSAFERWRRLKQPHLELKSDEMITEFLLDRYFTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZD84</accession>
    <name>A0A672ZD84_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Odorant receptor 131-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115419945</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZD84"/>
    <dbReference type="Ensembl" id="ENSSORT00005015792.1">
        <property type="protein sequence ID" value="ENSSORP00005015301.1"/>
        <property type="gene ID" value="ENSSORG00005007771.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZD84"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF998">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="38"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="35761" checksum="C2D2112A0877EB30" modified="2020-06-17" version="1">MNVSSSNVTVVIQYRDSFSKAVTKNVIVVVLGISINYINASLIRTFRKHQVFYTNPRYILFCHLVVNDMIQVTLTVMLFLLSYILYKINVSLCCLFILLALFTTENTPLNLACMAVECYIAICIPLRHTQICTVRRTLQLIGLIWLTSMLSVLPDLFISLATESREFFASQVFCLRETAFRNPHIIKKRNVTYIVYLVLVWLVIFFTYFKILFTAKTASKDTRKARNTIILHGFQLLLCMATYAVHLLKTALMEWFPKNYPDSLFACYIIIQMLPRSISPLIYGVRDNTTFRKYFKKYLLCSNQVNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDH7</accession>
    <name>A0A672ZDH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mos1 transposase HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZDH7"/>
    <dbReference type="Ensembl" id="ENSSORT00005014942.1">
        <property type="protein sequence ID" value="ENSSORP00005014517.1"/>
        <property type="gene ID" value="ENSSORG00005007428.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDH7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041426">
        <property type="entry name" value="Mos1_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052709">
        <property type="entry name" value="Transposase-MT_Hybrid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001888">
        <property type="entry name" value="Transposase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46060">
        <property type="entry name" value="MARINER MOS1 TRANSPOSASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46060:SF1">
        <property type="entry name" value="MARINER MOS1 TRANSPOSASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17906">
        <property type="entry name" value="HTH_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01359">
        <property type="entry name" value="Transposase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mos1 transposase HTH" evidence="2">
        <location>
            <begin position="13"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="312" mass="35854" checksum="85044276A314E63A" modified="2020-06-17" version="1">MGSRPQRLKTLRYELRAVIKYLHKKGMTPKEIHEDMVSTLGKECLAYSTVRKWVAEFKQGRTSTEDDPRSGCPKTSSSDEQVDAIHCMVLSDRRMTVQQIAKATDISVGSVHHVLTDILGMNKLSARWVPRMLTPEQMLKRVDISRTLLTRFHADPENFQRRIVTQDETWVHHFEPESKLQILMVDYLQKGQTINGEFYASELRKLKKEIKAKRRGKLRAGVLLLQDNAPVHTAQVAVAEAHNCGFELLPHPPYSPDLSHLCGRHFRNDDDVIHAVEEFLGDQDVTFFRDGIVMLEHRWTKCIAVNGDYVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZED1</accession>
    <name>A0A672ZED1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Neurofilament light</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZED1"/>
    <dbReference type="Ensembl" id="ENSSORT00005015655.1">
        <property type="protein sequence ID" value="ENSSORP00005015184.1"/>
        <property type="gene ID" value="ENSSORG00005007690.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZED1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099160">
        <property type="term" value="C:postsynaptic intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099184">
        <property type="term" value="F:structural constituent of postsynaptic intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033693">
        <property type="term" value="P:neurofilament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006821">
        <property type="entry name" value="Intermed_filament_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF16">
        <property type="entry name" value="NEUROFILAMENT LIGHT POLYPEPTIDE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04732">
        <property type="entry name" value="Filament_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="89"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="443"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="93"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="308"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="456"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="536" mass="60081" checksum="A4667C9628AAC1E0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZEP5</accession>
    <name>A0A672ZEP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metalloprotease TIKI</fullName>
            <ecNumber evidence="13">3.4.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">TRAB domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">trabd2a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the N-terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="13">Divalent metal cations. Mn(2+) or Co(2+).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TIKI family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_030005337.1">
        <property type="nucleotide sequence ID" value="XM_030149477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZEP5"/>
    <dbReference type="Ensembl" id="ENSSORT00005015780.1">
        <property type="protein sequence ID" value="ENSSORP00005015289.1"/>
        <property type="gene ID" value="ENSSORG00005007761.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429764"/>
    <dbReference type="CTD" id="129293"/>
    <dbReference type="InParanoid" id="A0A672ZEP5"/>
    <dbReference type="OrthoDB" id="10040378at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14789">
        <property type="entry name" value="Tiki"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040230">
        <property type="entry name" value="TIKI1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31120">
        <property type="entry name" value="METALLOPROTEASE TIKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31120:SF7">
        <property type="entry name" value="METALLOPROTEASE TIKI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025453649" description="Metalloprotease TIKI" evidence="15">
        <location>
            <begin position="20"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="350"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="518" mass="60233" checksum="770B5F956487375D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFZ1</accession>
    <name>A0A672ZFZ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Vertebrate ancient opsin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tmtops2b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZFZ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005016437.1">
        <property type="protein sequence ID" value="ENSSORP00005015940.1"/>
        <property type="gene ID" value="ENSSORG00005008059.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZFZ1"/>
    <dbReference type="OrthoDB" id="2101615at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15086">
        <property type="entry name" value="7tmA_tmt_opsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000197">
        <property type="entry name" value="Teleost multiple tissue opsin 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002962">
        <property type="entry name" value="Peropsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01244">
        <property type="entry name" value="PEROPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 14" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="56"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="380" mass="42349" checksum="6773CA532EE9869B" modified="2020-06-17" version="1">MDLRMLSGQAALNYSFNLTEDPEQVDTPTVPETLSPTGFVVLSVFLGFIMTFGFLNNFIVLILFCKFKKLRTPVNMLLLNISVSDMLVCLFGTTLSFASSIRGRWLLGRSGCSWYGFINSCFGIVSLISLVILSYDRYSTLTVYNKRGPSYRKPLLAVGGSWLYSLFWTVPPLLGWSSYDTEGAGTSCSVSWTLRSAQSHAYIICLFIFCLGLPVLVMIYCYSRLLLAVKQVGKIRKTAARRREYHILFMVLTTAACYLLCWMPYGVVAMMATFGPPNIISPVASVVPSLLAKSSTVINPLIYILMNKQFYRCFLVLFHCNHWSAENGNTSMPSKTTVIQLNRRVYSNTVACTAQISNATLNQFSSTPASKRTNPPEATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJB9</accession>
    <name>A0A672ZJB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgin A3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">golga3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029998594.1">
        <property type="nucleotide sequence ID" value="XM_030142734.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029998595.1">
        <property type="nucleotide sequence ID" value="XM_030142735.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A672ZJB9">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017387.1">
        <property type="protein sequence ID" value="ENSSORP00005016874.1"/>
        <property type="gene ID" value="ENSSORG00005003989.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425297"/>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 3"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tropomyosin"/>
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        <location>
            <begin position="1458"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
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    <sequence length="1531" mass="172768" checksum="F25A477AA1644929" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJR2</accession>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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    <reference evidence="6" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
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        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
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    </comment>
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        <text evidence="3">Belongs to the GASK family.</text>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00007691"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="130" mass="15154" checksum="56C3FD28875DB179" modified="2020-06-17" version="1">MCVWSRTTTLNVGTGTTSTWPTLSTEATHPRHLVFTDNKGFFDRNEDNLDFRLLEGIKELPEQAVSVLRSRRLREKLLQSLFLDQTYWESQGGRQGIDKLIDVIERRAKVLLTYINAHGILVTFRERKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKE5</accession>
    <name>A0A672ZKE5_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">5-hydroxytryptamine receptor 7</fullName>
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            <fullName evidence="14">Serotonin receptor 7</fullName>
        </alternativeName>
    </protein>
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        <name evidence="19" type="primary">htr7</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A672ZKE5"/>
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        <property type="protein sequence ID" value="ENSSORP00005017641.1"/>
        <property type="gene ID" value="ENSSORG00005008726.1"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GOC"/>
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        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15329">
        <property type="entry name" value="7tmA_5-HT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000071">
        <property type="entry name" value="5-hydroxytryptamine (serotonin) receptor 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001069">
        <property type="entry name" value="5HT_7_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF199">
        <property type="entry name" value="IP13425P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00652">
        <property type="entry name" value="5HT7RECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="108"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="481" mass="53000" checksum="2ACEB80EC42448A9" modified="2020-06-17" version="1">AADNGTFGSGNMRSSFMIEDRAGGGDPGGSTNMMISEALAPRLLKIAQDAAEAAVAAAAATSPSTSSQPLVMETNGTRCGEQILSYGRVEKVLIGGVLTMLTLSTICGNLLVVISVCFVKKLRQPSNYLIVSLAVADLSVALAVMPFVSITDLIGGQWIFGQFFCNVFIAMDVMCCTASIMTLCVISIDRYLGITKPLTYPVRQNGCCMAKMILSVWLLSASITLPPLFGWAQNVNDGRVCLISQDFGYTVYSTAVAFYIPMSVMLFMYYRIYRAAKLSAAKHTITGFPREKEQVAGVLQREVEEECSTRVSRLLKSGEHHQRRKRKNQSIFKREQKAAATLGIVVGAFSFCWLPFFLVSTARPFVCGIECSCVPLWLERTLLWLGYANSLINPFIYAFFNRDLRTTYSNLLRCRYRNINRKLSAAGMHEALKLVEKPDNDASVALTTRLSDCLLLQICQPSHHNNILASYISANHVYCKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZL94</accession>
    <name>A0A672ZL94_9TELE</name>
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        <submittedName>
            <fullName evidence="2">EFR3 homolog Bb (S. cerevisiae)</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">efr3b</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFR3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZL94"/>
    <dbReference type="FunCoup" id="A0A672ZL94">
        <property type="interactions" value="1083"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018481.1">
        <property type="protein sequence ID" value="ENSSORP00005017940.1"/>
        <property type="gene ID" value="ENSSORG00005004433.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZL94"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein localization to plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR049152">
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    <dbReference type="InterPro" id="IPR051851">
        <property type="entry name" value="EFR3_Homologs"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN EFR3 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12444">
        <property type="entry name" value="PROTEIN EFR3 HOMOLOG CMP44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21052">
        <property type="entry name" value="EFR3_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="819" mass="91962" checksum="C21091CC33D477C4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZPN6</accession>
    <name>A0A672ZPN6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubulin-specific chaperone D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tbcd</name>
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    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBCD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZPN6"/>
    <dbReference type="FunCoup" id="A0A672ZPN6">
        <property type="interactions" value="1573"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018917.1">
        <property type="protein sequence ID" value="ENSSORP00005018373.1"/>
        <property type="gene ID" value="ENSSORG00005003570.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:beta-tubulin binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR033162">
        <property type="entry name" value="TBCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022577">
        <property type="entry name" value="TBCD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658">
        <property type="entry name" value="BETA-TUBULIN COFACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23579">
        <property type="entry name" value="ARM_TBCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25767">
        <property type="entry name" value="ARM_TBCD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TFCD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin-folding cofactor D ARM repeats" evidence="6">
        <location>
            <begin position="290"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin-folding cofactor D C-terminal" evidence="5">
        <location>
            <begin position="871"/>
            <end position="1056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1165" mass="130090" checksum="8BD71779FBE518B3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQT6</accession>
    <name>A0A672ZQT6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Neuropilin (NRP) and tolloid (TLL)-like 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">neto2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZQT6"/>
    <dbReference type="Ensembl" id="ENSSORT00005020184.1">
        <property type="protein sequence ID" value="ENSSORP00005019625.1"/>
        <property type="gene ID" value="ENSSORG00005009623.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQT6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000016">
        <property type="entry name" value="neuropilin and tolloid-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
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    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF26">
        <property type="entry name" value="NEUROPILIN AND TOLLOID-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="13"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="128"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="253"/>
            <end position="265"/>
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    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="260"/>
            <end position="278"/>
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    </feature>
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        <location>
            <begin position="272"/>
            <end position="287"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="491" mass="55805" checksum="68B6A143CE948CCB" modified="2020-06-17" version="1">TEHGGQTPNQNECGTWVRNINGGVFTSPNYPNTYPPNKECVYILEALPRQRIQLAFDKNYYIEPSFECRFDHIEIRDGPFGFSPLIDRFCGGKNPGLVTSTGRFMWIKFTSDEELEGLGFRIKYTFIAGKNIPAFEIGGWDGIIRSSQVDEEERVKPGDALDCIWTIRAPPQSKIYLRFMEYQMEHSNECKKNFVAVYDGSSAIENLKAKFCSTVANDVMLDNGVGVVRMWADEKSRLSRFRMLFTSFVDPPCSANTFFCHSNMCINNSLVCNGVQNCVYPWDENHCKEKRSKGLFHQITKTHGTVIGVSSGIVLVLLIISILVQMKQPRKKVVARRPGVFNKAGFQEVFDPPHYELFSLRDKEISSDLADLSEDLDSFHKLRRSSTMSRCVHEHHCGSQASVTTGGGSMKHSRTTLSSMELSYHNDFSKPPPMKTFNSTSSYKKSCYGYKQHSQTHDCDQQFMVTSHTQGRYARFSLSSGRAHEETVLFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZSB6</accession>
    <name>A0A672ZSB6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Tenascin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tnxba</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tenascin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005019755.1">
        <property type="protein sequence ID" value="ENSSORP00005019198.1"/>
        <property type="gene ID" value="ENSSORG00005009448.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZSB6"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000001">
        <property type="entry name" value="Tenascin C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF1">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025423696" evidence="11">
        <location>
            <begin position="19"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="862"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="951"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="13">
        <location>
            <begin position="1123"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="68"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="621"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="693"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="783"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="199"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="625"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="693"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="783"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1345" mass="144925" checksum="5C64831B6C144A76" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZSI4</accession>
    <name>A0A672ZSI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Smaug homolog 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Sterile alpha motif domain-containing protein 4A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115437812</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Synapse</location>
            <location evidence="10">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMAUG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZSI4"/>
    <dbReference type="Ensembl" id="ENSSORT00005020846.1">
        <property type="protein sequence ID" value="ENSSORP00005020275.1"/>
        <property type="gene ID" value="ENSSORG00005009892.1"/>
    </dbReference>
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        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein Smaug homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Smaug, PHAT domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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        <property type="entry name" value="PROTEIN SMAUG HOMOLOG 1"/>
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    <dbReference type="PANTHER" id="PTHR12515">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 4-RELATED"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SAM" evidence="14">
        <location>
            <begin position="278"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="133"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="378"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="530"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="550"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="576"/>
            <end position="594"/>
        </location>
    </feature>
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        <source>
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    <evidence type="ECO:0000256" key="2">
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041183"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020275.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="641" mass="69988" checksum="3FF529D00A419DCA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZV07</accession>
    <name>A0A672ZV07_9TELE</name>
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        <recommendedName>
            <fullName evidence="14 18">P2X purinoceptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">P2X purinoceptor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115429939</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Extracellular ATP-activated non-selective cation channel. Plays particularly important role in sensory neurons where its activation is critical for gustatory, nociceptive responses, visceral reflexes and sensory hypersensitization.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Receptor for ATP that acts as a ligand-gated ion channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer. Forms heterotrimer with P2RX2. Heterotrimeric P2RX2/3 has a ligand dose-response profile that is distinct from either homotrimeric P2RX2 or P2RX3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 18">Belongs to the P2X receptor family.</text>
    </comment>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0098794">
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        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004931">
        <property type="term" value="F:extracellularly ATP-gated monoatomic cation channel activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.490.10">
        <property type="entry name" value="atp-gated p2x4 ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003046">
        <property type="entry name" value="P2X3_purnocptor"/>
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    <dbReference type="InterPro" id="IPR027309">
        <property type="entry name" value="P2X_extracellular_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001429">
        <property type="entry name" value="P2X_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053792">
        <property type="entry name" value="P2X_RECEPTOR_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00863">
        <property type="entry name" value="P2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF8">
        <property type="entry name" value="P2X PURINOCEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005713">
        <property type="entry name" value="P2X_purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01310">
        <property type="entry name" value="P2X3RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01307">
        <property type="entry name" value="P2XRECEPTOR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01212">
        <property type="entry name" value="P2X_RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="331"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="285"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="17">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="115"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR005713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="408" mass="46134" checksum="1A4CD5C30B272E1C" modified="2020-06-17" version="1">IHTLTAGMWSCITDFFTYETTKSVVVKSWTIGIINRVVQLLIIIYFIWWVFINEKAYQITDTAIESSVLTKVNGFGVYNNTLMDVADYVTPTQGASVFCIITKLIKTENQVQGYCPEAERKFNCTTDSDCKSHTNGGVLTGRCVHINKSKGMCEIKGWCPAEVDTIKTTPMKEVENFTVFIKNSIRFPTFNYTKGNVLPTLTDEYVKKCIFDTVNNTYCPIFRVGDMVRYAQQNFTELANKGGVIGIKIDWMCDLDKSDDQCNPSYSFTRLDAMSQHNAVSPGYNFRFAKYFKMKNGTDYRTLVKAYAIRFDVLVNGNAGKFNMIPTLINMVAAFTSVGVVSSIVGVIHGNVLCDIILLNFLKGAEQYKAKKFEEVKQFINHMVQRVFYGLMHKLTSCSYKTDISFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXD5</accession>
    <name>A0A672ZXD5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibromodulin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Keratan sulfate proteoglycan fibromodulin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115422981</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds to type I and type II collagen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZXD5"/>
    <dbReference type="Ensembl" id="ENSSORT00005022320.1">
        <property type="protein sequence ID" value="ENSSORP00005021674.1"/>
        <property type="gene ID" value="ENSSORG00005010591.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZXD5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000073">
        <property type="entry name" value="Lumican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF4">
        <property type="entry name" value="FIBROMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LRRNT" evidence="15">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="337" mass="38483" checksum="47537466C679E75C" modified="2020-06-17" version="1">DLVLICFIAWISNLNFQWLSHLRGRWRSGGWWAEDMDCPLECDCPSAYPTAMYCHNRNLQHVPYVPSHIKYVYLQRNQITGIQDGVFDNATSLIWVVLFHNQLSSDKIGKNVFSKLKNLDRLYLNHNELTRVPPNLPKSITDLRLGHNKISKISGSFEGMTNLTNLQLHANFIEDVGGAFKGLKSLTMLDMRKNKLKKVPENLPARLQQLYLEFNNIEAVPAGFLSMCPKLQFVRLAHNKLTDKGLPANVFNISTLVELDLSHNKLERIPTVSRNLENLYLQANTIKEFSLGSFCSTVDMKTFSMLKMLRLDANQINAKDIPAEAAYCLRRVAFIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXK9</accession>
    <name>A0A672ZXK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Calcium/calmodulin-dependent protein kinase type IV</fullName>
            <ecNumber evidence="4">2.7.11.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">CaM kinase-GR</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">si:ch73-60h1.1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer. Interacts with protein phosphatase 2A (PPP2CA/PPP2CB); the interaction is mutually exclusive with binding to Ca(2+)/calmodulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZXK9"/>
    <dbReference type="Ensembl" id="ENSSORT00005021600.1">
        <property type="protein sequence ID" value="ENSSORP00005020973.1"/>
        <property type="gene ID" value="ENSSORG00005010240.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZXK9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000255">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000279">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="12 24 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="17" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 24 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 25" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="28"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00062378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00071328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="325" mass="37291" checksum="CB03B52641A89413" modified="2020-06-17" version="1">MPTSRPGSEPVEFWVDGSRRDGTVEEFYTLSSELGRGATSIVYRCEEKQTQKPYAIKVLKKTIDKKIVRTEIGVLLRLSHPNIIQLKEIFETDTDIALVLELVTGGELFDRIVERGYYSERDAAHVIKQILEAVAYLHENGVVHRDLKPENLLYADLSLDAPLKIADFGLSKIIDDQVTMKTVCGTPGYCAPEILRGNAYGPEVDMWSVGVILYILLCGFEPFFDPRGDQYMYSRILNCDYEFVSPWWDEVSLNAKDLVSKLIVLDPHKRLSVREALQHPWVLGKAARFSHMDTTQRKLQEFNARRKLKVRLPHMDLVQYDRSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY08</accession>
    <name>A0A672ZY08_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Glutathione reductase</fullName>
            <ecNumber evidence="21">1.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">gsr</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16 21">Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH:GSSG ratio in the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 21">
            <text>2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="18">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.7" evidence="21"/>
    <dbReference type="RefSeq" id="XP_030002031.1">
        <property type="nucleotide sequence ID" value="XM_030146171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZY08"/>
    <dbReference type="Ensembl" id="ENSSORT00005022526.1">
        <property type="protein sequence ID" value="ENSSORP00005021874.1"/>
        <property type="gene ID" value="ENSSORG00005010214.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427572"/>
    <dbReference type="CTD" id="2936"/>
    <dbReference type="InParanoid" id="A0A672ZY08"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000003">
        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000484">
        <property type="entry name" value="Glutathione reductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000671">
        <property type="entry name" value="Thioredoxin reductase 2, tandem duplicate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006322">
        <property type="entry name" value="Glutathione_Rdtase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01421">
        <property type="entry name" value="gluta_reduc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004776">
        <property type="entry name" value="PRK06116.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF2">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 21" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 18 20" id="KW-0274">FAD</keyword>
    <keyword evidence="6 20" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-0520">NAD</keyword>
    <keyword evidence="8 21" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14 20" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="23">
        <location>
            <begin position="43"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="22">
        <location>
            <begin position="391"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="491"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="215"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="19">
        <location>
            <begin position="79"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU365016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="502" mass="54796" checksum="73CCCE5FCC0B7C10" modified="2020-06-17" version="1">MRFVRICRLLSARSASLALPRHEKTIRRSMASDPSTGEPTRFDFLVIGGGSGGLAGARRAAELGAKTAVIENHKLGGTCVNVGCVPKKVMWNAAVHAEYLHDHSDYGFDVENVRFSWETLKAKRDAYVSHLNRIYRNNLDKAKIQTIHGHATFTNDPEPTVEVNGRKYTAPHILIATGGQPSVLSDAEVPGAHLGITSDGFFELERLPKRSVIVGAGYIAVEMAGILSTLGSKTSLVIRQTGVLRNFDAFISTNCTKELQNTGVDLWKNSQVTSVSKKDSGLEVTIVTKDPEKKNDEEKISTIQEVDCLLWAIGRQPNTSGLNLTELGVDTDERGHIVVDEFQNTSRSGIYAVGDVCGKALLTPVAIAAGRKLAHRLFENKKDSKLDYSSIPTVVFSHPPIGTVGLTEEEAVKLKGKENVKIYKTSFTPMYYALTTRKSPCIMKLVCVGKEETVMGLHMQGLGCDEMLQGFAVAIKMGATKADFDRTVAIHPTSSEEFVTMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A115</accession>
    <name>A0A673A115_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD repeat domain 73</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">wdr73</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030013330.1">
        <property type="nucleotide sequence ID" value="XM_030157470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A115"/>
    <dbReference type="FunCoup" id="A0A673A115">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023838.1">
        <property type="protein sequence ID" value="ENSSORP00005023165.1"/>
        <property type="gene ID" value="ENSSORG00005011227.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435203"/>
    <dbReference type="CTD" id="84942"/>
    <dbReference type="InParanoid" id="A0A673A115"/>
    <dbReference type="OrthoDB" id="9822052at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042795">
        <property type="entry name" value="Wdr73"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46947">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46947:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="343"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="41023" checksum="FF3A255F0CB4295C" modified="2020-06-17" version="1">MEEDLDNILDDWFIESIKIYKDLHVYQLENPTQVLEWTSGRNVCVAGYTSSSKNEILELHLPLKLLADKNKGLCAERDFKVVHGGFADGPVRRLRHIPGTRCAVTNDGHSSSLQVWDLGGDDSDVIRRTGSVEGGRTGSVRGSRIAARPSSQPQVLHGAQSDDVRLTEITSGRTVYKLEVDSSDALSSLQFVTDSIVVAGCCKGSILVADIRSPAAPQVCCPPASSAESVLWWTDASASSSRLIRLSSCGQAVVSELRMLGGGAVCQAQLDIQMRGSDSEDVVVSWAPALDDCIAVSGFSGAVQIYNTSLWKSDLQETTPLFQHRGHMVSSQQPDGVLTTAHAWHPERPRTLLSAASDGSVHVWDWVNQSAAESGSADPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1K7</accession>
    <name>A0A673A1K7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sorcin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sri</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A1K7"/>
    <dbReference type="FunCoup" id="A0A673A1K7">
        <property type="interactions" value="302"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023834.1">
        <property type="protein sequence ID" value="ENSSORP00005023161.1"/>
        <property type="gene ID" value="ENSSORG00005011223.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A1K7"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16181">
        <property type="entry name" value="EFh_PEF_Group_II_sorcin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735">
        <property type="entry name" value="CALPAIN, SMALL SUBUNIT 1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735:SF7">
        <property type="entry name" value="SORCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="184" mass="20507" checksum="992AC827810C3C7C" modified="2020-06-17" version="1">MAYHGYGAAPGGFPPAQQDPMYGYFAAVAGQDGQISADELQRCLTQSGMSGSYKPFSLETCRLMISMLDRDYSSTMGFNEFKELGQVLTGWRNVFASYDRDHSGTVEGHELQQAIAGLGYNLSPQAMNIIMKRFSTNGRIPFDEFVSCCVKLRALTDQFRRRDQSQTGHASFGYDDFIQVTMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2S8</accession>
    <name>A0A673A2S8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Plexin domain containing 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">plxdc1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029996870.1">
        <property type="nucleotide sequence ID" value="XM_030141010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A2S8"/>
    <dbReference type="Ensembl" id="ENSSORT00005024220.1">
        <property type="protein sequence ID" value="ENSSORP00005023539.1"/>
        <property type="gene ID" value="ENSSORG00005011398.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423965"/>
    <dbReference type="InParanoid" id="A0A673A2S8"/>
    <dbReference type="OrthoDB" id="6285106at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031152">
        <property type="entry name" value="PLXDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055:SF10">
        <property type="entry name" value="PLEXIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055">
        <property type="entry name" value="TUMOR ENDOTHELIAL MARKER 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025612934" description="Plexin domain containing 1" evidence="9">
        <location>
            <begin position="23"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="54457" checksum="5B1A6DA5372B6F7C" modified="2020-06-17" version="1">MFLRLMMLLICLSQTELASVWAQKTDNLYTVISEQHVESTRGDSQAHTAQSSSSGHSRINRAVLVGGITVDTWPNNMTHVVEDSGKYYTWRSYGPEDQRTKELWVDMSNLRHGQVRVHGILSNTHKQAARVALSFDFPFYGHFLRQITIATGGFIFTGDITHRMLTATQYIAPLMANFDPSFSKESTVQYLDNGEVFVIQWERVRLQGKESEGAFTFQAALYKSGTITFSYKDIPLSLDVISSAEHPVKAGLSDAFTVSSPSTQSPDNTQRQTIYEYHRVEINTTKITNYSAIEFTALPTCLQHDSCDLCLSSNQTSGCSWCNVLQRCSDGMDRHRQEWFDYACSEESKNATCEDYSRDDTSSITPETEGVTSLIPRQKGCESVDETKHHVFKTGSDVKTDPSIKNEGLPNTAVIAGIAAALVLLLALILVALYINCHPHAASPLYLIQRRKSYWPSLKFQKQQTGYTEVEGEGHHEKDSIVEAGPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A512</accession>
    <name>A0A673A512_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">wnt9b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997349.1">
        <property type="nucleotide sequence ID" value="XM_030141489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A512"/>
    <dbReference type="FunCoup" id="A0A673A512">
        <property type="interactions" value="665"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025287.1">
        <property type="protein sequence ID" value="ENSSORP00005024570.1"/>
        <property type="gene ID" value="ENSSORG00005011819.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424307"/>
    <dbReference type="CTD" id="7484"/>
    <dbReference type="InParanoid" id="A0A673A512"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19354">
        <property type="entry name" value="Wnt_Wnt9b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000002">
        <property type="entry name" value="Protein Wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF84">
        <property type="entry name" value="PROTEIN WNT-9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025340365" description="Protein Wnt" evidence="12">
        <location>
            <begin position="27"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="355" mass="39746" checksum="04164A7A17200B15" modified="2020-06-17" version="1">MRSRLPRTACLLRLIALCILLSHTAAYFGLTGREPLVFLPGPFSNEPPNGKAHLKQCEQMTLTRRQKRLCRREPGLAETLRESVRLSLLECRYQFRNERWNCSLDGRGSLLKRAFKETAFLLAVSSAALTHALAKACSSGRMERCTCDDSPGIQHREAWQWGVCGDNLKYSTKFLKKFLGQKRVSKDLRAQVDAHNINVGIRAVKSGLKTTCKCHGVSGSCAVRTCWKQLSPFHDTGRLLKYRYDTAVRVLSVTNAATGESELAGPRRHGQSLRTTDLVYLEDSPSFCRPSRYSAGTGGRSCAKDTSCQNLCCGRGYNTAMRLTSLSCHCQVRWCCHVECQTCVREEEVYTCKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A561</accession>
    <name>A0A673A561_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dihydropteridine reductase</fullName>
            <ecNumber evidence="7">1.5.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HDHPR</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Quinoid dihydropteridine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">qdpra</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of quinonoid dihydrobiopterin into tetrahydrobiopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6,7,8-tetrahydropteridine + NAD(+) = 6,7-dihydropteridine + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17869"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28889"/>
            <dbReference type="ChEBI" id="CHEBI:30156"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.34"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:17871"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6,7,8-tetrahydropteridine + NADP(+) = 6,7-dihydropteridine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28889"/>
            <dbReference type="ChEBI" id="CHEBI:30156"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.34"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:17867"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.34" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673A561"/>
    <dbReference type="FunCoup" id="A0A673A561">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024466.1">
        <property type="protein sequence ID" value="ENSSORP00005023767.1"/>
        <property type="gene ID" value="ENSSORG00005011486.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A561"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004155">
        <property type="term" value="F:6,7-dihydropteridine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070404">
        <property type="term" value="F:NADH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05334">
        <property type="entry name" value="DHPR_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000157">
        <property type="entry name" value="Quinoid dihydropteridine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15104">
        <property type="entry name" value="DIHYDROPTERIDINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15104:SF1">
        <property type="entry name" value="QDPRA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="293" mass="30791" checksum="22F640FE4BE7B6BF" modified="2020-06-17" version="1">MKLCLKRTPFVRVSLKASLVPPTCCSSGGTRPPLRPRISSAAPLWSVTANAAKSNTNMAANRVLVYGGKGALGSKCVQHFKSKGWWVASIDLAANEEANENVIVKLTESFTEQAGQVSADVAQLLGEQKVDAILCVAGGWAGGNCNSKDLYKNTDLMWKQSVWTSTISSHLAARHLKADGLLTLAGAKAALSGTGGMVGYGMAKAAVHQLCQSLAAKNSGMPSGAAAVTILPVTLDTPMNRKFMPDADFSSWTPLEYIAELFFNWATGVDRPASGSLMQLLTSGGETQAVATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5F7</accession>
    <name>A0A673A5F7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Golgi reassembly stacking protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115437943</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GORASP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A5F7"/>
    <dbReference type="Ensembl" id="ENSSORT00005024606.1">
        <property type="protein sequence ID" value="ENSSORP00005023903.1"/>
        <property type="gene ID" value="ENSSORG00005011542.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A5F7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000026">
        <property type="entry name" value="Golgi reassembly stacking protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000056">
        <property type="entry name" value="Golgi reassembly-stacking protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF1">
        <property type="entry name" value="GOLGI REASSEMBLY-STACKING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="11">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ GRASP-type" evidence="11">
        <location>
            <begin position="111"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="237"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR607583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="277" mass="29710" checksum="531B71EC43CBF211" modified="2020-06-17" version="1">MGGSQSVEIPGGGSEGYHVLRVQENSPGHRAGLEPFFDFIVSINNTRLNKDNDTLKDLLKASVEKPVKMLVYSSKTLELRESTVTPSNLWGGQGLLGVSIRFCSFEGANENVWHVLEVEPNSPAALAGLRPHTDYIIGADTVMNESEDLFSLIESHEGKGLKLYVYNTDTDNCREVVITPNSSWGGEGSLGCGIGYGYLHRIPTRPFEEGKKFSFPGNSPSEPVSPLKDGFTEVQLSAVTPPPTAPTVPSGLEDSLSGLSISTTAPTMPSELQTGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AC45</accession>
    <name>A0A673AC45_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Interferon a3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115410379</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AC45"/>
    <dbReference type="FunCoup" id="A0A673AC45">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027609.1">
        <property type="protein sequence ID" value="ENSSORP00005026826.1"/>
        <property type="gene ID" value="ENSSORG00005012850.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AC45"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043330">
        <property type="term" value="P:response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF73">
        <property type="entry name" value="INTERFERON BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025574683" evidence="8">
        <location>
            <begin position="23"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="249" mass="28412" checksum="3BA442169E25AA2F" modified="2020-06-17" version="1">MPNKIFFVCLFVSLFCAGASLSCRWMEQNFRQYNENSLTLLENMVTNSTNSTVDAAEEDAVAFPDELYKHASKASAEDRLAFTVQILEEVAALFEEDRSPSNETVWEETTVENFINLTTQQADGLRSCIASHSHKKKNKKLSLYFKRLSHHAKQMIHSADTWELIRKEAHTHLHRADILVTSVLTVNVSPLIDQGVEQALYCLQNRANLSSQSTTLNITSIHEFFCFSFTFVHLLFDKHFCGLFCHLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACS0</accession>
    <name>A0A673ACS0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein phosphatase methylesterase 1</fullName>
            <shortName evidence="7">PME-1</shortName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ppme1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48548"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="3.1.1.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Binds PPP2CA and PPP2CB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673ACS0"/>
    <dbReference type="FunCoup" id="A0A673ACS0">
        <property type="interactions" value="1757"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027798.1">
        <property type="protein sequence ID" value="ENSSORP00005027014.1"/>
        <property type="gene ID" value="ENSSORG00005012905.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ACS0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022950">
        <property type="entry name" value="PPase_methylesterase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="10">
        <location>
            <begin position="70"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="226"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR022950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR022950-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="373" mass="41203" checksum="59B3A486DE1776A5" modified="2020-06-17" version="1">MEKQLHLNLLASRPPMAGGFQSGSKMKMGPGRKRDFTPLPWSHSFMTVCISLTNVNIFRIYCSGSHGPVLLLLHGGGHSALSWAVFTAVICSRINCRVVAMDLRAHGDTKVKNPEDLSADTMAKDIGKVVESLYGENPPPIVIIGHSMGGAIAVHTATANHIPSLLGLCVIDVVEGTAMDALNSMQNFLRSRPKTFKSLENAIEWSVKSGQIRNIESARVSMGGQVKKCEETTSGPGVSNSIGEGIIEEEEDEEAEEESNKKRMKEDDQEMKKESIFTWRVELSKTEKYWEGWFRGLSALFLTCPVPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPEKVADALATFMVRHKFTEFKDGYLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADN0</accession>
    <name>A0A673ADN0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Angiopoietin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">angpt1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Homooligomer. Interacts with TEK/TIE2. Interacts with SVEP1/polydom. Interacts with THBD; this interaction significantly inhibits the generation of activated PC and TAFIa/CPB2 by the thrombin/thrombomodulin complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ADN0"/>
    <dbReference type="Ensembl" id="ENSSORT00005027366.1">
        <property type="protein sequence ID" value="ENSSORP00005026587.1"/>
        <property type="gene ID" value="ENSSORG00005012730.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ADN0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000005">
        <property type="entry name" value="angiopoietin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.530.10:FF:000001">
        <property type="entry name" value="angiopoietin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057439">
        <property type="entry name" value="ANG-1/2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25443">
        <property type="entry name" value="ANG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025678942" description="Angiopoietin-1" evidence="14">
        <location>
            <begin position="27"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="15">
        <location>
            <begin position="313"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="205"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="534" mass="60312" checksum="1581AD33B369586F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AH99</accession>
    <name>A0A673AH99_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spindlin-Z-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115437646</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIN/STSY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016797.1">
        <property type="nucleotide sequence ID" value="XM_030160937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AH99"/>
    <dbReference type="Ensembl" id="ENSSORT00005029900.1">
        <property type="protein sequence ID" value="ENSSORP00005029080.1"/>
        <property type="gene ID" value="ENSSORG00005013879.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437646"/>
    <dbReference type="InParanoid" id="A0A673AH99"/>
    <dbReference type="OrthoDB" id="9944558at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.70:FF:000001">
        <property type="entry name" value="Spindlin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.70">
        <property type="entry name" value="Spindlin/Ssty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003671">
        <property type="entry name" value="SPIN/Ssty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042567">
        <property type="entry name" value="SPIN/Ssty_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10405">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02513">
        <property type="entry name" value="Spin-Ssty"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="239" mass="27615" checksum="E0FD8D2F54A349B0" modified="2020-06-17" version="1">MSKKRGRKRSSGELSESSASTLSPDPDNLLGRRIQHTWREKGNVTKWKGTVLERLTVNSSLYMVKYDGFDCVYGIELFKDSRVSNLQVLAEKVVNNKIKVPPGAEELVGRAVEHLFEKEDGEKNEWRGMVLSRAPIMTHWYYITYEKDPVLYMYQLWDDYKDGDLRVLPESENKHLLPADRKPGEEPESLVGKQVEYVTDNGLKRTGLVIYQVPAKPTVYYIKYDDDVHIHVYDLVKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIT8</accession>
    <name>A0A673AIT8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Centrosomin N-terminal motif 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AIT8"/>
    <dbReference type="Ensembl" id="ENSSORT00005030104.1">
        <property type="protein sequence ID" value="ENSSORP00005029271.1"/>
        <property type="gene ID" value="ENSSORG00005013975.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AIT8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097431">
        <property type="term" value="C:mitotic spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000242">
        <property type="term" value="C:pericentriolar material"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
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            <begin position="71"/>
            <end position="110"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF07989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="14693" checksum="B414B5FB023EB8D2" modified="2020-06-17" version="1">MPSPLLPPFSPRLLFHPSPLAVWDSRDVSSRKISSCPSPLQTRTHHGADMAASLTLCRWSLTVMSNGYRTLSQHLNDLKKENFSLKLRIYFLEERIQQKYEESSEDVYRTVSNAALISYPDMNGSMMDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AM31</accession>
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        <submittedName>
            <fullName evidence="4">von Willebrand factor A domain containing 5B2</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">vwa5b2</name>
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    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="VWF_A"/>
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    </dbReference>
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        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5B2"/>
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    <feature type="domain" description="VWFA" evidence="3">
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            <begin position="330"/>
            <end position="392"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="813"/>
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            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="830"/>
            <end position="840"/>
        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF13757"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13768"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="953" mass="105027" checksum="20DA07730F1582FD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AML5</accession>
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        <recommendedName>
            <fullName evidence="11">Histone H2A</fullName>
        </recommendedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2A family.</text>
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        <property type="nucleotide sequence ID" value="XM_030122633.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005029648.1"/>
        <property type="gene ID" value="ENSSORG00005014151.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030490.1">
        <property type="protein sequence ID" value="ENSSORP00005029657.1"/>
        <property type="gene ID" value="ENSSORG00005014156.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005029747.1"/>
        <property type="gene ID" value="ENSSORG00005014215.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058560.1">
        <property type="protein sequence ID" value="ENSSORP00005057255.1"/>
        <property type="gene ID" value="ENSSORG00005025397.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410814"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="14">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="128" mass="13715" checksum="7ABD41610800783B" modified="2020-06-17" version="1">MSGRGKTGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTEKPGKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQM5</accession>
    <name>A0A673AQM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450 26A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115439264</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>all-trans-retinoate + reduced [NADPH--hemoprotein reductase] + O2 = all-trans-(4S)-hydroxyretinoate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51492"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35291"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:134185"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:51493"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030018943.1">
        <property type="nucleotide sequence ID" value="XM_030163083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AQM5"/>
    <dbReference type="FunCoup" id="A0A673AQM5">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032654.1">
        <property type="protein sequence ID" value="ENSSORP00005031771.1"/>
        <property type="gene ID" value="ENSSORG00005015114.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439264"/>
    <dbReference type="CTD" id="1592"/>
    <dbReference type="InParanoid" id="A0A673AQM5"/>
    <dbReference type="OrthoDB" id="1372046at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008401">
        <property type="term" value="F:retinoic acid 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034653">
        <property type="term" value="P:retinoic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000041">
        <property type="entry name" value="Cytochrome P450 26A1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF101">
        <property type="entry name" value="CYTOCHROME P450 26A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 16 17" id="KW-0349">Heme</keyword>
    <keyword evidence="10 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 17" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="487" mass="55378" checksum="CCADBF09B44D5281" modified="2020-06-17" version="1">MALNTLLASVLCTLVLPLLLILVALKLWEVYTVRGRDPGCPRPLPPGSMGLPFIGETLQLILQRRKFLRMKRQKYGYIYRTHLFGNPTVRVTGAENVRHLLLGEHRLVSVQWPASVRAILGSDTLSNVHGAQHKTKKKAILRAFSRDALELYIPVIQEEVQVAVKDWLGRDSCVLVYLEMKRLMFRIAMRILLGFEPEQIKMDEQQLVEAFEEMIKNLFSLPIDVPFSGLYRGLKARNFIHSKIEENIKKKVRDSDKDPKHRDALQQLIDSSRKNREPLSMQAIKESATELLFGGHETTASTATSLIMFLGLNPDAVDLLRQELMDKEEQGMDLQTLNIESLEQLKYTSCVIKETLRINPPVPGGFRVALKTFELNGFQIPKGWNVIYSICDTHDVAEVFPNKDEFQPERFMTKADSSSRFQYIPFGGGSRMCVGKEFAKVLLKIFLVEVVTKCHWTLLNGPPTMKTGPTVYPVDNLPTKFMSYGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARS1</accession>
    <name>A0A673ARS1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ATPase H+ transporting accessory protein 1 like b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vacuolar ATPase subunit S1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ARS1"/>
    <dbReference type="Ensembl" id="ENSSORT00005032001.1">
        <property type="protein sequence ID" value="ENSSORP00005031133.1"/>
        <property type="gene ID" value="ENSSORG00005014848.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARS1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033176">
        <property type="term" value="C:proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030641">
        <property type="term" value="P:regulation of cellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008388">
        <property type="entry name" value="Ac45_acc_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046755">
        <property type="entry name" value="VAS1_LD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471:SF3">
        <property type="entry name" value="ATPASE, H+ TRANSPORTING, LYSOSOMAL ACCESSORY PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05827">
        <property type="entry name" value="VAS1_LD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1 luminal" evidence="7">
        <location>
            <begin position="18"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="8">
        <location>
            <begin position="94"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="153" mass="17262" checksum="FA0DBBAAF9D364CD" modified="2020-06-17" version="1">VISLVLHFHGLQRLLQDIEYKAFQATFNASEVYAPATSSYHCQHVSSLHKYDTLLVPSSHTDTSANWHITFTDFQIQAFNVQSDKFASASDCATFLTPAILMGLVTSLILLLVLAYALHMVVHLKHIDRYEEHKATVYFPRSPEAELPDKNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARS6</accession>
    <name>A0A673ARS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ARS6"/>
    <dbReference type="Ensembl" id="ENSSORT00005031948.1">
        <property type="protein sequence ID" value="ENSSORP00005031082.1"/>
        <property type="gene ID" value="ENSSORG00005014824.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARS6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008046">
        <property type="term" value="F:axon guidance receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000022">
        <property type="entry name" value="Cardiac titin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050958">
        <property type="entry name" value="Cell_Adh-Cytoskel_Orgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080">
        <property type="entry name" value="CONTACTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080:SF8">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="100"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="226" mass="24672" checksum="C9E3D6AD5B4234FC" modified="2020-06-17" version="1">KKFIKYLFPESVNVKSGENVALEVRVSGSPELKTKWYKDNKELSVGAKYQMSFTKKVAILKIRSADKTDAGEYKLDVSNNVGTASCRTKLSVSDTHLVVGRPGEMECKVAGSAPLTTSWFHNGQEIKSGPNCEISCSDNNCRLSFPSIGLYDSGKYTCKAVNAAGSSDTSASMSVTEPPSFAETPEKMETLPGKNVSFTAKVRGTVLFYNSSWNYLPQNQFISSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUR2</accession>
    <name>A0A673AUR2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transcription factor 7-like 1-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tcf7l1a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AUR2"/>
    <dbReference type="Ensembl" id="ENSSORT00005032995.1">
        <property type="protein sequence ID" value="ENSSORP00005032108.1"/>
        <property type="gene ID" value="ENSSORG00005015259.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUR2"/>
    <dbReference type="OrthoDB" id="2307332at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071664">
        <property type="term" value="C:catenin-TCF7L2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010456">
        <property type="term" value="P:cell proliferation in dorsal spinal cord"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060847">
        <property type="term" value="P:endothelial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021903">
        <property type="term" value="P:rostrocaudal neural tube patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21996">
        <property type="entry name" value="HMG-box_TCF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000001">
        <property type="entry name" value="transcription factor 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000002">
        <property type="entry name" value="transcription factor 7-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013558">
        <property type="entry name" value="CTNNB1-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF32">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="HMG box" evidence="12">
        <location>
            <begin position="302"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="10">
        <location>
            <begin position="302"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="259"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="368"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="500"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="459"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="517"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="548"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="601" mass="65460" checksum="D22375773216C012" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AV25</accession>
    <name>A0A673AV25_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">prkg1l</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673AV25"/>
    <dbReference type="Ensembl" id="ENSSORT00005034379.1">
        <property type="protein sequence ID" value="ENSSORP00005033471.1"/>
        <property type="gene ID" value="ENSSORG00005015848.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AV25"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    <sequence length="635" mass="71782" checksum="7D6900768B43FBDD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
    <sequence length="351" mass="40286" checksum="1CD502E4060F501B" modified="2020-06-17" version="1">HTSRPWVAHSFLDPDTSTCSSTFCFTEDRMNNTTCHSIDFDFPGRFLPPVYILVFIIGLVANGWGLKSLLQNWKKLGNVNVFVFNLGLTDVLYLLTLPFIVVYYFMDSTWVFGDTFCKITRFCFNLNLYGSIGFLTCISVYRYLAIVHPMKVMGRLSVTHSVVISVLVWLLVSVQSLPDMFYTKTSINMTKCYDTTSDVYVEDYLKYYLGWTLTGFCIPLLITLGCYGHVIVTLCRKENIDKVLKQKSLMLLVILVVLFSVCYIPYHILKNLNLWSRVLQQRRTCLGWYDTVYIARQISRGLVSLNSALNPLVYLHGSEVAPAQLRKLFQQARQAVMRQASTPTQMSLMQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AX18</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <scope>IDENTIFICATION</scope>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="SDR_fam"/>
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        <property type="entry name" value="adh_short"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="54" mass="5832" checksum="226EE93F14448EA4" modified="2020-06-17" version="1">MWSRSPPVLPNQRGRVAIVTGGTRGMGLETAKQLVRLGMHVIIGRTPTNHKAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AXC3</accession>
    <name>A0A673AXC3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetratricopeptide repeat domain 39A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ttc39a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AXC3"/>
    <dbReference type="Ensembl" id="ENSSORT00005035320.1">
        <property type="protein sequence ID" value="ENSSORP00005034401.1"/>
        <property type="gene ID" value="ENSSORG00005013031.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AXC3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF3">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="584" mass="66684" checksum="1A8F1439D342D6BE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYF8</accession>
    <name>A0A673AYF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Translation initiation factor eIF2 assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cdc123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">ATP-dependent protein-folding chaperone for the eIF2 complex. Binds to the gamma subunit of the eIF2 complex which allows the subunit to assemble with the alpha and beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CDC123 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AYF8"/>
    <dbReference type="FunCoup" id="A0A673AYF8">
        <property type="interactions" value="1109"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035770.1">
        <property type="protein sequence ID" value="ENSSORP00005034846.1"/>
        <property type="gene ID" value="ENSSORG00005016422.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AYF8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007807">
        <property type="entry name" value="Cdc123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR007807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="344" mass="39547" checksum="7133DC7F5F5924C3" modified="2020-06-17" version="1">MKKEQVVNCQFSVWYPIFKKHTIKSLILPLPQNVIDYLLDDGTLVVSGSDHNTQQTHTNNSDSDAEEDVQWSDDETTTTVTAPEFPEFTSKVLEAINALGGSIFPKLNWSAPRDANWIALNSSLQCCSLSDIFLLFKSSDFITHDLTQIDLMPSMYVITSLHCVICPQLVLRKWSELIPGGEFRCFVKENKLIAISQRDYTQYYQHILKQEEPVCQAIQDFFSQHIQYNFLDEDFVFDVYRDSQGRVWLIDLNPFGEVTDSLLFSWEELTSGGELNQHQEGPAFRYTTSEVTVQPSPCLSYRIPRDFVDLSTGEDAYKLIDFLKLTKSQQEECEEEEEEENAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3D7</accession>
    <name>A0A673B3D7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">U1 small nuclear ribonucleoprotein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">snrpa</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM U1 A/B'' family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B3D7"/>
    <dbReference type="Ensembl" id="ENSSORT00005037712.1">
        <property type="protein sequence ID" value="ENSSORP00005036746.1"/>
        <property type="gene ID" value="ENSSORG00005017306.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3D7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12477">
        <property type="entry name" value="RRM1_U1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052285">
        <property type="entry name" value="NEXT_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034407">
        <property type="entry name" value="U1A_RRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13798">
        <property type="entry name" value="RNA BINDING MOTIF RBM PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13798:SF11">
        <property type="entry name" value="RNA-BINDING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="278" mass="30359" checksum="483651A9885FBCDD" modified="2020-06-17" version="1">MATTEVRLNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVARNLKMKGQAFVIFKEINSASNALRSMQGFPFYDKPMRIQYAKQDSDIIAKMKGTFVERDRKKEKKKPKGPDAAGAKKGVPGAAAPMVAGVPAAMPGMPPMSQAPRMMHMPGQPPYMPPPGMMPPPGMAPGQMPPGAMPPGQMMPGQMPGQMPQQVSRKSTKSHPVPHQPPRRNKRTHGSPCSSTSECQLCCRCTVLVGLYSVPNWTFVVHSGSQDSGGTSGPRSPRYRLCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3U9</accession>
    <name>A0A673B3U9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">irx5a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B3U9"/>
    <dbReference type="FunCoup" id="A0A673B3U9">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036987.1">
        <property type="protein sequence ID" value="ENSSORP00005036029.1"/>
        <property type="gene ID" value="ENSSORG00005017008.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3U9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003893">
        <property type="entry name" value="Iroquois_homeo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF17">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00548">
        <property type="entry name" value="IRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="115"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="117"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="175"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="327"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="452" mass="48661" checksum="10682D1263BB83CD" modified="2020-06-17" version="1">MAYPQGYLYQPSASLALYSCPAYSTSVISGPRTEELGRSSSGSAFAPYAGSTAFTSASPGYNSHLPYSADAAAAATFTSYVSSPYDHTTGMAGSIGYHPYAAPLGTYPYGDPAYRKNATRDATATLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWTPRNRSEDEEEDENIDLEKNDDDEPNKPTDKGDSTDTEADHKLLNPGDLGCERFKEENHGKDTDPLLSDSELKDPEERTTELLPDSAKPTTSSPSAVPRGNQAVAQQDKPSDLSLASGTVTSNVTSVIHSPPSAPKPKLWSLAEIATSSDRCKGSGDASQACPGLGQSAGMGSNASPSRSSPQCPLPNSTVLSRPLYYTSPFYPGYTNYGGTFGHLHSNHGSVTTGSTAHFNGLNQTVLNRAEALVRESQKVRGQTQVDLCKDSPYELKKGMSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B7F9</accession>
    <name>A0A673B7F9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inter-alpha-trypsin inhibitor heavy chain H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">itih3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITIH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B7F9"/>
    <dbReference type="FunCoup" id="A0A673B7F9">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037354.1">
        <property type="protein sequence ID" value="ENSSORP00005036393.1"/>
        <property type="gene ID" value="ENSSORG00005017015.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B7F9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030212">
        <property type="term" value="P:hyaluronan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000013">
        <property type="entry name" value="inter-alpha-trypsin inhibitor heavy chain H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010600">
        <property type="entry name" value="ITI_HC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF115">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06668">
        <property type="entry name" value="ITI_HC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="VIT" evidence="13">
        <location>
            <begin position="208"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="463"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1030" mass="115720" checksum="B695F1477D0100D8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB49</accession>
    <name>A0A673BB49_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibroblast growth factor</fullName>
            <shortName evidence="2">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fgf11b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BB49"/>
    <dbReference type="Ensembl" id="ENSSORT00005038834.1">
        <property type="protein sequence ID" value="ENSSORP00005037847.1"/>
        <property type="gene ID" value="ENSSORG00005017755.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BB49"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23309">
        <property type="entry name" value="beta-trefoil_FGF11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000001">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="190" mass="21426" checksum="590EFE7B087ECD63" modified="2020-06-17" version="1">MLLFSVSEPQLKGIVTRLYSQHGYYLQMQPDGTMDSTRDESSSFSQFNLIPVGLRIVAIQGAKTGLYLAMNSEGYLYTSEHFTPECKFKESVFENYYVTYSSMLYRQTQSGRSWYIGINRDGQIMKGNRVKKTKGAAHFLPKVIEVAMYKEPSLHELASEPVSPPRKTVKTSDSLSLKNGRKEAPKADAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BE84</accession>
    <name>A0A673BE84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115412030</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029980188.1">
        <property type="nucleotide sequence ID" value="XM_030124328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BE84"/>
    <dbReference type="Ensembl" id="ENSSORT00005041894.1">
        <property type="protein sequence ID" value="ENSSORP00005040841.1"/>
        <property type="gene ID" value="ENSSORG00005019068.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412030"/>
    <dbReference type="InParanoid" id="A0A673BE84"/>
    <dbReference type="OrthoDB" id="10017003at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF847">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="35340" checksum="97C76170E5DA1DEE" modified="2020-06-17" version="1">MDHEYNLSYITLTGHVEVEKYRYVYFLIMLITYILIICCNSTIVCLIFLHKNLHEPMYMFIAALLWNSILFSTTIYPKLLIDFLSEIQVITYSACLFQFFLFYSLGASEFLLLAAMSYDRYASICKPLQYTTIMTQTTVTVVLVLSWLLPACLILVLVILSADMKLCEFTLKGIFCNNSVYQLTCVNSKVRSIFGAVTFSNIVVFPILFIIFTYSKIFIISFQSGSEVRKKAAQTCLPHLLVLVSFTCLCIFDVSIARLESELPKLARLIMTLQVVSYHPLFNPIIYGLKMTEISKHLKRLFCPSRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEN4</accession>
    <name>A0A673BEN4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 24 member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115424856</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BEN4"/>
    <dbReference type="Ensembl" id="ENSSORT00005040783.1">
        <property type="protein sequence ID" value="ENSSORP00005039749.1"/>
        <property type="gene ID" value="ENSSORG00005018569.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BEN4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000037">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000005">
        <property type="entry name" value="sodium/potassium/calcium exchanger 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF42">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="535"/>
            <end position="560"/>
        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BH55</accession>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLZ1</accession>
    <name>A0A673BLZ1_9TELE</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="15">Presynaptic cell membrane</location>
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        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="component" value="Chromosome 18"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="neurexin-1 isoform X1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
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        <property type="entry name" value="NEUREXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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        <property type="entry name" value="LAM_G_DOMAIN"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
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    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="5" id="KW-0732">Signal</keyword>
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        </location>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="546"/>
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        </location>
    </feature>
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        <location>
            <begin position="586"/>
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        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="693" mass="75544" checksum="BA53DF968A5B9578" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMB9</accession>
    <name>A0A673BMB9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Metalloreductase STEAP4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC115435780</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 Cu(+) + NADP(+) + H(+) = 2 Cu(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71771"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:71773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 Fe(2+) + NADP(+) + H(+) = 2 Fe(3+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:71769"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the STEAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BMB9"/>
    <dbReference type="FunCoup" id="A0A673BMB9">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042807.1">
        <property type="protein sequence ID" value="ENSSORP00005041737.1"/>
        <property type="gene ID" value="ENSSORG00005019369.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BMB9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000051">
        <property type="entry name" value="STEAP2 metalloreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF5">
        <property type="entry name" value="METALLOREDUCTASE STEAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="357"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="22">
        <location>
            <begin position="33"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="21">
        <location>
            <begin position="258"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="484" mass="54002" checksum="9CB72936544A7AB9" modified="2020-06-17" version="1">MTVVSGMTEEMKPESVALHHLGAAAALDPELLCIFGTGDLGRSLGQRLLQSGYKVVYGSRRPHSCGPVPQGAQVMSHAAAARSASLIFICVHREHYDFIEMMAPHLEGKVLVDLSNNLKKDMYPEANAVYLQRLVPGAEVVKGLNTLSAWALQNGLLAGKQVFLCGNSAKAKHAVAEMATKLGLTVLDRGSLSAARELEDFPLQLFPQWRLPLCVALGLTAFFYFYLLIRDVLYAYFEDGKDISFRIMVSLANKVFPIVSLIMLSLCYLPGVIAAFIQLYRGTKYSRFPDWLDRWMLCRKQIGLIALGFALLHAIYTFIIPIRYAVRHKLISRVVDENNKTTPFNFDNTEAWATDSFYVLGILGFFLYVLLGLTSLPSVGGSLSWREFNFVQSKLGHLTLTICTAHGYIYGWNKFLRPSTYKWYTPPGYMLCLVVPSIVLVLKMLLLLPCHSRANELLVCSAREPSGTSIARATTLEAWLQREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNS8</accession>
    <name>A0A673BNS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleoporin NUP42</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nucleoporin-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nup42</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BNS8"/>
    <dbReference type="FunCoup" id="A0A673BNS8">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042935.1">
        <property type="protein sequence ID" value="ENSSORP00005041863.1"/>
        <property type="gene ID" value="ENSSORG00005019436.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BNS8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051767">
        <property type="entry name" value="Nucleoporin_NUP42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="3" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="67"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="320"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="341"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="387" mass="40219" checksum="9FA4A258BD9215D2" modified="2020-06-17" version="1">MTVCNFFLQGRCRYGDKCWNEHPRGGGYSNNYSRSSQQQQQPRGGGGFGNRVWVNPANQKGRTYIQPSSFAHDDDWGRGGGADWGRGGGGGGGGADWGGGGGGGRRDNVKSSEFSFSQNRFSALNSPSTFDKGGRGGGTVIQADIEIWESSGQWGFSCYSCTKTPVSGFTDLSPEELRLEYYTTRASGDLQNYINGVNQLLNQWRNRIQELKIMNATTRAALLAELSNPTPQGSSSDFGGSGSHTFGFGSKGEDEISLASAQPPSGFGSSSSNSASTFSFAASNASKPPTASAFGSASEFSFSSSTGGGFGTGFGTEAAATSGGSGVSGQTSGGFGPTPVASASGTSDSLFSTKSSLTPEELDQFKAKRFTLGQIPLKPPPADMLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQ81</accession>
    <name>A0A673BQ81_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BQ81"/>
    <dbReference type="Ensembl" id="ENSSORT00005043441.1">
        <property type="protein sequence ID" value="ENSSORP00005042363.1"/>
        <property type="gene ID" value="ENSSORG00005019641.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQ81"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000045">
        <property type="entry name" value="Potassium voltage-gated channel subfamily S member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF39">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY S MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="15">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="162"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="49095" checksum="ADD7792795371772" modified="2020-06-17" version="1">MGYGQILHRRGTEEDQIHLTWEGEAAIWSWCDDYSPAEKEYYFDRNREFSLCVLNFYQTGRIHMMEELCVFSFSQEIEYWGIQELHLSPCCSNWYHERKEYIEDRDWDIRSEDGHQPSFDSSFEELSALDKDLAKFKGAWCAELRSYVWLRLEDPGHSRASKIIAVASLSVVLTSIVAMCVHSMPEFQRLDENDKPIEDPVLAILEEICIACFSAEFIIRLIVAPSRRKFLGNPLNIIDVASILPFYATLALETADEESAEENEDLENVGKVVQVLRLMRVLRILKLARHSIGLRALGATVRHSYHEVGLLLLFLSVGISIFSALIYFAEKEEEETDLGTIPSGWWWATITMTTVGYGDTCPVTLAGKIVATLCIVCGLLVVALPITIIFNKFSKYYQRNKAMEGQNARSLTLSPCTVFLLQSDREISWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTN6</accession>
    <name>A0A673BTN6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">arf3a</name>
        <name evidence="16" type="synonym">arf3b</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BTN6"/>
    <dbReference type="SMR" id="A0A673BTN6"/>
    <dbReference type="Ensembl" id="ENSSORT00005046281.1">
        <property type="protein sequence ID" value="ENSSORP00005045145.1"/>
        <property type="gene ID" value="ENSSORG00005020737.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059363.1">
        <property type="protein sequence ID" value="ENSSORP00005058041.1"/>
        <property type="gene ID" value="ENSSORG00005025658.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="181" mass="20601" checksum="D6FA234DFAED3E5F" modified="2020-06-17" version="1">MGNIFGNLLKSLIGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLANQLKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BX20</accession>
    <name>A0A673BX20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">galnt17</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Golgi apparatus membrane</location>
            <topology evidence="2 18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_030008845.1">
        <property type="nucleotide sequence ID" value="XM_030152985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BX20"/>
    <dbReference type="FunCoup" id="A0A673BX20">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047494.1">
        <property type="protein sequence ID" value="ENSSORP00005046335.1"/>
        <property type="gene ID" value="ENSSORG00005021220.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432146"/>
    <dbReference type="CTD" id="64409"/>
    <dbReference type="InParanoid" id="A0A673BX20"/>
    <dbReference type="OrthoDB" id="9924649at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23438">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000017">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000012">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF38">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 18" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="20">
        <location>
            <begin position="466"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="599" mass="68148" checksum="5579FA563DED029F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BX59</accession>
    <name>A0A673BX59_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Kirre like nephrin family adhesion molecule 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">kirrel3b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BX59"/>
    <dbReference type="FunCoup" id="A0A673BX59">
        <property type="interactions" value="576"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046328.1">
        <property type="protein sequence ID" value="ENSSORP00005045192.1"/>
        <property type="gene ID" value="ENSSORG00005020756.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BX59"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05759">
        <property type="entry name" value="IgI_2_KIRREL3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05898">
        <property type="entry name" value="IgI_5_KIRREL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000094">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000103">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000170">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF49">
        <property type="entry name" value="KIN OF IRRE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045192.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="745" mass="81311" checksum="8FBB1B7644B3F5D9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXQ6</accession>
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        <submittedName>
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        <name evidence="12" type="primary">sh3rf2</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="854" mass="92679" checksum="C2514D1AA6AEDA7C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0N0</accession>
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        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 4</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="primary">LOC115422955</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="10">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
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        <text evidence="2 10">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A, COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="3 10">Belongs to the cytochrome c oxidase IV family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
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    </dbReference>
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        <property type="entry name" value="Cytochrome c oxidase subunit 4 isoform 1"/>
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        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyt_c_oxidase_su4"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
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        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01873">
        <property type="entry name" value="CYTCOXIDASE4"/>
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    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
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    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="177" mass="20090" checksum="301AD08B578F7AA0" modified="2020-06-17" version="1">MLRLTAGRVGSLLTRRATAALSTSSARMASHGHEVSETVDMSQPLYWDRLDTPLPDRPFKDIVSAADKSLKQKEKGPWSQLTKEEKIALYRLMFCQTYAEMKKPSSEWKTVMGGIFIFLGFTGLVVWWQSIYGKSIILFLVFFRLQTKPDTMTRHGTKSRAVNKINSGSVAFKLHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4G0</accession>
    <name>A0A673C4G0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein O/MICOS complex subunit Mic27 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C4G0"/>
    <dbReference type="FunCoup" id="A0A673C4G0">
        <property type="interactions" value="576"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048990.1">
        <property type="protein sequence ID" value="ENSSORP00005047807.1"/>
        <property type="gene ID" value="ENSSORG00005021823.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C4G0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019166">
        <property type="entry name" value="MIC26/MIC27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033182">
        <property type="entry name" value="MIC26/MIC27_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14564">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC26 / MIC27 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09769">
        <property type="entry name" value="ApoO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="204" mass="21806" checksum="29D0843CB392A1AC" modified="2025-10-08" version="2">MTLAGGVTGRAMPGALTLSSFTVFAAAGDGDKAETAATPLTASVDELSLYTVPQRKAHFVEREAGQLEQTVAALRRFVEPCASWCQSTYGRVRPRVQGAVRFGSDTYAYLKNPPADFYPRAGVIGFTGILGLFVARGSRVKQLLYPAGLMAVSASLYYPEQAAAIARSAGDSVYERTVQGYAAVEKLIRPQQDTKKGTDSETKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4L9</accession>
    <name>A0A673C4L9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115414853</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A673C4L9"/>
    <dbReference type="Ensembl" id="ENSSORT00005051525.1">
        <property type="protein sequence ID" value="ENSSORP00005050316.1"/>
        <property type="gene ID" value="ENSSORG00005022815.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C4L9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000705">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF3">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="10">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="333"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="155"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="572" mass="63652" checksum="B1723D751746694C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5J2</accession>
    <name>A0A673C5J2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C5J2"/>
    <dbReference type="Ensembl" id="ENSSORT00005048718.1">
        <property type="protein sequence ID" value="ENSSORP00005047538.1"/>
        <property type="gene ID" value="ENSSORG00005021742.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C5J2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF296">
        <property type="entry name" value="PROTOCADHERIN 1 GAMMA 11 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025691262" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="28"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="681"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="855"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="130"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="239"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="342"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="447"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="571"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="880" mass="97223" checksum="49D256DAB254AF2A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5Z1</accession>
    <name>A0A673C5Z1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Striatin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">strn3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Calmodulin-binding scaffolding protein which is the center of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Tetramerizes. Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26. The STRIPAK complex can be extended by adapter proteins such as SLMAP:SIKE1 or CTTNBP2NL. Interacts with CDC42BPB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat striatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C5Z1"/>
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        <property type="protein sequence ID" value="ENSSORP00005050861.1"/>
        <property type="gene ID" value="ENSSORG00005022995.1"/>
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    <dbReference type="InParanoid" id="A0A673C5Z1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070016">
        <property type="term" value="F:armadillo repeat domain binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.300:FF:000001">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000058">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000110">
        <property type="entry name" value="striatin-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000134">
        <property type="entry name" value="striatin-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF3">
        <property type="entry name" value="STRIATIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="18">
        <location>
            <begin position="69"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="416"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="469"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="522"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="658"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="227"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="88"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="736" mass="80629" checksum="C0DB5778CEBFE489" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7L0</accession>
    <name>A0A673C7L0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C7L0"/>
    <dbReference type="Ensembl" id="ENSSORT00005049383.1">
        <property type="protein sequence ID" value="ENSSORP00005048193.1"/>
        <property type="gene ID" value="ENSSORG00005022002.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C7L0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000145">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="4"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="107" mass="11889" checksum="41805C770EE12301" modified="2020-06-17" version="1">MNSPSEIHYSSYFVIEQVAPCDARVKEGQDVVISAKVRGQPTPMVYWLKDGVTIKTAGRFHVRQTDDGSSEMSISSAQRSDAGLYVCKITNEYGLEQTECRLEVIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9E3</accession>
    <name>A0A673C9E3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cyclin-G2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115425838</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for the control of the cell cycle at the G2/M (mitosis) transition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029999496.1">
        <property type="nucleotide sequence ID" value="XM_030143636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C9E3"/>
    <dbReference type="Ensembl" id="ENSSORT00005051845.1">
        <property type="protein sequence ID" value="ENSSORP00005050630.1"/>
        <property type="gene ID" value="ENSSORG00005022945.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425838"/>
    <dbReference type="InParanoid" id="A0A673C9E3"/>
    <dbReference type="OrthoDB" id="769138at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000006">
        <property type="entry name" value="Cyclin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="56"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="290"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="334" mass="37234" checksum="A7BB0F7D420A3155" modified="2020-06-17" version="1">MRDIQGIDALLLKELKSCCAKEHNFLPKETGLKLMESASTESHNGVSAKCRDTRVEELWSLTSFFGYSTQTFVQAVNLLDRFLTIMKVQPKHLPCIGVSCLHISAKMVEEESNISPTHELIRISQSKFTVSDLCRMEKIISEKLSVQPKAVTALTFLHLYYSAFPPQSAGREEIPSIGRLEAQLKACLCRLAFSKAKPSVLALSLIAQEFEALQSINLLKIVQQFQRHLKISDSVLLHWKELVAKCMAQYCSSDCNKPDNKKLVWIVSRRTAQCLQANHFSVPCLPTIPEGSWDESESEDSCEDMSSGEDSPCGSPGSDGEGAFFPTAFLHSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CEF9</accession>
    <name>A0A673CEF9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of G-protein signaling 17-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rgs17</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CEF9"/>
    <dbReference type="Ensembl" id="ENSSORT00005055231.1">
        <property type="protein sequence ID" value="ENSSORP00005053961.1"/>
        <property type="gene ID" value="ENSSORG00005024222.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CEF9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF196">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="83"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="209" mass="24465" checksum="64F8FB67D864D0F7" modified="2020-06-17" version="1">MPRSVSGVEMRKRQAAHIEAPPQAPGQPRPNTCCLCWCGCCKCLWNEDRMERSERQTCTKMDSIEAVEEQHPSLDEVLSWARSFEMMLRSLEGREVFREFLRSEYSEDNLLFWLACEELKKETNPSVVDEKARIIYEDYVSILSPKEVSLDSRVREGINQTLAEPSNLMYEEAQLQIYTLMHRDSFPRFLNSSVYRDLLANRRRTCLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGF8</accession>
    <name>A0A673CGF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">rnf144aa</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes ube2l3 and ube2l6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RBR family. RNF144 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673CGF8"/>
    <dbReference type="FunCoup" id="A0A673CGF8">
        <property type="interactions" value="163"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055942.1">
        <property type="protein sequence ID" value="ENSSORP00005054666.1"/>
        <property type="gene ID" value="ENSSORG00005024465.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGF8"/>
    <dbReference type="OrthoDB" id="10009520at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16777">
        <property type="entry name" value="mRING-HC-C4C4_RBR_RNF144A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20352">
        <property type="entry name" value="Rcat_RBR_RNF144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000006">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000051">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="16"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="20"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="295" mass="33210" checksum="1644393404D78FCB" modified="2020-06-17" version="1">MTTARYRPTWELAVDPLVSCKLCLGEFPLEQMTTITQCQCVFCTLCLKQYVELLIKEGLETAISCPDSACPKRGHLQENEIECMVATEMMQRYKKLQFEREVLLDPCRTWCPSSTCQAVCQLKEADSPALPQLVQCAVCALEFCSACKANWHPGQACQENNLPITSFLPGENSSFYKNEEDDAPIKRCPKCKVYIERDEGCAQMMCKNCKHAFCWYCLESLDDDFLLIHYDKGPCRNKLGHSRASVIWHRTQVVGIFAGFGLLLLVASPFLLLATPIVLCCKCKCSKGDDDPLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CK81</accession>
    <name>A0A673CK81_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Si:ch73-21k16.5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gnpat2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="6">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CK81"/>
    <dbReference type="Ensembl" id="ENSSORT00005053725.1">
        <property type="protein sequence ID" value="ENSSORP00005052478.1"/>
        <property type="gene ID" value="ENSSORG00005023644.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CK81"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008611">
        <property type="term" value="P:ether lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF22">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000437">
        <property type="entry name" value="GPAT_DHAPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="143"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="633" mass="71581" checksum="F59C73D5004E9618" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRF9</accession>
    <name>A0A673CRF9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cellular nucleic acid-binding protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cnbpa</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CRF9"/>
    <dbReference type="Ensembl" id="ENSSORT00005055805.1">
        <property type="protein sequence ID" value="ENSSORP00005054531.1"/>
        <property type="gene ID" value="ENSSORG00005024418.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055807.1">
        <property type="protein sequence ID" value="ENSSORP00005054533.1"/>
        <property type="gene ID" value="ENSSORG00005024418.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427960at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000038">
        <property type="entry name" value="CCHC-type zinc finger, nucleic acid-binding protein a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000002">
        <property type="entry name" value="cellular nucleic acid-binding protein-like isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000026">
        <property type="entry name" value="cellular nucleic acid-binding protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025836">
        <property type="entry name" value="Zn_knuckle_CX2CX4HX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47103">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47103:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14392">
        <property type="entry name" value="zf-CCHC_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="167" mass="18354" checksum="9C781ABE78E35F47" modified="2020-06-17" version="1">MEMSSSNSECFGCGRSGHWVKHCPNAGGGRGRGRGRGRGKELFCYRCGDQGHMARDCDQTEDACYNCHRSGHISRDCKEPKKEREQLCYTCGKAGHMARDCDHANEQKCYSCGGFGHIQKLCDKVKCYRCGEIGHVAVHCSKASETNCYNCGKAGHLAKECTIEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSI4</accession>
    <name>A0A673CSI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gpr183a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CSI4"/>
    <dbReference type="FunCoup" id="A0A673CSI4">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057620.1">
        <property type="protein sequence ID" value="ENSSORP00005056324.1"/>
        <property type="gene ID" value="ENSSORG00005025069.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSI4"/>
    <dbReference type="OrthoDB" id="10021141at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15159">
        <property type="entry name" value="7tmA_EBI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF7">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="47"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="373" mass="41822" checksum="A719789CE238C077" modified="2020-06-17" version="1">MNSTTVPGTTTPSPNSTICNTLYAHRDYARVLMPLVYCVVFVVGLFGNCLALHVIRPNLKKMNSTTLYSLNLVISDILFTFSLPFRITYYAMGFHWPLGETLCKISGLIFYINTYAGVNFMTCLSIDRFIAVVLPLRFARLRKVSNVRYICVGVWLVVLAQTLPLLGMPMTKTEPEGFITCMEYPNFETVPHIATILIGAVFLGYVIPVVTILGCYSVLCSKLRISAKTNHLTEKSGRSRKAIGVICCVSLVFVICYSPYHIDILQYMIRKLVSSPSCVDLTAFQVSLHITVCLMNLNSCLDPFIYFFACKGYKRKLLKLLKLQVSMSFSSVARTSPEGSSKDIIDGNKFQLNSSTMGATSERLTERRLHKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CV59</accession>
    <name>A0A673CV59_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Matrix metalloproteinase-9</fullName>
            <ecNumber evidence="4">3.4.24.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">92 kDa gelatinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">92 kDa type IV collagenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Gelatinase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">mmp9</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of gelatin types I and V and collagen types IV and V.</text>
            <dbReference type="EC" id="3.4.24.35"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="27">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="27">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="27">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="27">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="24">Exists as monomer or homodimer; disulfide-linked. Also exists as heterodimer with LCN2. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.35" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673CV59"/>
    <dbReference type="Ensembl" id="ENSSORT00005058675.1">
        <property type="protein sequence ID" value="ENSSORP00005057359.1"/>
        <property type="gene ID" value="ENSSORG00005025438.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CV59"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000010">
        <property type="entry name" value="72 kDa type IV collagenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000011">
        <property type="entry name" value="Matrix metalloproteinase-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF30">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 27" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0177">Collagen degradation</keyword>
    <keyword evidence="18 29" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 32" id="KW-0732">Signal</keyword>
    <keyword evidence="13 26" id="KW-0862">Zinc</keyword>
    <keyword evidence="17" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="32">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025389039" description="Matrix metalloproteinase-9" evidence="32">
        <location>
            <begin position="22"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="33">
        <location>
            <begin position="227"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="33">
        <location>
            <begin position="285"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="33">
        <location>
            <begin position="343"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="30">
        <location>
            <begin position="515"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="30">
        <location>
            <begin position="561"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="30">
        <location>
            <begin position="607"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="463"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="28">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="31">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="active site" evidence="25">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="27">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="519"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="521"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="565"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="613"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="661"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="290"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="29">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001425"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00033338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00062173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="706" mass="79776" checksum="5B1A56F1B0DFE645" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CWR4</accession>
    <name>A0A673CWR4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.86</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">inpp5d</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.86" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673CWR4"/>
    <dbReference type="Ensembl" id="ENSSORT00005057719.1">
        <property type="protein sequence ID" value="ENSSORP00005056423.1"/>
        <property type="gene ID" value="ENSSORG00005025105.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CWR4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045779">
        <property type="term" value="P:negative regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045659">
        <property type="term" value="P:negative regulation of neutrophil differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045579">
        <property type="term" value="P:positive regulation of B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10343">
        <property type="entry name" value="SH2_SHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000035">
        <property type="entry name" value="phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000005">
        <property type="entry name" value="phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057509">
        <property type="entry name" value="C2_SHIP1-2_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057510">
        <property type="entry name" value="C2_SHIP1-2_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051:SF3">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 5-PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24147">
        <property type="entry name" value="C2_SHIP1-2_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24150">
        <property type="entry name" value="C2_SHIP1-2_first"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="7"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="106"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="914"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="994"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1090" mass="123339" checksum="8CAF247ACED4CD2D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CYI1</accession>
    <name>A0A673CYI1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">slc8b1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029999837.1">
        <property type="nucleotide sequence ID" value="XM_030143977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CYI1"/>
    <dbReference type="Ensembl" id="ENSSORT00005059238.1">
        <property type="protein sequence ID" value="ENSSORP00005057917.1"/>
        <property type="gene ID" value="ENSSORG00005025615.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426044"/>
    <dbReference type="CTD" id="80024"/>
    <dbReference type="InParanoid" id="A0A673CYI1"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099093">
        <property type="term" value="P:calcium export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025559773" description="Sodium/calcium exchanger membrane region domain-containing protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="496"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="535"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="108"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="432"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="592" mass="64723" checksum="7B037DC191F3A039" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y2Q3</accession>
    <name>A0A672Y2Q3_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y2Q3"/>
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    </dbReference>
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        <location>
            <begin position="82"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="237"/>
        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="25387" checksum="50FAFEBEF9B7E19B" modified="2020-06-17" version="1">ILFIYCCTCSYEGRIYKYGDTVYDTHDGNGTCFTAVCGDGGNIKRTIETCRTVTTTQKPPTTSFVFTTSGKNERVIHFKIVQPRSTSTGSTTSMTSTLPPSTTTSGTEKPTTTTQVTGKSTTIIIPTETPYTPTETTEKPITTAEVTEESTTTTKTIETSATTSKVTEETTTTTETTENPTVTTEVTEKWTTTTPEVATGTVTSTPPPTRSQVATTTTKTVTTVQWSSTSKGSTTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y445</accession>
    <name>A0A672Y445_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">Sushi domain-containing protein</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y445"/>
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        <property type="protein sequence ID" value="ENSSORP00005000673.1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Complement Module, domain 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
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        <location>
            <begin position="19"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="19"/>
            <end position="92"/>
        </location>
    </feature>
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            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="204" mass="23028" checksum="8727AE84A6F267F1" modified="2020-06-17" version="1">MVFLITKITSVLTSIAMALYCQNSAFRHFMFSFPSVVNGARSVRPEAGFAVGTVVRFSCNQGYQLEGPSQISCHGRDTGTPKWSDRSPKCVCKSLSSLKYDPCPNPGVPDNGYQTLYKHSYQAGETLRFFCYEGYELIGEVIISCVPGHPSQWNSPPPFCKGKVRTYRIVLIIEHKIYYLDLKTSFRTGRYIIKKLCPLSLHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4H2</accession>
    <name>A0A672Y4H2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Neuropilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">nrp2a</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the neuropilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y4H2"/>
    <dbReference type="Ensembl" id="ENSSORT00005001257.1">
        <property type="protein sequence ID" value="ENSSORP00005001222.1"/>
        <property type="gene ID" value="ENSSORG00005000233.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4H2"/>
    <dbReference type="OrthoDB" id="6155811at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005021">
        <property type="term" value="F:vascular endothelial growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000013">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000010">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014648">
        <property type="entry name" value="Neuropilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022579">
        <property type="entry name" value="Neuropilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
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    <keyword evidence="4" id="KW-0358">Heparin-binding</keyword>
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    <keyword evidence="7 23" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <position position="198"/>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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        <location>
            <position position="212"/>
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        <ligand>
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        <location>
            <position position="253"/>
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        <ligand>
            <name>Ca(2+)</name>
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="915" mass="101673" checksum="FE85609D1ACFFC68" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y569</accession>
    <name>A0A672Y569_9TELE</name>
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        <recommendedName>
            <fullName evidence="21">Geranylgeranyl pyrophosphate synthase</fullName>
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            <ecNumber evidence="7">2.5.1.10</ecNumber>
            <ecNumber evidence="6">2.5.1.29</ecNumber>
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            <fullName evidence="16">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Farnesyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Geranylgeranyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
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        <name evidence="23" type="primary">ggps1</name>
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    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>isopentenyl diphosphate + (2E)-geranyl diphosphate = (2E,6E)-farnesyl diphosphate + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:33019"/>
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            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>isopentenyl diphosphate + dimethylallyl diphosphate = (2E)-geranyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22408"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:58057"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homohexamer; trimer of homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the FPP/GGPP synthase family.</text>
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        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
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    </dbReference>
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    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0808">Transferase</keyword>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00062555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00072971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="332" mass="38457" checksum="EC074B43CC8C29AA" modified="2020-06-17" version="1">PNRRQVSLEKEISSQRTSWLNKAASERILLEPYKYLLQLPGKQVRTKLAQAFNHWLNVPEDKLQVIIEVTEMLHNASLLIDDIEDSSKLRRGFPVAHSIYGVPSVINSANYVYFLGLEKVLTLEHPEAVRVFTRQLLELHRGQGLDIHWRDTYTCPTEQEYRHMVLQKTGGLFGLAVGLMQLFSDWKRDLKPLLDALGLFFQIRDDYANLSSQEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENVDVKRYCVDYLEKVGSFAYTRRTLRDLEAEAYRLIRELGGNPQLESLVKLLSQMHREAEAQAQAQAADHNQNQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8H3</accession>
    <name>A0A672Y8H3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Urotensin-2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Urotensin II receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115429590</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">High affinity receptor for urotensin-2 and urotensin-2B. The activity of this receptor is mediated by a G-protein that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y8H3"/>
    <dbReference type="Ensembl" id="ENSSORT00005000044.1">
        <property type="protein sequence ID" value="ENSSORP00005000044.1"/>
        <property type="gene ID" value="ENSSORG00005000026.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8H3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001604">
        <property type="term" value="F:urotensin II receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097746">
        <property type="term" value="P:blood vessel diameter maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14999">
        <property type="entry name" value="7tmA_UII-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000670">
        <property type="entry name" value="Urot_II_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230:SF123">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00647">
        <property type="entry name" value="UROTENSIN2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="280" mass="31422" checksum="E7553D006E755010" modified="2020-06-17" version="1">MYVYIINLALADLLYLSTIPFVVCTYFAHDWLFGEAGCRILLSLDLLTMHASVFILVAMSAERYRAVARPFSAHRSSSRGRKLVAGTIWGLAFMLTLPMMVMIRLREGKPTVAGLVKRICFPTWTPEAFKTYITVLFFTSVLLPGLIIVGLYTGLARRYWAAQATLGGSSRSARRRGLKQKVISMVFSIVVAYWACFLPFWGWQLAKLFSPESLRALSPAAHNYVNFFVTCLTYGNSCINPFLYTLLTRNYKDYLAQKGQSVMSSRVDLGSAVSTPLQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y936</accession>
    <name>A0A672Y936_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y936"/>
    <dbReference type="Ensembl" id="ENSSORT00005003422.1">
        <property type="protein sequence ID" value="ENSSORP00005003323.1"/>
        <property type="gene ID" value="ENSSORG00005002027.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y936"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256:SF65">
        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025675878" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="15"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="161" mass="17766" checksum="CCE38A6EE74F4D91" modified="2020-06-17" version="1">IGSISVCLSSLHLFPGVSGVTVVTQKPPAVTVEKGETVTMDCNLGSVTGSNCYWYKQSPGGVPQFILVYYHSWGSPSYGSGFSSPKFNSNHQSRSDYRLIINNVQEGDSAVYYCKTWDSSASLLLFGSFTFSEHELITDKAARVWLTHVCLCHQLYIYISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCC2</accession>
    <name>A0A672YCC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YCC2"/>
    <dbReference type="Ensembl" id="ENSSORT00005000343.1">
        <property type="protein sequence ID" value="ENSSORP00005000321.1"/>
        <property type="gene ID" value="ENSSORG00005000235.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCC2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001532">
        <property type="entry name" value="Immunoglobulin heavy variable 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="100" mass="11407" checksum="86F16FD187C2CB8F" modified="2020-06-17" version="1">QTLTESEPAVKRPGESHRLTCTTSGFTFSSYDMHWVRQAPGKGLEWIAEITYSGGSTYYSQSFQGRFTISRDNGRNQLYLQMNSLKTEDSAVYYCARETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YEU7</accession>
    <name>A0A672YEU7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dusp14</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030008746.1">
        <property type="nucleotide sequence ID" value="XM_030152886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YEU7"/>
    <dbReference type="Ensembl" id="ENSSORT00005002092.1">
        <property type="protein sequence ID" value="ENSSORP00005002036.1"/>
        <property type="gene ID" value="ENSSORG00005001259.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432082"/>
    <dbReference type="CTD" id="11072"/>
    <dbReference type="InParanoid" id="A0A672YEU7"/>
    <dbReference type="OrthoDB" id="285418at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14572">
        <property type="entry name" value="DUSP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000049">
        <property type="entry name" value="Dual specificity protein phosphatase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020420">
        <property type="entry name" value="Atypical_DUSP_subfamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052103">
        <property type="entry name" value="Dual_spec_Phospatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45961:SF5">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45961">
        <property type="entry name" value="IP21249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01910">
        <property type="entry name" value="ADSPHPHTASEB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="7">
        <location>
            <begin position="41"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="8">
        <location>
            <begin position="103"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="213" mass="23977" checksum="6D723C1F60B5CFC5" modified="2020-06-17" version="1">MGSRSQGFFTNHHHHHHHHRSSMVPATVPRLLPENGSLLGGIAQITPNLFLSRGNVASNRSLLLSKGITCVVNATIELPNFNWPHMEYVKVPLADMPHSPISLYFDSVADKIHSVGRKRGAVLVHCAAGVSRSASLCLAYLMKYHRVSLAEAHAWVKARRPVIRPNGGFWRQLIEYERKLFGRNSVKMVQTPYGVIPDVYERDRRSLAPYWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFA5</accession>
    <name>A0A672YFA5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Tripartite motif-containing protein 16-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115436963</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030015853.1">
        <property type="nucleotide sequence ID" value="XM_030159993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YFA5"/>
    <dbReference type="Ensembl" id="ENSSORT00005002472.1">
        <property type="protein sequence ID" value="ENSSORP00005002411.1"/>
        <property type="gene ID" value="ENSSORG00005001434.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436963"/>
    <dbReference type="InParanoid" id="A0A672YFA5"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="15"/>
            <end position="58"/>
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            <begin position="248"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="558" mass="63593" checksum="C9D6F79F0FA57DAC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YG87</accession>
    <name>A0A672YG87_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial tRNA methylthiotransferase CDK5RAP1</fullName>
            <ecNumber evidence="8">2.8.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CDK5 activator-binding protein C42</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">CDK5 regulatory subunit-associated protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">mt-tRNA-2-methylthio-N6-dimethylallyladenosine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">mt-tRNA-N6-(dimethylallyl)adenosine(37) methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">cdk5rap1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37067"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="Rhea" id="RHEA-COMP:10376"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="ChEBI" id="CHEBI:74417"/>
            <dbReference type="EC" id="2.8.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:37068"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005003578.1"/>
        <property type="gene ID" value="ENSSORG00005002143.1"/>
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    <dbReference type="InParanoid" id="A0A672YG87"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0035597">
        <property type="term" value="F:tRNA-2-methylthio-N(6)-dimethylallyladenosine(37) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
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        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01864">
        <property type="entry name" value="tRNA_metthiotr_MiaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
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    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
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    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
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    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
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    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
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    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01574">
        <property type="entry name" value="miaB-methiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020:SF2">
        <property type="entry name" value="MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00413">
        <property type="entry name" value="CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="16">
        <location>
            <begin position="96"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="17">
        <location>
            <begin position="240"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="15">
        <location>
            <begin position="497"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="601" mass="67636" checksum="5F344175746FE0CC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHU7</accession>
    <name>A0A672YHU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FA complementation group G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fancg</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030000059.1">
        <property type="nucleotide sequence ID" value="XM_030144199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YHU7"/>
    <dbReference type="Ensembl" id="ENSSORT00005004258.1">
        <property type="protein sequence ID" value="ENSSORP00005004136.1"/>
        <property type="gene ID" value="ENSSORG00005002524.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426195"/>
    <dbReference type="CTD" id="2189"/>
    <dbReference type="InParanoid" id="A0A672YHU7"/>
    <dbReference type="OrthoDB" id="6355951at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039684">
        <property type="entry name" value="FANCG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15254:SF2">
        <property type="entry name" value="FANCONI ANEMIA GROUP G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15254">
        <property type="entry name" value="FANCONI ANEMIA GROUP G PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="622" mass="70053" checksum="E83B2889CFA2F3BB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YII2</accession>
    <name>A0A672YII2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Exosome complex component MTR3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Exosome component 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">mRNA transport regulator 3 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">exosc6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Non-catalytic component of the RNA exosome complex which has 3'-&gt;5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the RNA exosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029991433.1">
        <property type="nucleotide sequence ID" value="XM_030135573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YII2"/>
    <dbReference type="FunCoup" id="A0A672YII2">
        <property type="interactions" value="771"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005004527.1">
        <property type="protein sequence ID" value="ENSSORP00005004395.1"/>
        <property type="gene ID" value="ENSSORG00005002665.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420337"/>
    <dbReference type="CTD" id="118460"/>
    <dbReference type="InParanoid" id="A0A672YII2"/>
    <dbReference type="OrthoDB" id="2504340at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11371">
        <property type="entry name" value="RNase_PH_MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000035">
        <property type="entry name" value="Exosome complex component MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="14">
        <location>
            <begin position="45"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="274" mass="29615" checksum="57233178812430A7" modified="2020-06-17" version="1">MPTDTKRVRGPEVSQSPSVFQCKPAAALPSCGVRADGRQRDQLDVRPVFVRCGLVSQAKGSAYIEAGNTKLMCCVYGPRETKRKDETDMKCGRLTADMRFAPFSCPERGSWIQSSQDKDFSLMLLESLQPAVCLHKYPRSQIEVNVMVLENSGSVLAHAITCASLALADAGIEMYDLVLGCSIRQDGASYVVDPTYVEENSCGSASEENQGGLTVAFLPSLNQISGLQSDGEMTKESLTDGIRTCIEGCYKLYPVVQQALAKAVRKAAPPPSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ42</accession>
    <name>A0A672YJ42_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJ42"/>
    <dbReference type="Ensembl" id="ENSSORT00005000387.1">
        <property type="protein sequence ID" value="ENSSORP00005000363.1"/>
        <property type="gene ID" value="ENSSORG00005000267.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005004727.1">
        <property type="protein sequence ID" value="ENSSORP00005004591.1"/>
        <property type="gene ID" value="ENSSORG00005002791.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001594">
        <property type="entry name" value="Immunoglobulin heavy variable 9-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627676" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="31"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="117" mass="13158" checksum="D8654C2DFA4AA82B" modified="2020-06-17" version="1">MRTFLVFAVLVLTGVQGWSITQSDPVVMRPGESHRLTCTTSGLLFSISWMGWIRQAPGKGLERIATDRSDGGTNYYSQSVQGRFTTSRDNSKEQVYLQMNSLKTEDSAVYYCARHPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ66</accession>
    <name>A0A672YJ66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJ66"/>
    <dbReference type="Ensembl" id="ENSSORT00005004776.1">
        <property type="protein sequence ID" value="ENSSORP00005004640.1"/>
        <property type="gene ID" value="ENSSORG00005002823.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJ66"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001878">
        <property type="entry name" value="Immunoglobulin heavy variable 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="11"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="121" mass="14160" checksum="CE0C86495FA1ACB1" modified="2020-06-17" version="1">EMQFLIVMRPGESHRLTCTATGLSFSSYWMGWIRQPPGKGLEWIAIIRYDSSYIYYSQSVQGRFTISRDNGRNQLYLQMNSLKTEDSAVYYCARDPQCLELVKQLYKILKNDSVVVTLYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKW6</accession>
    <name>A0A672YKW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger and BTB domain-containing protein 7A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Leukemia/lymphoma-related factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YKW6"/>
    <dbReference type="Ensembl" id="ENSSORT00005005303.1">
        <property type="protein sequence ID" value="ENSSORP00005005152.1"/>
        <property type="gene ID" value="ENSSORG00005003090.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YKW6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18326">
        <property type="entry name" value="BTB_POZ_ZBTB7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001448">
        <property type="entry name" value="Zinc finger and BTB domain containing 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000115">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000138">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF6">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="61"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="504"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="216"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="381"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="524"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="393"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="603" mass="64754" checksum="FCE50CC3C6AB9880" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YL06</accession>
    <name>A0A672YL06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dual-specificity kinase</fullName>
            <ecNumber evidence="4">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">tesk1b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A672YL06"/>
    <dbReference type="Ensembl" id="ENSSORT00005005427.1">
        <property type="protein sequence ID" value="ENSSORP00005005271.1"/>
        <property type="gene ID" value="ENSSORG00005003146.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YL06"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14155">
        <property type="entry name" value="PKc_TESK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000202">
        <property type="entry name" value="Dual specificity testis-specific protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000134">
        <property type="entry name" value="Dual specificity testis-specific protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF3">
        <property type="entry name" value="DUAL SPECIFICITY TESTIS-SPECIFIC PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="65"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="319"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="405"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="659"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="495"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="511"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="583"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="673"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="696" mass="75523" checksum="DAED3DB46A922A65" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YP38</accession>
    <name>A0A672YP38_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115421971</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Synthesis and degradation of fructose 2,6-bisphosphate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YP38"/>
    <dbReference type="Ensembl" id="ENSSORT00005006561.1">
        <property type="protein sequence ID" value="ENSSORP00005006308.1"/>
        <property type="gene ID" value="ENSSORG00005000303.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YP38"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043540">
        <property type="term" value="C:6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000001">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000047">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF15">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="13">
        <location>
            <begin position="33"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="11">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="252"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="483" mass="56147" checksum="09309127196EEDF4" modified="2020-06-17" version="1">EVRANVVYMNDVSHVQHLQQQPLTHFLCLCFCNSPTMIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGQYRREAVKTYKNFEFFKPDNEEAMRIRKACASAALKDVSAYFTKEHGQVAVFDATNTTRERRAIIISFAKERGYKVFFVESICEDPEIIAENIKQVKFGSPDYVDRDIDEAMEDFIQRIECYRANYMSIDDEKDRKLSYIKIFDVGSRYLVNRVQDHIQSRIVYYLMNIHVTPRSIYLSRHGESELNLSGRIGGDAGLSSRGQKYANALATFMRGQTIKDLKVWTSHMKRTIQTAEALGVQYEQWKALNEIDAGVCEEMTYEEIQENFPEEFALRDQDKYRYRYPKGESYEDLVHRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKPADELPYLRCPLHTVLKLTPVAYGKWLAHKVSGLCNSFPDSLTFFFTNVGCKVESFFLNIEAVNTHRERPVVSFRKYTVHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YP70</accession>
    <name>A0A672YP70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 18">Telomerase reverse transcriptase</fullName>
            <ecNumber evidence="2 18">2.7.7.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 18">Telomerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">tert</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16 18">Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 18">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.49"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Catalytic subunit of the telomerase holoenzyme complex composed minimally of TERT and the telomerase RNA template component (TERC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Chromosome</location>
            <location evidence="18">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 18">Belongs to the reverse transcriptase family. Telomerase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="2 18"/>
    <dbReference type="RefSeq" id="XP_030002325.1">
        <property type="nucleotide sequence ID" value="XM_030146465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YP70"/>
    <dbReference type="Ensembl" id="ENSSORT00005006598.1">
        <property type="protein sequence ID" value="ENSSORP00005006343.1"/>
        <property type="gene ID" value="ENSSORG00005003224.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427797"/>
    <dbReference type="CTD" id="7015"/>
    <dbReference type="InParanoid" id="A0A672YP70"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000333">
        <property type="term" value="C:telomerase catalytic core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022616">
        <property type="term" value="P:DNA strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01648">
        <property type="entry name" value="TERT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.70:FF:000002">
        <property type="entry name" value="Telomerase reverse transcriptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.90:FF:000001">
        <property type="entry name" value="Telomerase reverse transcriptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021891">
        <property type="entry name" value="Telomerase_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003545">
        <property type="entry name" value="Telomerase_RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049139">
        <property type="entry name" value="TERT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066:SF0">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12009">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21399">
        <property type="entry name" value="TERT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01365">
        <property type="entry name" value="TELOMERASERT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00975">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 18" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11 18" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="10 18" id="KW-0779">Telomere</keyword>
    <keyword evidence="5 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="20">
        <location>
            <begin position="582"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="197"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU365061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1104" mass="126232" checksum="017EC87B3930312A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPJ7</accession>
    <name>A0A672YPJ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pfdn6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002272.1">
        <property type="nucleotide sequence ID" value="XM_030146412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YPJ7"/>
    <dbReference type="FunCoup" id="A0A672YPJ7">
        <property type="interactions" value="2132"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006740.1">
        <property type="protein sequence ID" value="ENSSORP00005006483.1"/>
        <property type="gene ID" value="ENSSORG00005003805.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427717"/>
    <dbReference type="CTD" id="10471"/>
    <dbReference type="InParanoid" id="A0A672YPJ7"/>
    <dbReference type="OrthoDB" id="248120at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein folding"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="80"/>
            <end position="114"/>
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            <dbReference type="ARBA" id="ARBA00008045"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="126" mass="14562" checksum="859ABAC90D7856C2" modified="2020-06-17" version="1">MAEAIQKKLKAELEKYTQMQKDVSKSMSARQKLETQLTENNIVKEEMDLLDSTNTVYKLIGPVLVKQDLEEAKSTVAKRLEYINGEIQRYETLLKDMEKKSEQHREVLSSLQQEFQRAQGLTVGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQP5</accession>
    <name>A0A672YQP5_9TELE</name>
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        <submittedName>
            <fullName evidence="4">Patched domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ptchd1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:synapse"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:chemical synaptic transmission"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
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        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
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    <dbReference type="InterPro" id="IPR051697">
        <property type="entry name" value="Patched_domain-protein"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796:SF36">
        <property type="entry name" value="PATCHED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
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        <location>
            <begin position="55"/>
            <end position="75"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="516" mass="58050" checksum="654DD0910C922B60" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YX55</accession>
    <name>A0A672YX55_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Eukaryotic translation elongation factor 1 delta b (guanine nucleotide exchange protein)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eef1db</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YX55"/>
    <dbReference type="Ensembl" id="ENSSORT00005009494.1">
        <property type="protein sequence ID" value="ENSSORP00005009188.1"/>
        <property type="gene ID" value="ENSSORG00005005044.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YX55"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
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        <property type="entry name" value="EF-1_beta_acid_region_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
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    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF86">
        <property type="entry name" value="ELONGATION FACTOR 1-DELTA ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10587">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01182">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="8">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="7">
        <location>
            <begin position="209"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="66"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="135"/>
            <end position="172"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU003791"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
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        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="32820" checksum="F76CFACD42BB3FCB" modified="2020-06-17" version="1">MSALDCLAQEKIWFEKPRYDEAERRFYERMNGYVQAPQDVGANTILQDIARARENIQKSLAGLKNTLSNRGSAQPSPSLPSHITTSSTNSTADQGELISRIKSLELENQSLHKVVDDLRTALSKLECRVAVLEKSPAPAPPAPTPSVPYTNGTTVQQKSSAPVKKEEEDEDDFDLFGSDEEDEEAERIKEERLKAYAEKKAKKPALIAKSSILLDVKPWDDETDMVKLEECVRSVQADGLLWGTSKLVPVGYGIKKLQIACVVEDDKVGTDMLEEEITKFEDYVQSVDVAAFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXU7</accession>
    <name>A0A672YXU7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cortexin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ctxn1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013892"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="862" mass="96657" checksum="5C7574C7C9EB5EBB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZK9</accession>
    <name>A0A672YZK9_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Alpha and gamma adaptin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">aagab</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029992230.1">
        <property type="nucleotide sequence ID" value="XM_030136370.1"/>
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    <dbReference type="Ensembl" id="ENSSORT00005010569.1">
        <property type="protein sequence ID" value="ENSSORP00005010236.1"/>
        <property type="gene ID" value="ENSSORG00005005548.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420827"/>
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    <dbReference type="InParanoid" id="A0A672YZK9"/>
    <dbReference type="OrthoDB" id="10261384at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019341">
        <property type="entry name" value="Alpha/Gamma-adaptin-bd_p34"/>
    </dbReference>
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        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Adaptin_binding"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35671" checksum="7FC3269F3DAD3904" modified="2020-06-17" version="1">MSATEETTTMTIPCVLVTSSDSGFKEEELIKQILNLNTLPEPTKREEAVSWYPWTINNKYYTADVRLCVVPSTFQMTSEIAQSMQAFIAYFDSSVKDGLEKLHPWISVIEDIAPEVLILVCDKVCENGVTRHEAQQWCLAHAFELVELNPQELPDEDDDFPESTGVKRIVQALNANVWTSVEMKDGHNQGLGLMSSLVACRHNNPRSCQDPPSSSSPVGGTLVNEEDDQKENSTHSSTQEDTVVDAMTDLDIQELANLTAGDADVDNFERLFTKLKEMKDKASSLPHEQRKVHAEKVAKAFWMAIGGDEDEIDGLSSGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z032</accession>
    <name>A0A672Z032_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Vav 3 guanine nucleotide exchange factor b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115437782</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z032"/>
    <dbReference type="Ensembl" id="ENSSORT00005010772.1">
        <property type="protein sequence ID" value="ENSSORP00005010436.1"/>
        <property type="gene ID" value="ENSSORG00005005631.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z032"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01223">
        <property type="entry name" value="PH_Vav"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000024">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037832">
        <property type="entry name" value="PH_Vav"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818:SF5">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR VAV3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="16">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="15">
        <location>
            <begin position="222"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="382"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="495"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="562"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="640"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="764"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="834" mass="96521" checksum="D6567DD5F75D0C7E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2D2</accession>
    <name>A0A672Z2D2_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z2D2"/>
    <dbReference type="Ensembl" id="ENSSORT00005011138.1">
        <property type="protein sequence ID" value="ENSSORP00005010767.1"/>
        <property type="gene ID" value="ENSSORG00005005842.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2D2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="61" mass="6703" checksum="6668390BAF4F0818" modified="2020-06-17" version="1">MVDPVFLNKPPLFFLQTVNGVSGSHISTPKSNKSSTPSPTSPRTPEIDELESVMRHHVQSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3G0</accession>
    <name>A0A672Z3G0_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Glucose-induced degradation protein 4 homolog</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">gid4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GID4/VID24 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A672Z3G0"/>
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        <property type="protein sequence ID" value="ENSSORP00005011274.1"/>
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        <property type="component" value="Chromosome 8"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GID4/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534:SF3">
        <property type="entry name" value="GID COMPLEX SUBUNIT 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 24"/>
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    </dbReference>
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        <property type="entry name" value="Vac_ImportDeg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="229" mass="26326" checksum="52F162B01C33922C" modified="2020-06-17" version="1">MTVADGDTLALIMPVRAECCCSPDSACPSSASLVPPAPINAHQPGVATTLLYSGSQFRGYQKSKGNAYDVEVVLQHVTVEDSYLCGYLKIKGLTEEYPTLTTFFAGEIISRKRPFLTRKWDADEDVDRKHWGKFQPFYKYAKTFNSDDFDYEALDSSDYVFMRWKEQFLVPDHTIKDISGASFAGFYYICFQKSTATIEGYYYHRSSEWYQSLNLNHVRERSMPIYEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3I7</accession>
    <name>A0A672Z3I7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z3I7"/>
    <dbReference type="Ensembl" id="ENSSORT00005011746.1">
        <property type="protein sequence ID" value="ENSSORP00005011365.1"/>
        <property type="gene ID" value="ENSSORG00005006092.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3I7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025483681" description="Immunoglobulin V-set domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="3">
        <location>
            <begin position="32"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="139" mass="15313" checksum="C032C5EEFA920058" modified="2020-06-17" version="1">MTLVSVLIWTLLCCCQVTVTQPGAVSSDLGASTTISCTTSQNVAGFNELAWYQQRDGETPKLLIYYATRRQSGISSRFSGSGSNSYFTLTISDVQAEDAAVVMGLVQRLNPNARPERKGYSRSGSRRGSKHKVTRWRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9P2</accession>
    <name>A0A672Z9P2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Retinoic acid receptor RXR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nuclear receptor subfamily 2 group B member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rxrba</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for retinoic acid that acts as a transcription factor. Forms homo- or heterodimers with retinoic acid receptors (rars) and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate gene expression in various biological processes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Heterodimer; with a rar molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z9P2"/>
    <dbReference type="FunCoup" id="A0A672Z9P2">
        <property type="interactions" value="942"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013620.1">
        <property type="protein sequence ID" value="ENSSORP00005013212.1"/>
        <property type="gene ID" value="ENSSORG00005006232.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9P2"/>
    <dbReference type="OrthoDB" id="5873264at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06956">
        <property type="entry name" value="NR_DBD_RXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06943">
        <property type="entry name" value="NR_LBD_RXR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000005">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000052">
        <property type="entry name" value="Ultraspiracle nuclear receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021780">
        <property type="entry name" value="Nuc_recep-AF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000003">
        <property type="entry name" value="Retinoid-X_rcpt/HNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11825">
        <property type="entry name" value="Nuc_recep-AF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00545">
        <property type="entry name" value="RETINOIDXR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="124"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="225"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="470" mass="51495" checksum="860D3B6B745CD59C" modified="2020-06-17" version="1">MGDSRDSRSPDSSSVSSPPSGQRSPPLVPSAAASMTSLPPITAAGVNSPISSIGSPFSVISSSLGSPCLPGTPSVGYGPISSPQINSTVSMSGLHAVSSSDDVKPPLGLKQLSSHSPGPMLSQKRMCSICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLTYTCRDNKDCMVDKRQRNRCQYCRYQKCLAMGMKREVAKMNDRSVQEERQRNKDREGEVESTSAVNEEMPVEKILEAEMAVEQKTELHTDGSSGGSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSISVKDGILLATGLHVHRNSAHSAGVGAIFDRAHNAEVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIILFNPDAKGLSNPSEVELLRERVYASLEAYCKQKYPDQQGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZA62</accession>
    <name>A0A672ZA62_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="5">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115415526</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZA62"/>
    <dbReference type="Ensembl" id="ENSSORT00005013810.1">
        <property type="protein sequence ID" value="ENSSORP00005013400.1"/>
        <property type="gene ID" value="ENSSORG00005006931.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013813.1">
        <property type="protein sequence ID" value="ENSSORP00005013403.1"/>
        <property type="gene ID" value="ENSSORG00005006931.1"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF0">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="231" mass="25789" checksum="491166B8E17B6280" modified="2020-06-17" version="1">MALLYGLLWRLLLLLVHVNRTVVCWLRVRARSFQGRLWERAVAALLLPVALIGFPGQQKKLNRNPDGTVYPVPGARAAAAGRRSRWVCDGRSVEKLPVHIGLLVAEEEPDYTDIANLVVWCMAVGISYVSVYDDNGIFQKNNCRLLEEIVRQQQDLLGTEGSKYNVETSDPVGSLLTWLGLYTGKNMDVNPENPPNDICTCVTRKDAHDVFNLIIIFRLPHFAAAGVHVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZB00</accession>
    <name>A0A672ZB00_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Kinesin family member 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">kif1ca</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZB00"/>
    <dbReference type="Ensembl" id="ENSSORT00005014101.1">
        <property type="protein sequence ID" value="ENSSORP00005013688.1"/>
        <property type="gene ID" value="ENSSORG00005007040.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZB00"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1C ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="5"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="744"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="929"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="778"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="982"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1016"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1051"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1170" mass="130556" checksum="420B6EF749A6950F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZF27</accession>
    <name>A0A672ZF27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZF27"/>
    <dbReference type="Ensembl" id="ENSSORT00005015915.1">
        <property type="protein sequence ID" value="ENSSORP00005015424.1"/>
        <property type="gene ID" value="ENSSORG00005007826.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZF27"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="Homeobox_CS"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016362">
        <property type="entry name" value="TF_POU_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF125">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002629">
        <property type="entry name" value="Transcription_factor_POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="14">
        <location>
            <begin position="236"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="326"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="328"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
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        <location>
            <begin position="98"/>
            <end position="146"/>
        </location>
    </feature>
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        <location>
            <begin position="197"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="425" mass="46210" checksum="279383A0E9AE4326" modified="2020-06-17" version="1">MATATSSPYLASSRILSSGSVLHSDRRGGMQPGSTAVTTVSSGYRGDPPVKMVQSDFMQGAMVASNGGHMLSHAHQWVTSLPHAAAAAAAAAVAAAEAGSPWPPGSQAQEGKRNAGREDLHSGSALHHRSAHLVPHQTHPGSWGSASAAHISIAEGQQQQQQQQHQSLIYSQPGGFTVNGMLSSHSGQSLMHSGLVRGESPELDHGSHHHHHHHHHNHHTHHHQHHGVSHEPHSDEDTPTSDDLEHFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSTTGSPTSIDKIATQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEINSLADSLQLEKEVVRVWFCNRRQKEKRMTPPGLPRTPEDAYSQVGSMGPDTPSPSIDCKRISKCCRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFQ0</accession>
    <name>A0A672ZFQ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zgc:77784</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSSORP00005015654.1"/>
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            <begin position="25"/>
            <end position="121"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="137" mass="15434" checksum="5EC2A5A6654A3C8D" modified="2020-06-17" version="1">SSCGPHLTPWRAVYVLFSRFEDSAPRIVEDPSDLIVSKGEPATLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYTCVARNYLGEAISRNASLEVASKLRPGFGPKFKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGV0</accession>
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        <recommendedName>
            <fullName evidence="1">FDX-ACB domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="2" type="primary">fdxacb1</name>
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        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Apogoninae</taxon>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <end position="647"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="647" mass="73006" checksum="1D3728EB11DF11D6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHI7</accession>
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        <submittedName>
            <fullName evidence="6">Small G protein signaling modulator 1b</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="primary">sgsm1b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the RUTBC family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A672ZHI7"/>
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        <property type="gene ID" value="ENSSORG00005008386.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZHI7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR22957">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RUN"/>
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    </dbReference>
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        <property type="entry name" value="TBC"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
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        <location>
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            <end position="857"/>
        </location>
    </feature>
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        <location>
            <begin position="585"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
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            <end position="632"/>
        </location>
    </feature>
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        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034124"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50826"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="924" mass="104185" checksum="65B794ABFFD1FDF7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI58</accession>
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        <submittedName>
            <fullName evidence="10">Neuronal pentraxin receptor b</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="primary">LOC115438285</name>
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    <organism evidence="10 11">
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZI58"/>
    <dbReference type="Ensembl" id="ENSSORT00005017323.1">
        <property type="protein sequence ID" value="ENSSORP00005016810.1"/>
        <property type="gene ID" value="ENSSORG00005008493.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZI58"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000012">
        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF162">
        <property type="entry name" value="NEURONAL PENTRAXIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pentraxin (PTX)" evidence="9">
        <location>
            <begin position="335"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="278"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="365"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="548" mass="59831" checksum="C1F679A84A56B258" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI79</accession>
    <name>A0A672ZI79_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nup85</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
            <location evidence="1 10">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029996402.1">
        <property type="nucleotide sequence ID" value="XM_030140542.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A672ZI79">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017429.1">
        <property type="protein sequence ID" value="ENSSORP00005016916.1"/>
        <property type="gene ID" value="ENSSORG00005007049.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423632"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="C:nuclear pore outer ring"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of nuclear pore"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mRNA export from nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein import into nucleus"/>
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    </dbReference>
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        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8 10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="652" mass="74507" checksum="BFAC4E646B691180" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZK66</accession>
    <name>A0A672ZK66_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZK66"/>
    <dbReference type="Ensembl" id="ENSSORT00005018074.1">
        <property type="protein sequence ID" value="ENSSORP00005017555.1"/>
        <property type="gene ID" value="ENSSORG00005008767.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZK66"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098858">
        <property type="term" value="C:actin-based cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028457">
        <property type="entry name" value="ABI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460:SF60">
        <property type="entry name" value="ABI GENE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460">
        <property type="entry name" value="ABL INTERACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="107" mass="11883" checksum="02D60B529E0B92C8" modified="2020-06-17" version="1">MTEQKNFAEVIAQIFKEAPAARKDLADNQSNLLRVADYCENNYLQDAQTIDEAKALATQALASVTYQINSLASTLLRLLDSQAMQVKDMESSVNLLSLVRLTSTLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLL8</accession>
    <name>A0A672ZLL8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZLL8"/>
    <dbReference type="FunCoup" id="A0A672ZLL8">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018209.1">
        <property type="protein sequence ID" value="ENSSORP00005017689.1"/>
        <property type="gene ID" value="ENSSORG00005008805.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLL8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="313" mass="35993" checksum="B19158221C18577B" modified="2020-06-17" version="1">MGNSTHLTHFILSAYFDSRLVKYIFVMILMTLYVLMLVSNVLLIVVICVNRSLHEPMYLFLCSLFVNELFGSTGIFPSLLIRILRGIHTVSTSLCFLQIYCIYSYVIVEFFTLSVMSYDRYFAICHPLQYNTHMSRKRISVIIIVPWFYGFLYVSVMISIMSSLHICENINKASCDNYPIVNLACSDTTTVNIYGLTIAFITVSFPTFLVLYSYMRILKVCFSGSKQTRHKAVSTCTPHLASLINFSFGCLFEIITSRFNINHVPNILRVFLSLYFLIIQPLFNPLMYGLNISNIRICCRKLLFGGEAKFTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZN85</accession>
    <name>A0A672ZN85_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">poly(ADP-ribose) glycohydrolase</fullName>
            <ecNumber evidence="2">3.2.1.143</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pargl</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the poly(ADP-ribose) glycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZN85"/>
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        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019179.1">
        <property type="protein sequence ID" value="ENSSORP00005018631.1"/>
        <property type="gene ID" value="ENSSORG00005009183.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZN85"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA repair"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048362">
        <property type="entry name" value="PARG_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF9">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20811">
        <property type="entry name" value="PARG_cat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="PARG catalytic Macro" evidence="6">
        <location>
            <begin position="335"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="591" mass="66806" checksum="8918791F8C6A5EB8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQS1</accession>
    <name>A0A672ZQS1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium uptake protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">micu1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the MICU1 family. MICU1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030012720.1">
        <property type="nucleotide sequence ID" value="XM_030156860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZQS1"/>
    <dbReference type="FunCoup" id="A0A672ZQS1">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019292.1">
        <property type="protein sequence ID" value="ENSSORP00005018743.1"/>
        <property type="gene ID" value="ENSSORG00005008854.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434751"/>
    <dbReference type="CTD" id="10367"/>
    <dbReference type="InParanoid" id="A0A672ZQS1"/>
    <dbReference type="OrthoDB" id="10056860at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061891">
        <property type="term" value="F:calcium ion sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903852">
        <property type="term" value="P:positive regulation of cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000088">
        <property type="entry name" value="Calcium uptake protein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000159">
        <property type="entry name" value="Calcium uptake protein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF1">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="240"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="431"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="64"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="499" mass="56393" checksum="4404E6D8FB3263DF" modified="2020-06-17" version="1">MMFRLRALSAATVGLVQLSRRYHSGAVRGSGKRRLMLAALAGVTGVSASAGLLWKRAYADAGPSVQHSEQQGREEAEFVRDADSETESEKSVEASSGDEESKDEGGDGKKKKPRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVIGEHGDAEVYMTPQDFIRSITPNEKQPENLGLDQFIVKRYDGKNEERCLLHFVEKIAQEREKFADEDSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEIAFKMFDLNGDGEVDLEEFEQVQSIIRSQTSMGMRHRDRSTTGNTLKTAGCSSALTTYFFGEDLKGKLTIGAFLEFQRKLQHDVLKLEFERNDPVDGRIAERQFGGMLLAYSGVQSRKLKLMQKGLKKMFKDAQGITFEEVENFFTFLKNVNDVDTALSFYHMAGASIDKATMKQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFIAIMKQRLMRGLEKPKDMGFTRLVRAMWKCAQDTAWDFATPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVM2</accession>
    <name>A0A672ZVM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 93</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ccdc93</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC93 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029980906.1">
        <property type="nucleotide sequence ID" value="XM_030125046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZVM2"/>
    <dbReference type="Ensembl" id="ENSSORT00005021030.1">
        <property type="protein sequence ID" value="ENSSORP00005020457.1"/>
        <property type="gene ID" value="ENSSORG00005007890.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412498"/>
    <dbReference type="CTD" id="54520"/>
    <dbReference type="InParanoid" id="A0A672ZVM2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039116">
        <property type="entry name" value="CCDC93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019159">
        <property type="entry name" value="CCDC93_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048747">
        <property type="entry name" value="CCDC93_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16441:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16441">
        <property type="entry name" value="FIDIPIDINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09762">
        <property type="entry name" value="CCDC93_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21673">
        <property type="entry name" value="CCDC93_N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC93 N-terminal" evidence="7">
        <location>
            <begin position="37"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="CCDC93 coiled-coil" evidence="6">
        <location>
            <begin position="190"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="563"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="635" mass="73504" checksum="3ABAB20C8391F5D7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZW20</accession>
    <name>A0A672ZW20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase</fullName>
            <ecNumber evidence="1">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115433699</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="1"/>
    <dbReference type="RefSeq" id="XP_030011017.1">
        <property type="nucleotide sequence ID" value="XM_030155157.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030011018.1">
        <property type="nucleotide sequence ID" value="XM_030155158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZW20"/>
    <dbReference type="Ensembl" id="ENSSORT00005022136.1">
        <property type="protein sequence ID" value="ENSSORP00005021490.1"/>
        <property type="gene ID" value="ENSSORG00005010519.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433699"/>
    <dbReference type="InParanoid" id="A0A672ZW20"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025347894" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="30"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="263"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="567" mass="65790" checksum="8B0511D32E1D5A92" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZYF3</accession>
    <name>A0A672ZYF3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115422274</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2. Seems to recognize and bind to exposed hydrophobic regions in target proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZYF3"/>
    <dbReference type="FunCoup" id="A0A672ZYF3">
        <property type="interactions" value="1003"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022762.1">
        <property type="protein sequence ID" value="ENSSORP00005022106.1"/>
        <property type="gene ID" value="ENSSORG00005010769.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZYF3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904154">
        <property type="term" value="P:positive regulation of retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000015">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF6">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="500"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="558" mass="61986" checksum="33388346CE9509F9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZ58</accession>
    <name>A0A672ZZ58_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="5">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">rps3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="5"/>
    <dbReference type="RefSeq" id="XP_030007974.1">
        <property type="nucleotide sequence ID" value="XM_030152114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZZ58"/>
    <dbReference type="Ensembl" id="ENSSORT00005023161.1">
        <property type="protein sequence ID" value="ENSSORP00005022500.1"/>
        <property type="gene ID" value="ENSSORG00005010901.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431619"/>
    <dbReference type="CTD" id="6188"/>
    <dbReference type="InParanoid" id="A0A672ZZ58"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001235">
        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000005">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10 17" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="9 16" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="19">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="200"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="246" mass="27010" checksum="D11AE8FC884E713B" modified="2020-06-17" version="1">MAVQISKKRKFVSDGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPSGKIGPKKPLPDHVSIVEPKEETLPTTPVSEQKGCPKPEVPVMPQGTPVPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZF4</accession>
    <name>A0A672ZZF4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZZF4"/>
    <dbReference type="Ensembl" id="ENSSORT00005023260.1">
        <property type="protein sequence ID" value="ENSSORP00005022595.1"/>
        <property type="gene ID" value="ENSSORG00005010986.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZF4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071797">
        <property type="term" value="C:LUBAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990450">
        <property type="term" value="F:linear polyubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026254">
        <property type="entry name" value="RNF31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032065">
        <property type="entry name" value="RNF31-UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057426">
        <property type="entry name" value="RNF31_UBA_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16004">
        <property type="entry name" value="RING FINGER PROTEIN 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16678">
        <property type="entry name" value="UBA_HOIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25163">
        <property type="entry name" value="UBA_RNF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="591" mass="66173" checksum="96656BD8C5BC02F2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0A1</accession>
    <name>A0A673A0A1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bryoporin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115423406</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nematocyst</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029996031.1">
        <property type="nucleotide sequence ID" value="XM_030140171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A0A1"/>
    <dbReference type="Ensembl" id="ENSSORT00005022648.1">
        <property type="protein sequence ID" value="ENSSORP00005021993.1"/>
        <property type="gene ID" value="ENSSORG00005010742.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423406"/>
    <dbReference type="InParanoid" id="A0A673A0A1"/>
    <dbReference type="OrthoDB" id="6132998at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042151">
        <property type="term" value="C:nematocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051715">
        <property type="term" value="P:cytolysis in another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:monoatomic cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046931">
        <property type="term" value="P:pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.270.20">
        <property type="entry name" value="Cytolysin/lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050677">
        <property type="entry name" value="Actinoporin_PFT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009104">
        <property type="entry name" value="Anemon_actinoporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015926">
        <property type="entry name" value="Cytolysin/lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40388">
        <property type="entry name" value="BRYOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40388:SF1">
        <property type="entry name" value="BRYOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06369">
        <property type="entry name" value="Anemone_cytotox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63724">
        <property type="entry name" value="Cytolysin/lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0166">Nematocyst</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="4" id="KW-1053">Target membrane</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025625140" evidence="6">
        <location>
            <begin position="22"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="188" mass="20123" checksum="FCA1E8FD70A318F4" modified="2020-06-17" version="1">MAKAAGAAAAALGAVVAAGEAIAGASPTHRQCNVEITNVCKDYSLSNPRMYLDSGHCAIPQSPFIDSGTSDVAVFVKTPNTACGSVGVITYDLQDNKTQQSTKKMAVMFSVPYDFNFYSNVYAVGIFDHSRECDKTLYNEMISGSGSFARKKAKEPGSLIYEGGTFTIMATMSDTYQPVIKLRISQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A468</accession>
    <name>A0A673A468_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Neuronal PAS domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">npas2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A468"/>
    <dbReference type="FunCoup" id="A0A673A468">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024801.1">
        <property type="protein sequence ID" value="ENSSORP00005024091.1"/>
        <property type="gene ID" value="ENSSORG00005011606.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A468"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990513">
        <property type="term" value="C:CLOCK-BMAL transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19736">
        <property type="entry name" value="bHLH-PAS_PASD1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Circadian locomoter output cycles protein"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46055">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NCTRNSLOCATR"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0090">Biological rhythms</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="88"/>
        </location>
    </feature>
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        <location>
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            <end position="181"/>
        </location>
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            <end position="336"/>
        </location>
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            <begin position="1"/>
            <end position="48"/>
        </location>
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        </location>
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            <begin position="580"/>
            <end position="657"/>
        </location>
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        <location>
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        </location>
    </feature>
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            <end position="572"/>
        </location>
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            <end position="46"/>
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            <end position="603"/>
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        </source>
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        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="867" mass="98280" checksum="DB3EBD11B093647E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6X7</accession>
    <name>A0A673A6X7_9TELE</name>
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        <submittedName>
            <fullName evidence="13">Cytochrome P450 2J2-like</fullName>
        </submittedName>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <name>heme</name>
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        </cofactor>
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        <subcellularLocation>
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        </subcellularLocation>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <location>
            <position position="447"/>
        </location>
        <ligand>
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        </ligand>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="502" mass="57586" checksum="F0CEBB394A03F953" modified="2020-06-17" version="1">MTELENVLMDSLFSGFFVNLDATALLLFIVVFILTADYIKNRRLTGFPPGPTALPIVGNIFTLDHKRTHESMTELAGRYGDVYSLRMGQRWVVVLNGFEAVTQALVTQGDSFSDRPGLPLHDDTFHGLVFSNGNTWKQQRRFALSTLKYFGFGKKSLEPVILEEFTYVAKEISAYKGKPFNPHLIVNNTVANIICLLVFGHRFEYGDEQFKKLMKLFEQSFEAQVSVSAELYNAFPKLMRRLPGPHQKFMRIIEELKDFVRTEVKDHKQKWDPSDLRDYIDYNLNEIQTDDSTFDEECLIMSVLDLFAAGSETTSTTLRWALLFMVKYPEIQERVQAEIDSVIGQSRPPSVDDRADLPYTDAVIHEVQRMGNIVPLNLPHVTNRDVHLRGYTIPKGVTIIPNLTSVLFDKREWKTPFTFNPGHFLNEEGKFVKPAAFIPFSAGKRLCLGENLARMELFLFFTSFMQRFTFSMPPGVKPVLDYRFGITLSPVHYEICAKQRHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A7F5</accession>
    <name>A0A673A7F5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase TRIM21-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115423973</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029996888.1">
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</entry>
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    </evidence>
    <sequence length="338" mass="38004" checksum="1292914C6FB45D68" modified="2020-06-17" version="1">MNSSLDPLNHSSTDPSNLSAPFCLLEIGYSQIFNTCLLEVSIILLLTVLIISGNLVVIFVFHCAPLLSHHTTSAFIQTMAYADLLVGVSCLFPSMSLLHHLQGLDPKLTCQVFGYMVSVLKSVSMASLACVSVDRYIAITRPLTYASLVTPCRVRCCIILIWLYSALVFLPSFLGWGKPGYHGDVVEWCAVEWRTRPVFTTFIVALLYAPAALTVCFTYANIFKICRQHTREISERHQPSSTYPDKRYAMVLFRITSVFYILWLPYILYFLLESGGIYHHPAASFLTTWLAISNSFCNCLIYSLSNSAFRKGLKRLCSFCLQSSGDANECIRSWLFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AC03</accession>
    <name>A0A673AC03_9TELE</name>
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        <recommendedName>
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        </recommendedName>
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        <name evidence="9" type="synonym">urm1</name>
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            <taxon>Actinopterygii</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the URM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AC03"/>
    <dbReference type="FunCoup" id="A0A673AC03">
        <property type="interactions" value="1497"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027511.1">
        <property type="protein sequence ID" value="ENSSORP00005026729.1"/>
        <property type="gene ID" value="ENSSORG00005012804.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AC03"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01764">
        <property type="entry name" value="Ubl_Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000021">
        <property type="entry name" value="Ubiquitin-related modifier 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03048">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14986">
        <property type="entry name" value="RURM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037379">
        <property type="entry name" value="Ubiquitin-related_modifier_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025475986" description="Ubiquitin-related modifier 1" evidence="8">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="modified residue" description="1-thioglycine" evidence="6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)" evidence="6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="103" mass="11849" checksum="58C5AD7228D294F0" modified="2020-06-17" version="1">MYAKICLIFLFLSRGGAELLFKGVKEHHVTLPTQSEPWDVKQLLVWIQRNLLKERPELFVQGDSVRPGILVLINDADWELMGELDYQLQDQDNIVFISTLHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGT4</accession>
    <name>A0A673AGT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AGT4"/>
    <dbReference type="Ensembl" id="ENSSORT00005029278.1">
        <property type="protein sequence ID" value="ENSSORP00005028469.1"/>
        <property type="gene ID" value="ENSSORG00005013625.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGT4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052227">
        <property type="entry name" value="Arf-Rho-GAP_ANK-PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45899:SF1">
        <property type="entry name" value="ARF-GAP WITH RHO-GAP DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45899">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 15B, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="170"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="368"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="3">
        <location>
            <begin position="586"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="775" mass="87573" checksum="0DA7A4F98667370B" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGT6</accession>
    <name>A0A673AGT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AGT6"/>
    <dbReference type="Ensembl" id="ENSSORT00005029733.1">
        <property type="protein sequence ID" value="ENSSORP00005028915.1"/>
        <property type="gene ID" value="ENSSORG00005013805.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGT6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF1">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="32229" checksum="D757DDE23AC91194" modified="2020-06-17" version="1">DLHTLLGKYESSKHMFVFSSDESEYLQSVRGFEPFQPHDDEKNEDQHIRILLHGPVGHGKSSFINSVNSVLQGRMTGRALADACGKSFTKKYKTYKITKEEDGTFYPFVFNDIMGLEKDQGVCEEDIKLAMMGHVRDGYTVKDDPYYNTNPSLSDKAHVLVCVVSANEVHLLNHDAMKKMRNVRLEASDMGIPQMAILTNIDEVCPEVNADLRNVYKSQFVKKTGSKSLGIPLNYIFPVKNYHSEITPNDDLEMLILMALRQIINFGKDFARPLPLHHTICNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHP1</accession>
    <name>A0A673AHP1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane protein 120B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tmem120b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for efficient adipogenesis. Does not show ion channel activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM120 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AHP1"/>
    <dbReference type="FunCoup" id="A0A673AHP1">
        <property type="interactions" value="747"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029615.1">
        <property type="protein sequence ID" value="ENSSORP00005028799.1"/>
        <property type="gene ID" value="ENSSORG00005013761.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AHP1"/>
    <dbReference type="OrthoDB" id="2015098at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012926">
        <property type="entry name" value="TMEM120A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 120B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07851">
        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="338" mass="40087" checksum="A0EB73E8CADFBDE8" modified="2020-06-17" version="1">MSKPKFQLEWEEIDAEYQQLQESHKIYRQKLEELTNLQATCSNAISKQRKCLKDLGYSLNKCAKTCDAKDLKLITDIQTQVKDKENVFFDMEAYLPKKNGLYLNLVLGNVNVTLLSNQAKFAYKDEYEKFKLYMTIILMFGAITCLFFLNCRVTDEIFNFLLVWYYCTLTIRESILMSNGSRIKGWWVSHHYVSTFLSGVMLTWPEGPMYQKFRSQFLAFSIYQSFVQFLQYYYQSGCLYRLRALGERNQLDLTVEGFQSWMWRGLTFLLPFLFFGHFWQLYNSVTLFHLAGHEDCKEWQVFMLALTFLVLFLGNFLTTLKVVHQKIQKNKEKVQKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIG2</accession>
    <name>A0A673AIG2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">T-box transcription factor T-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115428155</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002862.1">
        <property type="nucleotide sequence ID" value="XM_030147002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AIG2"/>
    <dbReference type="FunCoup" id="A0A673AIG2">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030343.1">
        <property type="protein sequence ID" value="ENSSORP00005029510.1"/>
        <property type="gene ID" value="ENSSORG00005014095.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428155"/>
    <dbReference type="InParanoid" id="A0A673AIG2"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20192">
        <property type="entry name" value="T-box_TBXT_TBX19-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000002">
        <property type="entry name" value="T-box transcription factor Brachyury"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002070">
        <property type="entry name" value="TF_Brachyury"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF169">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR T-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00938">
        <property type="entry name" value="BRACHYURY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="7">
        <location>
            <begin position="40"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="422" mass="45839" checksum="8B0F87CAE628F296" modified="2020-06-17" version="1">MTSSSNPDQRLEHLLSAVESEFQKGSEKGDASERDIKLTLEDADLWNKFKELTNEMIVTKTGRRMFPVLRASVTGLDPNAMYSVLLDFVAADNNRWKYVNGEWVPGGKPEPQSPSCVYIHPDSPNFGAHWMKAPVSFSKVKLSNKLNGGGQIMLNSLHKYEPRIHIVKVGGIQKMISSQSFPETQFIAVTAYQNEEITALKIKHNPFAKAFLDAKERSDHKEVPDHSADNQQSGYSQLGGWFLPGQTPICPSSSPPQFSGTAGHSSGSYCERYSSLRSHRAAPYPSHYPHRTSTTNNYMDNTSGTLPTHDSWSALQIPNSTGMGTLSHTTNSTTNSSQYPSLWSVAGTTLTPSGSASGSIPGGLTSQFLRGSSYTGLTSSLPVSSPSSMYDPSLSEVGVGEAQFESSIARLAASWAPVAQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKL0</accession>
    <name>A0A673AKL0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Olfactomedin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115416107</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AKL0"/>
    <dbReference type="Ensembl" id="ENSSORT00005030029.1">
        <property type="protein sequence ID" value="ENSSORP00005029198.1"/>
        <property type="gene ID" value="ENSSORG00005013938.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AKL0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022082">
        <property type="entry name" value="Noelin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF34">
        <property type="entry name" value="NOELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12308">
        <property type="entry name" value="Noelin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="10">
        <location>
            <begin position="138"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="139"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="393" mass="44893" checksum="E37F7555D60CA0FF" modified="2020-06-17" version="1">ESCAKDGSGTDPVQNMSQSIEVLDQRTQRDLQFVEKMEVQLKSLENKFRAVEEGHETNLARQYKSIKAKMEELRPLIPVLESYKADALLVRQFKEEAANVTELLGGLQEQLGGLDYQDLHTRVTSLEERLRACMQRLACGKLTGIAEPITIKTSGSRFGSWMTDPVAPPGDNRVWYMDGYHNNRFVREYQSMYDFMTTDNFTSHRLPHPWSGTGQVVYNGSIYFNKFQSHTIIKFDFGTSLISRSRQLDFAGYNNMYHYSWGGHSDIDLMVDEGGLWAVYATNQNAGNIVISQLNPNTLQIVRSWTTNHPKRSAGEAFMICGTLYVTNGYSGGTKVYYAYSTNSSTYEYIDIPLTNKYSHLSMLDYNPRDRALYAWNNGHQVLYNVTLYHIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AL84</accession>
    <name>A0A673AL84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AL84"/>
    <dbReference type="Ensembl" id="ENSSORT00005030851.1">
        <property type="protein sequence ID" value="ENSSORP00005030009.1"/>
        <property type="gene ID" value="ENSSORG00005014333.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AL84"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025509863" description="UPAR/Ly6 domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="2">
        <location>
            <begin position="18"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="93" mass="10212" checksum="BF3C3927256C5CAA" modified="2020-06-17" version="1">MKAFAVALLLLVAFTYGQALQCQNCVREAPDSGNCVETTEMCPPEMDACAKITYPAPYENTFHKSCFKMGECLKLAITQGLMVSCCNWDGCNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQU8</accession>
    <name>A0A673AQU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">chymotrypsin</fullName>
            <ecNumber evidence="9">3.4.21.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115416954</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.1" evidence="9"/>
    <dbReference type="RefSeq" id="XP_029986706.1">
        <property type="nucleotide sequence ID" value="XM_030130846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AQU8"/>
    <dbReference type="FunCoup" id="A0A673AQU8">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032925.1">
        <property type="protein sequence ID" value="ENSSORP00005032040.1"/>
        <property type="gene ID" value="ENSSORG00005015219.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416954"/>
    <dbReference type="InParanoid" id="A0A673AQU8"/>
    <dbReference type="OrthoDB" id="5918597at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000176">
        <property type="entry name" value="Chymotrypsinogen A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000181">
        <property type="entry name" value="Chymotrypsinogen A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250">
        <property type="entry name" value="CHYMOTRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250:SF54">
        <property type="entry name" value="CHYMOTRYPSINOGEN B2 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025589082" description="chymotrypsin" evidence="11">
        <location>
            <begin position="19"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="34"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="264" mass="28245" checksum="3AADE84EF78DE0F2" modified="2020-06-17" version="1">MAFLWIVSCLAFVSAAYGCGVPAIPPQVTGYARIVNGEEAVPHSWPWQVSLQQSNGFHFCGGSLINENWVVTAAHCNVRTYHRVVAGEHNKGYGSNEVVQVLKPAKVFTHPKWNPRTINNDISLIKLSTPARLGTNVSPVCLAESSDVFAPGMTCVTSGWGLTRYNAPSTPNLLQQAALPLLSNEQCKRHWGSNISDVMICAGGAGATSCMGDSGGPLVCEKDNAWTLVGIVSWGSSRCSTSTPAVYARVTELRGWVDQILAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQV0</accession>
    <name>A0A673AQV0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AQV0"/>
    <dbReference type="Ensembl" id="ENSSORT00005031712.1">
        <property type="protein sequence ID" value="ENSSORP00005030848.1"/>
        <property type="gene ID" value="ENSSORG00005014726.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQV0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="243" mass="24763" checksum="0BB9DF31B9E620DC" modified="2020-06-17" version="1">STVCDPSVCDPSVCDPSTVCDPSTVCDPAVCDPSTVCDPAVCDPSTVCDPSVCDPSTVCDPSVCDPSAVCDPSTVCDPAVCDPSKVCDPSVCDPSVCDPSTVCDPSTVCDPTVCDPSTVCDPAVCDPSTVCDPSVCDPSVCDPSTVCDPSVCHPSTVCDPSVCDPSTVCDPSAVCDPSTVCDPSVCHPSTVCDPSVFCDPSVCDPSTVCDPSTDSSFPRFFPKKFSSEFCFGFISLACVSTDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARJ5</accession>
    <name>A0A673ARJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L34</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ARJ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005031862.1">
        <property type="protein sequence ID" value="ENSSORP00005030997.1"/>
        <property type="gene ID" value="ENSSORG00005014783.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARJ5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46595">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="126" mass="14431" checksum="A6A0FEC79F0AD7E6" modified="2020-06-17" version="1">MKYILNHFKKVQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGICPGRLHGIPAVRPQVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQAQSQKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUE7</accession>
    <name>A0A673AUE7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ras-related protein Rab-5B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rab5b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AUE7"/>
    <dbReference type="Ensembl" id="ENSSORT00005032878.1">
        <property type="protein sequence ID" value="ENSSORP00005031993.1"/>
        <property type="gene ID" value="ENSSORG00005015195.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUE7"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000995">
        <property type="entry name" value="RAB5B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="216" mass="23754" checksum="2AE6891F6865FCE1" modified="2020-06-17" version="1">MSSRGSGGRSNGTLPQTKICQFKLVLLGDMAVGKSSLVLRFVKGQFDEFQETTIGAAFLAQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVFDITKPETFERAKAWVKELQRQASPNIVIALAGNKADLAEKRLVEYEEAQTYAEDTGLLFMETSAKTAMNVNELFLAIAKKMPKTDTQNPTHAARHRGVNLQDPDAHSTRACCGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUY1</accession>
    <name>A0A673AUY1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">slc1a7a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AUY1"/>
    <dbReference type="FunCoup" id="A0A673AUY1">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033044.1">
        <property type="protein sequence ID" value="ENSSORP00005032157.1"/>
        <property type="gene ID" value="ENSSORG00005015282.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUY1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF22">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00714">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="321"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="535"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="563" mass="62063" checksum="9C4CF1E5DFB96721" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AV97</accession>
    <name>A0A673AV97_9TELE</name>
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        <submittedName>
            <fullName evidence="14">Homeobox protein Hox-D12a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hoxd12a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AV97"/>
    <dbReference type="FunCoup" id="A0A673AV97">
        <property type="interactions" value="146"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005032277.1"/>
        <property type="gene ID" value="ENSSORG00005015317.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AV97"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46440:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46440">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="200"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
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            <begin position="202"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="117"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
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            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
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            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="270" mass="30030" checksum="D6FCE9DCE19649DF" modified="2020-06-17" version="1">MEMCERNPLNPGYVGSLLNFAPPESLYFPNLRGNGAHIPGLHQIPYNRREVCTLPWTSSSSCTSGPAAAPPPPPQSSRAFGGYCPPFLSNSVSVNTNSSGGHAKAHLEESARCFQDTNHKREESGRQDEVYAGEHGAAGDRGYSDLNRSHGSAAQLGPDSAGPLNVNGTKQEQNLNHPSSSNTCSRTTFAEGAPWCSSQVKTRKKRKPYSKPQLAELENEFMMNEFINRQKRKELSDRLDLSDQQVKIWFQNRRMKKKRLMLREQAFSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWR6</accession>
    <name>A0A673AWR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coiled-coil SMC6 And NSE5 INteracting (CANIN) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM178 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AWR6"/>
    <dbReference type="Ensembl" id="ENSSORT00005034622.1">
        <property type="protein sequence ID" value="ENSSORP00005033709.1"/>
        <property type="gene ID" value="ENSSORG00005015959.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AWR6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044276">
        <property type="entry name" value="CANIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026161">
        <property type="entry name" value="FAM178"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16046">
        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16046:SF9">
        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14816">
        <property type="entry name" value="CANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coiled-coil SMC6 And NSE5 INteracting (CANIN)" evidence="3">
        <location>
            <begin position="594"/>
            <end position="963"/>
        </location>
    </feature>
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        <location>
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            <end position="68"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        </location>
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        <location>
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            <end position="1170"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14816"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1224" mass="137693" checksum="754C1336E2C37E54" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYW3</accession>
    <name>A0A673AYW3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AYW3"/>
    <dbReference type="Ensembl" id="ENSSORT00005035950.1">
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        <property type="gene ID" value="ENSSORG00005016515.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AYW3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027812">
        <property type="entry name" value="DUF4653"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35673">
        <property type="entry name" value="UPF0500 PROTEIN C1ORF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35673:SF1">
        <property type="entry name" value="UPF0500 PROTEIN C1ORF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15546">
        <property type="entry name" value="DUF4653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="357" mass="39895" checksum="BE20DE8A69E9A35F" modified="2020-06-17" version="1">MLHHQDQTLNSSYGGKGGLESSSSLQNQKGDKDGNFNFLPGKDDEGFTGNGGDENRNQVRPRNLGPLGRDPPNSSPSSGFHGGVMSPRSRLPCWSPLEPLPEIECGDDGYGRVPPDGAEERRMEEDEARMMMFQNEVKQMEKMEQHRRKSSLGLRRRDEGEDERKLEEDDEWGDSDSESEFSYRSGGSASSLSMECGPEGMLMGGWDRIGVGEPKSNQQEGDLNRNRDAAGRRSFSRRSLTGSNLGNVLEEGAEEQDDDQSSDSDGELPELMDAVWTLRDRERFKAQEMEKHQVQLTMYRRLALIRWVRTLQSRVQEQQNRLQSSFDVILTHRKELLRMGAAAAVANAVPAAAVGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZS2</accession>
    <name>A0A673AZS2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Recoverin</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AZS2"/>
    <dbReference type="Ensembl" id="ENSSORT00005035566.1">
        <property type="protein sequence ID" value="ENSSORP00005034644.1"/>
        <property type="gene ID" value="ENSSORG00005016329.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZS2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF166">
        <property type="entry name" value="VISININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="174" mass="20321" checksum="09C5C39840207C00" modified="2020-06-17" version="1">MGNSKSGAMSKEILEDLKLNTKFSESELVQWYENFKKQCPSGRISKEEFQNIYTKFSRVRRQHLRPARVPIIALHMTSTGKTTRKLEWAFSLFDVDKNGYITKSEVKEICHFQADSKEDLSTLPEDENTPEKRADKLWKIFNKEDNDRVAEGEFIQGVLDNDDALRLIQYQPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0K6</accession>
    <name>A0A673B0K6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Gamma-aminobutyric acid type A receptor gamma1 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">gabrg1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B0K6"/>
    <dbReference type="Ensembl" id="ENSSORT00005034973.1">
        <property type="protein sequence ID" value="ENSSORP00005034058.1"/>
        <property type="gene ID" value="ENSSORG00005016032.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B0K6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19054">
        <property type="entry name" value="LGIC_TM_GABAAR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000003">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit gamma-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005437">
        <property type="entry name" value="GABRG-1/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01620">
        <property type="entry name" value="GABAARGAMMA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="18">
        <location>
            <begin position="81"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="19">
        <location>
            <begin position="320"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR605437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="503" mass="57959" checksum="DBCD70EFA95FFD1A" modified="2020-06-17" version="1">MPICVCHSLLLLLLLLLPLLLLLPLLLLLLLLLLLLLPLLLFFCSSAFGPSKQEEEDYEDVPINKTWVLSPKVYESDVTLILNKLLQGYDNKLRPDIGVRPTVIETAVYINSIGPVDPINMEYTIDIFFAQTWYDSRLKFNSSMKLLMLNSNMVGKIWIPDTFFRNSRKSDAHWITTPNRLLRLWSNGRVMYTLRLTINAECYLKLHNFPMDEHSCPLEFSSCSARGSAPDGPSVQKDRSGLMWAVSGFSSDGYPKNEIMYRWQRRAVEVADQRYWRLYQFAFVGLRNSSDVAHTQSGEYVILTIFFDLSRRMGYFTIQTYIPCSMIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTISRKSLPKVSYVTAMDLFVSVCFIFTFAALMEYGTLHYFTSNRQTKKSKQRASSMVNIRPGTSLLQVNNIVPYHEEDDYAYECLDGKDCASFFCCFDDCRSGAWRENRMHVRVSKIDSYSRIFFPTAFGLFNLVYWIGYLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0N2</accession>
    <name>A0A673B0N2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">HAUS augmin-like complex, subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">haus1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030010285.1">
        <property type="nucleotide sequence ID" value="XM_030154425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B0N2"/>
    <dbReference type="FunCoup" id="A0A673B0N2">
        <property type="interactions" value="461"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036365.1">
        <property type="protein sequence ID" value="ENSSORP00005035415.1"/>
        <property type="gene ID" value="ENSSORG00005016726.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433154"/>
    <dbReference type="CTD" id="115106"/>
    <dbReference type="InParanoid" id="A0A673B0N2"/>
    <dbReference type="OrthoDB" id="5372507at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026243">
        <property type="entry name" value="HAUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25762">
        <property type="entry name" value="HAUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02087">
        <property type="entry name" value="HAUSAUGMINL1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="30679" checksum="C876988C708BA25D" modified="2020-06-17" version="1">MGEKIKKVKSWLSSVFGDQPVPEFEVNTRTVDILYQLAQSSEARCSDTALLIEDLKQKAAEYQAEGVHFQDVLLQGVGLSCASLSKASTDYLSALVDNAMVLGVRDTSLCSFLPAINNHTNVLLEAEKSNRRLERELMTLRKRLSTTLVLRSNLQEDINKTVKAQAVESAKAEERLLNMDFVTTKAKELSNRRERAEAQFASRNMDESLTHQAIVQLSEEVTALKQEIMPLNKKLEPYMDLSPSPSLAQVKIEEARRELAAIDSQLEMEMDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1V7</accession>
    <name>A0A673B1V7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B1V7"/>
    <dbReference type="Ensembl" id="ENSSORT00005035423.1">
        <property type="protein sequence ID" value="ENSSORP00005034503.1"/>
        <property type="gene ID" value="ENSSORG00005016283.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B1V7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="238" mass="27215" checksum="FCE5F1BC4D7CA01E" modified="2020-06-17" version="1">MSEGHWNHRLQTSEGHWNYRLQTNGGHWNHRLQTNGGHWNYRLQTNCRRVKDTGTTDCRLMKDTGTTDCRLMKDTGTTDYCRLMKDTGTTDCRQMEDTGTTDCRRMKDTGTTDYCRLMEDTGTTDCRLMKDTGTTDCRLMKDTGTTDYCRLMKDTGTTDCRLMEDTGTTDCRLMKDTGTTDCRLMEDTGTTDCRLMKDTGTTDCRLMEDTGTTDCRLMEDTGTTDCRLMKDTGTTDCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3Z8</accession>
    <name>A0A673B3Z8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L1 transposable element RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B3Z8"/>
    <dbReference type="Ensembl" id="ENSSORT00005037038.1">
        <property type="protein sequence ID" value="ENSSORP00005036079.1"/>
        <property type="gene ID" value="ENSSORG00005017032.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3Z8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.250.20">
        <property type="entry name" value="L1 transposable element, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042566">
        <property type="entry name" value="L1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33456" checksum="12EC9AC86605F209" modified="2020-06-17" version="1">EKQVKAHQKHQAVGHNEENMDRRVKFLTASQTLSCGLIDDGQVKQLHRPSQVLHVLRFILKMKILDFRFKYRWSKTQLLGLLQQAAMNENLTSIKTELQAIKSELSLSISNIKSDLGALKHTVGEMETSLSTCTDDIVSLQAKVDHLSAKLLKVVKKCEDLESRSRRNNIRIIGVPEDSPISATTTAISSMLKEALNLDKEPLLDRIHRTLQPKPKAAHQRIKTAGMTISIFPDYTAKTAWARAAFTDIRRQLRDIPGIWFGLLYAARLRVTYDGVEREFNSPEDARSFIKTLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B409</accession>
    <name>A0A673B409_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">slc6a2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B409"/>
    <dbReference type="FunCoup" id="A0A673B409">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037049.1">
        <property type="protein sequence ID" value="ENSSORP00005036089.1"/>
        <property type="gene ID" value="ENSSORG00005017041.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B409"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005330">
        <property type="term" value="F:dopamine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005334">
        <property type="term" value="F:norepinephrine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051583">
        <property type="term" value="P:dopamine uptake involved in synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF320">
        <property type="entry name" value="SODIUM-DEPENDENT NORADRENALINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0915">Sodium</keyword>
    <keyword evidence="7 15" id="KW-0769">Symport</keyword>
    <keyword evidence="4 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
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            <dbReference type="ChEBI" id="CHEBI:29101"/>
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            <dbReference type="ChEBI" id="CHEBI:29101"/>
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            <begin position="194"/>
            <end position="203"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036089.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="635" mass="71096" checksum="CC61968DACA048AC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B7U1</accession>
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        <recommendedName>
            <fullName evidence="10">Septin-type G domain-containing protein</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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            <begin position="651"/>
            <end position="923"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51719"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038386.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="952" mass="104379" checksum="438DE572FF4DEFE1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEE9</accession>
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        <recommendedName>
            <fullName evidence="6">Homeobox protein</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1 6 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the paired homeobox family. Bicoid subfamily.</text>
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        <property type="protein sequence ID" value="ENSSORP00005038982.1"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
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    <dbReference type="InterPro" id="IPR016233">
        <property type="entry name" value="Homeobox_Pitx/unc30"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882:SF2">
        <property type="entry name" value="PITUITARY HOMEOBOX 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000563">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="68"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="70"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="277" mass="30924" checksum="9C6DE0B6F4113C72" modified="2020-06-17" version="1">MISSLNCSAFFFFFTKMTVLNQNVHPSVLSASFVSVLTESVFIRLSDTEKSLQNQLDESSAEDGALKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVGTLPTRVRNQQAELCKNGFGAQFNGLMQPYDDMYTGYSYNNWATKSLASSPLSAKSFPFFNSMNVSPLSSQPMFSPPSSIPSMNMASAWCPRPTALNSVAVTAATCPYATSASPYMYRDTCNSSLASLRLKAKQHTNFAYPAVQNPVSNLSPCQYAVDRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFB8</accession>
    <name>A0A673BFB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">VWFC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">vwc2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030012669.1">
        <property type="nucleotide sequence ID" value="XM_030156809.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030012670.1">
        <property type="nucleotide sequence ID" value="XM_030156810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BFB8"/>
    <dbReference type="FunCoup" id="A0A673BFB8">
        <property type="interactions" value="660"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040310.1">
        <property type="protein sequence ID" value="ENSSORP00005039302.1"/>
        <property type="gene ID" value="ENSSORG00005018351.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434716"/>
    <dbReference type="CTD" id="375567"/>
    <dbReference type="InParanoid" id="A0A673BFB8"/>
    <dbReference type="OrthoDB" id="8574072at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042979">
        <property type="entry name" value="VWC2/VWC2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057856">
        <property type="entry name" value="VWC2L_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059152">
        <property type="entry name" value="VWC2L_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46252:SF4">
        <property type="entry name" value="BRORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46252">
        <property type="entry name" value="BRORIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23333">
        <property type="entry name" value="VWC2L_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23334">
        <property type="entry name" value="VWC2L_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23331">
        <property type="entry name" value="VWC2L_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025613753" description="VWFC domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="9">
        <location>
            <begin position="202"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="311" mass="34305" checksum="EF6759296509E8AE" modified="2020-06-17" version="1">MLRSVAMTAEVLFVLGFLMSAAQSNPIATLPASRERLEKVLTQAREDKHQDKQAPEQTAGFGGQQQLEKPEINPVSSNRTAVSRTRANPSSKTQGSNGGKSQEQWEQETLNQIDEGPTSDVTLSMDPIDEYAYPDYRGKGCMDESGFVFAIGEQFTPGPSTCPCLCTDEGPLCSKPECPKVHPRCIKVDTSQCCPQCKEKKNYCEFRGKIYASLEEFKVSPCEKCRCEPSGEVLCSVAACPQTECVDPEYEPDQCCPICKSGPNCYADTAVIPAGREVKIDECTICYCTYEEGTWQIERQATCSKNECQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKY9</accession>
    <name>A0A673BKY9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">SRY-box transcription factor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">sox5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BKY9"/>
    <dbReference type="Ensembl" id="ENSSORT00005042339.1">
        <property type="protein sequence ID" value="ENSSORP00005041277.1"/>
        <property type="gene ID" value="ENSSORG00005019124.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BKY9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032332">
        <property type="term" value="P:positive regulation of chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22042">
        <property type="entry name" value="HMG-box_EGL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000003">
        <property type="entry name" value="Putative transcription factor SOX-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051356">
        <property type="entry name" value="SOX/SOX-like_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789:SF3">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="11">
        <location>
            <begin position="553"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="553"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="523"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="683"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="197"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="452"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="683"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="715"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="731"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50118"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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    <accession>A0A673BUY0</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="6">Decreases the sensitivity of PDP1 to magnesium ions, and this inhibition is reversed by the polyamine spermine.</text>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046676.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="892" mass="99917" checksum="EBAAB180D2ED03FF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Peripheral membrane protein</topology>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVT1</accession>
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            <taxon>Vertebrata</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Gobiaria</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="8">May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1.</text>
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    <feature type="region of interest" description="Disordered" evidence="10">
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <begin position="55"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="186" mass="20561" checksum="14EC45882C662982" modified="2020-06-17" version="1">MGDKRSPTRPKRQPKPSSDEGFWDCSVCTYKNTAEAFKCMMCDVRKGTSTRKPRPVSQLVSQQQVTQQFVLPSQPKKEKKERVEREKGDREPALKKNSHKKMRPRLKNIDRSSAQHLEVTVGDLTVIITDFKEKAKPSSTSATSSSAASSADHHSQNGSSSENTEKGLSRSSSPRGEGSSVNGESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BW83</accession>
    <name>A0A673BW83_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nudcd2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030000696.1">
        <property type="nucleotide sequence ID" value="XM_030144836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BW83"/>
    <dbReference type="FunCoup" id="A0A673BW83">
        <property type="interactions" value="938"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046539.1">
        <property type="protein sequence ID" value="ENSSORP00005045389.1"/>
        <property type="gene ID" value="ENSSORG00005020849.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426694"/>
    <dbReference type="CTD" id="134492"/>
    <dbReference type="InParanoid" id="A0A673BW83"/>
    <dbReference type="OrthoDB" id="515366at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000105">
        <property type="entry name" value="NudC domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.740:FF:000001">
        <property type="entry name" value="nudC domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.740">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037898">
        <property type="entry name" value="NudC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356">
        <property type="entry name" value="NUCLEAR MOVEMENT PROTEIN NUDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356:SF18">
        <property type="entry name" value="NUDC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="1">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="157" mass="17615" checksum="D0EF1F4DE7241E02" modified="2020-06-17" version="1">MSVHFEERSGVVPCKTPWGSWYQTMEEVFVEVEVPHGTSAKEVKCQLGSKNIELHVKGKEIFKGKLFGTTVSDEATWTLEDKCLIQIVLMKANREAGNCWSSLLEGEYCANAWVQDQMQRKLTLERFQRENPGFDFSGAEISGNFAGGGPDFSSLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXZ4</accession>
    <name>A0A673BXZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Meteorin, glial cell differentiation regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">metrn</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the meteorin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029978161.1">
        <property type="nucleotide sequence ID" value="XM_030122301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BXZ4"/>
    <dbReference type="FunCoup" id="A0A673BXZ4">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046668.1">
        <property type="protein sequence ID" value="ENSSORP00005045517.1"/>
        <property type="gene ID" value="ENSSORG00005020912.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410592"/>
    <dbReference type="CTD" id="79006"/>
    <dbReference type="InParanoid" id="A0A673BXZ4"/>
    <dbReference type="OrthoDB" id="6092325at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051998">
        <property type="entry name" value="Meteorin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28593:SF2">
        <property type="entry name" value="METEORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28593">
        <property type="entry name" value="METEORIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="33537" checksum="31558F61AEF6BC58" modified="2020-06-17" version="1">MSGGGVWIYAFWILLVAGVDLVQSYYSEDQCSWRGSGLSQQQGSVEQISLHCSEGTLDWLYPKGALRLTLSPRLPSVAVGPGGSSSGLITACVKPSEQFHGAQLYLERDGVLELLVGDRLESSPPPRVRCFSRLPGERVALFMQATPHQDISRRIASFRYELRGDWTARLSLDSNPISNEDACRPCNNTEILMAVCTSDFVVRGNIRSVEEDENLRAAVIKVSATRVFRQKYALFTGNSRLTSQGEIRTLLQCGVKPGPGSFLFTGRVHFGEAWLGCAPRYKDFQQAYIIAKAAQQIPCELPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BY96</accession>
    <name>A0A673BY96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="23">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">ppp5c</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="23"/>
    <dbReference type="AlphaFoldDB" id="A0A673BY96"/>
    <dbReference type="FunCoup" id="A0A673BY96">
        <property type="interactions" value="2312"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047169.1">
        <property type="protein sequence ID" value="ENSSORP00005046014.1"/>
        <property type="gene ID" value="ENSSORG00005020726.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BY96"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07417">
        <property type="entry name" value="MPP_PP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000055">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000017">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041753">
        <property type="entry name" value="PP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013235">
        <property type="entry name" value="PPP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08321">
        <property type="entry name" value="PPP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 22" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="22">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="24">
        <location>
            <begin position="280"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="21">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="479" mass="54838" checksum="B0B7E5D7C6C20D1F" modified="2020-06-17" version="1">MAEGGNDAELLKEKANKYFKEKDYENAIKYYTEALELNPSNAIYYSNRSLAYLRTECYGYALADATKALEIDKNYIKGYYRRATSNMALGKFKAALKDYETVVRVRPNDKDATMKYQECSKIVKQKAFERAIASDETKKSVVDSLDIENMTIEDDYVGPKLEDGKVTLTFMKEMMDWFQDQKKLHRKCAYQILVQVKDVLSKLPSLVEITLKESEKITICGDTHGQYYDLLNIFKLNGLPSETNPYLFNGDFVDRGSFSLEVILTLFGFKLLFPDNFHLLRGNHETDNMNQMYGFEGEVKAKYTAQMFQLFSEVFQWLPLAQCINNKVLIMHGGLFSEDGVTLDDLRKIDRNRQPPDSGPMCDLLWSDPQPQNGRSISKRGVSCQFGPDVTERFLEQNKLDFIVRSHEVKAEGYEVTHSGKCITVFSAPNYCDQMGNKGAYIHLRGSDLKPEFHQFTAVPHPNVKPMAYANTLMQLGMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZ23</accession>
    <name>A0A673BZ23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">rnf167</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 25">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673BZ23"/>
    <dbReference type="Ensembl" id="ENSSORT00005047428.1">
        <property type="protein sequence ID" value="ENSSORP00005046269.1"/>
        <property type="gene ID" value="ENSSORG00005021192.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BZ23"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02123">
        <property type="entry name" value="PA_C_RZF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16796">
        <property type="entry name" value="RING-H2_RNF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000099">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051653">
        <property type="entry name" value="E3_ligase_sorting_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044744">
        <property type="entry name" value="ZNRF4/RNF13/RNF167_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47168:SF1">
        <property type="entry name" value="OS02G0798600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47168">
        <property type="entry name" value="RING ZINC FINGER DOMAIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025550403" description="RING-type E3 ubiquitin transferase" evidence="21">
        <location>
            <begin position="26"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="235"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="283"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="353"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="399" mass="44400" checksum="E7F3403225E48A1C" modified="2020-06-17" version="1">MMPFSVWSVAILMFCSILVSSLTHAFIYGHFGNRTSPLFEDLPALFGSPLPNEGLMGILVVTRPLNACAPIDPAPPLPSSFDANTTKVIALVRRYDCNFDIKVLHAQQAGFDAAIVHNMYSDNLLNMNYSNETIAEEIQIPSVFVSYYASQFLKTYVIPEQGSYVIIKPESAFPFTYYLIPFCGVVGMIILVMCVILIIRCVQYRKRLRKNRLSKEQLKRIPTHRFTKGDDYDVCAICLDEYEEGDKLRVLPCSHAYHCKCVDPWLTQTKKTCPVCKQRVTRPSAEHSESESEEESGGRRSVEDGTEGEADSERTPLLRPSNPGSPSGSPGAYSATNNTTVTTAQCLASPARCDSPIVGYEGYYSPEEDTDSDSDDTGEERHHTDDDTAQLIGRDRVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZP3</accession>
    <name>A0A673BZP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deaminated glutathione amidase</fullName>
            <ecNumber evidence="3">3.5.1.128</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nitrilase homolog 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nit1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.128" evidence="3"/>
    <dbReference type="RefSeq" id="XP_030017447.1">
        <property type="nucleotide sequence ID" value="XM_030161587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BZP3"/>
    <dbReference type="Ensembl" id="ENSSORT00005047310.1">
        <property type="protein sequence ID" value="ENSSORP00005046152.1"/>
        <property type="gene ID" value="ENSSORG00005021149.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438166"/>
    <dbReference type="CTD" id="4817"/>
    <dbReference type="InParanoid" id="A0A673BZP3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110050">
        <property type="term" value="F:deaminated glutathione amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000005">
        <property type="entry name" value="nitrilase homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="7">
        <location>
            <begin position="36"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00066912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="318" mass="35184" checksum="FCEAA7BA5116425C" modified="2020-06-17" version="1">MLTFRCILKPSVQYLSCSSALTQRIHLQNRMSTSPHPLAAVCQVTATPDVEANFSACKQLVEEARGRGAGMVFLPEGFDYIGSSREETLSLSQDLEGDTVSRYKELARRLDVWLSLGGFHERGQNWESDRRIYNSHIIINNTGDIVSVYRKAHLFDVELTDKGVSLKESAFTIPGPSLVPPVQTPIGKVGLGICYDLRFPELSIALQRQGADILTYPSAFTVATGAAHWEVLLRARAIETQCYVLAAAQVGRHHDKRSSYGHAVAVDPWGEVVGDCGGENPGLVMVEINLDKVSSTRRNMPVQQHRRDTGFYHSLEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0Y3</accession>
    <name>A0A673C0Y3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Niban-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">niban2a</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Niban family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C0Y3"/>
    <dbReference type="FunCoup" id="A0A673C0Y3">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050088.1">
        <property type="protein sequence ID" value="ENSSORP00005048896.1"/>
        <property type="gene ID" value="ENSSORG00005021505.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C0Y3"/>
    <dbReference type="OrthoDB" id="9010513at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd23949">
        <property type="entry name" value="Niban-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026088">
        <property type="entry name" value="Niban-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059060">
        <property type="entry name" value="Niban_1/2/3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14392">
        <property type="entry name" value="NIBAN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14392:SF2">
        <property type="entry name" value="PROTEIN NIBAN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26086">
        <property type="entry name" value="Niban2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26089">
        <property type="entry name" value="PH_Niban2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="68"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="638"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="697"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="778"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="660"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="699"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="852" mass="95988" checksum="4F1A90B748961584" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1H4</accession>
    <name>A0A673C1H4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C1H4"/>
    <dbReference type="Ensembl" id="ENSSORT00005047382.1">
        <property type="protein sequence ID" value="ENSSORP00005046223.1"/>
        <property type="gene ID" value="ENSSORG00005021178.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1H4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16949" checksum="86910118E5247385" modified="2020-06-17" version="1">MPRNYTRKTSWGQTPLAEMESAAAEVMQGKKSLRKAGRDRNIDKTTLQRFIKKKEKGEVKSVAWGAVAEAKRIFTDAMEEELAKHLKQLADQFHGLAPVKCRGLAFEYAERNNIPVPTNWTEKQCAGRLGCARDDMILETRKSGRDPSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7D2</accession>
    <name>A0A673C7D2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Potassium voltage-gated channel, KQT-like subfamily, member 5a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">kcnq5a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C7D2"/>
    <dbReference type="Ensembl" id="ENSSORT00005050342.1">
        <property type="protein sequence ID" value="ENSSORP00005049144.1"/>
        <property type="gene ID" value="ENSSORG00005022300.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C7D2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000016">
        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF8">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="108"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="17">
        <location>
            <begin position="435"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="398"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="640"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="790"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="858"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="867"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="953" mass="105651" checksum="FCF0378D214971A7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CC38</accession>
    <name>A0A673CC38_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Crumbs cell polarity complex component 2b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CC38"/>
    <dbReference type="Ensembl" id="ENSSORT00005052878.1">
        <property type="protein sequence ID" value="ENSSORP00005051645.1"/>
        <property type="gene ID" value="ENSSORG00005023317.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CC38"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048592">
        <property type="term" value="P:eye morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051093">
        <property type="term" value="P:negative regulation of developmental process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000565">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="100"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="138"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="175"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="24653" checksum="B7101B211344E2C1" modified="2020-06-17" version="1">MSAPWAQSSRWTLVLDDETEETSISRSQGGNLDFLRQGVTIFLGGLGPDAGWSLAGCLSTVELGGIALPYFSSSDVNLPRLQEERFVQTSLNSALLGCSGASVCEPNPCMNGGDCQDLFNLYNCTCSEGWAGRRCDFYLDTCASGPCVHGNCSVHGLSYECSCEFGYAGVNCEEEVDVCENHLCANGGTCLHGPDRYACLCPDNYTGPLCRYVQGVGTRRRLKTTSDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCA4</accession>
    <name>A0A673CCA4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LNR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CCA4"/>
    <dbReference type="Ensembl" id="ENSSORT00005052943.1">
        <property type="protein sequence ID" value="ENSSORP00005051710.1"/>
        <property type="gene ID" value="ENSSORG00005023340.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCA4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043543">
        <property type="entry name" value="PAPPA/PAPPA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46130">
        <property type="entry name" value="LAMGL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46130:SF1">
        <property type="entry name" value="PAPPALYSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00004">
        <property type="entry name" value="NL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="LNR" evidence="5">
        <location>
            <begin position="265"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="350" mass="38827" checksum="72A7A54BA1B8B96E" modified="2020-06-17" version="1">MSFPVPPSCLLRVFFDSYFQRETKLECFHGSWDRIVNQSHVYHAIFSCPWGTTFGKQCTFTCGSPAILQGDSDRLVCLEDGLWSFPEAYCKIECPEASGVNNAKLLTADCLATGHDVGSVCRYKLHVNKWCKIQFLLARAWEDGGCEPISCPAIADIFQGMYTCTNGRFYDSTCTLRRWTASFSMCSRLQGSCSPPPDLHSVEYSCDQGTAVGKCRCFPTCIVSLDMDLRDPVVLPNGTTAESLNHWILPPKSSIICTGLMKWYPDPRHIHCIQSCEPFGGDGWCDTINNRAYCQYDGGDCCPSTLSSRKVIQFGADCNQDECTCRDPDAEENKSKAKRLEGGGARGGMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CD16</accession>
    <name>A0A673CD16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Probable ATP-dependent RNA helicase DDX23</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DEAD box protein 23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ddx23</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation. Independently of its spliceosome formation function, required for the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">The phosphorylated form (by SRPK2) is a component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Identified in the spliceosome C complex. Interacts with ERBB4. Interacts with ERCC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_029994712.1">
        <property type="nucleotide sequence ID" value="XM_030138852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CD16"/>
    <dbReference type="FunCoup" id="A0A673CD16">
        <property type="interactions" value="1624"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051211.1">
        <property type="protein sequence ID" value="ENSSORP00005050008.1"/>
        <property type="gene ID" value="ENSSORG00005022534.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422503"/>
    <dbReference type="CTD" id="9416"/>
    <dbReference type="InParanoid" id="A0A673CD16"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17945">
        <property type="entry name" value="DEADc_DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000322">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000520">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057479">
        <property type="entry name" value="PRP28/DDX23-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25430">
        <property type="entry name" value="DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="377"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="408"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="637"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="377"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="133"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="806" mass="94163" checksum="8351C95053024F9A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CD42</accession>
    <name>A0A673CD42_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 178B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tmem276a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM178 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CD42"/>
    <dbReference type="Ensembl" id="ENSSORT00005051882.1">
        <property type="protein sequence ID" value="ENSSORP00005050667.1"/>
        <property type="gene ID" value="ENSSORG00005022959.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CD42"/>
    <dbReference type="OrthoDB" id="8809386at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039625">
        <property type="entry name" value="T178A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005:SF3">
        <property type="entry name" value="SI:CH211-150G13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="300" mass="33787" checksum="3E95FEB2921C5897" modified="2020-06-17" version="1">MAAMRTLTVAGLFLAFCALGLIAIAISTDNWYETDARRHRERCKNYSNKRNDPGYIYISNNNLPLRMLPKEKNVERKGSSVSGEMLLRAKRHFLPPAPAMESLCSRQFNSTITGLWRKCHRQGFDLEMEDSIFKGLVPRCTPIKYYYSSSVLPRNLPINLTKTIRQDEWHALHLQRMTASFVGMAISIILFGWIIGVLGCCKEHDLMQYVAGLLFLMGGTCCIISLCTCVAGINFELSRYPRYMFGIPEDISHGYGWSMFCAWGGLGLTLLAGFLCTLAPSLYPPHTPVAHKPRQENGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CDF7</accession>
    <name>A0A673CDF7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NEK6-subfamily protein kinase</fullName>
            <ecNumber evidence="14">2.7.11.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">nek7</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.34" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A673CDF7"/>
    <dbReference type="FunCoup" id="A0A673CDF7">
        <property type="interactions" value="406"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053363.1">
        <property type="protein sequence ID" value="ENSSORP00005052120.1"/>
        <property type="gene ID" value="ENSSORG00005023330.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CDF7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08224">
        <property type="entry name" value="STKc_Nek6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000148">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000204">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000240">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="47"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="315" mass="36255" checksum="575B501019950F6F" modified="2020-06-17" version="1">QSNQQQPRASSQQNKLQKPGLNTECSLFLQHSSLPSSYMGHNSLANFQIEKKIGRGQFSEVYRARYLLDNTSVALKKVQIFDLMDAKARQDCIKEIDLLKQLNHPNVIKYHASFIEDNELNIVLELADAGDLSRMIKHFKKQRRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRNLVDMCINPDPEKRPDITYVYDIAKHMQSLTRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLG5</accession>
    <name>A0A673CLG5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673CLG5"/>
    <dbReference type="Ensembl" id="ENSSORT00005055421.1">
        <property type="protein sequence ID" value="ENSSORP00005054149.1"/>
        <property type="gene ID" value="ENSSORG00005024274.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLG5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000003">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000006">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025462313" description="non-specific serine/threonine protein kinase" evidence="12">
        <location>
            <begin position="25"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="35"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="14">
        <location>
            <begin position="631"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="321"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="435"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="331"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="944" mass="107541" checksum="7B9DE9CE0BFB238B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLL5</accession>
    <name>A0A673CLL5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycolipid transfer protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gltpa</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Accelerates the intermembrane transfer of various glycolipids. Catalyzes the transfer of various glycosphingolipids between membranes but does not catalyze the transfer of phospholipids. May be involved in the intracellular translocation of glucosylceramides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLTP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CLL5"/>
    <dbReference type="FunCoup" id="A0A673CLL5">
        <property type="interactions" value="1153"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057823.1">
        <property type="protein sequence ID" value="ENSSORP00005056526.1"/>
        <property type="gene ID" value="ENSSORG00005025140.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLL5"/>
    <dbReference type="OrthoDB" id="205255at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000003">
        <property type="entry name" value="glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF97">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="209" mass="23923" checksum="1A068D2E80060BFD" modified="2020-06-17" version="1">MALLMEHQFRQLPADRQVETRPFLEAVSYLPPFFDCLGSTVFAPIKADISGNITKIKAVYDTNPGRFKTLGQILEAEKEMHGAEWPKVGATLALMWLKRGLRFIQVFLQSLVDGEKDDNNPNHIRVNANKAYEIALKRYHGWLVQQLFKAALFAAPYKSDFLRALSKGRDVKEEECLDKIRKFLINFSATVDAIYEMYSKLNADLDYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLU5</accession>
    <name>A0A673CLU5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Short-chain dehydrogenase/reductase 3</fullName>
            <ecNumber evidence="3">1.1.1.300</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">dhrs3b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>all-trans-retinol + NADP(+) = all-trans-retinal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25033"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.300"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.300" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673CLU5"/>
    <dbReference type="Ensembl" id="ENSSORT00005054858.1">
        <property type="protein sequence ID" value="ENSSORP00005053602.1"/>
        <property type="gene ID" value="ENSSORG00005024098.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLU5"/>
    <dbReference type="OrthoDB" id="6251714at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004745">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048385">
        <property type="term" value="P:regulation of retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF483">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ketoreductase" evidence="15">
        <location>
            <begin position="39"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="302" mass="33847" checksum="1FAE143B18F3CEC0" modified="2020-06-17" version="1">MDVKSACRMFLFPVQMLYHIIRATLFSLLPSRRKDLTKEVVLITGGGRGIGRHLAKEFVKQGAKKVILWGRTEKCLKETAEEICLSGTECHYFICDVANREEVYKQAKVVREKVGDVTILVNNAAVVHGKSLMDSDDDALLKSQHINTMGQFWTTKAFLPRMLELQHGHVVCINSILSQSPIPGAIDYCTSKASSLAFMESLTLGLLDCPGVGCTTVLPFHTNTEMFQGMRVRFPQLFPPLKPEVVAQKTVDAVRSDKAFLYLPWTMHALVILKSFMPQIALEEIHKFSGSYTCMNTFKGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CPF3</accession>
    <name>A0A673CPF3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gamma-glutamyltransferase YwrD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115417395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CPF3"/>
    <dbReference type="Ensembl" id="ENSSORT00005056503.1">
        <property type="protein sequence ID" value="ENSSORP00005055224.1"/>
        <property type="gene ID" value="ENSSORG00005024670.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CPF3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052896">
        <property type="entry name" value="GGT-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881:SF1">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="62165" checksum="057356766BA1DA7E" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSE3</accession>
    <name>A0A673CSE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CSE3"/>
    <dbReference type="Ensembl" id="ENSSORT00005059902.1">
        <property type="protein sequence ID" value="ENSSORP00005058576.1"/>
        <property type="gene ID" value="ENSSORG00005025845.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSE3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000013">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF15">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025332458" description="SRCR domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="43"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="89"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="191"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="35407" checksum="A91CE3F006706402" modified="2020-06-17" version="1">MLHVFLLVPLLPVTCSDLKKNKNKHPGAISPQHTEAHPQFQQYLLINQDLNHHRSSRCEGTLEVKQHEEWHEVVGIVWTLKAAAVLYSVRLVQGSSLCSGRLEVRSNQSWSSVCEDDLDHNDTEVICRELGCGAPSPLQGALYGEGEAPVWTSEFQCDGNESALLDCRRSNSTGKTCSTGKAAELTCRRGVRLVGGASRCAGNLEVKLQDEIWRPVDGFYWNLRSANRTCADLDCGSAVSIRIRDEGSDRDVWRIRPECDESSLKNCFRSQTMKSSSSLELNCSGRISNNVSTDQHRKTKLTDIQCGDTWMQLRINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CUK6</accession>
    <name>A0A673CUK6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">EF-hand domain (C-terminal) containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">efhc1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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</entry>
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            <taxon>Apogoninae</taxon>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="421" mass="48531" checksum="437781C1F8403324" modified="2020-06-17" version="1">WKIPLQNFLNWCNTVGLVLSDKVYVSRDGTVLFTIPRSALLHQGTTQISALLEREKRTLQSSSGWVPLLLTLLYEYTSTQSHWKPYLSLWTDFRKLDHPMFWSKEERDRLLRGTGIPEAVDRDLTNIQREYTDVVLPFITKHPDLWNPQVHTLERYTQLVAFVMAYRSGRRSCVFKGGGGDPEPPMMVPMADMLNHISNHNANLEFTPDSLKMVCVRPIHKGEEVFNTYGQMANWQLLHMYGFTEPYPSNSNDTADIPPPTSTKLYRVSRLSVECGFHWPQVEEKVEMLGQIMQDKGVFVFGKQGCLTDTDLHTALKVLSMSQDEFSEFKENEGWEEEEDDEDARICQAFSNEGLPELKAPWKRLLHEAARLTLRSYTDGDRRLMEDGAAVAKLSTRQRNALQVRHGQKDILYRLMELTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y537</accession>
    <name>A0A672Y537_9TELE</name>
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        </submittedName>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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    </organism>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NF-KAPPA-B INHIBITOR ZETA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="247"/>
        </location>
    </feature>
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        <location>
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            <end position="294"/>
        </location>
    </feature>
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            <end position="363"/>
        </location>
    </feature>
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        <location>
            <begin position="365"/>
            <end position="397"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="509"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="516" mass="55815" checksum="C60A51798BAB9AE1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y707</accession>
    <name>A0A672Y707_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="6">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">zgc:174895</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y707"/>
    <dbReference type="Ensembl" id="ENSSORT00005002245.1">
        <property type="protein sequence ID" value="ENSSORP00005002188.1"/>
        <property type="gene ID" value="ENSSORG00005001329.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y707"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000069">
        <property type="entry name" value="Zgc:174895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050120">
        <property type="entry name" value="Adenine_PRTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776:SF7">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="53"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="200" mass="21768" checksum="4A19050A880DE83A" modified="2020-06-17" version="1">MDVLAVPADGHKGWYLSLMAPNTKGPTFAWLDPSRLYCNSQALADCVKDLLNPFQNDSIDLVAGIDAMGFILGASVAATLGKGFLAIRKAGHLCVATQNQNYTDYTGKEKTLEVRVDVLKPGVRVLIVDQWIETGGTMKAAVQLVERLGATVVGVAAVAIENTEGGKWIKDNYKFSHCIPEDLQSQIDQKYLESFKSFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8K2</accession>
    <name>A0A672Y8K2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y8K2"/>
    <dbReference type="Ensembl" id="ENSSORT00005000098.1">
        <property type="protein sequence ID" value="ENSSORP00005000084.1"/>
        <property type="gene ID" value="ENSSORG00005000073.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8K2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052310">
        <property type="entry name" value="Kelch/BTB_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45972">
        <property type="entry name" value="BTB_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45972:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="69" mass="7891" checksum="33C50C2C821E60F8" modified="2020-06-17" version="1">MNEARPRCKLAALEGYIYAIGGECLSSVERYDPRLDRWTFVAPLPNDTFAVAHHVTLIYNVFNDCIFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9H4</accession>
    <name>A0A672Y9H4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 22">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="4 22">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC115422357</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="function">
        <text evidence="18">Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(R)-malate + a quinone = enol-oxaloacetate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:79827"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:17479"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:79828"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(S)-malate + a quinone = enol-oxaloacetate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:79831"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:17479"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:79832"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="22">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="22">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="22">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 22">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Mitochondrion inner membrane</location>
            <topology evidence="1 22">Peripheral membrane protein</topology>
            <orientation evidence="1 22">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4 22"/>
    <dbReference type="RefSeq" id="XP_029994503.1">
        <property type="nucleotide sequence ID" value="XM_030138643.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029994504.1">
        <property type="nucleotide sequence ID" value="XM_030138644.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029994505.1">
        <property type="nucleotide sequence ID" value="XM_030138645.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029994506.1">
        <property type="nucleotide sequence ID" value="XM_030138646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y9H4"/>
    <dbReference type="Ensembl" id="ENSSORT00005000379.1">
        <property type="protein sequence ID" value="ENSSORP00005000356.1"/>
        <property type="gene ID" value="ENSSORG00005000182.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000462.1">
        <property type="protein sequence ID" value="ENSSORP00005000437.1"/>
        <property type="gene ID" value="ENSSORG00005000182.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000480.1">
        <property type="protein sequence ID" value="ENSSORP00005000455.1"/>
        <property type="gene ID" value="ENSSORG00005000182.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000482.1">
        <property type="protein sequence ID" value="ENSSORP00005000457.1"/>
        <property type="gene ID" value="ENSSORG00005000182.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000494.1">
        <property type="protein sequence ID" value="ENSSORP00005000469.1"/>
        <property type="gene ID" value="ENSSORG00005000182.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422357"/>
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        <property type="reaction ID" value="UER01006"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:electron transfer activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:tricarboxylic acid cycle"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
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    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00197">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 22" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="17 22" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="6 22" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 22" id="KW-0408">Iron</keyword>
    <keyword evidence="14 22" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 22" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 22" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="23">
        <location>
            <begin position="40"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="24">
        <location>
            <begin position="176"/>
            <end position="206"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004443"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00047404"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048512"/>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="31868" checksum="DF38AB9535FECF47" modified="2020-06-17" version="1">MSVSWGPLSRTVCAVHNCGAMVMVRYAQTAAAPAPAPRIKKFQIYRWDPDTPGDKPRMQTYEVDLNVCGPMVLDALIKIKNEMDPTLTFRRSCREGICGSCAMNINGGNTLACLNKIDTNTSKPTKIYPLPHMYVVKDLVPDMSNFYAQYKSIEPYLKKKDESLEGKDQYYQSVEDRQKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDEFTEERLSKLQDPFSLYRCHTIMNCTKTCPKGLNPGKAIAEIKKMMATYKEKKTAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YB31</accession>
    <name>A0A672YB31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YB31"/>
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        <property type="gene ID" value="ENSSORG00005001078.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="81"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="175" mass="19698" checksum="2C32CA2AF224CC16" modified="2020-06-17" version="1">ITSVLLFFVEKTHQFPRNHSGPQHNHGPNRQGGSGDQKKPGGTNTNGQQPEPSETTSLNEALTLDLQSFRKPGEKTFTQRSRLFVGNLPNGVTEEDLEKLFSKYGKANEVFINKERGFGFIRLETRIIAEIARAELDDTPFRGRPIRFSVTLRSMTPACLHSDSNYSIHEQSIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDA3</accession>
    <name>A0A672YDA3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger, RAN-binding domain containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">zranb3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YDA3"/>
    <dbReference type="FunCoup" id="A0A672YDA3">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000919.1">
        <property type="protein sequence ID" value="ENSSORP00005000891.1"/>
        <property type="gene ID" value="ENSSORG00005000519.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18010">
        <property type="entry name" value="DEXHc_HARP_SMARCAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000024">
        <property type="entry name" value="DNA annealing helicase and endonuclease ZRANB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000788">
        <property type="entry name" value="DNA annealing helicase and endonuclease ZRANB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF3">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="48"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="318"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="522"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="866"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="868"/>
            <end position="881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1060" mass="119324" checksum="4052137C640539DA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDP4</accession>
    <name>A0A672YDP4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ppiaa</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672YDP4"/>
    <dbReference type="Ensembl" id="ENSSORT00005003433.1">
        <property type="protein sequence ID" value="ENSSORP00005003334.1"/>
        <property type="gene ID" value="ENSSORG00005002035.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDP4"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000013">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="7"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="164" mass="17501" checksum="5F69071BC7C7983E" modified="2020-06-17" version="1">MANPRVYFDVTADGAPLGRVVMELRPDVVPKTAENFRALCTGEKGFGYKGCSFHRIIPNFMCQGGDFTNHNGTGGKSIYGNKFADENFTLKHTGAGILSMANAGPNTNGSQFFICTAQTAWLDGKHVVFGSVVEGLDVVKKMESKGSNSGKPTCKIVIADCGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFG1</accession>
    <name>A0A672YFG1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ectonucleotide pyrophosphatase/phosphodiesterase 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YFG1"/>
    <dbReference type="Ensembl" id="ENSSORT00005002761.1">
        <property type="protein sequence ID" value="ENSSORP00005002681.1"/>
        <property type="gene ID" value="ENSSORG00005001646.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFG1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF63">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025522423" evidence="2">
        <location>
            <begin position="18"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="423" mass="48304" checksum="456FA73C43E74713" modified="2020-06-17" version="1">MLFLAFFILIGCPPCAAAPAHHSTSEIPLASRSSSRNKLLLISFDGFRWDYDRLVETPNLDRMAQDGEGHLRYATVHHHHQPLTLYDNHGVIHNIWFNTTTQEKKQYYMTQFVDSYWDNGSLPIWITAQRQGLKTGSLHFPGTAATYRGETVQVRQVEPMFYDHSNEAEWRTNVQKVIGDWFHQQDLDFVSLYFGEPDLAGHKHGPDSPEVKEMIRQVDRTVGYIRDTVRDHGLSDQLNIIMTTDHGMTATLRGGLVEEILLSKIPGFSFRDIQFQLLDYGPVGMLLPKEGQLEKVYKALKGEVPARLHYSTHPRLLPLILIADPGYIIWLLHNNKGDHGFDNEIMDMKAFFRAVGTPPPPPSAFSFKTPPDIALYLCLFVYCLFVCFDDVYVIMCIFFIYSEGVVGCFMSCSVTCLWTVDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFH7</accession>
    <name>A0A672YFH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylglucosamine transferase subunit ALG14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparagine-linked glycosylation 14 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UDP-N-acetylglucosamine transferase subunit alg14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115410887</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Forms with ALG13 the active heterodimeric UDP-N-acetylglucosamine transferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ALG14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029978571.1">
        <property type="nucleotide sequence ID" value="XM_030122711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YFH7"/>
    <dbReference type="Ensembl" id="ENSSORT00005002844.1">
        <property type="protein sequence ID" value="ENSSORP00005002761.1"/>
        <property type="gene ID" value="ENSSORG00005001712.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410887"/>
    <dbReference type="InParanoid" id="A0A672YFH7"/>
    <dbReference type="OrthoDB" id="17098at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000098">
        <property type="entry name" value="UDP-N-acetylglucosamine transferase subunit ALG14 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013969">
        <property type="entry name" value="Oligosacch_biosynth_Alg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154:SF4">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08660">
        <property type="entry name" value="Alg14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="216" mass="24042" checksum="2CEB1F554AA2F948" modified="2020-06-17" version="1">MSFLPVVVFVLIFLCFVFLLRLYVVIKTGSKYKPGSKGSVVVLAVAGSGGHTTELLRLMDSLSPSYKPRHYVIADTDKISEEKICTFESSKELRASEPQFTICRVPRSREVHQSWSSSVISTLHALLYSVPLVYKLRPDLVLCNGPGTCVPLCAAGLLLGILGMKKVLIVYVESICRVDSLSVTGRILYPVSDYFFVQWATLRDKYPKAIYLGRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGR1</accession>
    <name>A0A672YGR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">aromatase</fullName>
            <ecNumber evidence="11">1.14.14.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cytochrome P-450AROM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Estrogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115413076</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of aromatic C18 estrogens from C19 androgens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>androst-4-ene-3,17-dione + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = estrone + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:38195"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>testosterone + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 17beta-estradiol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:38191"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.14" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A672YGR1"/>
    <dbReference type="FunCoup" id="A0A672YGR1">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003849.1">
        <property type="protein sequence ID" value="ENSSORP00005003740.1"/>
        <property type="gene ID" value="ENSSORG00005001811.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YGR1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070330">
        <property type="term" value="F:aromatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008585">
        <property type="term" value="P:female gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20616">
        <property type="entry name" value="CYP19A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000032">
        <property type="entry name" value="Cytochrome P450 aromatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF204">
        <property type="entry name" value="AROMATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 16 17" id="KW-0349">Heme</keyword>
    <keyword evidence="7 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="548" mass="62043" checksum="E50449BBBA03BF9A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ73</accession>
    <name>A0A672YJ73_9TELE</name>
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        <submittedName>
            <fullName evidence="16">High affinity choline transporter 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115428191</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030147067.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030003008.1">
        <property type="nucleotide sequence ID" value="XM_030147148.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005004604.1"/>
        <property type="gene ID" value="ENSSORG00005002802.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428191"/>
    <dbReference type="InParanoid" id="A0A672YJ73"/>
    <dbReference type="OrthoDB" id="546820at2759"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005307">
        <property type="term" value="F:choline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:acetylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11474">
        <property type="entry name" value="SLC5sbd_CHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052244">
        <property type="entry name" value="Choline_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45897:SF5">
        <property type="entry name" value="HIGH AFFINITY CHOLINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45897">
        <property type="entry name" value="HIGH-AFFINITY CHOLINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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            <end position="143"/>
        </location>
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        </location>
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        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="552" mass="60171" checksum="116F474A18827D61" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJA8</accession>
    <name>A0A672YJA8_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Leucine rich repeat containing 74A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20007" checksum="FADA6A6582400785" modified="2020-06-17" version="1">FIISERERERGEKKKKMKTETPPHTHPHITILTDVLPSVFVYIADMEETYDPTGQTCYMEACDKLQVVPASYFLRHMHSNELSMAHRNLGPKGTKALAVPLVTNTSILKLNLRDNWMEGMGGAAIAEMLKENCYITGGFSGHSSMHTFSPSSLCHLRLTKVPPHSHSYATLTPIICHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLQ9</accession>
    <name>A0A672YLQ9_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Outer dense fiber protein 3-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">odf3l2a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YLQ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005005600.1">
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        <property type="gene ID" value="ENSSORG00005003234.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLQ9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF57">
        <property type="entry name" value="OUTER DENSE FIBER OF SPERM TAILS 3-LIKE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="263" mass="28861" checksum="DD916109835E5E39" modified="2020-06-17" version="1">TIENKPTEQQGLNVLHHFHSCRHGPGLVWFSGPGPGRYALPPTVGYINHDFTKPSSPAYSFHSLVSVDSSPGPRYNIDAKVTRFGRMDTPSYSILSCTLLGQVFQTPGPGTYSPEKAPPVNTHRRPPSYTMSTRTRYRAVDAVPAPNSYMLPNLLGCQVPNKPSSASYSFLGRRNVGAPSEDLSKSPGPGKYNSTPADIYRTRQPSFSMQSRTKRPSYSSSIPGPGTYSPEKFYMHLPKPPSFTLGIRHSEFVTPLVVDVVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNK2</accession>
    <name>A0A672YNK2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoglycerate mutase</fullName>
            <ecNumber evidence="10">5.4.2.11</ecNumber>
            <ecNumber evidence="10">5.4.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pgam1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(2R)-3-phospho-glyceroyl phosphate = (2R)-2,3-bisphosphoglycerate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17765"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="EC" id="5.4.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="10"/>
    <dbReference type="EC" id="5.4.2.4" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672YNK2"/>
    <dbReference type="Ensembl" id="ENSSORT00005006387.1">
        <property type="protein sequence ID" value="ENSSORP00005006135.1"/>
        <property type="gene ID" value="ENSSORG00005003645.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YNK2"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004082">
        <property type="term" value="F:bisphosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000007">
        <property type="entry name" value="Phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010713">
        <property type="entry name" value="PRK14115.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="7">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="116"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="254" mass="28942" checksum="E35DFA0397D5A2B7" modified="2020-06-17" version="1">MAAYKLVLIRHGESCWNQENRFCGWFDADLSETGEMEAKRGGQALKDAGYEFDICYTSVLKRAIRTLWHVLDSIDQMWLPVHRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSFDTPPPPMDEAHDYYQTISKDRRYADLTEDQLPTCESLKDTIARALPFWEEEIVPQIKDGKRVLIAAHGNSLRGIVKHLESMSEEAIMELNLPTGIPIVYELDKNLKPVGPMQFLGDEETVKKAMEAVAAQGKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPM1</accession>
    <name>A0A672YPM1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serotransferrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sxph</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Transferrins are iron binding transport proteins which bind Fe(3+) ion in association with the binding of an anion, usually bicarbonate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transferrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPM1"/>
    <dbReference type="Ensembl" id="ENSSORT00005006805.1">
        <property type="protein sequence ID" value="ENSSORP00005006546.1"/>
        <property type="gene ID" value="ENSSORG00005003836.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPM1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13529">
        <property type="entry name" value="PBP2_transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016357">
        <property type="entry name" value="Transferrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001156">
        <property type="entry name" value="Transferrin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485:SF28">
        <property type="entry name" value="OVOTRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485">
        <property type="entry name" value="TRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00405">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002549">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00422">
        <property type="entry name" value="TRANSFERRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00094">
        <property type="entry name" value="TR_FER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51408">
        <property type="entry name" value="TRANSFERRIN_LIKE_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Transferrin-like" evidence="8">
        <location>
            <begin position="25"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="8">
        <location>
            <begin position="368"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="135"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="178"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="429"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="485"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="513"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="536"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="614"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="732" mass="80751" checksum="7905625D00D89ECE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPT6</accession>
    <name>A0A672YPT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 23">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="5 23">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">nlk1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 23">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="23">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="5 23"/>
    <dbReference type="AlphaFoldDB" id="A0A672YPT6"/>
    <dbReference type="FunCoup" id="A0A672YPT6">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006871.1">
        <property type="protein sequence ID" value="ENSSORP00005006611.1"/>
        <property type="gene ID" value="ENSSORG00005003762.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPT6"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07853">
        <property type="entry name" value="STKc_NLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000162">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000164">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="88"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="475" mass="52549" checksum="C227C954D353C0CE" modified="2020-06-17" version="1">MAFHGAGRQTVCGDLFPGSDLGHKYFCVNSSCGAPSTGLSATPCLTGPTAPAGTPRHPTALGGSAGGGAAVPQPYSNPASEVPSPAEMEPDRPIGYGAFGVVWSVTDPRDGRKVALKKMPNVFQNLVSCKRVFRELRMLCFFKHDNVLSALDILQPPQIDCFEEIYVITELMQSDLHKVIVSPQPLTTDHIKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCLLKICDFGLARVEEPDPSRHMTQEVVTQYYRAPEVLMGCRHYNSAIDVWSVGCIFAELLGRRILFQAQSPIQQLDLITDLLGTPPLSALSSACEGARAHILRGPHKPPSLSVLYMLSDGATHEAVHLLCRMLVFDPAKRISGSDALSHPYLDEGRLRYHTCMCQCCYSVPSGRVYTRDFEPVAERPFSHSYENSLLSVWQGKELIHRFITEHQQGKRVPLCINPQSAAFKTFIRSTAWHSSKVSRKEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQN3</accession>
    <name>A0A672YQN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">itgb6</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="20">Heterodimer of an alpha and a beta subunit. Interacts with FLNB. Interacts with HAX1. ITGAV:ITGB6 interacts with FBN1. ITGAV:ITGB6 interacts with TGFB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 22">Cell membrane</location>
            <topology evidence="1 22">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YQN3"/>
    <dbReference type="FunCoup" id="A0A672YQN3">
        <property type="interactions" value="711"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007094.1">
        <property type="protein sequence ID" value="ENSSORP00005006832.1"/>
        <property type="gene ID" value="ENSSORG00005003962.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YQN3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034685">
        <property type="term" value="C:integrin alphav-beta6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
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        <text evidence="12">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
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        <text evidence="11">Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Interacts with LRWD1 predominantly during the G1 phase and with less affinity during mitosis, when phosphorylated.</text>
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        <property type="entry name" value="Cdc6_C"/>
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    </dbReference>
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        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 1"/>
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    </dbReference>
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        <source>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006925.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="829" mass="91316" checksum="695F26DDD2CCD1EA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRF4</accession>
    <name>A0A672YRF4_9TELE</name>
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        </recommendedName>
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        <name evidence="4" type="primary">LOC115411499</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <proteinExistence type="predicted"/>
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    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="6"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="157" mass="17556" checksum="3D6D38F870B56B6D" modified="2020-06-17" version="1">VCSSSLANNQLTSLPVSLSNLTRLRKLNLSHNLIAHIPACVYNMKALVFLHLAYNRLENLAENIQALVELKILIVEGNSLHCLPKALCCLTRLELLNLNFNDIKDVPQEMHQLSRLEKLACHPLDKGLHILHNPLLKPIKEVLEGGLSALFNYLRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSA7</accession>
    <name>A0A672YSA7_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">[histone H3]-lysine(36) N-trimethyltransferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">setd2</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSSORG00005001351.1"/>
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    <dbReference type="InParanoid" id="A0A672YSA7"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:histone H3K36 trimethyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Set2, Rpb1 interacting domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AWS_dom"/>
    </dbReference>
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        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
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        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044437">
        <property type="entry name" value="SETD2/Set2_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042294">
        <property type="entry name" value="SETD2_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013257">
        <property type="entry name" value="SRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038190">
        <property type="entry name" value="SRI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46711">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46711:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17907">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WW"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YW26</accession>
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            <taxon>Neoteleostei</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        </source>
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        </source>
    </evidence>
    <sequence length="990" mass="111950" checksum="833E6C174A7A4468" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWE0</accession>
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        <recommendedName>
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        <dbReference type="NCBI Taxonomy" id="375764"/>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="NLR"/>
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        <property type="match status" value="1"/>
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            <end position="82"/>
        </location>
    </feature>
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        </location>
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            <end position="72"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40393" checksum="4849C6B6CE35ECBB" modified="2020-06-17" version="1">MSLSVDEEEDGAELSHYTNRGLSIKEPPGSSNEPGPSDPQGQYNRPRAESSVSTCLSMKTVWSKDDPPDFRPEPGPSDTEFSRCGLSETHCEVVASALKSNPSHLRLLHLSENNLRDSGAKLASGLKSPNCRLEILGMRRCSLSEASCSSVASALKSNPFHLIKLDLSGNELKDSSAKELCDFLQSPHCKLQELRLNKCSLPEVSCSVASALKSNPSHLIKLSLGGNNLQDSSVEELCDFLQSPHCKLQELWLSKCSLSEIRCSSVVSALKSNPFHLIKLNLGGNNLQDSGVKELCDFLQSPHCKLQDLRLWGCNLSKISCSYLAAVLKSNPSHLIQLDLSQNNLKDSDVKELGDLVQSPHCKLEKLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWK4</accession>
    <name>A0A672YWK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YWK4"/>
    <dbReference type="Ensembl" id="ENSSORT00005009231.1">
        <property type="protein sequence ID" value="ENSSORP00005008929.1"/>
        <property type="gene ID" value="ENSSORG00005004924.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YWK4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF14">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="2"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="195" mass="22140" checksum="2BA093AB689925F9" modified="2020-06-17" version="1">SCCLEEVKKHVVLLCASDACDLTLDPNTVNQSLALSDDNRTVTETKEKLPYPNHPERFNHWKQVLCKEGLTGRCYWEVECKGWVNIGVAYKGIKRKGQDDDCWIGQNDKSWSLMCCDDKFTACHKNTRTVLRTPPALDCGRVGVYLDCLAGTLSFYRVSSDTLIHLHTFHTTFTEPLYAAFGFEFINFSVTLCQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2L2</accession>
    <name>A0A672Z2L2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 687a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">znf687a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005011328.1">
        <property type="protein sequence ID" value="ENSSORP00005010954.1"/>
        <property type="gene ID" value="ENSSORG00005005930.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2L2"/>
    <dbReference type="OrthoDB" id="7312725at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045914">
        <property type="entry name" value="Zn532-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041697">
        <property type="entry name" value="Znf-C2H2_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057356">
        <property type="entry name" value="Znf-C2H2_ZNF592"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222">
        <property type="entry name" value="ZINC FINGER PROTEIN 532-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16622">
        <property type="entry name" value="zf-C2H2_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25412">
        <property type="entry name" value="zf-C2H2_ZNF592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="664"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="929"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="960"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1065"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1224"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1300"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="481"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="589"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="983"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1124"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1261"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="355"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="381"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="435"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="518"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1015"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1134"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1279"/>
            <end position="1292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1336" mass="145220" checksum="0144939A5428514E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2T8</accession>
    <name>A0A672Z2T8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z2T8"/>
    <dbReference type="Ensembl" id="ENSSORT00005011900.1">
        <property type="protein sequence ID" value="ENSSORP00005011516.1"/>
        <property type="gene ID" value="ENSSORG00005006157.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2T8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10068">
        <property type="entry name" value="BONE MARROW PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10068:SF14">
        <property type="entry name" value="CELL WALL ADHESIN EAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="130" mass="13390" checksum="37DFB59A3BD8A222" modified="2020-06-17" version="1">DWITLYMNEASIEPMGPVEPVGPVEPVGPVDPVGPVDPVEPMGPVEPVEPMGPMEPVEPMGPMGPVEPVGPEEPVGPVEVVEPMRPVEPMGPVESMGPVEPVGPVEPMGPIGPVEPVGPVEPVGPVEPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z435</accession>
    <name>A0A672Z435_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z435"/>
    <dbReference type="Ensembl" id="ENSSORT00005012398.1">
        <property type="protein sequence ID" value="ENSSORP00005012006.1"/>
        <property type="gene ID" value="ENSSORG00005006368.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z435"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF249">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 6 MEMBER 22, TANDEM DUPLICATE 2 ISOFORM X2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="172" mass="19466" checksum="462109C05A89992C" modified="2020-06-17" version="1">MESTNNPEKNLMRDGNSPHQLKEKTKQRGQWANKREFLLAVIGEIIGLGNVWRFPYLCFKNGGGVFLVPYILFLVTCGIPIFFLEVSVGQLTGQGGVTCWRKICPLFEGLGYGSQVVMVYTVIYYIVILAWAFLYLFSSFTSELPWARCNNTWNTGTNYNLHVDTEQTDSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z549</accession>
    <name>A0A672Z549_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein strawberry notch homolog 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">sbno2b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a transcriptional coregulator, that can have both coactivator and corepressor functions. Inhibits the DCSTAMP-repressive activity of TAL1, hence enhancing the access of the transcription factor MITF to the DC-STAMP promoter in osteoclast. Plays a role in bone homeostasis; required as a positive regulator in TNFSF11//RANKL-mediated osteoclast fusion via a DCSTAMP-dependent pathway. May also be required in the regulation of osteoblast differentiation. Involved in the transcriptional corepression of NF-kappaB in macrophages. Plays a role as a regulator in the pro-inflammatory cascade.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with TAL1; this interaction inhibits TAL1 occupancy of the DCSTAMP promoter, leading to the activation of the DCSTAMP promoter by the transcription factor MITF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005012803.1">
        <property type="protein sequence ID" value="ENSSORP00005012406.1"/>
        <property type="gene ID" value="ENSSORG00005005374.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z549"/>
    <dbReference type="OrthoDB" id="421838at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071354">
        <property type="term" value="P:cellular response to interleukin-6"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002281">
        <property type="term" value="P:macrophage activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000342">
        <property type="entry name" value="Protein strawberry notch homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003990">
        <property type="entry name" value="Si:ch73-63e15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057332">
        <property type="entry name" value="SBNO_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF5">
        <property type="entry name" value="PROTEIN STRAWBERRY NOTCH HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25373">
        <property type="entry name" value="SBNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Strawberry notch AAA" evidence="14">
        <location>
            <begin position="236"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Strawberry notch helicase C" evidence="13">
        <location>
            <begin position="735"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="domain" description="SBNO alpha/beta" evidence="15">
        <location>
            <begin position="1050"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="189"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="637"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1331"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1499"/>
            <end position="1524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1220"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="647"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="667"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1331"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1342"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1505"/>
            <end position="1524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1611" mass="179114" checksum="7517D216B0462048" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z670</accession>
    <name>A0A672Z670_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Olfactomedin 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z670"/>
    <dbReference type="Ensembl" id="ENSSORT00005012706.1">
        <property type="protein sequence ID" value="ENSSORP00005012309.1"/>
        <property type="gene ID" value="ENSSORG00005006476.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z670"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022082">
        <property type="entry name" value="Noelin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF80">
        <property type="entry name" value="OLFACTOMEDIN 2 LIKE PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12308">
        <property type="entry name" value="Noelin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="10">
        <location>
            <begin position="203"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="204"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="449" mass="50546" checksum="1A19B21E4EC7EBC6" modified="2020-06-17" version="1">MSQTLPSLVGLNGTIISSEGTHERIVSGLYPGSEEGWQVYSTASDPDGRCVCTVVAPARNLCKRDPRSRQLRLLTEQVQNVSQSMEVVDLRTSRDLQYVRDSEPLVRGVDGRLHTYVANPRSLTAKGLQELKGQMSQLRPLLSVVEQYRLDLQTLATLRLELLNLSIILTAIQEEIGAYDYEELQQRVLLLETRLHSCMNKLGCGRLTSVSSPITVRASGSRFGSWMTDAMIPSSDSRVWSMDGYYKGRRVLEYRTMGDFTKGQNFVQHLLPHPWAGTGHVVYNGSLYYNKHQSNILVQYHFRSRSVLLQRSLSGAGYNNTFPYSWGGSSDIDLMADEMGLWAVYTSIPNAGNIMVSRLDPRSLDVLQSWDTGFPKRSAGEAFMICGTLYVTNSHLAGAKVHFAYHTNTSTYEYTDIPFHNQALYTWNNGHQVIYNVTLFHVIRSDDPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7Q7</accession>
    <name>A0A672Z7Q7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 206</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for sperm motility and is involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the respiratory tract. Required for the establishment of radial spokes in sperm flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CFAP206 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z7Q7"/>
    <dbReference type="Ensembl" id="ENSSORT00005013816.1">
        <property type="protein sequence ID" value="ENSSORP00005013406.1"/>
        <property type="gene ID" value="ENSSORG00005006932.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z7Q7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003356">
        <property type="term" value="P:regulation of cilium beat frequency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901317">
        <property type="term" value="P:regulation of flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021897">
        <property type="entry name" value="FAP206"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21442">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21442:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12018">
        <property type="entry name" value="FAP206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="232"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="297" mass="33485" checksum="C0762A545C05E913" modified="2020-06-17" version="1">SLVSSLCSKVLIFGSSWFQPLFQSLSQVWSGLQDEAELLHILSLHSPSVFLVHVFISWCLVWGSAERIDPAQMKTQEWLFPENLSNFDQVLLQFNGFCGYTLVERDGLLLPGDPHIGILKHQQKLFVFSSTDAAVHFSAAPDQFISEVAEKAKRSPELIQLLRLHQQFPSVSPYSEMKPGDSFVAKPISKCESGTQTDVHPLETNICRTYQWNQWELRRRAIKLADLRTRVTVSTQTPQSHMRRDNATQTWTPKDAESQTQREGGSGVPRPQVYLAGLRGQRDGPAVKVNLTTPVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z978</accession>
    <name>A0A672Z978_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phospholipase</fullName>
            <ecNumber evidence="10">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pld1b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z978"/>
    <dbReference type="Ensembl" id="ENSSORT00005013815.1">
        <property type="protein sequence ID" value="ENSSORP00005013405.1"/>
        <property type="gene ID" value="ENSSORG00005001714.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z978"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032534">
        <property type="term" value="P:regulation of microvillus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000114">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000005">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000009">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000033">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF57">
        <property type="entry name" value="PHOSPHOLIPASE D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="85"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="854"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="525"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="551"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1037" mass="118600" checksum="0AFF88C8FEB6AF60" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z993</accession>
    <name>A0A672Z993_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cyclin-dependent kinase-like 2</fullName>
            <ecNumber evidence="6">2.7.11.22</ecNumber>
            <ecNumber evidence="5">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="6"/>
    <dbReference type="EC" id="2.7.11.24" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z993"/>
    <dbReference type="Ensembl" id="ENSSORT00005014349.1">
        <property type="protein sequence ID" value="ENSSORP00005013931.1"/>
        <property type="gene ID" value="ENSSORG00005007133.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z993"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000049">
        <property type="entry name" value="cyclin-dependent kinase-like 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF241">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="4"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="302" mass="34868" checksum="D378F0F3BD3783BF" modified="2020-06-17" version="1">MERYQSLGLVGEGSYGTVLKCRHRGSGRLVAIKKFLDSDDDRTVKKIAQREIKLLRQLRHDNLVNLLEVWKRRRRWYLVFEFVERTLLDELEQNPNGLDLNTSRQYLFQILRAGAFCHQQNIIHRDIKPENILISHSGVIKLCDFGFARTVASPAEGGVYTDYVATRWYRPPELLVGDTKYGKPVDVWAVGCLFMEMLTSQPLFPGDSDLDQIHHIVRCFGENHRFSVCALPKMLQNFHSRVLLLYTTFSGTFHELTCSFCPPRFVTLHNFMCSFDGLMCSFFCGSNKKSTTLLQASHYTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCA8</accession>
    <name>A0A672ZCA8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fbxo48</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997809.1">
        <property type="nucleotide sequence ID" value="XM_030141949.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029997818.1">
        <property type="nucleotide sequence ID" value="XM_030141958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZCA8"/>
    <dbReference type="FunCoup" id="A0A672ZCA8">
        <property type="interactions" value="516"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014550.1">
        <property type="protein sequence ID" value="ENSSORP00005014128.1"/>
        <property type="gene ID" value="ENSSORG00005007239.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424590"/>
    <dbReference type="CTD" id="554251"/>
    <dbReference type="InParanoid" id="A0A672ZCA8"/>
    <dbReference type="OrthoDB" id="10257471at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="31"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="154" mass="17901" checksum="B736D405D7F6E140" modified="2020-06-17" version="1">MQHDALRTSPVFFLCEGKCILSSSTDNRTPQNFAETLPTEMSVRIFGELDTESLCSASRTCKLWHDIIEESDQLWRRQCLLVRGVCQREVDRDRRDGLSWKVTLVRNYTRSCLKRDWLRGRYSNVASANELSGRRMTPLDAETWGEILQAELDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDR8</accession>
    <name>A0A672ZDR8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane serine protease 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDR8"/>
    <dbReference type="Ensembl" id="ENSSORT00005015983.1">
        <property type="protein sequence ID" value="ENSSORP00005015491.1"/>
        <property type="gene ID" value="ENSSORG00005007855.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDR8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="138"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="381" mass="41562" checksum="A63B2EA62E7B1E4E" modified="2020-06-17" version="1">RFKMLKVSDVEVSHSPSKVQFQLGLSCVGKFRCGLSSKCIPSSAQCDGKFQCDNGEDELGCGETQTQQHINKHNNTQTHNTQMQQSIFGFISEFVSDVESNFLLLSSMSLHKSQCNSGKVTTLKCLECGSRPRFTTRIVGGNLSRPGQFPWQVSLHIGGEHLCGGSIITSRWIVTAAHCMEGFADPSIWGVHVGLTELPAHLAQSLSVEQILYHAKYRRSKLDYDIALIKLKTPLVFNGLIQPICLPNHGEQFEEGTMCWISGWGSTDDGGESSIVLRSAMVPLLSTKTCSQPDVYPGLISPWMICAGYLGGGIDSCQGDSGGPLACEDSSTWKLVGATSWGIGCGDRNRPGVYTRITQSLNWIRQQMGVSTCYSHRVKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFM4</accession>
    <name>A0A672ZFM4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pitx2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000964.1">
        <property type="nucleotide sequence ID" value="XM_030145104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZFM4"/>
    <dbReference type="FunCoup" id="A0A672ZFM4">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016123.1">
        <property type="protein sequence ID" value="ENSSORP00005015629.1"/>
        <property type="gene ID" value="ENSSORG00005007934.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426872"/>
    <dbReference type="CTD" id="5308"/>
    <dbReference type="InParanoid" id="A0A672ZFM4"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021536">
        <property type="term" value="P:diencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000031">
        <property type="entry name" value="Homeobox protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016233">
        <property type="entry name" value="Homeobox_Pitx/unc30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882:SF4">
        <property type="entry name" value="PITUITARY HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000563">
        <property type="entry name" value="Homeobox_protein_Pitx/Unc30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="81"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="14">
        <location>
            <begin position="275"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="83"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="49"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="313" mass="35138" checksum="3A099710B59E31FB" modified="2020-06-17" version="1">MNSMRDPLNIDHHHITGNKLASTHHTALAMASSLQPLQRSVDSKHRLEVHTVSDTSSPESVEKEKSQNKNDDSSDDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPSYTYNNWAAKGLTSASLSTKSFPFFNSMNVNPLSSQTMFSPPNSISSMTSSMVPSAVTGVPGSSLNSLNNLNNLSNPSLNSGVPTPTCPYAPPTPPYVYRDTCNSSLASLRLKAKQHSSFGYASVQNPATNLSACQYAVDRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHR9</accession>
    <name>A0A672ZHR9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZHR9"/>
    <dbReference type="FunCoup" id="A0A672ZHR9">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016806.1">
        <property type="protein sequence ID" value="ENSSORP00005016299.1"/>
        <property type="gene ID" value="ENSSORG00005008263.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZHR9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000112">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000127">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.30">
        <property type="entry name" value="DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042521">
        <property type="entry name" value="DYRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="104"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="459" mass="53584" checksum="C16265804197CCA0" modified="2020-06-17" version="1">MRFGATMDKVSVSRNSKIFSNKLEKEKAYEGQRLPMTPTEALKNFQDRLTEFEQEEIMDYSEIWFLGLGSQKIEGSQSSPQNSGYDDEHGSYVRVLHDHIAYRFEVLEVIGKGSFGQVLKCLDHKNNELVAIKMIRNKKRFHHQALVELKILDVIKRKDKDNLHNVIHMKEYFYFRNHLCISFELLGVNLYELIKKNNFQGFSLALIRRFAHALLRCLQMLHREKIIHCDLKPENILLSQRGPGNIKVVDFGSSCYEQQRVYTYIQSRFYRSPEVILGHPYSMAIDMWSLGCILAELYTGYPLFPGESEVEQIACIMEVLGMPPNDFVQSASRRRLFFDSKGNPRNITNSKGKKRRPSSKELSLALKTNDALFLDFIQRCLTYVHSVLLSHIYLTYTSLKCDKRHNFAFQEEERWEKHIYFCKHSSCLRGHVLHQDLFCACGSLQGHIQFILKTDRSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHS1</accession>
    <name>A0A672ZHS1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Interferon-induced GTP-binding protein Mx</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Interferon-inducible Mx protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115420170</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
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    <dbReference type="FunCoup" id="A0A672ZHS1">
        <property type="interactions" value="22"/>
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        <property type="protein sequence ID" value="ENSSORP00005016018.1"/>
        <property type="gene ID" value="ENSSORG00005007121.1"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Interferon-induced GTP-binding protein Mx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000621">
        <property type="entry name" value="Interferon-induced GTP-binding protein Mx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
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        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
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        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
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        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
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        <property type="entry name" value="GED"/>
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        <property type="entry name" value="GED_dom"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF225">
        <property type="entry name" value="INTERFERON-INDUCED GTP-BINDING PROTEIN MX-RELATED"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DYNAMIN"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="9">
        <location>
            <begin position="30"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="8">
        <location>
            <begin position="541"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="666" mass="75700" checksum="8742A39E281067CC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJS9</accession>
    <name>A0A672ZJS9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Relaxin family peptide receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rxfp1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005017029.1"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000023">
        <property type="entry name" value="Relaxin family peptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000434">
        <property type="entry name" value="Relaxin family peptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008112">
        <property type="entry name" value="Relaxin_rcpt"/>
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    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF68">
        <property type="entry name" value="RELAXIN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01739">
        <property type="entry name" value="RELAXINR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="530"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="586"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="617"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="378"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="724" mass="82930" checksum="5CA3A55538EF6BE9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZK54</accession>
    <name>A0A672ZK54_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aquaporin 10b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115435816</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glycerol(in) = glycerol(out)</text>
            <dbReference type="Rhea" id="RHEA:29675"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZK54"/>
    <dbReference type="Ensembl" id="ENSSORT00005017674.1">
        <property type="protein sequence ID" value="ENSSORP00005017159.1"/>
        <property type="gene ID" value="ENSSORG00005008587.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZK54"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF13">
        <property type="entry name" value="AQUAPORIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02019">
        <property type="entry name" value="AQUAPORIN7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="302" mass="32385" checksum="6E4FA8C043AFECC9" modified="2020-06-17" version="1">KMKVSPIISQIGSFFTLRTLFYGTIYKFSLIGSGAAAQVITSQGTHGSYLTMQLGSALGAVFGIFVARGVSGAQLNVVWSMSLWLLGRQPWRKVPLYALSHLLGAFLGAATVYLQYYDAIQVFSGGHLSVAGPNATAGIFCSYPADHLSVWGGIVDQVVATALLLICCLAVEDRRQGLLPDGLAPVLVGALILTIGLSMGSNCGFPLNPARDLGPRLFTFIAGWGEEVFKAGGGWWWVPIVAPFVGGLLGTLIYELLIEVHHPHTPQSPGQESEAVELEEVVVDSGNNRKTEVYLEELMNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZN52</accession>
    <name>A0A672ZN52_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pro-opiomelanocortin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115414947</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Stimulates the adrenal glands to release cortisol.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the POMC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984177.1">
        <property type="nucleotide sequence ID" value="XM_030128317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZN52"/>
    <dbReference type="Ensembl" id="ENSSORT00005018786.1">
        <property type="protein sequence ID" value="ENSSORP00005018244.1"/>
        <property type="gene ID" value="ENSSORG00005009044.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414947"/>
    <dbReference type="InParanoid" id="A0A672ZN52"/>
    <dbReference type="OrthoDB" id="8962839at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013531">
        <property type="entry name" value="Mcrtin_ACTH_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013593">
        <property type="entry name" value="Melanocortin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001941">
        <property type="entry name" value="PMOC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050878">
        <property type="entry name" value="POMC-derived_peptides"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11416">
        <property type="entry name" value="PRO-OPIOMELANOCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11416:SF7">
        <property type="entry name" value="PRO-OPIOMELANOCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00976">
        <property type="entry name" value="ACTH_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08384">
        <property type="entry name" value="NPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00383">
        <property type="entry name" value="MELANOCORTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01363">
        <property type="entry name" value="ACTH_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025370126" evidence="8">
        <location>
            <begin position="24"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Pro-opiomelanocortin/corticotropin ACTH central region" evidence="9">
        <location>
            <begin position="98"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Pro-opiomelanocortin/corticotropin ACTH central region" evidence="9">
        <location>
            <begin position="181"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="230" mass="25866" checksum="16F515ADC9E976D8" modified="2020-06-17" version="1">MVCPSWLVMVVMAHVCVPGFGSACWDNSICNDLSNKERILECIHVCMSVIQNEFPKLTSLKVNSEDDDVLLSILLNSVAPGDKTLEPRLRAHSNERRSYSMEHFRWGKPPARKRRPIKVFASSLEGGGSSEGSFPLQARRQLGSNEDDLSSDRQQTQGLARMMVSSKSNAPFSPQQRKDGTYRMSHFRWGSPPASKRNANFMKLGEEKPQGQLAKLFRNIVVKDLQRIMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZNF8</accession>
    <name>A0A672ZNF8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Complexin 4c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cplx4c</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZNF8"/>
    <dbReference type="Ensembl" id="ENSSORT00005018898.1">
        <property type="protein sequence ID" value="ENSSORP00005018354.1"/>
        <property type="gene ID" value="ENSSORG00005009084.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZNF8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22809">
        <property type="entry name" value="Complexin_NTD_CPLX_III_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF12">
        <property type="entry name" value="COMPLEXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="144" mass="16663" checksum="15157109F690FC05" modified="2020-06-17" version="1">MAFLMNQMLGDKLKNFTGGETPESKGMSREEFEEYQKQLVEEKIQRDQDFATKKAERANLRVALRDKYRLPEVRSLVTVYTMAGDDIDVPEELAKMVDEDAEEEEVNDSFLKKLQNMDVDALKTKAQTTMTEMKHTAEEKCILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZRM2</accession>
    <name>A0A672ZRM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-sarcoglycan</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">sgcb</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Cross-link to form 2 major subcomplexes: one consisting of SGCB, SGCD and SGCG and the other consisting of SGCB and SGCD. The association between SGCB and SGCG is particularly strong while SGCA is loosely associated with the other sarcoglycans.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <location evidence="3">Sarcolemma</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sarcoglycan beta/delta/gamma/zeta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZRM2"/>
    <dbReference type="FunCoup" id="A0A672ZRM2">
        <property type="interactions" value="804"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020073.1">
        <property type="protein sequence ID" value="ENSSORP00005019514.1"/>
        <property type="gene ID" value="ENSSORG00005009592.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZRM2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006875">
        <property type="entry name" value="Sarcoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027659">
        <property type="entry name" value="Sgcb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21142:SF2">
        <property type="entry name" value="BETA-SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21142">
        <property type="entry name" value="SARCOGLYCANS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04790">
        <property type="entry name" value="Sarcoglycan_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="321" mass="35781" checksum="40B61DCCC15B4056" modified="2020-06-17" version="1">MEFCWVCKQMLFFQESSNGPVKKSMREKAIERRTINKEHNSNFKAGYIPIEEERLHKTGLRGRKGNMAVCIIVLLFLLALINLIITLVIWTVIRIGPNGCDSMEFHESGLLRFKQKADMGIVHPLHKSTVGGRKDQDLVIVGNNNPVVFQQGTTKLSVEKDKTSVVSDIGISFTDPRTQNTFFSTDFENHEFHLPKGVKVLSVKKASTERITSSAASDLNIKGDGKAIIRGNEGVNIMGRTVEFKMGGGIELRAENSIVLNGSVMFNATRIPNSAGDVYFDEGLERYKLCMCADGTLFRVQVKFPNMGCQTSDNPCKKAHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUL2</accession>
    <name>A0A672ZUL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DEP domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115421000</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEPDC7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZUL2"/>
    <dbReference type="Ensembl" id="ENSSORT00005021597.1">
        <property type="protein sequence ID" value="ENSSORP00005020970.1"/>
        <property type="gene ID" value="ENSSORG00005010241.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZUL2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04405">
        <property type="entry name" value="RhoGAP_BRCC3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206">
        <property type="entry name" value="DEP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206:SF9">
        <property type="entry name" value="DEP DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025517561" description="DEP domain-containing protein 7" evidence="4">
        <location>
            <begin position="21"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="5">
        <location>
            <begin position="42"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="496" mass="55228" checksum="5748F7CC0C16A44B" modified="2020-06-17" version="1">KTSAILTISCLGLSVYHVHMSSGVAVKPVQSTLLWSSLISHLRSNVTVKRRRVHIKSHSDCFLGSEAVDVVSKLCIFFKIEDADVTRDKVVCVCQALLDCSVFEAVGTKVFGKDKKQVVFQDSKSALYRLDTTVKFNRLNTPTADSSSLEPPVDGPSPSPSASLSPMVDEVWQEQTLLHLLKLVELPLLDGVLQCSQKTPSPCKTHTLVHNNPDLIYSSNHLDRQILKAFRDSQEDEWLCAALDCLDFLPDQPVVELSRGLPHCFPEAPGSCNQCKQLLYETLVKHYSHSDQPPLLPQHMTDVYTAITDLLVNAKLATALEALQLCLKLLRPGCREELRRLLTFMALAAEPQGIKLDKEVENRIAVKKSFSKAILHSKGLSKEKEDLMVVFMLSNIQEIFKIPGALHKEVSDKLASLVQGKQPDVTGPTLCQQVSSRTYSASTKTNTNQELWTLLDSIHSDSKLSSKQRKHLLEQFYQAHPEVFNQYFGDSAVSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWJ5</accession>
    <name>A0A672ZWJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115416808</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the spectrin family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A672ZWJ5">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021210.1">
        <property type="protein sequence ID" value="ENSSORP00005020633.1"/>
        <property type="gene ID" value="ENSSORG00005010030.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZWJ5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
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    <sequence length="1675" mass="185771" checksum="6601BD4EBE08EDE2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZX04</accession>
    <name>A0A672ZX04_9TELE</name>
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        <recommendedName>
            <fullName evidence="16">Glutamine--tRNA ligase</fullName>
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        <alternativeName>
            <fullName evidence="12">Glutaminyl-tRNA synthetase</fullName>
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        <name evidence="23" type="primary">qars1</name>
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    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="14">Glutamine--tRNA ligase. Plays a critical role in brain development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
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        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with RARS1. Part of a complex composed of RARS1, QARS1 and AIMP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
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        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
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        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
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    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
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    <keyword evidence="6 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
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        </location>
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    </feature>
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            <begin position="566"/>
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    </feature>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="776" mass="87804" checksum="C01B322114F79D55" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXB9</accession>
    <name>A0A672ZXB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FXYD domain-containing ion transport regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FXYD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZXB9"/>
    <dbReference type="Ensembl" id="ENSSORT00005022305.1">
        <property type="protein sequence ID" value="ENSSORP00005021659.1"/>
        <property type="gene ID" value="ENSSORG00005010594.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZXB9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017080">
        <property type="term" value="F:sodium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043269">
        <property type="term" value="P:regulation of monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20328">
        <property type="entry name" value="FXYD3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.780:FF:000008">
        <property type="entry name" value="FXYD domain-containing ion transport regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.780">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000272">
        <property type="entry name" value="Ion-transport_regulator_FXYD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14132:SF14">
        <property type="entry name" value="FXYD DOMAIN-CONTAINING ION TRANSPORT REGULATOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14132">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02038">
        <property type="entry name" value="ATP1G1_PLM_MAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="123" mass="13813" checksum="B22DFDB1F3C921A9" modified="2020-06-17" version="1">MSSVFFAAVPHVSSCPDFNPLSFVLCPTAEDDPFTFDYHRLRVGGLILAAVLCLIGITILLSGHCRCKFNQKRRSVDRRGRCATRRSGLSWTLKTFEKVENIPSQCSSDLRDTPYIWKKSASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZ35</accession>
    <name>A0A672ZZ35_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>riboflavin(in) = riboflavin(out)</text>
            <dbReference type="Rhea" id="RHEA:35015"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the riboflavin transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZZ35"/>
    <dbReference type="Ensembl" id="ENSSORT00005022224.1">
        <property type="protein sequence ID" value="ENSSORP00005021578.1"/>
        <property type="gene ID" value="ENSSORG00005010561.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZ35"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009357">
        <property type="entry name" value="Riboflavin_transptr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06237">
        <property type="entry name" value="SLC52_ribofla_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="71" mass="7958" checksum="62C8D7C30C610345" modified="2020-06-17" version="1">SASLIPDRPLLAFVMSLLTHVLLPLVVPAITEGWYLPSYLTVLIQMANIGPLLHHPHAPFSPREERPLLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZH5</accession>
    <name>A0A672ZZH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tumor suppressor 2, mitochondrial calcium regulator b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tusc2a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZZH5"/>
    <dbReference type="Ensembl" id="ENSSORT00005022365.1">
        <property type="protein sequence ID" value="ENSSORP00005021718.1"/>
        <property type="gene ID" value="ENSSORG00005010613.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZH5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051881">
        <property type="term" value="P:regulation of mitochondrial membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029393">
        <property type="entry name" value="FUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15453:SF9">
        <property type="entry name" value="TUMOR SUPPRESSOR 2, MITOCHONDRIAL CALCIUM REGULATOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15453">
        <property type="entry name" value="TUMOR SUPPRESSOR CANDIDATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15000">
        <property type="entry name" value="TUSC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14542" checksum="68C5B7A7979E529B" modified="2020-06-17" version="1">MGGSGSKAKGVWPFSGSGVGGDSACDGNEQSLARLKGSRNATPFVFTRRSSLYYDEDGDLAHEFYEETVVTKNGRKKSKLKRIQKNLIPQVSLAPGDRCYRYDFACMSCFLSTAIPIEITILCFILHYREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZW3</accession>
    <name>A0A672ZZW3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115414394</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZZW3"/>
    <dbReference type="Ensembl" id="ENSSORT00005023426.1">
        <property type="protein sequence ID" value="ENSSORP00005022760.1"/>
        <property type="gene ID" value="ENSSORG00005011050.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZW3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015732">
        <property type="term" value="P:prostaglandin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF86">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 3A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="609"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="9">
        <location>
            <begin position="446"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="693" mass="74888" checksum="2652C725F6770837" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4Y8</accession>
    <name>A0A673A4Y8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Selenoprotein H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">selenoh</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A4Y8"/>
    <dbReference type="Ensembl" id="ENSSORT00005025259.1">
        <property type="protein sequence ID" value="ENSSORP00005024545.1"/>
        <property type="gene ID" value="ENSSORG00005011801.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4Y8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052674">
        <property type="entry name" value="SelWTH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33638">
        <property type="entry name" value="SELENOPROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33638:SF1">
        <property type="entry name" value="SELENOPROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13541" checksum="400EA59EB90AD62E" modified="2020-06-17" version="1">MYYHLCRLGRRGTKRKAEVPAEEENPEVEEKKEGGAEENGPQGQRVIIEHWSGVKSALLAARPDLTVVLNPEKPRRNSFEITLLDGETSLWTGIKKGPPRKLKFPEPDVVVAALQEALKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5I3</accession>
    <name>A0A673A5I3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">E3 ubiquitin-protein ligase MARCHF2</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Membrane-associated RING finger protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Membrane-associated RING-CH protein II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase MARCHF2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">march2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673A5I3"/>
    <dbReference type="FunCoup" id="A0A673A5I3">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024605.1">
        <property type="protein sequence ID" value="ENSSORP00005023902.1"/>
        <property type="gene ID" value="ENSSORG00005011543.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A5I3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16808">
        <property type="entry name" value="RING_CH-C4HC3_MARCH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000119">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0458">Lysosome</keyword>
    <keyword evidence="17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
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            <begin position="140"/>
            <end position="162"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    <sequence length="248" mass="27448" checksum="120C56F7320F7AE5" modified="2020-06-17" version="1">MTTGGCCHLPGSLCDCASSTGLWKSVEEAGGDGCQAHYVTQVTAIDGRLLSSVLKPMSAQSDGPICRICHEGGNSEGLLSPCDCTGTLGTVHKSCLEKWLSSSNTSYCELCHTEFSIERRPRPLTEWLRDPGPRNEKRTLFCDMVCFLFITPLAAISGWLCLRGAQDHLQLGSWLQAVGLIALTIALFTIYVLWTLVSFRYHCQLYSEWRRTNQKVRLLIPEAKESHSSQHSLLSTKFMKKSANESIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A779</accession>
    <name>A0A673A779_9TELE</name>
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        <submittedName>
            <fullName evidence="13">Kinesin family member 5B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115437023</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
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    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CENTROMERE PROTEIN E"/>
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    </dbReference>
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        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
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        <property type="entry name" value="Kinesin"/>
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        <property type="entry name" value="KINESINHEAVY"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="957" mass="109724" checksum="A37F4CD6C34E391E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A9J3</accession>
    <name>A0A673A9J3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 94</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tmem94</name>
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    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
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            <taxon>Kurtiformes</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSSORP00005025257.1"/>
        <property type="gene ID" value="ENSSORG00005012072.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
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        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
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        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
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        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
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        <property type="entry name" value="TMEM94"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 94"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13219:SF6">
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    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
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        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="963"/>
            <end position="1165"/>
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        <location>
            <begin position="207"/>
            <end position="216"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1192" mass="132998" checksum="9469E1F8EE5E315B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="143" mass="15335" checksum="368EFA4AFBB6ADCE" modified="2025-10-08" version="2">MLDTDLPAMPEDANKDAMRTPAATPEKANNGEHPMANATTVNIPLVNEVQLTAATGGAELSCYRCTVPFGVVVLIAGIVVTAVAYSFNSHGSTISYFGLVLLSAGLVLLASSVVCWKVRMERKKERRRESQTALVANHRSIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIC3</accession>
    <name>A0A673AIC3_9TELE</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="8" type="primary">LOC115439272</name>
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    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="ENSSORG00005013592.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439272"/>
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    <dbReference type="OrthoDB" id="6778897at2759"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
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            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1285"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="276"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="403"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="576"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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        </location>
    </feature>
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        <location>
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            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1223"/>
            <end position="1249"/>
        </location>
    </feature>
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        <location>
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            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="782"/>
            <end position="797"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1319" mass="145119" checksum="9DD8E757C250A0E6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJ70</accession>
    <name>A0A673AJ70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dolichyldiphosphatase</fullName>
            <ecNumber evidence="11">3.6.1.43</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115430405</name>
        <name evidence="13" type="synonym">dolpp1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 11">Required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. Hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. Does not act on phosphatidate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a di-trans,poly-cis-dolichyl diphosphate + H2O = a di-trans,poly-cis-dolichyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14385"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="EC" id="3.6.1.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the dolichyldiphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.43" evidence="11"/>
    <dbReference type="RefSeq" id="XP_030006259.1">
        <property type="nucleotide sequence ID" value="XM_030150399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AJ70"/>
    <dbReference type="Ensembl" id="ENSSORT00005030226.1">
        <property type="protein sequence ID" value="ENSSORP00005029393.1"/>
        <property type="gene ID" value="ENSSORG00005014026.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030234.1">
        <property type="protein sequence ID" value="ENSSORP00005029401.1"/>
        <property type="gene ID" value="ENSSORG00005014029.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430405"/>
    <dbReference type="OrthoDB" id="302705at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047874">
        <property type="term" value="F:dolichyldiphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03382">
        <property type="entry name" value="PAP2_dolichyldiphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000003">
        <property type="entry name" value="Dolichyldiphosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039667">
        <property type="entry name" value="Dolichyldiphosphatase_PAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247:SF1">
        <property type="entry name" value="DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11247">
        <property type="entry name" value="PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="12">
        <location>
            <begin position="63"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="238" mass="27392" checksum="086241AEF2827951" modified="2020-06-17" version="1">MAMQEQCSAPPQWRSISLTHVEFAEGDLTGQVLAYITLLPIAILVGFVTLIVFKRELHTISFFGGIVLNEGVNWVLKHILREPRPCAGAHSSVPTEYGMPSSHSQLIWFFVVYFFLFLYLRMHQTNNARCVDLLWRHILSIILLGIALSVSYSRVYLLYHTWSQVFYGGVAGSTIGIIWFFFTQEVLTPLFPKIAAWPISEYFLVRDTSLIPNILWFEYTVTRSEARNRQRKLGTKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKW4</accession>
    <name>A0A673AKW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Molybdopterin synthase sulfur carrier subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AKW4"/>
    <dbReference type="Ensembl" id="ENSSORT00005030727.1">
        <property type="protein sequence ID" value="ENSSORP00005029889.1"/>
        <property type="gene ID" value="ENSSORG00005014274.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034986.1">
        <property type="protein sequence ID" value="ENSSORP00005034071.1"/>
        <property type="gene ID" value="ENSSORG00005016110.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990133">
        <property type="term" value="C:molybdopterin adenylyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00754">
        <property type="entry name" value="Ubl_MoaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044672">
        <property type="entry name" value="MOCS2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33359:SF1">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE SULFUR CARRIER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627724" description="Molybdopterin synthase sulfur carrier subunit" evidence="2">
        <location>
            <begin position="18"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="8957" checksum="5CA3B35D7D609F48" modified="2020-06-17" version="1">GVCLCCLHVCVLYFAQSADLTGSGGGVCAVPTPISSQDLWTLLLRRHPRLGAAASRGAGGASAVRCLGDHLLTLEDGDEVAVVPPLSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMA7</accession>
    <name>A0A673AMA7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cops3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AMA7"/>
    <dbReference type="FunCoup" id="A0A673AMA7">
        <property type="interactions" value="2060"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031235.1">
        <property type="protein sequence ID" value="ENSSORP00005030384.1"/>
        <property type="gene ID" value="ENSSORG00005014486.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AMA7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000354">
        <property type="entry name" value="COP9 signalosome complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000008">
        <property type="entry name" value="COP9 signalosome complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055089">
        <property type="entry name" value="COP9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048621">
        <property type="entry name" value="CSN3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF1">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22788">
        <property type="entry name" value="COP9_hel_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21215">
        <property type="entry name" value="CSN3-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="199"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="434" mass="49310" checksum="66176837552F48B5" modified="2020-06-17" version="1">MASALEQFVNNVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDIQEHSLGVLAVLFVKFSMPNIPDFETLFSQVQLFISTCNGEHIRYATDTCKSSLTHCLTCQMLKSFPLEKLGVVVLKQAIDKMQMNTNQLTSVHADLCQLCLLAKCFKPALPFLELDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLEAYKKYILVSLILHGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYTTNNPAELRSLVNKHSETFTRDNNTGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIYASINQKDGMVCFHDNPEKYNNPAMLHKIDQEMLKCIELDEKLKSMDQEITVNPQFVYAQVVHESQENMPNQVCSVVFSLSFCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AP02</accession>
    <name>A0A673AP02_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Immunoglobulin-like domain-containing receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115411785</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. LISCH7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979840.1">
        <property type="nucleotide sequence ID" value="XM_030123980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AP02"/>
    <dbReference type="FunCoup" id="A0A673AP02">
        <property type="interactions" value="696"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032287.1">
        <property type="protein sequence ID" value="ENSSORP00005031410.1"/>
        <property type="gene ID" value="ENSSORG00005014936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411785"/>
    <dbReference type="InParanoid" id="A0A673AP02"/>
    <dbReference type="OrthoDB" id="9944507at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070506">
        <property type="term" value="F:high-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051874">
        <property type="entry name" value="Ig-like_domain-LISCH7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008664">
        <property type="entry name" value="LISCH7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15923:SF3">
        <property type="entry name" value="IMMUNOGLOBULIN-LIKE DOMAIN-CONTAINING RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15923">
        <property type="entry name" value="TRANSMEMBRANE AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05624">
        <property type="entry name" value="LSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025397432" evidence="13">
        <location>
            <begin position="20"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="5"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="360"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="481"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="530" mass="58007" checksum="FA119E8F3F96CE82" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APX0</accession>
    <name>A0A673APX0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Metaxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mtx3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the metaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673APX0"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032334.1">
        <property type="protein sequence ID" value="ENSSORP00005031456.1"/>
        <property type="gene ID" value="ENSSORG00005014968.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APX0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:mitochondrion organization"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03212">
        <property type="entry name" value="GST_C_Metaxin1_3"/>
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    </dbReference>
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        <property type="entry name" value="GST_N_Metaxin1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="Metaxin1/3"/>
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    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
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    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF30">
        <property type="entry name" value="METAXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038150">
        <property type="entry name" value="Metaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="9">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="10">
        <location>
            <begin position="173"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="249" mass="27856" checksum="BFD2A4D373BF649C" modified="2020-06-17" version="1">MAAAMELRCWGGDWGLPSVHTESLIVLAYSKFSGAQVTVSPIDWTWKTLTATVPELVCGEAAVKDPNQILNFLRKQRFNADYELTARQGADTMAYIALLEEKLRPALLHTFWVDAENYANLTRPWFASRSPFPLNFVVPSRHANNALSRILLTKGESPLHRITEVEGKIYSDAKECLNLLSYRLGTANYFFGNSPTSLDAFVFGFVAPLHKASLPSSPLQSHLSQLDNLTRFCDNILALYFSSNHPCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AR27</accession>
    <name>A0A673AR27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Snurportin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA U transporter 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">snupn</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Functions as an U snRNP-specific nuclear import adapter. Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U snRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the snurportin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AR27"/>
    <dbReference type="FunCoup" id="A0A673AR27">
        <property type="interactions" value="1191"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031777.1">
        <property type="protein sequence ID" value="ENSSORP00005030913.1"/>
        <property type="gene ID" value="ENSSORG00005014739.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AR27"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061015">
        <property type="term" value="P:snRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09232">
        <property type="entry name" value="Snurportin-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017336">
        <property type="entry name" value="Snurportin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024721">
        <property type="entry name" value="Snurportin-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047857">
        <property type="entry name" value="Snurportin1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13403:SF6">
        <property type="entry name" value="SNURPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13403">
        <property type="entry name" value="SNURPORTIN1 RNUT1 PROTEIN RNA, U TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11538">
        <property type="entry name" value="Snurportin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21974">
        <property type="entry name" value="SPN1_m3Gcap_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037955">
        <property type="entry name" value="Snurportin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="14">
        <location>
            <begin position="11"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="67"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="331"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with m3G-cap structure" evidence="11">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with m3G-cap structure" evidence="11">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with m3G-cap structure" evidence="11">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037955-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="382" mass="43321" checksum="166393B490BFAAF4" modified="2020-06-17" version="1">MDDLTQALSASFAVSKDPNSTAAPHPRLAQYKSKYSVLEQSERRRRFLDLQKSKRLNYVNHARRLADGDWTGADSDGEDDMEKQEEGEQNQDGNKVAEEEEEGMEIERRKLPKHYANQLMLSEWLVDVPSELDTDWLMVVCPVGKRSLIVASKGSTAAYTKSGYCVNRFPSLLPGGNRHNSAMGKDYTILDCIYSEVDRTYYILDVMCWRGHPVYDCPTEFRFYWLQSKVQETDGLTEIAKRNPFRFVSLQSTDCTAESIQRALAAEYSFSVDGLLFYHRQTHYTPGSTPLVGWLRPYMVSDILGIEVPVGPLTNKPEYASHQLQQILEHKKTSSEVRPANRSGGYELEHLSTPSQDSGDGVNFLNQKPIRESLEVEQSAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AS98</accession>
    <name>A0A673AS98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Potassium inwardly rectifying channel subfamily J member 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">kcnj8</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030006468.1">
        <property type="nucleotide sequence ID" value="XM_030150608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AS98"/>
    <dbReference type="FunCoup" id="A0A673AS98">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032178.1">
        <property type="protein sequence ID" value="ENSSORP00005031303.1"/>
        <property type="gene ID" value="ENSSORG00005014902.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430534"/>
    <dbReference type="CTD" id="3764"/>
    <dbReference type="InParanoid" id="A0A673AS98"/>
    <dbReference type="OrthoDB" id="273257at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000050">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003278">
        <property type="entry name" value="K_chnl_inward-rec_Kir6.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF11">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01331">
        <property type="entry name" value="KIR61CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 14" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 14" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6 14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="17">
        <location>
            <begin position="37"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="18">
        <location>
            <begin position="182"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="364"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="13">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="46894" checksum="E8A34230E6FEA212" modified="2020-06-17" version="1">MLARKSIIPEEFGLPGLASRMPRKPVFRDRVNKARFIAKNGSCNLAHKNIREQGRFLQDVFTTLVDLKWRFTLVIFTTTFVSSWLFFAMNWWLVAFAHGDLDPGPQNRTHCVTDVKSFTSAFLFSIEVQVTIGFGGRMITEQCAAAITVLIMQNIVGLIINAVMLGCIFMKTAQSHRRAETLIFSRHAVIAVRNNRLCFMIRIGDLRKSMIIGATIRLQVVRKTTTPEGEVIPIHQIDVQTESAVASNSLFLLAPLIICHIIDKNSPLYDLSAMELQCSDLEVIAILEGVVETTGITTQARTSYVSEEIQWGHRFVPIVTEEEGVYSVDYSKFGNTVKVATPRCSARELDEKPSILIQTLQKSELSHQNSLRKRNSMRRNNSMRKGTGSSGGLRRNNSGLLAPKVQFFTEGGQNLNAVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AV22</accession>
    <name>A0A673AV22_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD repeat-containing protein 37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115411237</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AV22"/>
    <dbReference type="Ensembl" id="ENSSORT00005033105.1">
        <property type="protein sequence ID" value="ENSSORP00005032218.1"/>
        <property type="gene ID" value="ENSSORG00005015295.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AV22"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000080">
        <property type="entry name" value="WD repeat-containing protein 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000152">
        <property type="entry name" value="WD repeat-containing protein 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000666">
        <property type="entry name" value="WD repeat-containing protein 37 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
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        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="158"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
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            <begin position="321"/>
            <end position="362"/>
        </location>
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            <begin position="363"/>
            <end position="404"/>
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        <location>
            <begin position="407"/>
            <end position="447"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        </location>
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            <begin position="290"/>
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            <begin position="1"/>
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    </feature>
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            <begin position="16"/>
            <end position="25"/>
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        <location>
            <begin position="297"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022574"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00040954"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="554" mass="61112" checksum="16D300D4B2A5487A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYJ9</accession>
    <name>A0A673AYJ9_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">DUF1518 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSSORG00005016441.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="F:transcription coactivator activity"/>
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        <property type="term" value="P:cellular response to hormone stimulus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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    </dbReference>
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        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="37"/>
            <end position="99"/>
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            <end position="79"/>
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            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="SMART" id="SM01151"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="264" mass="28042" checksum="C47B7C40A26B3EBE" modified="2020-06-17" version="1">MLAQRQRELYSIQHRQRQLFQQKVMLMRQNMAAAPAGAVGSIGTARQQQQQQQQFNFPPGYSPMTGNPPTSPSHFSPMSTGPLDNKLSARVPLNNQTLMGGVQGQFNSNVNASLQQGLFQQFGGSAIVQQDPSFPPEMSPTSPLLSPQNSTSQSPLLQQVPPPGYQSPDMKSWQQTGMGSNSLFSQSGQSAGQAFGQQGVYNNMSITVSMAGGSGGVASIPPIGQPVGMSNSNLSNVGSVCSDQQVRDGLGGFCLSISLVLQEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYN8</accession>
    <name>A0A673AYN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosinase</fullName>
            <ecNumber evidence="4">1.14.18.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Monophenol monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115432423</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Melanosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tyrosinase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.18.1" evidence="4"/>
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        <property type="protein sequence ID" value="ENSSORP00005033418.1"/>
        <property type="gene ID" value="ENSSORG00005015148.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:melanosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004503">
        <property type="term" value="F:tyrosinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042438">
        <property type="term" value="P:melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043473">
        <property type="term" value="P:pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1280.10:FF:000003">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF124">
        <property type="entry name" value="TYROSINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00497">
        <property type="entry name" value="TYROSINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0186">Copper</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0470">Melanin biosynthesis</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025426182" description="Tyrosinase" evidence="18">
        <location>
            <begin position="21"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="476"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="19">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="20">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="546" mass="62397" checksum="E7F79DE34F628E97" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1F9</accession>
    <name>A0A673B1F9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">5'-3' exoribonuclease</fullName>
            <ecNumber evidence="12">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">xrn2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_029984713.1">
        <property type="nucleotide sequence ID" value="XM_030128853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B1F9"/>
    <dbReference type="FunCoup" id="A0A673B1F9">
        <property type="interactions" value="2055"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036625.1">
        <property type="protein sequence ID" value="ENSSORP00005035670.1"/>
        <property type="gene ID" value="ENSSORG00005016846.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415325"/>
    <dbReference type="CTD" id="22803"/>
    <dbReference type="InParanoid" id="A0A673B1F9"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12390:FF:000003">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017151">
        <property type="entry name" value="Xrn2/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037239">
        <property type="entry name" value="Exonuclease_Xrn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="15">
        <location>
            <begin position="326"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="409"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="485"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="793"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="831"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="793"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="894"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="935"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="974" mass="111888" checksum="F6D7E134D1534086" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B350</accession>
    <name>A0A673B350_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytotoxic T-lymphocyte protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cd28</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B350"/>
    <dbReference type="Ensembl" id="ENSSORT00005036022.1">
        <property type="protein sequence ID" value="ENSSORP00005035092.1"/>
        <property type="gene ID" value="ENSSORG00005016553.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B350"/>
    <dbReference type="OrthoDB" id="9908091at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042129">
        <property type="term" value="P:regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040216">
        <property type="entry name" value="CTLA4/CD28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11494">
        <property type="entry name" value="CYTOTOXIC T-LYMPHOCYTE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11494:SF8">
        <property type="entry name" value="CYTOTOXIC T-LYMPHOCYTE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025558014" evidence="10">
        <location>
            <begin position="20"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="25367" checksum="499FC6AA9726DFCF" modified="2020-06-17" version="1">MFLTHCVIVLTVLIQPVWSAANVIQPYRVDSTNGTARVQCLIQDDTPSYPYSDPEELRVILLKGLHGNQELCSSILNITEKKEVDTKTKGHVQCTPEVKEGAVEVMVSGLKPTDTDIYRCEIQIFYPPPFLRLTGNGTLIHVLGSSDCSLAERQITHQNDDDDDDDGDGERKVPVSLPVAVLMTLVILVLIVIIYLQTVQCLRGRRETIRQAAIANMFHKVDTAGFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6A1</accession>
    <name>A0A673B6A1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DBB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pik3ap1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030012115.1">
        <property type="nucleotide sequence ID" value="XM_030156255.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A673B6A1">
        <property type="interactions" value="651"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037832.1">
        <property type="protein sequence ID" value="ENSSORP00005036864.1"/>
        <property type="gene ID" value="ENSSORG00005017344.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434347"/>
    <dbReference type="CTD" id="118788"/>
    <dbReference type="InParanoid" id="A0A673B6A1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036312">
        <property type="term" value="F:phosphatidylinositol 3-kinase regulatory subunit binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B-cell_PI3K-Signaling_Adptrs"/>
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    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16267">
        <property type="entry name" value="BANK1/PIK3AP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16267:SF12">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE ADAPTER PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="DBB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TIR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="219"/>
            <end position="355"/>
        </location>
    </feature>
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        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
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        <location>
            <begin position="608"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="752"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="576"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="754"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="809"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="820"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51376"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="861" mass="97274" checksum="D2CC02F3B3AC834B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BBI9</accession>
    <name>A0A673BBI9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BBI9"/>
    <dbReference type="Ensembl" id="ENSSORT00005040192.1">
        <property type="protein sequence ID" value="ENSSORP00005039185.1"/>
        <property type="gene ID" value="ENSSORG00005018317.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="CASIMO1_SMIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="71" mass="8106" checksum="D27CB751D263D59D" modified="2020-06-17" version="1">MADSNLQQEFEDQFNDVVSRLQSKQLFQSDWDIASFAIFFIFIAVNHICQSKPGTADVKASFCCKILLTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BBY1</accession>
    <name>A0A673BBY1_9TELE</name>
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        <submittedName>
            <fullName evidence="11">Spondin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">spon2a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BBY1"/>
    <dbReference type="Ensembl" id="ENSSORT00005038930.1">
        <property type="protein sequence ID" value="ENSSORP00005037943.1"/>
        <property type="gene ID" value="ENSSORG00005017792.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BBY1"/>
    <dbReference type="OrthoDB" id="6090599at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Spondin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-spondin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051418">
        <property type="entry name" value="Spondin/Thrombospondin_T1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009465">
        <property type="entry name" value="Spondin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038678">
        <property type="entry name" value="Spondin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044004">
        <property type="entry name" value="TSP1_spondin_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038123">
        <property type="entry name" value="NF038123_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11311:SF33">
        <property type="entry name" value="MINDIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11311">
        <property type="entry name" value="SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06468">
        <property type="entry name" value="Spond_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19028">
        <property type="entry name" value="TSP1_spondin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51020">
        <property type="entry name" value="SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025377564" evidence="9">
        <location>
            <begin position="28"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin" evidence="10">
        <location>
            <begin position="34"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="333" mass="36956" checksum="98A414EB9341E4A9" modified="2020-06-17" version="1">MMSSEHLTCGWLQQLLFVLMKLSLSFAGPLRPLNGTECTAKGPASYILVFTGHWSPQAFPKQYPLFRPPAQWSKLIAVSHNRHFRLWEEGASASAGLQSFAELGVTVEIMKGAKEARKRRAVGAMYRTAGIPNGIGHSSTELLMLPRSSLLSLMVKMIPSPDWFVGVDSLNLCDGNQWKEEVTVDLHPYDAGTDSGFTFSSPNFPTSPPENITKITSQMPNHPANSFYYPRLKELPPIASVKLTQQSRSLHRQTVMSNHILPNSISPQRFSATPLDCEVSLWSSWGLCLGPCSRGGVRHRTRYILLRPANSGNPCPELEEQAECVPHSCLKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCP8</accession>
    <name>A0A673BCP8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Laminin G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BCP8"/>
    <dbReference type="Ensembl" id="ENSSORT00005041293.1">
        <property type="protein sequence ID" value="ENSSORP00005040256.1"/>
        <property type="gene ID" value="ENSSORG00005018778.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BCP8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000003">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF57">
        <property type="entry name" value="NEUREXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025623706" description="Laminin G domain-containing protein" evidence="11">
        <location>
            <begin position="22"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="12">
        <location>
            <begin position="55"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="290"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="383" mass="41908" checksum="909CE663F0C4E719" modified="2020-06-17" version="1">SLLVWGSLLVWASAGLGPCWSGGPCWSGVVFLSVLCPPSTLILFSFLSFFLSAGTTYIFGKGGGLITFRWPANDRPSTRTDRLTVGFSTALKEGILVRIDSAPGLGDFLMLHIVQGKIGVTFNIGTVDISVQESSTPVNDGKYHVVRFTRNGGNATLQVDNWAINEHFPTGNSDMERYQMANKKIPFIYVRPVEEWLQEKGRKLTIFNTQATVAIGGSDRGRPFQGQLSGLYYNGLKVLNMAAEANANIKINGSVRLVGDVPSVGGSARTTAMSPDLSTSFIETTTMMSTTTTRKHRSSSTVQHATDDIVSSAECSSDDEDLEECETSNAGPSAEHHSPMGPQVYLQEKQACYWYWSWYCGGQQHLCKLRLCSHCRPYETKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCV2</accession>
    <name>A0A673BCV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal fusion of the chorion and the allantois during placenta development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BCV2"/>
    <dbReference type="FunCoup" id="A0A673BCV2">
        <property type="interactions" value="807"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039245.1">
        <property type="protein sequence ID" value="ENSSORP00005038253.1"/>
        <property type="gene ID" value="ENSSORG00005017902.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BCV2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013300">
        <property type="entry name" value="Wnt7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF73">
        <property type="entry name" value="PROTEIN WNT-7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01891">
        <property type="entry name" value="WNT7PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="347" mass="39022" checksum="A40449CC2863056E" modified="2020-06-17" version="1">CVCVSVWWLVCVTLCVHVCVIVALSSVVALGANIICNKIPGLAPRQRAICQSRPDAIIVVGEGAQMGINECQYQFRYGRWNCSALGERTVFGQELRVGSREAAFTYAITAAGVAHAITAACSQGNLSQCGCDREKQGYYNQEEGWKWGGCSADIKYGIEFSRRFVDAREIKKTPRRLMNLHNNEAGRKVLEERMKLECKCHGVSGSCTTKTCWTTLPKFREIGYVLKDKYNEAVHVEVVRASRLRQPTHLKVKQTQGYRKPMETDLVYIERSPNYCEEDAATGSVGTQGRLCNRTSPHTDGCDLMCCGRGYNTHQYTKVWQCNCKFQWCCFVKCNTCSERTEVFTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFS8</accession>
    <name>A0A673BFS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Multivesicular body subunit 12A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ESCRT-I complex subunit MVB12A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Protein FAM125A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">mvb12a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MVB12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989367.1">
        <property type="nucleotide sequence ID" value="XM_030133507.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029997018.1">
        <property type="nucleotide sequence ID" value="XM_030141158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BFS8"/>
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        <property type="interactions" value="766"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040210.1">
        <property type="protein sequence ID" value="ENSSORP00005039203.1"/>
        <property type="gene ID" value="ENSSORG00005018323.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115409991"/>
    <dbReference type="CTD" id="93343"/>
    <dbReference type="InParanoid" id="A0A673BFS8"/>
    <dbReference type="OrthoDB" id="6021306at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032510">
        <property type="term" value="P:endosome to lysosome transport via multivesicular body sorting pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:receptor catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042058">
        <property type="term" value="P:regulation of epidermal growth factor receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046755">
        <property type="term" value="P:viral budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:virus maturation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Multivesicular body subunit 12B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040335">
        <property type="entry name" value="MVB12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018798">
        <property type="entry name" value="MVB12A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023340">
        <property type="entry name" value="UMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31612">
        <property type="entry name" value="MULTIVESICULAR BODY SUBUNIT 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31612:SF2">
        <property type="entry name" value="MULTIVESICULAR BODY SUBUNIT 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10240">
        <property type="entry name" value="DUF2464"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51497">
        <property type="entry name" value="UMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MABP" evidence="16">
        <location>
            <begin position="9"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="UMA" evidence="15">
        <location>
            <begin position="218"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="286" mass="31201" checksum="77EB442EC85BDE0D" modified="2020-06-17" version="1">MSLMEHGGVRPLTALAWTSNSSTCPKDFTVISITEDGATANFTRSFGMKSGYYLCYSKDLSGGMVLSDIQLISDKDSIPHGYCYIAEYLEPRATVSKKKRVCVRVVPVGSVDTAVLDVKLTAKSKMMLQHYTYVGDIHGYVLWCRKGPFSGPMPQAKPRSVSLDLRKLSLEQSSGPLPLRPSNPPPLPPGRLSQRRCSLHTKDNDKPADGSMQGITALDGVPFSLHPKFDVQTNGTGSQLNSQLNNIRIKSLQDIENEYNYTFAVEENAAKRTRPSVPAGGASSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BHF8</accession>
    <name>A0A673BHF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Receptor (G protein-coupled) activity modifying protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ramp2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RAMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997703.1">
        <property type="nucleotide sequence ID" value="XM_030141843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BHF8"/>
    <dbReference type="Ensembl" id="ENSSORT00005041757.1">
        <property type="protein sequence ID" value="ENSSORP00005040704.1"/>
        <property type="gene ID" value="ENSSORG00005019003.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424520"/>
    <dbReference type="CTD" id="10266"/>
    <dbReference type="InParanoid" id="A0A673BHF8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.510">
        <property type="entry name" value="Receptor activity modifying family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006985">
        <property type="entry name" value="RAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038126">
        <property type="entry name" value="RAMP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14076:SF10">
        <property type="entry name" value="RAMP2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14076">
        <property type="entry name" value="RECEPTOR ACTIVITY MODIFYING PROTEIN RAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04901">
        <property type="entry name" value="RAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23226" checksum="DA446C649F11A974" modified="2020-06-17" version="1">MTGKMTTRHFSFLLSRCFISFLILVGHTTVLCLVDEKLAVHPTTTVAYHFTDMMNGTTNASAGEDILPFDCGNNSHNCKNFCHLCYEFYQGPTMDCLSGLLNLCLHTFDVEMAQNSTNWCIWGNVSGSYSNLSLCTEEISDCLLIPWPNPLVEQIFVDIHTKFFKDCPTEELSDPPPVIIFALVITPICLIPVMVSLVVLKTKNGDGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIK0</accession>
    <name>A0A673BIK0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115416351</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BIK0"/>
    <dbReference type="Ensembl" id="ENSSORT00005042125.1">
        <property type="protein sequence ID" value="ENSSORP00005041069.1"/>
        <property type="gene ID" value="ENSSORG00005019133.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BIK0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150105">
        <property type="term" value="P:protein localization to cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF2">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin tail" evidence="4">
        <location>
            <begin position="756"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="111"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="519"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="701"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1026" mass="117745" checksum="602E1A1309356707" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIK5</accession>
    <name>A0A673BIK5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">COBW domain containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cbwd</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BIK5"/>
    <dbReference type="FunCoup" id="A0A673BIK5">
        <property type="interactions" value="449"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043484.1">
        <property type="protein sequence ID" value="ENSSORP00005042406.1"/>
        <property type="gene ID" value="ENSSORG00005019658.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BIK5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="8">
        <location>
            <begin position="250"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="359" mass="40354" checksum="8573574F42EA0764" modified="2020-06-17" version="1">MEEEDECPELVPIDTQTSPPAEQIPVTIITGYLGAGKTTLLNYILTEQHNKRIAVILNEFGEGSALEKSLAVSQAGELYEEWLELRNGCLCCSVKDNGLKAIENLMEKKGKFDYILLETTGLADPGAVASMFWVDAELGSDIYLDGIVTVIDAKYGLQNLCFCIYCFYRQIALADLVIINKTDLVNEEELAQSRDTVRYVSQVKLSLIFSHRVDLSEVLDLHSFDSKNGANLAEKLQLMKTTRPHLDKSILTVTFEVAGDLSEDALNVFVQDLLWEKMFKNKEGQPMTVIRLKGIVSFAGKAHQVMLQGVHELYELNETPQLWEENPRINRLVFIGRSLDKDTLQEEFISRVVQGANKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJB5</accession>
    <name>A0A673BJB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Potassium voltage-gated channel subfamily KQT member 5 KQT-like 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BJB5"/>
    <dbReference type="Ensembl" id="ENSSORT00005042922.1">
        <property type="protein sequence ID" value="ENSSORP00005041850.1"/>
        <property type="gene ID" value="ENSSORG00005019432.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJB5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF8">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="16917" checksum="2CD7D6219F263897" modified="2020-06-17" version="1">MPRNHSGDEGSAGLWMRTSPGHRTEGYGLKDVESGRRMKMKNSAGGDGLLSASSTVTGAPGSESLRGKQGARLSLLGKPLIYSAQSGRRNVRYRRLQNYLYNVLERPRAWAFIYHAFVVKCKCPQSPHSTVDVDNFIPDTRYCSKLRVDQIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLX7</accession>
    <name>A0A673BLX7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="22">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">insrb</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="22"/>
    <dbReference type="Ensembl" id="ENSSORT00005043871.1">
        <property type="protein sequence ID" value="ENSSORP00005042785.1"/>
        <property type="gene ID" value="ENSSORG00005019259.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLX7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005899">
        <property type="term" value="C:insulin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005009">
        <property type="term" value="F:insulin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043560">
        <property type="term" value="F:insulin receptor substrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
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        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Receptor L-domain"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
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        <property type="entry name" value="Furin_repeat"/>
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    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Insulin_TMD"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR000494">
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        <property type="entry name" value="RTK"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Tyr_kinase_insulin-like_rcpt"/>
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        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
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        <property type="entry name" value="INSULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="FU"/>
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        <property type="entry name" value="TyrKc"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
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    <keyword evidence="9 20 21" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="16 22" id="KW-0675">Receptor</keyword>
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    <keyword evidence="7" id="KW-0732">Signal</keyword>
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    <keyword evidence="6 22 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 24" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="954"/>
            <end position="979"/>
        </location>
    </feature>
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        <location>
            <begin position="624"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="844"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="1018"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1324"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="19">
        <location>
            <position position="1155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1028"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20 21">
        <location>
            <position position="1052"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="1099"/>
            <end position="1105"/>
        </location>
        <ligand>
            <name>ATP</name>
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    <feature type="binding site" evidence="20">
        <location>
            <begin position="1159"/>
            <end position="1160"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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    <feature type="binding site" evidence="20">
        <location>
            <position position="1173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000620-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000620-2"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000312"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1378" mass="155232" checksum="A49B261AB80C861C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLX9</accession>
    <name>A0A673BLX9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Histone acetyltransferase</fullName>
            <ecNumber evidence="4 14">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">kat2a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
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            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4 14"/>
    <dbReference type="AlphaFoldDB" id="A0A673BLX9"/>
    <dbReference type="FunCoup" id="A0A673BLX9">
        <property type="interactions" value="1092"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042320.1">
        <property type="protein sequence ID" value="ENSSORP00005041258.1"/>
        <property type="gene ID" value="ENSSORG00005019211.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLX9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060349">
        <property type="term" value="P:bone morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060173">
        <property type="term" value="P:limb development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903010">
        <property type="term" value="P:regulation of bone development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061035">
        <property type="term" value="P:regulation of cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone acetyltransferase KAT2B"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
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    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
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        <property type="entry name" value="Bromodomain-like_sf"/>
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        <property type="entry name" value="Bromodomain_CS"/>
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    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016376">
        <property type="entry name" value="GCN5/PCAF"/>
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    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009464">
        <property type="entry name" value="PCAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF1">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone_acetylase_PCAF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
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        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
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    </dbReference>
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        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 17" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="20">
        <location>
            <begin position="468"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="19">
        <location>
            <begin position="710"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="15">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="544"/>
            <end position="546"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="551"/>
            <end position="557"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="582"/>
            <end position="585"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR003048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR003048-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR003048-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="802" mass="91388" checksum="5367F0C0AB795350" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMZ9</accession>
    <name>A0A673BMZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR2</fullName>
            <ecNumber evidence="4">3.1.3.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Myotubularin-related protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphatidylinositol-3-phosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">mtmr2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Lipid phosphatase that specifically dephosphorylates the D-3 position of phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate, generating phosphatidylinositol and phosphatidylinositol 5-phosphate. Regulates the level of these phosphoinositides critical for various biological processes including autophagy initiation and autophagosome maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1-D-myo-inositol-3-phosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42328"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65221"/>
            <dbReference type="ChEBI" id="CHEBI:78934"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:42329"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:45632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78911"/>
            <dbReference type="ChEBI" id="CHEBI:85342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:45633"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:39019"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="EC" id="3.1.3.95"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:39020"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:12317"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.95" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030010047.1">
        <property type="nucleotide sequence ID" value="XM_030154187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BMZ9"/>
    <dbReference type="FunCoup" id="A0A673BMZ9">
        <property type="interactions" value="1070"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044244.1">
        <property type="protein sequence ID" value="ENSSORP00005043150.1"/>
        <property type="gene ID" value="ENSSORG00005019294.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433016"/>
    <dbReference type="CTD" id="8898"/>
    <dbReference type="InParanoid" id="A0A673BMZ9"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14590">
        <property type="entry name" value="PTP-MTMR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000038">
        <property type="entry name" value="Myotubularin 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF42">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="23">
        <location>
            <begin position="206"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="387"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="619"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="21"/>
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        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025716388" evidence="21">
        <location>
            <begin position="26"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="530"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="23">
        <location>
            <begin position="34"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="24">
        <location>
            <begin position="247"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="423"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="438"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="556" mass="63557" checksum="ED882EA8CCD86747" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSS0</accession>
    <name>A0A673BSS0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cyclin-dependent kinase 14</fullName>
            <ecNumber evidence="5">2.7.11.22</ecNumber>
            <ecNumber evidence="4">2.7.11.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cell division protein kinase 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">cdk14</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="5"/>
    <dbReference type="EC" id="2.7.11.24" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673BSS0"/>
    <dbReference type="Ensembl" id="ENSSORT00005045345.1">
        <property type="protein sequence ID" value="ENSSORP00005044224.1"/>
        <property type="gene ID" value="ENSSORG00005020390.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BSS0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF154">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 23" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="89"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="323" mass="36183" checksum="CD7A1BA73CDE63A0" modified="2020-06-17" version="1">MSTRSVQGSDSVIKPLDTIPEDKKVRVQRTQSGFDPFEKPASQVKRVHSENNACINAKSSSAGKESPKLRRHSSPSSPTSPKFGKADSYEKLEKLGEGSYATVYKGKSKVNGKLVALKVIRLQEEEGTPFTAIREASLLKGLKHANIVLLHDIIHTKETLTLVFEYVHTDLCQYMDKHPGGLHPDNVKLFLFQLLRGLSYIHQRYILHRDLKPQNLLISDTGELKLADFGLARAKSVPSHTYSNEVVTLWYRPPDVLLGSTDYSTCLDMWGVGCIFIEMIQGVAAFPGMKDIQDQLERIFLRDRSQESDVESMLSSSFVFTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVE4</accession>
    <name>A0A673BVE4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">F-box protein 40, tandem duplicate 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115433265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BVE4"/>
    <dbReference type="Ensembl" id="ENSSORT00005046230.1">
        <property type="protein sequence ID" value="ENSSORP00005045094.1"/>
        <property type="gene ID" value="ENSSORG00005020712.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVE4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.150">
        <property type="entry name" value="TRAF-like zinc-finger, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031890">
        <property type="entry name" value="Fbxo30/Fbxo40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
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    <dbReference type="InterPro" id="IPR043013">
        <property type="entry name" value="Znf_TRAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15933:SF21">
        <property type="entry name" value="F-BOX ONLY PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15933">
        <property type="entry name" value="PROTEIN CBG16327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15966">
        <property type="entry name" value="F-box_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15965">
        <property type="entry name" value="zf-TRAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FBOX"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="7">
        <location>
            <begin position="55"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="527"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="5">
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            <begin position="55"/>
            <end position="97"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="160"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="665" mass="75088" checksum="BD5834CA805A684B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4E3</accession>
    <name>A0A673C4E3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ythdf2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the YTHDF family. YTHDF2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029982450.1">
        <property type="nucleotide sequence ID" value="XM_030126590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C4E3"/>
    <dbReference type="SMR" id="A0A673C4E3"/>
    <dbReference type="FunCoup" id="A0A673C4E3">
        <property type="interactions" value="1596"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049308.1">
        <property type="protein sequence ID" value="ENSSORP00005048119.1"/>
        <property type="gene ID" value="ENSSORG00005021972.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413608"/>
    <dbReference type="CTD" id="51441"/>
    <dbReference type="InParanoid" id="A0A673C4E3"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000001">
        <property type="entry name" value="YTH domain family 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF8">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="11">
        <location>
            <begin position="446"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="207"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="607"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="637" mass="68244" checksum="C3E180F5DC7F8771" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5J0</accession>
    <name>A0A673C5J0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Claudin-8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cldn8.1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C5J0"/>
    <dbReference type="Ensembl" id="ENSSORT00005051906.1">
        <property type="protein sequence ID" value="ENSSORP00005050691.1"/>
        <property type="gene ID" value="ENSSORG00005022971.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C5J0"/>
    <dbReference type="OrthoDB" id="8819159at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025584547" evidence="12">
        <location>
            <begin position="22"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="260" mass="28736" checksum="FA09420C497DE227" modified="2020-06-17" version="1">MANSALEILGLLLTLIGLIGAAASTGMPMWRVTAFIGENIIVFETRYEGLWMNCFRQADIRMQCKVYDSLLALPPDLQAARGLMCCALALGGLGLLISLVGMQCTSCIQNNDRAKRMVLIIAGSMIIMACICVLIPVSWTGHVIIRDFYNPLLIDAQRRELGEALYIGWVASAFLMAGGCMFTCCNIQSDGKGSERYIYSRNSDYIAYPPQPLQPQQLVMLPQPQPQYYPQAQPVLSRHPSTVHSYHSRHPSVRSGVAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6A4</accession>
    <name>A0A673C6A4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cyclin D3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ccnd3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for the control of the cell cycle at the G2/M (mitosis) transition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C6A4"/>
    <dbReference type="Ensembl" id="ENSSORT00005049981.1">
        <property type="protein sequence ID" value="ENSSORP00005048789.1"/>
        <property type="gene ID" value="ENSSORG00005022209.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C6A4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000003">
        <property type="entry name" value="G1/S-specific cyclin-D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="3">
        <location>
            <begin position="70"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="253" mass="28248" checksum="62E06055C9FF3C07" modified="2020-06-17" version="1">MDSGSGEQLQNASIYKNKEVVVRAGSDPVLTRDHRALYHLKSLEKTGQVSPYFGGVQTDIQPDMRKILTVWMFQVCEEQKCEEEVFPLSVHYLDCYLSRLYVERSNLQLLGSVCMFLASKMRETVPLTASKLCIYTDNSVSVSDILRWEVSVVSCLDWCLASVVPSDFLEPVLLSLSSIEPHHLPNIRKLVHSYIALAAIGKFLEFLPSTIACASVSLAVQKLKLLDVVSSSESVVTFLSNLLATNLVRIIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7A3</accession>
    <name>A0A673C7A3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inositol-tetrakisphosphate 1-kinase</fullName>
            <ecNumber evidence="4">2.7.1.134</ecNumber>
            <ecNumber evidence="3">2.7.1.159</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Inositol 1,3,4-trisphosphate 5/6-kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">itpk1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1D-myo-inositol 1,3,4-trisphosphate + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = 1D-myo-inositol 3,4,5,6-tetrakisphosphate + 1D-myo-inositol 1,3,4,5-tetrakisphosphate</text>
            <dbReference type="Rhea" id="RHEA:70271"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:70272"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:70273"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1D-myo-inositol 1,3,4-trisphosphate + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = 1D-myo-inositol 3,4,5,6-tetrakisphosphate + 1D-myo-inositol 1,3,4,6-tetrakisphosphate</text>
            <dbReference type="Rhea" id="RHEA:70263"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:70264"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:70265"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1D-myo-inositol 1,3,4-trisphosphate + ATP = 1D-myo-inositol 1,3,4,5-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13253"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.159"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13254"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:13255"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1D-myo-inositol 1,3,4-trisphosphate + ATP = 1D-myo-inositol 1,3,4,6-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20940"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.159"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:20941"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:20942"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.134"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:12453"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:12454"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1D-myo-inositol 3,4,6-trisphosphate + ATP = 1D-myo-inositol 1,3,4,6-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:189099"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70288"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:70289"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITPK1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.134" evidence="4"/>
    <dbReference type="EC" id="2.7.1.159" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673C7A3"/>
    <dbReference type="FunCoup" id="A0A673C7A3">
        <property type="interactions" value="290"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049904.1">
        <property type="protein sequence ID" value="ENSSORP00005048712.1"/>
        <property type="gene ID" value="ENSSORG00005022188.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C7A3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.220:FF:000001">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11370:FF:000001">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000047">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041429">
        <property type="entry name" value="ITPK1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF1">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17927">
        <property type="entry name" value="Ins134_P3_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Inositol-tetrakisphosphate 1-kinase N-terminal" evidence="22">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="21">
        <location>
            <begin position="120"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF05770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF17927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="409" mass="45090" checksum="9CA721B02C8289A8" modified="2020-06-17" version="1">MQTFLKGRRVGYWLSEKKMKKLNFQAFADLCRKRGIEVVQLDLSQPLEDQGPLDVIIHKLTDLILEADQNDSQAVLLVQRVQDYIDAHPKTIVLDPLPAIRTLLDRCKSYQLVHRIESCMQDERICSPPFMVLNTECSPDVLEQIKKQGLTFPFSESTLKFDICCMCSCPQMAIIFSEEDLKDVKPPCVIQSFINHNAVLYKVFVVGDSYTVVERPSLKNFPAGPADRKAIFFNSHNVSKPESSSDLTSRDNVEGVSQPPSDDVIRELSRSLRQALGVSLFGIDVIINNQTGQHAVIDINAFPGYEGVPEFFNDLLNHISSVLQSHTPDFAPASEQPKGLPVSTTVPNTAAPAPGCCSMLGKEAGGSPWIVEGDGGLKGPRQRLGCNSAMSPNFQQHCVSTIATKASSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7R4</accession>
    <name>A0A673C7R4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytochrome c oxidase subunit 8B, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115420687</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase VIII family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029991990.1">
        <property type="nucleotide sequence ID" value="XM_030136130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C7R4"/>
    <dbReference type="FunCoup" id="A0A673C7R4">
        <property type="interactions" value="641"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050049.1">
        <property type="protein sequence ID" value="ENSSORP00005048857.1"/>
        <property type="gene ID" value="ENSSORG00005022234.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420687"/>
    <dbReference type="CTD" id="12869"/>
    <dbReference type="InParanoid" id="A0A673C7R4"/>
    <dbReference type="OrthoDB" id="8931496at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00930">
        <property type="entry name" value="Cyt_c_Oxidase_VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.81.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 8B, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.81.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003205">
        <property type="entry name" value="Cyt_c_oxidase_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036548">
        <property type="entry name" value="Cyt_c_oxidase_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16717">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16717:SF5">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 8, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02285">
        <property type="entry name" value="COX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81431">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIIb (aka IX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="68" mass="7483" checksum="3E7D910CA6EE6E0C" modified="2020-06-17" version="1">MSLRTAAYGLFRTALRTQVVQVARVSSKPAKHNVTAGEQAIAMTALFVAILVPSGWILAHLEDYKKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C990</accession>
    <name>A0A673C990_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">B2 bradykinin receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115414258</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029983391.1">
        <property type="nucleotide sequence ID" value="XM_030127531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C990"/>
    <dbReference type="Ensembl" id="ENSSORT00005051027.1">
        <property type="protein sequence ID" value="ENSSORP00005049824.1"/>
        <property type="gene ID" value="ENSSORG00005022607.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414258"/>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="term" value="F:bradykinin receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:C-C chemokine binding"/>
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        <property type="term" value="F:C-C chemokine receptor activity"/>
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        <property type="term" value="P:calcium-mediated signaling"/>
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        <property type="term" value="P:cell chemotaxis"/>
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        <property type="term" value="P:immune response"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Brdyknn_rcpt"/>
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        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF957">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRADYKININR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="54"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="346" mass="39065" checksum="71FC95EB7E3ED372" modified="2020-06-17" version="1">MALYHSFPVNITNTTINEDLVNTSNQCPETGTDQMIYTVVPVYIIIISVLGIVLNILVLLVFWLHKNPCTVPEIYLSNLAAADLLLMCFLPFWAVTIANKYNWFFGLHMCRMVNLCIVMNANRSIYFLVLVSIDRFLALVHPLSHASMRSRKCAKLGCVLVWCFGLILGIPTLICRKVKDDPGSNSSGCSFGCSETVIYTNEVVLTVLGFIIPVAIISYCTIKIIQALKNRPVEGVKIQKMEHRATFLVLVVLGAFLTCWGPFHIMRMIEIFLRTGIIPGNSNCEAVRVFDIFMHTFNYFAYFNSVLNPILYVIVAKDFRKKMCEVFKRCTKVRTSVLSVTTRLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9D5</accession>
    <name>A0A673C9D5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cyclin-dependent kinase inhibitor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cdkn1a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C9D5"/>
    <dbReference type="Ensembl" id="ENSSORT00005049975.1">
        <property type="protein sequence ID" value="ENSSORP00005048783.1"/>
        <property type="gene ID" value="ENSSORG00005022204.1"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:DNA damage response"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072331">
        <property type="term" value="P:signal transduction by p53 class mediator"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CDI_dom"/>
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        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029841">
        <property type="entry name" value="CDKN1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46778:SF1">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46778">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="4">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="103"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="163" mass="18431" checksum="E9FDBEBF1E920A52" modified="2020-06-17" version="1">MATHKRILSSLGNGPTRRNLFGPVDREQLTVEYQAALRKDLEEASRRWGFDFISDKPLESSDFQWEGIPGTKVPLLYRSCMFGVGPAQGRSEVVAAVYPKAGRVGPSESEKENILRTPERCPHNLEKTPEKRENTGLKRKQTNITDFYQAKRRVVGTPRKSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBD6</accession>
    <name>A0A673CBD6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">ADAM metallopeptidase with thrombospondin type 1 motif, 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">adamts6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBD6"/>
    <dbReference type="Ensembl" id="ENSSORT00005051829.1">
        <property type="protein sequence ID" value="ENSSORP00005050614.1"/>
        <property type="gene ID" value="ENSSORG00005022940.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBD6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000002">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000079">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF27">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
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    </dbReference>
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        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9 14 16" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025616731" evidence="18">
        <location>
            <begin position="21"/>
            <end position="1112"/>
        </location>
    </feature>
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            <begin position="258"/>
            <end position="463"/>
        </location>
    </feature>
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        <location>
            <begin position="1074"/>
            <end position="1112"/>
        </location>
    </feature>
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        <location>
            <begin position="227"/>
            <end position="259"/>
        </location>
    </feature>
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            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
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        <location>
            <position position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="266"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="346"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="14 16">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 16">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="458"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <location>
            <begin position="321"/>
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        </location>
    </feature>
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    <feature type="disulfide bond" evidence="15">
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    <feature type="disulfide bond" evidence="15">
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            <begin position="485"/>
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    <feature type="disulfide bond" evidence="15">
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    <feature type="disulfide bond" evidence="15">
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    <feature type="disulfide bond" evidence="15">
        <location>
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    <feature type="disulfide bond" evidence="15">
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    <feature type="disulfide bond" evidence="15">
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    <feature type="disulfide bond" evidence="15">
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            <begin position="580"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004498"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1112" mass="124083" checksum="6D18D32032805596" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCM8</accession>
    <name>A0A673CCM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein YIPF3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">YIP1 family member 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">yipf3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in the maintenance of the Golgi structure. May play a role in hematopoiesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030012059.1">
        <property type="nucleotide sequence ID" value="XM_030156199.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005053331.1"/>
        <property type="gene ID" value="ENSSORG00005023976.1"/>
    </dbReference>
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    <dbReference type="CTD" id="25844"/>
    <dbReference type="InParanoid" id="A0A673CCM8"/>
    <dbReference type="OrthoDB" id="10256463at2759"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051521">
        <property type="entry name" value="tRNA_Mod/Golgi_Maint"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15627">
        <property type="entry name" value="NATURAL KILLER CELL-SPECIFIC ANTIGEN KLIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15627:SF14">
        <property type="entry name" value="PROTEIN YIPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
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            <begin position="181"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
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            <begin position="216"/>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="339" mass="37648" checksum="F0DA1B76477999BF" modified="2020-06-17" version="1">MSGGSGTRNTNTEPWGSFDDHLIQGSGAAVIDMENMDDTSGSSFEDMGEMHQRMKEEEEVTAEAAATDDDTHDDGEFLGMKGLKGQLGRQVADEVWQAGKRQASRAFNLYANIDILRPYFDVEPVQVRSRLIESLIPVRMINFPQKIAGELYGPLMLVFTLVAILLHGMKTSDTVIREGTLMGTAIGTCFGYWLGVSSFIYFLAYLVNAQITMLQMLSLLGYGLFGHCVVLLITYNIHFHFLFYVLWLLIGGLSTLRMVAALLSRTVGQTPRLLLCGTLSLLHMLFLLYLHFNYHKIVEGLLDSLDGPHMAPMQRVARDVAELTLNASVRSLGVTLRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CIY4</accession>
    <name>A0A673CIY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-binding cassette sub-family F member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">abcf2b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CIY4"/>
    <dbReference type="FunCoup" id="A0A673CIY4">
        <property type="interactions" value="1430"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053865.1">
        <property type="protein sequence ID" value="ENSSORP00005052617.1"/>
        <property type="gene ID" value="ENSSORG00005023451.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CIY4"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000467">
        <property type="entry name" value="ATP-binding cassette sub-family F member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF15">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="81"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="391"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="157"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="611" mass="69978" checksum="2B4413B03C9027D2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKE2</accession>
    <name>A0A673CKE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">wdr43</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029983058.1">
        <property type="nucleotide sequence ID" value="XM_030127198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CKE2"/>
    <dbReference type="Ensembl" id="ENSSORT00005053786.1">
        <property type="protein sequence ID" value="ENSSORP00005052538.1"/>
        <property type="gene ID" value="ENSSORG00005023506.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414016"/>
    <dbReference type="CTD" id="23160"/>
    <dbReference type="InParanoid" id="A0A673CKE2"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="115"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="6">
        <location>
            <begin position="460"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="586"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="404"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="586"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="632"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="642"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="654" mass="72000" checksum="A6390242E7375D12" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLR3</accession>
    <name>A0A673CLR3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AF4/FMR2 family, member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AF4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CLR3"/>
    <dbReference type="FunCoup" id="A0A673CLR3">
        <property type="interactions" value="1212"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055527.1">
        <property type="protein sequence ID" value="ENSSORP00005054254.1"/>
        <property type="gene ID" value="ENSSORG00005024322.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLR3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002151">
        <property type="term" value="F:G-quadruplex RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007797">
        <property type="entry name" value="AF4/FMR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043640">
        <property type="entry name" value="AF4/FMR2_CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043639">
        <property type="entry name" value="AF4_int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528">
        <property type="entry name" value="AF4/FMR2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528:SF18">
        <property type="entry name" value="AF4_FMR2 FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05110">
        <property type="entry name" value="AF-4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18875">
        <property type="entry name" value="AF4_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18876">
        <property type="entry name" value="AFF4_CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AF4/FMR2 C-terminal homology" evidence="6">
        <location>
            <begin position="513"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="310"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="353"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="642"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="153"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="323"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="417"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="480"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="642"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="766" mass="83523" checksum="6F76EB7E8FC4B417" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CPZ9</accession>
    <name>A0A673CPZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115419313</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CPZ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005059030.1">
        <property type="protein sequence ID" value="ENSSORP00005057711.1"/>
        <property type="gene ID" value="ENSSORG00005025556.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CPZ9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19721">
        <property type="entry name" value="bHLH_TS_NeuroD4_ATOH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000006">
        <property type="entry name" value="Neurogenic differentiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022575">
        <property type="entry name" value="NeuroD_DUF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF86">
        <property type="entry name" value="NEUROGENIC DIFFERENTIATION FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12533">
        <property type="entry name" value="Neuro_bHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="93"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
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            <begin position="241"/>
            <end position="331"/>
        </location>
    </feature>
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        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
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            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="331" mass="36588" checksum="3AD12E9BBDD05EB3" modified="2020-06-17" version="1">MIKPYMRRGEEEEAGSPLQWMDGDMSSPDGDASISSNRYRGSAVNQQPREIGSEDAEEDEEDEEEGQEDENGSKRRGPKKKRMTKARQERFRARRVKANARERSRMHGLNDALENLRSIMPCHSKTQKLSKIETLRLARNYICALSEALEGGLSTESRGFMETLCKGLSQPTTNLVAGCLQLGPSPAQGKPEDRHGVHVPPTPLGGVSYSSPGLPSPPYGTFDSAHLLHLRAMKGGLYDNHSPSEYNTGGVGTPPYDGPPTPPLSISSNLVPKQEPSPHYLPPHHYSPSSVDQNQGLYQTHSGYDPHPEGPYNSYHPPHMPPRQITSIYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CXC0</accession>
    <name>A0A673CXC0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CXC0"/>
    <dbReference type="FunCoup" id="A0A673CXC0">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059612.1">
        <property type="protein sequence ID" value="ENSSORP00005058289.1"/>
        <property type="gene ID" value="ENSSORG00005025740.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CXC0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025456136" description="C-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="21"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="125"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="239" mass="27729" checksum="F3323959561C06E4" modified="2020-06-17" version="1">MARPIFIIILCTVFDEAVGKHIYIPQKMKWLDAQAYCRSRYIDLSFVSSQSDQDQIEEASGTTPEKWIGLYLDPNDKVWKWSGGGVMTYENWANNQPDNGRNGTETKALIWPDGKWNDANEDSVLPFYCFTIDIDLVKMSWEGALEYCKKTNTSLISLNSEPEHLLIQTVVQNHRSDRVWIGLRYLGARWLWVNGEPLEYQAWPGQDHQCPAMNRCGALTKDRLWETRDCLEENHFICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y315</accession>
    <name>A0A672Y315_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sarcalumenin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">wu:fc22b06</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <property type="protein sequence ID" value="ENSSORP00005000068.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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            <begin position="20"/>
            <end position="978"/>
        </location>
    </feature>
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        <location>
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            <end position="821"/>
        </location>
    </feature>
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        </location>
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        </location>
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            <end position="117"/>
        </location>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000472271"/>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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</entry>
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        </lineage>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y4B6"/>
    <dbReference type="Ensembl" id="ENSSORT00005001176.1">
        <property type="protein sequence ID" value="ENSSORP00005001142.1"/>
        <property type="gene ID" value="ENSSORG00005000688.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4B6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="6">
        <location>
            <begin position="21"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="260" mass="29629" checksum="8D2F6F2AC9F908AE" modified="2020-06-17" version="1">MQFVLEYFLRFQDNLPGTYYISPSFRGEDPDSTHLNQFYHVECELLGDMDNAISIAEGYLAHLTQSMLKKHSDIILNTAGTLAHATAMLNKLEGKTPLPRISLDEAIPMMSSPDCLEWVQDGQPQFGRKLTRKGERLLIEKYGGAVWLTEMDHLGVPFYQAYVEGTGRCKAKAADLLLGLGETVGLGERHSTPELVQEALRHHAVPEQSYKWYINMRQVKTLLTSGWGMGTERYLCWLLQHDDIRDMHIIPRMKGMKYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4P2</accession>
    <name>A0A672Y4P2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">OCEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ell2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029990270.1">
        <property type="nucleotide sequence ID" value="XM_030134410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y4P2"/>
    <dbReference type="FunCoup" id="A0A672Y4P2">
        <property type="interactions" value="1135"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001359.1">
        <property type="protein sequence ID" value="ENSSORP00005001322.1"/>
        <property type="gene ID" value="ENSSORG00005000776.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419551"/>
    <dbReference type="CTD" id="22936"/>
    <dbReference type="InParanoid" id="A0A672Y4P2"/>
    <dbReference type="OrthoDB" id="6284217at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF8">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="8">
        <location>
            <begin position="552"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="292"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="516"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="666" mass="74686" checksum="50BA2D60F122CF2F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6V9</accession>
    <name>A0A672Y6V9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fbxl9</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y6V9"/>
    <dbReference type="FunCoup" id="A0A672Y6V9">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002160.1">
        <property type="protein sequence ID" value="ENSSORP00005002103.1"/>
        <property type="gene ID" value="ENSSORG00005001280.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y6V9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000103">
        <property type="entry name" value="Leucine rich repeat containing 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000647">
        <property type="entry name" value="Leucine-rich repeat-containing 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001330">
        <property type="entry name" value="Leucine-rich repeat-containing 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF169">
        <property type="entry name" value="F-BOX AND LEUCINE-RICH REPEAT PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="9"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="656" mass="72770" checksum="BD2677C7D7A7093B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y838</accession>
    <name>A0A672Y838_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyridoxine kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y838"/>
    <dbReference type="Ensembl" id="ENSSORT00005002871.1">
        <property type="protein sequence ID" value="ENSSORP00005002788.1"/>
        <property type="gene ID" value="ENSSORG00005001726.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y838"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="67" mass="7292" checksum="453FD8C98852EF35" modified="2020-06-17" version="1">MECRVLSIQSHVVRGYVGNKSATFPLQVKSGAYSVDSINSVQFSNHTGQLSNVCLHFAPPEVVGWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8U3</accession>
    <name>A0A672Y8U3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Baculoviral IAP repeat-containing protein 5.1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">birc5b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y8U3"/>
    <dbReference type="Ensembl" id="ENSSORT00005003295.1">
        <property type="protein sequence ID" value="ENSSORP00005003198.1"/>
        <property type="gene ID" value="ENSSORG00005001959.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8U3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000009">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF2">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 5.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="161" mass="18572" checksum="385F30D5DDEA68FC" modified="2020-06-17" version="1">HPPCNEPSGQFISFDKMYCHEMRHQTFADWPFREDSNCTPEKMVKAGFVHSPSENAPDVACCFFCLIELEGWEPDDDPWSEHAKRSPTCGFLTMKKDFTELTVAEYYNLEKDRLKVYIVSVHAVCTHSELCHTAIPITLKFFLRSRDSSMAAKRDEVELSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFW7</accession>
    <name>A0A672YFW7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672YFW7"/>
    <dbReference type="Ensembl" id="ENSSORT00005003560.1">
        <property type="protein sequence ID" value="ENSSORP00005003460.1"/>
        <property type="gene ID" value="ENSSORG00005002094.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFW7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF68">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="233" mass="26711" checksum="1E605EA18C46E3A1" modified="2020-06-17" version="1">MKRTDNLALREYDFERRFDERRALEWMQENWSKSFMFCGLYAALLNLRRPLVLWSLSLAIFSIIGAFRTDCTCYTSSQPAASDSPVCDISFYSAPVSKFWAYAFVLSKAPELGDTVFIVLRKQHQVAGGGWFMTMNYVVHSFMYTYYAARAAGLRVPRPCAMIITATQIMQMVMGLAVLGLVYHWMHEVRCPSYMDNIAWGSLMYLSYLILFALFFYNSYLKGSSGDKGSKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGB0</accession>
    <name>A0A672YGB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YGB0"/>
    <dbReference type="Ensembl" id="ENSSORT00005003700.1">
        <property type="protein sequence ID" value="ENSSORP00005003598.1"/>
        <property type="gene ID" value="ENSSORG00005002159.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003705.1">
        <property type="protein sequence ID" value="ENSSORP00005003603.1"/>
        <property type="gene ID" value="ENSSORG00005002159.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="178" mass="20211" checksum="40862EC52765F835" modified="2020-06-17" version="1">SWFFRSEVWVLAGSSAGLRFRSWVSAGSSAGLRFRFWVSAGSSGLRFRFWVSAGSSGLRFRSWVSAGSSGLRFRFWVSAGSSGLRFRFWVSAGSSGLRFRFWVSAGLRFRFWVSAGSSGLRFRFWVSAGSSGLRFRFWVSAGSSGLRFRFWVSAGRRNEQTCLFFRYNQKIQEICAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YH45</accession>
    <name>A0A672YH45_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115426589</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YH45"/>
    <dbReference type="Ensembl" id="ENSSORT00005003981.1">
        <property type="protein sequence ID" value="ENSSORP00005003870.1"/>
        <property type="gene ID" value="ENSSORG00005002346.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YH45"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025654154" evidence="3">
        <location>
            <begin position="20"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="660" mass="75874" checksum="2A05A1C6F5B188A0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHE2</accession>
    <name>A0A672YHE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115420048</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YHE2"/>
    <dbReference type="Ensembl" id="ENSSORT00005004077.1">
        <property type="protein sequence ID" value="ENSSORP00005003959.1"/>
        <property type="gene ID" value="ENSSORG00005002417.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YHE2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF29">
        <property type="entry name" value="COMPLEMENT C1Q SUBCOMPONENT SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025377140" description="C1q domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="7">
        <location>
            <begin position="109"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="239" mass="25979" checksum="D4B0003351EFD292" modified="2020-06-17" version="1">MHHHYLFWIGAIVSLVTPPLIAMETCPAAGIHGCIPGLPGRDGREGEKGQKGEPGIARHKAIGPQRGEKGEPGLMGFPGKRGQSGEPGEPGSSGISGPPGEPGEPGAVGIQQKAAFSVARGTNEFPDRGSIIRFTTVITNIDNDYNTDTGRFRCRVAGTYYFVYHASLEDRLCVLLKLDQNLLASFCDHRHHKRQVTSGGLAVYMSRNQEIWLETKDYRGMRGQHSGYSIFSGFLLHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ44</accession>
    <name>A0A672YJ44_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJ44"/>
    <dbReference type="Ensembl" id="ENSSORT00005004691.1">
        <property type="protein sequence ID" value="ENSSORP00005004557.1"/>
        <property type="gene ID" value="ENSSORG00005002766.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJ44"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003074">
        <property type="entry name" value="Immunoglobulin heavy variable 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025644556" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="38"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="124" mass="14118" checksum="7421AF725B9070DD" modified="2020-06-17" version="1">MADRMRTFLVFAVLVLTGVQGVDGQTLIESDSAVKRPGESHRLTCTTSGFTFSSYEMSWIRQIPGKGLEWIAYIRYDSGSIYYSQSVQGRFTISRENGRNQLYLQMNSLKTEDSAVYYCARETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLP0</accession>
    <name>A0A672YLP0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LHFPL tetraspan subfamily member 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLP0"/>
    <dbReference type="Ensembl" id="ENSSORT00005005596.1">
        <property type="protein sequence ID" value="ENSSORP00005005438.1"/>
        <property type="gene ID" value="ENSSORG00005003231.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLP0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060077">
        <property type="term" value="C:inhibitory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050811">
        <property type="term" value="F:GABA receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097112">
        <property type="term" value="P:gamma-aminobutyric acid receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019372">
        <property type="entry name" value="LHFPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489:SF14">
        <property type="entry name" value="LHFPL TETRASPAN SUBFAMILY MEMBER 4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489">
        <property type="entry name" value="LIPOMA HMGIC FUSION PARTNER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10242">
        <property type="entry name" value="L_HMGIC_fpl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="235" mass="25789" checksum="79CDCFF5AE5E7438" modified="2020-06-17" version="1">DPAEGGAAAARQKMLPSQEASKIYHDNYMRNSRAIGVLWAIFTICLAIITVVVFIQPYWIGDSVSTPQAGYFGLFHYCVGNGNANRELLCQGSFSDFRSIPSGAFKAASVFVLLSMVLILTCIGCFALFFFCNTATVYKTCAWMQLLCAVCLVLGCMIFPDGWDAEVVRDMCGEDTGKYTLGNCSVRWAYILAIIGILDALILSFLAFVLGNRQSDFLHEEIKNDNKGEGDDSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNN7</accession>
    <name>A0A672YNN7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ras-related protein Rab-1A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rab1ba</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YNN7"/>
    <dbReference type="FunCoup" id="A0A672YNN7">
        <property type="interactions" value="2010"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006368.1">
        <property type="protein sequence ID" value="ENSSORP00005006117.1"/>
        <property type="gene ID" value="ENSSORG00005003349.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YNN7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000069">
        <property type="entry name" value="Ras GTP-binding protein YPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="215" mass="23801" checksum="E0D34C95242B23D1" modified="2020-06-17" version="1">MAPTEGFGHFSSLSKNYLIDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLAIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGATAGGDKPNLKIESTPVRQSGGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRZ5</accession>
    <name>A0A672YRZ5_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YRZ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005007627.1">
        <property type="protein sequence ID" value="ENSSORP00005007349.1"/>
        <property type="gene ID" value="ENSSORG00005004204.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007629.1">
        <property type="protein sequence ID" value="ENSSORP00005007351.1"/>
        <property type="gene ID" value="ENSSORG00005004204.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
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        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627674" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="27"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="133" mass="14606" checksum="2E878B2D3F8B2132" modified="2020-06-17" version="1">MVLIQSMTLVSVLIWTLLCCCFTESRGQVTVTQPGAVSSDLGASTTITCSTSQNVYFTGSYHLLAWYQQRDGETPKLLIYLATRRQSGISSRFSGSGSNSHFTLTISDVQAEDAAVYYCQSAHVINSLWVFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YU31</accession>
    <name>A0A672YU31_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate receptor 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115433214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030010361.1">
        <property type="nucleotide sequence ID" value="XM_030154501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YU31"/>
    <dbReference type="Ensembl" id="ENSSORT00005008280.1">
        <property type="protein sequence ID" value="ENSSORP00005007997.1"/>
        <property type="gene ID" value="ENSSORG00005004478.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433214"/>
    <dbReference type="InParanoid" id="A0A672YU31"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025598978" evidence="1">
        <location>
            <begin position="23"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="96" mass="10576" checksum="9DD9868FCB01E1F9" modified="2020-06-17" version="1">MRISSGDWFVLIFSCFWGLTMGAFPSSVQIGGLFIRNTDQEYTAFRLAIFLHNTSPNASEAPFNLVPHVDNIETANSFAVTNACKSAAWVHAVLVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUC9</accession>
    <name>A0A672YUC9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Forkhead box O6 b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">foxo6b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YUC9"/>
    <dbReference type="FunCoup" id="A0A672YUC9">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008474.1">
        <property type="protein sequence ID" value="ENSSORP00005008188.1"/>
        <property type="gene ID" value="ENSSORG00005004556.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YUC9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001945">
        <property type="term" value="P:lymph vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20061">
        <property type="entry name" value="FH_FOXO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000032">
        <property type="entry name" value="Forkhead box protein O4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032067">
        <property type="entry name" value="FOXO-TAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032068">
        <property type="entry name" value="FOXO_KIX-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767:SF5">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16676">
        <property type="entry name" value="FOXO-TAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16675">
        <property type="entry name" value="FOXO_KIX_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="10">
        <location>
            <begin position="116"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="8">
        <location>
            <begin position="116"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="216"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="286"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="443"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="444"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="539"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="580"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="723" mass="78686" checksum="14BD2124ADA714C8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUH8</accession>
    <name>A0A672YUH8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cysteine dioxygenase</fullName>
            <ecNumber evidence="13">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cdo1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of cysteine to cysteine sulfinic acid with addition of molecular dioxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:20442"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="13"/>
    <dbReference type="RefSeq" id="XP_029999760.1">
        <property type="nucleotide sequence ID" value="XM_030143900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YUH8"/>
    <dbReference type="FunCoup" id="A0A672YUH8">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008476.1">
        <property type="protein sequence ID" value="ENSSORP00005008190.1"/>
        <property type="gene ID" value="ENSSORG00005004566.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425991"/>
    <dbReference type="CTD" id="1036"/>
    <dbReference type="InParanoid" id="A0A672YUH8"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="UniPathway" id="UPA00012">
        <property type="reaction ID" value="UER00537"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0042412">
        <property type="term" value="P:taurine biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
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        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
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        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="4 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0883">Thioether bond</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="11">
        <location>
            <begin position="93"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="201" mass="23407" checksum="65A247C32AF271BD" modified="2020-06-17" version="1">MEHTEVMVPETLDDLIKTLHKVFESDNVNVEEVQNIMESYVSKPHEWKKYGKFDQFRYTRNLVDEGNGKFNLIVLCWGEGHGSSIHDHTNSHCFMKMLQGQLKETLFEWPEAKSHGNMVQKSERILEENKVAYINDSIGLHRVENVSHTEGAVSLHLYSPPFQLCQTFDQRTGHKNTVKMTFWSKYGERTPFETTVSQENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUM9</accession>
    <name>A0A672YUM9_9TELE</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized LOC115416765</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115416765</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSSORP00005008298.1"/>
        <property type="gene ID" value="ENSSORG00005004614.1"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:MAP-kinase scaffold activity"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1536"/>
            <end position="1583"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="936"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="956"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="986"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1016"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1118"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1134"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1159"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1191"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1261"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1295"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1321"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1513"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1609"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1650"/>
            <end position="1661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1893" mass="205143" checksum="2802E910E88BED77" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYM3</accession>
    <name>A0A672YYM3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Retinal homeobox gene 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115430845</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a critical role in eye formation by regulating the initial specification of retinal cells and/or their subsequent proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YYM3"/>
    <dbReference type="FunCoup" id="A0A672YYM3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010021.1">
        <property type="protein sequence ID" value="ENSSORP00005009695.1"/>
        <property type="gene ID" value="ENSSORG00005005306.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYM3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048593">
        <property type="term" value="P:camera-type eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000071">
        <property type="entry name" value="Retinal homeobox gene 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043562">
        <property type="entry name" value="RAX/RAX2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46271">
        <property type="entry name" value="HOMEOBOX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46271:SF3">
        <property type="entry name" value="RETINAL HOMEOBOX PROTEIN RX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="104"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="14">
        <location>
            <begin position="284"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="106"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="304" mass="33664" checksum="01410BFFD1FD28E5" modified="2020-06-17" version="1">MWLLGSPSQVMMDERMSPSARSVQSPGNPSTVHSIEAILGFREDAIFHKSTSYNNMTDKMLGGDVERTGNVIVTPRRDKSLFSSVCPDSPDREGKLSDDENPKKKHRRNRTTFTTFQLHELERAFEKSHYPDVYSREELALKVNLPEVRVQVWFQNRRAKWRRQEKLEVSSIKLQDTSSCSPSSLLSFSRSPSRGSLGGGLQLDPWLSTPITTSTSLQSLPGFISGSQGLPAAYTPSPPPPPPPSMSSSLPASFLGHHSPHLPHIGSMCPPPVAYQCSADPRNSSIASLRMKAKEHIQSIGKSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1S1</accession>
    <name>A0A672Z1S1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">si:ch211-215k15.4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a defensive agent. Recognizes blood group fucosylated oligosaccharides including A, B, H and Lewis B-type antigens. Does not recognize Lewis A antigen and has low affinity for monovalent haptens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fucolectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z1S1"/>
    <dbReference type="Ensembl" id="ENSSORT00005011516.1">
        <property type="protein sequence ID" value="ENSSORP00005011141.1"/>
        <property type="gene ID" value="ENSSORG00005005710.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1S1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042806">
        <property type="term" value="F:fucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010185">
        <property type="term" value="P:regulation of cellular defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001868">
        <property type="term" value="P:regulation of complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051941">
        <property type="entry name" value="BG_Antigen-Binding_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713">
        <property type="entry name" value="FTP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713:SF8">
        <property type="entry name" value="SI:CH211-215K15.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="F5/8 type C" evidence="10">
        <location>
            <begin position="168"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="325" mass="35888" checksum="7A3D6544DAFC09D0" modified="2020-06-17" version="1">MHRDDDISWIHAKISYDTCEERDLLCLPANWLKNVALRGKATQSDRYEHMFGAAYNAIDGNRNSVFAADSCTHTDEQTNPWWRVDLLDSYIITSIKITNRGDCCPERLNGVQIHVGNSLEDNGLRNPIVGTIAAIAAGSTDTLSFTDHVEGRHVILVLPGINRFLTLCEVEVYGYRAPTGENLAVKGKASQSSLYPGGSPYNAIDGNRFSRWELGSCSHTAVENDPWWRLDLGKTHKVFSVKITNRFEVPERLIGAEILIGNSLENEGNNNTRCAVVTEIPGGATADFQCDGAHGMDGRYVNIIIPGRKECLTLCEVEVYGSVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1Z2</accession>
    <name>A0A672Z1Z2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">P2Y receptor family member 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z1Z2"/>
    <dbReference type="Ensembl" id="ENSSORT00005011066.1">
        <property type="protein sequence ID" value="ENSSORP00005010724.1"/>
        <property type="gene ID" value="ENSSORG00005005792.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1Z2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF6">
        <property type="entry name" value="PURINERGIC RECEPTOR P2Y, G-PROTEIN COUPLED 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="43"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="423" mass="48414" checksum="250189C0A99562EE" modified="2020-06-17" version="1">MNTSSVLVNSSTQCHHNMSGWEENMEKMYTYFYLVVFIPGLLLNTTALWVLCRHISKKTKAVIFMINLAFADLAHILSLPLRIYYYFTHTWPFGRTVCLFCFYLKYLNMYAAIVFLVCISMQRCVFLLDPFAARRWRRRYDLLISIIVWVVVGLACSPFILMRSNSNSSTSTSVSTHPVYNTSYFLEITSTQHPSAHTELYVTPLSTSPFTTTPTKAGCFKDLPMRRLPLSLAVTMMALAELFGFLIPLACISYSSFKIARSLMQSQTQKQDSITLSNSSTGRSRMQSVTSNCQTDKFHEKQTNGEKRRALRMVLSCSVLFLFCFAPYHLNFLLYLMVSQDILSHCAIRLAVRQFHPVSLCLASLSCCLNPLLYYFLTAEFRLHLTRRNSSFSSFLSSPVSSPTQRTVMHRLMSMESTSSDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z382</accession>
    <name>A0A672Z382_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z382"/>
    <dbReference type="Ensembl" id="ENSSORT00005011596.1">
        <property type="protein sequence ID" value="ENSSORP00005011218.1"/>
        <property type="gene ID" value="ENSSORG00005006029.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z382"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599:SF3">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="2">
        <location>
            <begin position="70"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="531" mass="61459" checksum="F9765F5F4360EDB9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4N6</accession>
    <name>A0A672Z4N6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metal transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">cnnm3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Metal transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ACDP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030005078.1">
        <property type="nucleotide sequence ID" value="XM_030149218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z4N6"/>
    <dbReference type="Ensembl" id="ENSSORT00005011807.1">
        <property type="protein sequence ID" value="ENSSORP00005011427.1"/>
        <property type="gene ID" value="ENSSORG00005006107.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429614"/>
    <dbReference type="CTD" id="26505"/>
    <dbReference type="InParanoid" id="A0A672Z4N6"/>
    <dbReference type="OrthoDB" id="5353557at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000001">
        <property type="entry name" value="Putative metal transporter CNNM3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057492">
        <property type="entry name" value="Ig_CNNM1/2/4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF27">
        <property type="entry name" value="METAL TRANSPORTER CNNM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25562">
        <property type="entry name" value="CNBH_CNNM2_C"/>
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</entry>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="544" mass="59283" checksum="6F80838FFD5F7BC3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBH3</accession>
    <name>A0A672ZBH3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphodiesterase</fullName>
            <ecNumber evidence="16">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">pde11al</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="16">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZBH3"/>
    <dbReference type="Ensembl" id="ENSSORT00005014616.1">
        <property type="protein sequence ID" value="ENSSORP00005014194.1"/>
        <property type="gene ID" value="ENSSORG00005007267.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZBH3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000003">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000004">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000018">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11" id="KW-0114">cAMP</keyword>
    <keyword evidence="7" id="KW-0140">cGMP</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDEase" evidence="18">
        <location>
            <begin position="537"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="868"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="868"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="891"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="613"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="613"/>
            <end position="617"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="617"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="653"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="654"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="654"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="654"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="765"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="765"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="933" mass="106063" checksum="B0AA16DBF8F0BAA4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZF33</accession>
    <name>A0A672ZF33_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Integrin subunit alpha 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115419414</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZF33"/>
    <dbReference type="Ensembl" id="ENSSORT00005015564.1">
        <property type="protein sequence ID" value="ENSSORP00005015103.1"/>
        <property type="gene ID" value="ENSSORG00005007573.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZF33"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000001">
        <property type="entry name" value="Integrin alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000001">
        <property type="entry name" value="Integrin subunit alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1460:FF:000001">
        <property type="entry name" value="Integrin, alpha V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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            <authorList>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="122" mass="13966" checksum="762C88B7C251B827" modified="2020-06-17" version="1">MLQRASLMTEGPRLCGNSWIFQQDNTAVHNARLTKDFFRENNITLLNHPACSPDLNPIENIWGWMAREVYKNGHQFHTVDALHEAIFTTWSNVPTSLLETLASSMPKRIFEVINKNGGATYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFW6</accession>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Neoteleostei</taxon>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <location evidence="1 8">Nucleolus</location>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:snRNA pseudouridine synthesis"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
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        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="48"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="155" mass="17138" checksum="8FD9854C9F1ED0B1" modified="2020-06-17" version="1">MTKVKKEKVSADGEEAAAAGGSERSYQDLIAYLNPIAQPLASKKLSKKLYKCVKKAAKVKNIRRGVKEVQKFINKGEKGIVVLAGDTLPIEVYCHLPVLCEDRNLPYAYIPSKMDLGTSAGTKRPTCVILIKPHEDYQEAYNECLEEVSCLPKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHQ0</accession>
    <name>A0A672ZHQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor-like protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZHQ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005017145.1">
        <property type="protein sequence ID" value="ENSSORP00005016635.1"/>
        <property type="gene ID" value="ENSSORG00005008379.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZHQ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04155">
        <property type="entry name" value="Arl3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000281">
        <property type="entry name" value="ADP-ribosylation factor-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="184" mass="20628" checksum="B2A3549591DA7AA5" modified="2020-06-17" version="1">FGQKPGLLSILRRLKHTPEQEVRLLLLGLDNAGKTTLLKQLAAEDISHITPTQGFNIKSVQSSGFKLNVWDIGGQRKIRPYWKNYFENTDVLIYVIDSSDKKRLEETSLELSELLEEEKLAAVPLLIFANKQDLTTASSASELAESLNLHTIRDRMWQVQACSALTAEGVQVLTIHSIQSAKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI74</accession>
    <name>A0A672ZI74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gramd1c</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029979738.1">
        <property type="nucleotide sequence ID" value="XM_030123878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZI74"/>
    <dbReference type="FunCoup" id="A0A672ZI74">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017343.1">
        <property type="protein sequence ID" value="ENSSORP00005016830.1"/>
        <property type="gene ID" value="ENSSORG00005008499.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411666"/>
    <dbReference type="CTD" id="54762"/>
    <dbReference type="InParanoid" id="A0A672ZI74"/>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000008">
        <property type="entry name" value="GRAM domain containing 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF1">
        <property type="entry name" value="PROTEIN ASTER-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="557"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="325"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="194"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="515"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="601"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="661" mass="75473" checksum="8A853455A951BD1F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI82</accession>
    <name>A0A672ZI82_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spi-C transcription factor (Spi-1/PU.1 related)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">spic</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZI82"/>
    <dbReference type="Ensembl" id="ENSSORT00005016705.1">
        <property type="protein sequence ID" value="ENSSORP00005016198.1"/>
        <property type="gene ID" value="ENSSORG00005008207.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZI82"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000736">
        <property type="entry name" value="transcription factor Spi-C-like isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPI-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="4">
        <location>
            <begin position="100"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="255" mass="29496" checksum="870D42566CAAFB73" modified="2020-06-17" version="1">MNFQDAIDVIQQHSNNSYYDSEYSYYETLGPPQPSLQCQISCCLVTHPSDVAAPTYDWNDTTQQSWTQVIPEVSLSHSVQTESPHFYTILPPQRKGRKKLRLYEYLHEALNDPNMGDSIQWTDSGSGTFHFISKNKEKLAECWGQRKGNRKTMTYQKMARALRNYSRTGEIIKVRRKLTYQFNPDILHRLGSAQVPMHLPCHPAQEEAHPQQPHPPEQTYCSSAAPDWHSWYGHYPLQEDYDLASSFTSHTTTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI91</accession>
    <name>A0A672ZI91_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZI91"/>
    <dbReference type="Ensembl" id="ENSSORT00005016704.1">
        <property type="protein sequence ID" value="ENSSORP00005016197.1"/>
        <property type="gene ID" value="ENSSORG00005008206.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZI91"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="52"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="419" mass="46762" checksum="7FF77A07CADABC07" modified="2020-06-17" version="1">MLDNFTTVDGADVASIIMSSKPSTCLLDPIPTKLFKQTLPLTIDTILTIINTSLVTGYVPQSFKFAVIKPLLKKPTLDPDLLVSYRPISNLPFLSKVLERVVVKQLCQHLQDNSLFEHFQSGFRAHHSTETALVKVTNDLLLAADDGLVSILVLLDLSAAFDTIDHSILLQRLEWDIGIRGAALCWFKSYLSNRYQFVNVNQQSSTYSRVSCGVPQGSVLGPILFTLYMLPLGNIIRKHGIKFHCYADDTQLYLSIKPDQTRQVDKLSSCVRDIKTWMSANYLLLNPEKTEVIIIGPKNARDSLSDQTVTLDNVSVASSTTVRNLGVLFDPDLSFKPHINQACRTAFFHLRNIAKIRNILSKNDAEKLVHAFVTSRLDHCNSLLVACPKSSLKSYQLVQNAAARLLTGTNRREHITPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJJ2</accession>
    <name>A0A672ZJJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">lars2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZJJ2"/>
    <dbReference type="Ensembl" id="ENSSORT00005017457.1">
        <property type="protein sequence ID" value="ENSSORP00005016944.1"/>
        <property type="gene ID" value="ENSSORG00005007486.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJJ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07958">
        <property type="entry name" value="Anticodon_Ia_Leu_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000003">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000011">
        <property type="entry name" value="Leucine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000100">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="71"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="15">
        <location>
            <begin position="272"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="452"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="647"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="14">
        <location>
            <begin position="737"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="911" mass="103983" checksum="1976D153450D3D04" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZML0</accession>
    <name>A0A672ZML0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">2-oxoglutarate dehydrogenase complex component E1</fullName>
            <ecNumber evidence="6">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ogdhb</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:12189"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZML0"/>
    <dbReference type="FunCoup" id="A0A672ZML0">
        <property type="interactions" value="1533"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018960.1">
        <property type="protein sequence ID" value="ENSSORP00005018416.1"/>
        <property type="gene ID" value="ENSSORG00005007830.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZML0"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000007">
        <property type="entry name" value="2-oxoglutarate dehydrogenase e1 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000001">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF7">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="23">
        <location>
            <begin position="666"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1030" mass="116586" checksum="1644219D2D96C9D2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZNB6</accession>
    <name>A0A672ZNB6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diamine acetyltransferase 1</fullName>
            <ecNumber evidence="5">2.3.1.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Polyamine N-acetyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Putrescine acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Spermidine/spermine N(1)-acetyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">sat1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an alkane-alpha,omega-diamine + acetyl-CoA = an N-acetylalkane-alpha,omega-diamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11116"/>
            <dbReference type="Rhea" id="RHEA-COMP:9766"/>
            <dbReference type="Rhea" id="RHEA-COMP:9767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:70977"/>
            <dbReference type="ChEBI" id="CHEBI:70988"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:11117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>spermidine + acetyl-CoA = N(1)-acetylspermidine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28150"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:58324"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:28151"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>spermine + acetyl-CoA = N(1)-acetylspermine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33099"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45725"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58101"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33100"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.57" evidence="5"/>
    <dbReference type="RefSeq" id="XP_030010948.1">
        <property type="nucleotide sequence ID" value="XM_030155088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZNB6"/>
    <dbReference type="FunCoup" id="A0A672ZNB6">
        <property type="interactions" value="604"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019259.1">
        <property type="protein sequence ID" value="ENSSORP00005018710.1"/>
        <property type="gene ID" value="ENSSORG00005009219.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433602"/>
    <dbReference type="InParanoid" id="A0A672ZNB6"/>
    <dbReference type="OrthoDB" id="7305308at2759"/>
    <dbReference type="UniPathway" id="UPA00188">
        <property type="reaction ID" value="UER00363"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004145">
        <property type="term" value="F:diamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019809">
        <property type="term" value="F:spermidine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009447">
        <property type="term" value="P:putrescine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032918">
        <property type="term" value="P:spermidine acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000011">
        <property type="entry name" value="Diamine acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051016">
        <property type="entry name" value="Diverse_Substrate_AcTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545:SF36">
        <property type="entry name" value="DIAMINE ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="170" mass="19777" checksum="437CDC9F3B322945" modified="2020-06-17" version="1">MASFILRKAEPKDVSDILRLIKELAKFEEMEEQVILTEKDLLEDGFGDHPFYHCLVAEVPKEQQSPEGYTVIGFAMYYFTYDPWIGKLLYLEDFYVMQDYRGLGIGSQILKVLSETAVRTRCSSMHFIVAENNTKSIEFYKRRGAADLSFEEGWRLFAIDKEDLLKMTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZPJ1</accession>
    <name>A0A672ZPJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Stress-activated protein kinase JNK</fullName>
            <ecNumber evidence="5">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulates transcriptional activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZPJ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005019632.1">
        <property type="protein sequence ID" value="ENSSORP00005019076.1"/>
        <property type="gene ID" value="ENSSORG00005009394.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZPJ1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000508">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008351">
        <property type="entry name" value="MAPK_JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01772">
        <property type="entry name" value="JNKMAPKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="166"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="224" mass="25138" checksum="23E1C4AB21035ACC" modified="2020-06-17" version="1">MGSAIYHDIPFHNTVSILKSTVWIFVGIYSNADMAWVHFCFSLLCIFYFFLFHTLLFNNVGSFVGIELKSCSDVTLLRHFSCFCFRTTASQARDLLSKMLIIDPAKRISVDEALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHSIDEWKELIYKEVMNFEERTKNGVVKGQPSPSGAAVNSSESLPPSSSINDISSMSTDQTLASDTDSSLETSAGPLGCCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVL5</accession>
    <name>A0A672ZVL5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ERCC4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZVL5"/>
    <dbReference type="Ensembl" id="ENSSORT00005021656.1">
        <property type="protein sequence ID" value="ENSSORP00005021029.1"/>
        <property type="gene ID" value="ENSSORG00005010264.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZVL5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="6">
        <location>
            <begin position="322"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="226"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="507" mass="56103" checksum="8E35F16D7EB140EC" modified="2020-06-17" version="1">MNRKRVTALDTSNETEDNAVSDDDFQNESVLIRRPITAEKHRVQQANAKRSAIRRRHQFLDEEAELLDDDDAADVSSDEEDGEDQNQSLEGFVVDNSHFSQGLNDSEMQGIYLKSVRSPAVRGKFKMSHRNHHNMDIFSQVPEVDETYAEDSFVVGSDEEELENSEEDDNEEEEGVELLPEESYVDGRRQYATRRRVFLHKARAGAETKAERPLVHRGGVKAKRARVIRVEDSSDEETEQVSEDASGTAGGRGVANLSSKSAEQEQKSTSFTSSTSTIASKVSLLSKAQRGATVPGSSSSSSLVPQPQMSTSRSPAPPGPVYILVDSRCLSGGVELMTSLRQRHSASVHICSLDGGYFIVSNRMAVERHSQSDLASMQNRKRLVDRVGGLQGLFERVCLIIEKDRTKPGEASRPFQRTRYYDSTVAALVRSGVRLLWSGGAEESAGLLAELAKLEQRKAQGITVPLEVKGQHRQQALQLYLSLPSVNYVHALNMSHNFGSVAQLINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZL7</accession>
    <name>A0A672ZZL7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exocyst complex component 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Exocyst complex component Sec6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">exoc3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with EXOC3L1. Interacts with BIRC6/bruce. Interacts with MYRIP. Interacts with SLC6A9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030015396.1">
        <property type="nucleotide sequence ID" value="XM_030159536.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030015397.1">
        <property type="nucleotide sequence ID" value="XM_030159537.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030015398.1">
        <property type="nucleotide sequence ID" value="XM_030159538.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030015399.1">
        <property type="nucleotide sequence ID" value="XM_030159539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZZL7"/>
    <dbReference type="Ensembl" id="ENSSORT00005023330.1">
        <property type="protein sequence ID" value="ENSSORP00005022665.1"/>
        <property type="gene ID" value="ENSSORG00005010998.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436638"/>
    <dbReference type="CTD" id="11336"/>
    <dbReference type="InParanoid" id="A0A672ZZL7"/>
    <dbReference type="OrthoDB" id="10047020at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000004">
        <property type="entry name" value="Exocyst complex component 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.70:FF:000001">
        <property type="entry name" value="Exocyst complex component 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF13">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="751" mass="86773" checksum="FF0F7FEB5B4D649B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A121</accession>
    <name>A0A673A121_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coagulation factor IX</fullName>
            <ecNumber evidence="4">3.4.21.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Christmas factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">f9b</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.</text>
            <dbReference type="EC" id="3.4.21.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.22" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673A121"/>
    <dbReference type="FunCoup" id="A0A673A121">
        <property type="interactions" value="793"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023662.1">
        <property type="protein sequence ID" value="ENSSORP00005022991.1"/>
        <property type="gene ID" value="ENSSORG00005011137.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A121"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:proteolysis"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Tryp_SPc"/>
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        <property type="entry name" value="Coagulation factor X (Predicted)"/>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
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        <property type="entry name" value="GLA-like_dom_SF"/>
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        <property type="entry name" value="GLA_domain"/>
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    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
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        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
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        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF31">
        <property type="entry name" value="COAGULATION FACTOR IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Trypsin"/>
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        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
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    </dbReference>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GLA_2"/>
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        <property type="entry name" value="TRYPSIN_DOM"/>
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        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="20 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 25" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="14 26" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="22" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 26" id="KW-0645">Protease</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="15 26" id="KW-0720">Serine protease</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Gla" evidence="29">
        <location>
            <begin position="22"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="27">
        <location>
            <begin position="68"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="28">
        <location>
            <begin position="264"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
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            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00031357"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="506" mass="57051" checksum="101E0FD2DEDD7157" modified="2020-06-17" version="1">SGSVFLSQQSAHTVLRRQRRYNSGKLEEVLYKANLERECREEVCNMEEAREWFENDEKTMEFWAGYIDGNQCDPPPCQNGGVCTDGVNSYVCWCKQAYSGKNCEIEVAKQCSFNNGGCSHFCVMRGDQAMCQCAYGYRLLPDRKSCEPTGLKNITNNLTTAALTQPTNASERIHNSSDALQDEIYDSNATYLYDYYELARNDSDPYSDFDVPAVDVRSAKSDPITYDGAAVSVTDIPTEREEIPAWAFPTLPTIVATGNVDQKIVGGTEAIRGEFPWQVALMSRSHPQHRALPFCGGSLLTEVWVITAAHCLTELNNAKKGEHDVLRDEGPERDYMVEEQHVHPLYNHEKSLYNHDIALLKLASPVELSRERRPICLGPKDFTENLLRDASTSMVSGWGRLKFQGPEATKLQKLAVPYVDRTTCKQSSREQVTRYMFCAGYRTQQMDSCQGDSGGPHATEYKGTWFLTGIVSWGEECAMDGKYGIYTRVSRYYPWISHKTGIRVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6C0</accession>
    <name>A0A673A6C0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="12">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">shmt2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 12">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A673A6C0"/>
    <dbReference type="FunCoup" id="A0A673A6C0">
        <property type="interactions" value="1006"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024918.1">
        <property type="protein sequence ID" value="ENSSORP00005024207.1"/>
        <property type="gene ID" value="ENSSORG00005009389.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A6C0"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000048">
        <property type="entry name" value="Serine hydroxymethyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF28">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="13">
        <location>
            <begin position="49"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="503" mass="55720" checksum="09A2758F4986F88F" modified="2020-06-17" version="1">LVKDRYVKICLSTPLCQRAPVRLCVRGQQSHAATRVVDEERPWTGQESLAQDDPEMWDLLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEMVDQIELLCQKRALEAFDLDPAQWGVNVQPYSGSPANFAAYTAILNPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIYFESMPYKLNPATGLIDYDQMEMTARLFRPKLIIAGTSAYARLIDYARIKKLCTEVKAYLLADMAHISGLVAAQAIPSPFDYADLVTSTTHKSLRGARAGLIFYRKGVRSVDKKGKEILYDLEDRVNFSVFPSLQGGPHNHAIAGVAVALKQAQSPMFKEYIAQVIKNSKAMAVALLSKGYTLVSGGTDNHLVLVDLRPKGIDGARAERVLELVSITANKNTCPGDKSALTPGGLRLGAPALTSRQFKEADFVQVVEFMDEGFKIALDVKKKTGKLQDFKNFLLQDPETVARIAELRQRVEAFARPFPMPGFHDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AB85</accession>
    <name>A0A673AB85_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Potassium voltage-gated channel, subfamily H (eag-related), member 5b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AB85"/>
    <dbReference type="Ensembl" id="ENSSORT00005027220.1">
        <property type="protein sequence ID" value="ENSSORP00005026444.1"/>
        <property type="gene ID" value="ENSSORG00005012668.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AB85"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000035">
        <property type="entry name" value="Potassium voltage-gated channel, subfamily H (Eag-related), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003949">
        <property type="entry name" value="K_chnl_volt-dep_EAG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF533">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY H MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01464">
        <property type="entry name" value="EAGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="406" mass="46765" checksum="81B78A1D22811EB6" modified="2020-06-17" version="1">YQEMSFFMYGELTDKKTIDKVRQTFDNYESNCFEILLYRKNRSPVWFYMQVAPIRNENDKVVLFLCTFKDITLFKQPIEDETTKGWTKFARLTRALTNNRNTLQQLAPISKHEVSHKQSRLAEVLQLNSDILPQYKQEAPKTPPHIILHYCTFKTTWDWVILILTFYTAIMVPYNVSFKTKQNNIVWLVLDSVVDVIFLVDIVLNFHTTFVGPGGEVISDPKLIRMNYLKTWFVIDLLSCLPYDIINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYLEYGAAVLVLLVCVFGLVAHWLACIWYSIGDYEVIDEATNTIKKDSWLYQLAISIGSPYRYNASGSGQWEGGPGKDSLYITSLYFTMTSLTTIGFGNIAPTTDGEKIFSVAMMMVGCESQFWCIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AC47</accession>
    <name>A0A673AC47_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Twinkle mtDNA helicase</fullName>
            <ecNumber evidence="15">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Twinkle protein, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">twnk</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Mitochondrial helicase involved in mtDNA replication and repair. Might have a role in mtDNA repair. Has DNA strand separation activity needed to form a processive replication fork for leading strand synthesis which is catalyzed by the formation of a replisome complex with POLG and mtSDB. Preferentially unwinds DNA substrates with pre-existing 5'-and 3'- single-stranded tails but is also active on a 5'- flap substrate. Can dissociate the invading strand of immobile or mobile D-loop DNA structures irrespective of the single strand polarity of the third strand. In addition to its DNA strand separation activity, also has DNA strand annealing, DNA strand-exchange and DNA branch migration activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="15"/>
    <dbReference type="RefSeq" id="XP_029977548.1">
        <property type="nucleotide sequence ID" value="XM_030121688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AC47"/>
    <dbReference type="FunCoup" id="A0A673AC47">
        <property type="interactions" value="1033"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027557.1">
        <property type="protein sequence ID" value="ENSSORP00005026774.1"/>
        <property type="gene ID" value="ENSSORG00005012830.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410192"/>
    <dbReference type="CTD" id="56652"/>
    <dbReference type="InParanoid" id="A0A673AC47"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01122">
        <property type="entry name" value="Twinkle_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000008">
        <property type="entry name" value="Mitochondrial helicase twinkle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000845">
        <property type="entry name" value="Mitochondrial helicase twinkle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007694">
        <property type="entry name" value="DNA_helicase_DnaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027032">
        <property type="entry name" value="Twinkle-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873">
        <property type="entry name" value="T7-LIKE MITOCHONDRIAL DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12873:SF0">
        <property type="entry name" value="TWINKLE MTDNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13481">
        <property type="entry name" value="AAA_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51199">
        <property type="entry name" value="SF4_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="SF4 helicase" evidence="21">
        <location>
            <begin position="414"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="670"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="680"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="703"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="718" mass="81505" checksum="00ADC05211EC1657" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACI0</accession>
    <name>A0A673ACI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115436797</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Choline transporter.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Choline/H+ antiporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>choline(out) + n H(+)(in) = choline(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75463"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ACI0"/>
    <dbReference type="FunCoup" id="A0A673ACI0">
        <property type="interactions" value="810"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027740.1">
        <property type="protein sequence ID" value="ENSSORP00005026956.1"/>
        <property type="gene ID" value="ENSSORG00005012272.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ACI0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF42">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="443"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="489"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="525"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="694" mass="78770" checksum="E98AE74795E7B002" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADF3</accession>
    <name>A0A673ADF3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Redox-regulatory protein FAM213A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC115410615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ADF3"/>
    <dbReference type="Ensembl" id="ENSSORT00005027571.1">
        <property type="protein sequence ID" value="ENSSORP00005026788.1"/>
        <property type="gene ID" value="ENSSORG00005012836.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ADF3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8008" checksum="90C6364BA6C77C6E" modified="2020-06-17" version="1">IRLWLTTASAIGGFITGVLNSVTDRFLTPPLKATLKHLEETELKTLSGEKKAFKAKLLWEKSGAVIMAVRRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AE83</accession>
    <name>A0A673AE83_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Neoverrucotoxin subunit beta-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115415570</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029985055.1">
        <property type="nucleotide sequence ID" value="XM_030129195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AE83"/>
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        <property type="protein sequence ID" value="ENSSORP00005027569.1"/>
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            <dbReference type="PROSITE" id="PS50824"/>
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            <dbReference type="Ensembl" id="ENSSORP00005027569.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="304" mass="34480" checksum="A9D51AF97294A43B" modified="2020-06-17" version="1">MEKQVLFGILNELSNEEFETFKWFLKDRSFLKNFPAIPNTKLTETKLPIHLVDVMVQTYVKNAIEVTRMTLKVINRQDLLEQLPESMPEPAGASRLNPAVAECGPHRMDTGRVDRSGSQMSKPGLKKYFYEMILDINSAHRFLKLSHNNRKAECASEVIQPYPDHPDRFDIWCQVLCSTALTSRCYWEVEWSGEVQIAVTYRGINRKGGSHDCVVGENPQSWSLWCSADGHSVRHNKIKSILPHSSLSSGRIAVDLDYSAGSLSFYKVSSGDLIHLHTYNTTFTEPLYAGFWFGPRSSVSLCDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AF11</accession>
    <name>A0A673AF11_9TELE</name>
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        <submittedName>
            <fullName evidence="12">Mannose receptor, C type 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115437379</name>
    </gene>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Single-pass membrane protein</topology>
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        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
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        <property type="entry name" value="C-type lectin-like"/>
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        <location>
            <begin position="1323"/>
            <end position="1352"/>
        </location>
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            <end position="200"/>
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            <end position="316"/>
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        </location>
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        <source>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1404" mass="158427" checksum="8C5286AB4D8C7EEA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGH8</accession>
    <name>A0A673AGH8_9TELE</name>
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        <submittedName>
            <fullName evidence="13">Homeobox and leucine zipper protein Homez-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">homeza</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AGH8"/>
    <dbReference type="Ensembl" id="ENSSORT00005028615.1">
        <property type="protein sequence ID" value="ENSSORP00005027818.1"/>
        <property type="gene ID" value="ENSSORG00005013310.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGH8"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000172">
        <property type="entry name" value="Homeobox and leucine zipper protein Homez"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024578">
        <property type="entry name" value="Homez_homeobox_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15467:SF10">
        <property type="entry name" value="HOMEOBOX AND LEUCINE ZIPPER ENCODING B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15467">
        <property type="entry name" value="ZINC-FINGERS AND HOMEOBOXES RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11569">
        <property type="entry name" value="Homez"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="93"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="256"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="486"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="95"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="258"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="488"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="156"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="390"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="559"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="450"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="641" mass="71488" checksum="7D0CCD453B57C08B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AH17</accession>
    <name>A0A673AH17_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ethanolamine kinase</fullName>
            <ecNumber evidence="5">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115420984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673AH17"/>
    <dbReference type="Ensembl" id="ENSSORT00005028436.1">
        <property type="protein sequence ID" value="ENSSORP00005027642.1"/>
        <property type="gene ID" value="ENSSORG00005013187.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AH17"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF68">
        <property type="entry name" value="ETHANOLAMINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="340" mass="38738" checksum="F97CE8B0F9501E36" modified="2020-06-17" version="1">MSGGTLASDNVLLSHSGHSGHLLHVEIHIDEHEPRRGILELLRRLRPQWNSGEIQMKTFTDGITNQLIGCFVGSLQEPGCVLVRLYGRKTELFVNRDREVEMFQVFHAHGCGPQIYCSFQNGICYEFVRGTVLDDELLRQPSIYRLIAAEMGRVHAIRPKSGPPVKPFLWTKMACFLKLLQSSNITLPSLETLRMEMESLKTHLSQIESPTVLCHNDLLTKNIIYNQEEGTVKFIDYEYADYNYQAFDIGNHFNEFAGVSVVDYSLYPSLDLQRDWLKAYLQSYKLSGGQEDTVTEEEVTQLYVHVCKFSLVMLPKLNTICGIVQLRGTDGANVSEIDWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHW6</accession>
    <name>A0A673AHW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AHW6"/>
    <dbReference type="Ensembl" id="ENSSORT00005028737.1">
        <property type="protein sequence ID" value="ENSSORP00005027937.1"/>
        <property type="gene ID" value="ENSSORG00005013376.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AHW6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF28">
        <property type="entry name" value="ZINC FINGER PROTEIN 37-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="226" mass="26591" checksum="D1929E8C1BC415F8" modified="2020-06-17" version="1">HVIVKEEFLIEEDEEDLQFLRLHQRRREEPDRQDGRSEPERYIGMRTHGKAESSDTEDICGMRFAQKSILLGHVRVHTGEKPFSCSICKKSFKNKYYIRTHMLTHTGEKPFSCTICHERFSQKQNLLNHVMRHKGEKPFSCPVCKRSFTQKVHADTHMLTHTGEKPFSCPICRRPFAQKAHVRSHMRIHTKPRRPSTQRHSFTLVGGLKYLMNSEVRHCFLNVSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKF7</accession>
    <name>A0A673AKF7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibroblast growth factor</fullName>
            <shortName evidence="3">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115426585</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000547.1">
        <property type="nucleotide sequence ID" value="XM_030144687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AKF7"/>
    <dbReference type="Ensembl" id="ENSSORT00005029973.1">
        <property type="protein sequence ID" value="ENSSORP00005029143.1"/>
        <property type="gene ID" value="ENSSORG00005013911.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426585"/>
    <dbReference type="InParanoid" id="A0A673AKF7"/>
    <dbReference type="OrthoDB" id="6158176at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000004">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="229" mass="25371" checksum="50CC0D2399D26D58" modified="2020-06-17" version="1">MGAAAEVGSFANVFLDSFGPAGAASAPVGSHFVLTPAGAVDFNSVSGLMMGVQGQQQQQEHLVSAERLSRSLADLSHLKGILRRRQLYCRTGFHLEIHPDGTVQGTRKDHSRFGILEFISLAVGLVSIRGVDSGLYLAMNSKGELYGSEKLSAECVFREQFEENWYNTYSSNIYRHGEKGAFYFVALNKDGTSRDGTRSRRHQRFTHFLPRPVDPDRVPELYRDILGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASY1</accession>
    <name>A0A673ASY1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Immunoglobulin-like domain-containing receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115416393</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. LISCH7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029986015.1">
        <property type="nucleotide sequence ID" value="XM_030130155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ASY1"/>
    <dbReference type="Ensembl" id="ENSSORT00005032294.1">
        <property type="protein sequence ID" value="ENSSORP00005031417.1"/>
        <property type="gene ID" value="ENSSORG00005014941.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416393"/>
    <dbReference type="InParanoid" id="A0A673ASY1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051874">
        <property type="entry name" value="Ig-like_domain-LISCH7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008664">
        <property type="entry name" value="LISCH7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15923:SF7">
        <property type="entry name" value="IMMUNOGLOBULIN-LIKE DOMAIN-CONTAINING RECEPTOR 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15923">
        <property type="entry name" value="TRANSMEMBRANE AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05624">
        <property type="entry name" value="LSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="61"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="404"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="552"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="440"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="483"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="563"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="691"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="702"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="719"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="744" mass="83472" checksum="4F89934FCED4400C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATC5</accession>
    <name>A0A673ATC5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Caveolin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cav3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <location evidence="10">Caveola</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the caveolin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ATC5"/>
    <dbReference type="FunCoup" id="A0A673ATC5">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032447.1">
        <property type="protein sequence ID" value="ENSSORP00005031567.1"/>
        <property type="gene ID" value="ENSSORG00005015021.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATC5"/>
    <dbReference type="OrthoDB" id="5917823at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070836">
        <property type="term" value="P:caveola assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001612">
        <property type="entry name" value="Caveolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018361">
        <property type="entry name" value="Caveolin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844:SF16">
        <property type="entry name" value="CAVEOLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01146">
        <property type="entry name" value="Caveolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01210">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="152" mass="17489" checksum="379C920E27AC1E95" modified="2020-06-17" version="1">MADQYQYNTNEEKIVKDSHTKEIDLINRDPKQINEDVVKVEFEDVIAEPDGTHSLDGVWKLSYTTFTVSKYWCYRILSAVFGIPVALLWGFLFACISFCHIWAVVPCIKSCLIESQCVSRIYSLCIQTFCDPFFEALSKICSNVRVALRKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATV3</accession>
    <name>A0A673ATV3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Olfactory receptor 52E8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115412556</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029980986.1">
        <property type="nucleotide sequence ID" value="XM_030125126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ATV3"/>
    <dbReference type="Ensembl" id="ENSSORT00005033925.1">
        <property type="protein sequence ID" value="ENSSORP00005033021.1"/>
        <property type="gene ID" value="ENSSORG00005015658.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412556"/>
    <dbReference type="InParanoid" id="A0A673ATV3"/>
    <dbReference type="OrthoDB" id="6151005at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF871">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="40"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="35253" checksum="5CF4871B6BB9122D" modified="2020-06-17" version="1">MMDNFSVITVFTLSGLSGIEKYKISLFFVTLLFYCVIWLVNVAVIVTIVVDKSLHEPMYIFLCNLCINGLYGTAGFYPKFLMDLLSTTHVISYAGCLLQGFVIHSSVSADFSLLVLMAFDRYVAICRPLVYHSMMTKPRVCVFLFFAWFIPIYLVFVGSMTTLTLRLCGSHIPKIYCINYLVGQLACSFSVAHVIVPAFNYSFFSCHFIFVIWSYIYLVKTCRTSRENRSKFIQTCLPHLFCLVTFGVSLLFDLLYIRFGSKSSSQSAQNFMAIEFLIIPPILNPLIYGFKMTQIRKRIIQFLIGKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1D2</accession>
    <name>A0A673B1D2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyadenylate-binding protein-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">paip2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a repressor in the regulation of translation initiation of poly(A)-containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. Displaces the interaction of PABPC1 with poly(A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly(A) RNP structure organization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAIP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002095.1">
        <property type="nucleotide sequence ID" value="XM_030146235.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030002096.1">
        <property type="nucleotide sequence ID" value="XM_030146236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B1D2"/>
    <dbReference type="Ensembl" id="ENSSORT00005035257.1">
        <property type="protein sequence ID" value="ENSSORP00005034338.1"/>
        <property type="gene ID" value="ENSSORG00005016236.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427622"/>
    <dbReference type="InParanoid" id="A0A673B1D2"/>
    <dbReference type="OrthoDB" id="5985142at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040396">
        <property type="entry name" value="PAIP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13154">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13154:SF2">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="133" mass="15449" checksum="3B7A42CD4EBC833D" modified="2020-06-17" version="1">MKDPSLSNSSPNMTISNEVILSNQLISDDNPFAEYMWMENEEEFNRQVEEELWEEEFIECCFQEMLEEEEQWEWFIPSRDLPSAQTVSQLQDQIGLLVLDADIHADADIDVVVHSSLNPNAKEFTPGIQKHVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1V1</accession>
    <name>A0A673B1V1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tripartite motif-containing protein 54</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115437208</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May bind and stabilize microtubules during myotubes formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_030016276.1">
        <property type="nucleotide sequence ID" value="XM_030160416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B1V1"/>
    <dbReference type="FunCoup" id="A0A673B1V1">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035413.1">
        <property type="protein sequence ID" value="ENSSORP00005034493.1"/>
        <property type="gene ID" value="ENSSORG00005016279.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437208"/>
    <dbReference type="InParanoid" id="A0A673B1V1"/>
    <dbReference type="OrthoDB" id="5351233at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19833">
        <property type="entry name" value="Bbox2_MuRF3_C-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16760">
        <property type="entry name" value="RING-HC_MuRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000014">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase MID2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033492">
        <property type="entry name" value="Trim54_Bbox2_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF17">
        <property type="entry name" value="TRIPARTITE MOTIF CONTAINING 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="15" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="10"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="20">
        <location>
            <begin position="107"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="COS" evidence="21">
        <location>
            <begin position="257"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="317"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="452" mass="49925" checksum="169E92DD7248F52B" modified="2020-06-17" version="1">MDSLEKQLICPICLELFSKPVVILPCQHNLCRKCANDVFQASNPYLSTRSGSTVTSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDMYKQGSASSKPGPEPKVDQPMCDHHEDEKINIYCVSHSVPTCSLCKVFGVHKDCEVAPLDNVFQKQKTELTDCVSMLVGNNDRIQAIISQLDETCRAVDENGRRQKSKVCEAFDHLFALLEERKGDMTLRINAEQEEKLHYIGSLRRKYSEHLENGAKLLETAIQTMDQSEMALFLQTAKPLLQKIVEGTNTSHLDKIQPGYENMDHFSTNFNPQRRALNTVDFVKLDEDDDDEDEVEVRGMAAGSRSEAPPPLGTALAPPPPLLPMGAQLVAPPSSSQKPLEAPPTPTKSPTPTAAPPLLPQRLSELLPTPTKSPTPTAAPPPLPVPAAGPTPTVQSEQQSESDNRDGPKHVFSFSWLNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4R6</accession>
    <name>A0A673B4R6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="2">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">cars1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:17774"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="2"/>
    <dbReference type="RefSeq" id="XP_030017543.1">
        <property type="nucleotide sequence ID" value="XM_030161683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B4R6"/>
    <dbReference type="FunCoup" id="A0A673B4R6">
        <property type="interactions" value="1615"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036438.1">
        <property type="protein sequence ID" value="ENSSORP00005035488.1"/>
        <property type="gene ID" value="ENSSORG00005011454.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438237"/>
    <dbReference type="CTD" id="833"/>
    <dbReference type="InParanoid" id="A0A673B4R6"/>
    <dbReference type="OrthoDB" id="438179at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10310">
        <property type="entry name" value="GST_C_CysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="15">
        <location>
            <begin position="117"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="728"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="776"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="728"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="821" mass="93882" checksum="65462656ACE00376" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5Y2</accession>
    <name>A0A673B5Y2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">72 kDa type IV collagenase</fullName>
            <ecNumber evidence="4">3.4.24.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">72 kDa gelatinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Matrix metalloproteinase-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">mmp2</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.</text>
            <dbReference type="EC" id="3.4.24.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="23">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.24" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673B5Y2"/>
    <dbReference type="Ensembl" id="ENSSORT00005037015.1">
        <property type="protein sequence ID" value="ENSSORP00005036057.1"/>
        <property type="gene ID" value="ENSSORG00005017012.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5Y2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048771">
        <property type="term" value="P:tissue remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000002">
        <property type="entry name" value="72 kDa type IV collagenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000010">
        <property type="entry name" value="72 kDa type IV collagenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000002">
        <property type="entry name" value="Matrix metallopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF29">
        <property type="entry name" value="72 KDA TYPE IV COLLAGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0177">Collagen degradation</keyword>
    <keyword evidence="18 24 27" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 29" id="KW-0732">Signal</keyword>
    <keyword evidence="13 23" id="KW-0862">Zinc</keyword>
    <keyword evidence="17" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="29">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025328056" description="72 kDa type IV collagenase" evidence="29">
        <location>
            <begin position="30"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="30">
        <location>
            <begin position="234"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="30">
        <location>
            <begin position="292"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="30">
        <location>
            <begin position="350"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="471"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="516"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="564"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="613"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="26">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="active site" evidence="22">
        <location>
            <position position="237"/>
        </location>
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    <sequence length="659" mass="74671" checksum="9653FD63B6FAD7E6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BA08</accession>
    <name>A0A673BA08_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">DNA excision repair protein ERCC-1</fullName>
        </recommendedName>
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        <name evidence="11" type="primary">ercc1</name>
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    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="10">
        <location>
            <begin position="155"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="111"/>
            <end position="141"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071993"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="356" mass="39319" checksum="7F852A1CB27DDA89" modified="2020-06-17" version="1">AMKKRFNINLNDSVFTKERTPPKPQFPSCKGGQHITAPTTSSSNPAPVPMDQPLSYAEYNIQSKTNVAAPVHRDCSSNQPRTEADGVSHVKHSDRHSESSRTGGGDPAENSAKRTESGCVELKNETAGPDKSEEESQRSDLGHGMGPKPVGSGSSIIVSPRQRGNPILKFVRSVPWEFGEVVPDYVLGQTTCALFLSLRYHNLNPNYIHDRLKQLGQTFTLRVLLVQVDVKDPHHALKELARICIMADCTLILAWSPEEAGRYLETYKSYEKKPADLLKEQVEKDYLSKVTDCMTTVKSINKTDAITLLSAFSSIEGIIEASKEDLALCPGLGPQKARRLHDVLHKPFLKSKTKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BA44</accession>
    <name>A0A673BA44_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BA44"/>
    <dbReference type="Ensembl" id="ENSSORT00005039160.1">
        <property type="protein sequence ID" value="ENSSORP00005038169.1"/>
        <property type="gene ID" value="ENSSORG00005017867.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BA44"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007951">
        <property type="entry name" value="KRTAP_PMG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05287">
        <property type="entry name" value="PMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="115" mass="12721" checksum="B0F35CDC676C40FF" modified="2025-10-08" version="2">MCQDSRSCQSKSCQSKSCQSRSCQSRSCQSRSCQSRSCQRRSCQSRSCQSGSCQSGSFQSKSCQSSKSCQSRSCQSRSCQSRSFQSRSCQSRSCQTEVLSGSTFRPVETPVEPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BE66</accession>
    <name>A0A673BE66_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Galactose-specific lectin nattectin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115438516</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BE66"/>
    <dbReference type="FunCoup" id="A0A673BE66">
        <property type="interactions" value="788"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040574.1">
        <property type="protein sequence ID" value="ENSSORP00005039564.1"/>
        <property type="gene ID" value="ENSSORG00005018446.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BE66"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025418325" evidence="3">
        <location>
            <begin position="20"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="83"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="201" mass="22391" checksum="B3762C7589FC1708" modified="2020-06-17" version="1">MKLLVVFLLVGTMMVLSEGFPEPEPGPEAESSEESEPETESPDDESPDNESPDDGSPDEESPDEDKNSVVKRAVGCPFGWTQFGRRCLIVNAPMKWTDAQSRCLSMKGNLLSIHSVHQNNWAVSLMGGRRAWIAGSDAQEEGQWFNVDGTRFNYANWCHGEPNNHGGQEHCAEINYSDQRCWNDAPCLMHLHSICARKVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BGF5</accession>
    <name>A0A673BGF5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tumor necrosis factor ligand superfamily member 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tnfsf12</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BGF5"/>
    <dbReference type="FunCoup" id="A0A673BGF5">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040417.1">
        <property type="protein sequence ID" value="ENSSORP00005039408.1"/>
        <property type="gene ID" value="ENSSORG00005018392.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BGF5"/>
    <dbReference type="OrthoDB" id="6159739at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051748">
        <property type="entry name" value="TNF_Ligand_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15151">
        <property type="entry name" value="PROTEIN EIGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15151:SF20">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="THD" evidence="7">
        <location>
            <begin position="105"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="247" mass="27772" checksum="1A0AE897E2BE5E75" modified="2020-06-17" version="1">MQRILQRRRVRRLRVLWASLALLALALAACSALFTAWTWRQTRDLSQSFQILQDRLEQVNTQRKAIVQLILEKRELLVGQRVKRDGGTLRGGRSGNGKKAASHFEITKVSSQQVGDGGVIKGWEERTLNMSKAVRYNKEQGTFTVEKAGVYFLFCQVLFNEEKSQYVKLDVKTIGQKPQKLQCMEGYGTTPSAGPHPFHFLKPCQVSGLLRLDKGTELQAITGASFRLHPVGNTSASPHVFSIFKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIZ2</accession>
    <name>A0A673BIZ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Troponin T type 2a (cardiac)</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BIZ2"/>
    <dbReference type="Ensembl" id="ENSSORT00005041347.1">
        <property type="protein sequence ID" value="ENSSORP00005040308.1"/>
        <property type="gene ID" value="ENSSORG00005018806.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BIZ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:troponin C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031013">
        <property type="term" value="F:troponin I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF5">
        <property type="entry name" value="TROPONIN T, CARDIAC MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="178" mass="21908" checksum="D54B755653BCB082" modified="2020-06-17" version="1">MEKDLQELQTLIEVHFESRKKEEDELIQLKDRIEKRRSERAEQQRIRSEREKERQKRLEEERTRKEEEEAKKRAEDDAKKKKTLTSLHFGGYMQKLVKKRSGKRQTEREKKKKILSERRKPLDIDNLSQDRLKDKAKELWDWMHELEAEKFELQYQFTRQKYEVCVILDMISNTSEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKF9</accession>
    <name>A0A673BKF9_9TELE</name>
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        <submittedName>
            <fullName evidence="5">Thioredoxin domain containing 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">txndc16</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BKF9"/>
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        <property type="interactions" value="737"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042841.1">
        <property type="protein sequence ID" value="ENSSORP00005041769.1"/>
        <property type="gene ID" value="ENSSORG00005019400.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BKF9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040090">
        <property type="entry name" value="TXNDC16"/>
    </dbReference>
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        <property type="entry name" value="TXNDC16_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057645">
        <property type="entry name" value="TXNDC16_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057639">
        <property type="entry name" value="TXNDC16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22699">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22699:SF1">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24509">
        <property type="entry name" value="TXNDC16_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TXNDC16_N"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025661813" evidence="1">
        <location>
            <begin position="27"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="TXNDC16 N-terminal" evidence="2">
        <location>
            <begin position="9"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="TXNDC16 second thioredoxin-like" evidence="3">
        <location>
            <begin position="85"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="TXNDC16 third thioredoxin-like" evidence="4">
        <location>
            <begin position="211"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="657" mass="73447" checksum="13F65AF03DCD199A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKM7</accession>
    <name>A0A673BKM7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ubiquitin-conjugating enzyme E2 J1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">E2 ubiquitin-conjugating enzyme J1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Non-canonical ubiquitin-conjugating enzyme 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ube2j1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) and is essential for cells to recover from ER stress. Plays a role in MAPKAPK2-dependent translational control of TNF-alpha synthesis. Also acts as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the E3 ubiquitin-protein ligase RNF26. Plays a role in male fecundity through the interaction with the E3 ubiquitin-protein ligase RNF133.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the HRD1 complex, which comprises at least SYNV1/HRD1, DERL1/2, FAM8A1, HERPUD1/HERP, OS9, SEL1L and UBE2J1. Interacts with E3 ligase RNF26. Interacts with E3 ligase RNF133.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029983672.1">
        <property type="nucleotide sequence ID" value="XM_030127812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BKM7"/>
    <dbReference type="FunCoup" id="A0A673BKM7">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043403.1">
        <property type="protein sequence ID" value="ENSSORP00005042325.1"/>
        <property type="gene ID" value="ENSSORG00005019618.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414593"/>
    <dbReference type="CTD" id="51465"/>
    <dbReference type="InParanoid" id="A0A673BKM7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000043">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="19">
        <location>
            <begin position="14"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="191"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="331" mass="36449" checksum="DD8CFEBB1D23C9AA" modified="2020-06-17" version="1">MSGNMENKYNLKSPAVKRLMKEAVELREPTEHYHAQPLEDNLFEWHFSVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTPNGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERKALAKKSVDFCCDICGCSMSSALLPLSANSRCSTDDHKAKELAQQINFKAESSLSRQASECRGSGNEPPTPPQGDTTRSAGPEHADRSALDEQAEASPASRVEGPPTPPVPTTSRPLSPRQRRTQHHSQQGQRDSREAPNRPAFAAAAHQPQEESHAGSAVLIVVLTLALAALIFRRIYLAHEYKFDYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BN67</accession>
    <name>A0A673BN67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BN67"/>
    <dbReference type="Ensembl" id="ENSSORT00005043129.1">
        <property type="protein sequence ID" value="ENSSORP00005042057.1"/>
        <property type="gene ID" value="ENSSORG00005019503.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BN67"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF125">
        <property type="entry name" value="SODIUM-DEPENDENT NEUTRAL AMINO ACID TRANSPORTER B(0)AT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="269" mass="30550" checksum="8CB6325111D4AC68" modified="2020-06-17" version="1">MKLKIRLPNPGLDDRIPSHKDLEDMEKQEAADRPQWDNKAQYLLTCVGFCVGLGNVWRFPYLCQSHGGGAFMIPFLFLLIFEGIPLLYLEFAIGQRLRKGSVAVWTAINPYLAGVGIASMCVSFLVGMYYNTIIAWVMWYFFHSFQSPLPWSQCPLNDNRTGLVSECKRSSSIDYFWYRETLNTSETIDVSGGLQWWMVLCLFCAWAVLYICCIRGIETTGKAVYITSTLPYVVLTIFLIRGLTLKGSVDGIKFLFTPRVSILINIDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BP36</accession>
    <name>A0A673BP36_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BP36"/>
    <dbReference type="Ensembl" id="ENSSORT00005044623.1">
        <property type="protein sequence ID" value="ENSSORP00005043525.1"/>
        <property type="gene ID" value="ENSSORG00005020110.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BP36"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002274">
        <property type="entry name" value="Si:dkeyp-69e1.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF148">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0290503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="55"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="309" mass="34639" checksum="A4029A43E6081FC7" modified="2020-06-17" version="1">MEAADKNCTLDSSTDGGFAVRCTEEVLLPVFTDESLQEAMEFQPPLLSNTIPPVLPEIRLVLLGRKGAGKSAAGNTILGNVGGFESGKPTEECVKRRADVDGRKVTVVDTPGWEWYYPLNSTPTWVKRETLRSVSLCPPGPHAVLLVVRSCASVTKDYIREIEEHLEPLGKGVWDHIMVLFTRGDELGLGSMEQRIQTTGPGLQTLLQKCGYRYHVVDNRSRGDGTQVKELIRKLEVMVEGKEERSKHLEMDSMVLLGLEADAKRRARERRKKQRQMEAQTQRVIIKASVMSKFFRHVESFQTHAHSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRD5</accession>
    <name>A0A673BRD5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein-glutamine gamma-glutamyltransferase</fullName>
            <ecNumber evidence="6">2.3.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">f13a1b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N(6)-5-L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:54816"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:14005"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:138370"/>
            <dbReference type="EC" id="2.3.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.13" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673BRD5"/>
    <dbReference type="Ensembl" id="ENSSORT00005046511.1">
        <property type="protein sequence ID" value="ENSSORP00005045361.1"/>
        <property type="gene ID" value="ENSSORG00005020113.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRD5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072378">
        <property type="term" value="P:blood coagulation, fibrin clot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002275">
        <property type="entry name" value="Coagulation factor XIII, A1 polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.260.10:FF:000001">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000171">
        <property type="entry name" value="protein-glutamine gamma-glutamyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF42">
        <property type="entry name" value="COAGULATION FACTOR XIII A CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00927">
        <property type="entry name" value="Transglut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00868">
        <property type="entry name" value="Transglut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000459">
        <property type="entry name" value="TGM_EBP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
        <property type="entry name" value="Transglutaminase, two C-terminal domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="11">
        <location>
            <begin position="325"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="749" mass="84269" checksum="4C63900530A4B772" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTN3</accession>
    <name>A0A673BTN3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BTN3"/>
    <dbReference type="FunCoup" id="A0A673BTN3">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045642.1">
        <property type="protein sequence ID" value="ENSSORP00005044519.1"/>
        <property type="gene ID" value="ENSSORG00005020492.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BTN3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000203">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF52">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY POLYPEPTIDE B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="536" mass="60768" checksum="8FDC1DA645BC121D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BU98</accession>
    <name>A0A673BU98_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Receptor-interacting serine/threonine-protein kinase 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ripk3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BU98"/>
    <dbReference type="Ensembl" id="ENSSORT00005045287.1">
        <property type="protein sequence ID" value="ENSSORP00005044167.1"/>
        <property type="gene ID" value="ENSSORG00005020356.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BU98"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF297">
        <property type="entry name" value="RECEPTOR-INTERACTING SERINE_THREONINE-PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="361"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BV33</accession>
    <name>A0A673BV33_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="20" type="primary">sptlc3</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="20" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
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    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:ceramide biosynthetic process"/>
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        <property type="entry name" value="2-amino-3-ketobutyrate coenzyme A ligase"/>
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        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Pfam" id="PF00155"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="549" mass="61708" checksum="CF44C2A495FE7889" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BV81</accession>
    <name>A0A673BV81_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Nance-Horan syndrome a (congenital cataracts and dental anomalies)</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">nhsa</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1388"/>
            <end position="1398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1466" mass="158940" checksum="F1CCF1A2DDBD2342" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BY34</accession>
    <name>A0A673BY34_9TELE</name>
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        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mfap3l</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030018157.1">
        <property type="nucleotide sequence ID" value="XM_030162297.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030018158.1">
        <property type="nucleotide sequence ID" value="XM_030162298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BY34"/>
    <dbReference type="FunCoup" id="A0A673BY34">
        <property type="interactions" value="753"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047104.1">
        <property type="protein sequence ID" value="ENSSORP00005045949.1"/>
        <property type="gene ID" value="ENSSORG00005021068.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438590"/>
    <dbReference type="CTD" id="9848"/>
    <dbReference type="InParanoid" id="A0A673BY34"/>
    <dbReference type="OrthoDB" id="8611351at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340:SF2">
        <property type="entry name" value="MICROFIBRILLAR-ASSOCIATED PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="61"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="369"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
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        <location>
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            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="421" mass="46788" checksum="5A027FA2CD2B8BB8" modified="2020-06-17" version="1">MTPSLPPWVIHQHLEKMHSVGGLAFVVLVSLFTSQTAGALVSDTVRNHTENGTVTDDGFVPVVFTKVSQIIAREGSCALIDCNVTGDPFPSVQWFNSHGDRLDTETNGGKWWLLENGVLNITSIEFADRGKYTCMASNVHGTSNCTVTLRVVFTNGDMGVYYMVVCLVTFTIIMALNVTRLCMMSSHLKKTEKAINEFFRTEGAEKLQKAFEIAKRIPIITSAKTLELAKVTQFKTMEFARYIEELARSIPLPPLIMNCRTFMEEILEVVGVEEMRHTFVRQAPEGRREVAGQVAAIGARDVFTILQERERERERNRDRERERSDSPTADSDNSSVHEQPQHIAIQVSVHPPLAIGGYCNIEAPNQQEAITSSPPLPALAPLNHEEQSEPKGEQTIEPAAAEQPAANKTPPPCQVFYESHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYB8</accession>
    <name>A0A673BYB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thrombomodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115414213</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway. Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition. Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation. Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with ITGAL, ITGAM and ITGB2. Interacts with thrombin/F2; this interaction switches the specificity of thrombin from a procoagulant to an anticoagulant and antifibrinolytic protease. Interacts with ANGP1 and ANGP2; these interactions significantly inhibit the generation of activated PC and TAFIa/CPB2 by the thrombin/thrombomodulin complex. Interacts with PF4; this interaction enhances generation of activated protein C. Interacts with HMGB1; this interaction inhibits HMGB1 inflammatory activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029983311.1">
        <property type="nucleotide sequence ID" value="XM_030127451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BYB8"/>
    <dbReference type="Ensembl" id="ENSSORT00005049192.1">
        <property type="protein sequence ID" value="ENSSORP00005048006.1"/>
        <property type="gene ID" value="ENSSORG00005021917.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414213"/>
    <dbReference type="InParanoid" id="A0A673BYB8"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015149">
        <property type="entry name" value="Tme5_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789:SF9">
        <property type="entry name" value="THROMBOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09064">
        <property type="entry name" value="EGF_Tme5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025505322" description="Thrombomodulin" evidence="19">
        <location>
            <begin position="35"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="44"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="383" mass="42540" checksum="3D63F7CB70F9A50E" modified="2020-06-17" version="1">MACFHLTVSSHLSKVTSFILLIFLLAMEGGLCMAQTGTCRPICSGGDCVTINQHRVDFKSAEEACRNRNGELLTFQSDTNEIFLEIFSQGVYGNFWIGLQLPAGSCSDISAPLRGYEWTSGSRNHIPLFNNWKDTIKVCSPHCVSVSNDQKWTERPCTDEIEGFLCKTDHKDACQAEDLSDSVMFRSSKGCSDGPCEHDCKPVKDGYRCTCFKGYIPDSKNPRRCKMHCGQMKCPAVCEGNFESCDCPDGYIISEGFCEDMDECEERGLCEENCINTFGSYFCSCKQGFIVKDNGKCIKVADSEHFMTTTIPVITGLAKPASKNNTVTGSSAAAGGFIWLWIFIAVALVVFICVVRYYVVKRQKLREQNSSQQTQAHVDNIEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYY1</accession>
    <name>A0A673BYY1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Gamma-aminobutyric acid (GABA) A receptor, alpha 6a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">gabra6a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BYY1"/>
    <dbReference type="FunCoup" id="A0A673BYY1">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046487.1">
        <property type="protein sequence ID" value="ENSSORP00005045338.1"/>
        <property type="gene ID" value="ENSSORG00005020810.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BYY1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19052">
        <property type="entry name" value="LGIC_TM_GABAAR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000001">
        <property type="entry name" value="Gamma-aminobutyric acid A receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047024">
        <property type="entry name" value="Gabra-1-6_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="28"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="242"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="432" mass="48749" checksum="1790FC0F6896C287" modified="2020-06-17" version="1">KDLLHGLVPGPPWGFAGDDSKINSDDITRILDRLLDGYDNRLRPGSGGGVTEVKTDIFVTSFGPVSDVEMEYTMDMFFRQTWVDERLKFEGPTEILRLNNLMVDKIWTPDTFFRNSKKSISHNMTTPNKLFRIMKNGTVLYTMRLTISAECPMRLMDFPMDGHACPLRFGSYAYTSSEIMFTWKKGPVASVDCPKESMSLLQYDLVGQTLSSEIFKANTGHYSVQVVHFHLQRKLGYYLIQTYIPLIMVVVLSQVSFWINKESVPARTVAGITTVLTMTTLSISARQSLPKVSYATAMDWFIAVCFAFVASALVEFAAVNYFATLQANRLKKQKARQDKLEVLATGSDDDDTVSVSLKRRNHSVCRSEPDDAPPLPIFLQQGSAFPQIPQLAGTSPIDKYARILFPLAFALFNLVYWYIYLAKDTMEQASEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZQ5</accession>
    <name>A0A673BZQ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adrenomedullin 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenomedullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BZQ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005047326.1">
        <property type="protein sequence ID" value="ENSSORP00005046167.1"/>
        <property type="gene ID" value="ENSSORG00005021160.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BZQ5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010460">
        <property type="term" value="P:positive regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003073">
        <property type="term" value="P:regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051665">
        <property type="entry name" value="Adrenomedullin-reg_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414">
        <property type="entry name" value="ADRENOMEDULLIN, ADM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414:SF2">
        <property type="entry name" value="PROTEIN ADM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="162" mass="18397" checksum="CC42F78A5DECE2CB" modified="2020-06-17" version="1">IHIPLRCVCLELILTFFFPFLFLCHIRSSLPKSSKYPKSTLTPIMPTASDLSFTLENRISHGNRQIIWRALIHKEPPQRWPDPLSDQTRGELQEAPVWQRGSRGRRHANNGGSRGHGHLMRVGCVLGTCQVQNLSHRLYQLIGQSGREDSSPINPRSPHSYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C100</accession>
    <name>A0A673C100_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Si:dkey-28o19.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Postsynaptic cell membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the LRRTM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C100"/>
    <dbReference type="FunCoup" id="A0A673C100">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048870.1">
        <property type="protein sequence ID" value="ENSSORP00005047690.1"/>
        <property type="gene ID" value="ENSSORG00005021790.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C100"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000005">
        <property type="entry name" value="leucine-rich repeat transmembrane neuronal protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF283">
        <property type="entry name" value="LEUCINE-RICH REPEAT TRANSMEMBRANE NEURONAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025567214" evidence="14">
        <location>
            <begin position="23"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="371"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="615" mass="68719" checksum="91D4646BF018C1F1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C127</accession>
    <name>A0A673C127_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="8">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">acss1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:23177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>propanoate + ATP + CoA = propanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20373"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:20374"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673C127"/>
    <dbReference type="FunCoup" id="A0A673C127">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047789.1">
        <property type="protein sequence ID" value="ENSSORP00005046627.1"/>
        <property type="gene ID" value="ENSSORG00005021017.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C127"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050218">
        <property type="term" value="F:propionate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000011">
        <property type="entry name" value="Acetyl-coenzyme A synthetase 2-like, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF110">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 2-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="12">
        <location>
            <begin position="60"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="10">
        <location>
            <begin position="115"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="11">
        <location>
            <begin position="565"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="689" mass="76124" checksum="DFDC5173EA72D5A3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2L1</accession>
    <name>A0A673C2L1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Neuropilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">nrp1a</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the neuropilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C2L1"/>
    <dbReference type="FunCoup" id="A0A673C2L1">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049425.1">
        <property type="protein sequence ID" value="ENSSORP00005048235.1"/>
        <property type="gene ID" value="ENSSORG00005021615.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C2L1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038085">
        <property type="term" value="F:vascular endothelial growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005021">
        <property type="term" value="F:vascular endothelial growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010595">
        <property type="term" value="P:positive regulation of endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051491">
        <property type="term" value="P:positive regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030947">
        <property type="term" value="P:regulation of vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000013">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000010">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014648">
        <property type="entry name" value="Neuropilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022579">
        <property type="entry name" value="Neuropilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF4">
        <property type="entry name" value="NEUROPILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11980">
        <property type="entry name" value="DUF3481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036960">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="11 20 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 20" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10 20" id="KW-0221">Differentiation</keyword>
    <keyword evidence="16 22 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="5" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="15 20 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="17 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="861"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="26">
        <location>
            <begin position="59"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="26">
        <location>
            <begin position="179"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="27">
        <location>
            <begin position="295"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="27">
        <location>
            <begin position="451"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="28">
        <location>
            <begin position="687"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="609"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="612"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="22 23">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="295"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="451"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR036960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR036960-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR036960-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="927" mass="103101" checksum="48A9E685BD561A2F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C369</accession>
    <name>A0A673C369_9TELE</name>
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        <submittedName>
            <fullName evidence="7">Oligodendrocyte myelin glycoprotein b</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="primary">omg</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="interactions" value="478"/>
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        <property type="protein sequence ID" value="ENSSORP00005046763.1"/>
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    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:copper ion binding"/>
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        <property type="term" value="F:cytochrome-c oxidase activity"/>
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        <property type="term" value="P:neuron projection regeneration"/>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CcO_II-like_C"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
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        <property type="entry name" value="LRR-bact_E3_ubiq_ligases"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="LRRNT"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47114:SF4">
        <property type="entry name" value="OLIGODENDROCYTE MYELIN GLYCOPROTEIN B"/>
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    </dbReference>
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        <property type="entry name" value="LRR_8"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
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        <location>
            <begin position="28"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="6">
        <location>
            <begin position="117"/>
            <end position="252"/>
        </location>
    </feature>
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        <location>
            <begin position="345"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="390" mass="43252" checksum="E61EB04445C38433" modified="2020-06-17" version="1">MAARPASALCLLLPLLLPLLLLWGLLGGQVLSICPAVCSCSRGHRVVDCSSRGLTKLPPGLQHNIRFLNLSFNSLQDVDAQLSHYAHLRTLDMSYNRLETLPPALPRSLWDIRATGNLLRSLDKNDTAYHWNLKFLDLSDNELERIVFINNTLPGLQTLNLSHNRFWTVPTNMPHNLQSVDLSHNYLVQILPGSLDRLPKLTQFYLHANRFSWLSEGVFDKLSGLQLMTLGDNPWACEEEENITKLLQWAEQTRATVRGCPCYTRPVCGQAHLAAPGGEWHSALFTEPPLWVDGGGEGPDRTAEVTSGYQAKSALFETGIYHNSRGGNDSAEPVVFEWTSSSSSVGFTKRTSTTARPRSFHQQTRVATSHNRGRGLLSDTPSTSLTVLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C377</accession>
    <name>A0A673C377_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Acetoacetyl-CoA synthetase</fullName>
            <ecNumber evidence="3 11">6.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">aacs</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Converts acetoacetate to acetoacetyl-CoA in the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>acetoacetate + ATP + CoA = acetoacetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16117"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.16" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_029999309.1">
        <property type="nucleotide sequence ID" value="XM_030143449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C377"/>
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        <property type="interactions" value="289"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005049791.1"/>
        <property type="gene ID" value="ENSSORG00005022511.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425730"/>
    <dbReference type="CTD" id="65985"/>
    <dbReference type="InParanoid" id="A0A673C377"/>
    <dbReference type="OrthoDB" id="10253869at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030729">
        <property type="term" value="F:acetoacetate-CoA ligase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032024">
        <property type="term" value="P:positive regulation of insulin secretion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="N-terminal domain of ligase-like"/>
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        <property type="entry name" value="Acac_CoA_synth"/>
    </dbReference>
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        <property type="entry name" value="ACAS_N"/>
    </dbReference>
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        <property type="entry name" value="AMP-bd_C_sf"/>
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        <property type="entry name" value="AMP-binding_CS"/>
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        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
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        <property type="entry name" value="ANL_N_sf"/>
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    <dbReference type="NCBIfam" id="TIGR01217">
        <property type="entry name" value="ac_ac_CoA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK03584.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42921">
        <property type="entry name" value="ACETOACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42921:SF1">
        <property type="entry name" value="ACETOACETYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="13">
        <location>
            <begin position="49"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="12">
        <location>
            <begin position="113"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="676" mass="75127" checksum="F616F90E8A285EB5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4F8</accession>
    <name>A0A673C4F8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Si:ch211-265g22.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETS family.</text>
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        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ETS_fam"/>
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    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF306">
        <property type="entry name" value="ETS TRANSLOCATION VARIANT 3-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="8">
        <location>
            <begin position="24"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="198" mass="23298" checksum="B5F3E36B1566E507" modified="2020-06-17" version="1">MYVVTVLFPDWAYKPAWSPGSRQVQLWHFLLELLGRGEGGAIGWGGEWGEFVIRDPERLAKLWGERKGKPHMNYDKLSRALRYYYNKRILHKTKGKRFTYKFNFNKLILVNYPGYPPPGHQVITTSLYLTYSLIGLRTDFDEIFRKCGYWHKQQTRKALGECRPTPRQICPPPSPPNFNHFFLVPVSTFSENLMKIRL</sequence>
</entry>
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        <name evidence="7" type="primary">efhc2</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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            <location evidence="1">Cilium axoneme</location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051406.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="704" mass="80350" checksum="2FF147C41DDE14A2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C851</accession>
    <name>A0A673C851_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Isovaleryl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="6">1.3.8.1</ecNumber>
            <ecNumber evidence="5">1.3.8.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Butyryl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC115413746</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. To a lesser extent, is also able to catalyze the oxidation of other saturated short-chain acyl-CoA thioesters as pentanoyl-CoA, hexenoyl-CoA and butenoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 3-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:12276"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57389"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21 22">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="6"/>
    <dbReference type="EC" id="1.3.8.4" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673C851"/>
    <dbReference type="Ensembl" id="ENSSORT00005049560.1">
        <property type="protein sequence ID" value="ENSSORP00005048368.1"/>
        <property type="gene ID" value="ENSSORG00005021576.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C851"/>
    <dbReference type="UniPathway" id="UPA00363">
        <property type="reaction ID" value="UER00860"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01156">
        <property type="entry name" value="IVD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000003">
        <property type="entry name" value="isovaleryl-CoA dehydrogenase, mitochondrial"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000004">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034183">
        <property type="entry name" value="IVD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21 22" id="KW-0274">FAD</keyword>
    <keyword evidence="8 22" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="25">
        <location>
            <begin position="39"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="24">
        <location>
            <begin position="157"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="23">
        <location>
            <begin position="270"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="281"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
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        <location>
            <begin position="377"/>
            <end position="381"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="404"/>
            <end position="405"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <begin position="406"/>
            <end position="408"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012044"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
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            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031895"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045583"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047736"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00048345"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048375"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-1"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-2"/>
        </source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR634183-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="423" mass="46638" checksum="E71B73FE6D0B4DAA" modified="2020-06-17" version="1">MFAVRNALRLGSRLSISRLSRRGCAGASIPVDDVVNGLTDDQIQLRESVRKFCAEQLAPHADEIDKKNEFPEMRDFWKAMGEMGLLGITAPVEFGGTGMGYLEHVIVMEEISRVSAAIALSYGAHSNLCVNQMVRHGNEEQKEKYLPKLMTGEHVGALAMSEPNAGSDVVSMKLKAVKEGNYYVLNGNKFWITNGPDADVLIVYAKTDPDAHQRGITSVSEQRWMFILQGMPGFSTAQKLDKLGMRGSNTCELIFEDCKVPAKNILGPLNKGVYVMMSGLDLERLVLASGPIGIMQAVLDFAIPYLHVREAFGQKIGHFQLMQGKMADMYTRLSSCRQYLYNVARACDKGHFSAKDCAGVILYCAENATQVALDGIQCLGGNGYINDYPMGRFLRDAKLYEIGAGTSEVRRLIIGRAFNSMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9E0</accession>
    <name>A0A673C9E0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C-factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">zgc:110339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C9E0"/>
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        <property type="protein sequence ID" value="ENSSORP00005049397.1"/>
        <property type="gene ID" value="ENSSORG00005022437.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C9E0"/>
    <dbReference type="OrthoDB" id="7289984at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF20">
        <property type="entry name" value="C-FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="255" mass="27011" checksum="AE8436A828A16894" modified="2020-06-17" version="1">MTGAWTKCKSVLVTGSSRGIGLQLVKELAERSDRPARIIATARDPASSTALQEISKAYQGVHMVTLDVNSQESISSAVQEVQSIVGSEGLSCLINNAAINISTDLNTVTPDAMMTTFQVNSVSPLFVTKAFLPLLQTSASQSTGMGLHRAAVINISSILGSIQANWGETAAFKAYAYRTSKAALNMLTRCLAVDLGPKGIVCMSLHPGWVKTDMGGPLAELTVEESVSGMLSVLSTLSEDDNGGFKDYRGHTLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9K5</accession>
    <name>A0A673C9K5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein kinase C and casein kinase substrate in neurons protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115410804</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. May form heterooligomers with other PACSINs. Interacts (via SH3 domain) with DNM1, SYNJ1 and WASL. Interacts with TRPV4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029978481.1">
        <property type="nucleotide sequence ID" value="XM_030122621.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029978482.1">
        <property type="nucleotide sequence ID" value="XM_030122622.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029978483.1">
        <property type="nucleotide sequence ID" value="XM_030122623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C9K5"/>
    <dbReference type="Ensembl" id="ENSSORT00005050045.1">
        <property type="protein sequence ID" value="ENSSORP00005048853.1"/>
        <property type="gene ID" value="ENSSORG00005022229.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410804"/>
    <dbReference type="InParanoid" id="A0A673C9K5"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07655">
        <property type="entry name" value="F-BAR_PACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000014">
        <property type="entry name" value="Kinase C and casein kinase substrate in neurons protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000009">
        <property type="entry name" value="Protein kinase C and casein kinase substrate in neurons 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF50">
        <property type="entry name" value="PROTEIN KINASE C AND CASEIN KINASE SUBSTRATE IN NEURONS 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="15">
        <location>
            <begin position="10"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="327"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="170"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="387" mass="45213" checksum="62F939AA9D3668D0" modified="2020-06-17" version="1">MASLPKEPPEDAKRQSFWMPGNYVRTVHRTDQSHQACNDIVACFLERAKVEKQYAQQLSQWSSKWKTIVDSRPLYGSLMKAWQCFFTSTERLSALHSSISQSLIEEEGERVRTWQKETFPKKLFCGFKESYDNNLSFSRAQKPWSKKLTKLEKARATFHKACQKEQAAIDKERQANENTEMSPEKKQKFTDAKEKLTEEKEKTRARYEKVLEDVTSYTPRYMEEMEAIFDQSQEEERKRISFLKQVFLSIHRHLDITNNESVKAVYSELHNTLMSISDQDDLKWWKNSHGPGMPTDWPKIEEWVPPVKKLKRKKRDQKGKESRPVMIGGVKVRALYDYTGEEGDELSFKAGEVFLKVEEEDDQGWCRGVLSGGKEGFYPANYVEAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9U5</accession>
    <name>A0A673C9U5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A673C9U5"/>
    <dbReference type="Ensembl" id="ENSSORT00005050759.1">
        <property type="protein sequence ID" value="ENSSORP00005049557.1"/>
        <property type="gene ID" value="ENSSORG00005022490.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C9U5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050868">
        <property type="term" value="P:negative regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047170">
        <property type="entry name" value="PTN12/18/22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45983">
        <property type="entry name" value="TYROSINE PHOSPHATSE N18, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45983:SF1">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="72"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="185" mass="21748" checksum="21325BBAF90FF150" modified="2020-06-17" version="1">VGLCSFFFVQKKCECYWPQKQEQPFVCEPFMLSKYACSVIVHSYLYFLQCSRTLKQLHYVNWPDHGVPDSIPPILDMLQEMRSYQAHDDVPICIHCSAGCGRTGALCVIDYTWNLLKKQMIPPDFSIFDLVQNMRTQRPSVVQTKFFFFFFSLHLTFLKGFIPIYYNIILCILQFFSGNHVFSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBP0</accession>
    <name>A0A673CBP0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LRRC8 pannexin-like TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBP0"/>
    <dbReference type="Ensembl" id="ENSSORT00005054235.1">
        <property type="protein sequence ID" value="ENSSORP00005052981.1"/>
        <property type="gene ID" value="ENSSORG00005023869.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBP0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021040">
        <property type="entry name" value="LRRC8_Pannexin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12534">
        <property type="entry name" value="Pannexin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="LRRC8 pannexin-like TM region" evidence="9">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="96" mass="11249" checksum="CB372BC79DAEAB0D" modified="2020-06-17" version="1">MFSLSELAPLNQHQHRSKLLKPWWEVFMDYLVVLMLMVSVLACTEQLSRDRVVSCFQVILNFSRSSVSQSVSQSVSQSDRQTDRQTDRQTDRQIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGA3</accession>
    <name>A0A673CGA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase ZFP91</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase ZFP91</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Zinc finger protein 91 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non-canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with MAP3K14/NIK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673CGA3"/>
    <dbReference type="Ensembl" id="ENSSORT00005054419.1">
        <property type="protein sequence ID" value="ENSSORP00005053165.1"/>
        <property type="gene ID" value="ENSSORG00005023921.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000183">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZFP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000356">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZFP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF11">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZFP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="141"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="305" mass="35405" checksum="9931A9BC44B91359" modified="2020-06-17" version="1">MCHLTLCRIRECLFNFSDLCFFFSGAEEDEGLSSDEDVPFRDDLNDQSYDPKAERFVCTRGRVGLHFCYCCCIIHRVQLLNRFFWRGRRKKDDKTPRLPKRRKKPPVQYVRCEMEGCGTVLAHPRYLQHHIKYQHLLKKKYVCPHPSCGRLFRLQKQLLRHAKHHTDQRDYICEFCARAFKSSHNLAVHRMIHTGEKPLQCEICGFTCRQKASLNWHMKKHDADATYQFSCSICGKKFEKKDCVVAHKAKSHPEVLIAEALAANAGALITTPASLLELQGNPLLPLYISHVNLISLLCECVLMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CHM3</accession>
    <name>A0A673CHM3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">actc1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the actin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CHM3"/>
    <dbReference type="Ensembl" id="ENSSORT00005054949.1">
        <property type="protein sequence ID" value="ENSSORP00005053685.1"/>
        <property type="gene ID" value="ENSSORG00005024125.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CHM3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0558">Oxidation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="384" mass="42814" checksum="CFED7BA3AFA0C87E" modified="2020-06-17" version="1">MFCRASIMCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CI16</accession>
    <name>A0A673CI16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Solute carrier family 25 member 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">slc25a35</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030010522.1">
        <property type="nucleotide sequence ID" value="XM_030154662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CI16"/>
    <dbReference type="FunCoup" id="A0A673CI16">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053547.1">
        <property type="protein sequence ID" value="ENSSORP00005052302.1"/>
        <property type="gene ID" value="ENSSORG00005023580.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433320"/>
    <dbReference type="CTD" id="399512"/>
    <dbReference type="InParanoid" id="A0A673CI16"/>
    <dbReference type="OrthoDB" id="6703404at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000039">
        <property type="entry name" value="Solute carrier family 25 member 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051508">
        <property type="entry name" value="Mito_Carrier_Antiporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928">
        <property type="entry name" value="RE38146P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928:SF2">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="99"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="202"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="32420" checksum="9C24282FF4570497" modified="2020-06-17" version="1">MDFVLSGAAACGACLFTNPLEVVKTRMQLQGELQSRGTYQVYYRNVFHAFYTIGKVDGLAGLQKGLVPGLVYQFCMNGVRLGSYAIIESSGYIHTNGRVSAVKTTLAGALAGVVGAVMGSPVYLVKTHLQSQSTSSIAVGHQYKHQGMVHALAAIYKDHGIMGLWRGSSAAVPRVSVGSATQLCTFSSSKELVVDLQLFPKDSWQVALSAGMISSVVVVLAMTPFDVVSTRLYNQPVDHLGRGQLYKGFADCFSKTLKKEGVMGLYKGIGASYFRLGPHTILSLFFWDELRKFYQQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CIG3</accession>
    <name>A0A673CIG3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ras homolog family member G</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CIG3"/>
    <dbReference type="Ensembl" id="ENSSORT00005055245.1">
        <property type="protein sequence ID" value="ENSSORP00005053975.1"/>
        <property type="gene ID" value="ENSSORG00005024229.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CIG3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13478" checksum="DF02E500AF6FBE32" modified="2020-06-17" version="1">MADIVIICFSISDPSSYENVKQRWEPEIKHYCPNVPVLLIGTKSDLRDNQEVLEKLRDQNQTTVTQDEGVAMAKKIKAVKYLECASINQEGLEEVFDEAAHAYLRHQQNTTKKICVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMI5</accession>
    <name>A0A673CMI5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NK2 homeobox 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nkx2.9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NK-2 homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CMI5"/>
    <dbReference type="Ensembl" id="ENSSORT00005056697.1">
        <property type="protein sequence ID" value="ENSSORP00005055415.1"/>
        <property type="gene ID" value="ENSSORG00005024729.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CMI5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000101">
        <property type="entry name" value="NK2 homeobox 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF24">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX-2.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055415.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="271" mass="28914" checksum="5406332760FC7255" modified="2020-06-17" version="1">MAAAPNRVSFSVRSILDLPERDAEEAGPHTGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGPGCCPCPLSNLDVSLPASDDGGLEASPDSTKPDDSSLDSEPEKNQKSKKRRVLFSKAQTLELERRFRQQRYLSGPEREQLARLLSLTPTQVKIWFQNHRYKMKRGRAEGAQDAADMGAPPPPLLRRVVVPILVRDGKPFHTCILDTEKAGCLAPSPAVPFPLAYPSVQHPAAALPPRYQHFPGGPAASRYSFTWRDFWSDSVQFGSFR</sequence>
</entry>
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    <accession>A0A673CR21</accession>
    <name>A0A673CR21_9TELE</name>
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        <submittedName>
            <fullName evidence="11">Ubiquitin-associated protein 2-like</fullName>
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    </protein>
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        <name evidence="11" type="primary">LOC115425404</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00165"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055799.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1186" mass="122539" checksum="B9811695A4AFB07E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="21">Paralemmin-1</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
    </protein>
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        <name evidence="24" type="primary">palm1b</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogoninae</taxon>
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    </organism>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="20">Interacts with dopamine receptor DRD3.</text>
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            <topology evidence="18">Lipid-anchor</topology>
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            <location evidence="3">Axon</location>
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            <location evidence="1">Dendrite</location>
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        </subcellularLocation>
    </comment>
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        <text evidence="6">Belongs to the paralemmin family.</text>
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        <property type="component" value="Chromosome 22"/>
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    <dbReference type="GO" id="GO:0016327">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Paralemmin"/>
    </dbReference>
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        <property type="entry name" value="PARALEMMIN-1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498">
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    </dbReference>
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        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0636">Prenylation</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="182"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00040790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="25215" checksum="B76ACACE32818827" modified="2020-06-17" version="1">MAEVSQEERLQAIAEKRKKQTEIENKRRQLDDDRRQLQHLKSKALRERWLLDGAPEEEETQKRLQEDEVKTKLLEQVILRLEQEIEELETGVPANKGVAKENGGENGVVQTSGQTPKREVAGIEAKLLGPSPDQASADNPVTLVFMGYKTVEEEAETRTALGMEGVDGNVKAEFVVIEDGEGKVAGEAATEEQAPPNGSMAEKEKANGGEEGEKEKEKKQTCKCCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CWS4</accession>
    <name>A0A673CWS4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bucky ball</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CWS4"/>
    <dbReference type="FunCoup" id="A0A673CWS4">
        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059376.1">
        <property type="protein sequence ID" value="ENSSORP00005058054.1"/>
        <property type="gene ID" value="ENSSORG00005025662.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CWS4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053309">
        <property type="entry name" value="Balbiani_Body_Formation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38654:SF1">
        <property type="entry name" value="BUCKY BALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38654">
        <property type="entry name" value="BUCKY BALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="64359" checksum="F012EC567BC739A4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CX11</accession>
    <name>A0A673CX11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alpha/beta-tubulin-N-acetyltransferase 9</fullName>
            <ecNumber evidence="6">2.3.1.308</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nat9</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">N-acetyltransferase that mediates the acetylation of the N-terminal residues of alpha- and beta-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-terminal L-methionyl-[tubulin] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-[tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69607"/>
            <dbReference type="Rhea" id="RHEA-COMP:17729"/>
            <dbReference type="Rhea" id="RHEA-COMP:17730"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="ChEBI" id="CHEBI:133414"/>
            <dbReference type="EC" id="2.3.1.308"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.308" evidence="6"/>
    <dbReference type="RefSeq" id="XP_029977764.1">
        <property type="nucleotide sequence ID" value="XM_030121904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CX11"/>
    <dbReference type="Ensembl" id="ENSSORT00005058582.1">
        <property type="protein sequence ID" value="ENSSORP00005057267.1"/>
        <property type="gene ID" value="ENSSORG00005025414.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410303"/>
    <dbReference type="CTD" id="26151"/>
    <dbReference type="InParanoid" id="A0A673CX11"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120519">
        <property type="term" value="F:tubulin N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000040">
        <property type="entry name" value="N-acetyltransferase 9 (putative)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039135">
        <property type="entry name" value="NAT9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256:SF16">
        <property type="entry name" value="ALPHA_BETA-TUBULIN-N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256">
        <property type="entry name" value="N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="8">
        <location>
            <begin position="13"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00058998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="210" mass="24349" checksum="B95F7AF741164FD5" modified="2020-06-17" version="1">MKINENTLLEGQRVVLVPYNAEHVPRYHEWMKSAELQQLTASEPLTLEQEYDMQKSWREDDDKCTFIILDKQQWANPEVGEEQCMVGDVNIFLTDPTDLSLAELEVMIAEPSFRGKGIGKEVTLMMMHYGVSKLGVQKFEVKIGLDNQISIAMFKKLQFQEVSVCKVFKEMTLQLPVVESVRTTLLHQTSHMKEWDYKQSCSNRQEMTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CYJ4</accession>
    <name>A0A673CYJ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">snx1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CYJ4"/>
    <dbReference type="Ensembl" id="ENSSORT00005059258.1">
        <property type="protein sequence ID" value="ENSSORP00005057937.1"/>
        <property type="gene ID" value="ENSSORG00005025613.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CYJ4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Sorting nexin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000012">
        <property type="entry name" value="Sorting nexin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005329">
        <property type="entry name" value="Sorting_nexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF129">
        <property type="entry name" value="SORTING NEXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03700">
        <property type="entry name" value="Sorting_nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="16">
        <location>
            <begin position="158"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="65"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="556" mass="63290" checksum="C92FB2B40C1821FF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y701</accession>
    <name>A0A672Y701_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SLIT and NTRK like family member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLITRK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y701"/>
    <dbReference type="Ensembl" id="ENSSORT00005002235.1">
        <property type="protein sequence ID" value="ENSSORP00005002178.1"/>
        <property type="gene ID" value="ENSSORG00005001323.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y701"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000001">
        <property type="entry name" value="SLIT and NTRK-like family, member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773:SF5">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025357107" evidence="11">
        <location>
            <begin position="40"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="645"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="12">
        <location>
            <begin position="234"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="12">
        <location>
            <begin position="556"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="754"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="763"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="890" mass="100781" checksum="06B989AA238FC3C9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8K6</accession>
    <name>A0A672Y8K6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y8K6"/>
    <dbReference type="Ensembl" id="ENSSORT00005000104.1">
        <property type="protein sequence ID" value="ENSSORP00005000089.1"/>
        <property type="gene ID" value="ENSSORG00005000074.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8K6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000005">
        <property type="entry name" value="Neurotrypsin isoform A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF15">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="9"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="121"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="226"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="35"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="48"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="147"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="160"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="252"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="265"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="421" mass="46018" checksum="3CD2C14DA02362A8" modified="2020-06-17" version="1">MLFLIGVQIRLVGSGSTRCSGRVEVYHNGVWGTVCDDDWDFNDTQVVCRQLGCDTRDIVPFTPRFGEGTGQIWLDDVSCLGNESSLTDCQHKGFGNHDCSHTEDVSVACSGKMTFGYSSDIRLAGSGSTQCSGRVQVSQNGTWGTVCDDDWDLNDAQVVCRQLGCGSALSAHPSAHFGQGSGPVWLTDVRCSGHEQYLVQCEHGGLGRRHCGHDKDVGVVCEDVQIKLVGPGSTQCSGRVEVYYNGTWGRVCNLYWGFSDAQVVCRQMGCETAQAVSAAGQFGEGTGPVWLDDVRCSGKESSLTECPHHKSETHDCEYGKDFGVVCSGKKTFCSDIVCFSKPFYQFNNSVIQLTLFISLFIGPSPVLSILCSHKLLPTDDSCQSLYTCSQKSPHQLFPCFLCLRLSSQTGRVWFYSVFWKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCP6</accession>
    <name>A0A672YCP6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sft2d2b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YCP6"/>
    <dbReference type="FunCoup" id="A0A672YCP6">
        <property type="interactions" value="414"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002877.1">
        <property type="protein sequence ID" value="ENSSORP00005002794.1"/>
        <property type="gene ID" value="ENSSORG00005001735.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCP6"/>
    <dbReference type="OrthoDB" id="73614at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF1">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="162" mass="17466" checksum="B5E6C357E264A342" modified="2020-06-17" version="1">MDKLKQVLGGQDGGTTDGTGILERANQASTLAWGTRVKGFVVCFILGVVCSVLGTCLLWIPGAGLPAFAVLYTVGNICSLASTMFLVGPLKQLKSMWAKERALATAVMLVCLVLTLCAAFWWKNNGLALLFCILQFLAFTWYGLSYIPFARDAALRLCSMCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFW4</accession>
    <name>A0A672YFW4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mesothelin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YFW4"/>
    <dbReference type="Ensembl" id="ENSSORT00005003546.1">
        <property type="protein sequence ID" value="ENSSORP00005003446.1"/>
        <property type="gene ID" value="ENSSORG00005002079.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFW4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010335">
        <property type="entry name" value="Mesothelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026664">
        <property type="entry name" value="Stereocilin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412:SF6">
        <property type="entry name" value="MESOTHELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412">
        <property type="entry name" value="STEREOCILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06060">
        <property type="entry name" value="Mesothelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="478" mass="52864" checksum="2016D4457E4F06F3" modified="2020-06-17" version="1">LSINYQLLLMVFKIVKINENSEVIVDNVPDDLASEIPRASLLGFSGTSNFLKINKKKWKRQQVELFFGVVAVENATSAVGGTANLSSSVLQGFTCTAVRTIQEVQIKRLIKACRRKNKVTLVETQVRVLDEDKDEDLFGKLPYSLVPQSKCRSYFEQLSDADFSVFSNVLSYKRTALFDNARSCLGITNTSLTEDNISVLGNMCCTLDGAYIENSDPSILEKLNNCPELSNAQATAVETLLVSGRTKYRAVSTWNEQTLRDLGMLPLYMTSTFYDHFPKVSDTLVSYLKSFVCIIKRVCSSSVLECTVGVITQVTISDETFPFDYDDINQFDSCLSNTTVKNNLAAITDKVDDKDYLRIVLRKLREVASIPEDQVQLLGPAACVATVEDINVWTITTIDTLSALMDPSDGTWDPSLAKAVITKYLSIDGNSLGTAELNALGGVNLCSLDVDVLKTITEQSLKLSFTHVHYNLQITVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YG97</accession>
    <name>A0A672YG97_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zgc:162255</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">c5h6orf89</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YG97"/>
    <dbReference type="FunCoup" id="A0A672YG97">
        <property type="interactions" value="996"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003683.1">
        <property type="protein sequence ID" value="ENSSORP00005003582.1"/>
        <property type="gene ID" value="ENSSORG00005002150.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YG97"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038757">
        <property type="entry name" value="BRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35259">
        <property type="entry name" value="BOMBESIN RECEPTOR-ACTIVATED PROTEIN C6ORF89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35259:SF1">
        <property type="entry name" value="BOMBESIN RECEPTOR-ACTIVATED PROTEIN C6ORF89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="332" mass="37320" checksum="15F4E7F345F39893" modified="2020-06-17" version="1">MGTTTSEPCIYDKLSESVEILRQSGYRYGMSEREIERFIKQVLETNEPRRDPPQFPILHATIKFMLIVGVLLLVLLAFTYPQRTPHLALVNLGSYNWSSPLSHVRLLSLPIAKKYNLQGFHEWWSGGSLQHTPVNCSGCAVITSVLQFPESLRRTVTLRRGPQLVLLKGGSSLSVQRQQLEELYLAHSMSMSIVLEEDGLQTQNSGLPQGPANFTLLWYCFSIGTRGKVLRWLFPKAELCPMLDSSGTNLQRCLVKHTTNAQSKGVRVLGWLVVGEGLPTVRVLPVQRCHKHCSSFNLGLTPGDMVYADPRYWQMELFPGQGQNIICDGSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YH44</accession>
    <name>A0A672YH44_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Synaptotagmin-like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sytl4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YH44"/>
    <dbReference type="Ensembl" id="ENSSORT00005004012.1">
        <property type="protein sequence ID" value="ENSSORP00005003901.1"/>
        <property type="gene ID" value="ENSSORG00005002368.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YH44"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04020">
        <property type="entry name" value="C2B_SLP_1-2-3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15764">
        <property type="entry name" value="FYVE_Slp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000006">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000018">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044134">
        <property type="entry name" value="FYVE_Slp4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043567">
        <property type="entry name" value="SYTL1-5_C2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716:SF4">
        <property type="entry name" value="SYNAPTOTAGMIN-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RabBD" evidence="7">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="386"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="137"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="677" mass="74965" checksum="5BA5BA4101E8F070" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ46</accession>
    <name>A0A672YJ46_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJ46"/>
    <dbReference type="Ensembl" id="ENSSORT00005004756.1">
        <property type="protein sequence ID" value="ENSSORP00005004620.1"/>
        <property type="gene ID" value="ENSSORG00005002811.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJ46"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003074">
        <property type="entry name" value="Immunoglobulin heavy variable 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="94" mass="10639" checksum="D44DF1E8A379FCEA" modified="2020-06-17" version="1">PAVKRPGESHRLTCTTSGFTFSSYWMAWIRQAPGKGLEWIAWISSGSGSTTYYSQSVQGRFTISRDNGRNQLYLQMNSLKTEDSAVYYCARDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YK86</accession>
    <name>A0A672YK86_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">STE20-related kinase adapter protein alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">STE20-related adapter protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">strada</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YK86"/>
    <dbReference type="Ensembl" id="ENSSORT00005005145.1">
        <property type="protein sequence ID" value="ENSSORP00005005001.1"/>
        <property type="gene ID" value="ENSSORG00005002982.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YK86"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000130">
        <property type="entry name" value="STE20-related kinase adapter protein alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
        <property type="entry name" value="STRAD_A/B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE FRAY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF20">
        <property type="entry name" value="STE20-RELATED KINASE ADAPTER PROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="34"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="272"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="368"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="401" mass="44332" checksum="C75F2F1FBD8CF072" modified="2020-06-17" version="1">PSFLLLAHEDSQDSLTSFPHWDTMGSFLPDSSAYELLAVIGRGLDDLMTVNLARYRPTGDHVAIRRIDLESCTNDMVTYLQGELHVSKLFHHSSILPYKSVFIAENELWVITPFMAYGSARDLICTHFTDGMSELTIAYILLGMLRALEYIHHMGYVHRSVKASHVLISADGQVCMSGLRSIFSLIRHGQRAKVVHDFPQYSVKVLPWLSPEVLQQNLHGYDSRSDIYSLGITACELANGHVPFKDMPATQMLLEKLNGTVPCLLDTTTIPPEELSMKPSRSGADSGICEGPGAGGVRHSNGDPSSTSGGHPYNRTFSPHFHAFVELCLQRDPEKRPSASTLIGHPFLKQIKRRPSEALPELLRPVSPITSFEGSQPQDSPSGLASLESGLSHLEVDDWDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YK93</accession>
    <name>A0A672YK93_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transmembrane protein 87B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tmem87b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LU7TM family. TMEM87 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030011674.1">
        <property type="nucleotide sequence ID" value="XM_030155814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YK93"/>
    <dbReference type="Ensembl" id="ENSSORT00005005133.1">
        <property type="protein sequence ID" value="ENSSORP00005004989.1"/>
        <property type="gene ID" value="ENSSORG00005002994.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434117"/>
    <dbReference type="CTD" id="84910"/>
    <dbReference type="InParanoid" id="A0A672YK93"/>
    <dbReference type="OrthoDB" id="19932at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054101">
        <property type="entry name" value="TMEM87A/B_GOLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF16">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 87B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21901">
        <property type="entry name" value="TMEM87A-B_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025352110" description="Transmembrane protein 87B" evidence="10">
        <location>
            <begin position="38"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM87A/B GOLD" evidence="12">
        <location>
            <begin position="38"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="11">
        <location>
            <begin position="233"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="64552" checksum="E08C3B02E895324D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRX1</accession>
    <name>A0A672YRX1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Gastrula zinc finger protein XlCGF57.1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115427901</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YRX1"/>
    <dbReference type="Ensembl" id="ENSSORT00005007616.1">
        <property type="protein sequence ID" value="ENSSORP00005007338.1"/>
        <property type="gene ID" value="ENSSORG00005004198.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007617.1">
        <property type="protein sequence ID" value="ENSSORP00005007339.1"/>
        <property type="gene ID" value="ENSSORG00005004198.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8939517at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="67"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="346" mass="39905" checksum="4463F8169C35F0B4" modified="2020-06-17" version="1">MSHKKQILRSLVEQRLTAAAEEIFGLFERTIAEYEEELCRTKEENQRQKQILDEVWNLKVLTIPAGSSADVQQPSGVGEEVPPEQDDWSFSLDQEEPEPPHIKEEQEELWTNQEADFPNVPPTPMSVKREDDDKEEAQSSLLLLRQCEEKTKERDEEDTGGSGPNFNQSRIRAFNSRSPGSRRSTRSLQKHKDTQTETRLVDSKCLGESNAPRDKLTATNFALSDVRCKSRPFQCSDCGKTYCRRSALNTHRNIHTRERQFPCSVCRKIFGRNSHLRSHMKIHTGEKPYSCPVCQKCFRHKCVMKVHMTTHTGVRAFACSECGRTFTQRPHLIRHMKSHIKKKPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSV4</accession>
    <name>A0A672YSV4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Neurobeachin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Lysosomal-trafficking regulator 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nbeaa</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with RII subunit of PKA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat neurobeachin family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A672YSV4">
        <property type="interactions" value="507"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007967.1">
        <property type="protein sequence ID" value="ENSSORP00005007686.1"/>
        <property type="gene ID" value="ENSSORG00005004332.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YSV4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06071">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01201">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000036">
        <property type="entry name" value="Neurobeachin isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1540.10:FF:000001">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000059">
        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000010">
        <property type="entry name" value="neurobeachin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010508">
        <property type="entry name" value="NBEA-like_DUF1088"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF62">
        <property type="entry name" value="NEUROBEACHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06469">
        <property type="entry name" value="DUF1088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="12">
        <location>
            <begin position="2132"/>
            <end position="2240"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="11">
        <location>
            <begin position="2259"/>
            <end position="2547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="863"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1086"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1120"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1156"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1354"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1458"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1540"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1619"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1643"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1781"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1120"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1157"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1171"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1196"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1459"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1553"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1654"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1786"/>
            <end position="1796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2930" mass="323812" checksum="A7F96EEC61825011" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTC0</accession>
    <name>A0A672YTC0_9TELE</name>
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        <submittedName>
            <fullName evidence="7">Unc-93 homolog B1, TLR signaling regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">unc93b1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YTC0"/>
    <dbReference type="FunCoup" id="A0A672YTC0">
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        <property type="gene ID" value="ENSSORG00005004406.1"/>
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    <dbReference type="InParanoid" id="A0A672YTC0"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:lysosome"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:Toll-like receptor binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:toll-like receptor 3 signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0034154">
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:toll-like receptor signaling pathway"/>
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    </dbReference>
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        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043268">
        <property type="entry name" value="UNC93B1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN UNC-93 HOMOLOG B1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN UNC-93 HOMOLOG B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="318"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="370"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="404"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="429"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
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            <begin position="582"/>
            <end position="592"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="593"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="606" mass="69095" checksum="CB1D0EF7647CC52C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYP7</accession>
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        <submittedName>
            <fullName evidence="17">Histone-lysine N-methyltransferase PRDM9-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="17" type="primary">LOC115439601</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030163442.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010046.1">
        <property type="protein sequence ID" value="ENSSORP00005009720.1"/>
        <property type="gene ID" value="ENSSORG00005005330.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439601"/>
    <dbReference type="CTD" id="56979"/>
    <dbReference type="InParanoid" id="A0A672YYP7"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19193">
        <property type="entry name" value="PR-SET_PRDM7_9"/>
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    </dbReference>
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        <property type="entry name" value="PR domain containing 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044417">
        <property type="entry name" value="PRDM7_9_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 13" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="517"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="667"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="701"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="735"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="784"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="812"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="840"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="868"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="896"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="924"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="369"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="420"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="547"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="948" mass="106820" checksum="A6ADE0CC035A00D4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZR1</accession>
    <name>A0A672YZR1_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115422367</name>
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    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:retinal pigment epithelium development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
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        <location>
            <begin position="36"/>
            <end position="96"/>
        </location>
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        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010291.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="348" mass="37206" checksum="FE3E60925F8FA9EA" modified="2020-06-17" version="1">MMSYLKQAPYGMNGLGLSGAAMDLLHPSVGYPANPRKQRRERTTFTRTQLDILESLFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQSSSSAKVKPMKKKSSPTRESTGSESSGQFTPPAVSSSSSSASSSASSSSSSSSSSSSVSSSLGGPGLISTSTSVTAPVSSIWSPAPVSPALAPATLPDPVAPTSASCMQRSVSSSATGGTPSYPVSYSQTAAAYSQGYPTSASGSYFGGVECGSYLPMHSHHHPHQLSPMTGSSMSGHPHHHIGQTSGHHHHHHHPSHHHQAYSAPGLAFNSTDCLDYKEQTAASAWKLNFNTSDCLDYKDQASWRFQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1A8</accession>
    <name>A0A672Z1A8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z1A8"/>
    <dbReference type="Ensembl" id="ENSSORT00005010831.1">
        <property type="protein sequence ID" value="ENSSORP00005010494.1"/>
        <property type="gene ID" value="ENSSORG00005005668.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1A8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF52">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY POLYPEPTIDE B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025705660" description="UDP-glucuronosyltransferase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="525" mass="60580" checksum="E33C62FE88B51C23" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4J0</accession>
    <name>A0A672Z4J0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Maturin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mturn</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MTURN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004035.1">
        <property type="nucleotide sequence ID" value="XM_030148175.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030004036.1">
        <property type="nucleotide sequence ID" value="XM_030148176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z4J0"/>
    <dbReference type="FunCoup" id="A0A672Z4J0">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012008.1">
        <property type="protein sequence ID" value="ENSSORP00005011623.1"/>
        <property type="gene ID" value="ENSSORG00005006207.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428922"/>
    <dbReference type="CTD" id="222166"/>
    <dbReference type="InParanoid" id="A0A672Z4J0"/>
    <dbReference type="OrthoDB" id="9922400at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045654">
        <property type="term" value="P:positive regulation of megakaryocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027892">
        <property type="entry name" value="Maturin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32008">
        <property type="entry name" value="MATURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32008:SF2">
        <property type="entry name" value="MATURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15167">
        <property type="entry name" value="DUF4581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="14712" checksum="FEC2665CE2FDCF26" modified="2020-06-17" version="1">MEFKHLVEAAEKWCSGNPFDLIFAEEDDERRLDFYAEPGVSFYVLCPGGTDSFHVWSESEDCLPFLQLAQDYISSCGKKTLLEVLEKVFTSFRPLLGLPDIEDDSFEHYHADMEGEPGPDHQQMGVSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7G9</accession>
    <name>A0A672Z7G9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">trypsin</fullName>
            <ecNumber evidence="9">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115428819</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z7G9"/>
    <dbReference type="Ensembl" id="ENSSORT00005012855.1">
        <property type="protein sequence ID" value="ENSSORP00005012458.1"/>
        <property type="gene ID" value="ENSSORG00005006331.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z7G9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF15">
        <property type="entry name" value="RIKEN CDNA 2210010C04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="19"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="248" mass="27628" checksum="A4F5F70FAFBE3664" modified="2020-06-17" version="1">LWILLFYFHIIAASEDDKVVGGYECPRHSVPYQVSLNAGYHFCGGSLISSQWVVSAAHCYKSRIQVRLGEHNIAVNEGTEQWIDGAKMIRHPQYNSYNLDNDIMLIKLSRPATLNSYVQTVSLPSRCPVADENCMVSGWGNTSANGSEFSHRHYNFPDRLQCLRQPIIDDRICRNAYPHLFTNNMVCSGFMAGGASSCQGDSGGPLVCNGQLQGVVSWGYDCAMQGHPSVYARVCRYNSWISSVMRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7Z2</accession>
    <name>A0A672Z7Z2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z7Z2"/>
    <dbReference type="Ensembl" id="ENSSORT00005012994.1">
        <property type="protein sequence ID" value="ENSSORP00005012597.1"/>
        <property type="gene ID" value="ENSSORG00005006594.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z7Z2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF36">
        <property type="entry name" value="RETROTRANSPOSON GAG-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
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        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="682" mass="76556" checksum="0F4E28EC7AA7BAB8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8E3</accession>
    <name>A0A672Z8E3_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tbx3a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z8E3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:cell fate specification"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="T-box transcription factor TBX3"/>
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        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
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        <property type="entry name" value="TBX2_3_RD"/>
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        <property type="entry name" value="TF_Brachyury"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF91">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TBOX"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
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        <location>
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        <location>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="713" mass="77613" checksum="97D5180707997AB3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8Y3</accession>
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        <submittedName>
            <fullName evidence="10">Dedicator of cytokinesis 3</fullName>
        </submittedName>
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        <name evidence="10" type="primary">LOC115420086</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DOCK family.</text>
    </comment>
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        <property type="gene ID" value="ENSSORG00005006774.1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="dedicator of cytokinesis protein 4"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000003">
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        <property type="entry name" value="DOCK_C_lobe_A"/>
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        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF4">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
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    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025503760" evidence="7">
        <location>
            <begin position="18"/>
            <end position="2029"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="8">
        <location>
            <begin position="409"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="9">
        <location>
            <begin position="1216"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1708"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1850"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1708"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
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            <end position="1899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1900"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1975"/>
            <end position="1986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2029" mass="232550" checksum="40E60B8C3DCB0DA9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9W3</accession>
    <name>A0A672Z9W3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein eL33</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">60S ribosomal protein L35a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rpl35a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z9W3"/>
    <dbReference type="FunCoup" id="A0A672Z9W3">
        <property type="interactions" value="948"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013723.1">
        <property type="protein sequence ID" value="ENSSORP00005013313.1"/>
        <property type="gene ID" value="ENSSORG00005006903.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9W3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.190:FF:000005">
        <property type="entry name" value="60S ribosomal protein L35a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_eL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_eL33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="111" mass="12577" checksum="ECF6E627575AD060" modified="2020-06-17" version="1">MTGESRLWSKAIFTGYKRGLRNQREHTALLKIEGCYTRDEIDFYLGKRCAYVYKAKKNTVTPGGKPNKTRVIWGKVTRAHGSSGMVRAKFSSNLPAKAIGHRVRVVYVTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZE38</accession>
    <name>A0A672ZE38_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZE38"/>
    <dbReference type="Ensembl" id="ENSSORT00005016104.1">
        <property type="protein sequence ID" value="ENSSORP00005015611.1"/>
        <property type="gene ID" value="ENSSORG00005007920.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZE38"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001235">
        <property type="entry name" value="Si:ch211-119o8.6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001156">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF114">
        <property type="entry name" value="KLUMPFUSS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="378"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="434"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="462"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="145"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="515" mass="59656" checksum="FA8485FD030022C7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZEX6</accession>
    <name>A0A672ZEX6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Retinoid isomerohydrolase</fullName>
            <ecNumber evidence="16">3.1.1.64</ecNumber>
            <ecNumber evidence="15">5.3.3.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Lutein isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Meso-zeaxanthin isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">rpe65a</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>all-trans-retinyl hexadecanoate + H2O = 11-cis-retinol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31775"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16302"/>
            <dbReference type="ChEBI" id="CHEBI:17616"/>
            <dbReference type="EC" id="3.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an all-trans-retinyl ester + H2O = 11-cis-retinol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31771"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16302"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:63410"/>
            <dbReference type="EC" id="3.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>lutein = (3R,3'S)-zeaxanthin</text>
            <dbReference type="Rhea" id="RHEA:12729"/>
            <dbReference type="ChEBI" id="CHEBI:28838"/>
            <dbReference type="ChEBI" id="CHEBI:138919"/>
            <dbReference type="EC" id="5.3.3.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="21">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.64" evidence="16"/>
    <dbReference type="EC" id="5.3.3.22" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZEX6"/>
    <dbReference type="FunCoup" id="A0A672ZEX6">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015499.1">
        <property type="protein sequence ID" value="ENSSORP00005015038.1"/>
        <property type="gene ID" value="ENSSORG00005007651.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZEX6"/>
    <dbReference type="OrthoDB" id="1069523at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052885">
        <property type="term" value="F:all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003834">
        <property type="term" value="F:beta-carotene 15,15'-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050251">
        <property type="term" value="F:retinol isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042574">
        <property type="term" value="P:retinal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901827">
        <property type="term" value="P:zeaxanthin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF57">
        <property type="entry name" value="RETINOID ISOMEROHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 21" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0844">Vision</keyword>
    <feature type="binding site" evidence="21">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="531" mass="60547" checksum="989D27B327221567" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFL3</accession>
    <name>A0A672ZFL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gar1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the GAR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000967.1">
        <property type="nucleotide sequence ID" value="XM_030145107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZFL3"/>
    <dbReference type="FunCoup" id="A0A672ZFL3">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016113.1">
        <property type="protein sequence ID" value="ENSSORP00005015619.1"/>
        <property type="gene ID" value="ENSSORG00005007926.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426873"/>
    <dbReference type="CTD" id="54433"/>
    <dbReference type="InParanoid" id="A0A672ZFL3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034513">
        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000454">
        <property type="term" value="P:snoRNA guided rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.230:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23237:SF6">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23237">
        <property type="entry name" value="NUCLEOLAR PROTEIN FAMILY A MEMBER 1 SNORNP PROTEIN GAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="149"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="161"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="218" mass="22172" checksum="793EDFB15E6194D3" modified="2020-06-17" version="1">MSYRGGGGRGGGGGFNRGGGGGFNRGGRGGGFGGGRGGGGGFGRGGGRGGFNRQQDYGPPEYVVALGEFMHPCEDDIVCKCTTEENKVPYFNAPVYLENKEQIGKVDEIFGQLRDFYFSVKLSDNMKASSFKKLQKFYIDPMKLLPLQRFLPRPPGEKGPPRGGRGGGRGGGRGGGRGGGFRGGRGGGGRGGGFGGGRGGGGFRGGRGGGGGGRGFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGR0</accession>
    <name>A0A672ZGR0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein phosphatase 1H-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ppm1j</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PPM-type phosphatase domain"/>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 1H"/>
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            <end position="493"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="20"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015668.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="500" mass="55375" checksum="1E23D3C04D58C43C" modified="2020-06-17" version="1">MMISKVKNAMSTLVGGMMPHGHHHHHHNHHSGAGGQTCGPDSLPPRFPYGRPDFLDLTPELLQYSTEHASRPVLTLKRGSRLPWTTGYAEVINAGKSMLNEDQASCEKLFVKKPSGKHRNSTLLEDNGDGTGIPMHFWGVFDGHAGSGAAIMASKLLHRLIRDRLGEICHLLENPTSAPPICLAKNGSPYQAEAKKGATQETEDPDSVSDSSIRFHMEKVVSLESLVMGVIETAFKQMDDLIEKEKASYAISGGCCALAAIHLMGKLYVANAGDSRAIIIRNNEVIPVTNEFTPESERQRLQYLGFLRPELLGNEFTHIEFPRRIQHSELGKKMLYRDHTMTGWAYKTIVEDDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVYNSNIYIKPFLSCVPEVQVYNLDENKHGPNDVLVMGTDGLWDVTTDREVADAVSAYLSCCDPTDPMRYTLAAQDLLMRSRGVLKERGWRLPNDRLGSGDDITVFVIPLAGQESET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI04</accession>
    <name>A0A672ZI04_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZI04"/>
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        <property type="protein sequence ID" value="ENSSORP00005016389.1"/>
        <property type="gene ID" value="ENSSORG00005008318.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="GAG-RELATED PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="190" mass="21711" checksum="2FC48425F7D6295E" modified="2020-06-17" version="1">VDSKTPYGTDIRQEHNDPKWRDWSVESSVQTDVISVVVKFWTGRVPDEDIDLYLRRFCDILHTVKPVDKLGIWYGVRKYKVRLHKNANGHFIQIPNSISLGPYNGRIIYPGQSNTCFICQSTDHQVKDCTQVKCWRCGHLGHKAKDCSSEGRCSLCGVDGHTFFTCPDSYANKTRSQQNPKIGYLLNSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI25</accession>
    <name>A0A672ZI25_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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        <property type="protein sequence ID" value="ENSSORP00005016122.1"/>
        <property type="gene ID" value="ENSSORG00005008155.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="obscurin-like isoform X3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000211">
        <property type="entry name" value="Obscurin-like protein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000502">
        <property type="entry name" value="obscurin-like protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000050">
        <property type="entry name" value="Titin isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000214">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052385">
        <property type="entry name" value="Obscurin/Obscurin-like_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971:SF4">
        <property type="entry name" value="OBSCURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971">
        <property type="entry name" value="SI:DKEY-31G6.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="15"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="14"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="114"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="222"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="314"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="495"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="669"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="745"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="857"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="941"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1021"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1101"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1200"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1289"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="1562"/>
            <end position="1616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1639" mass="181951" checksum="FE21617E11B9D202" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZIM1</accession>
    <name>A0A672ZIM1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115419226</name>
        <name evidence="17" type="synonym">LOC115425062</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989741.1">
        <property type="nucleotide sequence ID" value="XM_030133881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZIM1"/>
    <dbReference type="SMR" id="A0A672ZIM1"/>
    <dbReference type="Ensembl" id="ENSSORT00005016845.1">
        <property type="protein sequence ID" value="ENSSORP00005016337.1"/>
        <property type="gene ID" value="ENSSORG00005008288.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016876.1">
        <property type="protein sequence ID" value="ENSSORP00005016368.1"/>
        <property type="gene ID" value="ENSSORG00005008304.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040707.1">
        <property type="protein sequence ID" value="ENSSORP00005039695.1"/>
        <property type="gene ID" value="ENSSORG00005018521.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419226"/>
    <dbReference type="OrthoDB" id="8735094at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000078">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000044">
        <property type="entry name" value="Histone H3.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="11 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="136" mass="15388" checksum="6FD8508EA50A0EEC" modified="2020-06-17" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKZ8</accession>
    <name>A0A672ZKZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Palmitoyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">zdhhc8b</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZKZ8"/>
    <dbReference type="FunCoup" id="A0A672ZKZ8">
        <property type="interactions" value="652"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017982.1">
        <property type="protein sequence ID" value="ENSSORP00005017464.1"/>
        <property type="gene ID" value="ENSSORG00005008730.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZKZ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF1">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="16">
        <location>
            <begin position="113"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="443"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="539"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="619"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="662"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="733"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="674"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="777" mass="86238" checksum="5C91768AA7737855" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZM66</accession>
    <name>A0A672ZM66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fbxo4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029998942.1">
        <property type="nucleotide sequence ID" value="XM_030143082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZM66"/>
    <dbReference type="Ensembl" id="ENSSORT00005018415.1">
        <property type="protein sequence ID" value="ENSSORP00005017889.1"/>
        <property type="gene ID" value="ENSSORG00005008894.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425471"/>
    <dbReference type="CTD" id="26272"/>
    <dbReference type="InParanoid" id="A0A672ZM66"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22085">
        <property type="entry name" value="F-box_FBXO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11656">
        <property type="entry name" value="FBX4_GTPase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039588">
        <property type="entry name" value="FBXO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16008">
        <property type="entry name" value="F-BOX ONLY PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16008:SF4">
        <property type="entry name" value="F-BOX ONLY PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="45"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="401" mass="45231" checksum="418436180925AB98" modified="2020-06-17" version="1">MAAKIQQLNQSVVIRSLRRFRDRYFPHSGQSVAPVEDTGQEEVTPGFLDNLPVDLQFLVMTFLSPADICCLGATSRYWRGMVRDPLLWRFFLLRDMPHWASIDHLTMPQLEMLDSPLVNEDETLDDREEWEAKENPKPDYMAEYLKGCPSCRQQWLPSKAPYEVVTSFFQSLVPSSEPRYAMFGPGMEQLDVSLVTRIMHAPDVLPVSGTPHRQINGIGSGISYMFNSQHKFNILTLYSTNRAERERARVEKQSISNKLFNLEGTDESGRPVYSPAPHVQQVCQVVDGFIYVANAEHDRDEGGSEAAQIRAVLNPAWGSASRPLLVLSCVSREEWEVLTPHSLHTGGSRNRVPCVHMAKRLGLPQLTNPWMVQDAVAESLSGLLDGISWLLRCSGVKIHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZSD3</accession>
    <name>A0A672ZSD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA replication licensing factor MCM7</fullName>
            <ecNumber evidence="16">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">mcm7</name>
        <name evidence="16" type="synonym">MCM7</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZSD3"/>
    <dbReference type="Ensembl" id="ENSSORT00005020772.1">
        <property type="protein sequence ID" value="ENSSORP00005020205.1"/>
        <property type="gene ID" value="ENSSORG00005009618.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZSD3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17758">
        <property type="entry name" value="MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000004">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000007">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000288">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008050">
        <property type="entry name" value="MCM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF26">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24901">
        <property type="entry name" value="WHD_MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01663">
        <property type="entry name" value="MCMPROTEIN7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 16" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="334"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="729" mass="81936" checksum="46DFC4FAB1A5B8AD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTP3</accession>
    <name>A0A672ZTP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115423073</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZTP3"/>
    <dbReference type="FunCoup" id="A0A672ZTP3">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021099.1">
        <property type="protein sequence ID" value="ENSSORP00005020526.1"/>
        <property type="gene ID" value="ENSSORG00005009990.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZTP3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="50"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="36463" checksum="4B2D80CF58A02572" modified="2020-06-17" version="1">MLRPLLFNFIFLKMESLEVVRPELVDFKGVWMTHYFTDNWENVQNFQARPDDILIATYPKAGTTWVSYILDLLYFGQTSPERQTSMPIYLRVPFLEIAIPTIPTGKEVADELSTSPRLIKTHFPVQFVPKSFWEQNCRMIYVARNAKDNMVSYFHFDRMNSVQPVPGDWNNYFQRFLEGKNLYGSWYDHVGGWWEKTKTCSKIHYMFYEDMVEDTGREIDKLCQFLGLSAEAEEKKRVAHQAQFDSMKKNSMANYSTLPVLDFKISPFMRKGKVGDWKNHFTVAQNEEFDEDYKKKMKDPTLQFRTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVP2</accession>
    <name>A0A672ZVP2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hedgehog acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hhat</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the membrane-bound acyltransferase family. HHAT subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZVP2"/>
    <dbReference type="FunCoup" id="A0A672ZVP2">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022018.1">
        <property type="protein sequence ID" value="ENSSORP00005021375.1"/>
        <property type="gene ID" value="ENSSORG00005010450.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZVP2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016409">
        <property type="term" value="F:palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF20">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE HHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="484" mass="56329" checksum="454FBE86BC344DE7" modified="2020-06-17" version="1">MTSKPKAQVAPLPWTEILAYWVLSFGSHLYSFYELHIFSKEHEARLEREFQLEKGLVNHFKDHTDFEWSFWTEWAKKSLLWSLFWHGVISRLTSIFYPKVGTLTIFGLAAASRLLGIKGVIVLLVHLGLSFLVAQLRRPALCWACNLLLLSTLHFQTLQDIQRGWYETEEEYYLLLFSVAVCGLRFISYSQEHCWCPAQPGGAVQFCWLLSYTFYHPFFYNGPIITYKDYIQQVSFLMLRTGRILVWWCIAEYMIHMMYMHSIQSNETYLEILPPWALGGLALALVQFFYVKYLVLFGLPSMLATMDKLVPPRLPRCVSIMYSFTGMWRHFDEGLYRWLIRYIYVPLGGSRHGPLWKVFSTGLAFGFVCLWHGGHDYLQYWALMNWAGVLVENGFKSLFVSSFIHSLIEQHFSAAMKRRCIALLSAFSTAMLILSNLVFLGGIHVGRIFWKRVFIQGWSTMAPPMLAFLYCFAQIGLEWTSHPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZW96</accession>
    <name>A0A672ZW96_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Si:ch211-117k10.3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">si:ch211-117k10.3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZW96"/>
    <dbReference type="Ensembl" id="ENSSORT00005021275.1">
        <property type="protein sequence ID" value="ENSSORP00005020697.1"/>
        <property type="gene ID" value="ENSSORG00005010061.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZW96"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002639">
        <property type="entry name" value="Kruppel-Like Factor (Zinc finger protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF141">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025396770" evidence="12">
        <location>
            <begin position="17"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="312"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="342"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="64"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="155"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="407" mass="43962" checksum="413460F42B55C154" modified="2020-06-17" version="1">MLISFCLFRFFRLSLRLQFQRRPLSCVCVGSMVSLSSRTLSLENDLFRDSGLFALGLGDGASSEGGSSASCDSPGVGEPGAARSSSPAGEEEDEEEEDEEEAVHLHVFLGDAGEEDEAADQEPKLPEFPFRLPSPFSPTLEDIEEFLREKMDVVREGPVSPKEEASPLPCTSMDSPPSSAALPASSETCGDPGSSSSHCTSSPDTPKKEHNSPRPPDPSPSAPVNPSPSTVTPPMLLGAPLVLQLQPLPLAPPQTPAAPAPADQKYVKIAPLPITMRTLEITGVAGVGAPANGVLKAVAPRVSRAPTTERVHKCSHPGCGKMYTKSSHLKAHFRRHTGEKPYTCSWPECGWRFSRSDELSRHRRSHSGIKPYECSLCEKKFARSDHLSKHTKVHRSSRPSRIIRTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZX15</accession>
    <name>A0A672ZX15_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized LOC115424269</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">zgc:153292</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZX15"/>
    <dbReference type="Ensembl" id="ENSSORT00005022185.1">
        <property type="protein sequence ID" value="ENSSORP00005021539.1"/>
        <property type="gene ID" value="ENSSORG00005010542.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZX15"/>
    <dbReference type="OrthoDB" id="413122at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052224">
        <property type="entry name" value="THAP_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927">
        <property type="entry name" value="AGAP005574-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927:SF3">
        <property type="entry name" value="THAP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="196"/>
            <end position="353"/>
        </location>
    </feature>
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        <location>
            <begin position="79"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="378" mass="43030" checksum="F4C88AAEDEB30FA8" modified="2020-06-17" version="1">MGRYKCSADMCESSSESDVKFFKFPLCNSRKLKKWLANMKCKDWTPSRFSVLCINHFEEKHIDRTGKCVTLREDAVPTIFQPPDNTKKRKKASTDLGSKKVKAPRFKASQTNPAPTAPPAMTMAGVQNEAPSDTEEEPSGDPQKPERWRIIVDHGLMKISSFPHFFHGDYCVPQDIQWAPDDNSSTDNKDPENIIEVKEPWEWLGLDVRGPLPQTVNGHKYVLTVTDYFSKWVEALPMELCLPVHVAKHIVDIIAHFGFPFRILSRLPHDIVQKINKELKDRLKVSVALVVHHQQTGSADLITQQLIDRMVNSLVEEHAADWDVYLPAKVFSLCFKEHSSTKERPFSLLCCTGVEPVKSPRGLNFTYSKIRDSAFVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXQ5</accession>
    <name>A0A672ZXQ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">endothelin-converting enzyme 1</fullName>
            <ecNumber evidence="5">3.4.24.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ece2b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.</text>
            <dbReference type="EC" id="3.4.24.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Secretory vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.24.71" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZXQ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005022340.1">
        <property type="protein sequence ID" value="ENSSORP00005021693.1"/>
        <property type="gene ID" value="ENSSORG00005008845.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022510.1">
        <property type="protein sequence ID" value="ENSSORP00005021861.1"/>
        <property type="gene ID" value="ENSSORG00005008845.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6475849at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000076">
        <property type="entry name" value="membrane metallo-endopeptidase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF127">
        <property type="entry name" value="EEF1AKMT4-ECE2 READTHROUGH TRANSCRIPT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="20">
        <location>
            <begin position="114"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="19">
        <location>
            <begin position="559"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="763" mass="85869" checksum="915B2B11DD79F84F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY07</accession>
    <name>A0A672ZY07_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein tyrosine phosphatase non-receptor type 20</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZY07"/>
    <dbReference type="Ensembl" id="ENSSORT00005022005.1">
        <property type="protein sequence ID" value="ENSSORP00005021362.1"/>
        <property type="gene ID" value="ENSSORG00005010443.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZY07"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052074">
        <property type="entry name" value="NonRcpt_TyrProt_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46900:SF4">
        <property type="entry name" value="FERM AND PDZ DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46900">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="21"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="181"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="285" mass="33658" checksum="88B58BFD521E55CB" modified="2020-06-17" version="1">RWFVEPQSRPRSCLLDFWQNNKVAVFVVYDRKLTDLSRICKHKENDQTRVPIGENQDYINASYIRMQVGAEEFFYISCQGPLPSTVLAFWQMIWENKSDVIAMMTLEVERGRIKCHKYWPERLGVPLDAGKYQLHLENQQYMEYFHIKVIRMVETETGETHFVRHLKFTHWPDHGVPHCSEQLVRFIRYLRALHHKGPVTVHCSAGIGRTGVLICTDVILSLIENDLPINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQGILQLHGNQWQLDSPRDHKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY21</accession>
    <name>A0A672ZY21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">rffl</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_030008259.1">
        <property type="nucleotide sequence ID" value="XM_030152399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZY21"/>
    <dbReference type="FunCoup" id="A0A672ZY21">
        <property type="interactions" value="496"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022816.1">
        <property type="protein sequence ID" value="ENSSORP00005022160.1"/>
        <property type="gene ID" value="ENSSORG00005010813.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431753"/>
    <dbReference type="CTD" id="117584"/>
    <dbReference type="InParanoid" id="A0A672ZY21"/>
    <dbReference type="OrthoDB" id="6339724at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000110">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049320">
        <property type="entry name" value="CARP1_2_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055111">
        <property type="entry name" value="RNF34_RFFL_HeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057299">
        <property type="entry name" value="RNF34_RFFL_SAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RIFIFYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21272">
        <property type="entry name" value="FYVE_CARP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22968">
        <property type="entry name" value="RNF34L-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23632">
        <property type="entry name" value="SAP_RNF34_RFFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="291"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="135"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="338" mass="37884" checksum="4E7359275A3F12E0" modified="2020-06-17" version="1">MWASCCTWCMDSADVDESNSADQHHRSYTNSAFNNQPSPTAPEHICKACGGRFDTPAKKHVCVDCKKNYCSRCSAQLEPRPRLCHTCQRFYGNLLERAELMKLKVKELRDYLHLHEVSTHLCREKEELVDLVLSQQSSPSGGSAPYTPTSEPPDITPEPEPDPTPTPVPDPPTVQPEAPTPPTEPEPVEPEPPEPDPQDEDQTSDPEEVPIPGRRASLSDLSCLEDIEDLSVRQLKEILARNFVNYKGCCEKWELMERVRRLYQDQQNLAASTVNASEAGGNSAGLEENLCRICMDCPIDCVLLECGHMITCTKCGKRMNECPICRQYVVRAVHVFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZD7</accession>
    <name>A0A672ZZD7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZZD7"/>
    <dbReference type="Ensembl" id="ENSSORT00005023086.1">
        <property type="protein sequence ID" value="ENSSORP00005022426.1"/>
        <property type="gene ID" value="ENSSORG00005010916.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZD7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061628">
        <property type="term" value="F:histone H3K27me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062072">
        <property type="term" value="F:histone H3K9me2/3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20149">
        <property type="entry name" value="PWWP_HDGFL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000017">
        <property type="entry name" value="hepatoma-derived growth factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550:SF18">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="1">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="106" mass="12040" checksum="4EACE3797A751AA3" modified="2020-06-17" version="1">MPHNFQPGDLVFAKMKGYPHWPARIEDVADGAVKPPPNKIPIFFFGTHETAFLAPKDLFAYEKYRERYGKPNKRKGFNEGLWEIQNNPHASYSAPPVVSNAAKILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0J4</accession>
    <name>A0A673A0J4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">XRN2-binding (XTBD) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cdkn2aipnl</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CARF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030009139.1">
        <property type="nucleotide sequence ID" value="XM_030153279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A0J4"/>
    <dbReference type="Ensembl" id="ENSSORT00005023420.1">
        <property type="protein sequence ID" value="ENSSORP00005022754.1"/>
        <property type="gene ID" value="ENSSORG00005011048.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432438"/>
    <dbReference type="CTD" id="91368"/>
    <dbReference type="InParanoid" id="A0A673A0J4"/>
    <dbReference type="OrthoDB" id="2359216at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021859">
        <property type="entry name" value="XTBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48430">
        <property type="entry name" value="PARTNER OF XRN-2 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48430:SF1">
        <property type="entry name" value="PARTNER OF XRN-2 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11952">
        <property type="entry name" value="XTBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51827">
        <property type="entry name" value="XTBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XRN2-binding (XTBD)" evidence="2">
        <location>
            <begin position="20"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="108" mass="12773" checksum="40CF67E11A0A19BC" modified="2020-06-17" version="1">MSALDVKDFIQQNRALAEQIETFRGYWESDKHWEARREFILRNINDFEFPQLDQLLSMSMVWANNVFLGCRYSAELLDKVKEMAEGIVVEDAPVFKTRDEIVKKQQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1D3</accession>
    <name>A0A673A1D3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Prostamide/prostaglandin F synthase</fullName>
            <ecNumber evidence="13">1.11.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxiredoxin-like 2B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">prxl2b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of prostaglandin-ethanolamide H(2) (prostamide H(2)) to prostamide F(2alpha) with NADPH as proton donor. Also able to reduce prostaglandin H(2) to prostaglandin F(2alpha).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>prostaglandin H2 + [thioredoxin]-dithiol = prostaglandin F2alpha + [thioredoxin]-disulfide</text>
            <dbReference type="Rhea" id="RHEA:28214"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="EC" id="1.11.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>prostamide F2alpha + [thioredoxin]-disulfide = prostamide H2 + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:26373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:53081"/>
            <dbReference type="ChEBI" id="CHEBI:53082"/>
            <dbReference type="EC" id="1.11.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peroxiredoxin-like PRXL2 family. Prostamide/prostaglandin F synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.20" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A673A1D3"/>
    <dbReference type="FunCoup" id="A0A673A1D3">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023207.1">
        <property type="protein sequence ID" value="ENSSORP00005022542.1"/>
        <property type="gene ID" value="ENSSORG00005010968.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A1D3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047017">
        <property type="term" value="F:prostaglandin F synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02970">
        <property type="entry name" value="PRX_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000243">
        <property type="entry name" value="Prostamide/prostaglandin F synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032801">
        <property type="entry name" value="PXL2A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630:SF28">
        <property type="entry name" value="PROSTAMIDE_PROSTAGLANDIN F SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13911">
        <property type="entry name" value="AhpC-TSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="230" mass="25081" checksum="454132CD47B79941" modified="2020-06-17" version="1">MANINLAVVGNNLVKSAETGETVELKSLWQDQPVVLFFLRRFGCQVCRWMASEISKLEPDLTASGVSLVGIGPEELGLKEFKEGGFFKGSLYVDEKKKSYKDLGFKRYSALSVVPAALGKKVRDVITKANAVGIQGNFSGDLLQSGGMPHCGGESILLHFIQDSPGDHVPLEDISKALGISAQVKAGQMPVVRSFRSVTYFTHVFVCCHCSIFTFNCYLLIISQPSDVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3K3</accession>
    <name>A0A673A3K3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A3K3"/>
    <dbReference type="Ensembl" id="ENSSORT00005023906.1">
        <property type="protein sequence ID" value="ENSSORP00005023232.1"/>
        <property type="gene ID" value="ENSSORG00005011250.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A3K3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000039">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000041">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="125" mass="14127" checksum="7458B81A71335F4C" modified="2020-06-17" version="1">MRGGKAFKAKYKLINHIREKPFQCEFCERRFANSSDRKKHSQVHTASKPYDCKALGCTKSYTHPSSLRKHMKVHVKSSPTSELQDTLIFTCSHAKPSSEMVGTCGPRLKAQSKPKGPKGITSYIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3Q8</accession>
    <name>A0A673A3Q8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A3Q8"/>
    <dbReference type="Ensembl" id="ENSSORT00005024574.1">
        <property type="protein sequence ID" value="ENSSORP00005023874.1"/>
        <property type="gene ID" value="ENSSORG00005011533.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A3Q8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000002">
        <property type="entry name" value="Gap junction protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF49">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="160"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="271" mass="31522" checksum="7D9FA1B04B03B1F3" modified="2020-06-17" version="1">MGDWNLLGKLLESAQEHSTVVGKVWLTVLFIFRILVLGTAAEKVWGDEQSGFTCDTKQPGCQNVCYDKTFPISHIRFWVMQIIFVSTPTLIYLGHILHLVRMEEKEKEKEKELALHNEKQQQVVCSKAKKGPIKDNQGHVRLKGALLRTYVFNIIFKTLFEVAFIVAQYFLYGFELKPMYTCDRWPCPNMVNCYISRPTEKTIFILFMLAVACISLLLNLVEMYHLGFTKCHQGLRYRRSRNATEASKAMSENIQCHRISHIKKTRMNQEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A437</accession>
    <name>A0A673A437_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115417608</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005024733.1">
        <property type="protein sequence ID" value="ENSSORP00005024024.1"/>
        <property type="gene ID" value="ENSSORG00005011595.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A437"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.80.10">
        <property type="entry name" value="Lipovitellin-phosvitin complex, chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.50.20">
        <property type="entry name" value="Lipovitellin. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.90.10">
        <property type="entry name" value="Vitellinogen, beta-sheet shell domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015258">
        <property type="entry name" value="Vitellinogen_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037088">
        <property type="entry name" value="Vitellinogen_b-sht_shell_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015817">
        <property type="entry name" value="Vitellinogen_open_b-sht_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050733">
        <property type="entry name" value="Vitellogenin/Apolipophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345:SF9">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09175">
        <property type="entry name" value="Vit_b-sht_shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01170">
        <property type="entry name" value="DUF1944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Vitellogenin" evidence="8">
        <location>
            <begin position="12"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="9">
        <location>
            <begin position="1291"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="980"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1028"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="146"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1536" mass="172451" checksum="18ACD41584552F58" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A937</accession>
    <name>A0A673A937_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Hyaluronan and proteoglycan link protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115435084</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the HAPLN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A937"/>
    <dbReference type="Ensembl" id="ENSSORT00005025838.1">
        <property type="protein sequence ID" value="ENSSORP00005025083.1"/>
        <property type="gene ID" value="ENSSORG00005012073.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025852.1">
        <property type="protein sequence ID" value="ENSSORP00005025097.1"/>
        <property type="gene ID" value="ENSSORG00005012082.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03519">
        <property type="entry name" value="Link_domain_HAPLN_module_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000001">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF8">
        <property type="entry name" value="HYALURONAN AND PROTEOGLYCAN LINK PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-0373">Hyaluronic acid</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="61"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="12">
        <location>
            <begin position="171"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="12">
        <location>
            <begin position="268"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="361" mass="40602" checksum="C208F9D582E3B43C" modified="2020-06-17" version="1">MKDKQGILQFFFHLVCSMYACSTFHVLFDGMSIWLQENMGHPTFNLCLLAEPTELKYLIEPPVYAEVSGRRGENVTLPCMLRTKPHHYKVKWTKLEPERTGPENIVLISNSRAFKTYGLLGPRASLRKAHAMDASLQLSHLELGDGGGYRCQLVNGIEDESVDVTLTIEGVVFPYQSKNGRYRFTFHEAKQACAEQDGTLASYNQLYRAWTEGLDWCNAGWLRDGTVHYPIIQLRPACGGALLPGIRSYGPKDKNHDRFDAFCFTSLASGSIFFVSGSFSFVQAQRACEQQGADLALVGQLYSAWRFHSYDQCDGGWLKDGSVRFPISHPRERCGGVPEAGVRSFGFPDQKTHVYGAYCYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAR1</accession>
    <name>A0A673AAR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 2 subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 2 subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">eif2s2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029995129.1">
        <property type="nucleotide sequence ID" value="XM_030139269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AAR1"/>
    <dbReference type="Ensembl" id="ENSSORT00005027452.1">
        <property type="protein sequence ID" value="ENSSORP00005026670.1"/>
        <property type="gene ID" value="ENSSORG00005012777.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422757"/>
    <dbReference type="CTD" id="8894"/>
    <dbReference type="InParanoid" id="A0A673AAR1"/>
    <dbReference type="OrthoDB" id="10255414at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="7">
        <location>
            <begin position="198"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="332" mass="37781" checksum="E80A529DD4254C63" modified="2020-06-17" version="1">MSGDEMIFDPNMTKKKKKKKKPFMLDEDGGEGMGGEEAKEVEAKEVEPEAGDERDLDLDEDEGRKKEPSDDLNDLNFFNQKKKKKKPKKVFDNDVEEGLKELKIEGEQPEALEEDNLDLMLPAKKKKSKKVDFDEGDALEKDDALEDDEGKNNDGISFSSSTGPAWAGSERDYTYDELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPETILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAS4</accession>
    <name>A0A673AAS4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal L1 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115439703</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Regulates cellular senescence through inhibition of PTEN translation. Acts as a pro-apoptotic regulator in response to DNA damage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the universal ribosomal protein uL1 family. Highly divergent.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AAS4"/>
    <dbReference type="FunCoup" id="A0A673AAS4">
        <property type="interactions" value="1039"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027052.1">
        <property type="protein sequence ID" value="ENSSORP00005026279.1"/>
        <property type="gene ID" value="ENSSORG00005012608.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AAS4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000004">
        <property type="entry name" value="Ribosomal L1 domain-containing 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="257"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="373" mass="42383" checksum="2DA6B5D79BC1B361" modified="2020-06-17" version="1">MAENTELAIDRAQVKKAVQALQAFLKTKSSSDILLLNETQHITLLFTLWKVPKQPQTIRIPLPHGQRSDTEEVCLFTKDEPNMTSEQTQRFYKKLLEERGVKNVTEIIPFKVLKTEYKPYEAKRRLLANYDLFLSDDRIRRLLPSHLGRHFYERKKEPLSVNLQSKHLARDIEKVIQGTSMKISNRGCCCMARVAHSDMTADEVTDNIEAAIQTFVAKLRAKGPMIKLIHLKSQTSVALPIYTSDMSTLTVLEEALKSSKTPKDKKVAAKKRGKKNKSQDIKQKDTITEKKQVKGKKEAEKREKEDDDDEIPQLVPIETPSKKPKLEKTPKKKQLEKAPKPAVVKKGTKGPNKLTKKAGKTGKEGKTKVPKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADT5</accession>
    <name>A0A673ADT5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115439076</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ADT5"/>
    <dbReference type="FunCoup" id="A0A673ADT5">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028521.1">
        <property type="protein sequence ID" value="ENSSORP00005027725.1"/>
        <property type="gene ID" value="ENSSORG00005013257.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ADT5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051016">
        <property type="entry name" value="Diverse_Substrate_AcTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545:SF66">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="94"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="163" mass="18331" checksum="ADD19BC245EC02C8" modified="2020-06-17" version="1">MDFSIRAANVEDCKDIARMITELAEYEKVTDQVKITQRDLEQDGFSKNPFFHGIIAEVPEQHKSKEGDGKNTDSNRNKTTVASDKKWLYLCFLGKGIGKALMSKVAQVIGSGRGCHQLNFTVLDWNKSSVDFYLHQGCTDVTTDMGYHYMSCKGEAMEHLAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJ23</accession>
    <name>A0A673AJ23_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115419638</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AJ23"/>
    <dbReference type="Ensembl" id="ENSSORT00005029111.1">
        <property type="protein sequence ID" value="ENSSORP00005028302.1"/>
        <property type="gene ID" value="ENSSORG00005013447.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJ23"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000052">
        <property type="entry name" value="semaphorin-3F isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF27">
        <property type="entry name" value="SEMAPHORIN-3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025530639" evidence="12">
        <location>
            <begin position="20"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="14">
        <location>
            <begin position="23"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="599"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="754"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="770"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="782" mass="88380" checksum="E6452632E787D9D3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJA3</accession>
    <name>A0A673AJA3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Activation-induced cytidine deaminase</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AJA3"/>
    <dbReference type="Ensembl" id="ENSSORT00005030264.1">
        <property type="protein sequence ID" value="ENSSORP00005029431.1"/>
        <property type="gene ID" value="ENSSORG00005014050.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013158">
        <property type="entry name" value="AID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050610">
        <property type="entry name" value="APOBEC_Cyt_Deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13857">
        <property type="entry name" value="MRNA EDITING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13857:SF10">
        <property type="entry name" value="SINGLE-STRANDED DNA CYTOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08210">
        <property type="entry name" value="APOBEC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="6">
        <location>
            <begin position="39"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="203" mass="23958" checksum="A66556187939E2F5" modified="2020-06-17" version="1">MSTYVSSKHKYHIVSLCFSVLLPRKKFIYHYKNMRWARGRHETYLCFVVKRRVGPDSLSFDFGHLRNRNGCHVEVLFLRYIGALCPGLWGCGGTGERRLSYSITWFCSWSPCANCSFRLAQFLSQTPNLRLRIFVSRLYFCDFEGTDSEGLRMLKKAGVQITVMTYKDYFYCWQTFVARKQSHFKAWEGLHQNSVRLARKLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJC0</accession>
    <name>A0A673AJC0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Golgin A7 family, member Bb</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ERF4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AJC0"/>
    <dbReference type="Ensembl" id="ENSSORT00005029211.1">
        <property type="protein sequence ID" value="ENSSORP00005028402.1"/>
        <property type="gene ID" value="ENSSORG00005013587.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJC0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002178">
        <property type="term" value="C:palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF2">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="4">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="146" mass="16807" checksum="B91F6673EE17C016" modified="2020-06-17" version="1">VFHNLQELRHSASLVTKVFIQRDYSEGTVCRFQTKFPSELDNRIERTLLEQTVKTLNSYYVEAEKIGGQSYLEGCLACATAYIIFLCMETRYEKVLKKISGYIQEQNEKIYAPRGLLLTDPIERGMRVVSFTYQDRTAKGKSVSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJT3</accession>
    <name>A0A673AJT3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenosine receptor A2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">adora2aa</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12 14">Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct. May interact with DRD4. Interacts with NECAB2. Interacts (via cytoplasmic C-terminal domain) with GAS2L2; interaction enhances receptor-mediated adenylyl cyclase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AJT3"/>
    <dbReference type="FunCoup" id="A0A673AJT3">
        <property type="interactions" value="601"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030459.1">
        <property type="protein sequence ID" value="ENSSORP00005029626.1"/>
        <property type="gene ID" value="ENSSORG00005014143.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJT3"/>
    <dbReference type="OrthoDB" id="9445642at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001609">
        <property type="term" value="F:G protein-coupled adenosine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000061">
        <property type="entry name" value="Adenosine receptor A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001513">
        <property type="entry name" value="Adeno_A2A_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001634">
        <property type="entry name" value="Adenosn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF47">
        <property type="entry name" value="ADENOSINE RECEPTOR A2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00553">
        <property type="entry name" value="ADENOSINA2AR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00424">
        <property type="entry name" value="ADENOSINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="25"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU201114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="446" mass="49369" checksum="33047B3F77A806E4" modified="2020-06-17" version="1">MPEDPIASVFYIILELLIALFSVLGNVLVCWAVCLNSNLQSITNFFVVSLAVADIAVGVLAIPFAIVISTGFCSNFYGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIKLPLRYNSLVTGQRARGIIAICWVLSIIIGLTPMMGWHKEPEMDNGTCPPGLMKCLFEGVVDMEYMVYFNFFACVLIPLLLMLAIYLCIFMAARHQLKLMEVKAVHGEKSRSTLQKEVQAAKSLAIIVGLFAVCWLPLHIINCFTLFCPKCERCPLWVINVAIILSHANSVVNPFIYAYRIREFRQTFHKIIHRHILGKQDLMESRGSTRTSTHNSISDSIRLKANGLSFDVFSEHSSSSSCESSCRCPAQASPVGGGLMTVSHPPLSIVIAHCPKIGPLQGLRQTQIAVGQHLQYTDCVGAEAVEEKDKHSLQSTHNALLLNKQNRKSCCRELVKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AL60</accession>
    <name>A0A673AL60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AL60"/>
    <dbReference type="Ensembl" id="ENSSORT00005031279.1">
        <property type="protein sequence ID" value="ENSSORP00005030425.1"/>
        <property type="gene ID" value="ENSSORG00005014510.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AL60"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF22">
        <property type="entry name" value="PEPSIN A-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="109"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="216" mass="23661" checksum="EB14DEA896E1A4ED" modified="2020-06-17" version="1">MTGFLGYDTVTVGGLAVKNQVFGLSETEAPFMQYIHPDGILGLAYPLSASRATPVFDNMMKQSLVSQDLFSVYLSSHFSGSISWIPLSHELYWQISVDSVTINGQVVACNGGCQMIVDTGTSLIAGPQNDISNINRYVGANGNQYGDYLVNCNSVAQMPDLTFQTMDRIGLCGLVCLSDCCLCLQSHLWILGDVFIRHYYSIFNRAQNMVGLARSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALB9</accession>
    <name>A0A673ALB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spermatogenesis-associated protein 20-like TRX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spata20</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ALB9"/>
    <dbReference type="FunCoup" id="A0A673ALB9">
        <property type="interactions" value="801"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031334.1">
        <property type="protein sequence ID" value="ENSSORP00005030480.1"/>
        <property type="gene ID" value="ENSSORG00005014525.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALB9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02955">
        <property type="entry name" value="SSP411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 20-like TRX" evidence="1">
        <location>
            <begin position="45"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="761" mass="84612" checksum="A8319467021CBFCA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANF6</accession>
    <name>A0A673ANF6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol-3-phosphate phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.21</ecNumber>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartate-based ubiquitous Mg(2+)-dependent phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphoglycolate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pgp</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear. In vitro, also has a phosphatase activity toward ADP, ATP, GDP and GTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>sn-glycerol 1-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:46084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sn-glycerol 3-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:66372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="19">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.21" evidence="8"/>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_029977487.1">
        <property type="nucleotide sequence ID" value="XM_030121627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ANF6"/>
    <dbReference type="FunCoup" id="A0A673ANF6">
        <property type="interactions" value="789"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030784.1">
        <property type="protein sequence ID" value="ENSSORP00005029942.1"/>
        <property type="gene ID" value="ENSSORG00005014306.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410152"/>
    <dbReference type="CTD" id="283871"/>
    <dbReference type="InParanoid" id="A0A673ANF6"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043136">
        <property type="term" value="F:sn-glycerol 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006114">
        <property type="term" value="P:glycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000123">
        <property type="entry name" value="glycerol-3-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF92">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01139">
        <property type="entry name" value="C2.A:_Pyridoxal_Phosphate_Phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="305" mass="32937" checksum="FAE7A60399CF3F79" modified="2020-06-17" version="1">MSGSKCTRLSGALVKQLLDSVDSVLFDCDGVIWRGDQAIPGAPQVVNLLKEKGKKVFFVTNNSTKTRKMYADKMSTLGFNVTEEEVFGTAYCSAMYLKTVCKLDGKVYLIGSNALKQELEAVGIQQTGVGPDHVAGKQADWANVPLDPEVKAVLVGFDEHFSYMKLNRALQYLIQGRLFVGTNSDTRLPLEGGKAVPGTGCLLQAVETAAQRKAETVGKPNHYMFDCVASQFSVDSERCLMVGDRLDTDILFGSNCGLKTLLTLTGVSTVADAEAHQKSGCADRQGMVPDYYVESIADLLPALQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQK2</accession>
    <name>A0A673AQK2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arf-GAP domain and FG repeat-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">agfg1b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AQK2"/>
    <dbReference type="FunCoup" id="A0A673AQK2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032835.1">
        <property type="protein sequence ID" value="ENSSORP00005031950.1"/>
        <property type="gene ID" value="ENSSORG00005015176.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQK2"/>
    <dbReference type="OrthoDB" id="6036at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007289">
        <property type="term" value="P:spermatid nucleus differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08857">
        <property type="entry name" value="ArfGap_AGFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000005">
        <property type="entry name" value="Arf-GAP domain and FG repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052248">
        <property type="entry name" value="Arf-GAP_FG-repeat_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134:SF5">
        <property type="entry name" value="ARFGAP WITH FG REPEATS 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134">
        <property type="entry name" value="DRONGO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="11"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="145"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="245"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="469" mass="49576" checksum="329F6F09BACE93F0" modified="2020-06-17" version="1">MATSAKRKQEETHLKMLREMTSLPANRKCFDCDQRGPTYVNMTVGSFVCTTCSGILRGLNPPHRVKSISMTTFTQQEIEFLQKHSNEVCKHIWLGLYDDRTSVVPDFREPQKVKEFLQEKYEKKRWYVPPDQARAVASVQASVSGSSASSTGSTPEVQPLKTLQLNKTPLRQSPGLGRSQAHTAAQEKKFDLLSDLGGDIFAAPPSQTASSTNFANFAHFQSQSAPQGHSNTNFANFEAFGNTAIPSHLSTSPPSKSFPSGGSVPIPSMSSAVPSQSQTGSCTEDRYAALAELDNELSSSVSSGSNVPGNIFGPVLGSSPAQNPPVLPSMQPGFGAVPSTNPFVAAAVAPEMATNPFQTNGRAPSAASFGTGSMSMPAGFGNASSYCLPTSFSGNFQQQFPGQAPIPYSQPGGYHPQSNGPAFPVYGQNKPSMTPFGQPMAGPGMSNNPFMAGAPAGPFPSGGSTNPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARY9</accession>
    <name>A0A673ARY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ARY9"/>
    <dbReference type="Ensembl" id="ENSSORT00005032986.1">
        <property type="protein sequence ID" value="ENSSORP00005032099.1"/>
        <property type="gene ID" value="ENSSORG00005015254.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARY9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SH2" evidence="1">
        <location>
            <begin position="10"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="89" mass="10278" checksum="E845E2058004155E" modified="2020-06-17" version="1">RLSHPHLLIGSEATEMLLERFGRDGSFLLRDSDTVRGAYCLCVRWVKMDTFCRKFTCKELNSNQPTDLKCLITLKPLIESIRVNDSDKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASD8</accession>
    <name>A0A673ASD8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatidylinositol transfer protein cytoplasmic 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pitpnc1b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ASD8"/>
    <dbReference type="Ensembl" id="ENSSORT00005033371.1">
        <property type="protein sequence ID" value="ENSSORP00005032481.1"/>
        <property type="gene ID" value="ENSSORG00005015416.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASD8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000028">
        <property type="entry name" value="Phosphatidylinositol transfer protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF83">
        <property type="entry name" value="CYTOPLASMIC PHOSPHATIDYLINOSITOL TRANSFER PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="3">
        <location>
            <begin position="43"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="339" mass="38828" checksum="205999AF2C17A8BC" modified="2020-06-17" version="1">MSFEIVLLIYLSLTFSHGSFFLFLSFPFKPTPKLHPECLLGLQYKIGQLYMISKYSCEESGGGEGVEVVRNESDVHPQHGAGQLTEKRIYLSSKLPSWAKSFVPRFFYVTEKAWNFYPYTITGAYVCASFLPKFSIRIETRFENNNGDNDNVFGDTPTPAGNVSFLDILSDPIPEKHYKESEDLSRWQSSKTGRGPLKDGWRDDHRPIMCSYKRVCCSFEVYGFQTRTEEFIHRNIRDILLVGHRQAVAWIDEWHGLNMEEVREYERTMQEKTNSKKNLLVYPSICLPLSDPSAAFRPRSISESDERSLKRMGVVNAAMSDPPASSIAKSPMRLNSTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASP7</accession>
    <name>A0A673ASP7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rho-related GTP-binding protein RhoA-C-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115422748</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029995121.1">
        <property type="nucleotide sequence ID" value="XM_030139261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ASP7"/>
    <dbReference type="Ensembl" id="ENSSORT00005033241.1">
        <property type="protein sequence ID" value="ENSSORP00005032354.1"/>
        <property type="gene ID" value="ENSSORG00005015342.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422748"/>
    <dbReference type="InParanoid" id="A0A673ASP7"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000095">
        <property type="entry name" value="Rho-related GTP-binding protein RhoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="193" mass="21851" checksum="ED9EBB133ACAA442" modified="2020-06-17" version="1">MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDDHTRRELAKMKQEPVKYEDGKEMANRISAYGYQECSAKTKDGVREVFEMATRAALQAKKRGKKSTCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUB9</accession>
    <name>A0A673AUB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Neurabin-I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ppp1r9a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AUB9"/>
    <dbReference type="Ensembl" id="ENSSORT00005032827.1">
        <property type="protein sequence ID" value="ENSSORP00005031942.1"/>
        <property type="gene ID" value="ENSSORG00005015183.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUB9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09512">
        <property type="entry name" value="SAM_Neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000008">
        <property type="entry name" value="Neurabin-1 isoform 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040645">
        <property type="entry name" value="Neurabin-1/2_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043446">
        <property type="entry name" value="Neurabin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154">
        <property type="entry name" value="NEURABIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154:SF22">
        <property type="entry name" value="NEURABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17817">
        <property type="entry name" value="PDZ_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="501"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="18">
        <location>
            <begin position="893"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="961"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="597"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="675"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="993" mass="111268" checksum="2AD76DC43F7F0927" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVA0</accession>
    <name>A0A673AVA0_9TELE</name>
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        <recommendedName>
            <fullName evidence="12">Nedd4 family interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115432747</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AVA0"/>
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    </dbReference>
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        <property type="gene ID" value="ENSSORG00005015734.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="term" value="F:WW domain binding"/>
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    </dbReference>
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        <property type="term" value="P:metal ion transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of protein ubiquitination"/>
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    </dbReference>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
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    </dbReference>
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        <property type="term" value="P:vacuolar transport"/>
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    </dbReference>
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        <property type="entry name" value="NEDD4/Bsd2"/>
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    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF3">
        <property type="entry name" value="NEDD4 FAMILY-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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            <end position="159"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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        <location>
            <begin position="1"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24382" checksum="444835E78AE34DB0" modified="2020-06-17" version="1">MPTPSKHGLPFFPQLPNEEDPEESPQAVADAPPPYSSIAAVNAAFFEYKEDGAFPKPPSYNVATTLPSYDEAERTKAETTVPLVTGRDDEFVTRDDFEDADQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLTTSAAGRYGAISGFGLSLIKWILIVRFSTYFPGYFDGQYWLWWVFLVLGFLLFLRGFINYARIRKMADTLSTLPRTRVLFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVG9</accession>
    <name>A0A673AVG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acbd7</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AVG9"/>
    <dbReference type="FunCoup" id="A0A673AVG9">
        <property type="interactions" value="1071"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033215.1">
        <property type="protein sequence ID" value="ENSSORP00005032328.1"/>
        <property type="gene ID" value="ENSSORG00005015334.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AVG9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00435">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF51">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="16"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="101" mass="11239" checksum="4F653A646B7B0A78" modified="2020-06-17" version="1">ISNLDHNSDVAGCFLLQAEFEKVAEDVKKVKTRPSDQELLDLYGLYKQAVVGDVNINRPGMADLKGKAKWDAWNSRKGMSKEDAMSAYITLAKEIIGKYGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVP6</accession>
    <name>A0A673AVP6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription cofactor HES-6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">her13</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AVP6"/>
    <dbReference type="Ensembl" id="ENSSORT00005034648.1">
        <property type="protein sequence ID" value="ENSSORP00005033735.1"/>
        <property type="gene ID" value="ENSSORG00005015970.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AVP6"/>
    <dbReference type="OrthoDB" id="6085656at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11460">
        <property type="entry name" value="bHLH-O_HES6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000112">
        <property type="entry name" value="Hes family bHLH transcription factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="27"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="8">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="171"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="239" mass="26484" checksum="8569B9A2E8CEEF48" modified="2020-06-17" version="1">MAPSARPSKNGLGMDEDDSFYGVQKTDRKIRKPLVEKKRRARINESLQELRTLLADTDFHSKMENAEVLEMTVKKVEDILKNRTQESDALNREASERFAAGYIQCMHEVHMFVSSCPGIDSTVAAELLNHLLECMPLNEDHLQDVLMDLIADTSGNSSTWHGGGETLCTSLASPGGRSLSSGSSSVLSPAPSTISSEDLCSDLDETDSEHNQSSTEGLENREALSMPTMTYPWSMWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AW57</accession>
    <name>A0A673AW57_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GDNF family receptor alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">gfra1b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GDNFR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AW57"/>
    <dbReference type="Ensembl" id="ENSSORT00005034463.1">
        <property type="protein sequence ID" value="ENSSORP00005033554.1"/>
        <property type="gene ID" value="ENSSORG00005015887.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AW57"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.110:FF:000001">
        <property type="entry name" value="GDNF family receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.110">
        <property type="entry name" value="GDNF binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017372">
        <property type="entry name" value="Glial_neurotroph_fac_rcpt_a1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF3">
        <property type="entry name" value="GDNF FAMILY RECEPTOR ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038071">
        <property type="entry name" value="GDNF_family_receptor_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01316">
        <property type="entry name" value="GDNFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="13">
        <location>
            <begin position="31"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="13">
        <location>
            <begin position="152"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="13">
        <location>
            <begin position="245"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="12">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="38"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="152"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="159"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="186"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="215"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="245"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="252"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="279"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038071-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="455" mass="51064" checksum="47217DFBD52ADDFA" modified="2020-06-17" version="1">MINTNFGSVLATFWLDYGDSGSPSRSVHLDCVKASEQCLKEYSCSTKYRTMRQCVAGRESNFSAVTGPEAQGECRSAIEAMKQSPLYRCRCRRGMKKEKNCLRIFWSIFESLQGNDLLEYSPYEPVNSRLSDIFRLAPIIAVEPASTKENNCLNAAKACNLNDTCKKYRSAYINPCTSRVSASEVCNKRKCHKALRQFFDKVPTKYSYGMLFCSCPAGDQTACAERRRQTIVPVCSYEDKDKPNCLSLQNTCKTNYICRSRLADFISNCQPEVRSISGCLRENYADCLLSYSGLIGTVMTPNYVRSLGISLSPWCDCNSSGNNKPDCDKFADFFTDNRCLRNAIQAFGNGTDVGVWQPQPPIQPTVVEPGITRKGKERTSNSVDALTDMTGMDLDADSLYQICGMMQVQIRTFQIHCELKSSYFTKPTFISRRFIFCQKLISWTVNTSAAVNVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AXG7</accession>
    <name>A0A673AXG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibroblast growth factor</fullName>
            <shortName evidence="2">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AXG7"/>
    <dbReference type="Ensembl" id="ENSSORT00005033961.1">
        <property type="protein sequence ID" value="ENSSORP00005033057.1"/>
        <property type="gene ID" value="ENSSORG00005015682.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AXG7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="133" mass="14509" checksum="4898C5C94066E4F6" modified="2020-06-17" version="1">QKRNVSLKSVQPGQVVIKGQSTSLFLCVDGGGTLSGQSQYTEADCTFTELLLADGYTRFLSAHHGFPVSLAPKHSMDRHAIPFTRFLPLRNTLRLEGVSEQPASNQKYFNVDSDDPLGMGLNAVVSPHFSSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZR6</accession>
    <name>A0A673AZR6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AZR6"/>
    <dbReference type="Ensembl" id="ENSSORT00005036090.1">
        <property type="protein sequence ID" value="ENSSORP00005035155.1"/>
        <property type="gene ID" value="ENSSORG00005016586.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZR6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="126" mass="13972" checksum="4A845E26E1C7E010" modified="2020-06-17" version="1">PPPSIHGHSPHSTGPLLLSTDELTVPALYPSSPDVWASYPLYPAELSPALPPAFTYPSSLHAQVTCRKPRPFLFSLPPSPVTPQITLSSIPQTHLRPHPPTHRRHLNIYNQNRVKTNLQLEFSGFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B214</accession>
    <name>A0A673B214_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine/threonine-protein kinase SBK2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">si:ch211-171h4.3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B214"/>
    <dbReference type="FunCoup" id="A0A673B214">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035610.1">
        <property type="protein sequence ID" value="ENSSORP00005034687.1"/>
        <property type="gene ID" value="ENSSORG00005016355.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B214"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001434">
        <property type="entry name" value="Si:ch211-171h4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF34">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="40"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="370" mass="41698" checksum="AEE0299D8BB79BB5" modified="2020-06-17" version="1">MFSRGSSEATNTMTAATKLLDELCHVTAQSLCSMDTDEHFHVLKLLGEGSYGKVMLAMHRKRGTPMALKFFPRESTSLLSFLREYNLSLSFCTHPSLTTALGIAYSTPSHYIFAQQPSLFGDLYDVITPEVGMEEDCCQRVVSQLCGALSHLHRLGFVHRDLKPENIFLCDAACRWVKLGDFGMVKARGTKVPEVWYSSPYCTPEAEVARGNQDSWREMKEDNGHQRTRTGTRTRTRVWVSVEPSTDSWALGILTYAMLTGSHPWAETSGDCRSYLKYRQWVDGVKSPGDGLDVWAEPQGDTRPNRQKDGPPVAPQFTCFTPLACSLFLSLLDPRPKHRGQPEDMLAFLGGQWMVEKERERLEEERKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2L6</accession>
    <name>A0A673B2L6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Regulator of G protein signaling 12b</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B2L6"/>
    <dbReference type="Ensembl" id="ENSSORT00005035835.1">
        <property type="protein sequence ID" value="ENSSORP00005034907.1"/>
        <property type="gene ID" value="ENSSORG00005016447.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2L6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08742">
        <property type="entry name" value="RGS_RGS12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003116">
        <property type="entry name" value="RBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046995">
        <property type="entry name" value="RGS10/12/14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037880">
        <property type="entry name" value="RGS12_RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BC83"/>
    <dbReference type="Ensembl" id="ENSSORT00005041117.1">
        <property type="protein sequence ID" value="ENSSORP00005040081.1"/>
        <property type="gene ID" value="ENSSORG00005018616.1"/>
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    <dbReference type="InParanoid" id="A0A673BC83"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:synaptobrevin 2-SNAP-25-syntaxin-1a complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:terminal bouton"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:soluble NSF attachment protein activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:syntaxin binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:regulation of synaptic vesicle priming"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:SNARE complex disassembly"/>
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        <property type="entry name" value="beta-soluble NSF attachment protein-like isoform X1"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
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    </dbReference>
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        <property type="entry name" value="NSF_attach"/>
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    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF23">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36565" checksum="4808D75DC9009DD2" modified="2020-06-17" version="1">MDNSGKEKEATALMAEAEKKMKSSQSFFGALFGVWYWCELCMHLTSFASLFNQSHLFFHSGGSKMEEACDMYVRAANMYKMAKNWCAAGNAFSQAARLHLQMQSKHDAATNFIDAGNAFKKADPQEAINCLNRAIEIYTDMGRFTIAAKHHITIAEIYETELVDIDKAVAHYEQAADYYKGEESTSSANKCLLKVATYAAQLEQYPKAIEIYEQVGTHAMDSTLLKYSAKDHFFKAALCHFCVDAHNAQLAVQKYEEMFPAFSDSRECKLLKKLLDAHEEQNVDAYTDSVKEFDTISRLDQWLTTMLLRIKKTIQDDESDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BDE8</accession>
    <name>A0A673BDE8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Small G protein signaling modulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sgsm2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RUTBC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BDE8"/>
    <dbReference type="Ensembl" id="ENSSORT00005041592.1">
        <property type="protein sequence ID" value="ENSSORP00005040541.1"/>
        <property type="gene ID" value="ENSSORG00005018905.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Small G protein signaling modulator 1"/>
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    </dbReference>
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        <property type="entry name" value="small G protein signaling modulator 1"/>
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    </dbReference>
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        <property type="entry name" value="Small G protein signaling modulator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.230:FF:000001">
        <property type="entry name" value="Small G protein signaling modulator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
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    </dbReference>
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        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
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    </dbReference>
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        <property type="entry name" value="Rab-GAP-TBC_dom"/>
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        <property type="entry name" value="Rab-GAP_TBC_sf"/>
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    </dbReference>
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        <property type="entry name" value="Run_dom_sf"/>
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        <property type="entry name" value="SGSM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021935">
        <property type="entry name" value="SGSM1/2_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF194">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12068">
        <property type="entry name" value="PH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="7">
        <location>
            <begin position="24"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="600"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="693"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="754"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="699"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1029" mass="116672" checksum="CEFDE645B92FBDEF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BF57</accession>
    <name>A0A673BF57_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Si:ch211-195d17.2</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BF57"/>
    <dbReference type="FunCoup" id="A0A673BF57">
        <property type="interactions" value="369"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041435.1">
        <property type="protein sequence ID" value="ENSSORP00005040395.1"/>
        <property type="gene ID" value="ENSSORG00005018863.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BF57"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000458">
        <property type="entry name" value="E2F-like (Mammalian transcription factor)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015633">
        <property type="entry name" value="E2F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081:SF19">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="THAP-type" evidence="14">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="102"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="386" mass="42150" checksum="64838F7DADAD9F46" modified="2020-06-17" version="1">MVKCVVSGCPNRRGGGSGPRSSSSRGPRSLLNRPPKRFFPFPKDPTRVKVWLAALRETDKQDSTDLHMICEDHFLPEDVSTAGVNSDAIPIMPPYLDGPLGLTGAWGAESSEEQDQWAPGGCDEDEDDGGQADAAEPPSTTPQAVAEVRPQDPVQTSENPLEADKSSGTQQKAWIQPRGHYRHEVSLAVLTRRFLELLLAAPDRTLDLRQVVTSLKTRKRRIYDITNVLDGINLIQKESANRIGWIGKCPISSLLWTNQQKFQTELDNLKLVEDTLDGLIKSCAQQLFDMTDDVENAAYPFTATRLTLTPTLALTLTLTLTLTLALTLALTLTLTLTLTLALTLTLTLALTLTLTLTLTLTLALTLTLTLALTLTLTLTLALTLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BHD1</accession>
    <name>A0A673BHD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cyclin-dependent kinase 4</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cell division protein kinase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">cdk4</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="RefSeq" id="XP_029991249.1">
        <property type="nucleotide sequence ID" value="XM_030135389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BHD1"/>
    <dbReference type="Ensembl" id="ENSSORT00005042226.1">
        <property type="protein sequence ID" value="ENSSORP00005041165.1"/>
        <property type="gene ID" value="ENSSORG00005019181.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420148"/>
    <dbReference type="CTD" id="1019"/>
    <dbReference type="InParanoid" id="A0A673BHD1"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000124">
        <property type="entry name" value="Cyclin-dependent kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000205">
        <property type="entry name" value="Cyclin-dependent kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF129">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="9"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="306" mass="34432" checksum="830341F27F182103" modified="2020-06-17" version="1">MAHATSIQYEPVAEIGGGAYGTVYKARDTESGQFVALKSVRVQTDQNGLPVSTVREVALLRRLEQFDHPNVVRLMDVCATKRSDQETKVTLVFEHVDQDLKTYLERAPAPGLSPERIKDLMKQLLCGLAFLHTNRVMHRDLKPENILVTSQGQVKLADFGLARIYSCHMALTPVVVTLWYRPPEVLLQSSYATPVDIWSTGCIFAEMFRRKPLFCGDSEVDQLGKIFDVVGLPPEEEWPTDVTLSRKNFPPLLPRPITDVVPEINGQGAQLLQKMLTFDPLKRISALNALEHPYFQDEETLTQTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BHD3</accession>
    <name>A0A673BHD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Methyltransferase HEMK2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">HemK methyltransferase family member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Lysine N-methyltransferase 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methylarsonite methyltransferase N6AMT1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Methyltransferase N6AMT1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein N(5)-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">n6amt1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Methyltransferase that can methylate proteins and, to a lower extent, arsenic. Catalytic subunit of a heterodimer with TRMT112, which monomethylates 'Lys-12' of histone H4 (H4K12me1), a modification present at the promoters of numerous genes encoding cell cycle regulators. Catalytic subunit of a heterodimer with TRMT112, which catalyzes N5-methylation of Glu residue of proteins with a Gly-Gln-Xaa-Xaa-Xaa-Arg motif. Methylates ETF1 on 'Gln-185'; ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May also play a role in the modulation of arsenic-induced toxicity by mediating the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid. It however only plays a limited role in arsenic metabolism compared with AS3MT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-lysyl-[histone] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10024"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:9846"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:10025"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>methylarsonous acid + S-adenosyl-L-methionine = dimethylarsinate + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11684"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16223"/>
            <dbReference type="ChEBI" id="CHEBI:17826"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer; heterodimerization with TRMT112 is required for S-adenosyl-L-methionine-binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030014326.1">
        <property type="nucleotide sequence ID" value="XM_030158466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BHD3"/>
    <dbReference type="Ensembl" id="ENSSORT00005041732.1">
        <property type="protein sequence ID" value="ENSSORP00005040679.1"/>
        <property type="gene ID" value="ENSSORG00005018980.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435857"/>
    <dbReference type="CTD" id="29104"/>
    <dbReference type="InParanoid" id="A0A673BHD3"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036009">
        <property type="term" value="F:protein-glutamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000077">
        <property type="entry name" value="HemK methyltransferase family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="17">
        <location>
            <begin position="46"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="218" mass="23453" checksum="42AC6704B324FB89" modified="2020-06-17" version="1">MATSYPTPLYSHAGRGEFSEVYEPAEDSFLLIDALEKDAEMLQLKSPCVCLEVGSGSGVVSAFLASVVGPSALYICTDVNPAAAQCTAMTASCNGVSLQPVLTDLVECLLPWLSGKVDVLLFNPPYVVTPSEEVGSKGIEAAWAGGKRGREVTDRFLPLVAQLLSKKGLFYLVTIAENNPEEIIRLLQECGLRGESCLSTRAGNERLCILRFSWSQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIU4</accession>
    <name>A0A673BIU4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Latent-transforming growth factor beta-binding protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Forms part of the large latent transforming growth factor beta precursor complex; removal is essential for activation of complex. Interacts with LTBP1 and TGFB1. Interacts with EFEMP2; this interaction promotes fibrillar deposition of EFEMP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005042740.1">
        <property type="protein sequence ID" value="ENSSORP00005041670.1"/>
        <property type="gene ID" value="ENSSORG00005019365.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BIU4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000194">
        <property type="entry name" value="Latent transforming growth factor beta binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000205">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000019">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000046">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1 isoform x2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000056">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000017">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000115">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF4">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
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        </location>
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        </location>
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        </location>
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        </location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00064273"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00072995"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1470" mass="159934" checksum="E447C403B21DBC8A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMY7</accession>
    <name>A0A673BMY7_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <begin position="5"/>
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        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="123" mass="13439" checksum="D5F5F7531F423D25" modified="2020-06-17" version="1">MENAHTKSATEVLDNFGVNENTGLTLDQVRTNLDKYGPNGEYFITPVCPTSFHPVTLCGSIHFPGNPTVAVIFRAFLLIVGLKSCRSLWRTVRGNGTGTFTDAHACTGIIRCIKLAKRGETRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNG4</accession>
    <name>A0A673BNG4_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="term" value="F:phosphoric diester hydrolase activity"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PLCXc"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5025328062" description="Phosphatidylinositol-specific phospholipase C X domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="2">
        <location>
            <begin position="39"/>
            <end position="180"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="300" mass="34533" checksum="8EFFF9345095493E" modified="2020-06-17" version="1">IDLQLLILLLFINVSSGGFNDNRILYRSSYNLAWMKSIPDETLISAISIPGTHESLSLKGGIFTKSQVWKLDDQLRVGVRYFDVYVGIWFKHKEHIYVRDSHWMRGQDYTLDEVLKIIFDYLKAYKTETVLLKVSLYKHYKDTVLKMMEKLLEKYKSEIWTELTVPSLKQVRGKIVFLQSDTFTRGAKNEKSLFIVKNKLVDNVEEEVKKMKSRLCDRRIAVTDVITSYFDHAKKLARELNTKINDLVQKNSEDSLNRGCLGVISIDFPSVELIKKIIDLKPCQPASTESGPPSTESGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BPV8</accession>
    <name>A0A673BPV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">gria4a</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18 23">Postsynaptic cell membrane</location>
            <topology evidence="18 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BPV8"/>
    <dbReference type="Ensembl" id="ENSSORT00005045901.1">
        <property type="protein sequence ID" value="ENSSORP00005044771.1"/>
        <property type="gene ID" value="ENSSORG00005020591.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BPV8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022824">
        <property type="term" value="F:transmitter-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13727">
        <property type="entry name" value="PBP2_iGluR_AMPA_GluR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000067">
        <property type="entry name" value="glutamate receptor 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000001">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000004">
        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000666">
        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
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        <property type="entry name" value="Lig_chan"/>
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    </dbReference>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NMDARECEPTOR"/>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PBPe"/>
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        <property type="entry name" value="Periplasmic binding protein-like I"/>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
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    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="11 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 23" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 23" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 23" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 23" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 23" id="KW-0675">Receptor</keyword>
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    <keyword evidence="4 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 23" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="841"/>
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        <location>
            <begin position="438"/>
            <end position="815"/>
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    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="25">
        <location>
            <begin position="448"/>
            <end position="513"/>
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    <feature type="binding site" evidence="20">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
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        <location>
            <position position="529"/>
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        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
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        <location>
            <position position="700"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
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    <feature type="binding site" evidence="20">
        <location>
            <position position="701"/>
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        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
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        <location>
            <position position="751"/>
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        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="21">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="21">
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            <position position="679"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="21">
        <location>
            <position position="706"/>
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    </feature>
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        <location>
            <position position="798"/>
        </location>
    </feature>
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        <location>
            <begin position="106"/>
            <end position="353"/>
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    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="764"/>
            <end position="819"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SMART" id="SM00079"/>
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        <source>
            <dbReference type="SMART" id="SM00918"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="927" mass="103601" checksum="D3CDFD9C6E8345BA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BS58</accession>
    <name>A0A673BS58_9TELE</name>
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        <recommendedName>
            <fullName evidence="12">RIMS-binding protein 2</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in the synaptic transmission as bifunctional linker that interacts simultaneously with RIMS1, RIMS2, CACNA1D and CACNA1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RIMBP family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="RIMBP1/2/3"/>
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        <property type="entry name" value="PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR-ASSOCIATED PROTEIN 1"/>
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        <property type="entry name" value="SH3"/>
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    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="977"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1005"/>
            <end position="1029"/>
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    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="455"/>
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    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="1323"/>
            <end position="1391"/>
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    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="1448"/>
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        <location>
            <begin position="1513"/>
            <end position="1536"/>
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    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="180"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="21"/>
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            <begin position="36"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="15">
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1524"/>
            <end position="1536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1548" mass="168948" checksum="9AB7CD223160AC2D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTB8</accession>
    <name>A0A673BTB8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cytochrome P450 2F3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115432272</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BTB8"/>
    <dbReference type="Ensembl" id="ENSSORT00005044946.1">
        <property type="protein sequence ID" value="ENSSORP00005043832.1"/>
        <property type="gene ID" value="ENSSORG00005020220.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BTB8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046222">
        <property type="term" value="P:aflatoxin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11026">
        <property type="entry name" value="CYP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000010">
        <property type="entry name" value="cytochrome P450 2W1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF153">
        <property type="entry name" value="CYTOCHROME P450 2G1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="495" mass="56710" checksum="03EE76A88127B79E" modified="2020-06-17" version="1">MDFSATVILAGLIVALLWLFSVRNSRRYRLPPGPFALPIVGNLPMIDKNAPFKSILKVSEVKHETYGPVMTVYLGWQRTVVLVGYDAVKGALVDQADDFTGRGPLPFLVKATRGYGLGIGNGERWRQLRRFTLSTLRDFGMGRKGMEEWIQEESKHLRDRVNSFKGKSFNPTFLLSQTVSNVICCLVFGQRFDYDDAQFLRLLHVIAEVLKFGSSPRGQMFNIFPWIMDHLPGHQHTVFAQMEELREFIQVKIQEHKKTLDPSSPRDFIDCFLLRMDQEKDLPTTEFHHDNLVATVLNLFVAGTETTSSTIRYALSVLIKHPDIQEKMQHEIDTVIGQERCPNMEDRKSVPFTDAVIHEVQRFLDIVPFSLPHYALYDISFRGYTIPKNTVILPMLHSVLKEEKKWATPFSFNPQHFLDHNGNFKKNPAFMPFAAGKRACVGESLARMELFIFIVTLLQHFTFSCSGGPDTINLIPEYSSFANVPRRYELMAKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTE5</accession>
    <name>A0A673BTE5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box protein B1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115410604</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029978188.1">
        <property type="nucleotide sequence ID" value="XM_030122328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BTE5"/>
    <dbReference type="Ensembl" id="ENSSORT00005047273.1">
        <property type="protein sequence ID" value="ENSSORP00005046116.1"/>
        <property type="gene ID" value="ENSSORG00005021128.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410604"/>
    <dbReference type="InParanoid" id="A0A673BTE5"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20043">
        <property type="entry name" value="FH_FOXB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000082">
        <property type="entry name" value="forkhead box protein B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047389">
        <property type="entry name" value="FOXB1_B2_FH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF209">
        <property type="entry name" value="FORKHEAD BOX PROTEIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="273"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="316" mass="34112" checksum="02D99ED0E81DF798" modified="2020-06-17" version="1">MPRPGRNTYSDQKPPYSYISLTAMAIQSCPEKMLPLSDIYKFIMDRFPYYRENTQRWQNSLRHNLSFNDCFIKIPRRPDQPGKGSFWALHPNCGDMFENGSFLRRRKRFKVGGVQPPDPLVPTKPPDAAHYLQQQQAKLRLSALHAAAHLPQVPSGYNLGSVAPPSGFKHPFAIESIIAREYKMPGGLAAFSATGYPLHNQLTPAWPHVYGSGMMDSAAPISMATGDYSAYGMPLKSICHGGQTLPAIPVPIKATPASMPGLPGLPAHIPAFLANSPQSLSPTSPQTATGQSSPAAPGEPMTASASSGLQAAVAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BW09</accession>
    <name>A0A673BW09_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial</fullName>
            <ecNumber evidence="6">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">E2K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115415541</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="6"/>
    <dbReference type="RefSeq" id="XP_029984998.1">
        <property type="nucleotide sequence ID" value="XM_030129138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BW09"/>
    <dbReference type="Ensembl" id="ENSSORT00005046468.1">
        <property type="protein sequence ID" value="ENSSORP00005045319.1"/>
        <property type="gene ID" value="ENSSORG00005020286.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415541"/>
    <dbReference type="InParanoid" id="A0A673BW09"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000033">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="20">
        <location>
            <begin position="72"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="154"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="448" mass="48226" checksum="95432D513E411117" modified="2020-06-17" version="1">MLSRSHCVYRTLGRSLSAVNQANNVLVRQSVSGLSVCSRPAYRNSQPCEFPSAANIFHIRYFKTSAVHRDEVVTVKTPAFAESVSEGDVRWEKAVGDSVTEDEVVCEIETDKTSVQVPAPAAGVIEELLVPDGGKVEGGTPLFKLRKGAGAAKAAPSPAVEAPAAAPPSPPPPPPPPTPTPAAMPPVPPQAPKAQPVSAVKPTHAAPGPSAAGSRGESRVKMNRMRLRIAQRLKEAQNTCAMLTTFNEVDMSNIQEMRKFHKDAFLKKHNIKLGFMSAFVKAAAHALTDQPAVNAVIDDTTKEIIYRDYVDISVAVATPKGLVVPVIRNVETMNFTDIEKAINALGEKARKNELAVEDMDGGTFTISNGGVFGSLFGTPIINPPQSAILGMHGIFDRPMAINGKVEIRPMMYVALTYDHRLVDGREAVTFLRKIKSVVEDPRVLMLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZV4</accession>
    <name>A0A673BZV4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ldlrap1b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BZV4"/>
    <dbReference type="Ensembl" id="ENSSORT00005048454.1">
        <property type="protein sequence ID" value="ENSSORP00005047280.1"/>
        <property type="gene ID" value="ENSSORG00005021621.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BZV4"/>
    <dbReference type="OrthoDB" id="9999955at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13159">
        <property type="entry name" value="PTB_LDLRAP-mammal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000137">
        <property type="entry name" value="Low density lipoprotein receptor adapter protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF35">
        <property type="entry name" value="LOW DENSITY LIPOPROTEIN RECEPTOR ADAPTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="6" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="PID" evidence="9">
        <location>
            <begin position="44"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="312" mass="34259" checksum="A4E5E5AC228017A0" modified="2020-06-17" version="1">MDALKSAGRAIIRSPSIAKQSWGAGRHKKLPENWTDTRETLLEGMVFQLKYLGVTMVEQPKGEELSAAAVKRIVATAKASGKKLQKVTLTVSPRGIILYDSASNQLIENISIYRISYCTADKMHDKVFAYIVQSQHNETLECHAFLCTKRKMAQAVTLTVAQAFRVAFEFWQAAKEEKEKRVKLGSDGEGASNSQSESSASLASLKGGEVATGKLLDLAEGANVALVHSGTKQTESDPFMVHNHATENNNTVWELEDGLDEAFSRLAESRTNPQVLDIGVNPQDFNTEECLSPGKWDQEDTDFTAQKDSVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1C8</accession>
    <name>A0A673C1C8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C1C8"/>
    <dbReference type="Ensembl" id="ENSSORT00005047898.1">
        <property type="protein sequence ID" value="ENSSORP00005046732.1"/>
        <property type="gene ID" value="ENSSORG00005021382.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1C8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1068" mass="120435" checksum="F82D87766B97042D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1W1</accession>
    <name>A0A673C1W1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115413650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C1W1"/>
    <dbReference type="Ensembl" id="ENSSORT00005050440.1">
        <property type="protein sequence ID" value="ENSSORP00005049241.1"/>
        <property type="gene ID" value="ENSSORG00005022369.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1W1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042866">
        <property type="entry name" value="SUSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46839:SF1">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46839">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025427957" description="Sushi domain-containing protein" evidence="5">
        <location>
            <begin position="39"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="39"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="116"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="167"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="244"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="417"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="482" mass="52366" checksum="D4147392DFED1C93" modified="2020-06-17" version="1">MCNGMIKSVSKACWAHISVTKLPFLLLVVLAVVPTGQGSGCVRPYMVQNSWVNLTETNRGLFPVGTVLQYSCDPGYQPDGPSILTCTTLGLWSSEPPRCIRSDVCRPLSEPQNGGYICHPSPCRMFTHGTVIEFFCDEGFILKGDYKYLACQDGQWDGPAQISCVSQGCIRPFLVPHGSTNLTETNRGSFPVGTVLEYRCDPGYQPDGPTILTCTALKEWSSEPPRCIRSDVCQAPYQPENGGYTCHPSPCRRFSHGTVIEYFCDEGYILKGDYKYLTCQYGEWDNLVQISCLMEQDHDPTLPLGMPALSIVASTASSVALILLLVVLLVLLQPKLKSLHRRDQGVSGQPVSIMVEGVQVTLPSYEEAVSGGVSTASALSSESRVQIVLSEGQHATAPEAGPSRPSSFKQQHSEMDVYQPIPPTSSSQSPSPSSSTWGLEHAGAAAPLSSQRRLSAGSNQYSLPLFDSEMDYSDDMPLLKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6F4</accession>
    <name>A0A673C6F4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ras homolog family member V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115413303</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C6F4"/>
    <dbReference type="Ensembl" id="ENSSORT00005049629.1">
        <property type="protein sequence ID" value="ENSSORP00005048437.1"/>
        <property type="gene ID" value="ENSSORG00005022095.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C6F4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04130">
        <property type="entry name" value="Wrch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000561">
        <property type="entry name" value="rho-related GTP-binding protein RhoV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="230" mass="26162" checksum="762060FFDB4FE754" modified="2020-06-17" version="1">SDYVNLHQKETIMLGGLHLIGRFLSCMLVGDGAVGKTSMIISYIFNGYNSEYRQTAFDVFTGLVHVNGVPTRIKLIDTAGQEEFGHLRSLCYAHVDVFILCFSLVNPVSFDNITSKWIPQIRARNLTSPIVLVGTQSDLCHDVDVLIHLDQRNSRPVHLRQARRLAKRIRAHDYMECSALTQHNLKDVFDCAISAAMKQKHTGTKSKKLSLFKRLKTFSYCGWRKVFQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C8T5</accession>
    <name>A0A673C8T5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Solute carrier family 41 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">slc41a2b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Acts as a plasma-membrane magnesium transporter. Can also mediate the transport of other divalent metal cations in an order of Ba(2+) &gt; Ni(2+) &gt; Co(2+) &gt; Fe(2+) &gt; Mn(2+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Co(2+)(in) = Co(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28578"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Mn(2+)(in) = Mn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28699"/>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ni(2+)(in) = Ni(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29831"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C8T5"/>
    <dbReference type="FunCoup" id="A0A673C8T5">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050867.1">
        <property type="protein sequence ID" value="ENSSORP00005049664.1"/>
        <property type="gene ID" value="ENSSORG00005022495.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C8T5"/>
    <dbReference type="OrthoDB" id="5791097at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000001">
        <property type="entry name" value="Solute carrier family 41 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000002">
        <property type="entry name" value="Solute carrier family 41, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF25">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="268"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="467"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="537"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="19">
        <location>
            <begin position="196"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="19">
        <location>
            <begin position="410"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU369007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="569" mass="60819" checksum="BCD148F9E2848014" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAS5</accession>
    <name>A0A673CAS5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDAN1-interacting nuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cdin1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CAS5"/>
    <dbReference type="FunCoup" id="A0A673CAS5">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051603.1">
        <property type="protein sequence ID" value="ENSSORP00005050394.1"/>
        <property type="gene ID" value="ENSSORG00005022850.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CAS5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029404">
        <property type="entry name" value="CDIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661:SF1">
        <property type="entry name" value="CDAN1-INTERACTING NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661">
        <property type="entry name" value="SIMILAR TO CDNA SEQUENCE BC052040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14811">
        <property type="entry name" value="TPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="304" mass="35234" checksum="591EA5F078225C90" modified="2020-06-17" version="1">REAADGELSTDTASDPRKMRLSRAEYKQIVRWTEQQRPTRQCMKVLKDKFPNHSQSTLLSIFSLEYQKRTKRTISRHHAPDVIESYYQRYCIIAVSIASYSLCFFSHLYHCVDLSPALMARLILDRFLLDLEGGMPSKTILNSMLKEPSLIPDQILAKHIYQCTINDCCYGPLVDCIKHAIGQEHEVLLCDKLKERNLSFLDENQLRAMGYDKTPDIILEVPVAVEGHIVHWIESKASFGDDHSHRTYLNEQFWSYWNRFGPGLVIYWYGFIGELDCQRDRGILLKDCFPTDIVTLCHAAQQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBN2</accession>
    <name>A0A673CBN2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CCN family member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">si:ch211-106h11.3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBN2"/>
    <dbReference type="FunCoup" id="A0A673CBN2">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054225.1">
        <property type="protein sequence ID" value="ENSSORP00005052971.1"/>
        <property type="gene ID" value="ENSSORG00005023866.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBN2"/>
    <dbReference type="OrthoDB" id="365605at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000015">
        <property type="entry name" value="CYR61 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012395">
        <property type="entry name" value="IGFBP_CNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF20">
        <property type="entry name" value="PROTEIN CYR61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036495">
        <property type="entry name" value="IGFBP_rP_CNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025598273" evidence="7">
        <location>
            <begin position="21"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="10">
        <location>
            <begin position="18"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="9">
        <location>
            <begin position="94"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="8">
        <location>
            <begin position="300"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="396" mass="43831" checksum="91C78A9A3571EEA5" modified="2020-06-17" version="1">MVILLLFAVLQVMTIGSVSAGCPVVCECPAGPPSCPPGVSSVPDSCGCCKVCAAQLNQDCHEGRPCDHHKGLECNYGNDVGRTHGICRAKAEGRSCEYNGRIYQNGENFRAGCKHQCTCIDGAVGCVPLCPTHVPLASPSCPAPQLVKVPGQCCLSIDCHKGTTIVPPVHRRPQPPAYPPYPFIPYPAYPYLKPYSKPYRKLYPYKPKKEKDNLGNELVEVGRKWDKPRGHNHLAAWRQVGDQCVVQTTSWSQCSRSCGMGVSSRVTNDNARCKLIKETRLCNIRPCSSMSIPVKKGRKCSRTHKAPEPHRLSYAGCRSTRLYRPNYCGVCRDGRCCSPRRTRTASVTFACPDGERFNRSVMFIQSCKCSDECNHLNEAAMPPQRWLYGDTHKFMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGU2</accession>
    <name>A0A673CGU2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="14">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Long-chain-fatty-acid--CoA ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115429869</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A673CGU2"/>
    <dbReference type="Ensembl" id="ENSSORT00005056077.1">
        <property type="protein sequence ID" value="ENSSORP00005054801.1"/>
        <property type="gene ID" value="ENSSORG00005024479.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGU2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000002">
        <property type="entry name" value="Long-chain fatty acid transport protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000005">
        <property type="entry name" value="Solute carrier family 27 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006134">
        <property type="entry name" value="PRK08279.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF16">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="18">
        <location>
            <begin position="65"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="19">
        <location>
            <begin position="499"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="623" mass="70304" checksum="FEE6977E58E88521" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLI8</accession>
    <name>A0A673CLI8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nol10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat NOL10/ENP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029983382.1">
        <property type="nucleotide sequence ID" value="XM_030127522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CLI8"/>
    <dbReference type="FunCoup" id="A0A673CLI8">
        <property type="interactions" value="1989"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054136.1">
        <property type="protein sequence ID" value="ENSSORP00005052883.1"/>
        <property type="gene ID" value="ENSSORG00005023831.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414250"/>
    <dbReference type="CTD" id="79954"/>
    <dbReference type="InParanoid" id="A0A673CLI8"/>
    <dbReference type="OrthoDB" id="273340at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001909">
        <property type="entry name" value="WD repeat, SAM and U-box domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056551">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040382">
        <property type="entry name" value="NOL10/Enp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056550">
        <property type="entry name" value="NOL10_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23098">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23097">
        <property type="entry name" value="NOL10_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Nucleolar protein 10-like N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolar protein 10-like second" evidence="9">
        <location>
            <begin position="369"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="8">
        <location>
            <begin position="483"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="525"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="555"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="621"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="672"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="719" mass="83133" checksum="99724A9C25AD1B7E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMQ1</accession>
    <name>A0A673CMQ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">D-beta-hydroxybutyrate dehydrogenase, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bdh1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CMQ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005055885.1">
        <property type="protein sequence ID" value="ENSSORP00005054609.1"/>
        <property type="gene ID" value="ENSSORG00005024449.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CMQ1"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09805">
        <property type="entry name" value="type2_17beta_HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000074">
        <property type="entry name" value="Retinol dehydrogenase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF36">
        <property type="entry name" value="D-BETA-HYDROXYBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="359" mass="40085" checksum="C30BCCCD9C6E9225" modified="2020-06-17" version="1">MAPALRVVLLVSFSVFLTVLLGFGLPALLNASMRLLGLPETSVTECIVVMYLFFVLYVATPRIPRGLVEVKGKAVFVTGCDSGFGNALAKHLHKLGFTVFAGCFLKDKGGEGAKELEEFHSDRMKVVQLDVCSEEQVKEAVEYIKDNLEDSERGLWAVVNNAGVSTFGEVEFTSMDTYKQVSEVNLWGTIRVTKAVLPLIRRAKGRVVNLASMYGRMGNVMRSPYCVSKYGVEAFSDCLRYEMKTWGVKVSVVEPGNFIVATGILTRDIVASTANKLWNEAPSDVKEDYGKAHFEHLMALMRSYCNSGQKEVTPVLDDITDAIMSKRPYTRYNPMEPHWWIRVQLMTHLPGAISDFLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQV8</accession>
    <name>A0A673CQV8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acetylserotonin O-methyltransferase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">asmtl</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CQV8"/>
    <dbReference type="Ensembl" id="ENSSORT00005057020.1">
        <property type="protein sequence ID" value="ENSSORP00005055734.1"/>
        <property type="gene ID" value="ENSSORG00005024851.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CQV8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000358">
        <property type="entry name" value="Acetylserotonin O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000146">
        <property type="entry name" value="Acetylserotonin O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000020">
        <property type="entry name" value="Probable bifunctional dTTP/UTP pyrophosphatase/methyltransferase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="9">
        <location>
            <begin position="352"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="8">
        <location>
            <begin position="455"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="682" mass="75982" checksum="5D158CF311D40C93" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSE8</accession>
    <name>A0A673CSE8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">stk39</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673CSE8"/>
    <dbReference type="Ensembl" id="ENSSORT00005059907.1">
        <property type="protein sequence ID" value="ENSSORP00005058581.1"/>
        <property type="gene ID" value="ENSSORG00005025817.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSE8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010820">
        <property type="term" value="P:positive regulation of T cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000043">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000068">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000562">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF14">
        <property type="entry name" value="STE20_SPS1-RELATED PROLINE-ALANINE-RICH PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="57"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="356"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="533" mass="59560" checksum="E682276D323F671B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CTU2</accession>
    <name>A0A673CTU2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA damage-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Damage-specific DNA-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ddb1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DDB1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CTU2"/>
    <dbReference type="Ensembl" id="ENSSORT00005059134.1">
        <property type="protein sequence ID" value="ENSSORP00005057815.1"/>
        <property type="gene ID" value="ENSSORG00005025586.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CTU2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.910:FF:000001">
        <property type="entry name" value="DNA damage-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000073">
        <property type="entry name" value="DNA damage-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002576">
        <property type="entry name" value="DNA damage-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002484">
        <property type="entry name" value="DNA damage-binding protein 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="15">
        <location>
            <begin position="14"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="16">
        <location>
            <begin position="396"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="14">
        <location>
            <begin position="790"/>
            <end position="1099"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1140" mass="126410" checksum="5F62EA52E5A28253" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CV01</accession>
    <name>A0A673CV01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Neugrin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Neurite outgrowth-associated protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ngrn</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an essential role in mitochondrial ribosome biogenesis. As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation of core subunits of the oxidative phosphorylation system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a regulatory protein-RNA complex, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA. Interacts with 16S mt-rRNA; this interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the neugrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029995849.1">
        <property type="nucleotide sequence ID" value="XM_030139989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CV01"/>
    <dbReference type="Ensembl" id="ENSSORT00005058609.1">
        <property type="protein sequence ID" value="ENSSORP00005057294.1"/>
        <property type="gene ID" value="ENSSORG00005025424.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423219"/>
    <dbReference type="CTD" id="51335"/>
    <dbReference type="InParanoid" id="A0A673CV01"/>
    <dbReference type="OrthoDB" id="6415470at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010487">
        <property type="entry name" value="NGRN/Rrg9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475:SF4">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06413">
        <property type="entry name" value="Neugrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025584231" description="Neugrin" evidence="18">
        <location>
            <begin position="17"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="302" mass="33672" checksum="6BD7161893547C34" modified="2020-06-17" version="1">MARPLQLLSLLSRVGALSPMPSTTFTGRRFVSKGVTRTWNGLTQRDRASTYGDEEVYEGGDELEDVEDKLQALLNEGRKRQKTVKYHILRRKMTPSGAPERSLTWDAIEQIRYLKAEQPEEWTVKRLAEGFSVSPDVILRVLRTKFIPSPERKAKQDTKVMVALNQQTLPSGVVKVQERPRLTGNSAAAVLPPGNTENTVVPVENQTLTLRGEGSGSLTVSPVPVAALGRLSAADTSKHTLVRSTKEENATSRVTEEEEESWDGQVLTEEQLEQCMETVTFSPVVQVGKDFFDVEGKFLYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CZB1</accession>
    <name>A0A673CZB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apolipoprotein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">apod</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CZB1"/>
    <dbReference type="Ensembl" id="ENSSORT00005059660.1">
        <property type="protein sequence ID" value="ENSSORP00005058337.1"/>
        <property type="gene ID" value="ENSSORG00005025754.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CZB1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19437">
        <property type="entry name" value="lipocalin_apoD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000003">
        <property type="entry name" value="Apolipoprotein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026222">
        <property type="entry name" value="ApoD_vertbrte"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002969">
        <property type="entry name" value="ApolipopD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF34">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02058">
        <property type="entry name" value="APODVERTBRTE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01219">
        <property type="entry name" value="APOLIPOPROTD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="11">
        <location>
            <begin position="38"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="194" mass="21920" checksum="84E30092EBD5966D" modified="2020-06-17" version="1">MFDTPGLTNKDPNIHWQPKSSTDINCLIPIDPLILSIHYMGTWYEIEKLPAAFERGECVQATYSRLPDGMVKVYNEELLPDGKINSIEGVAEVKDPSQPAILSVSFFKGNIVIVFCSPYWVLSTDYKSYSLVYSCSDYLSLFHIDFAWILARTRVLSEDFVNQLRGKLEAAGVNIKQLTVTNQTDCAGHHTFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4B9</accession>
    <name>A0A672Y4B9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115424568</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y4B9"/>
    <dbReference type="Ensembl" id="ENSSORT00005001181.1">
        <property type="protein sequence ID" value="ENSSORP00005001147.1"/>
        <property type="gene ID" value="ENSSORG00005000692.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4B9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000001">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000008">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008557">
        <property type="entry name" value="PRK11493.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="48"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="197"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="320" mass="35336" checksum="B393B7970836AB36" modified="2020-06-17" version="1">MKGLKGPFSECCPLVAGNGTSPRFIMAAQTRALVSAQWLADALKSNLVGPKLRILDTSWYLPKTKRDTKAEFAQAHIPGSSFFDIDECSDHSSAYPHMLPTSTHFSEYVGELGIGNDTHVVVYDTSSFGSYSAPRVWWMFRVFGHSLVSVLDGGMKNWKAEGQAVTSEYSKPDRTQFKATFNQSWVKSYEDILENIRTKQVQVVDARSAGRFRGTEPEPRDDTLPGHFANAINMPFTSFMDESGKSVGTEDLSRLFKEAGVDLDQPLWVSCGSGVTACHVVLAAHQLGYPGACVYDGSWTEWFLRSSPEHIISEGEGKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4W3</accession>
    <name>A0A672Y4W3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-threonine 3-dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115434756</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate, mediating L-threonine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-threonine + NAD(+) = (2S)-2-amino-3-oxobutanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78948"/>
            <dbReference type="EC" id="1.1.1.103"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.103" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y4W3"/>
    <dbReference type="Ensembl" id="ENSSORT00005001157.1">
        <property type="protein sequence ID" value="ENSSORP00005001123.1"/>
        <property type="gene ID" value="ENSSORG00005000666.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4W3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008743">
        <property type="term" value="F:L-threonine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05272">
        <property type="entry name" value="TDH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000077">
        <property type="entry name" value="L-threonine 3-dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051225">
        <property type="entry name" value="NAD(P)_epim/dehydratase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42687">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42687:SF1">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="7">
        <location>
            <begin position="85"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="367" mass="41060" checksum="73F8FF828E35CE23" modified="2020-06-17" version="1">MQVGQLLRQSVLKHAGSRPLCSSEPLISAVRGISSSPQQFAASGRAHTSSNTDTDVPKVLITGGFSYIVLKRFGKNNVILSDIRKPPIDVYHNGPFIFSDILDYKNLREIVVNNNISWLVHYSAVLSAVGESNVPLAKEVNITGLHNILDISTEHGLRVFVPSTIGAFGPSSPRDPTPELCVQRPRTIYGVSKVHAELMGEYYHHRYGLDFRCLRYPGIISADSQPGGGTTDYAVQIFHSAVKTGHFECNLRSDTRLPMMYIDDCLRATLEFLEAPADKLVSRTYNIHSMSFTPHDLTQEMQKLLPYLKVTYNVDPVRQAIADSWPMALEDSAARRDWGWKHEYDLPQLVQTMLTQIITNHKLAQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6K4</accession>
    <name>A0A672Y6K4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 72 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Transcription factor-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">vps72a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Deposition-and-exchange histone chaperone specific for H2AZ1, specifically chaperones H2AZ1 and deposits it into nucleosomes. As component of the SRCAP complex, mediates the ATP-dependent exchange of histone H2AZ1/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS72/YL1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y6K4"/>
    <dbReference type="Ensembl" id="ENSSORT00005002271.1">
        <property type="protein sequence ID" value="ENSSORP00005002212.1"/>
        <property type="gene ID" value="ENSSORG00005001346.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y6K4"/>
    <dbReference type="OrthoDB" id="78296at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046757">
        <property type="entry name" value="YL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 72 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275">
        <property type="entry name" value="YL-1 PROTEIN TRANSCRIPTION FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05764">
        <property type="entry name" value="YL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="6">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="129"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="367" mass="41590" checksum="1802FBCEA72910AC" modified="2020-06-17" version="1">MSLVVGREPRKTAGNRMSKLLDAEEEDEFYKTTYGGFDDESGDDEYHGEHSDTEDEVDSDFDIDEGDEPDSDQEEDAPRRKGRVVTKAYKEPIKVAKPKPKRPSEEQKKTEKVKVELKRRIPQEFQDFGETRKSVRQSTSEHTRKTNLRLQERQDAPRRRRGAHRDRPLTQEELLAEAKITAEINIRSLENYERLEADKKKQVHKKRRFEGPTIRHHSVLMPIVSHSVLKEENVDVEGLDQDVPQTAQTNPTTPSQQPAGGLCSRTYITFSDDEAFEAAFPNSAQSSLQLPVQEICPVTHKAALYRDPVTDIPYANTRAFRIIREAYRKYVAAHGFPNTSGGASGLDSSAPKSARQKMVVKQAAVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y7P1</accession>
    <name>A0A672Y7P1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine zipper transcription factor-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lztfl1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Regulates ciliary localization of the BBSome complex. Together with the BBSome complex, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May play a role in neurite outgrowth. May have tumor suppressor function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Self-associates. Interacts with BBS9; the interaction mediates the association of LZTL1 with the BBsome complex and regulates BBSome ciliary trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LZTFL1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y7P1"/>
    <dbReference type="Ensembl" id="ENSSORT00005002581.1">
        <property type="protein sequence ID" value="ENSSORP00005002518.1"/>
        <property type="gene ID" value="ENSSORG00005001494.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y7P1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903565">
        <property type="term" value="P:negative regulation of protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026157">
        <property type="entry name" value="LZTFL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21635">
        <property type="entry name" value="LEUCINE ZIPPER TRANSCRIPTION FACTOR LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21635:SF0">
        <property type="entry name" value="LEUCINE ZIPPER TRANSCRIPTION FACTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15294">
        <property type="entry name" value="Leu_zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="90"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="190"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="242" mass="28142" checksum="FC212ED6C28D6263" modified="2020-06-17" version="1">MLDDLQAVVRGEVETELINTAHTNVLLLRQLFSQAEKFYLRLQTDISELENRELLEQVAEFEKTDFKTPDKMNQETSKPKLAPLNEGGVSELLNKEITRLQEENDKLKSRLRTLETQAMSALDEKTKAERALKDLQKVQSEHQMMAHSQEITSLEDTVAALKDDYERSLSANAASQKDLQENLISSKHELLRVQEQLTLAEKELEKKFQQTAAYRNMKEILTKKNEQIKEIRKRLQRYEPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y7Z9</accession>
    <name>A0A672Y7Z9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein arginine N-methyltransferase 3</fullName>
            <ecNumber evidence="3">2.1.1.319</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">prmt3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="3"/>
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        <property type="protein sequence ID" value="ENSSORP00005002822.1"/>
        <property type="gene ID" value="ENSSORG00005000789.1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Protein arginine N-methyltransferase 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="protein arginine N-methyltransferase 3 isoform X2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ANM3-like_C2H2_Zf"/>
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        <property type="entry name" value="Arg_MeTrfase"/>
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        <property type="entry name" value="Methyltransf_25"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
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        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
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    </dbReference>
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        <property type="entry name" value="SAM_MT_PRMT"/>
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    <keyword evidence="6 18" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="8 18" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
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        </location>
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        </location>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022603"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022691"/>
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        </source>
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        <source>
            <dbReference type="Pfam" id="PF13649"/>
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            <dbReference type="Pfam" id="PF21137"/>
        </source>
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        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSSORP00005002822.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="510" mass="57856" checksum="62C70B91910B4846" modified="2020-06-17" version="1">MPELSDPDEEEEDDEEQWQWMEEENQPVTCLFCDRLLSSVSATLQHCTAEHQLNLIDLIRKHNLDDYGYIKMINFIRSTNCDASGLMGLPDGSLPWDSEAFLRPVLQDDPLLQTDPEELCGQEGSLHQSSRSESHDALLQRTQAAEERAQRSEEALARAMEDLHKLKLLAQGLVLNAEPGRSGNLSAVAELREDEDEAYFDSYGHYGIHEEMLKDKVRTESYRDFMYRNPEVFRDKVVLDVGCGTGILSMFAAKAGAKKVIAVDQSEIIYQAMDIVRSNQLEDKITLIKGRIEDINLPVEKVDIIISEWMGYFLLFESMLDSVLYARDLYLADGGSVYPDLCNISLAAAGDMQRHQERIAFWDNVYGFNMACMKKAVVPEAVVEVVKAETLISEPTVIQTIDCNRVCLSELEFVSDFCLKIKDTTDCTAIVGYFDIFFDKGCGNKVMFSTGPQVMKTHWKQTVFLLEKPLSVQAGEELQGKITVRKNKKDPRSLLVTFDLRDRKQTYSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF78</accession>
    <name>A0A672YF78_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cdc42 effector protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115423378</name>
        <name evidence="5" type="synonym">cdc42ep4a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORG/CEP family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030140142.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSSORT00005003448.1">
        <property type="protein sequence ID" value="ENSSORP00005003349.1"/>
        <property type="gene ID" value="ENSSORG00005002043.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003454.1">
        <property type="protein sequence ID" value="ENSSORP00005003355.1"/>
        <property type="gene ID" value="ENSSORG00005002045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423378"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:cytoskeleton"/>
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    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:small GTPase binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of actin filament polymerization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of pseudopodium assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:Rho protein signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Cdc42_effect-like"/>
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        <property type="entry name" value="Cdc42_Effector_BORG/CEP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344:SF19">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN (RHO GTPASE BINDING) 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN BORG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14957">
        <property type="entry name" value="BORG_CEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="4">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="45"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="212"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="293"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="358" mass="39314" checksum="658903A655147D20" modified="2020-06-17" version="1">MPILKQLVSGSSQTKRRSRMDLTREMISAPLGDFRHTMHVGRSGDAFGDTSFLSTRSGEPPSETTSFPRSPRPGLLSRTFRSSKRSQSVTRVDQQKDDSLVVAGGSPTYVKNAMSLPFLNDEDRGDDIVSKSMSSSPLNHHHRVEVDGKGAAAASYLMELDERNFGELTELPESSHHFGGGMKHAESVMSFHVDLGPSMLGDILGVMEKEEDDLGYEEGKSSEDRDSPPLSTHGEEEDGGDKEDRTRQEEEDEEDDDDDDEEAAELQPPSTHPPSSFDLGPENGGPYTPEYTPETRPKHLQHLEDSCSMSSSGSAAFDEKPNSQTYAGDTDSATFSAPPEEESNFSSFLEDEDDEIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFA1</accession>
    <name>A0A672YFA1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YFA1"/>
    <dbReference type="Ensembl" id="ENSSORT00005003535.1">
        <property type="protein sequence ID" value="ENSSORP00005003435.1"/>
        <property type="gene ID" value="ENSSORG00005002073.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFA1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF151">
        <property type="entry name" value="ICOS LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="28"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="159" mass="17355" checksum="9C425D5F28139D3B" modified="2020-06-17" version="1">MRTNTDTGHRSSAGPSPAASAATMHLRPGFSLLLCATVVHMAPVPDPQVSAETGQNVTLQCRGPPDPAMVKWVRPDLERDGYVLYVQGRIRPSYHHEAYKNRVEPLDPSMKNGVYSVVLMNVSSSDNGTYKCVIGMQGQEPTEQKVHLQVSGELLPLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YG18</accession>
    <name>A0A672YG18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nondiscriminating glutamyl-tRNA synthetase EARS2, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
            <ecNumber evidence="10">6.1.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glutamate--tRNA(Gln) ligase EARS2, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ears2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Gln) + L-glutamate + ATP = L-glutamyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:64612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:64613"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:23541"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Glx) + L-glutamate + ATP = L-glutamyl-tRNA(Glx) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9713"/>
            <dbReference type="Rhea" id="RHEA-COMP:9716"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:18398"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EC" id="6.1.1.24" evidence="10"/>
    <dbReference type="RefSeq" id="XP_029982852.1">
        <property type="nucleotide sequence ID" value="XM_030126992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YG18"/>
    <dbReference type="FunCoup" id="A0A672YG18">
        <property type="interactions" value="799"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003617.1">
        <property type="protein sequence ID" value="ENSSORP00005003516.1"/>
        <property type="gene ID" value="ENSSORG00005002089.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413843"/>
    <dbReference type="CTD" id="124454"/>
    <dbReference type="InParanoid" id="A0A672YG18"/>
    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050561">
        <property type="term" value="F:glutamate-tRNA(Gln) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="18">
        <location>
            <begin position="46"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="19">
        <location>
            <begin position="398"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="551" mass="62961" checksum="EAB0DFDC933FBA68" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIK1</accession>
    <name>A0A672YIK1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Alkylated DNA repair protein alkB homolog 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">alkbh7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro), but this activity may be due to the absence of the true substrate. Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death. ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage. Does not display DNA demethylase activity. Involved in fatty acid metabolism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997738.1">
        <property type="nucleotide sequence ID" value="XM_030141878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YIK1"/>
    <dbReference type="Ensembl" id="ENSSORT00005004542.1">
        <property type="protein sequence ID" value="ENSSORP00005004410.1"/>
        <property type="gene ID" value="ENSSORG00005002674.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424540"/>
    <dbReference type="CTD" id="84266"/>
    <dbReference type="InParanoid" id="A0A672YIK1"/>
    <dbReference type="OrthoDB" id="28127at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000009">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032870">
        <property type="entry name" value="ALKBH7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21052:SF0">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21052">
        <property type="entry name" value="SPERMATOGENESIS ASSOCIATED 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1210">Necrosis</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="234" mass="26636" checksum="2973B584A26FB616" modified="2020-06-17" version="1">MKLLLAVVTRLNKPSFWTQCVRCQSSGANSGLLPLADPALIVGSSRELVQRLGPQVEVRTGFITEEEEGAFLRELEPGLKKKRYEFDHWDDAIHGYRETERVRWGEACEEILNRVRSVAFVEGSPLLGPVHVLDLDKSGYIKPHIDSVKFCGSTIAGLSLLSDSIMRLVKEDDASERLDLLLPRYSLYILRDQARYNFTHEILKDEESVFNGQKIPRQRRISVICRNLPGQHPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLC8</accession>
    <name>A0A672YLC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SoHo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLC8"/>
    <dbReference type="Ensembl" id="ENSSORT00005005563.1">
        <property type="protein sequence ID" value="ENSSORP00005005405.1"/>
        <property type="gene ID" value="ENSSORG00005003208.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLC8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003127">
        <property type="entry name" value="SoHo_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50831">
        <property type="entry name" value="SOHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SoHo" evidence="3">
        <location>
            <begin position="118"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="166" mass="18149" checksum="1846B161F95E9454" modified="2020-06-17" version="1">MNTERLYNGGCARKSVALSLILSPMKRVQSSPNLATAYWRCFDVTDGLKNGDASSSSLAAKGFRSVRPNLQDKKSPTQVKILEFVYSVTRESVVSGHTSVSSTVPIARFTEEEKKVSVIKAPHYEGIGPVDESGIPIAIRTVRSHNTEQGRCSSCLFNHDIISECN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLE8</accession>
    <name>A0A672YLE8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YLE8"/>
    <dbReference type="Ensembl" id="ENSSORT00005005584.1">
        <property type="protein sequence ID" value="ENSSORP00005005426.1"/>
        <property type="gene ID" value="ENSSORG00005003226.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLE8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="67" mass="7632" checksum="547B263BE1906873" modified="2020-06-17" version="1">IKNAIIEHFWLLNLLSQMKDFEASAEPLQECLNATELAVQESSTRLHDLTAKKLELHKLQVPTGVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YME3</accession>
    <name>A0A672YME3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein cramped-like</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cramped chromatin regulator homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Hematological and neurological expressed 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cramp1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cramped family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YME3"/>
    <dbReference type="Ensembl" id="ENSSORT00005006038.1">
        <property type="protein sequence ID" value="ENSSORP00005005791.1"/>
        <property type="gene ID" value="ENSSORG00005002684.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YME3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000439">
        <property type="entry name" value="Cramped chromatin regulator homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055315">
        <property type="entry name" value="Cramped-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21677">
        <property type="entry name" value="CRAMPED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21677:SF1">
        <property type="entry name" value="PROTEIN CRAMPED-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="9">
        <location>
            <begin position="177"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRA8</accession>
    <name>A0A672YRA8_9TELE</name>
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            <fullName evidence="5">Coagulation factor IX</fullName>
            <ecNumber evidence="4">3.4.21.22</ecNumber>
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        <alternativeName>
            <fullName evidence="22">Christmas factor</fullName>
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        <name evidence="30" type="primary">f9a</name>
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    <organism evidence="30 31">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
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    <reference evidence="30" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.</text>
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        </reaction>
    </comment>
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        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="504" mass="56506" checksum="C1B065E93F76D320" modified="2020-06-17" version="1">MAPFLWFSLCLLLLDFQLGSGDPVFLSVQAANSVLQRQKRENTGFFEEFLQGDLERECIEEKCDLEEARETFENDEKTMEFWAGYIDGNQCTSSPCLNQGTCKDLLRYYTCFCPPGFIGRNCEIVVARRCDVNNGDCIHFCTDAFLSSFLYSVLMTLNFYQGTFFTLPDEFPCGRTALTEIGNALRRSVFAFDESLQNNASVITISTTTPSLPTSASTTTSIPDTFDSMENETEWMYNNTDSPTGKPLRPYRRIVGGELVTPGEIPWQVALIARPSGQLFCGGSILSSLWVITAAHCLVEAQNSFFVRVGENNIYINEGTEQDYEVLQQHIHPRYNANISLFNHDIGLLYLKDPITFSSTVRPICIGPRTFTEALVKDASPALVSGWGRTRFLGAMAISLQKVEVPFTDRTTCKDSSSSRITPFMFCAGYYDEDKDACQGDSGGPHANSFHDTWFLTGIVSWGEECAQKGKYGVYTRLALYYSWIQHVMGVTHKRLALDVEDPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YS78</accession>
    <name>A0A672YS78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">zdhhc2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A672YS78"/>
    <dbReference type="FunCoup" id="A0A672YS78">
        <property type="interactions" value="1307"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007717.1">
        <property type="protein sequence ID" value="ENSSORP00005007438.1"/>
        <property type="gene ID" value="ENSSORG00005003839.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YS78"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="116"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="365" mass="43094" checksum="B5FB33C907F34F97" modified="2020-06-17" version="1">MAPSGSRSIKRGCQRVLYWIPVLFIALIVAWSYYAYVLQLCIVIYLLVYHVVFIMFVWAYWQTIFTKPMNPLKEFHLSYSDKELLEREDRGESQQEILRRIAKDLPIYTRTNSGAIRFCDRCQLLKPDRCHHCSVCDKCILKMDHHCPWVNNCVGFSNYKFFMLFLAYSLLYCLFITATDLKYFIKFWLNGLPDTQAKFHILFLFFSASMFSVSLASLFIYHCWLVCKNRSTLEAVRAPVFRHGTDKNGFSLGFSKNFRQVFGDEVKYWPAYCIKSALSLNLKGCGSLIFYDMNLVILIFTEVNVSCHWCHENVLFYNKNHTFDSISLPFTYFLKICEESHMIKSSVLFWGFFQLLKIYLRLDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTX0</accession>
    <name>A0A672YTX0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Complement factor H like 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YTX0"/>
    <dbReference type="Ensembl" id="ENSSORT00005008302.1">
        <property type="protein sequence ID" value="ENSSORP00005008019.1"/>
        <property type="gene ID" value="ENSSORG00005004490.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTX0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051503">
        <property type="entry name" value="ComplSys_Reg/VirEntry_Med"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785:SF2">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="84"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="145"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="209"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="273"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="275"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="405" mass="45443" checksum="DBEAFC81857B1AC6" modified="2020-06-17" version="1">KLVTSVVFVVNLSPLPFFFFYYLTVCSDLPKVQNGHVTEETRKTAYQGGDVIRFDCDIGYISGPTITYVCSDGTWLSVRSGRLKPCVLPDDTPNGYYEITVGADFVFGATIKYFCNEGYQMISKESTRTCGLEGWSNHLPICEPLSCDPPPTDVNVTVHGLPENEGAILPDRFLTFSCNHPGMRLNGSSRLICGTDGKWDHDFPSCEDVTCELPRIPRHLVIKELPPANETMKVGHRLEFWCDTENPSQGTREITCLQSGQWSGNFPTCSGTLDCGTPPHLTDGDIKGTVRSTYKHGERVEYMCQNVYKMEGGPYKTCRDSEWIGDIRCIQPCTVNRALMESHNIKFKYRRDNKLYAPHGDRITFSCVAGTRHDGLLDMVQFCNEGVMNLPTCLSSFQVEIINRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUR8</accession>
    <name>A0A672YUR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pxdc1b</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YUR8"/>
    <dbReference type="FunCoup" id="A0A672YUR8">
        <property type="interactions" value="974"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008623.1">
        <property type="protein sequence ID" value="ENSSORP00005008329.1"/>
        <property type="gene ID" value="ENSSORG00005004629.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YUR8"/>
    <dbReference type="OrthoDB" id="9933228at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040288">
        <property type="entry name" value="PXDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31433">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31433:SF0">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="47"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="233" mass="27341" checksum="CD3032B3A86EDBF9" modified="2020-06-17" version="1">MASAVFEGTSLVNMFVRDCWVNGIRRLIISQRGEEEEFFEIRTEWSDRNILYLHRSYADLGRLFKRLVESFPEDRRDLSKCPLIEGLVKIKEAHDIEEKLNEVERLLKNAINMPCKYSRSEVMLTFFERSPLDQVLRNDKVHRIQPCFQSPIKISEIMRSNGFCLANTETIVFDHNLEEEKERPSSTDSAEHTYEDGTEFLPLEADVCDEETEAYVTNLSYYHLVPFETDILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YVJ5</accession>
    <name>A0A672YVJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">commd2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029988920.1">
        <property type="nucleotide sequence ID" value="XM_030133060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YVJ5"/>
    <dbReference type="FunCoup" id="A0A672YVJ5">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008874.1">
        <property type="protein sequence ID" value="ENSSORP00005008574.1"/>
        <property type="gene ID" value="ENSSORG00005004752.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418550"/>
    <dbReference type="CTD" id="51122"/>
    <dbReference type="InParanoid" id="A0A672YVJ5"/>
    <dbReference type="OrthoDB" id="10257479at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd04750">
        <property type="entry name" value="Commd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037354">
        <property type="entry name" value="Commd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15857">
        <property type="entry name" value="COMM DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15857:SF0">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="123"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="198" mass="22318" checksum="9D999A56E5DE8C65" modified="2020-06-17" version="1">MLLVLSEEHKDHLGFLPKVPTAVVAEFGRIALEFLRKGTSPKIYEGAARKLCVPVDVVQHGVEGLMYLMLESSKHMISEVDFLDSVLVLGFNEDLNQTLLQLYLQHHSQIRSVVNQLPFNPPSYHNLEWRLDVQLASRSLRQQLIPVLTVQLLLSGHSNSSQVLQTDPSTLLHLISTLETALTAMKSSHARRILRNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXK5</accession>
    <name>A0A672YXK5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Deoxyribose-phosphate aldolase</fullName>
            <ecNumber evidence="5">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphodeoxyriboaldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">dera</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate. Participates in stress granule (SG) assembly. May allow ATP production from extracellular deoxyinosine in conditions of energy deprivation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with YBX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DeoC/FbaB aldolase family. DeoC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672YXK5"/>
    <dbReference type="Ensembl" id="ENSSORT00005009561.1">
        <property type="protein sequence ID" value="ENSSORP00005009255.1"/>
        <property type="gene ID" value="ENSSORG00005004943.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YXK5"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046386">
        <property type="term" value="P:deoxyribose phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000103">
        <property type="entry name" value="Putative deoxyribose-phosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF3">
        <property type="entry name" value="DEOXYRIBOSE-PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="324" mass="35767" checksum="9B3092C405537A26" modified="2020-06-17" version="1">MVRWRSETGPAAHILIRISKVRVNTQAVLKRAQQIQSMKTPKKQWQAAWLLKAVTCIDLTTLAGDDTPSNVHRLCLKAIQPVRYDLLKSMDMHDKGVTTGAVCVYPSRVADAVKTLKAANSSLPVASVATGFPAGQTPLETRLQEVRMAVDDGATEIDIVINRMLAITGQWEAMYDEICQFREACGDAHMKTILAIGELGTFTNVYKASLVSMMAGSDFIKTSTGKEAVNATYPVAIVMVRAIRDYFLSTGHKVGFKPAGGIRTAKESLVWLTLIKEELGNDWLCPHLFRLGASSLLADIERQVGPHTKKKHNKRESLIYHHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1B9</accession>
    <name>A0A672Z1B9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z1B9"/>
    <dbReference type="Ensembl" id="ENSSORT00005010992.1">
        <property type="protein sequence ID" value="ENSSORP00005010650.1"/>
        <property type="gene ID" value="ENSSORG00005005762.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1B9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="145" mass="16683" checksum="1FB4BBC6B678BEB1" modified="2020-06-17" version="1">PYLCEFSQHASAVVVQAYEGMESVLLPYQYSGMLPEVPSVVWRRSDLNQTVIHLLKDKSGDVKKEYRYSERTSMKTNALDSRDFSLTLKKPQLSDSSNYTCNLQWETEWKETKERRLIQVQLQVKGQTQTTPTAVDTGQILRTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1U2</accession>
    <name>A0A672Z1U2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115431308</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z1U2"/>
    <dbReference type="Ensembl" id="ENSSORT00005010895.1">
        <property type="protein sequence ID" value="ENSSORP00005010557.1"/>
        <property type="gene ID" value="ENSSORG00005005711.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1U2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF20">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="33"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="252" mass="29017" checksum="7019F4C589E0758C" modified="2020-06-17" version="1">MWTEVVCGAVVFPGLFHGFRRVLQSVFTHWTQADVVSVSERLVSAIHASLATAAGMTVVTSCRDVMTDSHWLVNGFVLFGAPYMANDIYAMYLSHYHTQRVKGHMSTYRRHSFQTMKEFLIREWMLVLHHVVLLLVFLPITLFFRRGLGDFFIGCFFITEFSTPFISIGKILIQVKLSDGKVHRINGIFVLLSFFTCRILLFPFMYWMYGHHAGISLHRVASIYPCTLLLKKANRLYLKQMGNSCKDRSKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z328</accession>
    <name>A0A672Z328_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z328"/>
    <dbReference type="Ensembl" id="ENSSORT00005011567.1">
        <property type="protein sequence ID" value="ENSSORP00005011190.1"/>
        <property type="gene ID" value="ENSSORG00005006013.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z328"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="2">
        <location>
            <begin position="70"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="161" mass="17698" checksum="DAB691D18CE2E4FF" modified="2020-06-17" version="1">MNPAELTQVQEAIQFQGHRLGGHERALHSLVDRMTQLATQVSQLTRTQAVAAAVSGESRAANPPVRDSAEPNIPAPSKYSGNPDTCRDFLTQIQLIFDSQPVRFAKDSVKIAYLASLLEGPPLSYFNALYEQGSPAVLSFSALLSELKRVYSWCKDCETRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z391</accession>
    <name>A0A672Z391_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mgat4 family member C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mgat4c</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993243.1">
        <property type="nucleotide sequence ID" value="XM_030137383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z391"/>
    <dbReference type="Ensembl" id="ENSSORT00005011587.1">
        <property type="protein sequence ID" value="ENSSORP00005011209.1"/>
        <property type="gene ID" value="ENSSORG00005006022.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421479"/>
    <dbReference type="CTD" id="25834"/>
    <dbReference type="InParanoid" id="A0A672Z391"/>
    <dbReference type="OrthoDB" id="2016523at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056576">
        <property type="entry name" value="MGAT4_A/B/C_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF14">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23524">
        <property type="entry name" value="MGAT4A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGAT4 conserved region" evidence="4">
        <location>
            <begin position="61"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 A/B/C C-terminal" evidence="5">
        <location>
            <begin position="337"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="55641" checksum="2530333EE16183DD" modified="2020-06-17" version="1">MRLLWKSLDKMRCLRKRSTMPFLGFLITFLLFLNLYIEDGYVLEEDKRQLRETSVHPASSERYVHTFRDLSNFSGTINVTYRYLAGTPLNRKKYLTIGLSSVKRKRGNYLLETIKSIFDQSSYEELKEIVVVVHLADFDLAWCENLVQEITRKFAHHIIAGRLLVIQAPEEYYPSLDGLKRNYNDPEDRVRFRSKQNVDYAFLLNFCTNLSHFYMMLEDDVRCSRNFLTALKKVITSREGSYWVMLEFSKLGYIGKLYHSKDLPRLAHFLLMFYQEMPCDWLLIHFRGLLAQKDVIRFKPSLFQHMGYYSSYKGAENKLKDDDFEEDSLDIPDNPPASLYTNINVFENYDATKAYSTVDEYFWGKPPSTGDFFVIVFNKSTKISKIKIATGSDDRQNDILHHGALEVGEKLIGTKKGKQCSSYITIGEFKNGNIEVQDVDHKIAFDIECVRIVVTASQKEWLIIRSISLWTTQPPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6R9</accession>
    <name>A0A672Z6R9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial elongation factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mief2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z6R9"/>
    <dbReference type="Ensembl" id="ENSSORT00005012921.1">
        <property type="protein sequence ID" value="ENSSORP00005012524.1"/>
        <property type="gene ID" value="ENSSORG00005006576.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z6R9"/>
    <dbReference type="OrthoDB" id="5964386at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090141">
        <property type="term" value="P:positive regulation of mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000003">
        <property type="entry name" value="Mitochondrial dynamics protein MID51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.90:FF:000002">
        <property type="entry name" value="Mitochondrial dynamics protein MID51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
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        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045909">
        <property type="entry name" value="MID49/MID51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049097">
        <property type="entry name" value="MID51-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16451:SF13">
        <property type="entry name" value="MITOCHONDRIAL DYNAMICS PROTEIN MID49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16451">
        <property type="entry name" value="MITOCHONDRIAL DYNAMICS PROTEINS 49/51 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
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    </dbReference>
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        <property type="entry name" value="Mab-21"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial dynamics protein MID51-like C-terminal" evidence="14">
        <location>
            <begin position="151"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="13">
        <location>
            <begin position="366"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041786"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00043160"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF21297"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="468" mass="51970" checksum="19C81DECA561C120" modified="2020-06-17" version="1">MNFHGSRRRGEDGIAMVIDFLLSNARLVLGVGGAAVLGIATLAVKRLIERAGRAADAEKVEQKMAESWEELSLVSASPTLLKKGIEGVVMKHVAKATKQQKGDLCQQPPTSSLELQPKPESKSKRLQLCVLSLQERLQQYYHTRAALAPQEVQRAQSLALDICTEIQGFLHSRHPDMPLGEMSLGGSLLDDLQVVSADHACLLVPLQLEASLWHLVPGEETLLTHPLHWMVRRVNLEYFPRGRSYWDRYLVGGYLSSEAVVNMFSKTVMETMNWPTISSTLDCLVRPVPGGPDLRLEIRPGNENGGIESSTPPLFISMLPLLRDEDVALTAQPELTSPWVNAWHLSLYPWETQRLAQLDASDGGYRRLTLKILKAVCRLNPALRPLKAAPLANLILHLSDGESDWSESSLDVRFQQCITELIGYLEQGALHSYFKPAVNLLSSLSEDQVDQMGFMLYCAVSEPEILLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7B5</accession>
    <name>A0A672Z7B5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Microfibril-associated glycoprotein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115426264</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z7B5"/>
    <dbReference type="Ensembl" id="ENSSORT00005012764.1">
        <property type="protein sequence ID" value="ENSSORP00005012367.1"/>
        <property type="gene ID" value="ENSSORG00005006514.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:extracellular matrix structural constituent"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:blood coagulation, common pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:fibrinolysis"/>
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    </dbReference>
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        <property type="term" value="P:platelet aggregation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="FIB_ANG-like"/>
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        <property type="entry name" value="Fibrinogen-like_C"/>
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    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
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        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
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        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF5">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Fibrinogen_C"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FIBRINOGEN_C_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="5">
        <location>
            <begin position="34"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="260" mass="29370" checksum="487EA275CFCFD49D" modified="2020-06-17" version="1">MHIITCFQCSYSYLAFIDIRATLLLALAALVHSDSQFYLPIDCDDIFRHDNRSSSGIYTIYPGGPTTPLKVYCDMNTDGGRWTVFLRRLDGSENFFRPWNNYKSGFGNVAGEYWLGLENIFLLSMRKKNEMRVDMEDWSGTTASANYDSFSIDSELSGYQLHLGSFTGGSAGDGLTSAKDMKFTTYDKDQDTWDYNCAQHFIGGFWYGSCHGANPTGMYAPNGALPFDNVQALWSTFKAPNYSLKRINMKIRSISKCYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAM7</accession>
    <name>A0A672ZAM7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ncoa5</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029989572.1">
        <property type="nucleotide sequence ID" value="XM_030133712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZAM7"/>
    <dbReference type="Ensembl" id="ENSSORT00005014804.1">
        <property type="protein sequence ID" value="ENSSORP00005014381.1"/>
        <property type="gene ID" value="ENSSORG00005007364.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419093"/>
    <dbReference type="CTD" id="57727"/>
    <dbReference type="InParanoid" id="A0A672ZAM7"/>
    <dbReference type="OrthoDB" id="10044938at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12341">
        <property type="entry name" value="RRM_hnRNPC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052600">
        <property type="entry name" value="Nuc_rcpt_coact/corep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295:SF3">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="443"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="619"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="641"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="651" mass="72971" checksum="884EE21A8B3B8B01" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCB2</accession>
    <name>A0A672ZCB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RILP-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rilp</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZCB2"/>
    <dbReference type="FunCoup" id="A0A672ZCB2">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014980.1">
        <property type="protein sequence ID" value="ENSSORP00005014555.1"/>
        <property type="gene ID" value="ENSSORG00005007441.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZCB2"/>
    <dbReference type="OrthoDB" id="10069524at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14445">
        <property type="entry name" value="RILP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1770:FF:000003">
        <property type="entry name" value="RILP-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051241">
        <property type="entry name" value="DZIP_RILPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021563">
        <property type="entry name" value="RILP_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502:SF7">
        <property type="entry name" value="RAB-INTERACTING LYSOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21502">
        <property type="entry name" value="ZINC FINGER PROTEIN DZIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11461">
        <property type="entry name" value="RILP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161256">
        <property type="entry name" value="RILP dimerisation region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51776">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51777">
        <property type="entry name" value="RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RH1" evidence="14">
        <location>
            <begin position="18"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="15">
        <location>
            <begin position="286"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="254"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="402"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="118"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="402"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="435" mass="50000" checksum="FBAA3C13A4F68636" modified="2020-06-17" version="1">MPLVSDMEEPEAHSQAKPTESCFDRTFAALTVDDVYEIAKLIGTEVEKLIDGFGKDSVVGLVPKIVKVLELLESFASRNHAHKLKEEELLKTFETIQLQQQKKRGGKDSEESNDRNEIRDLQQKEQQFRRRCEELQVQVQQLQEDKEELQSRLKGSHAKEDRVQRQEREVMLKLKEVVDRQRDELRAKVQEITTISKEVEALQEQLERFMKMNAELRHKQNVLQAQLKSTVERKADMEADLKEKSKEIENLQALLDRTNSNTPSSPSQSTQKSTKKSTGDQKDPDMPCFTKKEVRDIISERNELKTNLFLVQEELNYYQREILNEERCPGFLLEAVRSVIKKRRKLIKAKMLGIPANECSSDEEEKSPLFGQTEVDGTEVDDTDKPAESRIRNLFGFLTRSGSGRSPTHMSNSASTWEIIGDSEANEDTEQQSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZEK6</accession>
    <name>A0A672ZEK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115438364</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZEK6"/>
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    </dbReference>
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        <property type="gene ID" value="ENSSORG00005007579.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZEK6"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="240"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="102"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="385" mass="43508" checksum="37167545375B9554" modified="2020-06-17" version="1">MNGTTLPRFRPWGGPERRDNTVHQRWRRERRDDQRPEINSQQVVGVISHSELCLSLMETYEEAELCFPKLLNASCQKPVNSQVKNLILHILLFIFSPMTVALNLLVIITVSHFRQLHTPTNLILLSLAVSDLLVGLVPMPGEVYLHFNCWFLGDLMCSSYNLITYVSVSASVGNMVLISIDRFIAICDPLHYTHKVTITRVKVHVCLCWFGSVGFSILILNDNLTHPGKYHICYGQCFVIIGLIARIIDLVFSFFLPIVVIVVLYMSVFVVAVSQARAMRSHITAITAQQSVKGVANKSELKAARTLGVLVLVFLNCYCPYFGMLFTEDVALHNASFSYVTFLLHLNSCLNPLIYALLYPWFRKAIKLIFTLQILQPGSSETNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFE0</accession>
    <name>A0A672ZFE0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transmembrane protein 120A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tmem120aa</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM120 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZFE0"/>
    <dbReference type="FunCoup" id="A0A672ZFE0">
        <property type="interactions" value="613"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015708.1">
        <property type="protein sequence ID" value="ENSSORP00005015218.1"/>
        <property type="gene ID" value="ENSSORG00005007698.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZFE0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012926">
        <property type="entry name" value="TMEM120A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433:SF1">
        <property type="entry name" value="ION CHANNEL TACAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07851">
        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="346" mass="41076" checksum="1A92B9A89630B1F6" modified="2020-06-17" version="1">MLSSPTGFAECLHEWEDLEKDYQQIQETHRLYKQKLEEVTRLQDSCSSAIARQRKKQRELTASLLECKEKHSTSKLSVEDMEAIDEIQNSINDRSSAFFEMEAFLPKKNGLYLSLVLGNVNVTLLNKQAKFAYKDEYEKFKLVLTVILFLFSFMCRLISYRALDALFNFLLVWYYCTLTIRESILISNGSRIKGWWVFHHYVSTFLSGVMLTWPEGVLYQMFRSQFLTYSLYQSFVQFLQYYYQSGCLYRLRALGERHNMDLTVEGFQSWMWRGLSFLLPFLFFGQFWQLYNSITLFRMFQLPECKEWQVAMCGCSYMVLFMGNFFTTVGVVYQKYMNNQDKSKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFW8</accession>
    <name>A0A672ZFW8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZFW8"/>
    <dbReference type="Ensembl" id="ENSSORT00005015843.1">
        <property type="protein sequence ID" value="ENSSORP00005015352.1"/>
        <property type="gene ID" value="ENSSORG00005007793.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZFW8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001235">
        <property type="entry name" value="Si:ch211-119o8.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001370">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000264">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000443">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF158">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="340"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="381"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="382"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="567" mass="65728" checksum="A88C4D54FC0925B8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGY2</accession>
    <name>A0A672ZGY2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor RBM22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">RNA-binding motif protein 22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">rbm22</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLT11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030001835.1">
        <property type="nucleotide sequence ID" value="XM_030145975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZGY2"/>
    <dbReference type="FunCoup" id="A0A672ZGY2">
        <property type="interactions" value="2198"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016543.1">
        <property type="protein sequence ID" value="ENSSORP00005016044.1"/>
        <property type="gene ID" value="ENSSORG00005008100.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427424"/>
    <dbReference type="CTD" id="55696"/>
    <dbReference type="InParanoid" id="A0A672ZGY2"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12224">
        <property type="entry name" value="RRM_RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000137">
        <property type="entry name" value="pre-mRNA-splicing factor RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000006">
        <property type="entry name" value="Putative pre-mrna-splicing factor rbm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057674">
        <property type="entry name" value="Znf-CCCH_RBM22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25584">
        <property type="entry name" value="zf-CCCH_RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="10 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="232"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="16">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="389"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="427" mass="47617" checksum="9FD54273E9C9AB21" modified="2020-06-17" version="1">MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGTRMRFKKTEVCQTCSKMKNVCQTCLLDLEYGLPIQVRDTGLSVKDDVPKSDVNKEYYTQNMEREIANSDGTRPVGQLGKATSSSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYIGGLGDTVTDGDLKSHFYQFGEIRTITIVQRQQCAFIQFATRQAAEMAAEKSFNKLIINGRRLTVKWGRSQAARGKEGVKDGVSEMGTRLDPVPGLPGALPPPPALDEEAPANYFNLDPSTSPAVMNIALPPPPGINPPPPGFGPPMFHPMGPMAPPMPPPMSMRPPGQIHYPSQDPQRMGAHAAHGSRHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHA0</accession>
    <name>A0A672ZHA0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Organic solute carrier partner 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">oscp1b</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZHA0"/>
    <dbReference type="FunCoup" id="A0A672ZHA0">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017151.1">
        <property type="protein sequence ID" value="ENSSORP00005016641.1"/>
        <property type="gene ID" value="ENSSORG00005008420.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZHA0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019332">
        <property type="entry name" value="OSCP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439">
        <property type="entry name" value="OXIDORED-NITRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439:SF0">
        <property type="entry name" value="PROTEIN OSCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10188">
        <property type="entry name" value="Oscp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42893" checksum="AE78FC4610E4A6B3" modified="2020-06-17" version="1">MSSRTLPLLFINLGGEMLYILDQRLRAQNIPADKAKKVMNDIITTMFNKKFLEELFKPQELYSKKALRTVFDRLAHASIMRLNQASMDKLYDLMTMAFKYQVLLCTRPKDILLVSFNHMDAIKDFVKDSPSILSQVDETYRQLIEMYTPLSSGEFQLIRQTLLIFFQDMHIRVSIFLKDKVQNSNGRFVLPTSGPVPYGTQIPGLIRMFSCTGEEVSRLQFNNGGNYTSPLREGSFEIFGDRVTKLGTNMYSVSRPVETHMSGTSKNSAQHTKVSVSAVPNPLAKEELNLLARLMGGLEVQKPGNADNGFRVNLFATDEEEEEALISRPDELSYGVINIQATKDQHANAELAKIMGEFTESGDQSPSASSKGDDLLAMMDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJP1</accession>
    <name>A0A672ZJP1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein axonemal assembly factor 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dnaaf4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Dynein axonemal particle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZJP1"/>
    <dbReference type="Ensembl" id="ENSSORT00005017207.1">
        <property type="protein sequence ID" value="ENSSORP00005016697.1"/>
        <property type="gene ID" value="ENSSORG00005008433.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJP1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030331">
        <property type="term" value="F:nuclear estrogen receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003351">
        <property type="term" value="P:epithelial cilium movement involved in extracellular fluid movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06469">
        <property type="entry name" value="p23_DYX1C1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000176">
        <property type="entry name" value="dynein assembly factor 4, axonemal isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000015">
        <property type="entry name" value="dynein assembly factor 4, axonemal isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037894">
        <property type="entry name" value="CS_DYX1C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052004">
        <property type="entry name" value="Dynein_assembly_factor_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46492">
        <property type="entry name" value="DYNEIN ASSEMBLY FACTOR 4, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46492:SF1">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="CS" evidence="13">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="372"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="106"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="135"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="439" mass="50385" checksum="45F606CEF096EEEB" modified="2020-06-17" version="1">MPVLVPDYTWTQTQSTVHIHLPLKGAKPGRVHVTSTDEYLKVHFQPFLLEVFLFQPVDDDRSTARIGNGVANITLYKKTNNQWEQLMINTTDKEVMKQIRMKALMKNQEKLTAESKTKTQKRQEERKYTLQSMMKLEDEERDSIRKIKDTERKKTTAELEEWQTKQKGEEDARVQRKEKERSHGGKNKPGNGSNPLTERPSSVYSMCLGFGSNNPFFCLDRRSVSEVKKPEQKHLPAPRAAGNIQVKFTPRVFPTALRESRVAEEEEWLRKQAEARRSVNAEVEELKDLKDEERNPDWLKEKGDRCFLTGDYLGAVNAYNLAIRLNRKIPALYSNRAACHLKLKNLHKAIEDCSQALSLLTPPVQSNAASRVRVHVRRGTAFCHLQLYTEGLLDYQAALNIDPENQALQADAQRIRNVIQGLEPETQQNTTKGCESPNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLW0</accession>
    <name>A0A672ZLW0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heme-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">soul4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZLW0"/>
    <dbReference type="Ensembl" id="ENSSORT00005017931.1">
        <property type="protein sequence ID" value="ENSSORP00005017413.1"/>
        <property type="gene ID" value="ENSSORG00005008706.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLW0"/>
    <dbReference type="OrthoDB" id="9944327at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF24">
        <property type="entry name" value="HEME-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="248" mass="28750" checksum="D05736AE06C2537E" modified="2020-06-17" version="1">MALISLEDLDGLEDEQLDDDITDNPEPMDEEDRDRMLTHWRNVASTHHVTVPSEMTGPIQEMTRNSHQREPLPFAPIARHEKMGEVQYEERVYPAGFWACVTRSEDLYEQSISMGFMKLMRFICKENSAGRYLGMTIPVVSTIHMMQDGNMFDKEVSTAFFLPSEHQTNPPQPGDSDISIVHREPIRVIARTFFGTTTEETVSHQIALLWEILGVTDDVRTDNYMVAVYENPSVQRRRNEIWFLRRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZM59</accession>
    <name>A0A672ZM59_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein ILRUN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ilrun</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZM59"/>
    <dbReference type="FunCoup" id="A0A672ZM59">
        <property type="interactions" value="1678"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018051.1">
        <property type="protein sequence ID" value="ENSSORP00005017532.1"/>
        <property type="gene ID" value="ENSSORG00005008757.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZM59"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14349">
        <property type="entry name" value="UBA_CF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000015">
        <property type="entry name" value="Chromosome 6 C6orf106 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000289">
        <property type="entry name" value="Chromosome 6 open reading frame 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039517">
        <property type="entry name" value="C6orf106_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nbr1 FW" evidence="9">
        <location>
            <begin position="82"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="292" mass="32229" checksum="BD742D4D77156751" modified="2020-06-17" version="1">MEGTDMDVDAELMQKFSCMGTTDKDVLISEFQRLLGFQLNPAGCAFFLDMTNWNLQAAIGAYYDFESPNVNTPSMSFVEDVTIGEGESVPPDTPFTKTWRIQNTGAESWPPGVCLKYIGGDQFGHVNAVMVKSLDPQEISDVSVQMRSPTNPGMYQGQWRMCTATGLFYGDVIWVILSVEVGGLLGVTQQLSSFETEFNTQPQRNVQGDFNPFASPQKNKHDATDDSFRDPGGAWERTQEQIQQDQNGLSHNAVNRASNGLQTNLSVVTYGQSCRRKHVTDGPLMESKPLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZNW3</accession>
    <name>A0A672ZNW3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nuclear factor interleukin-3-regulated protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115426160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000019.1">
        <property type="nucleotide sequence ID" value="XM_030144159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZNW3"/>
    <dbReference type="Ensembl" id="ENSSORT00005019466.1">
        <property type="protein sequence ID" value="ENSSORP00005018915.1"/>
        <property type="gene ID" value="ENSSORG00005009307.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426160"/>
    <dbReference type="InParanoid" id="A0A672ZNW3"/>
    <dbReference type="OrthoDB" id="6151507at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14694">
        <property type="entry name" value="bZIP_NFIL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047229">
        <property type="entry name" value="NFIL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047106">
        <property type="entry name" value="NFIL3-like_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284:SF6">
        <property type="entry name" value="HYPOTHETICAL LOC799271-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284">
        <property type="entry name" value="NUCLEAR FACTOR INTERLEUKIN-3-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="98"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="410" mass="44882" checksum="8D835230F4BEB80E" modified="2020-06-17" version="1">MTGQTAGGVTQDPAVPLMLNVEGLESRPLGSATTFSDEAITILASTSQLARTLLGRTFALSRSESVSSAEAKAGSSCDEDSTNSVRRKREFTPNDKKDEGYWDKRRKNNEAAKRSREKRRANDLVLERRVLGLLEENARLRAELLALKFRFGLVKDPSEVTILPLSTALCTNTTPNNTPYYHQHTDNPSQTTTAQGTSTHHIQPPPQGSSYTLRGAGPPSNHSVSEESGVLCNSNIDSPVFFDETLGGPSLRNQVEDPQGCHAGPLEVNEGPYMTRLDSPEGLRSLPHKLRFKAPSSDGGEVSPTYDSRCGGPPVATVGPNIQMRQHQQTGSDSRTENQPAWTREEACCGLRQQCHTRPFGYYNSSSQQNSRDIKHSTEDLGLRSQISCLSEEVAQLKKLFSQQLLSKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZPA3</accession>
    <name>A0A672ZPA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Histone acetyltransferase</fullName>
            <ecNumber evidence="25">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">kat7b</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 25">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 25">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="25"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZPA3"/>
    <dbReference type="FunCoup" id="A0A672ZPA3">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019646.1">
        <property type="protein sequence ID" value="ENSSORP00005019090.1"/>
        <property type="gene ID" value="ENSSORG00005008797.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZPA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036409">
        <property type="term" value="C:histone H3-K14 acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036408">
        <property type="term" value="F:histone H3K14 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902035">
        <property type="term" value="P:positive regulation of hematopoietic stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045935">
        <property type="term" value="P:positive regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000092">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
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        <property type="entry name" value="MYST_HAT"/>
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    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
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        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
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        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
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        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="20 25" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 24" id="KW-0863">Zinc-finger</keyword>
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            <end position="133"/>
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    </feature>
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        <location>
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            <end position="232"/>
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            <position position="479"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
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        <source>
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    </evidence>
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        </source>
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            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="ARBA" id="ARBA00023315"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51726"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019090.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="582" mass="67202" checksum="1FAA9CFD78310AB9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQN8</accession>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
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            <taxon>Neoteleostei</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="637" mass="75168" checksum="E242CDD3A9C3D839" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUZ2</accession>
    <name>A0A672ZUZ2_9TELE</name>
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        <recommendedName>
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        <lineage>
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            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="PANTHER" id="PTHR21590">
        <property type="entry name" value="SEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21590:SF3">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12877">
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        <property type="match status" value="1"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16664" checksum="D6D76A75982FB69C" modified="2020-06-17" version="1">MPVYHPQCSVHKITNWVSSVCLLLRFVGPTDNIYSCSFVQMLEQRLENAFDEAQDKVLETYNRLTVEIQSVSQDPGSPSVTLVYVVKNQDAILNGTISSGLLSQLTAELVGYFLFYPPLIIAERKLNKKPHCASLRRVCSVGENYIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZC8</accession>
    <name>A0A672ZZC8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor), tandem duplicate 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">itga2.2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZZC8"/>
    <dbReference type="FunCoup" id="A0A672ZZC8">
        <property type="interactions" value="606"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022490.1">
        <property type="protein sequence ID" value="ENSSORP00005021842.1"/>
        <property type="gene ID" value="ENSSORG00005010632.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZC8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF23">
        <property type="entry name" value="INTEGRIN ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1054"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="25"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="161"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="455"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="520"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="582"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1101" mass="120753" checksum="93B5224EDEB7C105" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0S4</accession>
    <name>A0A673A0S4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc transporter ZIP9</fullName>
            <shortName evidence="7">ZIP-9</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Solute carrier family 39 member 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Zrt- and Irt-like protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115434755</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Transports zinc ions across cell and organelle membranes into the cytoplasm and regulates intracellular zinc homeostasis. Participates in the zinc ions efflux out of the secretory compartments. Also functions as membrane androgen receptor that mediates, through a G protein, the non-classical androgen signaling pathway, characterized by the activation of MAPK3/MAPK1 (Erk1/2) and transcription factors CREB1 or ATF1. Moreover, has dual functions as membrane-bound androgen receptor and as an androgen-dependent zinc transporter both of which are mediated through an inhibitory G protein (Gi) that mediates both MAP kinase and zinc signaling leading to the androgen-dependent apoptotic process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A0S4"/>
    <dbReference type="Ensembl" id="ENSSORT00005023496.1">
        <property type="protein sequence ID" value="ENSSORP00005022829.1"/>
        <property type="gene ID" value="ENSSORG00005011040.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A0S4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045891">
        <property type="entry name" value="ZIP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39 ZINC TRANSPORTER , MEMBER 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16133:SF4">
        <property type="entry name" value="ZINC TRANSPORTER ZIP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="321" mass="34050" checksum="BE614259E3E1E533" modified="2020-06-17" version="1">MDGGLTITLISVAMFIGCFLLGFIPLLFRLSETSMKYITILGAGLLCGTALSIIIPEGVGLIVESLTAGFVLHLLSADSAPVPTSLNNSETNVTSINSGPRPQFLIGVALTFGFILMFIVDQIGGYLSTRGKSSGFLWVYLHHISLSITHLLLYCLISGRIARSPISIGITATLGLVIHAAADGFALGAAVATSEVTVQVIVFLAVILHKAPAAFGLVSFLMHSGLEKKYIQGHLLAFSAAAPIVAIITYFILHASGSLFQHSSTGMGMLFSAGTFLYVATVHVLPEIRYPHQHRYLGLLETMTLIFGVALTVVDVGHSHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1P6</accession>
    <name>A0A673A1P6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Charged multivesicular body protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Chromatin-modifying protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">chmp7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029999075.1">
        <property type="nucleotide sequence ID" value="XM_030143215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A1P6"/>
    <dbReference type="FunCoup" id="A0A673A1P6">
        <property type="interactions" value="1387"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023317.1">
        <property type="protein sequence ID" value="ENSSORP00005022652.1"/>
        <property type="gene ID" value="ENSSORG00005011015.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425585"/>
    <dbReference type="CTD" id="91782"/>
    <dbReference type="InParanoid" id="A0A673A1P6"/>
    <dbReference type="OrthoDB" id="10250120at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057471">
        <property type="entry name" value="CHMP7_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF21">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25239">
        <property type="entry name" value="WHD_CHMP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25880">
        <property type="entry name" value="WHD_CHMP7_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CHMP7 winged helix" evidence="12">
        <location>
            <begin position="144"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="456" mass="51407" checksum="FDF15AE4F3366C6D" modified="2020-06-17" version="1">MSNSTKMTPPPEWDDDERMNFLFSDFKENRDVNTTDWDSKMDFWSGLFLQTCRDRGSVCVNLQDLNKTFRRKEKSPLGLGTVIQSMARCGKIQRESEFAANVDCGWLSWGVGLLLVKPLKWTFSTLLGSSRVPLGESFVVIELVKEKAAQLLEAYRSSEFASRSILSFQELRDLSSGVCVDESTMCMALLQLQRDKQVMVSLHEGEKIVKFCQTGQDRVSPVNDVDIGIYQLQRSEKLLGERVEKLSLEADKCKEEARVLLREGKKSQALRCLRGRKRVEKRADNLFAKLESIRGILDRIAQSQTDKMVMQAYQAGVAALRLSLKDVTVERAESLVDQIQELCDTQDEVNQTLSSGVTSADEDMDELEEELKSLLEETQTDISSGLPEVPSKPLRPSGEYSLMGNDLLSSLPAVPQRNMNFATEQLEQELNQLTLTDSGFQHKKITSPAKRLEPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2H4</accession>
    <name>A0A673A2H4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">xpot</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <text evidence="10">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A2H4"/>
    <dbReference type="FunCoup" id="A0A673A2H4">
        <property type="interactions" value="1673"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024398.1">
        <property type="protein sequence ID" value="ENSSORP00005023700.1"/>
        <property type="gene ID" value="ENSSORG00005011325.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A2H4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000105">
        <property type="entry name" value="Exportin for tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="11">
        <location>
            <begin position="108"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="12">
        <location>
            <begin position="327"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="979" mass="111531" checksum="0B40D455AEE2D610" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5J8</accession>
    <name>A0A673A5J8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein SSUH2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ssuh2rs1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="391" mass="42977" checksum="4375A05FAE80E998" modified="2020-06-17" version="1">MESQPIMSHNRTYGVANPGYVPEAVAGAPAMFAPPAAGSFGPSAPPANMFDTVPGYEGTMAGEGGGFLPPPMPLNPVPQPQPGPEQPQWNIPSITEDTAKEAFALFASSKCCYSSAPAKEGGITNMEAFNTYRYRLETFTESRSTQWAQEPYHGQPVDAYSSPPPGPWDIPAKVPSFFQDSKQIIKVPYTSSMKTCHVCLGMGRKPCRDCAGAGNKACWVCNGSGYRHGDDRCHHCNGRGRENCSHCHGQGSKQCPTCHGKQQLLVYINLTVKWTNNSDNYVVEQSSGLQVENLSKVSGQELFRDSQYLVYPLMSFPDMAVVNASQRFVSEHQGKFSQTSRILQQRQTIELIPVTRVTYSWKGKSHIYFVYGNEFKVSTDDYPATCCCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A7F8</accession>
    <name>A0A673A7F8_9TELE</name>
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        <submittedName>
            <fullName evidence="6">Calcium release activated channel regulator 2B</fullName>
        </submittedName>
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        <name evidence="6" type="primary">cracr2b</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
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        <location>
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        <location>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="665" mass="75717" checksum="4EE362D41A359C28" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A7T2</accession>
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        <submittedName>
            <fullName evidence="2">snRNA-activating protein complex subunit 1-like</fullName>
        </submittedName>
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        <name evidence="2" type="primary">LOC115426799</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <sequence length="350" mass="40604" checksum="21B2B0FACD11DABA" modified="2020-06-17" version="1">MPRCPPTYAEFFFDPLTEDVEELLGRFQHRDSVRYEVFSSIWREMGFSDVFFGIPSMNEMKRFCRVTLATAVKYFLPPYSFQIRVGGLYLMFGFYHTQLAIPPLKIRLALKDWSDVQKFLKESVASGHYDVVYIYQKLVFSKAFHYTAMPHLLSFQKQRKKKKNEVCAGFLGRTTAIQELLSAEIFDEMANIQNHYEKMKTTSEEVKAKQISMTHQDFVTGLKDCMSAFISWQQKTFSQDDNKDKNSDEDEDEEKAAGAQCSSRARLLSSIKQKSYSQFQEASRSRRHRQPEAMEDASSGPEQVQKAANMQSRKPSLRARTHKNLRVTQDKKRINNWLLTAPELGGSVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAV2</accession>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate</text>
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            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35973"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:24565"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>acetoacetate + succinyl-CoA = acetoacetyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:25480"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
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            <dbReference type="Rhea" id="RHEA:25481"/>
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    </comment>
    <comment type="pathway">
        <text evidence="2 9">Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the 3-oxoacid CoA-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.5" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673AAV2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <location>
            <position position="369"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051996"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026386.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="584" mass="63624" checksum="1D87E7DB02903EBF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADW9</accession>
    <name>A0A673ADW9_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Si:dkey-19b23.7</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <location>
            <begin position="66"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25377"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="209" mass="24071" checksum="CEE3D110BD839E37" modified="2020-06-17" version="1">FRRFLRVESLSCFHLQGFKYFQPWLRGKEELLLTVVNEDLVCVNWTVLDSLTGTVTDTEDHLLPASPSEREIAVPEVNCTLFLLAGYVKYGRPYAWIRSNHERLVNIGGTDSLVKDTPMKLKSISDWQTRGIRVWDVVSELVCLCTVPSPSNPFALDMRYVKSLPLPDRFLVTGALLNFLETYVVYGNRDEMHYDKGTLGHRWNQTKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AED2</accession>
    <name>A0A673AED2_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">RNA binding protein fox-1 homolog</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="primary">LOC115423903</name>
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    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">RNA-binding protein that regulates alternative splicing events.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AED2"/>
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        <property type="protein sequence ID" value="ENSSORP00005027636.1"/>
        <property type="gene ID" value="ENSSORG00005013196.1"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
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    </dbReference>
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        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RBFOX1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF25">
        <property type="entry name" value="RNA BINDING PROTEIN FOX-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12414">
        <property type="entry name" value="Fox-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ataxin_2_bd_A2BP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="161"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="113"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="362" mass="39281" checksum="9C05972B6829D7AA" modified="2020-06-17" version="1">ATAVTWTRSNPETGNTLCKSGSIAVFGNVVYSGLLNDHLWHLGVPLFTRLGNQEATNPPEAMAQPYTPAQYPPPPQNGIPAEFAAPHPLPTQDYTGQSRVPEHAMTLYTPTQTHNEQAGTDNSTPAITATTTAPTDDVTQTEGSQQLQLQHSDSSEKQQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFETSTDADRAREKLNGTIVEGRKIEVNNATARVMTNKKVANPYTNGWKLNPVVGAVYGPELYAVTGFPYPATGATVAYRGAHLRGRGRAVYNTFRTAPPPPPIPAYGGGYAAYRFAQPTTTAAYSDSYGRVYATADPYHHTIGPAATYSVGTMVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEZ2</accession>
    <name>A0A673AEZ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcription elongation factor A (SII), 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFS-II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AEZ2"/>
    <dbReference type="Ensembl" id="ENSSORT00005028618.1">
        <property type="protein sequence ID" value="ENSSORP00005027821.1"/>
        <property type="gene ID" value="ENSSORG00005013314.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AEZ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000001">
        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF3">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR A PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS central" evidence="10">
        <location>
            <begin position="11"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="9">
        <location>
            <begin position="124"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="171" mass="19417" checksum="339724572F6FC1EE" modified="2020-06-17" version="1">GPSHSDPVPPVRNKCRELLVAALQTDDDHKTIGVDCEHLAIYQEFKSTDMKYKARLRSRISNLKDQKNPDLRRNVLCGNISAQRIACMTAEEMASAELKQIREALTKESIREHQLSKVGGTETDMFICNKCRGKNCTYTQVQTRSADEPMTTFVVCNSCGNRWKVRNNQHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHT4</accession>
    <name>A0A673AHT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma</fullName>
            <ecNumber evidence="8">3.1.4.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115424930</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 8">Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the rod/cone cGMP-PDE gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.35" evidence="8"/>
    <dbReference type="RefSeq" id="XP_029998190.1">
        <property type="nucleotide sequence ID" value="XM_030142330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AHT4"/>
    <dbReference type="FunCoup" id="A0A673AHT4">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029031.1">
        <property type="protein sequence ID" value="ENSSORP00005028228.1"/>
        <property type="gene ID" value="ENSSORG00005013497.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424930"/>
    <dbReference type="InParanoid" id="A0A673AHT4"/>
    <dbReference type="OrthoDB" id="8525078at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042622">
        <property type="term" value="C:photoreceptor outer segment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045742">
        <property type="term" value="P:positive regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045745">
        <property type="term" value="P:positive regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1120.10">
        <property type="entry name" value="Retinal cGMP phosphodiesterase, gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006952">
        <property type="entry name" value="PDE6_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037030">
        <property type="entry name" value="PDE6_gamma_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122">
        <property type="entry name" value="RETINAL CONE RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE GAMMA-SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122:SF10">
        <property type="entry name" value="RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04868">
        <property type="entry name" value="PDE6_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000969">
        <property type="entry name" value="35-cGMP_Pdiase_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0140">cGMP</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6" id="KW-0844">Vision</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="91" mass="10065" checksum="5DDAB7808F6A355F" modified="2020-06-17" version="1">MNIDVPKPEAKTGGNKFPTRPTCPGSPNKGPPKFKQRNSRQFKSKPPKRGVIGFGEEIPGMEGLGTDFNVICPWEAYSHLELHELAQYGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKH0</accession>
    <name>A0A673AKH0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Histone H4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 14">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AKH0"/>
    <dbReference type="Ensembl" id="ENSSORT00005030585.1">
        <property type="protein sequence ID" value="ENSSORP00005029749.1"/>
        <property type="gene ID" value="ENSSORG00005014216.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AKH0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000012">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0164">Citrullination</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 14" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="15">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="92" mass="10343" checksum="B764222EF7D46F6C" modified="2020-06-17" version="1">CSGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMV8</accession>
    <name>A0A673AMV8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Si:ch211-113a14.22</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AMV8"/>
    <dbReference type="Ensembl" id="ENSSORT00005030588.1">
        <property type="protein sequence ID" value="ENSSORP00005029752.1"/>
        <property type="gene ID" value="ENSSORG00005014219.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AMV8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000091">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="149"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="14">
        <location>
            <begin position="200"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="236" mass="25670" checksum="3CD95CFF863244F5" modified="2020-06-17" version="1">TIERKVKGGNVNILKTTMPEPAKSAPKKGSKKAVTKTAGKGGKKRRKSRKESYAIYVYKVMKQVHPDTGISSKAMGIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSKLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTEKPGKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AP32</accession>
    <name>A0A673AP32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SAP domain-containing ribonucleoprotein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Nuclear protein Hcc-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">sarnp</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis. Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. The TREX complex is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway. Associates with DDX39B, which facilitates RNA binding of DDX39B and likely plays a role in mRNA export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with DDX39A. Interacts with FUS. Interacts (via the C-terminal domain) with DDX39B; the interaction is direct and facilitates RNA binding of DDX39B. Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex; TREX seems to have dynamic structure involving ATP-dependent remodeling; in the complex interacts directly with DDX39B in a ATP-dependent manner which bridges it to ALYREF/THOC4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the SAP domain-containing ribonucleoprotein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029995615.1">
        <property type="nucleotide sequence ID" value="XM_030139755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AP32"/>
    <dbReference type="Ensembl" id="ENSSORT00005032313.1">
        <property type="protein sequence ID" value="ENSSORP00005031435.1"/>
        <property type="gene ID" value="ENSSORG00005014953.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423052"/>
    <dbReference type="CTD" id="84324"/>
    <dbReference type="InParanoid" id="A0A673AP32"/>
    <dbReference type="OrthoDB" id="5837849at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000013">
        <property type="entry name" value="SAP domain-containing ribonucleoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052240">
        <property type="entry name" value="SAP_domain_ribonucleoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551:SF1">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAP" evidence="17">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="210" mass="23065" checksum="4432342202F68C1B" modified="2020-06-17" version="1">MAEVIELQKLKLAELRQECEARGLDTKGNKGELIARLQAYLEEHEEDVDVDDVLAEDAEDFAKVESANNVKVNQESKTPTTEEPPKTVVKINPPSSASERLQKRAERFNLPASAESKKAVRAARFGMPADASSPSPGVVVNSKAAVNVEQLKKRAERFGVSVSSISQKLEEDEKLKKRKERFGILTSSASGGGDDIEAKKMKRAERFGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APB8</accession>
    <name>A0A673APB8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673APB8"/>
    <dbReference type="Ensembl" id="ENSSORT00005031267.1">
        <property type="protein sequence ID" value="ENSSORP00005030413.1"/>
        <property type="gene ID" value="ENSSORG00005014502.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APB8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021684">
        <property type="entry name" value="WBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051994">
        <property type="entry name" value="WW_domain-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209:SF5">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11669">
        <property type="entry name" value="WBP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="276" mass="30649" checksum="B49112B72D82147C" modified="2020-06-17" version="1">GHCCGETGCCTYYYELWWFWLLWTVLILFSCFCAYRHRRAKLRIQQQQRQREINLIAYNGACNYPSSMLDLSFLASFKLPSYEEVAAQPSTPPPPYSPGPSALTSSQSSDNYTSCSCESCSLTSPSSTSFSVQVTDETYDSSRMSTPSEGPGSGPKSIPSPPKQALFSSNVAVFMHCNKEEEEEEEDDDEEDHFRHRRLTGDSGIEVCRCHVKREEQEQGDLQKEPLSKGATKECDSVASSTIQSPLRDDCAEHNATISSSSAIIPKTGEAIITVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APR6</accession>
    <name>A0A673APR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tnfrsf13b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673APR6"/>
    <dbReference type="Ensembl" id="ENSSORT00005031248.1">
        <property type="protein sequence ID" value="ENSSORP00005030397.1"/>
        <property type="gene ID" value="ENSSORG00005014492.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APR6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001782">
        <property type="term" value="P:B cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030889">
        <property type="term" value="P:negative regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1290.10">
        <property type="entry name" value="Tumor necrosis factor receptor superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015384">
        <property type="entry name" value="TACI_Cys-rich-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022317">
        <property type="entry name" value="TNFR_13B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15511">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15511:SF2">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09305">
        <property type="entry name" value="TACI-CRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="258" mass="27756" checksum="B30B480386E32AB9" modified="2020-06-17" version="1">MGKSCPAGLYWDTLVKSCVDCRMVCQHPHVVSRCISHCVSADCKALPGFYYDPLLKTCMSCAEVCGRPPEQCTGYCPKCFPCLPSAQPLPVTTKTLLVKASPQTPGPMGLVGLTVLDSALPLYLLLALCTVLLLSSLFLALGVFVRRTTSSKSSKAEPEVDTSNTEVVVQLGQGVNQPGRSTKGDSSRPPEHEPLDDSSPTETCVCVHCFPDLKALSQGHDPTLRTPLPINHQARTGNTLARTTSTNSQQLLCHYNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AR00</accession>
    <name>A0A673AR00_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase CBL</fullName>
            <ecNumber evidence="13">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">cblb</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="13"/>
    <dbReference type="RefSeq" id="XP_030010042.1">
        <property type="nucleotide sequence ID" value="XM_030154182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AR00"/>
    <dbReference type="FunCoup" id="A0A673AR00">
        <property type="interactions" value="1269"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032612.1">
        <property type="protein sequence ID" value="ENSSORP00005031729.1"/>
        <property type="gene ID" value="ENSSORG00005015096.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433010"/>
    <dbReference type="CTD" id="868"/>
    <dbReference type="InParanoid" id="A0A673AR00"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16709">
        <property type="entry name" value="RING-HC_Cbl-b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09920">
        <property type="entry name" value="SH2_Cbl-b_TKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14392">
        <property type="entry name" value="UBA_Cbl-b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.20:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000154">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024162">
        <property type="entry name" value="Adaptor_Cbl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014741">
        <property type="entry name" value="Adaptor_Cbl_EF_hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003153">
        <property type="entry name" value="Adaptor_Cbl_N_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014742">
        <property type="entry name" value="Adaptor_Cbl_SH2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039520">
        <property type="entry name" value="CBL-B_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024159">
        <property type="entry name" value="Cbl_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007">
        <property type="entry name" value="CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CBL-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02262">
        <property type="entry name" value="Cbl_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02761">
        <property type="entry name" value="Cbl_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02762">
        <property type="entry name" value="Cbl_N3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47668">
        <property type="entry name" value="N-terminal domain of cbl (N-cbl)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51506">
        <property type="entry name" value="CBL_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cbl-PTB" evidence="17">
        <location>
            <begin position="35"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="373"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="15">
        <location>
            <begin position="925"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="469"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="738"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="778"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="476"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="546"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="575"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="682"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="809"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="828"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="978" mass="108878" checksum="4952AF4D4CD4A052" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARP1</accession>
    <name>A0A673ARP1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Gamma-crystallin S-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">crygs2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ARP1"/>
    <dbReference type="FunCoup" id="A0A673ARP1">
        <property type="interactions" value="1087"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032864.1">
        <property type="protein sequence ID" value="ENSSORP00005031979.1"/>
        <property type="gene ID" value="ENSSORG00005015189.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARP1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF6">
        <property type="entry name" value="GAMMA-CRYSTALLIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="41"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="131"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="174" mass="20885" checksum="EFFC168A972D7AAF" modified="2020-06-17" version="1">MGRIVFYEDKNFQGRRYECDSDCSDFHTYLSRCNSIRVESGAWVVYERPNYMGYQYVLTRGEYSDYQRWMGLNDRISSCKIIHFTSGTQYKMQLYEKADFGGQVFEATEDCPSLLEKVRWREVNSCKVFDGWWVFYEHPDYHGRQYFLEKGEYRKPGDWGAISPAIQSFRRFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASD1</accession>
    <name>A0A673ASD1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC115412211</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">zgc:136439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ASD1"/>
    <dbReference type="Ensembl" id="ENSSORT00005033361.1">
        <property type="protein sequence ID" value="ENSSORP00005032471.1"/>
        <property type="gene ID" value="ENSSORG00005015414.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASD1"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE THYMIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42883:SF2">
        <property type="entry name" value="THYMIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="274" mass="30951" checksum="BC6C6728672A8787" modified="2020-06-17" version="1">MKAVILAAGYGTRLQRDVEADSSGQFAHLSGIAKPLLPVGHRALISHWVHALTASGCVDCIYVVTNALYCPAFEEWAADFINVKILNDHTKSNNGRLGAVTCLQLAVKHFNIEDHIMVIGGDTLFKEDFSLTQVQQRFYEVQAVDEDSCLLLSYQCKDDETSKYGILEVDSNLRVLCMKEKPHPSETKSRRACPCFYMLSKRCLSLLDAFLQEKKDASIEEKDAPGNFVSWLIHRKPVYVHQISGRFDVGNLPSYTECDLYFREKLQDVESYMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATA8</accession>
    <name>A0A673ATA8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Intraflagellar transport protein 172 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ift172</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IFT172 family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A673ATA8">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032427.1">
        <property type="protein sequence ID" value="ENSSORP00005031547.1"/>
        <property type="gene ID" value="ENSSORG00005014916.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATA8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000008">
        <property type="entry name" value="Intraflagellar transport protein 172 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000012">
        <property type="entry name" value="intraflagellar transport protein 172 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000013">
        <property type="entry name" value="intraflagellar transport protein 172 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001535">
        <property type="entry name" value="Intraflagellar transport protein 172 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002910">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056168">
        <property type="entry name" value="TPR_IF140/IFT172/WDR19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056157">
        <property type="entry name" value="TPR_IFT80_172_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722">
        <property type="entry name" value="IFT140/172-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15722:SF2">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 172 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24762">
        <property type="entry name" value="TPR_IF140-IFT172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23387">
        <property type="entry name" value="TPR_IFT80_172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT80/172/WDR35 TPR" evidence="10">
        <location>
            <begin position="626"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="IF140/IFT172/WDR19 TPR" evidence="11">
        <location>
            <begin position="1032"/>
            <end position="1219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1748" mass="197950" checksum="74893036830B9ADE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUN8</accession>
    <name>A0A673AUN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative nuclease HARBI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Harbinger transposase-derived nuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">si:dkey-197c15.6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transposase-derived protein that may have nuclease activity. Does not have transposase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AUN8"/>
    <dbReference type="Ensembl" id="ENSSORT00005033953.1">
        <property type="protein sequence ID" value="ENSSORP00005033049.1"/>
        <property type="gene ID" value="ENSSORG00005015676.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUN8"/>
    <dbReference type="OrthoDB" id="9946389at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026103">
        <property type="entry name" value="HARBI1_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF252">
        <property type="entry name" value="NUCLEASE HARBI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02086">
        <property type="entry name" value="PUTNUCHARBI1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="14">
        <location>
            <begin position="156"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="370" mass="40773" checksum="7CF9B8135A871D02" modified="2020-06-17" version="1">MAFALPVWLAVHQELLAHEEVDRGAPTCFDEFDDESLFQLFHLTRPCITFITDTVRARMNKVVMKKPVVSVDSLVMVTLDYYAHGVSSASILDRVGLSQAHSPAIISLVSGTISGMVDQFISFPQTEEARANVAAKIEQICDIPNVLGIIAPAHFRVRASSYEDDYKSFVNTLGNTSVVSQLICDSDGNILSIEKCCVGGTSEQEMWESSFKGQEIEEDLHGPYWVIGGKGYHLSKHVLTPVPEPVSDSEACFNEAHAKIYRVIWTTIGSMKRRFKCLTQLGYAREGGLDKKSNIIKACSVLHNIAKKFSVPPPTGVGQTEPLYPGRQLSVTPGSNDEALKARQALIDSNFYVPPRSHYPSDSSSEEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AXQ5</accession>
    <name>A0A673AXQ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase C</fullName>
            <ecNumber evidence="17">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">prkcea</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A673AXQ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005034912.1">
        <property type="protein sequence ID" value="ENSSORP00005033999.1"/>
        <property type="gene ID" value="ENSSORG00005015574.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AXQ5"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20835">
        <property type="entry name" value="C1_nPKC_epsilon-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20838">
        <property type="entry name" value="C1_nPKC_epsilon-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04014">
        <property type="entry name" value="C2_PKC_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05591">
        <property type="entry name" value="STKc_nPKC_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000080">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000003">
        <property type="entry name" value="Protein kinase C delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000126">
        <property type="entry name" value="Protein kinase C epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000056">
        <property type="entry name" value="Protein kinase C epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000024">
        <property type="entry name" value="Protein kinase C epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034669">
        <property type="entry name" value="nPKC_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014376">
        <property type="entry name" value="Prot_kin_PKC_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000551">
        <property type="entry name" value="PKC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2" evidence="22">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
        <location>
            <begin position="170"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
        <location>
            <begin position="244"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="423"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="25">
        <location>
            <begin position="684"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="312"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="372"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="380"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="547"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="429"/>
            <end position="437"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19 20">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000551-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000551-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="753" mass="85023" checksum="F34CBFAC4779CAEE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2Q5</accession>
    <name>A0A673B2Q5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B2Q5"/>
    <dbReference type="Ensembl" id="ENSSORT00005036579.1">
        <property type="protein sequence ID" value="ENSSORP00005035629.1"/>
        <property type="gene ID" value="ENSSORG00005016820.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2Q5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="4">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="127" mass="14567" checksum="8A2E4CDB1CD3A469" modified="2020-06-17" version="1">SEFCLFLSVFKAGLWDMETEAVTETEEEGQDIEITCSHSNAIGNGKYFCKGDCRNKDVLIHTADKVPNGKYSIRDEGNTFYVTIFKLTINDSGLYWCGIDRVGRDTYNRVDLIVTKREFIHHCTFIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B7W0</accession>
    <name>A0A673B7W0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Antigen peptide transporter 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tap1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B7W0"/>
    <dbReference type="Ensembl" id="ENSSORT00005038403.1">
        <property type="protein sequence ID" value="ENSSORP00005037429.1"/>
        <property type="gene ID" value="ENSSORG00005017552.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B7W0"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18589">
        <property type="entry name" value="ABC_6TM_TAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000215">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000140">
        <property type="entry name" value="Lipid A export ATP-binding/permease protein MsbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF13">
        <property type="entry name" value="ANTIGEN PEPTIDE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01896">
        <property type="entry name" value="TAP1PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="173"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="488"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="742" mass="83669" checksum="9F6A2A76FD2CF905" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B913</accession>
    <name>A0A673B913_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B913"/>
    <dbReference type="Ensembl" id="ENSSORT00005038062.1">
        <property type="protein sequence ID" value="ENSSORP00005037092.1"/>
        <property type="gene ID" value="ENSSORG00005017416.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B913"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000004">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000011">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF374">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="6">
        <location>
            <begin position="23"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="6">
        <location>
            <begin position="79"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="6">
        <location>
            <begin position="135"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="241" mass="27307" checksum="5C07FBD65D0E7803" modified="2020-06-17" version="1">MDIISIYLCINFNYCALCFVEVCRLPPKTGPCRAYITRFFYNSTSMKCEVFVYGGCDGNRNNFGNMAQCKKACHMAEVCHLPPQTGSCNMYEDRYFYNSTSKSCNRFVYGGCDGNQNNFKHKANCMHRCQPEKVCNQPPGPGGGSGNLKRFFYNSTSRSCEVFIYRGCGGNQNRFRSKRECIHTCQPLVKCGGKPPPQKKQTYSVFSSPNCYLKWDTTCSESVAETIALHSIQNSRFFVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BBC8</accession>
    <name>A0A673BBC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BBC8"/>
    <dbReference type="Ensembl" id="ENSSORT00005040718.1">
        <property type="protein sequence ID" value="ENSSORP00005039706.1"/>
        <property type="gene ID" value="ENSSORG00005018527.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BBC8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000091">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="31"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="134"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="14">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24299" checksum="33FB53C7AE93CACF" modified="2020-06-17" version="1">NLINTMPEPAKSAPKKGSKKAVAKTAGKGGKKRRKGRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTGVRLLLPGELAKHAVSEGTKAVTKYTSSKLLRKGNYAQRVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTEKAGKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCT2</accession>
    <name>A0A673BCT2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BCT2"/>
    <dbReference type="FunCoup" id="A0A673BCT2">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039419.1">
        <property type="protein sequence ID" value="ENSSORP00005038427.1"/>
        <property type="gene ID" value="ENSSORG00005017951.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BCT2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="9">
        <location>
            <begin position="286"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="906"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1101" mass="124681" checksum="4984669E4389F89C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BDT2</accession>
    <name>A0A673BDT2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Free fatty acid receptor 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BDT2"/>
    <dbReference type="Ensembl" id="ENSSORT00005039541.1">
        <property type="protein sequence ID" value="ENSSORP00005038548.1"/>
        <property type="gene ID" value="ENSSORG00005018011.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BDT2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:peptide binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008527">
        <property type="term" value="F:taste receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046879">
        <property type="term" value="P:hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF67">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="47"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="348" mass="39807" checksum="2F00339548B6A3D5" modified="2020-06-17" version="1">SKTECAIDFQNFTYFSFFSEMYHTHRVVATAIETLVITSVFLVSVIANTGAAALVTWERRLLANKTILTLNLFVADLLFVSMIPLIVAVRWTVSWTLGYSACHTVLYVMCVSGCVTIVTLASISVERLQAILRLQTIPALNPRMVTTTLAFIWAFSALTAIPLSLFFTVMEDRVHICTLKWPDSTAEIAWNVAFTALCFLLPGIIIMCSYSKILQWTHLINGSLLLFNALMTKYSDYQLSYSPEYQISRQDIKLFRTLLVLVLSFLVMWSPIFILTFLILARNFSGHLYVTSTMFFWVVTFTMANSALNPILYSVFQFKHDWRKLCCGFIMTTIIQRFLLSIHYMHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BE20</accession>
    <name>A0A673BE20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adipolin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Adipose-derived insulin-sensitizing factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Complement C1q tumor necrosis factor-related protein 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">c1qtnf12</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adipolin/erythroferrone family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029994077.1">
        <property type="nucleotide sequence ID" value="XM_030138217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BE20"/>
    <dbReference type="FunCoup" id="A0A673BE20">
        <property type="interactions" value="1166"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041834.1">
        <property type="protein sequence ID" value="ENSSORP00005040781.1"/>
        <property type="gene ID" value="ENSSORG00005019036.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422130"/>
    <dbReference type="CTD" id="388581"/>
    <dbReference type="InParanoid" id="A0A673BE20"/>
    <dbReference type="OrthoDB" id="6431870at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000012">
        <property type="entry name" value="Adipolin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.320:FF:000006">
        <property type="entry name" value="Protein FAM132A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052136">
        <property type="entry name" value="Adipolin/Erythroferrone-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24019">
        <property type="entry name" value="ADIPOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24019:SF14">
        <property type="entry name" value="ADIPOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0372">Hormone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025650287" description="Adipolin" evidence="12">
        <location>
            <begin position="28"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="13">
        <location>
            <begin position="164"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="320" mass="34908" checksum="C89F87B6D922613F" modified="2020-06-17" version="1">MWCGAGPLAMVAAVFWTQCLLLEGVDAKKERKRPKEGTPQNTETYNATVSNSEEVGGNIKISDHQRVDPLGSWMDFVKRPVGNFPGKCRKRKRPLPGPPGPPGPPGPQGPPGSPGAEVTQEVLLQEFKEMIKEASERRAAALDRQTSPSQVPMALLTLEGMTSYRRIEEAFHCKLKGPVVVDKKTLVELQNFQTPPAKGAFLRGSGMDQSTGRFTAPITGIYQFSANVHIDHNEVKRSKSQLKARDNVRVLICIDSLCHRYTSLEMIVGLESNSKIFTVSVQGLLELQAGQYTSIFVDNAAGASITIQNGSDFMGMLLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BE75</accession>
    <name>A0A673BE75_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BE75"/>
    <dbReference type="Ensembl" id="ENSSORT00005040584.1">
        <property type="protein sequence ID" value="ENSSORP00005039574.1"/>
        <property type="gene ID" value="ENSSORG00005018449.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BE75"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="65" mass="7358" checksum="C980ABC99712A236" modified="2020-06-17" version="1">MSLSTARSFLSEVYYDEQELDANSALMELDKGMLGVHGCIRTLSAVYWIIPQVSNIRHLWVQSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BGJ8</accession>
    <name>A0A673BGJ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Insulin-degrading enzyme</fullName>
            <ecNumber evidence="20">3.4.24.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Insulin protease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Insulysin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">ide</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Degradation of insulin, glucagon and other polypeptides. No action on proteins.</text>
            <dbReference type="EC" id="3.4.24.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homodimer. Can also form homotetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.56" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A673BGJ8"/>
    <dbReference type="FunCoup" id="A0A673BGJ8">
        <property type="interactions" value="1891"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042756.1">
        <property type="protein sequence ID" value="ENSSORP00005041686.1"/>
        <property type="gene ID" value="ENSSORG00005018442.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BGJ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097242">
        <property type="term" value="P:amyloid-beta clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050435">
        <property type="term" value="P:amyloid-beta metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042447">
        <property type="term" value="P:hormone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000003">
        <property type="entry name" value="Insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000007">
        <property type="entry name" value="Insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000004">
        <property type="entry name" value="Putative insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000006">
        <property type="entry name" value="Putative insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="25">
        <location>
            <begin position="71"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="26">
        <location>
            <begin position="233"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="27">
        <location>
            <begin position="417"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="28">
        <location>
            <begin position="805"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00066874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00070422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1014" mass="117345" checksum="34BAA81416214959" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIN8</accession>
    <name>A0A673BIN8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 266</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115417116</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BIN8"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR042857">
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 266"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 266"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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            <end position="123"/>
        </location>
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        <location>
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            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="197"/>
        </location>
    </feature>
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        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="398"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="548" mass="60273" checksum="CDC52ED936EBCB3E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJC6</accession>
    <name>A0A673BJC6_9TELE</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nematocyst</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029997626.1">
        <property type="nucleotide sequence ID" value="XM_030141766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BJC6"/>
    <dbReference type="Ensembl" id="ENSSORT00005041760.1">
        <property type="protein sequence ID" value="ENSSORP00005040707.1"/>
        <property type="gene ID" value="ENSSORG00005019006.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424479"/>
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    <dbReference type="OrthoDB" id="2304600at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cytolysis in another organism"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:monoatomic cation transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cytolysin/lectin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Actinoporin_PFT"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1053">Target membrane</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="177" mass="19799" checksum="17FC2D7CCF337750" modified="2020-06-17" version="1">MPETAEAVSATLTTNRNCTIEITNVSGNYCLINPKVYMSSGFSQHPPQPTVRTTMSEVCTFTKDDNTATGAVGLLTYDLFHMQSRVCSERMAIMFSVPFDRNLYKNRLAIGVFEQSRACDKTLYDQMYDGKDLKNFVRSDANGSGLEYKATYVDLRATMSSVGKGIVKMELYDKMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJY9</accession>
    <name>A0A673BJY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transmembrane 4 superfamily member 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115433505</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM47 family.</text>
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    <dbReference type="RefSeq" id="XP_030010820.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BJY9"/>
    <dbReference type="Ensembl" id="ENSSORT00005042661.1">
        <property type="protein sequence ID" value="ENSSORP00005041594.1"/>
        <property type="gene ID" value="ENSSORG00005019341.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433505"/>
    <dbReference type="InParanoid" id="A0A673BJY9"/>
    <dbReference type="OrthoDB" id="8655982at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000010">
        <property type="entry name" value="transmembrane protein 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015664">
        <property type="entry name" value="P53_induced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399">
        <property type="entry name" value="P53-INDUCED PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="182" mass="20015" checksum="38591B70B7DD8DD4" modified="2020-06-17" version="1">MSSAVTETEESARSSPLTPLKLVGLVCVFLALCLDVGAVMSPAWVTADDQYYLSLWESCWKPASTESWQCSSTLGSDWQVATLALLLGGGALVLMSFLVALVAVCIGTRRRFYAPVAFMLFAAVVLQACSLVLYPIKFIESINLRIYHEFNWGYGLAWGGTIFSFGGGILYCLNPKNYEEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLQ0</accession>
    <name>A0A673BLQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thyroid hormone receptor associated protein 3b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">thrap3b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCLAF1/THRAP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BLQ0"/>
    <dbReference type="FunCoup" id="A0A673BLQ0">
        <property type="interactions" value="1909"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043801.1">
        <property type="protein sequence ID" value="ENSSORP00005042715.1"/>
        <property type="gene ID" value="ENSSORG00005019782.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLQ0"/>
    <dbReference type="OrthoDB" id="9948513at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029199">
        <property type="entry name" value="THRAP3_BCLAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15268">
        <property type="entry name" value="THRAP3/BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15268:SF16">
        <property type="entry name" value="THYROID HORMONE RECEPTOR-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15440">
        <property type="entry name" value="THRAP3_BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="654"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
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        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
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        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
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        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
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        <location>
            <begin position="316"/>
            <end position="346"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
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        <location>
            <begin position="654"/>
            <end position="664"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="699"/>
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        </location>
    </feature>
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        <location>
            <begin position="715"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
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        <location>
            <begin position="775"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="814"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="863"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="890" mass="100735" checksum="4DCF55F3E87E115D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMC6</accession>
    <name>A0A673BMC6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine N-acyltransferase-like protein</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">si:dkey-76k16.5</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673BMC6"/>
    <dbReference type="Ensembl" id="ENSSORT00005044015.1">
        <property type="protein sequence ID" value="ENSSORP00005042925.1"/>
        <property type="gene ID" value="ENSSORG00005019864.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BMC6"/>
    <dbReference type="OrthoDB" id="61870at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047961">
        <property type="term" value="F:glycine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010313">
        <property type="entry name" value="Glycine_N-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013652">
        <property type="entry name" value="Glycine_N-acyltransferase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015938">
        <property type="entry name" value="Glycine_N-acyltransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298:SF17">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298">
        <property type="entry name" value="L-COA N-ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08444">
        <property type="entry name" value="Gly_acyl_tr_C"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF06021">
        <property type="entry name" value="Gly_acyl_tr_N"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="150"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="278" mass="31905" checksum="9D513430A1D534F9" modified="2020-06-17" version="1">MKVLNKHELQIAEEVLLKHIPKSFKVYGFIYALNRNKPTTMEVVVDTWPDFKVIICRPDPQKKLTSELLRKVSFYCMDEQILRKMLTEENAVDFSTFFRFSGFDMSITGMIKEISSDRGINTRSLTVVNLLYSPDCSSLISPAVDSDLESRISSLNLSHVDLVNQTWKFGGTEQGYKNITNLISNFPSYCITDDQGQPVSWILVYDYCALGMLYTLPEHRGKGYAKVLINTMAKSLHAQGFPVYCFIEEENTISYRLFKSMGFIDVPSYRAQWLEINC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BME9</accession>
    <name>A0A673BME9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Prostaglandin E2 receptor EP2 subtype</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Prostanoid EP2 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ptger2a</name>
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    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BME9"/>
    <dbReference type="Ensembl" id="ENSSORT00005042844.1">
        <property type="protein sequence ID" value="ENSSORP00005041772.1"/>
        <property type="gene ID" value="ENSSORG00005019401.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BME9"/>
    <dbReference type="OrthoDB" id="5959154at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004957">
        <property type="term" value="F:prostaglandin E receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071380">
        <property type="term" value="P:cellular response to prostaglandin E stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000212">
        <property type="entry name" value="Prostaglandin E2 receptor EP2 subtype"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF8">
        <property type="entry name" value="PROSTAGLANDIN E2 RECEPTOR EP2 SUBTYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="218"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="56"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="400" mass="45236" checksum="1D35B53075D182BC" modified="2020-06-17" version="1">MLLKTSFESRDYFPIMSNNSNIPVETPEDICHDQHHVSSKRSPAISAAMFALGIFGNVAALVILEIRRRRDMKAGGTRMYGLFHVLIRILVVTDLSGTCMTSPVVQLSYSLNITLVGLMPDSRFLCEYFGFTMTFFSLTTLCILFTMAAERCLAIGYPYKYSQYITKKRGCIIIGCFFVASILFCVLPFIFNFGDFVQYCPGTWCFFDMNSTQKRHRIYATVYATIMLGLVLAIVVCNLFVVYQLFKMYQRRKRNGGSAMANVRPRSDRRVTSMAEEVEYLIQLVFMTIIFIICTVPLVVRVYINSAGDVNRKESHGLDLIALRFISVNSIIDPWVFILLSRNVVHCFWSSLCKLTLGGSRGSLFKLSVAPKENTPAQQDLTQPTVECTEPVTTQTVCNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMV8</accession>
    <name>A0A673BMV8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Solute carrier family 25 member 24, like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">slc25a24l</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BMV8"/>
    <dbReference type="Ensembl" id="ENSSORT00005044198.1">
        <property type="protein sequence ID" value="ENSSORP00005043105.1"/>
        <property type="gene ID" value="ENSSORG00005019943.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BMV8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000028">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="102"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="172"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="255"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="351"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="443" mass="49581" checksum="EF3019BDC971B8B7" modified="2020-06-17" version="1">MDQFRGLFVKLDQNNDGFISLAELHNEMKKHGILSADGKVQNIIDSYDKDKDGQLDYKEFLSYMMDREKKWKIHFHDLDKNKCGVIDQEDIICLFTELGVVISKPNAKKIIQMMDKDNSLTVDWSEFLHYVILNPVDNIGELVSSWKHSLVFDVGESRAMPIEFPEEASGFGAWRTFVLAAGLADAVSRTVTAPIDRLKTQLQVHGSKAFSQGFQEVRSGGLRSMWQGNAVNVLKGTPQSTLQCLIYAQVRHEALTVQQRFSLGCVSGAVAHAAFYPLEVLKVRLNLQRIGTYHGVMACAQTIYRNESLSSIYRGFKPSILCMIPYAGVECAVHQSIMNWAKSDPAYNSESKLFFFSFVAFASGQITSYPLAVIRTQQQAQAFNSNARATSSVLQGLIGIYENHGFRGYYSGMGASFVRAIPCALINYTLTRKFENVFSSVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXG5</accession>
    <name>A0A673BXG5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BXG5"/>
    <dbReference type="FunCoup" id="A0A673BXG5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047621.1">
        <property type="protein sequence ID" value="ENSSORP00005046460.1"/>
        <property type="gene ID" value="ENSSORG00005021269.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BXG5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19800">
        <property type="entry name" value="Bbox2_xNF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="193"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="463" mass="54015" checksum="FED846579A173EB0" modified="2020-06-17" version="1">MASASLSEDLTCSICLSVFTDPVILLCGHSFCRECITLSLDKQQHCPHCRTPVPTEGNYLLTNHILKSLAEKSKEAAKLKREYRNEKEVSDYNETEWFCLEHEEKLKLFCVTDQQLTCIICRDSERHEEHKFKPVKEAAASLRKDLDMFMLQVSADIRGLEGIANTQREEMIKTTESCNQLKTHINTQFEEMHMFLRKRQKEINDELKNKEEDALKKMRWRLNSLETALSENRELEGNVASILENKETERFLETWTDDDTTKTAKNTFKSKTENFQVEKASISLGPYESHLQFFVWKEMLQVIKPREEMLSLRNDDTSLCVSEDQRSLIFCNTKRSSDYYGRKPTQPYFTNSIDTFAGQHYWEIDVGELTSWELGIENNFLTMEGNTYEICIEDSYETLKFTGRPRRIGIYLNCSSKELSFYDADKMKHIETIRLSSMPSQVSAYFKVSQSNPDHTPLTVCWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BY64</accession>
    <name>A0A673BY64_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Eukaryotic elongation factor 2 kinase</fullName>
            <ecNumber evidence="10 12">2.7.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">eef2k</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[translation elongation factor 2] + ATP = [translation elongation factor 2]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21436"/>
            <dbReference type="Rhea" id="RHEA-COMP:11268"/>
            <dbReference type="Rhea" id="RHEA-COMP:11269"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.20"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer or homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 12">Belongs to the protein kinase superfamily. Alpha-type protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.20" evidence="10 12"/>
    <dbReference type="RefSeq" id="XP_029977906.1">
        <property type="nucleotide sequence ID" value="XM_030122046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BY64"/>
    <dbReference type="FunCoup" id="A0A673BY64">
        <property type="interactions" value="456"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046204.1">
        <property type="protein sequence ID" value="ENSSORP00005045068.1"/>
        <property type="gene ID" value="ENSSORG00005020700.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410406"/>
    <dbReference type="CTD" id="29904"/>
    <dbReference type="InParanoid" id="A0A673BY64"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004686">
        <property type="term" value="F:elongation factor-2 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903013">
        <property type="term" value="P:response to differentiation-inducing factor 1"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16967">
        <property type="entry name" value="Alpha_kinase_eEF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.200.10:FF:000002">
        <property type="entry name" value="Eukaryotic elongation factor 2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000230">
        <property type="entry name" value="Eukaryotic elongation factor 2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000336">
        <property type="entry name" value="Eukaryotic elongation factor 2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017400">
        <property type="entry name" value="eEF-2K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047588">
        <property type="entry name" value="eEF2K_a_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038139">
        <property type="entry name" value="Elongation_factor_2_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="14">
        <location>
            <begin position="133"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="437"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="459"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="736" mass="83474" checksum="1F9F00C426760208" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYP1</accession>
    <name>A0A673BYP1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cib1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029993393.1">
        <property type="nucleotide sequence ID" value="XM_030137533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BYP1"/>
    <dbReference type="Ensembl" id="ENSSORT00005047302.1">
        <property type="protein sequence ID" value="ENSSORP00005046144.1"/>
        <property type="gene ID" value="ENSSORG00005021143.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421587"/>
    <dbReference type="CTD" id="10519"/>
    <dbReference type="InParanoid" id="A0A673BYP1"/>
    <dbReference type="OrthoDB" id="114727at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000079">
        <property type="entry name" value="Calcium and integrin-binding family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051433">
        <property type="entry name" value="CIBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791:SF1">
        <property type="entry name" value="CALCIUM AND INTEGRIN BINDING FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791">
        <property type="entry name" value="CALCIUM AND INTEGRIN BINDING FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="146"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="189" mass="21476" checksum="1902F4B263F76A30" modified="2020-06-17" version="1">MGTTASQLGKDLLSEYQELTFLTKQEILLAHKRFTELLTKDERDGANSRLPMDRILTLPELKSNPFRERICHVFSTSPQKDGSLTFEDFLDLLSAFSDSATLEIKSHYAFRIFDFDDDGTLDGSDLEKLVNCLTGETDETRLSPEEMRQLISNILEESDIDKDGTVNLSEFQHVISRSPDFVSSFKIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C001</accession>
    <name>A0A673C001_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Chitotriosidase-1</fullName>
            <ecNumber evidence="6">3.2.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Chitinase-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115419703</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673C001"/>
    <dbReference type="Ensembl" id="ENSSORT00005047421.1">
        <property type="protein sequence ID" value="ENSSORP00005046262.1"/>
        <property type="gene ID" value="ENSSORG00005021184.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C001"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02872">
        <property type="entry name" value="GH18_chitolectin_chitotriosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.140.10:FF:000001">
        <property type="entry name" value="Acidic mammalian chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000081">
        <property type="entry name" value="Chitinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000001">
        <property type="entry name" value="Chitinase 3-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000220">
        <property type="entry name" value="Chitotriosidase-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF188">
        <property type="entry name" value="ACIDIC MAMMALIAN CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="8" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH18" evidence="21">
        <location>
            <begin position="15"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="20">
        <location>
            <begin position="427"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="386"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="390"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="476" mass="51816" checksum="07A615CCB01DFD9D" modified="2020-06-17" version="1">MGKLLICVGKRRSSYILSCYFTNWGQYRPGAGKYFPTNVDPCLCDHLIYAFAGMGNNMIKTYEWDDEKLYAQFQALKNQNSNLKTLLAIGGWNFGTAKFTAMVSSPANRQTFIDSVIKFLRQYQFDGLDIDWEYPGSRGSPPQDKERFTVLVQELMSAFEAEGKKTNRPRLMLTAAVSAGKGTIDSGYQIAQIGAVLDYFHVMTYDFHGSWEHNVGENSPLYKGPADQGAMIYFNVDYAMNYWKSNGAPAEKLLVGFPTYGHTFRLASSNTAVGAPASGPGPAGPFTRQAGFWAYYEICTFLKQGATQAWDAPQEVPYAYHQGTWVGYDNVKSFQNKIQWLKQSGFGGAMVWSLDLDDFSGTFCGQGKYPLINTIKSGLGTGTSCTAPINPNPPVTPTHQPPPQPGGGGSSGGGSSSGGSSGGSTGSGFCAGKANGLYPDPTDKNHFYECDQGQTYLQHCAVGLVFDDSCKCCNWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1V9</accession>
    <name>A0A673C1V9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Atypical kinase COQ8A, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Chaperone activity of bc1 complex-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Coenzyme Q protein 8A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">aarF domain-containing protein kinase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">coq8aa</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is still unclear: may act as a protein kinase that mediates phosphorylation of COQ3. According to other reports, acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates. However, the small molecule kinase activity was not confirmed by another publication. Shows an unusual selectivity for binding ADP over ATP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C1V9"/>
    <dbReference type="FunCoup" id="A0A673C1V9">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048103.1">
        <property type="protein sequence ID" value="ENSSORP00005046932.1"/>
        <property type="gene ID" value="ENSSORG00005019538.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1V9"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF1">
        <property type="entry name" value="ATYPICAL KINASE COQ8A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="20">
        <location>
            <begin position="312"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="86"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="663" mass="74282" checksum="012E0D3B47117F45" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3M0</accession>
    <name>A0A673C3M0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="5">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cnot8</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="5"/>
    <dbReference type="RefSeq" id="XP_030010771.1">
        <property type="nucleotide sequence ID" value="XM_030154911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C3M0"/>
    <dbReference type="FunCoup" id="A0A673C3M0">
        <property type="interactions" value="1948"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051154.1">
        <property type="protein sequence ID" value="ENSSORP00005049951.1"/>
        <property type="gene ID" value="ENSSORG00005022664.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433482"/>
    <dbReference type="CTD" id="9337"/>
    <dbReference type="InParanoid" id="A0A673C3M0"/>
    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000005">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="285" mass="32678" checksum="8B23B0CF1E9E706D" modified="2020-06-17" version="1">MPAALTDSSQIICEVWASNVEDEMRKIRQIIQSYNYIAMDTEFPGVVVRPIGEFRSTVDYQYQLLRCNVDLLKIIQLGLTFMNEDGDYPPGTTTWQFNFKFNLTEDMYSQDSIDLLQNSGLQFKKHEEEGIDTLYFAELLMTSGLVLCENVKWLSFHSGYDFGYLVKLLTDARLPEEEHDFFQILNLFFPAIYDVKYLMKSCKNLKGGLQEVADQLELKRIGRQHQAGSDSLLTGMAFFRMKELFFEDNIDDAKYCGRLYGLGSGSSQPQNGISSSGQEETNNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6H3</accession>
    <name>A0A673C6H3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sec1 family domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C6H3"/>
    <dbReference type="Ensembl" id="ENSSORT00005052290.1">
        <property type="protein sequence ID" value="ENSSORP00005051071.1"/>
        <property type="gene ID" value="ENSSORG00005023084.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C6H3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000002">
        <property type="entry name" value="Sec1 family domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="637"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="652"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="668" mass="75105" checksum="F2E4A57EEC1B12FF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6Y4</accession>
    <name>A0A673C6Y4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Multiple C2 and transmembrane domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mctp2a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCTP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C6Y4"/>
    <dbReference type="Ensembl" id="ENSSORT00005049779.1">
        <property type="protein sequence ID" value="ENSSORP00005048587.1"/>
        <property type="gene ID" value="ENSSORG00005022137.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C6Y4"/>
    <dbReference type="OrthoDB" id="5973539at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08376">
        <property type="entry name" value="C2B_MCTP_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08377">
        <property type="entry name" value="C2C_MCTP_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000019">
        <property type="entry name" value="Multiple C2 and transmembrane domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000174">
        <property type="entry name" value="Multiple C2 domains, transmembrane 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000363">
        <property type="entry name" value="Multiple C2 domains, transmembrane 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013583">
        <property type="entry name" value="MCTP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911:SF9">
        <property type="entry name" value="MULTIPLE C2 AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08372">
        <property type="entry name" value="PRT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="876"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="977"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="361"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="522"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="678"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1059" mass="120978" checksum="2A95AC5376250BB5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7E3</accession>
    <name>A0A673C7E3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">R-spondin 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the R-spondin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C7E3"/>
    <dbReference type="Ensembl" id="ENSSORT00005052669.1">
        <property type="protein sequence ID" value="ENSSORP00005051441.1"/>
        <property type="gene ID" value="ENSSORG00005023243.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C7E3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051514">
        <property type="entry name" value="R-spondin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043601">
        <property type="entry name" value="Rspo_Fu-CRD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46987">
        <property type="entry name" value="NEUROHYPOPHYSIAL HORMONES, N-TERMINAL DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46987:SF1">
        <property type="entry name" value="R-SPONDIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15913">
        <property type="entry name" value="Furin-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="R-spondin Fu-CRD" evidence="10">
        <location>
            <begin position="35"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="199" mass="22623" checksum="A535759E20112F5E" modified="2020-06-17" version="1">NVPQLLFFESLIVNCRQILHMEAISNSSVSRLCPAGCATCSALNGCLSCKPRLFFHLELDGMRQRGTCLSSCPRGHYGMRSPYINTCTKCRDDCASCFSENFCTRCHLGHFLFRGRCENSCPNGLTADTELRECTVHCEVGEWTEWSPCARKRSMRAYRRGEETRTREVLHSPSLYGDPCPHVSEIRKCVIKKRQSLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9T5</accession>
    <name>A0A673C9T5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">10-formyltetrahydrofolate dehydrogenase</fullName>
            <ecNumber evidence="9">1.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">aldh1l2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-10-formyltetrahydrofolate + NADP(+) + H2O = (6S)-5,6,7,8-tetrahydrofolate + CO2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10180"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="1.5.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10181"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">In the N-terminal section; belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.6" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673C9T5"/>
    <dbReference type="Ensembl" id="ENSSORT00005050749.1">
        <property type="protein sequence ID" value="ENSSORP00005049547.1"/>
        <property type="gene ID" value="ENSSORG00005022117.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C9T5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016155">
        <property type="term" value="F:formyltetrahydrofolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009258">
        <property type="term" value="P:10-formyltetrahydrofolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07140">
        <property type="entry name" value="ALDH_F1L_FTFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08703">
        <property type="entry name" value="FDH_Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08647">
        <property type="entry name" value="FMT_core_FDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.25.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000008">
        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000009">
        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="entry name" value="Formyl transferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011407">
        <property type="entry name" value="10_FTHF_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036489">
        <property type="entry name" value="10-FTHFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 12" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025480063" description="10-formyltetrahydrofolate dehydrogenase" evidence="16">
        <location>
            <begin position="16"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="17">
        <location>
            <begin position="346"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="735"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="599"/>
            <end position="601"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="625"/>
            <end position="628"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="658"/>
            <end position="663"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="678"/>
            <end position="679"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="785"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="832"/>
            <end position="834"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="13">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="926" mass="101474" checksum="502DA68F19307A8F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CA86</accession>
    <name>A0A673CA86_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aryl hydrocarbon receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115429938</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030005631.1">
        <property type="nucleotide sequence ID" value="XM_030149771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CA86"/>
    <dbReference type="Ensembl" id="ENSSORT00005052156.1">
        <property type="protein sequence ID" value="ENSSORP00005050940.1"/>
        <property type="gene ID" value="ENSSORG00005023049.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429938"/>
    <dbReference type="InParanoid" id="A0A673CA86"/>
    <dbReference type="OrthoDB" id="6099906at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034751">
        <property type="term" value="C:aryl hydrocarbon receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19696">
        <property type="entry name" value="bHLH-PAS_AhR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000035">
        <property type="entry name" value="Aryl hydrocarbon receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000019">
        <property type="entry name" value="Aryl hydrocarbon receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039091">
        <property type="entry name" value="AHR/AHRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10649">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10649:SF17">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="22"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="102"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="757"/>
            <end position="811"/>
        </location>
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        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="776"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="800"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="922" mass="101635" checksum="EEACF2D47D7861B2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CD75</accession>
    <name>A0A673CD75_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">aurkaip1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the mitochondrion-specific ribosomal protein mS38 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993739.1">
        <property type="nucleotide sequence ID" value="XM_030137879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CD75"/>
    <dbReference type="FunCoup" id="A0A673CD75">
        <property type="interactions" value="1370"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054777.1">
        <property type="protein sequence ID" value="ENSSORP00005053521.1"/>
        <property type="gene ID" value="ENSSORG00005024071.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421881"/>
    <dbReference type="CTD" id="54998"/>
    <dbReference type="InParanoid" id="A0A673CD75"/>
    <dbReference type="OrthoDB" id="6139741at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013177">
        <property type="entry name" value="Ribosomal_mS38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035">
        <property type="entry name" value="AURORA KINASE A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32035:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08213">
        <property type="entry name" value="COX24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01155">
        <property type="entry name" value="DUF1713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein mS38 C-terminal" evidence="5">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="208" mass="24291" checksum="B1BAA57C352A3D19" modified="2020-06-17" version="1">MFTSKFLSRLGLLHRATCSLQTCGEIFRSPVQRVLPAYCSSNKERNYSTAADNTSPPRWVQLEPELDEALVPRKMSVSPLESWLSLRYSLPPLLESAPPQEDLEVLEEKVLPPILVPVLEDGEGSVTPLKCKNDIEIRRRKMNRHKYKKLQKRTKFLRRRVLEGRGRRKQKRFEEDLKRIWTRAGLKKAPQGWTTPKIFLKRHGNKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CDK0</accession>
    <name>A0A673CDK0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CDK0"/>
    <dbReference type="FunCoup" id="A0A673CDK0">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051387.1">
        <property type="protein sequence ID" value="ENSSORP00005050178.1"/>
        <property type="gene ID" value="ENSSORG00005022769.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CDK0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF29">
        <property type="entry name" value="KINESIN FAMILY MEMBER 20B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="487"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="696" mass="79066" checksum="F675F5E9CDD83C32" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CE26</accession>
    <name>A0A673CE26_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CE26"/>
    <dbReference type="Ensembl" id="ENSSORT00005055103.1">
        <property type="protein sequence ID" value="ENSSORP00005053836.1"/>
        <property type="gene ID" value="ENSSORG00005024170.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CE26"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17745">
        <property type="entry name" value="BRCT_p53bp1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000003">
        <property type="entry name" value="Tumor suppressor p53-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015125">
        <property type="entry name" value="53-BP1_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047249">
        <property type="entry name" value="BRCT_p53bp1-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047252">
        <property type="entry name" value="TP53BP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321:SF3">
        <property type="entry name" value="TP53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15321">
        <property type="entry name" value="TUMOR SUPPRESSOR P53-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09038">
        <property type="entry name" value="53-BP1_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="137"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="24"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="308"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="337" mass="36333" checksum="B4D4361757EB4D87" modified="2020-06-17" version="1">MGQWYNRTSVILSLDQGNRLREQHSLGPYEPATPLAKASDISLDNLVEGKRRRRGAPDTPTRTSSSSSSPRTPGPSGKRRLMSPEANRTGRTPPKRGRRGGHRSGTKRLGVCNTSGSGTDGAGPSHDPADTHGPLPPNASLFMGFAFMLTASSESDRLTNRPSSDGDEGEDDYVQTGPYNKTYTEAQLQAGGGFVLPDFNEEQCKAAYQSLLIADQHCRTRKYLLCVASGVPCVSHMWVRDCCKENKLLNYRNYLLPAGAGPDGAIVEWHPRSSPFKALKVLLVFQEPPELWAQLITLGGGATVRQLHPDKDGSGMDTGDQEPEPGCGLVLGSGKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CEU8</accession>
    <name>A0A673CEU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transcriptional repressor NF-X1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nuclear transcription factor, X box-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">nfx1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CEU8"/>
    <dbReference type="FunCoup" id="A0A673CEU8">
        <property type="interactions" value="2123"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053077.1">
        <property type="protein sequence ID" value="ENSSORP00005051839.1"/>
        <property type="gene ID" value="ENSSORG00005022997.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CEU8"/>
    <dbReference type="OrthoDB" id="6512771at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd02643">
        <property type="entry name" value="R3H_NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16696">
        <property type="entry name" value="RING-CH-C4HC3_NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000003">
        <property type="entry name" value="Transcriptional repressor NF-X1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034076">
        <property type="entry name" value="R3H_NF-X1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF12">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="318"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="321"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="R3H" evidence="22">
        <location>
            <begin position="958"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="951"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="132"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1085" mass="120761" checksum="9FEEA20C051729E5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFS2</accession>
    <name>A0A673CFS2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">txn2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CFS2"/>
    <dbReference type="FunCoup" id="A0A673CFS2">
        <property type="interactions" value="1136"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054224.1">
        <property type="protein sequence ID" value="ENSSORP00005052970.1"/>
        <property type="gene ID" value="ENSSORG00005023865.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CFS2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43601:SF3">
        <property type="entry name" value="THIOREDOXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="27"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="165" mass="18059" checksum="399B699CF70CCA58" modified="2020-06-17" version="1">MAHRLLLSRIWTLSAKDFRRVPASTATFASSSFPTSSLQSTTTRVSFLSPSRTLPHSLPHTSRREVSFNVQDHEDFTDGSSIVTCLWCGPCKILGPRLEKAVRKQKGRVAMAKVDIDDHTDLALEYGVSAVPTVIGMRGGDVVGHFVGIKDDDELDSFVSKVIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CHW6</accession>
    <name>A0A673CHW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 44</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CHW6"/>
    <dbReference type="Ensembl" id="ENSSORT00005055037.1">
        <property type="protein sequence ID" value="ENSSORP00005053770.1"/>
        <property type="gene ID" value="ENSSORG00005024157.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CHW6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF53">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025638883" description="Transmembrane protein 44" evidence="2">
        <location>
            <begin position="22"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="33104" checksum="85CD3B1F123BD471" modified="2020-06-17" version="1">MYSLVFTLFCFVLFCFSMVLAATDKHPCLLQISVSSSTLVYLQVPPNLLCLMFKNKYDGTSIDLFSEHLHLLLFEMFFQQARSDILGYTLGILSFVIACTSRFPTLHRGHTLPRAHAVSVVMRSLSGAFYAAAILLSDTHYAFLLRVMPWLLSAVSGIILDLLVSFCRTINTLYSLTLSSQMKSFLKATDMGRYIDVIAQPVRKICLKEISVSKETEDRSANGVERLIRVDSFYSDTSLDSSTVGSDLEVRTGTEKIKKRKPLQELKRKKTSARIKKIYLKDLNEYSWLSGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJ06</accession>
    <name>A0A673CJ06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">A kinase (PRKA) anchor protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJ06"/>
    <dbReference type="Ensembl" id="ENSSORT00005053283.1">
        <property type="protein sequence ID" value="ENSSORP00005052043.1"/>
        <property type="gene ID" value="ENSSORG00005023485.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJ06"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF2">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="184"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1011" mass="114330" checksum="02456B46770D854C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CL03</accession>
    <name>A0A673CL03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tmtc4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMTC family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673CL03"/>
    <dbReference type="FunCoup" id="A0A673CL03">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057592.1">
        <property type="protein sequence ID" value="ENSSORP00005056296.1"/>
        <property type="gene ID" value="ENSSORG00005025033.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CL03"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052346">
        <property type="entry name" value="O-mannosyl-transferase_TMTC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013618">
        <property type="entry name" value="TMTC_DUF1736"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44227">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44227:SF3">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08409">
        <property type="entry name" value="TMTC_DUF1736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="439"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1736" evidence="15">
        <location>
            <begin position="289"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="517"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="551"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="585"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="653"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="687"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="742" mass="83809" checksum="73D52DAA7594040B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CM05</accession>
    <name>A0A673CM05_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SET binding factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sbf1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A673CM05">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054322.1">
        <property type="protein sequence ID" value="ENSSORP00005053068.1"/>
        <property type="gene ID" value="ENSSORG00005022982.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CM05"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13340">
        <property type="entry name" value="PH-GRAM_MTMR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01235">
        <property type="entry name" value="PH_Sbf1_hMTMR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000006">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022096">
        <property type="entry name" value="SBF1/SBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF43">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12335">
        <property type="entry name" value="SBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025458079" evidence="4">
        <location>
            <begin position="20"/>
            <end position="1846"/>
        </location>
    </feature>
    <feature type="domain" description="UDENN" evidence="6">
        <location>
            <begin position="9"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Myotubularin phosphatase" evidence="7">
        <location>
            <begin position="1097"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="1740"/>
            <end position="1844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1262"/>
            <end position="1283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1846" mass="208176" checksum="4B9B6E3BD6B11DCD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CPH5</accession>
    <name>A0A673CPH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tetratricopeptide repeat protein 28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ttc28</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">During mitosis, may be involved in the condensation of spindle midzone microtubules, leading to the formation of midbody.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with AURKB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005056524.1">
        <property type="protein sequence ID" value="ENSSORP00005055244.1"/>
        <property type="gene ID" value="ENSSORG00005024668.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CPH5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000040">
        <property type="entry name" value="Tetratricopeptide repeat domain 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000056">
        <property type="entry name" value="Tetratricopeptide repeat domain 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000096">
        <property type="entry name" value="Tetratricopeptide repeat domain 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000209">
        <property type="entry name" value="Tetratricopeptide repeat domain 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000260">
        <property type="entry name" value="tetratricopeptide repeat protein 28 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024983">
        <property type="entry name" value="CHAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058900">
        <property type="entry name" value="TTC28_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098">
        <property type="entry name" value="RAPSYN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098:SF108">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12770">
        <property type="entry name" value="CHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26117">
        <property type="entry name" value="TTC28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 16" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="16">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="16">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="16">
        <location>
            <begin position="571"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="16">
        <location>
            <begin position="854"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="16">
        <location>
            <begin position="935"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="16">
        <location>
            <begin position="975"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="CHAT" evidence="18">
        <location>
            <begin position="1369"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="domain" description="TTC28 C-terminal" evidence="19">
        <location>
            <begin position="1831"/>
            <end position="1938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1569"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2016"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2265"/>
            <end position="2312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2358"/>
            <end position="2430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1570"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="2018"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="2100"/>
            <end position="2109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="2113"/>
            <end position="2125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2144"/>
            <end position="2158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2200"/>
            <end position="2232"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2273"/>
            <end position="2297"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="2298"/>
            <end position="2308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="2361"/>
            <end position="2379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF26117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2430" mass="263992" checksum="EEF942343B9E9F4B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CPM4</accession>
    <name>A0A673CPM4_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pgap3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the fatty acid remodeling steps of GPI-anchor maturation where the unsaturated acyl chain at sn-2 of inositol phosphate is replaced by a saturated stearoyl chain. May catalyze the first step of the fatty acid remodeling, by removing the unsaturated acyl chain at sn-2 of inositol phosphate, generating a lyso-GPI intermediate. The fatty acid remodeling steps is critical for the integration of GPI-APs into lipid rafts.</text>
    </comment>
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        <text evidence="9">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
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        <subcellularLocation>
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        <subcellularLocation>
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            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PGAP3 family.</text>
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        <property type="component" value="Chromosome 19"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
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        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3 9" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025527927" description="Post-GPI attachment to proteins factor 3" evidence="11">
        <location>
            <begin position="51"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="347" mass="39691" checksum="C9A918B6C8A79F87" modified="2020-06-17" version="1">MPQSPRRAFLSPDGPRPTAMASPSLRCTSVRLPALVLLLVSVTTVQSSQGDKEPVYRDCVKQCVRTNCTGARLRGFQSAQPQYMALTGWTCRDDCRYQCMWTTVGLYQAEGYRVPQFHGKWPFARFLCFEEPASALASLLNGLACLLMLLRYRSTVPRQSPMYHTINAFSLVSLNAWFWSTVFHTRDTYLTEKMDYFCATAVILYSIYLCCVRTLGLRRPGVSSMVGVLLILAFTSHVSYLTFISFDYGYNMAANASIGMVNLLWWLCWCWQNRRTLPYWWKCGLVVLLLHGLALLELLDFPPLLWVLDAHAVWHLSTIPVHFLFYSFLIDDSLYLLNTEKMGVKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CUI5</accession>
    <name>A0A673CUI5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CUI5"/>
    <dbReference type="Ensembl" id="ENSSORT00005058382.1">
        <property type="protein sequence ID" value="ENSSORP00005057079.1"/>
        <property type="gene ID" value="ENSSORG00005025337.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CUI5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="274" mass="30972" checksum="E2D9F92A0922A11D" modified="2020-06-17" version="1">MEEGTFEDQLLLHAKEGSCSHIQRKLFMLLSVMAARWKPNSGWTSLHLASYLGYRDVVEELLKAGADVNLQDNMGDTPLHKAACTGRKEIVLLLLRYDACVNIINGTAQIPKDVTEDDEIITMLEAAERREARRREEKLLEAAREGDVCTLSKLLSGEEAPNIQCRDALGNTPLHCAAYRGQKQCIIKLLKSGASPSLKNNNVQKIEGPLWKSSRFLGWRSHWVVIEDGSISWYPRQVYLFSILILLQPSESCMDNSVRYDYDINMCLVSKQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4G5</accession>
    <name>A0A672Y4G5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nuclear factor of activated T cells 3b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nfatc3b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y4G5"/>
    <dbReference type="Ensembl" id="ENSSORT00005001252.1">
        <property type="protein sequence ID" value="ENSSORP00005001217.1"/>
        <property type="gene ID" value="ENSSORG00005000642.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4G5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF6">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="11">
        <location>
            <begin position="398"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="240"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="733"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="780"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="780"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="860" mass="93726" checksum="C2ED12094A3C39A6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y7G3</accession>
    <name>A0A672Y7G3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coiled-coil domain containing 40</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ccdc40</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y7G3"/>
    <dbReference type="FunCoup" id="A0A672Y7G3">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002669.1">
        <property type="protein sequence ID" value="ENSSORP00005002602.1"/>
        <property type="gene ID" value="ENSSORG00005001558.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y7G3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037386">
        <property type="entry name" value="CCDC40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16275">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16275:SF8">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08647">
        <property type="entry name" value="BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="351"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="411"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="536"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="807" mass="93096" checksum="FFAFFA869B3A4859" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y825</accession>
    <name>A0A672Y825_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">STEAP family member 2, metalloreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115411392</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2 Cu(+) + NADP(+) + H(+) = 2 Cu(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71771"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:71773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 Fe(2+) + NADP(+) + H(+) = 2 Fe(3+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:71769"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the STEAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y825"/>
    <dbReference type="Ensembl" id="ENSSORT00005002851.1">
        <property type="protein sequence ID" value="ENSSORP00005002768.1"/>
        <property type="gene ID" value="ENSSORG00005001715.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y825"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000051">
        <property type="entry name" value="STEAP2 metalloreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF6">
        <property type="entry name" value="METALLOREDUCTASE STEAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="20">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="342" mass="39163" checksum="0FAAB24E166AE680" modified="2020-06-17" version="1">TCKTCWSVDRPVQPTVAVLGSGDFSRSLTMRLLHRGFHVVVGSRQPKMAAQSFPHVVDVTHHEDAVAKAAIVFLAIRREHYPVLWDLKHLLEGKILVDVSNNRRVNQYPESNAEYLASLLPQSTVVKGFNVISAWAMQQTSPMDASTQVFICSNSAQARQQIVDLARQLHFQPVDMGTLSSSRDIENMPIQLFPGWKGPVLAAVALSIFFFSYSFVRDIIHPYIKRKQSDFYKIPMEMVNRTLPTVAITLLALVYLGGQLAAAHQLYYGTKYRHFPQWLEGWLQSRKQLGLLSFFMAAVHVLYSVCLPMRRSERYLLLNTAYQQVRRERGREGERERERENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8J7</accession>
    <name>A0A672Y8J7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">microsomal epoxide hydrolase</fullName>
            <ecNumber evidence="4">3.3.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115415293</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-{[(4-methoxybenzyl)(methyl)amino]methyl}-2-methylpropane-1,3-diol</text>
            <dbReference type="Rhea" id="RHEA:55764"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:139161"/>
            <dbReference type="ChEBI" id="CHEBI:139164"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.9" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y8J7"/>
    <dbReference type="Ensembl" id="ENSSORT00005003162.1">
        <property type="protein sequence ID" value="ENSSORP00005003068.1"/>
        <property type="gene ID" value="ENSSORG00005001896.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003165.1">
        <property type="protein sequence ID" value="ENSSORP00005003070.1"/>
        <property type="gene ID" value="ENSSORG00005001896.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF70">
        <property type="entry name" value="EPOXIDE HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="9">
        <location>
            <begin position="75"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="484" mass="54852" checksum="C32D4A50FAEA004A" modified="2020-06-17" version="1">MKTNPITELLSSVCRPLVPVLVQTLGTMFTEILVSLLIGGLIYFLVMRNRNQVLKTKDGWWGAGAPPDGEEDVSVRPFKISTSDEELEDLHRRLDQTRPVASLEDSQFHYGFNSQCLQKVVSYWRNDFDWRKQVDKLNQYPHFKTNIEGIDVHYMHIKPQRVPQGSAVIPLIMVHGWPGSFYEFFGIIPLLTEPSDPGDCVFEVVCPSIPGYGFSEAPHKKGFDTVCAARIFIKLMKRLGFQHFYAQGGDWGWQVTTNMAQLEPKMVTGLHLNFASVTMGGLIVTLSIMFGRHFPKPFGFTDLDIQRLYPFMDKLVVESIKESGYMHIQATKPDTAGRGLNDSPVGLAAYILEKFSTATNFDFRNLEDGGLTRKFSLDDLLTNIMIYWTSGCIVSSMRFYKENVGHGLAPPHTKIPVVVPTGFACFPNEVMHMPKLWVQQKYHNLVSYTLMARGGHFAAMEEPQLLAEDIHKFVRTVEKKTKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBQ0</accession>
    <name>A0A672YBQ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neuronal pentraxin-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nptx1l</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YBQ0"/>
    <dbReference type="FunCoup" id="A0A672YBQ0">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000908.1">
        <property type="protein sequence ID" value="ENSSORP00005000880.1"/>
        <property type="gene ID" value="ENSSORG00005000539.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YBQ0"/>
    <dbReference type="OrthoDB" id="8871962at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00152">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000012">
        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF24">
        <property type="entry name" value="NEURONAL PENTRAXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025544137" evidence="9">
        <location>
            <begin position="25"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="10">
        <location>
            <begin position="226"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="127"/>
        </location>
    </feature>
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        <location>
            <begin position="130"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="432" mass="48352" checksum="C253572A7F343B14" modified="2020-06-17" version="1">MQGTKQGIYWRLFLVSCLFLESSSQDFGGQTQFICTSVPKDMDICAATLQNSVPGEDLKTTVMQLRETVLQQKETIMNQKETIRELTSKLARCESQSGIEPGDARPGGRRKEAGTKNTMGDVSRGPADTLTQLSQTLQSLKQRLENLEQFSRSNNSVQANSLKDLLQSKIDDLEKQVLSRVNSIEEGKPGLRNETEQRGRVESTLTSLHQRITDLEKGQRENRPLDKFQLTFPLRTNYMYAKVKKSLPEMYALTVCMWLKSNASPGVGTPFSYAVPGQANELVLIEWGNNPMEILINDKVAKLPFLINDGKWHHICVTWTTRDGVWEAFQDGVKRGSGENLAPYHPIKPQGLLILGQEQDMLGGGFDATQAFVGDLANFHIWDRKLSVGEIYNLATCSSKAQVGNVFAWMETSLDIYGGASKWTFEACRQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCY0</accession>
    <name>A0A672YCY0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lathosterol oxidase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115421395</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029993140.1">
        <property type="nucleotide sequence ID" value="XM_030137280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YCY0"/>
    <dbReference type="Ensembl" id="ENSSORT00005000737.1">
        <property type="protein sequence ID" value="ENSSORP00005000711.1"/>
        <property type="gene ID" value="ENSSORG00005000456.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421395"/>
    <dbReference type="InParanoid" id="A0A672YCY0"/>
    <dbReference type="OrthoDB" id="408954at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
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        <location>
            <begin position="125"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="278" mass="32909" checksum="09A89161FBFD2E4F" modified="2020-06-17" version="1">MDHVLNSADYYIFTPYVYPTSWPEDEALRQIISLWVMTTLGAELLYLVFGALNFYFVFDHKLMKHPQFIEKQMRKEIQLGTVSIFWMSFPTVALFYWEVMGYSKLFDDVSGSSQGWSFMFLSIAGFLFFTDMCIYWIHRVMHHKSIYKYLHKQHHLFKIPTPFASHAFHPLDGFLQSLPYHVYPFIFPLSKVVYLSLFIFVNIWTISIHDGDYRIPGPFIHLINGAAHHVDHHLYFNYNYGQYFTLWDRLGGSYRYPSALLGKGPHDHIKKLMAEKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YEM9</accession>
    <name>A0A672YEM9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Apolipoprotein A-II</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YEM9"/>
    <dbReference type="FunCoup" id="A0A672YEM9">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001921.1">
        <property type="protein sequence ID" value="ENSSORP00005001866.1"/>
        <property type="gene ID" value="ENSSORG00005001172.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YEM9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025585199" evidence="1">
        <location>
            <begin position="21"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="139" mass="15771" checksum="C98C302A09984F4C" modified="2020-06-17" version="1">MNAKYAIALILTLQVSLSLCDIPAPSPELVEKYDNYKRVFYQRLQNAYNNLMERVEQAKQNEHGQAVMSYVDSAKANPRVGAAIELIKGAHEELQPAIDEARTKVLGAYEHYLRPQVGQFLNDAIENIRTYLNVYMPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YG20</accession>
    <name>A0A672YG20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">eno1b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A672YG20"/>
    <dbReference type="FunCoup" id="A0A672YG20">
        <property type="interactions" value="496"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003608.1">
        <property type="protein sequence ID" value="ENSSORP00005003507.1"/>
        <property type="gene ID" value="ENSSORG00005002092.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YG20"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF55">
        <property type="entry name" value="ALPHA-ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase N-terminal" evidence="14">
        <location>
            <begin position="36"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="13">
        <location>
            <begin position="175"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="403"/>
            <end position="406"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="466" mass="51017" checksum="165AE63351D0F784" modified="2020-06-17" version="1">MCQNLFKQSVTICLSAMALVYVSFFFLVSRSYIMPIVKIHAREIFDSRGNPTVEVDLYTDKGLFRAAVPSGASTGIYEALELRDNDKSRYLGKGVSQAVENINSIIAPALVGQDISVVEQESIDQMMLDLDGTENKSKLGANAILGVSLAVCKAGAAEKEVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASTFKEAMRIGAEVYHNLKGVIKKKYGQDATNVGDEGGFAPNILENQEALELLKEAISKAGYTDEVVIGMDVAASEFYREGKYDLDFKSPDDPSRYITPDELADLYKSFVKDYPVVSIEDPFDQDDWAAWTNFTETTEIQVVGDDLTVTNPNRICKAVEEKACNCLLLKVNQIGTVTESMKACKMAQESGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGDKALFAGKNFRKPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGL2</accession>
    <name>A0A672YGL2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Sushi, nidogen and EGF-like domains 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">sned1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A672YGL2">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003822.1">
        <property type="protein sequence ID" value="ENSSORP00005003714.1"/>
        <property type="gene ID" value="ENSSORG00005002227.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YGL2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048667">
        <property type="term" value="P:cell morphogenesis involved in neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Delta/notch like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000057">
        <property type="entry name" value="protocadherin Fat 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000745">
        <property type="entry name" value="Sushi, nidogen and EGF like domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000633">
        <property type="entry name" value="Sushi, nidogen and EGF like domains 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000213">
        <property type="entry name" value="sushi, nidogen and EGF-like domain-containing protein 1"/>
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    </dbReference>
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        <property type="entry name" value="Sushi, nidogen and EGF-like domain-containing protein 1"/>
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        <property type="entry name" value="Sushi, nidogen and EGF-like domains 1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000309">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0768">Sushi</keyword>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025414694" evidence="14">
        <location>
            <begin position="32"/>
            <end position="1322"/>
        </location>
    </feature>
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        <location>
            <begin position="110"/>
            <end position="265"/>
        </location>
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        </location>
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        <location>
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            <end position="1153"/>
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    <sequence length="1322" mass="144970" checksum="2404C17F8C292721" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <sequence length="410" mass="45730" checksum="ECA4BC966C8F0C3B" modified="2020-06-17" version="1">VTVDGSSKRILQKEKMKNQLMVKQDTKQRERLSRSMDVISLISDDEDDVIRACSKTPFCLYLYTGVKLGPGETITVVLIGYFDQRSGVSVVRLLDTLQKDVRFQTSEDQRLLMELLVKLQLPLSNLVVFYCDAPHPEAFVSQILALNPTVVSLCRLPGLAERACRAGLLASFSYVGDLVRDLHRHCSTTNSVNGTLKELFADSGSYNPLCPESGDVSFIIHIVQKLVAIWWDVMEYFKSLSPERSVECLRSQLMDPKVHLDFLFLSHALEPLRALQNLQGGGSCDTAVELQLTAMLVNTYAASLLRPSAAERFLRRPDLLLLYSEKDLLPSTEVNIGFQAANFLLAAPLTDVSEQQDFVNRVVAFYRAALHSLVGSIPKQLGPMVLKNIGTMLKHPTNITVRPQSVYLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHD6</accession>
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        </lineage>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="440" mass="49463" checksum="C93D73C4906134E7" modified="2020-06-17" version="1">ELKFLVSGKTDCPYYAKAELLADTLQRCLPDFRTHKIAILPDEWKEWLESTCQKNGWKHVQSPLIWRELVDQGGKGMFLGGFSDFLEHCQDYYNITSDMSTEMMLKVAEENLSAKMSLIVDEQQRLILPHLSSALSPTCLFLIPNLLSAEVFPNVSMISIHLLDLEGTKEDLQGLKMETEDLAAPLLYQARDGENEEEKKNMIKSITECYKEYGRLINERAKKVVKVMVSGDSFVNLKCSLLLENASSINCCCFVAAATQLENEARVVIAKKLNVTTSDVTDVIVWGNISGSFFIDLQRAKVYNYDGAIKGPAFFSQPVQQIIYDRKWLETEFQDLVRYQRAAVTSKTCRTAAMSAANGILTVLKAWSGTGRTDQVLSVGVYATFVGKYDLPEDVILSVPVTFTAGKWSVVPEVNIEDKLRDKLRLSATELRQVCQVTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXC7</accession>
    <name>A0A672YXC7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 16">Mitochondrial import inner membrane translocase subunit TIM44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">timm44</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19. The complex interacts with the TIMM23 component of the TIM23 complex. Interacts with SLC25A4/ANT1 and SLC25A5/ANT2; leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the Tim44 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YXC7"/>
    <dbReference type="Ensembl" id="ENSSORT00005009574.1">
        <property type="protein sequence ID" value="ENSSORP00005009268.1"/>
        <property type="gene ID" value="ENSSORG00005005068.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YXC7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.240:FF:000001">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017303">
        <property type="entry name" value="Tim44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00984">
        <property type="entry name" value="3a0801s03tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037871">
        <property type="entry name" value="TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 16" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10 16" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim44-like" evidence="19">
        <location>
            <begin position="289"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR037871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="445" mass="50644" checksum="91BFDF0D88C64564" modified="2020-06-17" version="1">MICLKCSFPSYLLLHNRHNVFRICGSLTSPFQVQIKEVVRYLSGERGGGRKGFLGEFLDNIKQELNKNKEMKENIKKFREEAKKLEESDALKQARRKYKTIESETVKTSEVLKKKLGNLSETVKEGLEEVTRTEIGKKIKEGVEEAAKTAKSSAETVSKSGEMLGKTSAFKAISQGMESVKKEIGDLGHTGPYRPPARLRKRTEFSSKASGDGSKVFEANEEAMGVVLHKDSKWYQQWKDFKDNNMVFNRFFEMKMKYDESDNAFIRASRAVTDKMTDLIGGLFSKTEMSEVLTEILKVDPSFDKDSFLKQCERDIIPNILEAMIRGELEVLKDWCYEATYSQLAHPIQQAKAMGLQFHSKILDIDNIDLAMGKMMDQGPVLIITFQAQLVMVIRNTKGDVIEGDPDKVLRMMYVWALCRDQEELNPHAAWRLLDISASSTEQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YY78</accession>
    <name>A0A672YY78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YY78"/>
    <dbReference type="Ensembl" id="ENSSORT00005009839.1">
        <property type="protein sequence ID" value="ENSSORP00005009520.1"/>
        <property type="gene ID" value="ENSSORG00005005212.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YY78"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="13">
        <location>
            <begin position="253"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Tf2-1-like SH3-like" evidence="14">
        <location>
            <begin position="368"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="109"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="436" mass="50007" checksum="AEC12B2A0682031D" modified="2020-06-17" version="1">MTEAVAVLQEELSHCRNSINTLSAEITTQPAKISLGHVSVQTQYQTLPETLTSKPGSPESAALPPNKKPPIHPNQPVHNRPPPQVFCWRCKGNHAPVLCPQWIFNQVNRPSSHRPPQPQTAKPFQTQEGHATLGSLSHSGYPEAKTSPPKLALYSAIPPSMDSPPPCLDPQVQPATDADVLQFQQDTHKNLHIPNADIWKAQQEDSAIKDLYRKIMETGEVIENPTTKFTILEDKVYRVVQLPHKTHYQIYLPTTLRQQFLHHYHIEPLSGHLGRYKTFKRLQALVYWPKMSLDVREYVRCCQVCQRQKSETRKPPAKLHQHAIHAPPEMLGVAQFQKLVKGNLEKARKRQKQNYDKGRRDGNFTDNDRVWLHTHPYSKAEQLFSEKLAPRWKGPYRVTRRFEPLNLEVILEDTGQDLHVVNVAQLEPCYPPRQNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYJ2</accession>
    <name>A0A672YYJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Insulin-like growth factor-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115417772</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Binds equally well IGF1 and IGF2. Interacts with integrin ITGA5:ITGB1. Interacts with VHL; this interaction inhibits HIF1A degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987717.1">
        <property type="nucleotide sequence ID" value="XM_030131857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YYJ2"/>
    <dbReference type="Ensembl" id="ENSSORT00005009898.1">
        <property type="protein sequence ID" value="ENSSORP00005009579.1"/>
        <property type="gene ID" value="ENSSORG00005005232.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417772"/>
    <dbReference type="CTD" id="317638"/>
    <dbReference type="InParanoid" id="A0A672YYJ2"/>
    <dbReference type="OrthoDB" id="9926277at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031994">
        <property type="term" value="F:insulin-like growth factor I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031995">
        <property type="term" value="F:insulin-like growth factor II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048640">
        <property type="term" value="P:negative regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043567">
        <property type="term" value="P:regulation of insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.20:FF:000001">
        <property type="entry name" value="Insulin-like growth factor binding protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000002">
        <property type="entry name" value="Insulin-like growth factor-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022322">
        <property type="entry name" value="IGFBP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022321">
        <property type="entry name" value="IGFBP_1-6_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017891">
        <property type="entry name" value="Insulin_GF-bd_Cys-rich_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551:SF6">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01976">
        <property type="entry name" value="IGFBPFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01977">
        <property type="entry name" value="IGFBPFAMILY1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00222">
        <property type="entry name" value="IGFBP_N_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="6" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025636675" description="Insulin-like growth factor-binding protein 1" evidence="13">
        <location>
            <begin position="31"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="15">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="14">
        <location>
            <begin position="181"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="107"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="260" mass="27786" checksum="FC4227E6AFC72BBD" modified="2020-06-17" version="1">MLAMDGLYVKHVVVAALCSVLATGSPVVGPEPIRCAPCTPEKLSQCPAVAPECAEVLREPGCGCCLACALKAGELCGIYTAPCGSGLRCTPRPGDPRPLHSLTRGQAVCTDSTEPESTPEPQTQDQGEPEPEMENAAIVSDPGSSHYLPGLNKPYDPRAAADAQESMKAKLIAIRKKLVEQGPCHVELQRALDKIAKSQQKLGDKLTRFYLPNCDKHGLYKPKQCESSLDGQRGRCWCVNSWNGKKILGSTDVSAESECP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYK2</accession>
    <name>A0A672YYK2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Lectin, mannose-binding 2-like b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lman2lb</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YYK2"/>
    <dbReference type="Ensembl" id="ENSSORT00005009908.1">
        <property type="protein sequence ID" value="ENSSORP00005009589.1"/>
        <property type="gene ID" value="ENSSORG00005005237.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYK2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000017">
        <property type="entry name" value="Vesicular integral-membrane protein VIP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF20">
        <property type="entry name" value="VIP36-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="13">
        <location>
            <begin position="55"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="355" mass="40510" checksum="A53BBDE3A9668B27" modified="2020-06-17" version="1">MVAAVTKSNPNRLRSLGFLCPMFRLKNLRQLTCLFVAVWLLLSPSLAQEQDFMEDFLKREYSLVKPYRGLGFSSSSQWDLMGTAMVTPDYVRLTPDLQSRQGAVWSRVPCYLRDWELKVHFKISGQGKKNFNGDGLALWLTKERMQNGPVFGNMNQFTGLGIFVDTYPNIDKSHDRAFPYISVMLGNGTLTYDHDRDGRTTELGGCTAIARNSIYDTFLLIRYVKNRLTVMTDVEGKQEWKDCADITGLRLPLGYFFGASSATGDLSDNHDIISMKLYQLSVERTTEEEEQEEVLLPRVDNMDQFQGKVEVEDEGTSGVHLFFTFLFSVLGLGVLAVVGLIVYGRWKENRRKRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0D1</accession>
    <name>A0A672Z0D1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">26S proteasome regulatory subunit RPN10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115428961</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S5A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z0D1"/>
    <dbReference type="FunCoup" id="A0A672Z0D1">
        <property type="interactions" value="1942"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010659.1">
        <property type="protein sequence ID" value="ENSSORP00005010325.1"/>
        <property type="gene ID" value="ENSSORG00005005582.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0D1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22297">
        <property type="entry name" value="PSMD4_RAZUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01452">
        <property type="entry name" value="VWA_26S_proteasome_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000005">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.300.40:FF:000002">
        <property type="entry name" value="Proteasome 26S subunit, non-ATPase 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.300.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027040">
        <property type="entry name" value="PSMD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049590">
        <property type="entry name" value="PSMD4_RAZUL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223:SF13">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VWFA" evidence="7">
        <location>
            <begin position="24"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="239"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="351"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="387" mass="41717" checksum="04BBEDE9630582F0" modified="2020-06-17" version="1">CVCLGFYKSDQSRLVLLSALSNSLVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITMANNCEVLTTLTPDSGRILSKLHAVQPRGKISFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFINTLNGKEGTGSHLVTVPPGPSLADALLSSPILAGEGGSMMGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGMPTPSADESEEALLKMSVSQPETGAAVLPDFSSMTEEEQIAYAMQMSLAGGEYGEMDTGAAMDTAESAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQTGNKPDGKKDEEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1C6</accession>
    <name>A0A672Z1C6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Wnt inhibitory factor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">seraf</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z1C6"/>
    <dbReference type="Ensembl" id="ENSSORT00005010851.1">
        <property type="protein sequence ID" value="ENSSORP00005010514.1"/>
        <property type="gene ID" value="ENSSORG00005005681.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1C6"/>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070697">
        <property type="term" value="F:activin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038100">
        <property type="term" value="F:nodal binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001568">
        <property type="term" value="P:blood vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038092">
        <property type="term" value="P:nodal signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000020">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050969">
        <property type="entry name" value="Dev_Signal_Modulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949">
        <property type="entry name" value="EGF-LIKE-DOMAIN, MULTIPLE 7, 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949:SF46">
        <property type="entry name" value="SCHWANN CELL-SPECIFIC EGF-LIKE REPEAT AUTOCRINE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025592526" evidence="5">
        <location>
            <begin position="27"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="211" mass="22061" checksum="BCD0E612A6A03BBE" modified="2020-06-17" version="1">MDVPGLGSAFFLLTSVILALSGPCAARSDAPRGVALGFVFDSKAKCDPPCEHAGICIRNNTCFCSRGYEGETCQYATCYPKCKNGGVCLRPGKCRCPPGYGGRYCHKVTCDGGCWNGGECTAVNGVAMCICTSSWTGSKCQEAICPQGCRNGGICVAPGICSCPDGWLGGACHTAVCSQPCLNGGKCVSPEKCRCRPPFSGPRCEERKKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1K7</accession>
    <name>A0A672Z1K7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Monocarboxylate transporter 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">slc16a3a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSSORT00005011436.1">
        <property type="protein sequence ID" value="ENSSORP00005011061.1"/>
        <property type="gene ID" value="ENSSORG00005005965.1"/>
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    <dbReference type="InParanoid" id="A0A672Z1K7"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015650">
        <property type="term" value="F:lactate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS_MCT3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000077">
        <property type="entry name" value="Proton-coupled monocarboxylate transporter 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004743">
        <property type="entry name" value="MCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00892">
        <property type="entry name" value="2A0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF27">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="147"/>
            <end position="166"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="21"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="445"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="460"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="508"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="522" mass="56760" checksum="AA6D4204B9963EC0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4D6</accession>
    <name>A0A672Z4D6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Collagen, type XI, alpha 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">col11a2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005011957.1">
        <property type="protein sequence ID" value="ENSSORP00005011573.1"/>
        <property type="gene ID" value="ENSSORG00005004250.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z4D6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005594">
        <property type="term" value="C:collagen type IX trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Collagen XI alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000885">
        <property type="entry name" value="Fib_collagen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF372">
        <property type="entry name" value="COLLAGEN ALPHA-1(XVI) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01410">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00038">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51461">
        <property type="entry name" value="NC1_FIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025612213" evidence="9">
        <location>
            <begin position="24"/>
            <end position="1632"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrillar collagen NC1" evidence="10">
        <location>
            <begin position="1402"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="409"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="538"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1103"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1179"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="679"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="709"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="748"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="925"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1011"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1117"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1194"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1207"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1268"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1302"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1632" mass="167205" checksum="5B89D7DEE6DC525E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7K5</accession>
    <name>A0A672Z7K5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 710</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">znf366</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z7K5"/>
    <dbReference type="Ensembl" id="ENSSORT00005013246.1">
        <property type="protein sequence ID" value="ENSSORP00005012844.1"/>
        <property type="gene ID" value="ENSSORG00005006710.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z7K5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000161">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000182">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000186">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000191">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000203">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001574">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001721">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000451">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000502">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="450"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="478"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="506"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="534"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="73"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="588"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="645"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="680"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="704"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="721"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="746"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="780" mass="90226" checksum="76E4C03DE0E63D05" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8W9</accession>
    <name>A0A672Z8W9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Meiosis-specific nuclear structural protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">mns1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. May play a role in the control of meiotic division and germ cell differentiation through regulation of pairing and recombination during meiosis. Required for sperm flagella assembly. May play a role in the assembly and function of the outer dynein arm-docking complex (ODA-DC). ODA-DC mediates outer dynein arms (ODA) binding onto the axonemal doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNS1 family.</text>
    </comment>
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    <dbReference type="FunCoup" id="A0A672Z8W9">
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    <dbReference type="Ensembl" id="ENSSORT00005014232.1">
        <property type="protein sequence ID" value="ENSSORP00005013816.1"/>
        <property type="gene ID" value="ENSSORG00005007091.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z8W9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026504">
        <property type="entry name" value="MNS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19265">
        <property type="entry name" value="MEIOSIS-SPECIFIC NUCLEAR STRUCTURAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19265:SF0">
        <property type="entry name" value="MEIOSIS-SPECIFIC NUCLEAR STRUCTURAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="16">
        <location>
            <begin position="103"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="16">
        <location>
            <begin position="204"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="212"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="379"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="465" mass="58078" checksum="B3E687613CCA3BC4" modified="2020-06-17" version="1">MMYHHQRLMSQRQEQRREEATRLVDRDRQLQASLRDEEGFERRRYLRQVQEELKERQMESALLKAEEERLNRQKQLEQEERVAKHLARINYEKQREEKMRQYIKENSLELRELEAKLKSAYLNKERAAQIAEQEAMRFETMREEADYTRRMKEEHERSATEQQKLEQRRQEELLQHQRELEQQMMDRELLILTLKLPHIYPTLATQQHIEEFKRQQAEWKRMEQERMEAENRRIMEFANRQQQKEDSRRAQMRVREEEKQQLHKMLSEKIEKERQQREEMERVREELYLEEQEEANRQREIEEMEKKIKQRLMMQQTCQQQMAFKEMQRQAEKEQEEAFRRMMMAKFAEDDRIEQMNAQKRRMKQLEHKREVEKLIEDRRRQHEADKELEAKERALEEEREALRRQIIEEERQRLLKRHATKLLGYLPKGLLRENDLDHFDEDFRKNFQSRQTDFFSEDGWEGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCZ8</accession>
    <name>A0A672ZCZ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZCZ8"/>
    <dbReference type="Ensembl" id="ENSSORT00005014754.1">
        <property type="protein sequence ID" value="ENSSORP00005014332.1"/>
        <property type="gene ID" value="ENSSORG00005007338.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZCZ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029625">
        <property type="entry name" value="FAM169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22442">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22442:SF4">
        <property type="entry name" value="PROTEIN FAM169BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="202" mass="23155" checksum="0A513E489430B667" modified="2020-06-17" version="1">MFPVDLLSVDHTDLTSAAQNYLSTLESRAGAEEWFRSSATSKVRVTPQNIRHLQLFDRPKCVLLALHQSDDPTQVVAVYLPDRWWAVDDVLRTSNASRSGFQSVQSVMERVVVFLLSQVVDRPLQEEVHFYPHPPTETCKLLWREGEAVGFYSVKHKGSLCDGWSSRCYQLTVLDTLLVRRRCRRSGLGLHILDHFCNTFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZD27</accession>
    <name>A0A672ZD27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">14-3-3 protein epsilon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115433097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030010173.1">
        <property type="nucleotide sequence ID" value="XM_030154313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZD27"/>
    <dbReference type="Ensembl" id="ENSSORT00005015731.1">
        <property type="protein sequence ID" value="ENSSORP00005015241.1"/>
        <property type="gene ID" value="ENSSORG00005007738.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015732.1">
        <property type="protein sequence ID" value="ENSSORP00005015242.1"/>
        <property type="gene ID" value="ENSSORG00005007738.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433097"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="7">
        <location>
            <begin position="5"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="4">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="4">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="256" mass="29227" checksum="5C7F9AEC201263A5" modified="2020-06-17" version="1">MMADRENLVYQAKLAEQAERYDEMVESMKRVASLDLELTVEERNLLSVAYKNVIGARRASWRIISSLEQKEESKGSEDKLKMIREYRKTVEKELKSICNDILDVLDKHLILSANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDNAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDVQGDGEEQNKEALQDAEDEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZF71</accession>
    <name>A0A672ZF71_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Epidermal growth factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">egf</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZF71"/>
    <dbReference type="Ensembl" id="ENSSORT00005016129.1">
        <property type="protein sequence ID" value="ENSSORP00005015635.1"/>
        <property type="gene ID" value="ENSSORG00005007938.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZF71"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000219">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000036">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF13">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="874"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="28"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="70"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="460"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="538"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="700"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="747"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="813"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="825"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="844"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="898" mass="99585" checksum="F863929722B79B3C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI49</accession>
    <name>A0A672ZI49_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vitamin K-dependent gamma-carboxylase</fullName>
            <ecNumber evidence="2">4.1.1.90</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Gamma-glutamyl carboxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Peptidyl-glutamate 4-carboxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Vitamin K gamma glutamyl carboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ggcx</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4-carboxy-L-glutamyl-[protein] + 2,3-epoxyphylloquinone + H2O + H(+) = phylloquinol + L-glutamyl-[protein] + CO2 + O2</text>
            <dbReference type="Rhea" id="RHEA:45140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:11094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15759"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28433"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:84990"/>
            <dbReference type="EC" id="4.1.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:45142"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.1.1.90" evidence="2"/>
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        <property type="nucleotide sequence ID" value="XM_030149233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZI49"/>
    <dbReference type="Ensembl" id="ENSSORT00005017313.1">
        <property type="protein sequence ID" value="ENSSORP00005016800.1"/>
        <property type="gene ID" value="ENSSORG00005008212.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429620"/>
    <dbReference type="CTD" id="2677"/>
    <dbReference type="InParanoid" id="A0A672ZI49"/>
    <dbReference type="OrthoDB" id="206689at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008488">
        <property type="term" value="F:gamma-glutamyl carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019842">
        <property type="term" value="F:vitamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011020">
        <property type="entry name" value="HTTM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053934">
        <property type="entry name" value="HTTM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007782">
        <property type="entry name" value="VKG_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053935">
        <property type="entry name" value="VKGC_lumenal_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12639">
        <property type="entry name" value="VITAMIN K-DEPENDENT GAMMA-CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12639:SF6">
        <property type="entry name" value="VITAMIN K-DEPENDENT GAMMA-CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05090">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22777">
        <property type="entry name" value="VKGC_lumenal_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00752">
        <property type="entry name" value="HTTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="HTTM-like" evidence="17">
        <location>
            <begin position="60"/>
            <end position="319"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="341"/>
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        </location>
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        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
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        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012248"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="765" mass="88605" checksum="DE216AA10FDB4B38" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZIX8</accession>
    <name>A0A672ZIX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SS18 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ss18l2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SS18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029980941.1">
        <property type="nucleotide sequence ID" value="XM_030125081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZIX8"/>
    <dbReference type="FunCoup" id="A0A672ZIX8">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017666.1">
        <property type="protein sequence ID" value="ENSSORP00005017151.1"/>
        <property type="gene ID" value="ENSSORG00005008606.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412516"/>
    <dbReference type="CTD" id="51188"/>
    <dbReference type="InParanoid" id="A0A672ZIX8"/>
    <dbReference type="OrthoDB" id="10265171at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007726">
        <property type="entry name" value="SS18_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05030">
        <property type="entry name" value="SSXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SS18 N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="97" mass="10887" checksum="583E5DA1848F30DB" modified="2020-06-17" version="1">MSIVFVPKKLRGKAKINQETIQRLLDENDQLIRCITEYMQKGRAVECVQYQQILHRNIVYLATIADASPDIMAPSSKPTSDESSASAPTVNGHKETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKK1</accession>
    <name>A0A672ZKK1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115431762</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030008268.1">
        <property type="nucleotide sequence ID" value="XM_030152408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZKK1"/>
    <dbReference type="FunCoup" id="A0A672ZKK1">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018221.1">
        <property type="protein sequence ID" value="ENSSORP00005017701.1"/>
        <property type="gene ID" value="ENSSORG00005008816.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431762"/>
    <dbReference type="InParanoid" id="A0A672ZKK1"/>
    <dbReference type="OrthoDB" id="6147321at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="40"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="315" mass="36366" checksum="351244AC3DD76CE6" modified="2020-06-17" version="1">MIINSTQVSYFILSAYFDTGMLKYLYFTIILCLYVLIILSNVFLIVLICVNRSLHEPMYLFLCSLFVNQLYGSTGLFPFILLQILKDVHTVSFSLCVLQIYCLYSYVQVEIINLTVMSYDRYLSICFPLQYHTRMTQNKIAVLIAVVWVYPLLTVAVRRYLTVSLQLCGNIIDKIYCNNYSIVKLSCSDTTDNNIYGLINAFIFICVPAMLILYSYMRILKVCFSGSKQTRHKAVSTCTPHLASLINFTLGCCFEIVQSRFNMNHVPNMLRIFLSVYFVILQPVFNPMVYGMKMSKIRVKCNHLLCGKRHLDSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZM75</accession>
    <name>A0A672ZM75_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">T-complex protein 11-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115419071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989550.1">
        <property type="nucleotide sequence ID" value="XM_030133690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZM75"/>
    <dbReference type="Ensembl" id="ENSSORT00005018061.1">
        <property type="protein sequence ID" value="ENSSORP00005017542.1"/>
        <property type="gene ID" value="ENSSORG00005008761.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419071"/>
    <dbReference type="InParanoid" id="A0A672ZM75"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902490">
        <property type="term" value="P:regulation of sperm capacitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF14">
        <property type="entry name" value="T-COMPLEX PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="483" mass="53555" checksum="25389EE062CA7F07" modified="2020-06-17" version="1">MPNERDEASDESPNDLPCLEKLHSPTGSPPTASLSSLMELENSVSNLSLAHEIVVNRDFCFKPRSPPTDSLEGRVTEIVHRAFWDSLQEQLSSDPPEFSHAIILLQEVKTLLQSLLLPGHVRLRSQLDEVLDMELIQQEVNHGALDLHRLAGYIINTMGSLCAPVRDPEIRALRDLRDPVELFREIFRVLGLMKIDMVNFTVQTLRPHLMQQAVQYERAKFQQILDQHPASLDNTSAWLQAAASEEASAVSAGSEAPDSRSPVSATAVLNRAYIRLLHWSPQEQKYPETVLMDRARLDPLGRRLQMLVLEASVLLLTSAQCGGTVFSLQGFVGKLRQSITALLEGSHTREDDLKGALLGLGEKVLQQVNEALSRQEGAALPQENQDLLRGQITNLWKHNNPVRCLIGERVQGFLQAMLQGGPSKRSPDLPATLRLVGAELAELGTAFGQIVHFNRAVFGPFYAPILRKLLFPPGEAETGEDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZRJ9</accession>
    <name>A0A672ZRJ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">umad1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030003471.1">
        <property type="nucleotide sequence ID" value="XM_030147611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZRJ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005019557.1">
        <property type="protein sequence ID" value="ENSSORP00005019002.1"/>
        <property type="gene ID" value="ENSSORG00005009362.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428517"/>
    <dbReference type="CTD" id="729852"/>
    <dbReference type="InParanoid" id="A0A672ZRJ9"/>
    <dbReference type="OrthoDB" id="9872568at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053292">
        <property type="entry name" value="UBAP1-MVB12_assoc_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023340">
        <property type="entry name" value="UMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36291">
        <property type="entry name" value="UBAP1-MVB12-ASSOCIATED (UMA)-DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36291:SF1">
        <property type="entry name" value="UBAP1-MVB12-ASSOCIATED (UMA)-DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51497">
        <property type="entry name" value="UMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UMA" evidence="2">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="148" mass="15821" checksum="A5D4262E002EF640" modified="2020-06-17" version="1">MLSFLGLGRKDSKKSPTEKEADGGFVIVGETVEEQRQKMQSMNIAHPSTNVIVQPSKSSYKAPAPPTPFRPPTASSTTGPTVGPPAADGAPALPDLLGDVPFTLAPHVLAMQAGFPMIPDVLLSRDINYNLASFQYDFTLENSVLHNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZS66</accession>
    <name>A0A672ZS66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhombotin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">LIM domain only protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">lmo2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZS66"/>
    <dbReference type="FunCoup" id="A0A672ZS66">
        <property type="interactions" value="611"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019709.1">
        <property type="protein sequence ID" value="ENSSORP00005019153.1"/>
        <property type="gene ID" value="ENSSORG00005009413.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZS66"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09384">
        <property type="entry name" value="LIM1_LMO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09385">
        <property type="entry name" value="LIM2_LMO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000016">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF3">
        <property type="entry name" value="RHOMBOTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="36"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="100"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="168" mass="19547" checksum="C795D527FEF63E3F" modified="2020-06-17" version="1">YSEIYETKSLCSKIPSGFSFILEPVDEVLQMPPSLLTCGGCQQSIGDRFFLKAIEQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCEKRIRAFEMTMRVRDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIFEWTKLNSNNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTJ0</accession>
    <name>A0A672ZTJ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zgc:123107</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZTJ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005021202.1">
        <property type="protein sequence ID" value="ENSSORP00005020625.1"/>
        <property type="gene ID" value="ENSSORG00005010027.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZTJ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF86">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DR ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="119" mass="13259" checksum="4E4411489023A403" modified="2020-06-17" version="1">MKQTRLKALYSKNNHAPSAVLIYPKEDVLLDVQNTLVCHVSGFYPAPVQVQWTHNERNVSGAATGNVPYPSKDGTFSQISRLEFTPKQGDIYSCTVQHPALSTPLTRIWGKDQETLTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTT4</accession>
    <name>A0A672ZTT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="14">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">snrpe</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029991365.1">
        <property type="nucleotide sequence ID" value="XM_030135505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZTT4"/>
    <dbReference type="FunCoup" id="A0A672ZTT4">
        <property type="interactions" value="1505"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021150.1">
        <property type="protein sequence ID" value="ENSSORP00005020576.1"/>
        <property type="gene ID" value="ENSSORG00005010008.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420237"/>
    <dbReference type="CTD" id="6635"/>
    <dbReference type="InParanoid" id="A0A672ZTT4"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000059">
        <property type="entry name" value="Small nuclear ribonucleoprotein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="15">
        <location>
            <begin position="18"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="92" mass="10830" checksum="50889E2E24CE5827" modified="2020-06-17" version="1">MAYRGQGQKVQKVMVQPINLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEVHMKTKNRKPLGRIMLKGDNITLLQSVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVJ4</accession>
    <name>A0A672ZVJ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-hydroxytryptamine receptor 1D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Serotonin receptor 1D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">htr1d</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZVJ4"/>
    <dbReference type="Ensembl" id="ENSSORT00005020839.1">
        <property type="protein sequence ID" value="ENSSORP00005020268.1"/>
        <property type="gene ID" value="ENSSORG00005009887.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZVJ4"/>
    <dbReference type="OrthoDB" id="5956310at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050795">
        <property type="term" value="P:regulation of behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040012">
        <property type="term" value="P:regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006939">
        <property type="term" value="P:smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15333">
        <property type="entry name" value="7tmA_5-HT1B_1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000505">
        <property type="entry name" value="5HT1D_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF196">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00514">
        <property type="entry name" value="5HT1DRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="53"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="385" mass="42491" checksum="2D4D39ACFEC5CE6A" modified="2020-06-17" version="1">MELDNSSLDYFISNTSEIPDTVTDQPWSEATLLGFQISLSALLAVVTLATVLSNAFVIATIFLTRKLHTPANFLIGSLAVTDLLVSILVMPISIVYTVSKTWSLGQIVCDIWLSSDITFCTASILHLCVIALDRYWAITDALEYSKRRTMRRAGIMVGVVWVISISISMPPLFWRQAKAHEELTECMVNTDQISYTLYSTFGAFYVPTVLLIILYGRIYVAARSRIFKTPSSSGKRFTTAQLIQTSAGSSLCSLNSASNQEAHLHSGTGTGGGGGGGGSPLFLNSVKVKLADSVLERKRLCAARERKATKTLGIILGAFIICWLPFFVGTLVMAICKDCWFDPVLFDIFTWLGYLNSLINPVIYTAFNDEFKQAFQKLMKLRRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZW11</accession>
    <name>A0A672ZW11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pcdh18a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZW11"/>
    <dbReference type="FunCoup" id="A0A672ZW11">
        <property type="interactions" value="732"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021927.1">
        <property type="protein sequence ID" value="ENSSORP00005021286.1"/>
        <property type="gene ID" value="ENSSORG00005010414.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZW11"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000003">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
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        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
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        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF9">
        <property type="entry name" value="PROTOCADHERIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
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        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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    <feature type="chain" id="PRO_5025579243" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="26"/>
            <end position="1191"/>
        </location>
    </feature>
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        <location>
            <begin position="694"/>
            <end position="718"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
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            <begin position="136"/>
            <end position="244"/>
        </location>
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            <begin position="245"/>
            <end position="352"/>
        </location>
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            <begin position="368"/>
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        </location>
    </feature>
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        <location>
            <begin position="465"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="595"/>
            <end position="685"/>
        </location>
    </feature>
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        <location>
            <begin position="772"/>
            <end position="794"/>
        </location>
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            <begin position="865"/>
            <end position="885"/>
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        <location>
            <begin position="942"/>
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        </location>
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            <begin position="1031"/>
            <end position="1081"/>
        </location>
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        <location>
            <begin position="1102"/>
            <end position="1140"/>
        </location>
    </feature>
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        <location>
            <begin position="1152"/>
            <end position="1175"/>
        </location>
    </feature>
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        <location>
            <begin position="865"/>
            <end position="874"/>
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        <location>
            <begin position="1037"/>
            <end position="1068"/>
        </location>
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        <location>
            <begin position="1102"/>
            <end position="1130"/>
        </location>
    </feature>
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        <location>
            <begin position="1162"/>
            <end position="1175"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1191" mass="130544" checksum="F060C75E730D7835" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZ57</accession>
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        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="KAZRIN ISOFORM X1"/>
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    </dbReference>
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            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="326" mass="36644" checksum="E2E7AB760A8C59D1" modified="2020-06-17" version="1">MSLWRAGAVQAWLEVVMGMPMYLRACSENIKSGKVLLGLTDEDLELGLGITNPIHRRKLRLAIEDYRRAEGDQGLSKASDMDHHWVSTSWLSDVGLPQYSQTFQTHLVDGRVLNSLSRRDLERLLNISDQFHQTSLLLAIQLLQMLSFDKEALQARRAKCEHQDRDPIVWTCHRVMKWIRDIDLKEFAENLQGKGIHGAVMALDPSFDTDAMAKALGIPNNKHMLHRHLYEEMKTLAVPPSNSEQSSEPIGSSSPVAVTRLAEERMSVRRSGKSPLRLRANSHSAERGLGFHGSCGSLPREARVQAVPRVKGSPMHTYKSVEITNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZG7</accession>
    <name>A0A672ZZG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase RNF182</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RING finger protein 182</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase RNF182</fullName>
        </alternativeName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Interacts with ATP6V0C.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZZG7"/>
    <dbReference type="Ensembl" id="ENSSORT00005023043.1">
        <property type="protein sequence ID" value="ENSSORP00005022384.1"/>
        <property type="gene ID" value="ENSSORG00005010896.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZG7"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042285">
        <property type="entry name" value="RNF182"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46675">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46675:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="20"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011482"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014050"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="231" mass="25596" checksum="D7B9045220653522" modified="2020-06-17" version="1">TLYYPKENLFTPKNAEELECKICYQRYNVHNRKPKILDCLHRVCARCLIKILDIADGGGSISCPFCRHHTEITEYEVSALPDDANIMSHLACRDKSSGSDHNREVVLTPKSFSSSSPSHDSSNCLVITIMEVQRDSQHSPSRNGSSDIYAEQSLDSVSLGSNGPAEQDTLSKFCNHVPRILVWLLGFLYFGSLPLGIYLCVSLVPSSLTVCLVYGFCQCLCQGMCDCSTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZM6</accession>
    <name>A0A672ZZM6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Apolipoprotein M</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with LRP2; LRP2 mediates APOM renal uptake and subsequent lysosomal degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Lipocalin family. Highly divergent.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZZM6"/>
    <dbReference type="Ensembl" id="ENSSORT00005022591.1">
        <property type="protein sequence ID" value="ENSSORP00005021937.1"/>
        <property type="gene ID" value="ENSSORG00005010709.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZM6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034362">
        <property type="term" value="C:low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034380">
        <property type="term" value="P:high-density lipoprotein particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034384">
        <property type="term" value="P:high-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
        <property type="term" value="P:high-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022734">
        <property type="entry name" value="ApoM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32028">
        <property type="entry name" value="APOLIPOPROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32028:SF1">
        <property type="entry name" value="APOLIPOPROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11032">
        <property type="entry name" value="ApoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0345">HDL</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="136" mass="15606" checksum="290EBD59A9AC23C2" modified="2020-06-17" version="1">IQQFKEMDSVLFTMEDTGNATLLLTGHMRIGDHCINQTWTYSMHLGRDDLELKGRPQRRNLLWSGKWANCSDCIIFQEIEPPLNETGTEDSLNRLMLYSRKSDLDADSVTAFQKNAACNNMTAFVRLPQDKGLCFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZQ7</accession>
    <name>A0A672ZZQ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115426074</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KCMF1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_029999881.1">
        <property type="nucleotide sequence ID" value="XM_030144021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZZQ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005022621.1">
        <property type="protein sequence ID" value="ENSSORP00005021967.1"/>
        <property type="gene ID" value="ENSSORG00005010731.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426074"/>
    <dbReference type="InParanoid" id="A0A672ZZQ7"/>
    <dbReference type="OrthoDB" id="7873042at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02338">
        <property type="entry name" value="ZZ_PCMF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="10">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="154"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="242"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="420" mass="45642" checksum="038047F802CA4D0E" modified="2020-06-17" version="1">MSRHEGVSCDACLKGNFRGRRYKCLICYDYDLCASCYESGATTTRHTTEHPMQCILTRVDFDLYYGGEAFSVEQPQAFTCPYCGRMGYTEISLQEHVAAEHTETSTEVICPICAALPGGDPNHVTDDFAAHLTLEHRAPRDLDESSGVRHVRRMFHPGRGLGGPRARRSNMHFTSSTTGGLSTSQSSSQSSSYSREAMDPIAELLSQLSGVRRSAGGQLSSGPSASQLQQLQMQLQLERQQAQAARQQLETARNATRGGGRANAILNTNSAVANPNPSANHNTNPSPNPGPPEPSTQHTAHSSQFLLSRLSEPRLSEAERQACEAQWADRSLFVTELLLSTLLPDEDASASEDDDEGDADNCHGPLRNFADFQAMGCVEVMTLDVALENLNLKEMTPTSKTTKTTTKTAPAKKEPPAPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1C2</accession>
    <name>A0A673A1C2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized LOC115419375</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fam83e</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM83 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A1C2"/>
    <dbReference type="FunCoup" id="A0A673A1C2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023197.1">
        <property type="protein sequence ID" value="ENSSORP00005022532.1"/>
        <property type="gene ID" value="ENSSORG00005010960.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A1C2"/>
    <dbReference type="OrthoDB" id="8943940at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012461">
        <property type="entry name" value="SACK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF29">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07894">
        <property type="entry name" value="SACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffolding anchor of CK1" evidence="3">
        <location>
            <begin position="17"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="400"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="635" mass="72008" checksum="3D5EB84FEE830F13" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3V0</accession>
    <name>A0A673A3V0_9TELE</name>
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        <submittedName>
            <fullName evidence="7">Signal-transducing adaptor protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115418028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029988176.1">
        <property type="nucleotide sequence ID" value="XM_030132316.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A673A3V0"/>
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        <property type="protein sequence ID" value="ENSSORP00005023924.1"/>
        <property type="gene ID" value="ENSSORG00005011548.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418028"/>
    <dbReference type="InParanoid" id="A0A673A3V0"/>
    <dbReference type="OrthoDB" id="6086001at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13268">
        <property type="entry name" value="PH_Brdg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039111">
        <property type="entry name" value="STAP1/STAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16186:SF11">
        <property type="entry name" value="SIGNAL-TRANSDUCING ADAPTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16186">
        <property type="entry name" value="SIGNAL-TRANSDUCING ADAPTOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0727">SH2 domain</keyword>
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        <location>
            <begin position="14"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="184"/>
            <end position="279"/>
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            <begin position="137"/>
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        <location>
            <begin position="142"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
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            <begin position="411"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="423" mass="46691" checksum="AC79978F40B2B444" modified="2020-06-17" version="1">MAKRTGRQKTQLPTCYYEGYLEKRSFKDKTSRKLWTCLCGNTLFFFNDKRDSDYVEKVELSGLISITDDCSVDRNLDAARLNLQTKEGNIKFTAPTAEARELWKGFIHSVAQLSVPSSLNLLPGQIHMLKEAVDKEKERLYSTTPPAVPATPSTSASTSTSTSTSTSPSTSTSPYVSLLADMPVCYHKVTRLEAELLLDREVARGNLLLRPGRNGNSYAVTTRQDLNGPVFKHYRVTRRHEGGFLIDVENPVPCTTLHDVVNYLVEQTEGVLIPLVFEEAYEKSISFIQSDSENGEKSVHQAQTNPVPPSVPPKPASRRIPTPEPVPAPAEVTEECVYLNDMPPAEEEKQAEGNSPVPPPLEKKPPKKAIMPPVAPLRKTSTCTPPPSTTSSTSSRVRANTDPMGQAISELKLKLEKKTKIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4J1</accession>
    <name>A0A673A4J1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LIM and SH3 domain protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">lasp1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A4J1"/>
    <dbReference type="FunCoup" id="A0A673A4J1">
        <property type="interactions" value="2159"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024230.1">
        <property type="protein sequence ID" value="ENSSORP00005023547.1"/>
        <property type="gene ID" value="ENSSORG00005011400.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4J1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09447">
        <property type="entry name" value="LIM_LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11934">
        <property type="entry name" value="SH3_Lasp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000087">
        <property type="entry name" value="LIM zinc-binding domain-containing Nebulette"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000007">
        <property type="entry name" value="nebulin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035630">
        <property type="entry name" value="Lasp1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051759">
        <property type="entry name" value="LIM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000900">
        <property type="entry name" value="Nebulin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218">
        <property type="entry name" value="LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218:SF2">
        <property type="entry name" value="LIM AND SH3 DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00880">
        <property type="entry name" value="Nebulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00227">
        <property type="entry name" value="NEBU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51216">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0440">LIM domain</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 19" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="11 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="21">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="178"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="237" mass="27339" checksum="C387847664DBEE06" modified="2020-06-17" version="1">MNPLCSRCHKVVYPVEKLNCLDKYWHKACFSCEVCKMTLNMKNYKGFEKRPYCNAHYPKTNFTAVVDTPENLRLKQQTEIQSQVRYKEDFEKNKGKGFSVVTDTPEMKRIKKTQDQVSNIKYHEEFEKRKMGGEFIYLCSRPLFVLSAAYQSSAATQNYHYDPTPEPVRQAAAAPPPSAGKRYRAVYDYSAADDDEVSFMDGDVIVDVQQIDEGWMYGRVERTGQQGMLPANYVEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A594</accession>
    <name>A0A673A594_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vitellogenin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A594"/>
    <dbReference type="Ensembl" id="ENSSORT00005024568.1">
        <property type="protein sequence ID" value="ENSSORP00005023868.1"/>
        <property type="gene ID" value="ENSSORG00005011510.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A594"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.80.10">
        <property type="entry name" value="Lipovitellin-phosvitin complex, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.50.20">
        <property type="entry name" value="Lipovitellin. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015817">
        <property type="entry name" value="Vitellinogen_open_b-sht_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050733">
        <property type="entry name" value="Vitellogenin/Apolipophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345:SF9">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Vitellogenin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="769"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="810"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="774"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="816"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="831"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="846"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="890"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="900"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="934"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="976" mass="108978" checksum="15FBD5BE805096AD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A623</accession>
    <name>A0A673A623_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">43 kDa receptor-associated protein of the synapse</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">43 kDa postsynaptic protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Acetylcholine receptor-associated 43 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">rapsn</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Postsynaptic protein required for clustering of nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Peripheral membrane protein</topology>
            <orientation evidence="18">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RAPsyn family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029991657.1">
        <property type="nucleotide sequence ID" value="XM_030135797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A623"/>
    <dbReference type="FunCoup" id="A0A673A623">
        <property type="interactions" value="706"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024818.1">
        <property type="protein sequence ID" value="ENSSORP00005024107.1"/>
        <property type="gene ID" value="ENSSORG00005011625.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420511"/>
    <dbReference type="CTD" id="5913"/>
    <dbReference type="InParanoid" id="A0A673A623"/>
    <dbReference type="OrthoDB" id="10040854at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033130">
        <property type="term" value="F:acetylcholine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900075">
        <property type="term" value="P:positive regulation of neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16478">
        <property type="entry name" value="RING-H2_Rapsyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000123">
        <property type="entry name" value="43 kDa receptor-associated protein of the synapse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000206">
        <property type="entry name" value="43 kDa receptor-associated protein of the synapse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001237">
        <property type="entry name" value="Postsynaptic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018293">
        <property type="entry name" value="Postsynaptic_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052480">
        <property type="entry name" value="RAPsyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019568">
        <property type="entry name" value="Rapsyn_myristoylation/link_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46574">
        <property type="entry name" value="43 KDA RECEPTOR-ASSOCIATED PROTEIN OF THE SYNAPSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46574:SF1">
        <property type="entry name" value="43 KDA RECEPTOR-ASSOCIATED PROTEIN OF THE SYNAPSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10579">
        <property type="entry name" value="Rapsyn_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00217">
        <property type="entry name" value="POSTSYNAPTIC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00405">
        <property type="entry name" value="43_KD_POSTSYNAPTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="10 23" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="TPR" evidence="23">
        <location>
            <begin position="123"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="363"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00077104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="412" mass="46970" checksum="DE9092BFF30C9FA0" modified="2020-06-17" version="1">MGQDQTKQQIEKGLRLYQSNQTEKALHVWTKVLEKTSDPGGKFRVLGCLITAHSEMGKYKDMLKYALDQIDTAREMEDPDYLTEGYLNLARSNEKLCDFQKTVSYCKTCLNMQGTTVSLQLNGQVCLSMGNAFLGLSVFQKALESYEKALRYAHNNDDKMLECRVCCSLGNIYVQLKDYEKALFFPCKAAELVNDYGKGWSLKYRAMSQYHMSVAYRKLERLPDAMECCEESMKIALQHGDRPLQALCLLNFADIHRCRRDVDKAFPRYESALGIMTEIGNRLGQIYVYLGVAKCWLLQKEFDKALDSLQRAQDLADAIGNKLCTLKVHCLCEGIYRSRGQQEELREQVVKFLQCVEELELYCGMCGESIGDRDQKLQALPCSHIFHLKCLETNGTKGCPKCFKSSMKPGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACM4</accession>
    <name>A0A673ACM4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Platelet-derived growth factor receptor alpha</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Alpha platelet-derived growth factor receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Alpha-type platelet-derived growth factor receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="39" type="primary">pdgfra</name>
    </gene>
    <organism evidence="39 40">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="39" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="39" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="39" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Interacts with homodimeric pdgfa, pdgfb and pdgfc, and with heterodimers formed by pdgfa and pdgfb. Monomer in the absence of bound ligand. Interaction with dimeric pdgfa, pdgfb and/or pdgfc leads to receptor dimerization, where both pdgfra homodimers and heterodimers with pdgfrb are observed.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="33">Membrane</location>
            <topology evidence="33">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="33">Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_029988615.1">
        <property type="nucleotide sequence ID" value="XM_030132755.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A673ACM4">
        <property type="interactions" value="1212"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027034.1">
        <property type="protein sequence ID" value="ENSSORP00005026262.1"/>
        <property type="gene ID" value="ENSSORG00005009806.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418330"/>
    <dbReference type="CTD" id="5156"/>
    <dbReference type="InParanoid" id="A0A673ACM4"/>
    <dbReference type="OrthoDB" id="9936425at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005018">
        <property type="term" value="F:platelet-derived growth factor alpha-receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048407">
        <property type="term" value="F:platelet-derived growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001667">
        <property type="term" value="P:ameboidal-type cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000720">
        <property type="entry name" value="Platelet-derived growth factor receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000025">
        <property type="entry name" value="Platelet-derived growth factor receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000140">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000223">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027290">
        <property type="entry name" value="PDGFRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001824">
        <property type="entry name" value="Tyr_kinase_rcpt_3_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF52">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25305">
        <property type="entry name" value="Ig_PDGFR_d4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500950">
        <property type="entry name" value="Alpha-PDGF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01832">
        <property type="entry name" value="VEGFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00240">
        <property type="entry name" value="RECEPTOR_TYR_KIN_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 27 32" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="18 31" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="21 33" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="28" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 35" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 27 32" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19 33" id="KW-0675">Receptor</keyword>
    <keyword evidence="40" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="36" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 33 35" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 35" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="36">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025395241" description="Platelet-derived growth factor receptor alpha" evidence="36">
        <location>
            <begin position="23"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="905"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="38">
        <location>
            <begin position="204"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="38">
        <location>
            <begin position="608"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="38">
        <location>
            <begin position="698"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="38">
        <location>
            <begin position="793"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="37">
        <location>
            <begin position="973"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="1391"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="34">
        <location>
            <begin position="1417"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="26">
        <location>
            <position position="1196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="952"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <begin position="979"/>
            <end position="987"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="980"/>
            <end position="987"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27 32">
        <location>
            <position position="1007"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="1055"/>
            <end position="1061"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="1200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="1201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="1214"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for interaction with phosphotyrosine-binding proteins" evidence="29">
        <location>
            <position position="1340"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="31">
        <location>
            <begin position="436"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="31">
        <location>
            <begin position="529"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="31">
        <location>
            <begin position="615"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="31">
        <location>
            <begin position="814"/>
            <end position="881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00029782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00064866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR500950-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PIRSR" id="PIRSR500950-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="RuleBase" id="RU000311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1465" mass="163321" checksum="C7515719C40C1400" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AFV8</accession>
    <name>A0A673AFV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylcholine transfer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">START domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">StAR-related lipid transfer protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pctp</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with ACOT13/THEM2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029998069.1">
        <property type="nucleotide sequence ID" value="XM_030142209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AFV8"/>
    <dbReference type="Ensembl" id="ENSSORT00005028407.1">
        <property type="protein sequence ID" value="ENSSORP00005027613.1"/>
        <property type="gene ID" value="ENSSORG00005013191.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424781"/>
    <dbReference type="CTD" id="58488"/>
    <dbReference type="InParanoid" id="A0A673AFV8"/>
    <dbReference type="OrthoDB" id="1295045at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008525">
        <property type="term" value="F:phosphatidylcholine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000017">
        <property type="entry name" value="Phosphatidylcholine transfer protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF39">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="START" evidence="12">
        <location>
            <begin position="23"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="213" mass="24925" checksum="564E8FE589F7391A" modified="2020-06-17" version="1">MSLRFSDADFQDAWMELDTPRLEGGWELFTETMGVQIHRLYDQETGLYEYKVFGVLSTCTPELCADVYMDLEYRKKWDGYVKELYEKDFDGQMAIYWEVKYPFPLSNRDYVYIRERRDLDVDGRKIWVILARSSPETPCPERSNVLRVKDYKQSVAMETDGGSGTKVFMNYFDNPGGNIPTWLVNWAAKSGVPGFLKDMQNACSKYKTYCDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGH6</accession>
    <name>A0A673AGH6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">slc44a5b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Choline transporter.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Choline/H+ antiporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>choline(out) + n H(+)(in) = choline(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75463"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AGH6"/>
    <dbReference type="FunCoup" id="A0A673AGH6">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028276.1">
        <property type="protein sequence ID" value="ENSSORP00005027487.1"/>
        <property type="gene ID" value="ENSSORG00005013133.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGH6"/>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027487.1"/>
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        </source>
    </evidence>
    <sequence length="668" mass="75316" checksum="58BD484D51D3247C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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    </dbReference>
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        <property type="entry name" value="CTTNBP2 N-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FILAMIN/GPBP-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09727">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cortactin-binding protein-2 N-terminal" evidence="9">
        <location>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF09727"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030704.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="583" mass="64115" checksum="90D17092CABA7D6B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVV2</accession>
    <name>A0A673AVV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nsun6</name>
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        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979008.1">
        <property type="nucleotide sequence ID" value="XM_030123148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AVV2"/>
    <dbReference type="SMR" id="A0A673AVV2"/>
    <dbReference type="FunCoup" id="A0A673AVV2">
        <property type="interactions" value="1096"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034362.1">
        <property type="protein sequence ID" value="ENSSORP00005033454.1"/>
        <property type="gene ID" value="ENSSORG00005015839.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411170"/>
    <dbReference type="CTD" id="221078"/>
    <dbReference type="InParanoid" id="A0A673AVV2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21150">
        <property type="entry name" value="PUA_NSun6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF34">
        <property type="entry name" value="TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="148"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="249"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="464" mass="50764" checksum="EBD6CDBB988DA817" modified="2020-06-17" version="1">MPIFPRISLKPEVCDHLKSIFLNKELLAAVGHQEAESRFQRLLMCLSHPPSYTCVRASTHLAPLEDIRQKLGEELKRQQQQMCSSSPEDVYAQILPHPHIPDVLLLPVDGPRPVQQLSSEVVVGAQCGNAVLRGAHVFAPGIIASPKYMKAGDLVSVFSDTEGKCTRGATSFQGLKVFVGNGVAEMDRSSIFCTDEPKKGIGVRMTEPLYQSPSFDGVLPSLAFLQNLPSVVVGHVLGPRPGERVLDMCAAPGGKTCHIAALMGDQGEVVALDRIRNKIDRIGQNAQMLHLHSIKVYCFNSTQAVSSDPSQDSEGPPFPPESFDRVLLDAPCSGLGQRPNMASTWSLKEICSYQPLQRKLIQAAVRLLRKGGVLVYSTCTVTLAENEEQVAWALRTFPCLTLQPQEPHIGAEGMLGAGLSPEQLRLLQRFSPELNWDQTESAVPLPHRANTDTIGFFIAKFLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AXA2</accession>
    <name>A0A673AXA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucosamine-6-phosphate deaminase</fullName>
            <ecNumber evidence="4">3.5.99.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673AXA2"/>
    <dbReference type="Ensembl" id="ENSSORT00005034787.1">
        <property type="protein sequence ID" value="ENSSORP00005033874.1"/>
        <property type="gene ID" value="ENSSORG00005016031.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AXA2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF5">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="12"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="197" mass="22405" checksum="12A3B2D7A4505A34" modified="2020-06-17" version="1">MRLVILDDYAQSSEWAAKYICNRIIQFNPSEDRYFTLGLPTGSTPYGCYQKLIQYHRSGDLSFKYVKTFNMDEYVGLPRSHPQSYHSYMWNNFFKHIDINPANAHILDGNADDLEEECQKYEEKIQEAGGIQLFVGGKNTWFQSVSRTRVKTLAKDTIMANARLFDNDLSKVPTMALTVGVGTVMDAKGGTIHPLCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B051</accession>
    <name>A0A673B051_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Golgi pH regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">si:ch73-390b10.2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>bromide(in) = bromide(out)</text>
            <dbReference type="Rhea" id="RHEA:75383"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>iodide(out) = iodide(in)</text>
            <dbReference type="Rhea" id="RHEA:66324"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Golgi pH regulator (TC 1.A.38) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B051"/>
    <dbReference type="FunCoup" id="A0A673B051">
        <property type="interactions" value="834"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036461.1">
        <property type="protein sequence ID" value="ENSSORP00005035511.1"/>
        <property type="gene ID" value="ENSSORG00005016761.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B051"/>
    <dbReference type="OrthoDB" id="264392at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ABA_GPCR_dom"/>
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    </dbReference>
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        <property type="entry name" value="GPHR/GTG"/>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 89-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GOLGI PH REGULATOR A-RELATED"/>
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        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
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        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
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        <location>
            <begin position="343"/>
            <end position="368"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Golgi pH regulator conserved" evidence="11">
        <location>
            <begin position="142"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Abscisic acid G-protein coupled receptor-like" evidence="10">
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            <begin position="277"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004141"/>
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            <dbReference type="ARBA" id="ARBA00009478"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="Pfam" id="PF12430"/>
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            <dbReference type="Pfam" id="PF12537"/>
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            <dbReference type="Ensembl" id="ENSSORP00005035511.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="455" mass="53086" checksum="97F9FA4E4721D407" modified="2020-06-17" version="1">MSFLVDSVIMFTSQVLFFGFGWLFFMRQLFKDYEVRQYVVQVVFSVTFAFSCTMFELIIFEILGALSSSSRYFHWKLNLYVILLVLIFVVPFYIGYFVVSNIRLLHRQRLLFACMVWFTFMYFFWKLGDPFPILSPKHGILSIEQLISRVGVIGVTLMALLSGFGAVNCPYTYMSYFLRNVTDSDILALERRLLQTMDMIVSKKKRIAMTRRQMYQRGEDQNKQTGFWGMIKSVTSTQTGSENLSLIQQEVDALEELSRQLFLETVDLQATKERIEYSKTFQGKYFNFLGYFFSIYCVWKIFMATINIVFDRVGKTDPVTRGIEITVNYLGIQFDVKFWSQHISFILVGIIIVTSIRGLLITLTKFFYAISSSKSSNVIVLVLAQIMGMYFVSSVLLMRMSMPLEYRSIVTEVLGELQFNFYHRWFDVIFLVSALSSILFLYLAHKQAPEKHMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0D0</accession>
    <name>A0A673B0D0_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">Zinc finger piccolo-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="F:structural constituent of presynaptic active zone"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="PICCOLO/BASSOON"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN PICCOLO"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
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            <end position="277"/>
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    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF05715"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="531" mass="56183" checksum="EAC8F66D606CDCAF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1B7</accession>
    <name>A0A673B1B7_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        </lineage>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="17"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="68" mass="7239" checksum="389CE063111A17FF" modified="2020-06-17" version="1">GRPITEGLVVRIPFLSLHSLNIKVEPGSIVAVVGHVGSGKSSLLSAMLGEMERRSGSACIKVREELIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2G1</accession>
    <name>A0A673B2G1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B2G1"/>
    <dbReference type="Ensembl" id="ENSSORT00005036489.1">
        <property type="protein sequence ID" value="ENSSORP00005035539.1"/>
        <property type="gene ID" value="ENSSORG00005016782.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2G1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="246" mass="27530" checksum="6656F7362091EC62" modified="2020-06-17" version="1">MPAEILLKMTCWFLGDSMCSLYVFLGWLSVSASVGDMVLISVDRYVAICDPLHYTHKVTVKRVKVCICLCWFCSGCFCFGILNDNLTHPGKYICQGECVISISHVAEVVDLVSTFILPISAIIALYMSVFVVAVSQARAMRSHITSITAQRSGKAVAKKSELKAARSLGVLVVVFLICFCPYYCVYLAADTSVTVTVYFYASFLMYINSCLNPLIYSLFYPWFRKSVKQIFTMQILQPDSQNINIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3M7</accession>
    <name>A0A673B3M7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GRAM domain containing 1Ba</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115432953</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B3M7"/>
    <dbReference type="Ensembl" id="ENSSORT00005037793.1">
        <property type="protein sequence ID" value="ENSSORP00005036826.1"/>
        <property type="gene ID" value="ENSSORG00005017094.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3M7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000008">
        <property type="entry name" value="GRAM domain containing 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF3">
        <property type="entry name" value="PROTEIN ASTER-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="651"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="402"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="285"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="356"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="771" mass="89084" checksum="1769356ACBA7D12B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B434</accession>
    <name>A0A673B434_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Fucosyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115431962</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes alpha(1-&gt;3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1-&gt;4)GlcNAc) glycan attached to N- or O-linked glycoproteins. Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins. Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis. In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = a beta-D-galactosyl-(1-&gt;4)-[alpha-L-fucosyl-(1-&gt;3)]-N-acetyl-beta-D-glucosaminyl derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14257"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:137941"/>
            <dbReference type="EC" id="2.4.1.152"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:14258"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56076"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:136545"/>
            <dbReference type="ChEBI" id="CHEBI:139509"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:56077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68044"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:145343"/>
            <dbReference type="ChEBI" id="CHEBI:176900"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:68045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc6S derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc6S derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62004"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:145344"/>
            <dbReference type="ChEBI" id="CHEBI:145345"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:62005"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Golgi apparatus</location>
            <location evidence="18">Golgi stack membrane</location>
            <topology evidence="18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A673B434"/>
    <dbReference type="Ensembl" id="ENSSORT00005037949.1">
        <property type="protein sequence ID" value="ENSSORP00005036981.1"/>
        <property type="gene ID" value="ENSSORG00005017370.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B434"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017083">
        <property type="term" value="F:4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF132">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="84"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="161"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="326" mass="37578" checksum="64C27CC4259EDBDE" modified="2020-06-17" version="1">MRRAASRLVSRTCSSVCVATVGFVLLLGVCLLYLPDPFSLEPLVSVSDDNSEVTLLIWMHPFGQYRKLPHCGGLYGIEGCTLTDKPRPRAQKWVWMNYESPAHTPGLWRFEGVFNLTMTYRTDSDIFLPYGYLIPRGRRAKGLQNRYARPLHVLSRSLLRPRLVAWVISNWSESQARVAFYYQLRRYLQVDVFGRAGRPLPAGSGNVVRLVSRYQFYLALENSPHTDYITEKLWNAVLAGAVPVVLGPSRQNYERFLPPPGLYPRRRFSNCSSFGRYLLMLKRNPIQLKRHLDWRGSYSVHQPSFWAEHYCTVCRAVKKTRGKATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4M8</accession>
    <name>A0A673B4M8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dual specificity phosphatase 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dusp16</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030005809.1">
        <property type="nucleotide sequence ID" value="XM_030149949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B4M8"/>
    <dbReference type="FunCoup" id="A0A673B4M8">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036403.1">
        <property type="protein sequence ID" value="ENSSORP00005035453.1"/>
        <property type="gene ID" value="ENSSORG00005016737.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430062"/>
    <dbReference type="CTD" id="80824"/>
    <dbReference type="InParanoid" id="A0A673B4M8"/>
    <dbReference type="OrthoDB" id="165342at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000016">
        <property type="entry name" value="Dual specificity phosphatase 16 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000050">
        <property type="entry name" value="Dual specificity phosphatase 16 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF343">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="7">
        <location>
            <begin position="41"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="177"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="243"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="405"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="443"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="593"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="655" mass="71006" checksum="00256FF10313E2DD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B560</accession>
    <name>A0A673B560_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HOOK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ccdc88ab</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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</entry>
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</entry>
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    <accession>A0A673B8P3</accession>
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        <submittedName>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="DAPIN"/>
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        <property type="entry name" value="NACHT_NTPase"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50824"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038220.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="797" mass="92075" checksum="2B485FEFF61F6B05" modified="2020-06-17" version="1"> </sequence>
</entry>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1081" mass="122403" checksum="E3056AE947469D2C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BD39</accession>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="126"/>
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    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="61"/>
            <end position="123"/>
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        <source>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="126" mass="14249" checksum="C0526DF7CF8F69ED" modified="2020-06-17" version="1">SQRVENGDMNWIIPGKILAFSSPHPLRLNRKLYEGRRFEDAGFEHHDLFLDGTTPSDLIIRRFLHICESTKGAVAVHCKAGLGRTGTLIGCYLMKHFRFTAAEAIAWIRICRPGSIIGPQQNFLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEG8</accession>
    <name>A0A673BEG8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytokine like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cytl1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029989500.1">
        <property type="nucleotide sequence ID" value="XM_030133640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BEG8"/>
    <dbReference type="Ensembl" id="ENSSORT00005040004.1">
        <property type="protein sequence ID" value="ENSSORP00005039002.1"/>
        <property type="gene ID" value="ENSSORG00005018234.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419032"/>
    <dbReference type="InParanoid" id="A0A673BEG8"/>
    <dbReference type="OrthoDB" id="9899179at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029253">
        <property type="entry name" value="CYTL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15974">
        <property type="entry name" value="CYTOKINE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15974:SF0">
        <property type="entry name" value="CYTOKINE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15153">
        <property type="entry name" value="CYTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025480537" description="Cytokine like 1" evidence="1">
        <location>
            <begin position="21"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16979" checksum="C30791C17738D06E" modified="2020-06-17" version="1">MRLGLAALLCFFSLSWPSECAPPTCYSRALGLSKEIMTLLDKIHTYHRTKPCAEILPTIFLDVHNSCVTAKLRDFLYVVLNHPNQYCKDRPRMVLLKRKIQNLYTIITRICYRDLVFFTDDCVAIDTGHSSPHYAEDRLQLLQEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BHQ0</accession>
    <name>A0A673BHQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Growth factor receptor-bound protein 2b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">grb2b</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BHQ0"/>
    <dbReference type="FunCoup" id="A0A673BHQ0">
        <property type="interactions" value="2345"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043164.1">
        <property type="protein sequence ID" value="ENSSORP00005042091.1"/>
        <property type="gene ID" value="ENSSORG00005019519.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BHQ0"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09941">
        <property type="entry name" value="SH2_Grb2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11949">
        <property type="entry name" value="SH3_GRB2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11946">
        <property type="entry name" value="SH3_GRB2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000067">
        <property type="entry name" value="Growth factor receptor-bound protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000076">
        <property type="entry name" value="Growth factor receptor-bound protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000022">
        <property type="entry name" value="Growth factor receptor-bound protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035643">
        <property type="entry name" value="GRB2_C_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035641">
        <property type="entry name" value="GRB2_N_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="60"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="25235" checksum="B17989C522799D84" modified="2020-06-17" version="1">MEAIAKYDFKATADDELSFKRGEILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKAHPWFFGKIPRAKAEEMLNKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQHPTYVQALFDFDPQEEGELGFRRGDFIQVLDNSDPNWWKGGCHGQTGMFPRNYVTPVNRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BHV9</accession>
    <name>A0A673BHV9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Muscle segment homeobox 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115426667</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Msh homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BHV9"/>
    <dbReference type="Ensembl" id="ENSSORT00005042387.1">
        <property type="protein sequence ID" value="ENSSORP00005041325.1"/>
        <property type="gene ID" value="ENSSORG00005019233.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BHV9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043049">
        <property type="term" value="P:otic placode formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000134">
        <property type="entry name" value="Homeobox protein MSX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050674">
        <property type="entry name" value="Msh_Homeobox_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24338">
        <property type="entry name" value="HOMEOBOX PROTEIN MSX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24338:SF0">
        <property type="entry name" value="MUSCLE SEGMENTATION HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="116"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="118"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="251" mass="27761" checksum="ABA2F1025AABFE85" modified="2020-06-17" version="1">KKKRKKSKNKSMSSFGERRDGVSADEDAQPPQPKTRTAQVFPLPFSVEALMSDTGRPLAATDTDTGSSDSLSDDQEPICIKSEPTERDVAPWISSPTMTTSPRQLSPNICSLRKHKTNRKPRTPFTTTQLLALERKFHQKQYLSIAERAEFSSSLSLSETQVKIWFQNRRAKAKRLQEAEVEKIKLAAGVGPKAMFYSGFSSLGLPVSVNLQTGPASLYGLGCSYSRSTLLPAAHLAMYASPVSRRLFNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJ95</accession>
    <name>A0A673BJ95_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BJ95"/>
    <dbReference type="Ensembl" id="ENSSORT00005042897.1">
        <property type="protein sequence ID" value="ENSSORP00005041825.1"/>
        <property type="gene ID" value="ENSSORG00005019422.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJ95"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="122" mass="14014" checksum="A53B06D02B93CCE6" modified="2020-06-17" version="1">MVTISDVHGDQESWCGDEDELKTPETDVRDWKELIVTDVFTARLKKKKKEDRNRVRLFVTPDALSSHNQHHNPENKQHRQPYFAQAGGVTVRHPVKHTTKSGDKTRILIRLLADKYSALCPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKN6</accession>
    <name>A0A673BKN6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="26">Low density lipoprotein receptor a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ldlra</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Endomembrane system</location>
            <topology evidence="20">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BKN6"/>
    <dbReference type="FunCoup" id="A0A673BKN6">
        <property type="interactions" value="235"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043413.1">
        <property type="protein sequence ID" value="ENSSORP00005042335.1"/>
        <property type="gene ID" value="ENSSORG00005019623.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BKN6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000162">
        <property type="entry name" value="LDL receptor related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000124">
        <property type="entry name" value="Low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000116">
        <property type="entry name" value="Low-density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000002">
        <property type="entry name" value="low-density lipoprotein receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000113">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000025">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1220.10">
        <property type="entry name" value="EGF-type module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF15">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="17" id="KW-0168">Coated pit</keyword>
    <keyword evidence="14 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 21" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="15" id="KW-1207">Sterol metabolism</keyword>
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    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="350"/>
        </location>
    </feature>
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        <location>
            <begin position="400"/>
            <end position="446"/>
        </location>
    </feature>
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            <end position="489"/>
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    <feature type="repeat" description="LDL-receptor class B" evidence="23">
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        <location>
            <begin position="534"/>
            <end position="578"/>
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        <location>
            <begin position="695"/>
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        </location>
    </feature>
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        <location>
            <begin position="724"/>
            <end position="733"/>
        </location>
    </feature>
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        <location>
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            <end position="753"/>
        </location>
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        <location>
            <begin position="770"/>
            <end position="782"/>
        </location>
    </feature>
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        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="67"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="86"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="106"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="177"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="197"/>
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    <feature type="disulfide bond" evidence="22">
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    <feature type="disulfide bond" evidence="22">
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    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
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        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="802" mass="88520" checksum="C88700A0B09821F8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMK4</accession>
    <name>A0A673BMK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115433933</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Structural component of lens fiber gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BMK4"/>
    <dbReference type="Ensembl" id="ENSSORT00005042904.1">
        <property type="protein sequence ID" value="ENSSORP00005041832.1"/>
        <property type="gene ID" value="ENSSORG00005019425.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BMK4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000002">
        <property type="entry name" value="Gap junction protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034634">
        <property type="entry name" value="Connexin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF12">
        <property type="entry name" value="GAP JUNCTION ALPHA-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
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        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118220">
        <property type="entry name" value="Connexin43"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
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            <end position="76"/>
        </location>
    </feature>
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            <end position="239"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
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            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045560"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="427" mass="47436" checksum="F2DA09A37895F86E" modified="2020-06-17" version="1">MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEEVWGDEQSDFTCNTQQPGCENVCYDEAFPISHIRFWVLQIIFVSTPTLIYLGHVLHIVRMEEKRREREEELRKAGRHQEDHDPLYHNGVGDGGGGGGKKEKPPIRDEHGKIRIRGALLRTYIFNIIFKTLFEVGFILGQYFLYGFHLRPLYKCGRWPCPNTVDCFISRPTEKTIFIIFMLVVACVSLLLNLLEIYHLGWKKVKQGVTNEFAPDAESLLLSADERGDAETIPEQTSPSVLDCLPVYTSVSVVAGGAAFHPDDFLLEAIPASFYENGEKVGNNGQVTAMEQNWSNMELELQALDGKNSSSSCPPLPSSPTSISSSPQEEMTPPPSTSAPSAPPYDDFTVVTKAEMHQPPAAVATDIRKPSRANKSSGVRARPDDLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNI4</accession>
    <name>A0A673BNI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">mtmr4</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.95" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673BNI4"/>
    <dbReference type="Ensembl" id="ENSSORT00005043241.1">
        <property type="protein sequence ID" value="ENSSORP00005042167.1"/>
        <property type="gene ID" value="ENSSORG00005015868.1"/>
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    <dbReference type="InParanoid" id="A0A673BNI4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061952">
        <property type="term" value="P:midbody abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15733">
        <property type="entry name" value="FYVE_MTMR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000073">
        <property type="entry name" value="myotubularin-related protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046978">
        <property type="entry name" value="MTMR4_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
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        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 4"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="155"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="379"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="16">
        <location>
            <begin position="1110"/>
            <end position="1170"/>
        </location>
    </feature>
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    <feature type="region of interest" description="Disordered" evidence="14">
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    </feature>
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        <location>
            <begin position="1072"/>
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        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="697"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="747"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
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            <end position="863"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="864"/>
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        <location>
            <begin position="1084"/>
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    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
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        </location>
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        <location>
            <begin position="323"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>substrate</name>
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        <location>
            <begin position="348"/>
            <end position="349"/>
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        <ligand>
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            <end position="416"/>
        </location>
        <ligand>
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    <sequence length="1191" mass="132169" checksum="EF750FF70FA11682" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BV50</accession>
    <name>A0A673BV50_9TELE</name>
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        <recommendedName>
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        </lineage>
    </organism>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_030008836.1">
        <property type="nucleotide sequence ID" value="XM_030152976.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030008837.1">
        <property type="nucleotide sequence ID" value="XM_030152977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BV50"/>
    <dbReference type="FunCoup" id="A0A673BV50">
        <property type="interactions" value="1119"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045172.1">
        <property type="protein sequence ID" value="ENSSORP00005044053.1"/>
        <property type="gene ID" value="ENSSORG00005020308.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432140"/>
    <dbReference type="CTD" id="10298"/>
    <dbReference type="InParanoid" id="A0A673BV50"/>
    <dbReference type="OrthoDB" id="1022360at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06657">
        <property type="entry name" value="STKc_PAK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000073">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF9">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="14">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="390"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="92"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="660" mass="73964" checksum="844A88B904B1D2D2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0D2</accession>
    <name>A0A673C0D2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115433790</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C0D2"/>
    <dbReference type="Ensembl" id="ENSSORT00005047894.1">
        <property type="protein sequence ID" value="ENSSORP00005046729.1"/>
        <property type="gene ID" value="ENSSORG00005021379.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C0D2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903979">
        <property type="term" value="P:negative regulation of microglial cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031643">
        <property type="term" value="P:positive regulation of myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000004">
        <property type="entry name" value="Cystatin C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042886">
        <property type="entry name" value="Cystatin-F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47141">
        <property type="entry name" value="CYSTATIN-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47141:SF1">
        <property type="entry name" value="CYSTATIN-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025552194" description="Cystatin domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="4">
        <location>
            <begin position="34"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="156" mass="18024" checksum="470A14FE602BF8F6" modified="2020-06-17" version="1">MMAVMRRTLLAVLLGILEASSLAVQGHQGRHVHMIPGAPSNVSLNDRRLHQVVLTTAYTYNNQSNDAFLFRPSSVHRAQRQIVKGLRYIIDLDVSRTVCRKRVPIPDLSQCEFQPEGRLHQTFRCHTEVWFIPWTNQTTMQVFYCKDTESDVYSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1K1</accession>
    <name>A0A673C1K1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Si:dkey-33c12.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C1K1"/>
    <dbReference type="FunCoup" id="A0A673C1K1">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050303.1">
        <property type="protein sequence ID" value="ENSSORP00005049106.1"/>
        <property type="gene ID" value="ENSSORG00005022317.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1K1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099160">
        <property type="term" value="C:postsynaptic intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099184">
        <property type="term" value="F:structural constituent of postsynaptic intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033693">
        <property type="term" value="P:neurofilament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF4">
        <property type="entry name" value="INTERMEDIATE FILAMENT PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="77"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="95"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="299"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="390" mass="45025" checksum="9EFD67C43CA6D926" modified="2020-06-17" version="1">MSYDSYFSYRRPWDSYRGSRSTKSSVTSSLYSAPRAAPSGKRILRLASSSLPDGSERLDLAQASSLNTELLGLRSQEREQLVDLNDRFATYIEKVRHLELQNRALLSELEALRRRQNHPSRLQAVYEGEARSLRAMIDSENGEKMRMEAERDYLRDVYEQMKERCEEEARRRMDAEDALQRAKEEAGRALLSNCDAEATVVSLCDEMAFLKKVFAEEQAELQAQLQVANISVDVEVSRPDLSTALRDIRAQYERLANKNMQAAEDWYKSKFASVAEMASKNNEAVHAIREETMEYRRLLQSRSSEIEALRNVIDSLNKQLEELEDTQAKEVSKYQVRICELERDITEAKQEMARYLKEYQDLLNVKMALDIEIAYMITACSGVRLTLSFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1Q6</accession>
    <name>A0A673C1Q6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fatty acid synthase</fullName>
            <ecNumber evidence="5">1.1.1.100</ecNumber>
            <ecNumber evidence="2">1.3.1.39</ecNumber>
            <ecNumber evidence="8">2.3.1.38</ecNumber>
            <ecNumber evidence="9">2.3.1.39</ecNumber>
            <ecNumber evidence="7">2.3.1.41</ecNumber>
            <ecNumber evidence="4">2.3.1.85</ecNumber>
            <ecNumber evidence="3">3.1.2.14</ecNumber>
            <ecNumber evidence="6">4.2.1.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="73" type="primary">fasn</name>
    </gene>
    <organism evidence="73 74">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="73" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="73" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="73" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="36">Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>(2E)-butenoyl-[ACP] + NADPH + H(+) = butanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41812"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:41813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="67">
            <text>(2E)-decenoyl-[ACP] + NADPH + H(+) = decanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41864"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="67">
            <dbReference type="Rhea" id="RHEA:41865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="50">
            <text>(2E)-dodecenoyl-[ACP] + NADPH + H(+) = dodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="50">
            <dbReference type="Rhea" id="RHEA:41881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>(2E)-hexadecenoyl-[ACP] + NADPH + H(+) = hexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41912"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:41913"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>(2E)-hexenoyl-[ACP] + NADPH + H(+) = hexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41832"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="46">
            <dbReference type="Rhea" id="RHEA:41833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="60">
            <text>(2E)-octadecenoyl-[ACP] + NADPH + H(+) = octadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41928"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="Rhea" id="RHEA-COMP:9656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
            <dbReference type="ChEBI" id="CHEBI:78495"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="60">
            <dbReference type="Rhea" id="RHEA:41929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="53">
            <text>(2E)-octenoyl-[ACP] + NADPH + H(+) = octanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41848"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="53">
            <dbReference type="Rhea" id="RHEA:41849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="62">
            <text>(2E)-tetradecenoyl-[ACP] + NADPH + H(+) = tetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41896"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="62">
            <dbReference type="Rhea" id="RHEA:41897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41808"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:41809"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41860"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:41861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41876"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:41877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:41909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41828"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:41829"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41924"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:41925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41844"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:41845"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41892"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:41893"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="47">
            <text>3-oxobutanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxybutanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41804"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="47">
            <dbReference type="Rhea" id="RHEA:41805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>3-oxodecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41856"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:41857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="63">
            <text>3-oxododecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41872"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="63">
            <dbReference type="Rhea" id="RHEA:41873"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="64">
            <text>3-oxohexadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="64">
            <dbReference type="Rhea" id="RHEA:41905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="55">
            <text>3-oxohexanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41824"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="55">
            <dbReference type="Rhea" id="RHEA:41825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>3-oxooctadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41920"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:41921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="65">
            <text>3-oxooctanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41840"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="65">
            <dbReference type="Rhea" id="RHEA:41841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="59">
            <text>3-oxotetradecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxytetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41888"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="59">
            <dbReference type="Rhea" id="RHEA:41889"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="40">
            <dbReference type="Rhea" id="RHEA:17399"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:13098"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="56">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="56">
            <dbReference type="Rhea" id="RHEA:22566"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="54">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="54">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H(+) = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.85"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="48">
            <text>acetyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41800"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="48">
            <dbReference type="Rhea" id="RHEA:41801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="66">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="66">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="61">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="61">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="58">
            <text>hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41932"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="58">
            <dbReference type="Rhea" id="RHEA:41933"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="49">
            <text>hexadecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="49">
            <dbReference type="Rhea" id="RHEA:41917"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="57">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="57">
            <dbReference type="Rhea" id="RHEA:41789"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="52">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="52">
            <dbReference type="Rhea" id="RHEA:41793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="68">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="68">
            <dbReference type="Rhea" id="RHEA:41853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="51">
            <text>tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30123"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="51">
            <dbReference type="Rhea" id="RHEA:30124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41900"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:41901"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="5"/>
    <dbReference type="EC" id="1.3.1.39" evidence="2"/>
    <dbReference type="EC" id="2.3.1.38" evidence="8"/>
    <dbReference type="EC" id="2.3.1.39" evidence="9"/>
    <dbReference type="EC" id="2.3.1.41" evidence="7"/>
    <dbReference type="EC" id="2.3.1.85" evidence="4"/>
    <dbReference type="EC" id="3.1.2.14" evidence="3"/>
    <dbReference type="EC" id="4.2.1.59" evidence="6"/>
    <dbReference type="FunCoup" id="A0A673C1Q6">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049121.1">
        <property type="protein sequence ID" value="ENSSORP00005047935.1"/>
        <property type="gene ID" value="ENSSORG00005021885.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1Q6"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004313">
        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="Ketoacyl_synth_AS"/>
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        <property type="entry name" value="PKS_PP-bd"/>
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        <property type="entry name" value="PP-bd_ACP"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="entry name" value="ketoacyl-synt"/>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    <dbReference type="PROSITE" id="PS00606">
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    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
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        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
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        <property type="entry name" value="PKS_MFAS_DH"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="23" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="25" id="KW-0456">Lyase</keyword>
    <keyword evidence="26" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-0521">NADP</keyword>
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    <keyword evidence="11" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="74" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
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        <location>
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    </feature>
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        <location>
            <begin position="2112"/>
            <end position="2192"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="69">
        <location>
            <begin position="847"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="69">
        <location>
            <begin position="986"/>
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        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="69">
        <location>
            <position position="881"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="69">
        <location>
            <position position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012480"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012873"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013167"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
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    </evidence>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="18">
        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    <evidence type="ECO:0000256" key="28">
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
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    <evidence type="ECO:0000256" key="31">
        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00023401"/>
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    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00023402"/>
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    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00044883"/>
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    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00047300"/>
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    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00047400"/>
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    <evidence type="ECO:0000256" key="41">
        <source>
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    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00047451"/>
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    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00047500"/>
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    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00047578"/>
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    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00047810"/>
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    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00047897"/>
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    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
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    <evidence type="ECO:0000256" key="48">
        <source>
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    <evidence type="ECO:0000256" key="49">
        <source>
            <dbReference type="ARBA" id="ARBA00048051"/>
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    <evidence type="ECO:0000256" key="50">
        <source>
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    <evidence type="ECO:0000256" key="51">
        <source>
            <dbReference type="ARBA" id="ARBA00048289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="52">
        <source>
            <dbReference type="ARBA" id="ARBA00048404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="53">
        <source>
            <dbReference type="ARBA" id="ARBA00048420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="54">
        <source>
            <dbReference type="ARBA" id="ARBA00048506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="55">
        <source>
            <dbReference type="ARBA" id="ARBA00048571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="56">
        <source>
            <dbReference type="ARBA" id="ARBA00048650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="57">
        <source>
            <dbReference type="ARBA" id="ARBA00048691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="58">
        <source>
            <dbReference type="ARBA" id="ARBA00048704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="59">
        <source>
            <dbReference type="ARBA" id="ARBA00048935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="60">
        <source>
            <dbReference type="ARBA" id="ARBA00049019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="61">
        <source>
            <dbReference type="ARBA" id="ARBA00049109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="62">
        <source>
            <dbReference type="ARBA" id="ARBA00049171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="63">
        <source>
            <dbReference type="ARBA" id="ARBA00049263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="64">
        <source>
            <dbReference type="ARBA" id="ARBA00049414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="65">
        <source>
            <dbReference type="ARBA" id="ARBA00049422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="66">
        <source>
            <dbReference type="ARBA" id="ARBA00049449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="67">
        <source>
            <dbReference type="ARBA" id="ARBA00049521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="68">
        <source>
            <dbReference type="ARBA" id="ARBA00049533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="69">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="70">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="71">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="72">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="73">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="74">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2475" mass="269770" checksum="E22D52A085CD2E29" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C214</accession>
    <name>A0A673C214_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Plakophilin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pkp2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C214"/>
    <dbReference type="FunCoup" id="A0A673C214">
        <property type="interactions" value="900"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050501.1">
        <property type="protein sequence ID" value="ENSSORP00005049301.1"/>
        <property type="gene ID" value="ENSSORG00005022398.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C214"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002934">
        <property type="term" value="P:desmosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045110">
        <property type="term" value="P:intermediate filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF25">
        <property type="entry name" value="PLAKOPHILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="240"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="677" mass="75807" checksum="A0CD817ED75576C8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4B3</accession>
    <name>A0A673C4B3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Development and differentiation-enhancing factor-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">asap3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Promotes cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C4B3"/>
    <dbReference type="Ensembl" id="ENSSORT00005049277.1">
        <property type="protein sequence ID" value="ENSSORP00005048089.1"/>
        <property type="gene ID" value="ENSSORG00005021845.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C4B3"/>
    <dbReference type="OrthoDB" id="435430at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13251">
        <property type="entry name" value="PH_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000002">
        <property type="entry name" value="arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000001">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000006">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000036">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF1">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="18">
        <location>
            <begin position="305"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="19">
        <location>
            <begin position="421"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="616"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="948"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="696"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="753"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="917"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="782"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="800"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="844"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="873"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="923"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1010" mass="112377" checksum="57B8915EAEE8B232" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C526</accession>
    <name>A0A673C526_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">klhl40b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">A band</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
            <location evidence="2">I band</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KLHL40 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C526"/>
    <dbReference type="FunCoup" id="A0A673C526">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051708.1">
        <property type="protein sequence ID" value="ENSSORP00005050496.1"/>
        <property type="gene ID" value="ENSSORG00005022880.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C526"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031674">
        <property type="term" value="C:I band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18340">
        <property type="entry name" value="BTB_POZ_KLHL40_KBTBD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000006">
        <property type="entry name" value="Kelch repeat and BTB domain-containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000037">
        <property type="entry name" value="Kelch-like family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030607">
        <property type="entry name" value="KLHL40_BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF22">
        <property type="entry name" value="KELCH-LIKE PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="263"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="614" mass="69234" checksum="9EF8600F7A7DD320" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5R5</accession>
    <name>A0A673C5R5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Transmembrane emp24 domain-containing protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">p24 family protein gamma-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">p27</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">tmed7</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Appears to play a role in the biosynthesis of secreted cargo including processing and post-translational modifications.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasmic vesicle</location>
            <location evidence="16">COPI-coated vesicle membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Cytoplasmic vesicle</location>
            <location evidence="17">COPII-coated vesicle membrane</location>
            <topology evidence="17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Membrane</location>
            <topology evidence="21">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C5R5"/>
    <dbReference type="FunCoup" id="A0A673C5R5">
        <property type="interactions" value="1575"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049407.1">
        <property type="protein sequence ID" value="ENSSORP00005048217.1"/>
        <property type="gene ID" value="ENSSORG00005022018.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C5R5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    <sequence length="217" mass="24675" checksum="8C96C448FD631F92" modified="2020-06-17" version="1">MCSVMYGSPRVLLQVLWAQLLCGWVLGSELTFELPDNAKQCFYEDIIIGTKCTLEFQVVTGGHYDVDCRLEDPDGTTLYKEMKKQYDSFTFTAAKNGTYKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNTRVAFWSIGEAIILLVVSISQVVLLRSFFSDKKTTTTRVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6J7</accession>
    <name>A0A673C6J7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">1-acyl-sn-glycerol-3-phosphate acyltransferase delta</fullName>
            <ecNumber evidence="6">2.3.1.51</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">1-acylglycerol-3-phosphate O-acyltransferase 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Lysophosphatidic acid acyltransferase delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">agpat4</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Exhibits high acyl-CoA specificity for polyunsaturated fatty acyl-CoA, especially docosahexaenoyl-CoA (22:6-CoA, DHA-CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z-octadecenoyl)-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:55312"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:74298"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:138723"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:55313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphate = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:55300"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:74298"/>
            <dbReference type="ChEBI" id="CHEBI:82928"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:55301"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-octadecanoyl-sn-glycero-3-phosphate + (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA = 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:55308"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:74298"/>
            <dbReference type="ChEBI" id="CHEBI:74565"/>
            <dbReference type="ChEBI" id="CHEBI:77130"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:55309"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-octadecanoyl-sn-glycero-3-phosphate + (9Z,12Z)-octadecadienoyl-CoA = 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:55304"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:74565"/>
            <dbReference type="ChEBI" id="CHEBI:77098"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:55305"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:19710"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673C6J7"/>
    <dbReference type="Ensembl" id="ENSSORT00005050076.1">
        <property type="protein sequence ID" value="ENSSORP00005048884.1"/>
        <property type="gene ID" value="ENSSORG00005022242.1"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF8">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="311"/>
            <end position="330"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="26">
        <location>
            <begin position="90"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000300"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036892"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040981"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041272"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042940"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="388" mass="45160" checksum="3A1047A9A48F09B8" modified="2020-06-17" version="1">MGLLQLLKSQVLCHLFICFVFLVSGIIVNMLQLCTLPLWLVNKQLFRRVNIRLAYCISSQMVAVLEWWSGTECTLYTDPKSYSHYGKENAIVVLNHNFEIDFMCGWTFCDRFGVLGSSKVLAKKELAYVPVIGWMWYFLEIVFCKRKWEEDRKTVAQSLQNLQDYPENFWFLLYCEGTRFTPKKHEISMQVAESKGLPKLKYHLLPRTKGFWVTVNNLRGKVAAVYDSTLNFRDNEAPTLHGVLNGKKYHADLYVRRIPLESIPEGEAECAAWLHKLYQEKDSFQEHYAQTGRFPGPITNPPRRPWALINWLFWCGLFFYHLVLLFVQLLSSGSMLTITASLAFCVAVSVGVRWMIGQTEIDRGSTYGNKDKTNKHWNTFYSDHRRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCH8</accession>
    <name>A0A673CCH8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Trans-2,3-enoyl-CoA reductase-like 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115411632</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCH8"/>
    <dbReference type="Ensembl" id="ENSSORT00005053018.1">
        <property type="protein sequence ID" value="ENSSORP00005051785.1"/>
        <property type="gene ID" value="ENSSORG00005023377.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCH8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049127">
        <property type="entry name" value="TECR-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF59">
        <property type="entry name" value="STEROID 5-ALPHA REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21696">
        <property type="entry name" value="TECR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="TECR-like N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="144"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="34618" checksum="388980F329F2384F" modified="2020-06-17" version="1">MCFPQVEVLDAKTREQLCFLDKVCYHRTDPHWYPARQALKLDPRGKSLRDDEILQNLPVGTTATMYFRDLGPQLGWTMVFLAEYIGPLLTYLLFYFRVPYIYSHRYAFTSSPHPVVTLACACHTSHYIKRLVETIFVHRFSHGTMPLRTIVRNCVYYWGFSAWLAYYINHPLYTPPSYGELQVNYALAIFVICELGNFSIHLTLNNLRGEGESRRFPVPTKNPFTWLFFFVSCPNYTYEVGAWVSFSIMTQCLPVAFYTLLGFIQMTIWAKGKHKAYNREFKDYPSLRMAIIPLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCM1</accession>
    <name>A0A673CCM1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sec22c</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in vesicle transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979184.1">
        <property type="nucleotide sequence ID" value="XM_030123324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CCM1"/>
    <dbReference type="FunCoup" id="A0A673CCM1">
        <property type="interactions" value="632"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051692.1">
        <property type="protein sequence ID" value="ENSSORP00005050482.1"/>
        <property type="gene ID" value="ENSSORG00005022871.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411261"/>
    <dbReference type="CTD" id="9117"/>
    <dbReference type="InParanoid" id="A0A673CCM1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059071">
        <property type="entry name" value="SEC22a-c_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043546">
        <property type="entry name" value="Sec22a/c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46258">
        <property type="entry name" value="LONGIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46258:SF2">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25970">
        <property type="entry name" value="SEC22a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025665561" description="Longin domain-containing protein" evidence="12">
        <location>
            <begin position="16"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="13">
        <location>
            <begin position="8"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="303" mass="34092" checksum="DFDEE4C154022345" modified="2020-06-17" version="1">MSLILFAFVVRVRDGLPLSASTDFEHNRELQERKQQLRTISKTLVRFPDRGTVKGQQLNIHFISSEGVSYMTVCHCSLPVAKAFCFLEDLRWEFTACFNSTVVTLAARPYPFLEFDSTIQKLKQQYNQNGGPALEVTLAEVQEDLKIHPPQVIPLEDIELSNGTANGHMEQGPGSGQNLRLEPVTAPGILSLVLNIMCASLNVIRGVHLIEYTFQDDYEGIWNVVAFLLAFVCCVSQCHLYLFHSSLKKFKSLTLLIVIVLCNFYLLGLRNMWQLIFHISVASLSTALILSRKLQDKPNDCGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CH61</accession>
    <name>A0A673CH61_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CH61"/>
    <dbReference type="Ensembl" id="ENSSORT00005054756.1">
        <property type="protein sequence ID" value="ENSSORP00005053500.1"/>
        <property type="gene ID" value="ENSSORG00005024059.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CH61"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="4">
        <location>
            <begin position="31"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="4">
        <location>
            <begin position="118"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="256" mass="29504" checksum="BA7DE81E2AA8CF0E" modified="2020-06-17" version="1">QIWMHLEVLHIQKVYSSFIKVTLLSPTNPTVSKVTVEKGGSISIPCFYESKYTDNEKYLCKGCKWKICSYEVKTNQYNSTKYLISDDQNQGVVTWTINDLTEEDTDCYWCFETSSVNTHEVTGFIGDQITIDCHYRGSKQIKWCRTGGEGPTSCIKDSGKKDDTKVTINRNTPGVITVTMSELRERSSGWYWCTDEDLQMPVHVTIKERPTTITYSTVTKTERNINILLFYTSAALLHYHLSLISDHVYYHNHFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CI98</accession>
    <name>A0A673CI98_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">G protein-coupled receptor 137Bb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gpr137bb</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CI98"/>
    <dbReference type="Ensembl" id="ENSSORT00005054288.1">
        <property type="protein sequence ID" value="ENSSORP00005053034.1"/>
        <property type="gene ID" value="ENSSORG00005023886.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CI98"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045779">
        <property type="term" value="P:negative regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21476">
        <property type="entry name" value="7tm_GPR137B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029723">
        <property type="entry name" value="GPR137"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146:SF6">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 137BB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="428" mass="48614" checksum="7ED1D50A78D71535" modified="2020-06-17" version="1">MEAPDGNPAQEQHHAGNGSHLPPPPTITPAIPPSVKLGLTVAYTVFYSLLFAFVYAQLWLVLRYRHKRFSYQTAFLFLCLIWAALRALLFSFYFRDCVTANALAPFAFWILYCFPVCLQFFTLSLMNLYCAQVYFKAKSKYSPSLLKYRLPLYLVFLGISLLFLVVNLVCALLVKMTASDVKTIVLVRVTINDSLFVLCAISLSVCLYKVAKMSLANIYLESKGTSACQVTLIGVTVVLLYASRACYNLVVLALTDIETINSFDYDWYNVSDQADLRSSLGDAGYIVFGVILFVWELLPTSLVVFFFRVRRPPQDRNTVGLPNHVLSSRGYFFDNPNRYNSDDDLAWSIPPQNASARYKHLQSYRMITSTTLQIVLSFYNEEKITKNEDLVPKRNATSVIWNYFSCKKDHTETCSVSTVPCNCCHNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJ80</accession>
    <name>A0A673CJ80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115422188</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Cell membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJ80"/>
    <dbReference type="Ensembl" id="ENSSORT00005055528.1">
        <property type="protein sequence ID" value="ENSSORP00005054255.1"/>
        <property type="gene ID" value="ENSSORG00005023938.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJ80"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007398">
        <property type="term" value="P:ectoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001841">
        <property type="term" value="P:neural tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000022">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000027">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000045">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="935" mass="103278" checksum="2A781273C271BCCE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJ97</accession>
    <name>A0A673CJ97_9TELE</name>
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        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="22" type="primary">mark3a</name>
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    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
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            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="MARK1-4_cat"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="UBA"/>
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        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
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    <dbReference type="PANTHER" id="PTHR24346:SF98">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
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    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="46"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="20">
        <location>
            <begin position="313"/>
            <end position="352"/>
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    </feature>
    <feature type="domain" description="KA1" evidence="21">
        <location>
            <begin position="722"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="13"/>
            <end position="40"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="357"/>
            <end position="509"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="563"/>
            <end position="675"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="361"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="476"/>
            <end position="498"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="611"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="643"/>
            <end position="673"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="771" mass="86038" checksum="DD229572AE3355EB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKP0</accession>
    <name>A0A673CKP0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Integral membrane protein GPR137B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115433796</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030011160.1">
        <property type="nucleotide sequence ID" value="XM_030155300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CKP0"/>
    <dbReference type="FunCoup" id="A0A673CKP0">
        <property type="interactions" value="600"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054467.1">
        <property type="protein sequence ID" value="ENSSORP00005053212.1"/>
        <property type="gene ID" value="ENSSORG00005023939.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433796"/>
    <dbReference type="InParanoid" id="A0A673CKP0"/>
    <dbReference type="OrthoDB" id="192544at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045779">
        <property type="term" value="P:negative regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029723">
        <property type="entry name" value="GPR137"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146:SF0">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR137B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="386" mass="43292" checksum="AE8B008A10C14C48" modified="2020-06-17" version="1">MAAPPDPPVPPPDPPVPPPTLGPAVRPFVTLGLTGVYTAFYALLFLFVYAQLWLVLRYRHKRVSFQTGFLFLCLVWAALRTLLFSFYFRNSRTVNSLGPVPFWSLYCLPVCLQFWTLSLMNLYCAQVVFKAKSKFDPELLRYRLPLYLLFLLVSLLFLVVNLACAVVVRTSSAETHAVVLVRVTVNDSLFVLCAVSLSVCLYKIAKMSLANIYLESKGTSVCQVTAIGAVVVLLYTSRACYNLVVLGLSNKRISSFDYDWYNVSDQADLQSTLGDAGYVVFGVVLFVWELLPTSLVVFFFRVRKPNLDRSGTGLPGHVFSSRAYFFDNPRRYDSDDDLAWSVMPPNIQASLASDSYEWGNQNGISAYIGGEDPFHLPLPPEELSHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKZ0</accession>
    <name>A0A673CKZ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Matrix metallopeptidase 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115410457</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CKZ0"/>
    <dbReference type="FunCoup" id="A0A673CKZ0">
        <property type="interactions" value="467"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057577.1">
        <property type="protein sequence ID" value="ENSSORP00005056281.1"/>
        <property type="gene ID" value="ENSSORG00005025050.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKZ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000012">
        <property type="entry name" value="Matrix metallopeptidase 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000103">
        <property type="entry name" value="Matrix metallopeptidase 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF323">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="18">
        <location>
            <begin position="135"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="17">
        <location>
            <begin position="359"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="17">
        <location>
            <begin position="416"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="16">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="14">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="15">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="545" mass="62836" checksum="6B4B8333FC03F85C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMM3</accession>
    <name>A0A673CMM3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Soluble calcium-activated nucleotidase 1</fullName>
            <ecNumber evidence="6">3.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Apyrase homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cant1b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ribonucleoside 5'-diphosphate + H2O = a ribonucleoside 5'-phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the apyrase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.6" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673CMM3"/>
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        <property type="protein sequence ID" value="ENSSORP00005053852.1"/>
        <property type="gene ID" value="ENSSORG00005024178.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CMM3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.100:FF:000001">
        <property type="entry name" value="Soluble calcium-activated nucleotidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.100">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009283">
        <property type="entry name" value="Apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036258">
        <property type="entry name" value="Apyrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13023">
        <property type="entry name" value="APYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13023:SF3">
        <property type="entry name" value="SOLUBLE CALCIUM-ACTIVATED NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06079">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101887">
        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
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        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR609283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="417" mass="47275" checksum="87942F87A543E288" modified="2020-06-17" version="1">MTSLRVAIRSLPLALASMTQAATSDSRFHPKWRAIAIATLLALVLMLYLQRTVVDRDDSSRGYYQNVGHSLKMHSDSKGHVFRDAYGQPTYRFSRHQKQEKPYNDTYPLSPPERTEHGIRYRIGVIADLDTASRSSKEQTWFSYMKRGYLTVSDSADRLEVEWDSNTVTLESHLAEKGRGMELSELVAFNGHLYSVDDRTGVVYRIEGSQAVPWVILPDGDGSVSKGFKAEWLAVKDDHLYVGGLGKEWTTTSGEVVNNNPEWVKVVGYHGDVEHQNWVPHYNALRNAAGIHPPGYLIHESAAWSERLQRWFFLPRRASHEHYEEIADERRATNLLLSCPADFSYITVRHVGPLNPTHGFSSFKFVPDTDDQIIVALKSEEDAGRIATYIIAFTLDGRVLMPETKIGDIKYEGLEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMV4</accession>
    <name>A0A673CMV4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear receptor-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nrbp2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May regulate apoptosis of neural progenitor cells during their differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029979206.1">
        <property type="nucleotide sequence ID" value="XM_030123346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CMV4"/>
    <dbReference type="Ensembl" id="ENSSORT00005056823.1">
        <property type="protein sequence ID" value="ENSSORP00005055540.1"/>
        <property type="gene ID" value="ENSSORG00005024789.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411275"/>
    <dbReference type="CTD" id="562626"/>
    <dbReference type="InParanoid" id="A0A673CMV4"/>
    <dbReference type="OrthoDB" id="1034557at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000098">
        <property type="entry name" value="Nuclear receptor-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000266">
        <property type="entry name" value="nuclear receptor-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="35"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00055098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="505" mass="57532" checksum="6F9D7CF1750F98DB" modified="2020-06-17" version="1">MTMSVPERKSGSEGKEEESEDESEILEESPCGRWQKRKEQVSQGNVPGVESASLAMDTEEGVEVVWNEVLFSDKKVFKAQEEKIKEMFENLMQVEHPNIVKFHKYWLDMKESQARVIFITEYMSSGSLKQFLKKTKKNHKTMNVKAWKRWCTQILSALSYLHSCDPPIIHGNLTCDTIFIQHNGLIKIGSVWHRLFVNVFPGASVHGKSRQHRDEQRTLHFFAPEYGTGADDYAIDIFSFGICALEMAVLEIQANGDTAVSKEAIANAGQSLEDPLMREFTQSCLRHEAKLRPTAHDLLFHRVLFEVHSLKLLAAHCLINNQYLLPENCVEEKTKSFDPNAIMAEIKHEDRQGVQLKYSHVSPLELDKFLEDVKNGIYPLMNFASSRPHPVPRALSLSQEQVETVKTPTPEPQETETRKVVQMHCNLESNEEGTKTHLSLFLKMDDKLHRQLSCDILPTDTSKDLASELVHYAFINEEDSEKVAGFLEDAISRHRVRALVSGATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRY6</accession>
    <name>A0A673CRY6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Golgi associated, gamma adaptin ear containing, ARF binding protein 3a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gga3a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CRY6"/>
    <dbReference type="Ensembl" id="ENSSORT00005059734.1">
        <property type="protein sequence ID" value="ENSSORP00005058411.1"/>
        <property type="gene ID" value="ENSSORG00005025783.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CRY6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1230:FF:000001">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027422">
        <property type="entry name" value="GGA1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905:SF3">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905">
        <property type="entry name" value="GOLGI-LOCALIZED, GAMMA-ADAPTIN EAR CONTAINING, ARF BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GAT" evidence="9">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="8">
        <location>
            <begin position="430"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="353"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="559" mass="59251" checksum="BBEF4352FAFA53D2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSD4</accession>
    <name>A0A673CSD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Choline O-acetyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">chata</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>choline + acetyl-CoA = acetylcholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:18821"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15355"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.6" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A673CSD4"/>
    <dbReference type="FunCoup" id="A0A673CSD4">
        <property type="interactions" value="1271"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056734.1">
        <property type="protein sequence ID" value="ENSSORP00005055452.1"/>
        <property type="gene ID" value="ENSSORG00005024737.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSD4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004102">
        <property type="term" value="F:choline O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008292">
        <property type="term" value="P:acetylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000001">
        <property type="entry name" value="Carnitine O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000004">
        <property type="entry name" value="Choline O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF14">
        <property type="entry name" value="CHOLINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="12">
        <location>
            <begin position="43"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="623"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="630"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="681" mass="77567" checksum="E32A34D84A74D001" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CV22</accession>
    <name>A0A673CV22_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ppil3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="RefSeq" id="XP_029999410.1">
        <property type="nucleotide sequence ID" value="XM_030143550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CV22"/>
    <dbReference type="FunCoup" id="A0A673CV22">
        <property type="interactions" value="1105"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057126.1">
        <property type="protein sequence ID" value="ENSSORP00005055838.1"/>
        <property type="gene ID" value="ENSSORG00005024892.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425795"/>
    <dbReference type="CTD" id="53938"/>
    <dbReference type="InParanoid" id="A0A673CV22"/>
    <dbReference type="OrthoDB" id="271386at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01928">
        <property type="entry name" value="Cyclophilin_PPIL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000012">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="161" mass="17947" checksum="D155412132986F59" modified="2020-06-17" version="1">MAVTLHTDLGDIKIELFCERTPKTSENFLALCASGFYNGSIFHRNIKGFMVQTGDPTGTGKGGTSIWGRKFEDEFSEHLKHNVRGVVSMANNGPNTNGSQFFFTYAKQPHLDMKYTAFGKIIDGLETLDELEKLPVNEKTFRPLTDTRIKDVTIHANPFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CX69</accession>
    <name>A0A673CX69_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Family with sequence similarity 189 member A1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ENTREP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CX69"/>
    <dbReference type="Ensembl" id="ENSSORT00005059567.1">
        <property type="protein sequence ID" value="ENSSORP00005058244.1"/>
        <property type="gene ID" value="ENSSORG00005025732.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CX69"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030431">
        <property type="entry name" value="ENTREP1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615:SF6">
        <property type="entry name" value="PROTEIN ENTREP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615">
        <property type="entry name" value="PROTEIN FAM189A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="204" mass="21958" checksum="475CB0E3B2125178" modified="2020-06-17" version="1">MPVNALPRGGSSGVGVGGPGSLSPASLSRSLSRLREYRTRTRIMLALGVSQMVLGSLILAVSFAALALTTSPRVRHSCPFWAGFSVLLSGLIGVVSWKRPLSLVITFFMLLSAVCVMLNLAGSILSCQNAQLVNSLDDCQLLKFDSDGVCVCCELQQQSSSCNNLGETLKLNPLRDCNTIRLRLKVGWANVCLFTMENYNLKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CZR7</accession>
    <name>A0A673CZR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="17">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pole</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit POLE and the accessory subunits POLE2, POLE3 and POLE4. Interacts with RAD17 and TOPBP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="17"/>
    <dbReference type="FunCoup" id="A0A673CZR7">
        <property type="interactions" value="1384"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059117.1">
        <property type="protein sequence ID" value="ENSSORP00005057798.1"/>
        <property type="gene ID" value="ENSSORG00005025575.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CZR7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
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        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
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        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
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    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
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    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 17" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 17" id="KW-0408">Iron</keyword>
    <keyword evidence="13 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="19">
        <location>
            <begin position="1527"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1291"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1940"/>
            <end position="1978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="1304"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1948"/>
            <end position="1978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2296" mass="263611" checksum="4F56DB0CCF81313B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673D0D6</accession>
    <name>A0A673D0D6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein kinase C</fullName>
            <ecNumber evidence="7">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">pkn1a</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673D0D6"/>
    <dbReference type="Ensembl" id="ENSSORT00005059426.1">
        <property type="protein sequence ID" value="ENSSORP00005058103.1"/>
        <property type="gene ID" value="ENSSORG00005025554.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673D0D6"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11630">
        <property type="entry name" value="HR1_PKN1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11622">
        <property type="entry name" value="HR1_PKN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05589">
        <property type="entry name" value="STKc_PKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000001">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000002">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000003">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000058">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000038">
        <property type="entry name" value="serine/threonine-protein kinase N2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037317">
        <property type="entry name" value="PKN1_HR1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037313">
        <property type="entry name" value="PKN_HR1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17 23 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="31"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="120"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="200"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="612"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="28">
        <location>
            <begin position="872"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="890"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="26">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="916"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="641"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="939" mass="106245" checksum="C89922493C46A82D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673D0H9</accession>
    <name>A0A673D0H9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
            <shortName evidence="4">SDH assembly factor 2</shortName>
            <shortName evidence="4">SDHAF2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sdhaf2</name>
        <name evidence="4" type="synonym">PGL2</name>
        <name evidence="4" type="synonym">SDH5</name>
        <name evidence="4" type="synonym">SDHAF2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SDHA of the SDH catalytic dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with SDHA within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SDHAF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979426.1">
        <property type="nucleotide sequence ID" value="XM_030123566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673D0H9"/>
    <dbReference type="FunCoup" id="A0A673D0H9">
        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059501.1">
        <property type="protein sequence ID" value="ENSSORP00005058178.1"/>
        <property type="gene ID" value="ENSSORG00005025698.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411438"/>
    <dbReference type="CTD" id="54949"/>
    <dbReference type="InParanoid" id="A0A673D0H9"/>
    <dbReference type="OrthoDB" id="284292at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010719">
        <property type="term" value="P:negative regulation of epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="163" mass="18997" checksum="5F7357EB88266E5F" modified="2020-06-17" version="1">MLSSFVARRLVTGVCQAAWRPAVTGLVTVRGYRGEAPDDTRGDLIEIPLPPWEEKTGEPMDIKKRRLLYESRKRGMLENCILLSLFAKRYLNTMSETQLQQYDRLINEPSNDWDIYYWATEALPTPEVYDGEVMDMLKEFAKNRDQEQRLDAPSLDYLEKESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673D0I2</accession>
    <name>A0A673D0I2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Lysozyme g</fullName>
            <ecNumber evidence="3 9">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115421217</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 23 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="3 9"/>
    <dbReference type="AlphaFoldDB" id="A0A673D0I2"/>
    <dbReference type="FunCoup" id="A0A673D0I2">
        <property type="interactions" value="801"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059506.1">
        <property type="protein sequence ID" value="ENSSORP00005058183.1"/>
        <property type="gene ID" value="ENSSORG00005025702.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673D0I2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01021">
        <property type="entry name" value="GEWL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000026">
        <property type="entry name" value="Lysozyme g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002152">
        <property type="entry name" value="Glyco_hydro_23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31698">
        <property type="entry name" value="LYSOZYME G FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31698:SF8">
        <property type="entry name" value="LYSOZYME G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001065">
        <property type="entry name" value="Lysozyme_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00749">
        <property type="entry name" value="LYSOZYMEG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="6" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglycosylase SLT" evidence="11">
        <location>
            <begin position="50"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001065-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="189" mass="20584" checksum="338AACEE1342AC16" modified="2020-06-17" version="1">MGYADIMDVETSGASAQTARQDNLHYAGVEASHTMAKTDLERVRRYKSIINSVAGRRGIDPALIAGIMSRESRAGNALDSQGWGDHGNGFGLMQVDVNPRGGGHTARGAWNSEEHVDQATGILVDFIGKISRKFPNWSKEQKLKGGIAAYNMGDGNVHDYHNVDARTTGRDYSNDVVARAQWYKKNGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673D0L3</accession>
    <name>A0A673D0L3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Adrenoceptor alpha 2C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115438965</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673D0L3"/>
    <dbReference type="FunCoup" id="A0A673D0L3">
        <property type="interactions" value="352"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059541.1">
        <property type="protein sequence ID" value="ENSSORP00005058218.1"/>
        <property type="gene ID" value="ENSSORG00005025716.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673D0L3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051379">
        <property type="term" value="F:epinephrine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030168">
        <property type="term" value="P:platelet activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006940">
        <property type="term" value="P:regulation of smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000131">
        <property type="entry name" value="alpha-2A adrenergic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000100">
        <property type="entry name" value="alpha-2B adrenergic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002233">
        <property type="entry name" value="ADR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000735">
        <property type="entry name" value="ADRA2C_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF25">
        <property type="entry name" value="ALPHA-2C ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00560">
        <property type="entry name" value="ADRENRGCA2CR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="61"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="234"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="297"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="319"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="449" mass="50392" checksum="349E1B48CED7CA5B" modified="2020-06-17" version="1">KAYPSLCASFEMDAFNASGMDAFTVIHLNSSSWSLDSGYSLAAIAGIAALVSFLILFTVVGNILVVIAVLTSRALKAPQNLFLVSLATADILVATLVMPFSLANELMGYWYFGKVWCGIYLALDVLFCTSSIVHLCAISLDRYWSVTQAVEYNLKRTPKRVKCIILIVWLISAFISSPPLISIDSNNDTSSQPQCELNDDTWYILSSSIASFFAPCLIMILVYIRIYQVAKTRTRSLSEKKPRPDGVTQTENGLSKGTPPFHSERENGHCQCPPTPSQRTGTVGQATEDADLEESSSSEGKGQHQPHPQQQHSEDTKRTKSQKKNSLSKHSARISRVSNKSIDLFASRRKRRRSSLARKKVSQAREKRFTFVLAVVMGVFVVCWFPFFFSYSLYGVCRDSCKIPDPLFKFFFWIGYCNSSLNPAIYTIFNRDFRHAFQKILCKSWKKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3R5</accession>
    <name>A0A672Y3R5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gpr146</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029996916.1">
        <property type="nucleotide sequence ID" value="XM_030141056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y3R5"/>
    <dbReference type="FunCoup" id="A0A672Y3R5">
        <property type="interactions" value="1048"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000483.1">
        <property type="protein sequence ID" value="ENSSORP00005000458.1"/>
        <property type="gene ID" value="ENSSORG00005000318.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423996"/>
    <dbReference type="CTD" id="115330"/>
    <dbReference type="InParanoid" id="A0A672Y3R5"/>
    <dbReference type="OrthoDB" id="8660770at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="7tmA_GPR146"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="GPER1-like"/>
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        <property type="entry name" value="GPR146"/>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 146-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182 AND ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="296"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="333" mass="38112" checksum="B8645AEC97B9340E" modified="2020-06-17" version="1">MWICMVYNETDASVDYQLCQDFGLILSVFSLIYLLVCFPLGLCYNVLLVVVNLSNKVSMTMPDVYFVNMAIAGLVLNVVAPVELLSSSFTRWFVWEYNNEVYITLLILFNISSLVIMYSTTLLSLDYYIERALPRTYMSSVYNTKHVCGFIWGGAVLTSFSSLLFYVCNHVSTKMIECSKMQNKEAADAIMMFIGYVVPAVAVLYAFVLILRIRKESTPLDQDSARLDPSIHRLLLASVCVQFVLWTPYYMTLLVHTIAGTPGYISNGHHIPTYYFLRCVSKLLAFSSSFAMPLMYRQMNKNFSNKLQRLLRRLHCRDQSCPHERSTVQQVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y435</accession>
    <name>A0A672Y435_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Calcium voltage-gated channel auxiliary subunit alpha2delta 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel subunit alpha-2/delta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y435"/>
    <dbReference type="Ensembl" id="ENSSORT00005000684.1">
        <property type="protein sequence ID" value="ENSSORP00005000658.1"/>
        <property type="gene ID" value="ENSSORG00005000426.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y435"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="vWA_VGCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium channel, voltage-dependent, alpha2/delta subunit 3"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000007">
        <property type="entry name" value="Calcium voltage-gated channel auxiliary subunit alpha2delta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF59">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="715"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="207"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007060"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="933" mass="106569" checksum="95E90A723B40B2A6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4V6</accession>
    <name>A0A672Y4V6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18 19">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 19">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">hacd3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Involved in Rac1-signaling pathways leading to the modulation of gene expression.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(3R)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:39159"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:74278"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:39160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:45813"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Endoplasmic reticulum membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 19"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y4V6"/>
    <dbReference type="Ensembl" id="ENSSORT00005001147.1">
        <property type="protein sequence ID" value="ENSSORP00005001113.1"/>
        <property type="gene ID" value="ENSSORG00005000667.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4V6"/>
    <dbReference type="OrthoDB" id="2157530at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06465">
        <property type="entry name" value="p23_hB-ind1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000016">
        <property type="entry name" value="Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF20">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 19" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8 19" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 19" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="20">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="353" mass="41653" checksum="4AB00CAB7ED80243" modified="2020-06-17" version="1">MALTPHVYWAQRHDDIYLRVELTDAQNISVKVHENVLRFRAQGRGAKGQNEYEFSLEFLLPVKPEISYKSTQRQVNITLQKEQRGWWEKLTVHERKPVFLAPDFDRWLDESDAEMEIQEREEKLKAKHGPDNAFDNPKLGFLFSYNLVQFLGFSWIFINMTVRLFIFGQDFLYDTFHSISKVMFFCQILASVEVLNAAFGVVRTRVLPSLIQVVGRNFILFIIFGSLEEMQNKPVVFFVFYLWSSIEIIRYPFYLLSYCDIEWKTLTWLRYTVWIPLYPLGVLAEAVAVIQAIPMFDETKLFSIPLPKPIGTSVSFSYILYIYLVLMVLGLYINFCHLYKLRMRRIHTKKKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y575</accession>
    <name>A0A672Y575_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor jun-B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115424341</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Jun subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997409.1">
        <property type="nucleotide sequence ID" value="XM_030141549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y575"/>
    <dbReference type="Ensembl" id="ENSSORT00005001625.1">
        <property type="protein sequence ID" value="ENSSORP00005001577.1"/>
        <property type="gene ID" value="ENSSORG00005000989.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424341"/>
    <dbReference type="InParanoid" id="A0A672Y575"/>
    <dbReference type="OrthoDB" id="2187714at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14696">
        <property type="entry name" value="bZIP_Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000012">
        <property type="entry name" value="Putative transcription factor AP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005643">
        <property type="entry name" value="JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF58">
        <property type="entry name" value="JUN B PROTO-ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03957">
        <property type="entry name" value="Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="251"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="249"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="330" mass="36273" checksum="DFB4B9116729A65E" modified="2020-06-17" version="1">MSTIMEQPFYDDSFLSAYGHPGAALPDYKLLKQNMNLNFSESYRNSNFKSQHLRADSDFYPAGAADVGSLKLASPELERLIIQNSNGVITTTPTPGQYIYNRGITEEQEGFAEGFVKALDDLHKMNQMAPPNVSIGTGGVACSAPASVFGSSMQPEPLEYTTLSSCTTNHSLSSAASYPSTTISYLPHQYHQHPQAIAHGSHHFQQSVAGTGIHTQRFGGLKEEPQTVPDMQSSDNGSPPMSPIDLENQERIKAERKRLRNRLAASKCRRRKLERIARLEDKVKVLKTDNAGLSSTASVLREQVAQLKQKVMTHVSSGCQLMLAPKVKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y7F1</accession>
    <name>A0A672Y7F1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Vacuolar-sorting protein SNF8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">snf8</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SNF8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029977760.1">
        <property type="nucleotide sequence ID" value="XM_030121900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y7F1"/>
    <dbReference type="FunCoup" id="A0A672Y7F1">
        <property type="interactions" value="1687"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002653.1">
        <property type="protein sequence ID" value="ENSSORP00005002587.1"/>
        <property type="gene ID" value="ENSSORG00005001555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410300"/>
    <dbReference type="CTD" id="11267"/>
    <dbReference type="InParanoid" id="A0A672Y7F1"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000085">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000234">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017215">
        <property type="entry name" value="ESCRT2_Vps22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="258" mass="28912" checksum="AE00543446543CBF" modified="2020-06-17" version="1">MHRRGVGAGAIAKKKLAEAKYKERGTVLAEDQIVQMSKQLETFKSHLEEFASKHKQEIRKNSQFRVQFQEMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLDELHQRVLKGRGKYAQDVSQDDLVRAIKKLKVMGNGFGMIPVGGSYLVQSVPAELNMDHTVVLQLAEKKGYVTVSEIKDSLKWEKERASHVLDHLLKEGLAWLDSQAAGEPQYWLPALFSELTSRDITPEEANQMTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8I1</accession>
    <name>A0A672Y8I1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="10">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">usp37</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="10"/>
    <dbReference type="RefSeq" id="XP_029980667.1">
        <property type="nucleotide sequence ID" value="XM_030124807.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029980668.1">
        <property type="nucleotide sequence ID" value="XM_030124808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y8I1"/>
    <dbReference type="FunCoup" id="A0A672Y8I1">
        <property type="interactions" value="722"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003137.1">
        <property type="protein sequence ID" value="ENSSORP00005003043.1"/>
        <property type="gene ID" value="ENSSORG00005001882.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412354"/>
    <dbReference type="CTD" id="57695"/>
    <dbReference type="InParanoid" id="A0A672Y8I1"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000040">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000066">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.180">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 26/29/37, pleckstrin homology-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032069">
        <property type="entry name" value="USP37-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038093">
        <property type="entry name" value="USP37-like_PH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF915">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16674">
        <property type="entry name" value="UCH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="13">
        <location>
            <begin position="312"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="108"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="606"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="691"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="765"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="947" mass="105940" checksum="09F58E72C02EA537" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YE46</accession>
    <name>A0A672YE46_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115430775</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YE46"/>
    <dbReference type="Ensembl" id="ENSSORT00005001474.1">
        <property type="protein sequence ID" value="ENSSORP00005001431.1"/>
        <property type="gene ID" value="ENSSORG00005000875.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YE46"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000017">
        <property type="entry name" value="KN motif and ankyrin repeat domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047184">
        <property type="entry name" value="KANK1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168:SF19">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="192"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="298" mass="31864" checksum="DF9AB1A8010D272B" modified="2020-06-17" version="1">MQKKGASNSRELPQEGAQARKGRSGTNKGPKGSGISHTPQRFKFSEKMLSACQALKMHLVDDKSLSSRDLHDSLQTLQQEWFFVSSQKAAVPDTVEDYLSAFRVISPSVLQHIANMADGNGNTALHYSVSHSNFGVVKKLLDAEVCNVNQQNKAGYTPIMLAALAAVDSPEDMRVVKELFTKGDVNAKASQAGQTALMLAVSHGRMDMVQALLAQGAQVNLQDDEGSTALMCASEHGHVDIVKLLLVQPDCDATLTDSDESTALSIALEAGHNEIAMLLYAHANFSKGQTGVSGSSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YER0</accession>
    <name>A0A672YER0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">General transcription factor IIF subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Transcription initiation factor IIF subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gtf2f2a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YER0"/>
    <dbReference type="FunCoup" id="A0A672YER0">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003028.1">
        <property type="protein sequence ID" value="ENSSORP00005002940.1"/>
        <property type="gene ID" value="ENSSORG00005001776.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YER0"/>
    <dbReference type="OrthoDB" id="26094at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015849">
        <property type="entry name" value="TFIIF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="12">
        <location>
            <begin position="16"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="11">
        <location>
            <begin position="194"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="268" mass="30257" checksum="B6D7E57089931ABF" modified="2020-06-17" version="1">MSEKGELDLTGVKQNTGVWLVKVPKYLSQQWAKATGRGEVGKIRICKKGNQGKAEVSFTLNEELTVIEGIDDKTVSAPREHPFTMQTVGGQTLAVYTETSSGQSEERSDGSSSGSGAGPDKIALEGMVVQRAECRPAVSESYMRLKRLQIEESSKPIRLSQQLDKAVTSNYKPVANHANNLEYERKKKEEGKRARAEKQQVLDMLFSAFEKHQYYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHYQGEEKTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFF2</accession>
    <name>A0A672YFF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YFF2"/>
    <dbReference type="Ensembl" id="ENSSORT00005002711.1">
        <property type="protein sequence ID" value="ENSSORP00005002636.1"/>
        <property type="gene ID" value="ENSSORG00005001616.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFF2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="48"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="161" mass="18542" checksum="0559C2643855318C" modified="2020-06-17" version="1">MSNISYEYDDESLVEMCFCLQIFLVVSCQLFCTMEIFGFGWRMSFCSCYFFSSTAGPWEAGRNDCKNRDADLVVIDSTEEQFIQGFKDYYWIGLTDRVNEGTWKWVDGSPVTLTFWDKGEPNNGMGSNGVNHGEEDCAEKPAKGKWNDLSCNTLRRWICEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHZ6</accession>
    <name>A0A672YHZ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein heavy chain hydrolytic ATP-binding dynein motor region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YHZ6"/>
    <dbReference type="Ensembl" id="ENSSORT00005004311.1">
        <property type="protein sequence ID" value="ENSSORP00005004186.1"/>
        <property type="gene ID" value="ENSSORG00005002547.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YHZ6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF66">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025584827" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="3">
        <location>
            <begin position="11"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="217" mass="23654" checksum="47AD4915B95C62E7" modified="2020-06-17" version="1">SFLFVVAFFPFRTLFGALHLHLGGAPEGPAGTGKTETTKDLAKAVAKQCVVFNCSDGLDYIALGKFFKGLLSCGAWACFDEFNRIDLEVLSVVAQQILTIQRGISTHMEVLVFEGTELKLDPSCAVFITMNPGYAGRSELPDNLKALFRTVAMMVPDYAMIAEIMLYSCGFVTARPLSVKIVATYRLCSEQLSSQPHYDYGMRAVKSVLTAAGNLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKB4</accession>
    <name>A0A672YKB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">stearoyl-CoA 9-desaturase</fullName>
            <ecNumber evidence="4">1.14.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115410413</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>octadecanoyl-CoA + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (9Z)-octadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:19721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="EC" id="1.14.19.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="17">The histidine box domains are involved in binding the catalytic metal ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029977919.1">
        <property type="nucleotide sequence ID" value="XM_030122059.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029977921.1">
        <property type="nucleotide sequence ID" value="XM_030122061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YKB4"/>
    <dbReference type="FunCoup" id="A0A672YKB4">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005158.1">
        <property type="protein sequence ID" value="ENSSORP00005005014.1"/>
        <property type="gene ID" value="ENSSORG00005002978.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410413"/>
    <dbReference type="InParanoid" id="A0A672YKB4"/>
    <dbReference type="OrthoDB" id="10260134at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032896">
        <property type="term" value="F:palmitoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004768">
        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903966">
        <property type="term" value="P:monounsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070542">
        <property type="term" value="P:response to fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03505">
        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001522">
        <property type="entry name" value="FADS-1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF102">
        <property type="entry name" value="STEAROYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00476">
        <property type="entry name" value="FATTY_ACID_DESATUR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15 17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="20">
        <location>
            <begin position="75"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="333" mass="38431" checksum="C775F472D873A36D" modified="2020-06-17" version="1">MTEAETVEKEHKSSNGSNVPPEATREDAFDSSYKEKEGPKPPRIIVWRNVFLMLLLHIGAAYGIFLIPTASPLTLLWSLFCFVLSALGITAGAHRLWSHRSYKASLPLRIFLCIANSMAFQNDIFEWARDHRVHHKYSETDADPHNAVRGFFFAHIGWLLVRKHPDVITKGRKLELKDLMADKVVMFQRKYYKLSVLLMCFFIPMSVPWYLWGESLWVGYFIPAVLRYTLVLNATWLVNSAAHMWGNRPYDKNISPRENRFVTFSAIGEGFHNYHHTFPYDYATSEFGCKLNLTTAFIDFMCFLGLAKDRKRVSREQVLARKQRTGDGSHRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNW9</accession>
    <name>A0A672YNW9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C-Jun-amino-terminal kinase-interacting protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">JNK MAP kinase scaffold protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">mapk8ip3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the JIP scaffold family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005006212.1"/>
        <property type="gene ID" value="ENSSORG00005003513.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YNW9"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:dendrite"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:MAP-kinase scaffold activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:signaling receptor complex adaptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
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        <property type="entry name" value="C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JNK/SAPK-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JIP_LZII"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
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        <location>
            <begin position="510"/>
            <end position="581"/>
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        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <end position="318"/>
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            <end position="511"/>
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        <location>
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        <location>
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            <begin position="180"/>
            <end position="190"/>
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            <begin position="247"/>
            <end position="270"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51776"/>
        </source>
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            <dbReference type="PROSITE" id="PS51777"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006212.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1304" mass="144717" checksum="A85A240518C58E72" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YP96</accession>
    <name>A0A672YP96_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="476" mass="53955" checksum="E9B41149943150DF" modified="2020-06-17" version="1">MGTRMEVAQLESRMSTEDYRKLQSASRSLSRGEFIDLAWSSVGHGSKEEYGLLFDNSDAVKEEGLVDWAGLCSFLVLQLSDKVKNTRVSRVPCWKPPRTLTYPHRDPLQKVLYLQSSGQYLTVSKGGTVGLRDGEDMSLVHTHRLQNSTVRPKDLWVTDMVLLHNVQKVAVSFTSKEVCFYDIISKQDFICKYKLQGLKFTPWCLDYWVDPSHPDQAILTIGDTGGQVCVLYFTSAHICLFERLSLRTDSDSADIVLWDELTKGKHRCCCTVTHQAHEPAWVRRVRYLASLDAFVSCSTSPHSSVVIGWRGKESKNLRITSCFTKRGVWDVDHHHELNLIATAGADHQVLLWNPYMTSKPVGVLSGHDGPVIAVCFMQNKQQLLSYSKDKVLCLWDVSTEQCVQRLAGVFPNTQEGTRTLLFLHEESQLLLISFNSLLLLLETRQEEKRTNSHEHPVTCVLYNGLFRQGEDDGPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPK1</accession>
    <name>A0A672YPK1_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPK1"/>
    <dbReference type="Ensembl" id="ENSSORT00005006744.1">
        <property type="protein sequence ID" value="ENSSORP00005006488.1"/>
        <property type="gene ID" value="ENSSORG00005003807.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPK1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="245" mass="27360" checksum="E9C0BFC6A98289A4" modified="2020-06-17" version="1">PIKTQTSTIDQKRDRINQSLSELRTLLLNSTCDPRLQNPKIEKAEILDLTVEYLQKWTKGTNEACSSSLFTIEGAGFQQCMARLSSYMHKITPEQRTHLMEGLKHHTENQQQRPHTSSSAAAFELTLSELPPTGSERKEEPPKLLFLPPSCSTPRHDYLSPPPSPWFSPLSTYATSPTFPSFACHFSFPPTSLSPPSAHNATSSTLPQPSHAIHTPTPALHLHPPRHPTQGEVSPPSSSIWRPWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQS9</accession>
    <name>A0A672YQS9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Caspase-6</fullName>
            <ecNumber evidence="14">3.4.22.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115429459</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.</text>
            <dbReference type="EC" id="3.4.22.59"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (Caspase-6 subunit p18) and a 11 kDa (Caspase-6 subunit p11) subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.59" evidence="14"/>
    <dbReference type="RefSeq" id="XP_030004770.1">
        <property type="nucleotide sequence ID" value="XM_030148910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YQS9"/>
    <dbReference type="Ensembl" id="ENSSORT00005007211.1">
        <property type="protein sequence ID" value="ENSSORP00005006949.1"/>
        <property type="gene ID" value="ENSSORG00005004009.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429459"/>
    <dbReference type="InParanoid" id="A0A672YQS9"/>
    <dbReference type="OrthoDB" id="6116485at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000001">
        <property type="entry name" value="Caspase-3 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454">
        <property type="entry name" value="CASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454:SF206">
        <property type="entry name" value="CASPASE-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Caspase family p20" evidence="18">
        <location>
            <begin position="55"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="17">
        <location>
            <begin position="205"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="301" mass="34686" checksum="6B970823BE1F86D2" modified="2020-06-17" version="1">MSDATGEDICGGNVVRSSTTTAASTVFSVNLTETEACKSDLFLDLEAEYNMTNKRRGLALIFNYEYFFWQRRIPPRDGTTVDRFKLVTRLRDLNFEVRAYDNYRKQEVFDKLHEAAEADHSDADCFLLVFLSHGKDDHVEAFDDEISIQHMTSLFREDKCRTLSGKPKIFILQACRGDKHDDPVTPCDAVDSELEVEEVVEEAAGVHMSPAWDDFIMCYSVAEGYYSHRNNRYGSWYIQDLCELLQKYGESLEFTELLTLVNRKVSMRSVDKCRDQDAIGKKQVPCFASMLTKKLYFRPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSL9</accession>
    <name>A0A672YSL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YSL9"/>
    <dbReference type="Ensembl" id="ENSSORT00005007777.1">
        <property type="protein sequence ID" value="ENSSORP00005007497.1"/>
        <property type="gene ID" value="ENSSORG00005004268.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YSL9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="119" mass="12914" checksum="16052AC9F9283838" modified="2020-06-17" version="1">MTLLYVCVSVSSESRGQVTVTQPGAVSSDLGASTTITCTTSRNVESSNLLSWYQQRDGETPKLLIYYATTRQSGISSRFSGSGSNSHFTLIISDVQAEDAAVYYCQSLHIINSQNVFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTT0</accession>
    <name>A0A672YTT0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YTT0"/>
    <dbReference type="Ensembl" id="ENSSORT00005008262.1">
        <property type="protein sequence ID" value="ENSSORP00005007979.1"/>
        <property type="gene ID" value="ENSSORG00005004468.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTT0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="131" mass="15338" checksum="D79843A50D130620" modified="2020-06-17" version="1">LTYYNYLHLTCFHMLVNIGAQASHHTDSYANSDGDIKIWPVQYLIYVEQNRGIVITLLCLFICLFATLWENYSNHLYQIVPTDRPRPWDEPITFWVTKSTIFLSLIIEQFSKIHKKCKTTPISLQFDPPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YV50</accession>
    <name>A0A672YV50_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NACHT, LRR and PYD domains-containing protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115433948</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030011402.1">
        <property type="nucleotide sequence ID" value="XM_030155542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YV50"/>
    <dbReference type="Ensembl" id="ENSSORT00005008831.1">
        <property type="protein sequence ID" value="ENSSORP00005008531.1"/>
        <property type="gene ID" value="ENSSORG00005004737.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433948"/>
    <dbReference type="InParanoid" id="A0A672YV50"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="9">
        <location>
            <begin position="286"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="965"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1160" mass="131314" checksum="CA0894EE1FB3E9DD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZQ4</accession>
    <name>A0A672YZQ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Collagen alpha-1(XXVIII) chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May act as a cell-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the VWA-containing collagen family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZQ4"/>
    <dbReference type="Ensembl" id="ENSSORT00005010348.1">
        <property type="protein sequence ID" value="ENSSORP00005010018.1"/>
        <property type="gene ID" value="ENSSORG00005005459.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZQ4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22628">
        <property type="entry name" value="Kunitz_collagen_alpha1_XXVIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01472">
        <property type="entry name" value="vWA_collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000051">
        <property type="entry name" value="Collagen type XXVIII alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF87">
        <property type="entry name" value="COLLAGEN ALPHA-1(VI) CHAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0084">Basement membrane</keyword>
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</entry>
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    <accession>A0A672Z027</accession>
    <name>A0A672Z027_9TELE</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            </authorList>
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        <text evidence="1">Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <text evidence="1">Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
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<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
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            <taxon>Neoteleostei</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1435" mass="151128" checksum="967396F03D2226C4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2B7</accession>
    <name>A0A672Z2B7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kit ligand</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Mast cell growth factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Stem cell factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">c-Kit ligand</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">kitlga</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SCF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z2B7"/>
    <dbReference type="FunCoup" id="A0A672Z2B7">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011277.1">
        <property type="protein sequence ID" value="ENSSORP00005010903.1"/>
        <property type="gene ID" value="ENSSORG00005005904.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2B7"/>
    <dbReference type="OrthoDB" id="8445223at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of cell population proliferation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11574:SF0">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0339">Growth factor</keyword>
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    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="12 25" id="KW-0732">Signal</keyword>
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    <keyword evidence="14 24" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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            <begin position="25"/>
            <end position="269"/>
        </location>
    </feature>
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        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="269" mass="31178" checksum="9B827EC5F9156AA1" modified="2020-06-17" version="1">MKKSKIWIRLCVHLLLFITLGVHSSKFDVNPVTDDISKLSILRQNIPKDYRIPVHFVPREEGGMCWVKLNVFYLEESLQDLAHKFGNISSNRKDISIFIQMLQELRLNMGSLESIMYDFQCHYREERWPTARYFDFVKDFLIAAQHKEDSDDCDPPPCPTTEEMITTEEYLTGSTMSSSKGTTCDLQRNCQTQTRFMSEVVERSLLSLLFIPLLAIIFLLVWKLRSRRNEEDLQPNPGEDLFFTATEGTAPPLDAEISEKNKLNVIETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3N9</accession>
    <name>A0A672Z3N9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Apogonoidei</taxon>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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            <dbReference type="Ensembl" id="ENSSORP00005011861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="184" mass="20939" checksum="7FE13E871FF9C2C2" modified="2020-06-17" version="1">MLFLGNREPQWRQVRYVLRAGHFLEGVGKSFPLKNPHGAKWILGGEDDTIYKGKDAEVNGWGKFYLPKQVKMKVIGVIEGTSCPNEQLVLMICEDGAFYAYDGEELHAVASNLDHLLNKGIEYPAAKSYYKGEAFKDMQWAEVRKGAVGKRLEEEHRKLVTANKSSFLEILKSTKQHKGQYLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3V8</accession>
    <name>A0A672Z3V8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Olfactory receptor C family, v3</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
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            <taxon>Sphaeramia</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Taste 1 receptor member 2"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF504">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR ISOFORM X2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="517"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="593"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="637"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="682"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="749"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="12">
        <location>
            <begin position="523"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="810" mass="90164" checksum="43EE50368A3030B6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4K2</accession>
    <name>A0A672Z4K2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Digestive cysteine proteinase 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">zgc:110239</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z4K2"/>
    <dbReference type="FunCoup" id="A0A672Z4K2">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012023.1">
        <property type="protein sequence ID" value="ENSSORP00005011638.1"/>
        <property type="gene ID" value="ENSSORG00005006214.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z4K2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000087">
        <property type="entry name" value="Counting factor associated protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="8">
        <location>
            <begin position="247"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="7">
        <location>
            <begin position="329"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="548" mass="61142" checksum="2BC1E154C48B376E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5C2</accession>
    <name>A0A672Z5C2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="15">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">plk1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z5C2"/>
    <dbReference type="Ensembl" id="ENSSORT00005012437.1">
        <property type="protein sequence ID" value="ENSSORP00005012045.1"/>
        <property type="gene ID" value="ENSSORG00005006384.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z5C2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000727">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000284">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF93">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="42"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="18">
        <location>
            <begin position="397"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="18">
        <location>
            <begin position="497"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="587" mass="66885" checksum="F664BFB099713B8E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5N7</accession>
    <name>A0A672Z5N7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">bckdk</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_030009702.1">
        <property type="nucleotide sequence ID" value="XM_030153842.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012173.1">
        <property type="protein sequence ID" value="ENSSORP00005011787.1"/>
        <property type="gene ID" value="ENSSORG00005006111.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432829"/>
    <dbReference type="CTD" id="10295"/>
    <dbReference type="InParanoid" id="A0A672Z5N7"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial matrix"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF39">
        <property type="entry name" value="PROTEIN-SERINE_THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="286"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="428" mass="47932" checksum="08192828423851EA" modified="2020-06-17" version="1">MRPGLAGIAVEMLSGAACRARPRLGCLLLSPVTKTVTPMSCTGSHRLRSTSSMQGFSELARERSKTVTSFYNQSAIDASAEKASVRLTLATLLYSGKSPDGHHILSSAKYLHKELPVRIAHRIKGFRSLPFIIGCNPTILQVHELYIRAYHMLVDFPPIKDQDVEARFCKLVQQLLDDHKDVVTMLAQGFRECRKHVQDETILRNFLDTTLCSRLGIRMLATHHLALHEDNPDFVGIICRRLSPKKIIEKWVDFARRLCEHQYGNAPRVRINGHVAARFPFIPLPLDYVLPELLKNAMRATMESHLDTPYNVPDVVVTIANNDIDFVIRISDRGGGIPHNIVDKVMDYHFSTAEESAQDPRMSNLFNNITNSGNQSSPMHGFGFGLPTSRAYAEYLGGSLSIQSMQGIGTDVYLRLRHIDGKGESFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCJ8</accession>
    <name>A0A672ZCJ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hoxa9b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZCJ8"/>
    <dbReference type="FunCoup" id="A0A672ZCJ8">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014635.1">
        <property type="protein sequence ID" value="ENSSORP00005014213.1"/>
        <property type="gene ID" value="ENSSORG00005007281.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZCJ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:proximal/distal pattern formation"/>
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        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A9"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="187"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014213.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="255" mass="28920" checksum="ABB287DB4D7C2654" modified="2025-10-08" version="2">MSRRRGKGQTSYYVDSRVLPEQEDMAPRYSSAAGVEQAMLSEYGGQESCPLQTKSSIFGGSWSPISTQPPNTTAPTYLPHHYTSGDSDGMFTRSWALDPVSASLCLTGLPPTAMHYEIKPEPLIGSGECTTLETHTPLLSDIENGASLAEIPCETTTASKPTDESPSAEEKREIDANNPSSNWLHAKPTRKKRCPYTKHQILELEKEFLFNMYLPRDRRYEVARLLNLTERQVKIWFQNRRMKMKKENKDRRRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDH3</accession>
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        <submittedName>
            <fullName evidence="4">G patch domain containing 1</fullName>
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        <name evidence="4" type="primary">gpatch1</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the GPATCH1 family.</text>
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            <begin position="197"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00008600"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014985.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="728" mass="81366" checksum="33C1749B8A0B278E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFW9</accession>
    <name>A0A672ZFW9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nerve growth factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ngfb</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZFW9"/>
    <dbReference type="FunCoup" id="A0A672ZFW9">
        <property type="interactions" value="1006"/>
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    <dbReference type="Ensembl" id="ENSSORT00005016407.1">
        <property type="protein sequence ID" value="ENSSORP00005015910.1"/>
        <property type="gene ID" value="ENSSORG00005008041.1"/>
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    <dbReference type="InParanoid" id="A0A672ZFW9"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:synaptic vesicle"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005163">
        <property type="term" value="F:nerve growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021675">
        <property type="term" value="P:nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038180">
        <property type="term" value="P:nerve growth factor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neuron projection morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Brain-derived neurotrophic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020408">
        <property type="entry name" value="Nerve_growth_factor-like"/>
    </dbReference>
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        <property type="entry name" value="Nerve_growth_factor-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019846">
        <property type="entry name" value="Nerve_growth_factor_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11589:SF10">
        <property type="entry name" value="BETA-NERVE GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11589">
        <property type="entry name" value="NERVE GROWTH FACTOR NGF -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00243">
        <property type="entry name" value="NGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001789">
        <property type="entry name" value="NGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00268">
        <property type="entry name" value="NGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00140">
        <property type="entry name" value="NGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00248">
        <property type="entry name" value="NGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50270">
        <property type="entry name" value="NGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025574680" evidence="8">
        <location>
            <begin position="19"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Nerve growth factor-related" evidence="9">
        <location>
            <begin position="89"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="97"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="140"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="150"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001789-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="201" mass="22344" checksum="0914605EC2E5A4FC" modified="2020-06-17" version="1">MRSSMLALLLFFSAQAVATTGGDSCATRTTQQQDSDHNHNTVPTVDPKLFNKRRYRSPRVLFSAQPPDAEPAESQGGSRRTRRRAGGSLHRGMYSVCESISVWVGNKTKATDISGNEVTVLPDVNINNVNKKQYFFETTCHSSRSGGSGCLGIDAKHWNSYCTNSHTFVRALTSFKNLVAWRLIRINVACVCVLSRKSWRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJ60</accession>
    <name>A0A672ZJ60_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">VIP36-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lman2la</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZJ60"/>
    <dbReference type="Ensembl" id="ENSSORT00005017032.1">
        <property type="protein sequence ID" value="ENSSORP00005016522.1"/>
        <property type="gene ID" value="ENSSORG00005008377.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJ60"/>
    <dbReference type="OrthoDB" id="270293at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000017">
        <property type="entry name" value="Vesicular integral-membrane protein VIP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF20">
        <property type="entry name" value="VIP36-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="13">
        <location>
            <begin position="54"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="365" mass="42043" checksum="0F74E727EF260814" modified="2020-06-17" version="1">MAAASTSDNLTHSKSVFDVLLRRKNMPFKTYCTLVILIFTFGRCFADDDHEMEEFLKREYSLSKPYQGVGSLGSSHWELMGDAMVTTEQVRLTPDMQSRQGAVWSRIPCHLKDWEMQVHFKIHGQGKKNLNGDGLAIWYTKERMQKGPVFGNKDNFTGLGVFVDTYPNEEKHLEAQKKRYTPRTQRIFPFVLAMVGNGTISYDHERDGRPTELGGCNAMVRNLKHDTFLFIRYVRRRLTVMIDIDGQHEWRDCLDIPGVRLPQGYYFGASAITGDLSDNHDIISLKLYQLTVLRSPKEEEEEEEEITIPSVDNMELLRLGQTEEGMSGIAIFLTVLFSMLGCIFLIVIGLVVYSHWNESRRKRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZMI2</accession>
    <name>A0A672ZMI2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MAM domain containing 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mamdc2a</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZMI2"/>
    <dbReference type="FunCoup" id="A0A672ZMI2">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018550.1">
        <property type="protein sequence ID" value="ENSSORP00005018009.1"/>
        <property type="gene ID" value="ENSSORG00005008951.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZMI2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000128">
        <property type="entry name" value="enteropeptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051560">
        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282">
        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF101">
        <property type="entry name" value="MAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025541010" evidence="2">
        <location>
            <begin position="27"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="3">
        <location>
            <begin position="33"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="3">
        <location>
            <begin position="170"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="3">
        <location>
            <begin position="340"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="3">
        <location>
            <begin position="506"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="684" mass="77349" checksum="3DA845DCD356F1B5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQK4</accession>
    <name>A0A672ZQK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Wiskott-Aldrich syndrome protein family member</fullName>
            <shortName evidence="7">WASP family protein member</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">wasf3a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds actin and the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SCAR/WAVE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZQK4"/>
    <dbReference type="Ensembl" id="ENSSORT00005019216.1">
        <property type="protein sequence ID" value="ENSSORP00005018667.1"/>
        <property type="gene ID" value="ENSSORG00005009201.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQK4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000012">
        <property type="entry name" value="Wiskott-Aldrich syndrome protein family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028288">
        <property type="entry name" value="SCAR/WAVE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902:SF7">
        <property type="entry name" value="ACTIN-BINDING PROTEIN WASF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902">
        <property type="entry name" value="WASP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="9">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="214"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="481" mass="53739" checksum="319E7789905D9FB9" modified="2020-06-17" version="1">MPLVKRNIEPRHLCHGAVPDGIGNELECVTNNTLSAIIRQLSSLSKHAENVFGELFNEANTFYSRANSLQDRIDRLAIKVTQLDSSIEEVSLQDINMRKAFKSSTVQNQQVLSKGSTPNSVAEMYNSSDRPPPLSTLTAYREDSTDAMKFYSDPSYFFDLWKEKMLQDTEDKRKERRRQREQKRCVDSSTLQREVKKVRKVRNRRQEWNMMAFDKELRPDHRHPQTLRRGASSEGSLSPDGRLVSGSHDYVPGNAHPSPPVEHEYHSIDVNYKRVTYSTAEPHCTEPMNGAIRPPAEYKYVSHLHKQRAETVFTASTPVTFFCLYLSTSSIPPSSAPGPPIPSAQTAFGFPLGALPPPPPHNGVVHVGPGYPLPPVPPPGPRMVPPPPGPPPPPLPPPAAPPNLTSVSGRTENPPVRDARSDLLSAIRMGIQLKKVQEQQEQQSKWEPVGNDVATILSRRIAVEYSDSDDDSELDENEWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZ62</accession>
    <name>A0A672ZZ62_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">wdr82</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZZ62"/>
    <dbReference type="FunCoup" id="A0A672ZZ62">
        <property type="interactions" value="1988"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022424.1">
        <property type="protein sequence ID" value="ENSSORP00005021777.1"/>
        <property type="gene ID" value="ENSSORG00005010626.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZ62"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110064">
        <property type="term" value="P:lncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032785">
        <property type="term" value="P:negative regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140744">
        <property type="term" value="P:negative regulation of lncRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071027">
        <property type="term" value="P:nuclear RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11284">
        <property type="entry name" value="ADF_Twf-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11285">
        <property type="entry name" value="ADF_Twf-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000007">
        <property type="entry name" value="Twinfilin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000065">
        <property type="entry name" value="WD repeat-containing protein 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="7 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ADF-H" evidence="16">
        <location>
            <begin position="432"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="575"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="604" mass="69021" checksum="BE808A0B5DDC03FF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZX6</accession>
    <name>A0A672ZZX6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Membrane metallo-endopeptidase-like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZZX6"/>
    <dbReference type="Ensembl" id="ENSSORT00005023204.1">
        <property type="protein sequence ID" value="ENSSORP00005022539.1"/>
        <property type="gene ID" value="ENSSORG00005010967.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZX6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF141">
        <property type="entry name" value="MEMBRANE METALLO-ENDOPEPTIDASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="8">
        <location>
            <begin position="60"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="7">
        <location>
            <begin position="279"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="41278" checksum="BDC5DA4D7C8E1328" modified="2020-06-17" version="1">MKPQTKHKLVTLVSVIDCYHGNKDLSKPNNRVSNFLVLVCEIDQLALDLSKSKTENGLRSQTGFNWTRFIQGVLSSVSIDIQLEEEVVVYSSPYLEKMNDVLSRHSVRTMQNYLTWQLIIDRVNSLSRRFKDARARYRKTLYGTTVEDAWWRECVRYVQSSMENAVGALYVRETFAGESKRMVSDLISKIQKAYVETLEELSWMDTPSKEKAREKAMAIKEHIGDIPGLTCFSSQLNFSEEHYFENILENLKSEAHKSLKKLREPVDPDLWIIGAAVVNAFYSPNRNQIVFPAGILQPPFFSKHQHQALNFGGIGMVIGHEITHGFDDNGETVVIHCGYFGKPKANTVYHLESRYNNKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0A5</accession>
    <name>A0A673A0A5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KIAA1549-like b</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A0A5"/>
    <dbReference type="Ensembl" id="ENSSORT00005023339.1">
        <property type="protein sequence ID" value="ENSSORP00005022674.1"/>
        <property type="gene ID" value="ENSSORG00005011022.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A0A5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024606">
        <property type="entry name" value="KIAA1549"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21590">
        <property type="entry name" value="SEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21590:SF3">
        <property type="entry name" value="UPF0606 PROTEIN KIAA1549L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12877">
        <property type="entry name" value="KIAA1549"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="111" mass="12118" checksum="4E77C3D96A57187A" modified="2025-10-08" version="2">MLICVLFVGTGDDPRSCRFSQMMEQRLEKVFSEAQAKVFNTNGKLAVQMLSVSQAAGSPAVSLVYSVKNGTVFLNGTTASNLLGQLSAELVGYFLFYPPLIIAERKLQFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0S2</accession>
    <name>A0A673A0S2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">chst12</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_029989639.1">
        <property type="nucleotide sequence ID" value="XM_030133779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A0S2"/>
    <dbReference type="Ensembl" id="ENSSORT00005022839.1">
        <property type="protein sequence ID" value="ENSSORP00005022183.1"/>
        <property type="gene ID" value="ENSSORG00005010822.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419140"/>
    <dbReference type="InParanoid" id="A0A673A0S2"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF4">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="52"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="421" mass="48570" checksum="9CABD03ED88953F4" modified="2020-06-17" version="1">MGTCRGLRVAFLVGSLFMILLIIVYWDDVGGFNLYPKHELIHQISCPETTAGPPLLSSTSTAQPRSALTLPTAASSTTRVPEEDGGKDDIKEEGKEKQEQKQEETKKEEEEEALWSRTDAEDRKQEERKQRIMDVCSGKDEVEFLGRSRAFDHIPNGELDHLIVDDTHQIIYCYVPKVACTNWKRVLVVLSQSLISPSSGKPYTDPEAIPSEVVHNSTLHLTFAKFWRRYGSLSRHLMALKLQHYTKFLFVRDPFVRLISAFRNKFGRPNEDFYRQFGSVILRRYGNVSGSLPETAAEAFAAGITPTFQQFITYLLDPDTEKESIYNEHWRQVYRLCHPCQVKYDFIGRLESLETDAEHLLKTLKVDHLIRFPSGARNQTAVSWEREWFSQIPIAMRRKLYKLYEPDFKLFGYPKPDSVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A981</accession>
    <name>A0A673A981_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fasciculation and elongation protein zeta 1 (zygin I)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fez1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zygin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A981"/>
    <dbReference type="FunCoup" id="A0A673A981">
        <property type="interactions" value="667"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026609.1">
        <property type="protein sequence ID" value="ENSSORP00005025841.1"/>
        <property type="gene ID" value="ENSSORG00005012413.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A981"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011680">
        <property type="entry name" value="FEZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12394:SF4">
        <property type="entry name" value="FASCICULATION AND ELONGATION PROTEIN ZETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12394">
        <property type="entry name" value="ZYGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07763">
        <property type="entry name" value="FEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="406" mass="46210" checksum="FE8BD59120C511C7" modified="2020-06-17" version="1">MEAPLVCLDEEFEDLRPCRMDELDHEALNHSTYSSTNTTTIVPLAPITREDFSELENFSEMMSFKSMEDLVNEFDEKLNVCFHNYNTKTEGLAPIRNQSHNQEDEERLQDEDVWDALTDNYISTWDGPDSEGLNGNLSDQEIHEKEEEEMNEKNDNANCLNDEPLITADQVIEEIVEMMENSPDPGETEEEDEEESSHCSSRTNPSLLEEIRQLSQASNNNCSYEGLSLMPSSALVELLHRVEAAIREYSEELVSQLARRDELEFEKEVKNTFITALMEVQNRQKEQRDSSKRRRRDKALSLQGGTTSVTGGNTGSMGRTEKTGTMPVKRFSMEGLSNILQTGIRQTFGSTGNEKQYLNTVIPYEKKGTPPTVDDLQMLTKILYAIKEDSDKVPTLLTDYILKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AC01</accession>
    <name>A0A673AC01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 98</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tmem98</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM98 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029996798.1">
        <property type="nucleotide sequence ID" value="XM_030140938.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029996799.1">
        <property type="nucleotide sequence ID" value="XM_030140939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AC01"/>
    <dbReference type="FunCoup" id="A0A673AC01">
        <property type="interactions" value="1313"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027885.1">
        <property type="protein sequence ID" value="ENSSORP00005027100.1"/>
        <property type="gene ID" value="ENSSORG00005012959.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423912"/>
    <dbReference type="CTD" id="26022"/>
    <dbReference type="InParanoid" id="A0A673AC01"/>
    <dbReference type="OrthoDB" id="5978425at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000003">
        <property type="entry name" value="Transmembrane protein 98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029668">
        <property type="entry name" value="TMEM98"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32510">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32510:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="224" mass="24447" checksum="BA3376497AB490F5" modified="2020-06-17" version="1">METVVIVAIGVLATIFLASFVALVVVCRHRYCHPHDLLHHFDSKPTVDLIGAMETQSEPSELELDDVVITNPHIEAILENEDWIEDASGLVSHCISILKICHTLTEKLVAMTMGSGAKVKAPASLSDIITVAKRISPRVDDVVRSMYPPLDPILLDARATALLLSVSHLVLVTRNACHMSGSMDWIDQSLHAAEDHMVVLREAALASEPERSLPGADVQREQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADN6</accession>
    <name>A0A673ADN6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps23</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ADN6"/>
    <dbReference type="FunCoup" id="A0A673ADN6">
        <property type="interactions" value="1409"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028198.1">
        <property type="protein sequence ID" value="ENSSORP00005027411.1"/>
        <property type="gene ID" value="ENSSORG00005013083.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ADN6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="159" mass="17650" checksum="D70338BB588075D2" modified="2020-06-17" version="1">CEILVIIQFVSCLPYLPGKCRGLRTARKLRNHRREQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AE66</accession>
    <name>A0A673AE66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">slc25a5</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AE66"/>
    <dbReference type="Ensembl" id="ENSSORT00005027812.1">
        <property type="protein sequence ID" value="ENSSORP00005027028.1"/>
        <property type="gene ID" value="ENSSORG00005012935.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AE66"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000002">
        <property type="entry name" value="Putative ADP/ATP translocase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="6"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="111"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="212"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="303" mass="33437" checksum="51FCD331EA0B70AA" modified="2020-06-17" version="1">MSEQAISFAKDFLAGGIAAAISKTAVAPIERVKLLLQVQHASKQISADKHYKGIIDCIVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKKIFLDGVDKHTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGASEREFKGLGDCLVKISKSDGIRGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIVVSWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGADIMYTGTLDCWRKIARDEGSKAFFKGAWSNVLRGMGGAFVLVLYDEKPFGTSSCEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AES4</accession>
    <name>A0A673AES4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3bl</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sema3bl</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AES4"/>
    <dbReference type="FunCoup" id="A0A673AES4">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028577.1">
        <property type="protein sequence ID" value="ENSSORP00005027781.1"/>
        <property type="gene ID" value="ENSSORG00005013205.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AES4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF20">
        <property type="entry name" value="SEMAPHORIN-3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sema" evidence="13">
        <location>
            <begin position="27"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="535"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="725"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="725"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="759" mass="86225" checksum="977262A3800BE087" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIJ2</accession>
    <name>A0A673AIJ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heat shock cognate 70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115434996</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AIJ2"/>
    <dbReference type="FunCoup" id="A0A673AIJ2">
        <property type="interactions" value="1306"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030373.1">
        <property type="protein sequence ID" value="ENSSORP00005029540.1"/>
        <property type="gene ID" value="ENSSORG00005014013.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AIJ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000135">
        <property type="entry name" value="Heat shock cognate 71 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000134">
        <property type="entry name" value="Heat shock cognate 71 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000003">
        <property type="entry name" value="heat shock cognate 71 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="625"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="625"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="660" mass="72487" checksum="7EC6D76E95B63D6E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIR9</accession>
    <name>A0A673AIR9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Kin of IRRE-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">kirrel1a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AIR9"/>
    <dbReference type="FunCoup" id="A0A673AIR9">
        <property type="interactions" value="575"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030082.1">
        <property type="protein sequence ID" value="ENSSORP00005029251.1"/>
        <property type="gene ID" value="ENSSORG00005013208.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AIR9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05898">
        <property type="entry name" value="IgI_5_KIRREL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000094">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000103">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF165">
        <property type="entry name" value="KIN OF IRRE LIKE (DROSOPHILA)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025635071" evidence="14">
        <location>
            <begin position="19"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="499"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="19"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="118"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="221"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="306"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="390"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="592"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="694"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="772"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="631"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="792"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="819" mass="88527" checksum="F1924AD700A9E268" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJ35</accession>
    <name>A0A673AJ35_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5 16">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">mpi</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Isomerase that catalyzes the interconversion of fructose-6-P and mannose-6-P and has a critical role in the supply of D-mannose derivatives required for many eukaryotic glycosylation reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="5 16"/>
    <dbReference type="AlphaFoldDB" id="A0A673AJ35"/>
    <dbReference type="FunCoup" id="A0A673AJ35">
        <property type="interactions" value="1193"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029496.1">
        <property type="protein sequence ID" value="ENSSORP00005028683.1"/>
        <property type="gene ID" value="ENSSORG00005013712.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJ35"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00423"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061611">
        <property type="term" value="P:mannose to fructose-6-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.441.10:FF:000001">
        <property type="entry name" value="Mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000044">
        <property type="entry name" value="Mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000060">
        <property type="entry name" value="Putative mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018050">
        <property type="entry name" value="Pmannose_isomerase-type1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046456">
        <property type="entry name" value="PMI_typeI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046458">
        <property type="entry name" value="PMI_typeI_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF0">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20512">
        <property type="entry name" value="PMI_typeI_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00965">
        <property type="entry name" value="PMI_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00966">
        <property type="entry name" value="PMI_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="20">
        <location>
            <begin position="6"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I helical insertion" evidence="21">
        <location>
            <begin position="168"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I C-terminal" evidence="19">
        <location>
            <begin position="334"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF20512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="420" mass="45538" checksum="B6C45A995A4232A4" modified="2020-06-17" version="1">MVPSLFLLSEVPNYAWGKVGLDSEVAQLVVGGDPLAVIEAGKPYAELWMGAHPKGDAQIKDNRIAQTTLGQWIAHFPACLGSKVKDTFHGQLPFLFKVLSVNTALSIQAHPNRDLAARLHAQFPEHYPDNNHKPEMAIALTRFQGLCGFRPVDEILGFLKAVPEFHALVGNEAAEELRCSVGDPVRTSQALKKCFTRMMNCEKKVFVDQLNMLVKRVTEEGAVGKDTSSSNGDLLLCLHSQYPGDIGCFSIYFLNHLVLDPGQAMFLGANEPHAYLYGDCIECMACSDNTVRAGLTPKYIDVNTLCDMLSYSPAPAASKLFPCVQDASDPCVSLYDPPVPDFTVMRIQVPASVKQYTLAPVDSASILLVIEGDATAASAAAHSDVTLRRGTVLFVSANESVSLLITSQTGLTMFRACCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AK25</accession>
    <name>A0A673AK25_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF domain-specific O-linked N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="2">2.4.1.255</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Extracellular O-linked N-acetylglucosamine transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">eogt</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12251"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:90838"/>
            <dbReference type="EC" id="2.4.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48908"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:12252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:90840"/>
            <dbReference type="EC" id="2.4.1.255"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 61 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673AK25"/>
    <dbReference type="FunCoup" id="A0A673AK25">
        <property type="interactions" value="384"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029833.1">
        <property type="protein sequence ID" value="ENSSORP00005029013.1"/>
        <property type="gene ID" value="ENSSORG00005013750.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AK25"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF148">
        <property type="entry name" value="EGF DOMAIN-SPECIFIC O-LINKED N-ACETYLGLUCOSAMINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025641977" description="EGF domain-specific O-linked N-acetylglucosamine transferase" evidence="13">
        <location>
            <begin position="19"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="14">
        <location>
            <begin position="313"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="527" mass="61584" checksum="72D328829BF52D01" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKT4</accession>
    <name>A0A673AKT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ferroxidase</fullName>
            <ecNumber evidence="4">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">hephl1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030009983.1">
        <property type="nucleotide sequence ID" value="XM_030154123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AKT4"/>
    <dbReference type="Ensembl" id="ENSSORT00005031154.1">
        <property type="protein sequence ID" value="ENSSORP00005030305.1"/>
        <property type="gene ID" value="ENSSORG00005012590.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432984"/>
    <dbReference type="CTD" id="101886332"/>
    <dbReference type="InParanoid" id="A0A673AKT4"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000002">
        <property type="entry name" value="Hephaestin like 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF233">
        <property type="entry name" value="FERROXIDASE HEPHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025537443" description="ferroxidase" evidence="16">
        <location>
            <begin position="23"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="18">
        <location>
            <begin position="100"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="18">
        <location>
            <begin position="465"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="17">
        <location>
            <begin position="966"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1099" mass="123441" checksum="BD35DDA8C7917ED0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMV1</accession>
    <name>A0A673AMV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA aspartic acid methyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AMV1"/>
    <dbReference type="Ensembl" id="ENSSORT00005031557.1">
        <property type="protein sequence ID" value="ENSSORP00005030696.1"/>
        <property type="gene ID" value="ENSSORG00005014635.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AMV1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050750">
        <property type="entry name" value="C5-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46098">
        <property type="entry name" value="TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46098:SF1">
        <property type="entry name" value="TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15818" checksum="3CF9DABA1F397514" modified="2020-06-17" version="1">MENLRVLELYSGIGGMHFALKESGIPAHVVAAVDINTTANQVYKHNFPDTPLWNKTIEGIPLDDFNKLSFDMILMSPPCQPFTRYKHHVTQKTSICGSQQSFTFSCSSLKANVSFSIWEVSLTSSIQSSNVLHHCFHVSAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APE6</accession>
    <name>A0A673APE6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FA complementation group D2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fancd2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Fanconi anemia protein FANCD2 family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A673APE6">
        <property type="interactions" value="1219"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032018.1">
        <property type="protein sequence ID" value="ENSSORP00005031149.1"/>
        <property type="gene ID" value="ENSSORG00005014702.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APE6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11721">
        <property type="entry name" value="FANCD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029448">
        <property type="entry name" value="FANCD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32086">
        <property type="entry name" value="FANCONI ANEMIA GROUP D2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32086:SF0">
        <property type="entry name" value="FANCONI ANEMIA GROUP D2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14631">
        <property type="entry name" value="FancD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="841"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1371"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="865"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="884"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1378"/>
            <end position="1388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1432" mass="160415" checksum="2D79A8C060D80B01" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQ17</accession>
    <name>A0A673AQ17_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AQ17"/>
    <dbReference type="Ensembl" id="ENSSORT00005032643.1">
        <property type="protein sequence ID" value="ENSSORP00005031760.1"/>
        <property type="gene ID" value="ENSSORG00005015107.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQ17"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042754">
        <property type="term" value="P:negative regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031602">
        <property type="entry name" value="CIPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34648">
        <property type="entry name" value="CLOCK-INTERACTING PACEMAKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34648:SF7">
        <property type="entry name" value="SI:CH211-132B12.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15800">
        <property type="entry name" value="CiPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025443030" evidence="2">
        <location>
            <begin position="23"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="457" mass="49357" checksum="ADDE0ADE3A0F3899" modified="2020-06-17" version="1">PPPHLLLFLFVWSLIADQINLGSNLSVVQSNPDQLLHSPLAWGGGWHGLSGAKAPTQLLLIQQPAIPAPSSSTLTPSSSVAPPQNHVRKGSGHGSKNSYLPILNSYPRIAPHPRKEGHGSKSASWVQGVKESGSEGHSQSKRVCTEEEKREAVSTTTHLLKPQQKDPRVHSYSQYKGRSSSFPTSSHRPLPGQASSTTKPHHCQQSSSSDSVSSPSVSSSQTPSPPCSSDSPSSSSPPSSSPPSSAAHSPYRLAPDSTSTRQRRFLNTAEILNQSGLLAITLRTKELLKQNAATEREIAQLRQHTNLLCQAAQARHSEGSDSFDKLVHIMTESGSYPDLHIDQVQTLSSSSNNQQSKNQNQEKKEKDVRTTTINPTPTLVSFKKNIDDGTSPPSPLFAPSPETEEREHTPLDLLSSSLSLSFSTSDSKLGQRQAVTDKDLSELSILPESSTHNNYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQY4</accession>
    <name>A0A673AQY4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Activated leukocyte cell adhesion molecule a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">alcama</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AQY4"/>
    <dbReference type="Ensembl" id="ENSSORT00005032592.1">
        <property type="protein sequence ID" value="ENSSORP00005031709.1"/>
        <property type="gene ID" value="ENSSORG00005015090.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQY4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051116">
        <property type="entry name" value="Surface_Rcpt/Adhesion_Mol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11973:SF2">
        <property type="entry name" value="CD166 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11973">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN MUC18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
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            <begin position="503"/>
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            <begin position="31"/>
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    <feature type="domain" description="Ig-like" evidence="19">
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            <begin position="238"/>
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        <location>
            <begin position="401"/>
            <end position="481"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
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            <begin position="534"/>
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        <location>
            <begin position="536"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00004251"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022859"/>
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            <dbReference type="ARBA" id="ARBA00023319"/>
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            <dbReference type="PROSITE" id="PS50835"/>
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    <sequence length="557" mass="60377" checksum="94D5D502969680E6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AS13</accession>
    <name>A0A673AS13_9TELE</name>
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        <submittedName>
            <fullName evidence="12">Zinc finger protein 395-like</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="444" mass="47376" checksum="2B5F7CC846D6D3C0" modified="2020-06-17" version="1">MAAMGPEDRTGTRPDRMAAPPLGQKACTPTPPSTSHSSSTPDTPREGPGPCWTNLPLQILGPTMHLCTGEAAFSFRSPESVEMDEIMAAMVLTSLSCSPGLQSPPQTDPGLQSPPQKDPGLQSPPQTDPEPAPSSSADMECGGGELSDSGSSGYWSWDHGNVSPSPSPSITEADGSPDEGLQMDLDQGEELNAKKPKSSFRGVYKCLWPSCGKVLTSSVGIKRHIRLLHLGSSDQSLREEDFYYTRICCDAVDSASAPAPAPPPSQSRPQALVQSSAPHLSWSSCGSPSGSGLQIPPTHRSRSNSSSGPGPSRPGPLSQSAPGSFWQIHSEHLYQACSPVQVPVPVPVGSRSPSVHGWSVSTSVSCHSNPPVTKARFRSVSVGEQWLQQNRLTTVNAASPTRNHCSYRKARGEAKKCRKVYGLEQKDQWCTACRWKKACQRFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASW4</accession>
    <name>A0A673ASW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">slc26a6</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SULFATE TRANSPORTER"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sulfate_transp"/>
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    </dbReference>
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    </dbReference>
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    </feature>
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    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="699" mass="76546" checksum="ECBD9AC0682815CB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AT02</accession>
    <name>A0A673AT02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Asparagine synthetase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">asnsd1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029980444.1">
        <property type="nucleotide sequence ID" value="XM_030124584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AT02"/>
    <dbReference type="FunCoup" id="A0A673AT02">
        <property type="interactions" value="1807"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033359.1">
        <property type="protein sequence ID" value="ENSSORP00005032469.1"/>
        <property type="gene ID" value="ENSSORG00005015413.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412210"/>
    <dbReference type="CTD" id="54529"/>
    <dbReference type="InParanoid" id="A0A673AT02"/>
    <dbReference type="OrthoDB" id="10252281at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03766">
        <property type="entry name" value="Gn_AT_II_novel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="6">
        <location>
            <begin position="487"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="604" mass="67628" checksum="3222544B14B9C1AC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATN5</accession>
    <name>A0A673ATN5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myozenin 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">myoz1b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myozenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ATN5"/>
    <dbReference type="Ensembl" id="ENSSORT00005033612.1">
        <property type="protein sequence ID" value="ENSSORP00005032714.1"/>
        <property type="gene ID" value="ENSSORG00005015531.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATN5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051373">
        <property type="term" value="F:FATZ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031433">
        <property type="term" value="F:telethonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008438">
        <property type="entry name" value="MYOZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941">
        <property type="entry name" value="MYOZENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941:SF14">
        <property type="entry name" value="MYOZENIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05556">
        <property type="entry name" value="Calsarcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="244" mass="27812" checksum="47E2F15FE5CF1DB0" modified="2020-06-17" version="1">MPVTTPAPPNKRKKANRIITDLSNVSQNDDESDSEAAELDLGTKIKTPKDTMLEELSLLTNKGSKMFKMRQQRVERFIVTNENMQNLENLLMSPPIPPPKPEMPKDEDEEVNTQEEKERRRKAYIRTYVSPWERAMKGNEELTATMKASMPGPIQIHLDLPQYKSFNRTALPFGGFDKACKSLTFELPEVHLAPEEPEPLPSLQADIRSRPSFNRTPIGWVCSDDCTNIHMDLDIPFDGETEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATW5</accession>
    <name>A0A673ATW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115430957</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ATW5"/>
    <dbReference type="FunCoup" id="A0A673ATW5">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033695.1">
        <property type="protein sequence ID" value="ENSSORP00005032794.1"/>
        <property type="gene ID" value="ENSSORG00005015566.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATW5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="154"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="13">
        <location>
            <begin position="296"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="987" mass="111940" checksum="7A64B0BF8D7D3EB3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATX4</accession>
    <name>A0A673ATX4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">prmt1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48104"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:48105"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673ATX4"/>
    <dbReference type="FunCoup" id="A0A673ATX4">
        <property type="interactions" value="2093"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033945.1">
        <property type="protein sequence ID" value="ENSSORP00005033041.1"/>
        <property type="gene ID" value="ENSSORG00005015624.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATX4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000001">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF54">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="13">
        <location>
            <begin position="85"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="14">
        <location>
            <begin position="187"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="364" mass="41821" checksum="D2F645EB3E21CAE9" modified="2020-06-17" version="1">MVLTVDILILSSIPNHGCNCLYLFEVSQGESSVKPAAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNKHLFKDKVVLDVGSGTGILCMFAAKAGAKKVIGIECSSISDYAVKIVKANKLDDVVTIIKGKVEEVELPVEGVDIIISEWMGYCLFYESMLNTVIYARDKWLKPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKEVAIKEPLVDVVDPKQLVSSACLIKEVDIYTVKAEDLTFTAPFCLQVKRNDYIHALVTYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYLDDYLTVKTGEEIFGTISMKPNVKNNRDLDFTIDIDFKGQLCEMSKTSEYRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2I8</accession>
    <name>A0A673B2I8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pou1f1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029981095.1">
        <property type="nucleotide sequence ID" value="XM_030125235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B2I8"/>
    <dbReference type="Ensembl" id="ENSSORT00005035687.1">
        <property type="protein sequence ID" value="ENSSORP00005034763.1"/>
        <property type="gene ID" value="ENSSORG00005016387.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412634"/>
    <dbReference type="CTD" id="5449"/>
    <dbReference type="InParanoid" id="A0A673B2I8"/>
    <dbReference type="OrthoDB" id="6358449at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000150">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF84">
        <property type="entry name" value="NETRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="14">
        <location>
            <begin position="198"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="286"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="288"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="375" mass="40996" checksum="27BC0E1F984F55C9" modified="2020-06-17" version="1">MACQAFTADSFTPLAGDSGLPILMHHASTSDCLSTTSHAHSMVSAVSSGLSLGQTSKRSHMHLSTSSLSNALGNTPPSLHYPVTPCHYGNQQATYGMMTAQEMLSASISQTRILQTCGVPHPNMVSATNPLQGSLTPCLYKFPDHGLSSGSCALSHGFSSLPSAILSTDETPGGPSAGEMKTDIQRKGIRDQEDIPAMDSPQIRELEMFANDFKIRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKAILEKWLDEAELAGALYNDKVGMNERKRKRRTTISLGAKEALERSFVEKSKPSSQEIARIAKGLHLEKEVVRVWFCNRRQREKRVKTSLNLSSSLAKVSPNYITQMSKTQRPMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2N4</accession>
    <name>A0A673B2N4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B2N4"/>
    <dbReference type="Ensembl" id="ENSSORT00005037034.1">
        <property type="protein sequence ID" value="ENSSORP00005036075.1"/>
        <property type="gene ID" value="ENSSORG00005017029.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2N4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="121" mass="13923" checksum="E20CF8394557161E" modified="2020-06-17" version="1">VQAEVNLHTEVQAEVHLHTEVQAEVYLHTEVQAEVHLHTEVQAEVNLHTEVHAEVHLHTEVHAEVYLHTEVHAEVHLHTEVQAEVHLHTEVQAEAEVHLHTEVHAEVYLHTEVHAEVYLHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4E1</accession>
    <name>A0A673B4E1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nrarpa</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Regulates independently canonical Wnt and Notch signaling by modulating LEF1 and Notch protein turnover. Stabilizes LEF1, a pivotal transcription factor in the Wnt signaling cascade, by blocking its ubiquitination. Involved in angiogenesis; involved in intersegmental vessel patterning during development.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NRARP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B4E1"/>
    <dbReference type="SMR" id="A0A673B4E1"/>
    <dbReference type="FunCoup" id="A0A673B4E1">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038066.1">
        <property type="protein sequence ID" value="ENSSORP00005037096.1"/>
        <property type="gene ID" value="ENSSORG00005017419.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B4E1"/>
    <dbReference type="OrthoDB" id="5314041at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019208">
        <property type="term" value="F:phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002043">
        <property type="term" value="P:blood vessel endothelial cell proliferation involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001569">
        <property type="term" value="P:branching involved in blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022407">
        <property type="term" value="P:regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000085">
        <property type="entry name" value="Notch-regulated ankyrin repeat-containing protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051226">
        <property type="entry name" value="PP1_Regulatory_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24179:SF21">
        <property type="entry name" value="MYOSIN BINDING SUBUNIT, ISOFORM O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24179">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="112" mass="12232" checksum="FF1C1915F935B71B" modified="2020-06-17" version="1">MSQADVSTCSAPQRVFQEAVKKGNTKELHSLLQNMTNCEFNVNSFGPEGQTALHQSVIDGNLELVKLLVKFGADIRLANREGWSALHIAAFGGHQDIVLYLITKAKYSSGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5K3</accession>
    <name>A0A673B5K3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ras-related protein Rab-36</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">rab36</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Golgi apparatus membrane</location>
            <topology evidence="15">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673B5K3"/>
    <dbReference type="FunCoup" id="A0A673B5K3">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036888.1">
        <property type="protein sequence ID" value="ENSSORP00005035932.1"/>
        <property type="gene ID" value="ENSSORG00005016934.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5K3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="RAB36, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="266" mass="30380" checksum="A98E7329979A4334" modified="2020-06-17" version="1">MSFGKWCFSPTAYNPSHTNSSSSQCYMPAASLQLKEDWDHEAKAACKDRAIRHQPWDRQKMSKVVVVGDLNVGKTCLINRFCKDVFERDYKATIGVDFEIERFEISGVPFSLQIWDTAGQEKFKCIASAYYRGAHVIITVFDMADIKSLDHTHQWLEEALRENDPDSCFIFLVGTKSDLLSREERLRTEKDAIKIATEMKAEFWAVSSKTGENVQEFFFRVAALSFEKCILRDMENGLPATIGQGDSINRANLEEETPQGMKRRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6K5</accession>
    <name>A0A673B6K5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC115437052</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="20">Pore-forming subunit of a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Voltage-gated sodium (Nav) channels consist of an ion-conducting alpha subunit which is functional on its own associated with regulatory beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Cell membrane</location>
            <topology evidence="1 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005037063.1">
        <property type="protein sequence ID" value="ENSSORP00005036103.1"/>
        <property type="gene ID" value="ENSSORG00005017046.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B6K5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000006">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000005">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1190:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000003">
        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF292">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 22" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 22" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 22" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 22" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9 22" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="678"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="708"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="788"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="876"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="921"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1102"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1142"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1167"/>
            <end position="1185"/>
        </location>
    </feature>
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        <location>
            <begin position="1231"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1341"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1423"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1453"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1487"/>
            <end position="1506"/>
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    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1554"/>
            <end position="1576"/>
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    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1644"/>
            <end position="1667"/>
        </location>
    </feature>
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        <location>
            <begin position="115"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Voltage-gated Na+ ion channel cytoplasmic" evidence="26">
        <location>
            <begin position="496"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="24">
        <location>
            <begin position="677"/>
            <end position="908"/>
        </location>
    </feature>
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        <location>
            <begin position="915"/>
            <end position="1097"/>
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        <location>
            <begin position="1101"/>
            <end position="1372"/>
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        <location>
            <begin position="1422"/>
            <end position="1677"/>
        </location>
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        <location>
            <begin position="1789"/>
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        <location>
            <begin position="30"/>
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        <location>
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        <location>
            <begin position="462"/>
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        <location>
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            <end position="1026"/>
        </location>
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        <source>
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        <source>
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        <source>
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            <dbReference type="RuleBase" id="RU361132"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF06512"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF11933"/>
        </source>
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            <dbReference type="Pfam" id="PF24609"/>
        </source>
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            <dbReference type="Ensembl" id="ENSSORP00005036103.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1903" mass="216381" checksum="6BB30DA8DB879516" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6L3</accession>
    <name>A0A673B6L3_9TELE</name>
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        <submittedName>
            <fullName evidence="3">TAFA chemokine like family member 5</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAFA family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A673B6L3"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <source>
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    </evidence>
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        </source>
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        </source>
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            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
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    <sequence length="131" mass="14572" checksum="10A19A1039DF65E7" modified="2020-06-17" version="1">MESDSLNSYQFSSLVLRSIQININCQLAAGMCEVVTLDRDSSQPRRTIARQTARCACRKGQIAGTTRARPACVDVRIVRSRQWCEMSPCLDHEGCDLLANQSGWTCTQPGGRVKTTTVRAKPELDPTRTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6U7</accession>
    <name>A0A673B6U7_9TELE</name>
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        <submittedName>
            <fullName evidence="14">Myosin-10-like</fullName>
        </submittedName>
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    <gene>
        <name evidence="14" type="primary">LOC115423566</name>
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    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
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    <dbReference type="Ensembl" id="ENSSORT00005039026.1">
        <property type="protein sequence ID" value="ENSSORP00005038036.1"/>
        <property type="gene ID" value="ENSSORG00005002892.1"/>
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    <dbReference type="InParanoid" id="A0A673B6U7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000008">
        <property type="entry name" value="Myosin heavy chain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000007">
        <property type="entry name" value="Myosin heavy chain, non-muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.270.10:FF:000001">
        <property type="entry name" value="Myosin heavy chain, non-muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000009">
        <property type="entry name" value="myosin-11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.270.10">
        <property type="entry name" value="Myosin, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF24">
        <property type="entry name" value="MYOSIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="17">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 2-oxoglutarate + 3 O2 = L-lysyl(4)-[histone H3] + 3 formaldehyde + 3 succinate + 3 CO2</text>
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            <dbReference type="ChEBI" id="CHEBI:61961"/>
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        </reaction>
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        <cofactor evidence="1">
            <name>Fe(2+)</name>
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        </cofactor>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
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        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_PHD"/>
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    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF136">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(4) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21323">
        <property type="entry name" value="KDM5_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="23">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="22">
        <location>
            <begin position="98"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="21">
        <location>
            <begin position="287"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="24">
        <location>
            <begin position="431"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="21">
        <location>
            <begin position="1151"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="21">
        <location>
            <begin position="1446"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1384"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="738"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1405"/>
            <end position="1426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1506" mass="171035" checksum="50BBE44807D6E0F4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9M4</accession>
    <name>A0A673B9M4_9TELE</name>
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        <recommendedName>
            <fullName evidence="10">Dynein axonemal intermediate chain 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">WD repeat-containing protein 78</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
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        <property type="interactions" value="205"/>
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        <property type="protein sequence ID" value="ENSSORP00005038535.1"/>
        <property type="gene ID" value="ENSSORG00005018003.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B9M4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF12">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
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    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="636" mass="71393" checksum="79CE626458B437E5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEP9</accession>
    <name>A0A673BEP9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Exonuclease 1</fullName>
            <ecNumber evidence="17">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115434211</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">5'-&gt;3' double-stranded DNA exonuclease which may also contain a cryptic 3'-&gt;5' double-stranded DNA exonuclease activity. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for DNA mismatch repair (MMR).</text>
    </comment>
    <comment type="function">
        <text evidence="17">5'-&gt;3' double-stranded DNA exonuclease which may also possess a cryptic 3'-&gt;5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A673BEP9"/>
    <dbReference type="FunCoup" id="A0A673BEP9">
        <property type="interactions" value="783"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039852.1">
        <property type="protein sequence ID" value="ENSSORP00005038853.1"/>
        <property type="gene ID" value="ENSSORG00005018163.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BEP9"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:metal ion binding"/>
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        <location>
            <begin position="1"/>
            <end position="99"/>
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            <begin position="138"/>
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            <begin position="356"/>
            <end position="406"/>
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            <begin position="457"/>
            <end position="543"/>
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        <location>
            <begin position="363"/>
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            <begin position="457"/>
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            <begin position="525"/>
            <end position="540"/>
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        <source>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
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        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038853.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="695" mass="76424" checksum="A3EE485029B94BB6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BHI5</accession>
    <name>A0A673BHI5_9TELE</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="6" type="primary">cd40</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    </dbReference>
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    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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            <end position="209"/>
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            <end position="182"/>
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    </feature>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="318" mass="35693" checksum="43A1BA579FB85345" modified="2020-06-17" version="1">MHLLWINLLLLALTVGAPAQPVCDPTTQYEVKGECCKMCPPGTKMTTLGSCQKPECRECEEGRYTDTYTEQQECEKQPYCDPNKKFQRAVHKSKKERTECQCQLGFHCSSESCITCVPHKVCGPGEGAAIKGDHSRDTTCQRCPDGTFSNETSWNSDCKKWTECKSGDQIQKAGTHMSDIVCEPWRSHVVAWVSVLVVVFILVCGVFIWRYRKGKSGNLKICESGWEEKPKQKKEGGMLLPQPVVQVEDESMSQDLLPSQEESRGTPEENDDESGLEIPSDIAFTDNGNYVAQEDGKKEVLSRQESQTQTFSEYKCDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJD9</accession>
    <name>A0A673BJD9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein S100-B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115438907</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030018657.1">
        <property type="nucleotide sequence ID" value="XM_030162797.1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the lid subcomplex of the 19S proteasome regulatory particle complex (also named PA700 complex). The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BRB6"/>
    <dbReference type="Ensembl" id="ENSSORT00005045452.1">
        <property type="protein sequence ID" value="ENSSORP00005044330.1"/>
        <property type="gene ID" value="ENSSORG00005020360.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRB6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000003">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="247"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="441" mass="49290" checksum="ED5B2CE8E62C31C5" modified="2020-06-17" version="1">NYLYFILIEVGPAERSGAEMAAAAVAEFQRAQSLLGTDREASIDILHSIVKRDVQQTDEEAVRVKEQSILELGGLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIQWAKAEKRTFLRQALEARLVSLYFDTQSYQEALQLGSQLLQELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQAALDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPRAITALKYMLLCKIMLNAPEDVQALISGKLALRYAGRQTDSLKCVALASQNRSLADFEQALTEYKSELRDDPIISTHLTKLYHNLLEQNLIRVIEPFSRVQIAHISSIIKLPKGDVERKLSQMILDKKFHGILDQGAGVLIVFEEPVVDQTYESALETIHNMSKVVDSLYNKAKKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRF2</accession>
    <name>A0A673BRF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115431519</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BRF2"/>
    <dbReference type="FunCoup" id="A0A673BRF2">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044286.1">
        <property type="protein sequence ID" value="ENSSORP00005043192.1"/>
        <property type="gene ID" value="ENSSORG00005019918.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRF2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11496">
        <property type="entry name" value="SLC6sbd-TauT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF237">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="514"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="584" mass="64950" checksum="45F5CB165EC83CBB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSK1</accession>
    <name>A0A673BSK1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">camk2a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673BSK1"/>
    <dbReference type="Ensembl" id="ENSSORT00005045900.1">
        <property type="protein sequence ID" value="ENSSORP00005044770.1"/>
        <property type="gene ID" value="ENSSORG00005020595.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BSK1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000002">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000001">
        <property type="entry name" value="calcium/calmodulin-dependent protein kinase type II subunit gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="13"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="498" mass="56489" checksum="D9A750C7A800B136" modified="2020-06-17" version="1">MATVICTRFTEEYQLYEELGKGAFSVVRRCVKVLSGQEYAAKIINTKKLSARDHQKLDREARICRLLKHPNIVRLHDSISEEAHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKSKGAAVKLADFGLAIEVEGDQQAWFGFAGTPGYLSPEVLRKDPYGKAVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRITAAEALKHPWISHRSTVASCMHRQETVECLKKFNARRKLKGAILTTMLATRNFSGTFSKDKYSDICSKYSVLVINGPKVTAVLKVLNISINIIHSDRTETLRKQDIIKVTEQLIEAISNGDFESYTKMCDPAVTAFEPEALGNLVEGLDFHRFYFENLWSKNSKPVHTTILNPHIHLVGDEAACIAYIRVTQYIDANGTPRTAQSEETRVWHRRDGKWQIVHFHRSGSASTLSKYVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSU4</accession>
    <name>A0A673BSU4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BSU4"/>
    <dbReference type="Ensembl" id="ENSSORT00005044759.1">
        <property type="protein sequence ID" value="ENSSORP00005043657.1"/>
        <property type="gene ID" value="ENSSORG00005020150.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BSU4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000002">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF11">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="220" mass="25213" checksum="95375F3509FB64DA" modified="2020-06-17" version="1">MVNKRQREIANLNETLQNIEGSKYYNYLSILFLSLSLSLCLSLSLCPVGPDYKCDEDTEFSCKTNYRCIPQWARCDGTNDYFRCDNHRCVPIRWKCDGNNDCGDGSDERDCRELFCSLSSLQNLCKSIINCKKPRPCSESEFRCDNAQCIPGKWVCDHDNDCGDNSDERDCGEWELHTSSRPLNIFQFFKKLLQNCQPQSIEIHSRGSLGQGLPSLFKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUE2</accession>
    <name>A0A673BUE2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Potassium voltage-gated channel subfamily A member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">kcna1a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BUE2"/>
    <dbReference type="FunCoup" id="A0A673BUE2">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046565.1">
        <property type="protein sequence ID" value="ENSSORP00005045415.1"/>
        <property type="gene ID" value="ENSSORG00005020868.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUE2"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033270">
        <property type="term" value="C:paranode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:action potential"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
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        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KCHANNEL"/>
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        <property type="entry name" value="KV11CHANNEL"/>
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        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
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        <property type="entry name" value="SHAKERCHANEL"/>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="216"/>
            <end position="237"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="322"/>
            <end position="343"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="19">
        <location>
            <begin position="34"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023303"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="491" mass="55716" checksum="AF913A6F184124F2" modified="2020-06-17" version="1">MTVVAGDNMDETSAVPGHPQDTYPPDHNDHECCERVVINIAGLRFETQLKTLSQFPETLLGNPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRPVNVPLDMFSEEIKFYELGVEAMEKFREDEGFIREEERPLPEKEFQRQIWLLFEHPESSGTARGIAIVSVMVILISIVIFCLETLPQLKEDPMGRFERVGNTTIYYKPNILTDPFFVIETLCIIWFSFELIVRFLACPSKPAFFKNMMNMIDIVAIIPYFITLGTELAEDPESEGVGEQATSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEAEEQGSYFGSIPDAFWWAVVSMTTVGYGDMVPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQAQLLNVSNPNIPSETNSSRRSSSTVSKSEYMEIDGDINNSIDNFREANLRTGNCTIANQNCVNKSKLLTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXC3</accession>
    <name>A0A673BXC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Formylglycine-generating enzyme</fullName>
            <ecNumber evidence="15">1.8.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">C-alpha-formylglycine-generating enzyme 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sulfatase-modifying factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">sumf1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Oxidase that catalyzes the conversion of cysteine to 3-oxoalanine on target proteins, using molecular oxygen and an unidentified reducing agent. 3-oxoalanine modification, which is also named formylglycine (fGly), occurs in the maturation of arylsulfatases and some alkaline phosphatases that use the hydrated form of 3-oxoalanine as a catalytic nucleophile. Known substrates include GALNS, ARSA, STS and ARSE.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[sulfatase] + 2 a thiol + O2 = an organic disulfide + 3-oxo-L-alanyl-[sulfatase] + hydrogen sulfide + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51152"/>
            <dbReference type="Rhea" id="RHEA-COMP:12900"/>
            <dbReference type="Rhea" id="RHEA-COMP:12901"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:35489"/>
            <dbReference type="ChEBI" id="CHEBI:85621"/>
            <dbReference type="EC" id="1.8.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Protein modification; sulfatase oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer, homodimer and heterodimer with SUMF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase-modifying factor family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.7" evidence="15"/>
    <dbReference type="RefSeq" id="XP_029989925.1">
        <property type="nucleotide sequence ID" value="XM_030134065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BXC3"/>
    <dbReference type="Ensembl" id="ENSSORT00005047584.1">
        <property type="protein sequence ID" value="ENSSORP00005046425.1"/>
        <property type="gene ID" value="ENSSORG00005021142.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419351"/>
    <dbReference type="CTD" id="285362"/>
    <dbReference type="InParanoid" id="A0A673BXC3"/>
    <dbReference type="OrthoDB" id="659at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120147">
        <property type="term" value="F:formylglycine-generating oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1580.10:FF:000001">
        <property type="entry name" value="Sulfatase modifying factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="entry name" value="paralog of FGE (formylglycine-generating enzyme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051043">
        <property type="entry name" value="Sulfatase_Mod_Factor_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150:SF19">
        <property type="entry name" value="FORMYLGLYCINE-GENERATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23150">
        <property type="entry name" value="SULFATASE MODIFYING FACTOR 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025669720" description="Formylglycine-generating enzyme" evidence="20">
        <location>
            <begin position="20"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase-modifying factor enzyme-like" evidence="21">
        <location>
            <begin position="87"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="368" mass="41083" checksum="75A8F6930F299F39" modified="2020-06-17" version="1">MLRNFALFFVFTCSNTLLSFQTSCGAESGNAAPQTAAGCGCENLKRAAAVEPVEESTATVHPAVKYSKEVHDRPPEAQGDERRVQSQMVLITGGEFLMGTDNPGIPADGEGPQRLVHVDSFYMDIQEVTNQQFEAFVNATGYITEAEKFGDSFVFEGILSETIKNQITQAVAAAPWWLPVKGANWRHPEGPDSNIIDKLDHPVLHVSWTDAVTYCSWANKRLPTEAEWEFACRGGLRDRLYPWGNKLNPKGQHYANLWQGDFPTYNSEEDGYIKTSPVMSFPANGFGLYDMVGNAWEWTSDWWTVHHTTDHKHNPKGPPSGTDKVKKGGSYMCHKSYCYRYRCAARSQNTPDSSSSNVGFRCVSQEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1I2</accession>
    <name>A0A673C1I2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Radical S-adenosyl methionine and flavodoxin domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">tRNA wybutosine-synthesizing protein 1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">tRNA-yW-synthesizing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">tyw1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673C1I2"/>
    <dbReference type="FunCoup" id="A0A673C1I2">
        <property type="interactions" value="681"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047387.1">
        <property type="protein sequence ID" value="ENSSORP00005046228.1"/>
        <property type="gene ID" value="ENSSORG00005021125.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1I2"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="20">
        <location>
            <begin position="87"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="21">
        <location>
            <begin position="397"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="263"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="729" mass="82624" checksum="94108369EF7CFD88" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C211</accession>
    <name>A0A673C211_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">slc31a2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004315.1">
        <property type="nucleotide sequence ID" value="XM_030148455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C211"/>
    <dbReference type="FunCoup" id="A0A673C211">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048153.1">
        <property type="protein sequence ID" value="ENSSORP00005046982.1"/>
        <property type="gene ID" value="ENSSORG00005021504.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429190"/>
    <dbReference type="CTD" id="1318"/>
    <dbReference type="InParanoid" id="A0A673C211"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF8">
        <property type="entry name" value="PROTEIN SLC31A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="157" mass="17264" checksum="A56A3241F3015844" modified="2020-06-17" version="1">MMSMTFEASSSVTLLFDFWDVHGPAGMVLSVFVVLLLTVFYEVLKVWRVWLSQRVTLAPPRSPFAAPPPPCHSTSTATLDNSTSESSLTPIEPIIPTVSTGHSWVLHGVQTLLHMLQVTLGYMLMLCVMSYNLWIFLGVVGGSVLGYFISFPLLGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2Z2</accession>
    <name>A0A673C2Z2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 11">Adenylosuccinate synthetase</fullName>
            <shortName evidence="9">AMPSase</shortName>
            <shortName evidence="9">AdSS</shortName>
            <ecNumber evidence="9 11">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">adss1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9 11">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="9 11"/>
    <dbReference type="AlphaFoldDB" id="A0A673C2Z2"/>
    <dbReference type="FunCoup" id="A0A673C2Z2">
        <property type="interactions" value="942"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050899.1">
        <property type="protein sequence ID" value="ENSSORP00005049696.1"/>
        <property type="gene ID" value="ENSSORG00005022552.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C2Z2"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF2">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE ISOZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="33"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="336"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="368"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="450"/>
            <end position="452"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="462" mass="50725" checksum="CB624D57FBBD1341" modified="2020-06-17" version="1">SSGTDENFAGTGQKRQRNDAGNKATVVLGAQWGDEGKGKVVDLLATEADVVCRCQGGNNAGHTVVVDGKEYDFHLLPSGIINPKSISLIGNGVVIHLPGLFEEGDKNDKKGLKGWEKRLIVSDRAHIGRVCSVELLSCFSTVFDFHQVVDGLQETERQAQEGKNIGTTKKGIGPAYSSKASRTGLRVCDLLGDFKDFSTRFKNLVHQYQSMYPSLTVDVEDQLKKLKDYAERLRPMVRDGVYYMYEALHGPPKKILVEGANAALLDIDFGTYPFVTSSNCTVGGACTGLGIPPLNIGDVFGVAKAYTTRVGIGAFPTEQLNAVGELLQTRGHEVGVTTGRKRRCGWLDLVIVRYAHMINGFTAIALTKLDILDVLDEIKVGVAYKLNGKRIPHFPANMDVLQKVEVEYETFPGWKTDTSAARKWNDLPAKAQNYIRFIENHIGVPIKWVGVGKSRECMIQMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6W6</accession>
    <name>A0A673C6W6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tubulin polymerization-promoting protein family member 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tppp2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPPP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C6W6"/>
    <dbReference type="Ensembl" id="ENSSORT00005052470.1">
        <property type="protein sequence ID" value="ENSSORP00005051246.1"/>
        <property type="gene ID" value="ENSSORG00005023165.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C6W6"/>
    <dbReference type="OrthoDB" id="548799at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032273">
        <property type="term" value="P:positive regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000057">
        <property type="entry name" value="Tubulin polymerization-promoting protein family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008907">
        <property type="entry name" value="TPP/p25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932">
        <property type="entry name" value="P25 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932:SF24">
        <property type="entry name" value="TUBULIN POLYMERIZATION-PROMOTING PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05517">
        <property type="entry name" value="p25-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="178" mass="19156" checksum="2DDCB34C627BA2EA" modified="2020-06-17" version="1">MAEGSVSVAEVETAFQKFAVHGDTKARGKEMNGKNFAKMCKDCQIIDGKNVTTTDVDIVFSKVKAKSARVITFDQFSTALEELALKRFKGKSKEEALQQIYGLIAGKEPANAGVTKVAKAAAVDRLTDTSKYTGSHKERFDETGKGKGKAGRQDVPDTSGYVAAYKGSGTYDDKVKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C726</accession>
    <name>A0A673C726_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Kruppel-like factor 7a</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C726"/>
    <dbReference type="Ensembl" id="ENSSORT00005049819.1">
        <property type="protein sequence ID" value="ENSSORP00005048627.1"/>
        <property type="gene ID" value="ENSSORG00005022152.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C726"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21585">
        <property type="entry name" value="KLF7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000021">
        <property type="entry name" value="Basic krueppel-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001395">
        <property type="entry name" value="Krueppel-like factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF77">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="183"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="213"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="310" mass="35044" checksum="CC60FEAC8DAFAA40" modified="2020-06-17" version="1">MDVLANHSIFQELQLVHDTGYFSAMPSLEENWQQTCLELERYLQTEPKRLSELFDQDLDGLLTPAYLKEDGEEDLSDSLLPSLPILPEPPRVEGVNQAQLSAVTSLTPPSSPELGRHLVKPNQTLTASADGTLTLKLVARKVGLSAARLVAAHALPVRMKDEDEGAACVIGMGELPENKKRIHRCQFNGCRKVYTKSSHLKAHQRTHTGEKPYKCSWEGCEWRFARSDELTRHYRKHTGAKPFKCNHCDRSVKTHLSLLGVKHVARGSELACQRFQFGPWDEFWKCKNYIVKDVEHVLVQGPHTDQCDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7J8</accession>
    <name>A0A673C7J8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C7J8"/>
    <dbReference type="FunCoup" id="A0A673C7J8">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050418.1">
        <property type="protein sequence ID" value="ENSSORP00005049219.1"/>
        <property type="gene ID" value="ENSSORG00005022361.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C7J8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025653078" description="LRRCT domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="37817" checksum="1D972D71606E6E87" modified="2020-06-17" version="1">MNAWFTLAVLCLACFCHGTLSCPALCKCYSRRAEVVCNDVPLTEYPSEGLPRNTTLLTIQFTNITTITERHLNDTPLLQGLHLFSNRLQSLSSHLLRGVSHLNTLDLTDNKLSHLPADVFSHAPLHSLVLKNNLLDKAEADWLSDNSSVTWLDLSGNLLTKIPAGLLQKLPLLENLDLANNRLEKISAKSLDPLTKLERLNLQDNKLNTLDMSIFQNTHNLTNLFLSRNKLQKLPQNLFQELTQLRHLSLDDNQLKHIPPGLLDQLVSLDEEGLDLTANPWMCDGKIEYLWRWLQKNKNKVTCSAPEILKNRQVVSLTDNELSPKKKLDIITNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CA77</accession>
    <name>A0A673CA77_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">psmb1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987097.1">
        <property type="nucleotide sequence ID" value="XM_030131237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CA77"/>
    <dbReference type="FunCoup" id="A0A673CA77">
        <property type="interactions" value="2108"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051413.1">
        <property type="protein sequence ID" value="ENSSORP00005050204.1"/>
        <property type="gene ID" value="ENSSORG00005022777.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417224"/>
    <dbReference type="CTD" id="5689"/>
    <dbReference type="InParanoid" id="A0A673CA77"/>
    <dbReference type="OrthoDB" id="268479at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03757">
        <property type="entry name" value="proteasome_beta_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000033">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="237" mass="26068" checksum="95CDA8D2E1F5E1C1" modified="2020-06-17" version="1">MFSVQTFGDTGKMKDYHYTGPVEHRFSPYAFNGGTVLAVAGEDFAIVASDTRLSEGYSIHSRDSPKCYKLTDTTVIGCSGFHGDCLTLTKIIDARLKMYKHSNNKTMTSGAIAAMLSTILYGRRFFPYYVYNIIGGLDEEGKGAVYSFDPVGSYQRDTYKAGGSASAMLQPLLDNQIGFKNMEGVQHVPLTQDKAVQLVKDVFISAAERDVYTGDALRVCIITKEGIDEQTVALRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAG0</accession>
    <name>A0A673CAG0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxisomal biogenesis factor 11 alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pex11a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029991898.1">
        <property type="nucleotide sequence ID" value="XM_030136038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CAG0"/>
    <dbReference type="FunCoup" id="A0A673CAG0">
        <property type="interactions" value="710"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053804.1">
        <property type="protein sequence ID" value="ENSSORP00005052556.1"/>
        <property type="gene ID" value="ENSSORG00005023675.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420640"/>
    <dbReference type="CTD" id="8800"/>
    <dbReference type="InParanoid" id="A0A673CAG0"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF22">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27661" checksum="644419A3AA4040B5" modified="2020-06-17" version="1">MDAVVKFTNQSQGRDRLLRATQYACSLSSYLLRNKSERKDLVAKLKSLEANMSAGRKLFRLGNTVNSIDAAKRTMQLSDPVLCLCLTVANVNRALYFICDNVLWARNVGLIRDIDKERWSLNASRFYFLSLIMNLTRDAYVISQLMRQKAKDKQFKRKMDAHLNESPEVAEAVVPQLDAFVFLLLESLKSHPAATLDTLKNICDLFIPLDKLGMYQSNAGVVGFCGLLSSLIGIITLAQPKLKIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCZ2</accession>
    <name>A0A673CCZ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">smarcd3b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMARCD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCZ2"/>
    <dbReference type="Ensembl" id="ENSSORT00005053185.1">
        <property type="protein sequence ID" value="ENSSORP00005051945.1"/>
        <property type="gene ID" value="ENSSORG00005023449.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCZ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17676">
        <property type="entry name" value="SWIB_BAF60C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.245.10:FF:000001">
        <property type="entry name" value="SWI/SNF-related matrix-associated regulator of chromatin subfamily D member 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038043">
        <property type="entry name" value="SMARCD3_SWIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="4">
        <location>
            <begin position="255"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="480" mass="55420" checksum="BAA633B5BEF6BE39" modified="2020-06-17" version="1">MATEETAGGARKATKSKLFEFLVHGVRPGMPSGARMPHQGAPMGPPGPPYGGSPAVRPGLPSPVMEPSRKRPAPSQQVQQQQQQQQQQQQQAVQNRRRKMADKILPQRIRELVPESQAYMDLLAFERKLDQTIMRKRVDIQEALKRPMKQKRKLRLYISNTFNPARPDADDSDGSIASWELRVEGKLLDDPGKQKKKFSSFFKSLVIELDKDLYGPDNHLVEWHRTPTTQETDGFQVKRPGDVSVRCTLLLMLDYQPPQFKLDPRLARLLGIHTQTRSCIIQALWQYVKTNKLQDSHDKEYINCDKYFQQIFDCPRLKFSEIPQRLTNLLLPPDPIVINHVISVDPNDQKKTACYDIDVEVEDPLKSQMSSFLLSTANQQEIASLDNKIHETIESINQLKIQRDFMLSFSRDPKGYIQDWLKSQSRDLKLMTDVVGNPEEERRAAFYHEPWSQEAVSRYFYCKIQQRRQELEQALAVRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CD11</accession>
    <name>A0A673CD11_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Potassium voltage-gated channel, subfamily H (eag-related), member 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">kcnh1b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CD11"/>
    <dbReference type="Ensembl" id="ENSSORT00005052423.1">
        <property type="protein sequence ID" value="ENSSORP00005051199.1"/>
        <property type="gene ID" value="ENSSORG00005023133.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CD11"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000003">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000009">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000035">
        <property type="entry name" value="Potassium voltage-gated channel, subfamily H (Eag-related), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003949">
        <property type="entry name" value="K_chnl_volt-dep_EAG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF530">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY H MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01464">
        <property type="entry name" value="EAGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="441"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="20">
        <location>
            <begin position="55"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="544"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="715"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="924"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="891"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="726"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="945" mass="107018" checksum="8C04AEBC2419D6E8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CD41</accession>
    <name>A0A673CD41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">klhl22</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030000194.1">
        <property type="nucleotide sequence ID" value="XM_030144334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CD41"/>
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        <property type="interactions" value="836"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051237.1">
        <property type="protein sequence ID" value="ENSSORP00005050033.1"/>
        <property type="gene ID" value="ENSSORG00005022704.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426304"/>
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    <dbReference type="InParanoid" id="A0A673CD41"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005827">
        <property type="term" value="C:polar microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF5">
        <property type="entry name" value="KELCH-LIKE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="60"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="637" mass="72331" checksum="F53054A79E101B49" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CD45</accession>
    <name>A0A673CD45_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Perilipin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">plin3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CD45"/>
    <dbReference type="Ensembl" id="ENSSORT00005052458.1">
        <property type="protein sequence ID" value="ENSSORP00005051234.1"/>
        <property type="gene ID" value="ENSSORG00005023156.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CD45"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.340">
        <property type="entry name" value="Flagellar protein FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.170">
        <property type="entry name" value="Perilipin, alpha-beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF11">
        <property type="entry name" value="PERILIPIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036881">
        <property type="entry name" value="PAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109775">
        <property type="entry name" value="Mannose-6-phosphate receptor binding protein 1 (Tip47), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="45634" checksum="5E7355C4E18426B4" modified="2020-06-17" version="1">MADSGKTNETEAAAAEHTNGDQQNVVSRVSNLPLVSSACDAVSSAYSSTKDSVPLLKGVMDAAESGVRTLGAAATTGSKPLLDIIEPQLATVNEYAMKGLDKMEETLPILHQPADKVVSDTVGKVYQSVTGAKDAVVGAVMGGVELTRAAVSGGISTVMGTRMGQMVSSGMGLALSRSENWVDQNLPLTERELAALAESTSSDMMSAPSPPASPSYFVRLGKLSAKVQERALEQSLIRARHARDATYAAVAQITSTLDLLESARTSLGTAGNQTGGAPEQLLQQRWTEWKQGQAEAEQTQEEPDGAKDETEQLEWRVLSMVRGLSSQLQSACSNVVSSAQGLPGAVQDQLSSARRSAEELHSSLGNTSALTPLLLERSRQHLTQVQQSLDGVMEYLLNNTPLNWLVGPFAPQLTEKPEANVPKKQDEPHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGA2</accession>
    <name>A0A673CGA2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Golgi reassembly-stacking protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115411739</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GORASP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979819.1">
        <property type="nucleotide sequence ID" value="XM_030123959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CGA2"/>
    <dbReference type="Ensembl" id="ENSSORT00005055882.1">
        <property type="protein sequence ID" value="ENSSORP00005054606.1"/>
        <property type="gene ID" value="ENSSORG00005024445.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411739"/>
    <dbReference type="InParanoid" id="A0A673CGA2"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000026">
        <property type="entry name" value="Golgi reassembly stacking protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000056">
        <property type="entry name" value="Golgi reassembly-stacking protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF1">
        <property type="entry name" value="GOLGI REASSEMBLY-STACKING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="11">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ GRASP-type" evidence="11">
        <location>
            <begin position="112"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="318"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR607583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="398" mass="42768" checksum="E6C9D1E603AB1595" modified="2020-06-17" version="1">MGLPQSAFLLDGGNNSGYHVHGVQQDSPALKAGLEPFFDFILSIGNTRLNKESDLLRDLLKANMEKAVKLEVYNSKTQRMRELEVTPSNMWGGQGLLGASVRFCSFEGASENVWHVLDVEPNSPAALAGLIAHDDYIVGADQVLQDSEDFFSLIEANEGKPLKLLVYNTQTDQCREVVVTPNGAWGGEGCLGCGIGYGYLHRIPTRQVQPNAQSTSIMQSTGASSSEEVVSSAHPEVPSDVTETSLDQIKEAVQDLSVTSPSHQAVDSVADISNILETRTSDLSDAVSTSDIGQSSMFVNYGDDTGDQLSLDNSLFEQRASTPEKEEQPDIQEPELGPGVDLSISAASEVTEDAADPEVTTEILGSRSITEVKDAQSEPSSSDPVMKEEKLDTEVSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CH36</accession>
    <name>A0A673CH36_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zgc:162592</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CH36"/>
    <dbReference type="Ensembl" id="ENSSORT00005052608.1">
        <property type="protein sequence ID" value="ENSSORP00005051383.1"/>
        <property type="gene ID" value="ENSSORG00005023213.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CH36"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF387">
        <property type="entry name" value="HISTAMINE H2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="7"/>
            <end position="25"/>
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        <location>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="297" mass="33499" checksum="D761CB65BC67CC29" modified="2020-06-17" version="1">SRNSRYFLLSLTAADSAFGLLVMPLNLCVSLVKDYTEGPDALCHVVAFCNATVYSTCMYTLASISLERYIAVFYPLKYSSILTRKRTLLLISFSWCFPPFLLLPILFPDGIIEVHFSTASLVCNPSYSTNVGYSLSLTGFIFFPCSIIMSYANLRVWCAAKRQRLKLRKYDLLVPVMAAYYTCWTPCMAVSGSSVPEWAEFVVVWLPTSNGFLNCIFYFWINQSFRRKFRLILQRLILALCPKLANILGCCSSSKAQFISGILENNSVHERSSSVSSTCTLLTLSFSSSQETDIRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CHW1</accession>
    <name>A0A673CHW1_9TELE</name>
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        <submittedName>
            <fullName evidence="11">Uncharacterized LOC115439000</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
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            <end position="245"/>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="245" mass="27258" checksum="D6D40FFE0E0A43D5" modified="2020-06-17" version="1">MNAIIQSFTSNIYSLFLWSISGWTSGSVPESQTVEVQPGENIILSCINKAGQTTVTEWFRVTNRTKVRCISYMFGHDGTASFCDGFGKEKYEMMANPSKIFLHIKDLDFSDSGLYFCGFYLRTHAVISETTYLNVQGNGSDKDANIRTEDEHDGIKILIIAVLGGLSGVLAIVIIALAVKIRKLQTAVNEELQQERNKVNPHTFCNGLSDNLTYAAVNFQAKPKRNRRPASGREMEPNVVYAATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJS8</accession>
    <name>A0A673CJS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphatidylinositol 4-kinase alpha</fullName>
            <ecNumber evidence="4">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pi4kaa</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and HYCC (HYCC1 or HYCC2). Interacts with TMEM150A; regulating recruitment to the plasma membrane. Interacts with TTC7A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="4"/>
    <dbReference type="Ensembl" id="ENSSORT00005057139.1">
        <property type="protein sequence ID" value="ENSSORP00005055851.1"/>
        <property type="gene ID" value="ENSSORG00005024735.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJS8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00871">
        <property type="entry name" value="PI4Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000005">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="19">
        <location>
            <begin position="1505"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="18">
        <location>
            <begin position="1805"/>
            <end position="2083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1441"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1441"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1455"/>
            <end position="1465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2099" mass="236707" checksum="CCC1F83000B03660" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMB9</accession>
    <name>A0A673CMB9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fibronectin type III domain containing 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CMB9"/>
    <dbReference type="Ensembl" id="ENSSORT00005056630.1">
        <property type="protein sequence ID" value="ENSSORP00005055350.1"/>
        <property type="gene ID" value="ENSSORG00005024703.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CMB9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47135">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47135:SF1">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="143"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="314"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="484"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="589" mass="63779" checksum="10FBA1BB8F3351D4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQZ6</accession>
    <name>A0A673CQZ6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CCAAT/enhancer binding protein (C/EBP) 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cebp1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. C/EBP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CQZ6"/>
    <dbReference type="Ensembl" id="ENSSORT00005055682.1">
        <property type="protein sequence ID" value="ENSSORP00005054408.1"/>
        <property type="gene ID" value="ENSSORG00005024371.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CQZ6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030099">
        <property type="term" value="P:myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14693">
        <property type="entry name" value="bZIP_CEBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031106">
        <property type="entry name" value="C/EBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334">
        <property type="entry name" value="CCAAT/ENHANCER BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334:SF62">
        <property type="entry name" value="CCAAT_ENHANCER BINDING PROTEIN (C_EBP) 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="121"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="139"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="199" mass="21802" checksum="AF9EADBD827D744B" modified="2020-06-17" version="1">MSDSRVSSVIQEWASSYPVPAQTLAPPTPAPSGSTSQLAQMDLLSYSQSAALLRGGGGGEDRVSEQMMGLSYLPYAASCLSSGPGGPQTLTRPCVFFCFQDFFLSPTFRAPATKRSITKDSAEYRLRRERNNIAVRKSRDKARRRILLTQQRALQLQEENQKLQIRIGQLTQELDTLKHILSQRPLQGPVEGGSAGSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CR73</accession>
    <name>A0A673CR73_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">HECT domain and RCC1-like domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">HECT-type E3 ubiquitin transferase HERC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">herc2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="Ensembl" id="ENSSORT00005057152.1">
        <property type="protein sequence ID" value="ENSSORP00005055864.1"/>
        <property type="gene ID" value="ENSSORG00005024834.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CR73"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
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    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="4772" mass="521985" checksum="863025CE2356620F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSP2</accession>
    <name>A0A673CSP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sulfotransferase</fullName>
            <ecNumber evidence="20">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">chst2b</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-O-{N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67860"/>
            <dbReference type="Rhea" id="RHEA-COMP:17365"/>
            <dbReference type="Rhea" id="RHEA-COMP:17366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:176490"/>
            <dbReference type="ChEBI" id="CHEBI:176491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3-O-{N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67856"/>
            <dbReference type="Rhea" id="RHEA-COMP:17368"/>
            <dbReference type="Rhea" id="RHEA-COMP:17369"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:176489"/>
            <dbReference type="ChEBI" id="CHEBI:176492"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:67857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-O-{beta-D-galactosyl-(1-&gt;3)-[N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{beta-D-galactosyl-(1-&gt;3)-[6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67868"/>
            <dbReference type="Rhea" id="RHEA-COMP:14419"/>
            <dbReference type="Rhea" id="RHEA-COMP:17367"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:139605"/>
            <dbReference type="ChEBI" id="CHEBI:176494"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:67869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-O-{beta-D-galactosyl-(1-&gt;3)-[N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + 3'-phosphoadenylyl sulfate = 3-O-{beta-D-galactosyl-(1-&gt;3)-[6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1-&gt;6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67864"/>
            <dbReference type="Rhea" id="RHEA-COMP:14420"/>
            <dbReference type="Rhea" id="RHEA-COMP:17370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:139607"/>
            <dbReference type="ChEBI" id="CHEBI:176493"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:67865"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="18">Protein modification; carbohydrate sulfation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homodimer; disulfide-linked. Homodimerization is not essential for enzyme activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network membrane</location>
            <topology evidence="12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A673CSP2"/>
    <dbReference type="FunCoup" id="A0A673CSP2">
        <property type="interactions" value="676"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058671.1">
        <property type="protein sequence ID" value="ENSSORP00005057355.1"/>
        <property type="gene ID" value="ENSSORG00005025443.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSP2"/>
    <dbReference type="OrthoDB" id="6138663at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001517">
        <property type="term" value="F:N-acetylglucosamine 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000765">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016469">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051135">
        <property type="entry name" value="Gal/GlcNAc/GalNAc_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704:SF3">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005883">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="7 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="22">
        <location>
            <begin position="264"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="465" mass="53317" checksum="53C83F57920EFDD9" modified="2020-06-17" version="1">MRGKQYHQPLKLTAPWEKDAGFGRKLKTYRNHTKIIAQPGIVMKVLRRKRIVLFMAYFLLLVLTMLNLANYKWTKEPQQCNHQMRSTTYQSRSDIRFLYRPSLAKKRQLIYVLTTWRSGSSFFGELFNQNPEVFFLYEPMWHIWQKLYPGDAVSLQGAARDMLSSLYRCDLSVFQLYNSPGGKNFTSLGLFGATLNKVVCSYPLCSAYRKEVVGMVDDKVCKKCPPQSLRLLEEECLKYNTIVIKGVRILDVNVLAPLMEDPSLDLKVIHLVRDPRAVANSRIKSRHGLIRENLQVVRSRDPKLRRIPFVDPGHKANKKDGSDYHSIGAMEVICDRTSRTLRTALNPPSWLKGKYMAVRYEDLVENPVKILRSIYRFANLTANHDIESFALNMTSGSSSSSKPFIVSSRNATQAASAWRTVLSIQQIKQVEDYCHHAMSVLGYERVRTAGEAKDLSKSLLTHSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CWW3</accession>
    <name>A0A673CWW3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TBC1 domain family member 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Akt substrate of 160 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tbc1d4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CWW3"/>
    <dbReference type="Ensembl" id="ENSSORT00005057780.1">
        <property type="protein sequence ID" value="ENSSORP00005056483.1"/>
        <property type="gene ID" value="ENSSORG00005025078.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CWW3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00934">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01269">
        <property type="entry name" value="PTB_TBC1D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000003">
        <property type="entry name" value="Putative TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000001">
        <property type="entry name" value="TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2750:FF:000002">
        <property type="entry name" value="TBC1 domain family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000076">
        <property type="entry name" value="TBC1 domain family member 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021785">
        <property type="entry name" value="DUF3350"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF14">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11830">
        <property type="entry name" value="DUF3350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="12">
        <location>
            <begin position="810"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="550"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1088"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="577"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1186" mass="134567" checksum="84A506FD46297010" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673D008</accession>
    <name>A0A673D008_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine protease</fullName>
            <ecNumber evidence="12">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">atg4da</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylserine + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phospho-L-serine</text>
            <dbReference type="Rhea" id="RHEA:67576"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="Rhea" id="RHEA-COMP:17326"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172942"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67577"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A673D008"/>
    <dbReference type="FunCoup" id="A0A673D008">
        <property type="interactions" value="422"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059995.1">
        <property type="protein sequence ID" value="ENSSORP00005058667.1"/>
        <property type="gene ID" value="ENSSORG00005025878.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673D008"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF36">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0645">Protease</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="14">
        <location>
            <begin position="104"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="186"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="465"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="499" mass="55783" checksum="994E70CD1C207142" modified="2020-06-17" version="1">MNSVSPSAVQYVGGVMQDELVETRRQQPPERQSSYSHRPPQTADPSRETSGEPDEMDKLKAKLMSAWNNVKYGWTVKSKTFFNKASPVTVLGHSYLLNSEDEVERFRLAFVSCIWLTYRREFPQLEGSTWTTDCGWGCMLRSGQMLLAQGLLVHLMPRDWAWPDAHQLTDVDFEVFRPRSPARAAGVPIPSFGSPRAPNTPEKSLPSDQVTKNSQKRRPECARDRQAEPLHRRLVTWFGDQPPAPFGVHELVEIGKSSGKKAGDWYGPSIVAHILRKAVAKTSVVHNLAVYVAQDCTVYKEDVVHLCDLSLSQTPPDPSNQAWKSVIILVPVRLGGEALNPSYIECVKNILKLECCIGIIGGKPKHSLFFIGFQDEQLLYLDPHYCQPVVDVSQGNFSLESFHCSSPKKMPFNRMDPSCTIGFYAKNKKDFESLCSAVSVALSSSKEKYPIFTFVEGRGQDYGLEGHSSNHSAPAAHILPPGKLGRSNNRRNSDEFVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y2X3</accession>
    <name>A0A672Y2X3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y2X3"/>
    <dbReference type="Ensembl" id="ENSSORT00005000013.1">
        <property type="protein sequence ID" value="ENSSORP00005000013.1"/>
        <property type="gene ID" value="ENSSORG00005000007.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y2X3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000774">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="321" mass="36999" checksum="5159E988DA012F3E" modified="2020-06-17" version="1">VDDCDSSFQQPGAYTMHLQTHVEEPPEPPGTVHRCDQCGERFTGQSSYREHLSGHSGPYRCEQCGKSFSYFSNYKTHMRVHTGEKPYVCDQCPKSFSFLSSYKRHQVSHSGEKPYQCDFCGKSFSQSGHFSVHLRNHTGEKPFQCDKCGKSFSDVSGYKIHLRVHTGEKPYCCNHCGRGFSQLGNLKSHMRIHTGEKPYRCEDCGQSFSISKTYKQHVLTHTGEKPYKCDECGKGFGRLSNYSRHLRIHTGEQPYRCEQCGQCFNHPHSYNRHLRVHTGEKPYWCSKCKRWFTQPHSLKRHRCVETDDEESTGCIEVRVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5X9</accession>
    <name>A0A672Y5X9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rab42b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y5X9"/>
    <dbReference type="Ensembl" id="ENSSORT00005002028.1">
        <property type="protein sequence ID" value="ENSSORP00005001972.1"/>
        <property type="gene ID" value="ENSSORG00005001223.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y5X9"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001583">
        <property type="entry name" value="RAB42, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="248" mass="28226" checksum="A22C36B2697D613F" modified="2020-06-17" version="1">MVVSQYLAHHQFGSCAFCTYIYPQASTMDLTLWQYQFRIIMLGDSTVGKSSLLKRYTEDLFLESINQTVGVDFYVHFLEVEPGVRVKLQFWDTAGQERFRSVTRSYYRNSVGGLLVFDMTNRASFDHIKEWHAEVCERVQPHKVLFVLVSQKSDRDAHGDRVVSREEAEKLAGHLGMPYVEASAKTGQNVREAFELLARRVYQGLLSGEVELQEGWDGVKCTAPQALQQHRASQSQSSTTPKDKKCCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5Y7</accession>
    <name>A0A672Y5Y7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Si:ch1073-459j12.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neurexin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y5Y7"/>
    <dbReference type="Ensembl" id="ENSSORT00005001621.1">
        <property type="protein sequence ID" value="ENSSORP00005001573.1"/>
        <property type="gene ID" value="ENSSORG00005000820.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y5Y7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000007">
        <property type="entry name" value="Carboxypeptidase X (M14 family), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000007">
        <property type="entry name" value="Carboxypeptidase X, M14 family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF48">
        <property type="entry name" value="ADIPOCYTE ENHANCER-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="F5/8 type C" evidence="9">
        <location>
            <begin position="18"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="10">
        <location>
            <begin position="194"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="640" mass="72954" checksum="5E13336F310BCB76" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6E7</accession>
    <name>A0A672Y6E7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoprotein associated with glycosphingolipid-enriched microdomains 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115428534</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y6E7"/>
    <dbReference type="FunCoup" id="A0A672Y6E7">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002219.1">
        <property type="protein sequence ID" value="ENSSORP00005002162.1"/>
        <property type="gene ID" value="ENSSORG00005001311.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y6E7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050868">
        <property type="term" value="P:negative regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032748">
        <property type="entry name" value="PAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16322">
        <property type="entry name" value="PHOSPHOPROTEIN ASSOCIATED WITH GLYCOSPHINGOLIPID-ENRICHED MICRODOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16322:SF0">
        <property type="entry name" value="PHOSPHOPROTEIN ASSOCIATED WITH GLYCOSPHINGOLIPID-ENRICHED MICRODOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15347">
        <property type="entry name" value="PAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="455" mass="47879" checksum="541EFABCD8E6A140" modified="2020-06-17" version="1">MAPVLNGVWWGPEAGVVGSGAAAAGSVASWLGSEQLVLVCTLSTLTALLLVSVLLLLCAGCQGQKKAVNGHPAGDHENLMNGVSERETISQSADSPATDLAISSSHNGPLTSGTILTDTQDTSPQPSEEMLSSQSELRSSKCPQDRELPSIPPNNALIGDRPPPSGDSTYEVVKEIAAASRDVSVEDSLYETVKELKDPPVQLELPNGTAQLPPDEPPHPAPPTLLNGHLSPCTPERGPLCMGVEYASVDLNKKSRHSADVDAKRHSDNPSVPHKPLEEPEEDLPPPVPEKVLDENDNQPVVVNGLVGAGLHNGELPSPLSPAPGFGNHILSDNESPVYSTVNKTHCDNAVNEEDKEHDYSSIAEIKGLVPASSSSDLYATVNDIYAHPDDPQPGEEPSLDSTDPGYETIRIPKTGSSDDEHRAGQGAEGSDGGKPEPDYESVGELGLSQELSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAJ3</accession>
    <name>A0A672YAJ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YAJ3"/>
    <dbReference type="Ensembl" id="ENSSORT00005001361.1">
        <property type="protein sequence ID" value="ENSSORP00005001324.1"/>
        <property type="gene ID" value="ENSSORG00005000777.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAJ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005076">
        <property type="entry name" value="Glyco_trans_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10462:SF51">
        <property type="entry name" value="GLOBOSIDE ALPHA-1,3-N-ACETYLGALACTOSAMINYLTRANSFERASE 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10462">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03414">
        <property type="entry name" value="Glyco_transf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-galactosamine</name>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-galactosamine</name>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>an alpha-L-fucosyl-(1-&gt;2)-beta-D-galactosyl derivative</name>
            <dbReference type="ChEBI" id="CHEBI:140327"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>an alpha-L-fucosyl-(1-&gt;2)-beta-D-galactosyl derivative</name>
            <dbReference type="ChEBI" id="CHEBI:140327"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>an alpha-L-fucosyl-(1-&gt;2)-beta-D-galactosyl derivative</name>
            <dbReference type="ChEBI" id="CHEBI:140327"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR605076-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR605076-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR605076-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36596" checksum="8F8AEF772B861FBC" modified="2020-06-17" version="1">MFNFLHGRKPTVTVQSPHPIFQSLGMASGREMTDMVVGRAAPPQTSVETPWGAPLVWGDTRNSVWRRAKFAQRDIHTGLLALVVGRYAQFVRRFLSSAETYFLPGRTVTYYILTDNPRTLEPPIELGPERQLKVVPIAEFPGWERLYYRRMALLADAIRDPIGSEVEYIFCADIDQEFVAPVGEEILGDLVATLHPELYGMPRNAFPYEVEETSSAYVEDDEGDYYYTSELYGGLVSEMYRLARACSLLILQDQANGVTARGFEESYLNRYLINHRPTCVLSPEYSWWDSALATNVPVQRLLSLGRQCEAFDMQKREEHKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAX0</accession>
    <name>A0A672YAX0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bestrophin homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Forms chloride channels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YAX0"/>
    <dbReference type="Ensembl" id="ENSSORT00005001637.1">
        <property type="protein sequence ID" value="ENSSORP00005001589.1"/>
        <property type="gene ID" value="ENSSORG00005000997.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAX0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000615">
        <property type="entry name" value="Bestrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021134">
        <property type="entry name" value="Bestrophin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736">
        <property type="entry name" value="BESTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736:SF1">
        <property type="entry name" value="BESTROPHIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01062">
        <property type="entry name" value="Bestrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="111" mass="12731" checksum="92BDF5AA86A2EBA6" modified="2020-06-17" version="1">MIYFEKLAIYCNHYASLIPMSFVLGFYVTLVVNRWWSQYTSIPLPDRLMCILSGGLQGSDERGRLLRRTMMRYASLSALLILRSVSTAVFKRFPTMDHVVEAGECRGRGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YB93</accession>
    <name>A0A672YB93_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YB93"/>
    <dbReference type="Ensembl" id="ENSSORT00005001909.1">
        <property type="protein sequence ID" value="ENSSORP00005001854.1"/>
        <property type="gene ID" value="ENSSORG00005001166.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YB93"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="106" mass="12703" checksum="B1D58217DA473159" modified="2020-06-17" version="1">MLTCDWLLGHRHAHIWCFSTWPWPWEQWIESAFWGFLFSIILLVIMFLWRPSANNQRYAFSPLVDEESEEEEKEPMMNESQSNEQGSNGFLKPLEMLEIQCCVFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBC7</accession>
    <name>A0A672YBC7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RAD54 like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rad54l2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005001989.1">
        <property type="protein sequence ID" value="ENSSORP00005001934.1"/>
        <property type="gene ID" value="ENSSORG00005000641.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YBC7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18069">
        <property type="entry name" value="DEXHc_ARIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044574">
        <property type="entry name" value="ARIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044573">
        <property type="entry name" value="ARIP4_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45797:SF1">
        <property type="entry name" value="HELICASE ARIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45797">
        <property type="entry name" value="RAD54-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="246"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="675"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="906"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1069"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1126"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1183"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1268"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1386"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="622"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="906"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="926"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1090"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1268"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1393"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1407"/>
            <end position="1417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1417" mass="155508" checksum="AFFA68459BA21B0C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBL4</accession>
    <name>A0A672YBL4_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YBL4"/>
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        <property type="protein sequence ID" value="ENSSORP00005000815.1"/>
        <property type="gene ID" value="ENSSORG00005000504.1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="PGBD"/>
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        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="3">
        <location>
            <begin position="108"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="586" mass="67125" checksum="4B109BF2EBC7F6AE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YC34</accession>
    <name>A0A672YC34_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cornifelin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115418588</name>
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    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cornifelin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029988958.1">
        <property type="nucleotide sequence ID" value="XM_030133098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YC34"/>
    <dbReference type="Ensembl" id="ENSSORT00005000182.1">
        <property type="protein sequence ID" value="ENSSORP00005000166.1"/>
        <property type="gene ID" value="ENSSORG00005000135.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418588"/>
    <dbReference type="InParanoid" id="A0A672YC34"/>
    <dbReference type="OrthoDB" id="1045822at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="160" mass="17876" checksum="5A2BD1749CAA8DD7" modified="2020-06-17" version="1">MPQIASETEPFTEWTTHLCDCFEDVSTCCYGFWCCPCLACTVSGRFGENYCLPICDILTPSVMAACGIPVFVPPAVYGMRASIRNRYKIKGSLCKDIGVSCFCVWCAWCQMHRELKHRNKAPAVVNMQNQTVINMQPQMMMPVYPNSGGMVNQAVVVNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YC89</accession>
    <name>A0A672YC89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinase</fullName>
            <ecNumber evidence="8">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115410347</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>1D-myo-inositol 1,3,4,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57660"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.140"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 1,4,5-trisphosphate + 2 ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + 2 ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:32359"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.151"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A672YC89"/>
    <dbReference type="FunCoup" id="A0A672YC89">
        <property type="interactions" value="676"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002572.1">
        <property type="protein sequence ID" value="ENSSORP00005002509.1"/>
        <property type="gene ID" value="ENSSORG00005001493.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YC89"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051765">
        <property type="term" value="F:inositol tetrakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF51">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="337"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="413" mass="46638" checksum="09AB0984B518939D" modified="2020-06-17" version="1">MESSLALGRLELTPGSAPVEVSLAPLLPGAPQAKEKSGQWQGGPPQGRPHLHGCVPLSHQVAGHKYGVDKVGILQHPDGTVLKQLQPPPRGPREMQFYSMVYAEDCCDPCLLELQNHLPKYYGTWSSPDSPNDLYLKLEDVTRRFKKPCIMDVKLGQRSYDPYASQEKREQQIRKYPLMEEIGFLVLGMRVYKVCSDTCDSYDQHYGRGLGKDTVKDGLAKFFHNGASLRKDAVLASIWRVQRILRWFESQRQLTFFASSLLFVYEGLPSSSARPLSSPVGTSSLKAGQEEELTEYNNNNNIQVAMAWDYSLDAMYTNHRKGSHHQCAKGRLHDNSSWKRTGESLQQQKAPNANGNKLQAEREEEESQEIREEVEVRMIDFAHVFPSDSHDQGYIYGLKHLLTVLEQILCDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCR0</accession>
    <name>A0A672YCR0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nupr1b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NUPR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YCR0"/>
    <dbReference type="Ensembl" id="ENSSORT00005000587.1">
        <property type="protein sequence ID" value="ENSSORP00005000561.1"/>
        <property type="gene ID" value="ENSSORG00005000390.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCR0"/>
    <dbReference type="OrthoDB" id="10030453at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018792">
        <property type="entry name" value="NUPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149:SF6">
        <property type="entry name" value="NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149">
        <property type="entry name" value="NUCLEAR PROTEIN 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10195">
        <property type="entry name" value="Phospho_p8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="81" mass="9688" checksum="36A9E78C5A152E00" modified="2020-06-17" version="1">MSHVDVKNLKPTSFEEEYYDQYEYYNLTDKYTEGSSRKGRTKKESSENTNRPNPGGHERKIVEKLQNSEKNRKQDQKQDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YE64</accession>
    <name>A0A672YE64_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ptpn12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_030004637.1">
        <property type="nucleotide sequence ID" value="XM_030148777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YE64"/>
    <dbReference type="Ensembl" id="ENSSORT00005001521.1">
        <property type="protein sequence ID" value="ENSSORP00005001476.1"/>
        <property type="gene ID" value="ENSSORG00005000902.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429395"/>
    <dbReference type="CTD" id="5782"/>
    <dbReference type="InParanoid" id="A0A672YE64"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012266">
        <property type="entry name" value="PTN12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047170">
        <property type="entry name" value="PTN12/18/22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45983">
        <property type="entry name" value="TYROSINE PHOSPHATSE N18, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45983:SF3">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000932">
        <property type="entry name" value="Tyr-Ptase_nr12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="208"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="400"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="753"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="486"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="582"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="616"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="680"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="709"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="753"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="773"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="796" mass="87703" checksum="ED47784435CB8135" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YG22</accession>
    <name>A0A672YG22_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lactase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115412090</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A672YG22">
        <property type="interactions" value="839"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003610.1">
        <property type="protein sequence ID" value="ENSSORP00005003509.1"/>
        <property type="gene ID" value="ENSSORG00005002058.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YG22"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000013">
        <property type="entry name" value="lactase-phlorizin hydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF38">
        <property type="entry name" value="LACTASE_PHLORIZIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025608552" evidence="10">
        <location>
            <begin position="19"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1729"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="1125"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="1600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1768" mass="201376" checksum="69E1506374514B0E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGE1</accession>
    <name>A0A672YGE1_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized LOC115422170</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115422170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
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            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="146"/>
            <end position="173"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="307" mass="33553" checksum="83D3D06BD55D1D7D" modified="2020-06-17" version="1">MSAATASIGDVSRTDRNDSSDANNSGQTQAQCTPMPLPALGSQRIIQGNGTNVGTVISLQCPAKHKLVGGNLMCVMMTNSTQWVGETYCKPLSYNEDYGFRVAVLASIVSSAIIFLMSMAFLTCCLLDCIEKDKREKLERESDAWQWEEQAQQQEHSRSRYNHKGRNNNNNNSQEKVLSLWDAHSPLPCDNSQACRCPQQYGYGWIAPPSARAYSPAPPLPGQDYNQPLLPPDPESAPISSQTQYHKPPPSACQTTNPSLIQISAGGPSMVWQYGGAQHGTLSGITDESNLRNINSAKEFSIRIISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGF1</accession>
    <name>A0A672YGF1_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">CARD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115422171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YGF1"/>
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        <property type="protein sequence ID" value="ENSSORP00005003635.1"/>
        <property type="gene ID" value="ENSSORG00005002184.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YGF1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CARD19"/>
    </dbReference>
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        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34765">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34765:SF1">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19372" checksum="D7B1AE9B1EFD68B6" modified="2020-06-17" version="1">MLQLFHNSEFELLFQLQRDTHFLCTDQRMDTELVDRLVLQLNRTYPQILCDKEAHRFRNLRVPTKVRLADLLKDLYRKGEEACHEFYRGLHIHAEEVYSSLPSRVRHKGPIFFLSCFSFVAGIAMLYYCGETLRSTSPFLQCSAARLSKGAKDILLAYTEAGKHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHT8</accession>
    <name>A0A672YHT8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Hsp90 co-chaperone Cdc37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cdc37</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with Hsp90.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDC37 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YHT8"/>
    <dbReference type="Ensembl" id="ENSSORT00005004267.1">
        <property type="protein sequence ID" value="ENSSORP00005004145.1"/>
        <property type="gene ID" value="ENSSORG00005002409.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YHT8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.610:FF:000001">
        <property type="entry name" value="Hsp90 co-chaperone Cdc37-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF3">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="9">
        <location>
            <begin position="133"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="8">
        <location>
            <begin position="298"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="46"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="379" mass="44468" checksum="CF98D83DEFD50B32" modified="2020-06-17" version="1">MTSQIAGLDYSTWDHIYVSDDEDVTCPFVDTPSLYRMRHRSRLEKMADFQLRGEELETNLEKCKRKLEEAQGRLTELERESKDEEEDREREAELKTVQAEVRKLKKDAKKFEEMIQEYQREKKKLPWNVDTISKDGFSKSVLNIKPETKEETQEEKVEKHKVFVEKYAKEIKHFGMLNRWDDSQKYLSDNPHLVCEETANALVVICIDLEIDEKHALMEQVAHQAIVMQFILDLASAVKADPRGCFRQFFSKIKTADKPYLDAFHHELELLKERVRSCARARMENAMKELEEEEREKRLGPGGLDPVEVYSSLPKDIQRSFDEKNIEMLQEAMSRLDPEEGKYHLKRCIDSGLWVPDSGEADDEEEDDEEHGDVEDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YII1</accession>
    <name>A0A672YII1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pdyn</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the opioid neuropeptide precursor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029995900.1">
        <property type="nucleotide sequence ID" value="XM_030140040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YII1"/>
    <dbReference type="Ensembl" id="ENSSORT00005004519.1">
        <property type="protein sequence ID" value="ENSSORP00005004387.1"/>
        <property type="gene ID" value="ENSSORG00005002662.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423255"/>
    <dbReference type="CTD" id="5173"/>
    <dbReference type="InParanoid" id="A0A672YII1"/>
    <dbReference type="OrthoDB" id="8912385at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031628">
        <property type="term" value="F:opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007600">
        <property type="term" value="P:sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006024">
        <property type="entry name" value="Opioid_neupept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438">
        <property type="entry name" value="PROENKEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438:SF4">
        <property type="entry name" value="PROENKEPHALIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025442923" evidence="5">
        <location>
            <begin position="19"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="236" mass="26834" checksum="5C4D31A4CEDE7CE4" modified="2020-06-17" version="1">MEWYVLVLMLSLPPSIHTDCSTQCHKCMERILIPDASFSRSSLSCAAECEGQLESCGATAAGLRAADFSQDEMAAAEEEGSQQGELVKRYGGFIKRIDKNKNKIFTASPWRDNSILKTAAAAPKNTYEELIKTLEERDVDTGDDSPPADEVLRSYVKRYGGFLRKFGPKTKRSSSQEIREEPEELQKRYGGFMRRIRPKLNNLKWDKRYGGFLRRHFKISVRSAEEPDYYSDEPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMT6</accession>
    <name>A0A672YMT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Origin recognition complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">orc4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the origin recognition complex (ORC) that binds origins of replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4. Interacts with POLQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ORC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YMT6"/>
    <dbReference type="FunCoup" id="A0A672YMT6">
        <property type="interactions" value="1171"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006150.1">
        <property type="protein sequence ID" value="ENSSORP00005005900.1"/>
        <property type="gene ID" value="ENSSORG00005003533.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YMT6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000649">
        <property type="entry name" value="Origin recognition complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016527">
        <property type="entry name" value="ORC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032705">
        <property type="entry name" value="ORC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14629">
        <property type="entry name" value="ORC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007858">
        <property type="entry name" value="ORC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="59"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR007858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="414" mass="47328" checksum="9D742739969A654D" modified="2020-06-17" version="1">MSKRKVKDAHLPVGECITQLQKILRERLCNQQLPEKPEGVGAQHKHLLELLRRTAVHGESNSVLIVGPRGAGKTMLLKSVLRELLEEKEVQKNLLQVQLNGLLQTDDRIALKEITRQLHLENVVGDKVFGSFAENLAFLLEALKKGDRSSSRPVLFVLDEFDLFAHHKNQTLLYNLFDVSQSAQAPIAVVGLTCRLDVLELLEKRYIERVQTQLSLPQDFPDEKFAKDWNESVKALCEDKSVEEVLLRHFNCSRDFRSLHMFLMLCLSRVTVAKPVVKAADVLEAGRLCFADAKADMLHGLSILELCLIIAMKHLNDVYEGEPFNLQMVHNEFKKFLQRKSNSMYNFEQPVVLKAFEHLQQLELIRPVDGSSAKTQREYQLMRLMLDNSQIMEALQKYPQCPTDVKQWAMSAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPA4</accession>
    <name>A0A672YPA4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair protein RAD51 homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rad51c</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPA4"/>
    <dbReference type="Ensembl" id="ENSSORT00005006597.1">
        <property type="protein sequence ID" value="ENSSORP00005006342.1"/>
        <property type="gene ID" value="ENSSORG00005003747.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPA4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033065">
        <property type="term" value="C:Rad51C-XRCC3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000707">
        <property type="term" value="P:meiotic DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19492">
        <property type="entry name" value="Rad51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052093">
        <property type="entry name" value="HR_Repair_Mediator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3 RAD51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="51"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="276"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="33639" checksum="CFDB62B151C3FC79" modified="2020-06-17" version="1">MKPLPFDKCSNNLDLQTGLNHQEALEVLQAVTSDGGGVSFTALELLQKEAELKSIVTFSSQLDAALGGGVPVGKTTEVCGVPGIGKTQLCLQLAVDVQIPECFGGVGGQALFIDTEGSFTVQRLVDLAAAAVRHCSLLVEDDEQRDAMTMFTVETILSNLFLVRCRDYVELLAEVHLLPDFLSQHPNIRLLVIDSVAFPFRLLFDELSQRTRLLNGLMTTRVQQGGQSQLVPALGDSWGHAATLRLLLQWAGSQRMATLFKSSCHMDTTVQYQITSDGFRDTDQSEQPQSKRPRIQTDQSASSQRDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPF1</accession>
    <name>A0A672YPF1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zona pellucida sperm-binding protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Zona pellucida glycoprotein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Zona pellucida protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115421031</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Zona pellucida</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPF1"/>
    <dbReference type="Ensembl" id="ENSSORT00005006690.1">
        <property type="protein sequence ID" value="ENSSORP00005006433.1"/>
        <property type="gene ID" value="ENSSORG00005002279.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPF1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060468">
        <property type="term" value="P:prevention of polyspermy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF31">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0278">Fertilization</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025543163" description="Zona pellucida sperm-binding protein 4" evidence="19">
        <location>
            <begin position="26"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="21">
        <location>
            <begin position="80"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="20">
        <location>
            <begin position="123"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="470" mass="52660" checksum="F6938618D5EC57E2" modified="2020-06-17" version="1">MAKCCSASVLTVVLLGCLVGIQIEAQRVHQSNIQGSLQTPGQPNKQVNVHEPVVKKTPQTQQSSSVQKQGWNQNPQTVVNTCDLRDNVRVVCGHPHISAQECEALNCCFDGHRCYYGKAVTLHCTKSGEFIIVVAKDVTVPLLDLETISLLGQGQGCSHIDSNSNFAIYQFPVTSCGTVVMEDSGSGSLIYENKMISSYEVGISRLGAITRDSQFELVVQCRYHPFLTSTSVQALIFDEVRESIHWHVNGTGSVSVELMLANGECTAKGFEEAYTSFYTEYPVTKALREPVFVAVRLLGMHPTVHLTLGRCWATYGSSPHSLPQWDILINGCPYQDDRYLSKLIRIDSNSGLNFPNHHKRFMFQMFSFVNPQTLSHMSEQVMNILTRSCHTKYIFTYLCFPAQGYSCEPSCPGNSYRNSKGYIYCQFILIKHNSEQVVSTVSKCYVLITYHATVWMLILLFLQLYYILSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YR23</accession>
    <name>A0A672YR23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alcohol dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="9">1.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">S-nitroso-CoA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">akr1a1b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids. Acts as an aldehyde-detoxification enzyme. Also acts as an inhibitor of protein S-nitrosylation by mediating degradation of S-nitroso-coenzyme A (S-nitroso-CoA), a cofactor required to S-nitrosylate proteins. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADPH-dependent reduction of S-nitrosoglutathione. Displays no reductase activity towards retinoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>S-nitroso-CoA + NADPH + H(+) = sulfinamide-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:78375"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145546"/>
            <dbReference type="ChEBI" id="CHEBI:145548"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:78376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-nitrosoglutathione + NADPH + H(+) = S-(hydroxysulfenamide)glutathione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63500"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.2" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A672YR23"/>
    <dbReference type="FunCoup" id="A0A672YR23">
        <property type="interactions" value="1468"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007295.1">
        <property type="protein sequence ID" value="ENSSORP00005007033.1"/>
        <property type="gene ID" value="ENSSORG00005002795.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YR23"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046185">
        <property type="term" value="P:aldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19106">
        <property type="entry name" value="AKR_AKR1A1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000006">
        <property type="entry name" value="Aldo-keto reductase family 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044481">
        <property type="entry name" value="AKR1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="18">
        <location>
            <begin position="31"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="17">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="339" mass="38723" checksum="8A60D9A7F81D3CB8" modified="2020-06-17" version="1">VFYWCKSGFCVYGIDMNYFAVLNTGRKMPLLGLGTWKSQPGQVKQAVLWAFQSGYRHIDCAAVYGNEAEIGDALEETLGHDKILRREDVFITSKLWNSRHHPEDVEPTLLKTLRDLKVDYLDLYLIHWPYAFRRGDVNFPRKEDGTILYDDTHYKETWGAMEKLVGKGLVRAIGLSNFNSQQIDDILSVATIKPAVLQVESHPYLSQEDLLGHCRDRGLVLTAYSPLGSPDRAWKQPDEPVVLDEPVIAALAQKYNKSPAQIILRWQTQRGVVTIPKSVTESRIQENIQVFDFTLEAEEMQTISALNKGWRYIVPMIEVDGQRVPRDAGHPLYPFNLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUC0</accession>
    <name>A0A672YUC0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Leukemia inhibitory factor receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lifra</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YUC0"/>
    <dbReference type="Ensembl" id="ENSSORT00005008464.1">
        <property type="protein sequence ID" value="ENSSORP00005008178.1"/>
        <property type="gene ID" value="ENSSORG00005004562.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YUC0"/>
    <dbReference type="OrthoDB" id="6382334at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048497">
        <property type="entry name" value="LIF-R-like_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040817">
        <property type="entry name" value="LIFR_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21177">
        <property type="entry name" value="LIF-R_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17971">
        <property type="entry name" value="LIFR_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25552">
        <property type="entry name" value="LIFR_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025342298" evidence="12">
        <location>
            <begin position="30"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="702"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="408"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="819"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="890"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="827"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="891"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="942"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="959"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="969"/>
            <end position="986"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="986" mass="109208" checksum="46BE7723DDF7AEA9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z209</accession>
    <name>A0A672Z209_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hook1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the hook family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z209"/>
    <dbReference type="FunCoup" id="A0A672Z209">
        <property type="interactions" value="738"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011094.1">
        <property type="protein sequence ID" value="ENSSORP00005010744.1"/>
        <property type="gene ID" value="ENSSORG00005002286.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z209"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010256">
        <property type="term" value="P:endomembrane system organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000024">
        <property type="entry name" value="Hook homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000091">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008636">
        <property type="entry name" value="Hook_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF36">
        <property type="entry name" value="PROTEIN HOOK HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05622">
        <property type="entry name" value="HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="689"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="163"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="315"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="458"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="714" mass="82494" checksum="1503EC5DA065EEE5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2J3</accession>
    <name>A0A672Z2J3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Programmed cell death 10b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115413439</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PDCD10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z2J3"/>
    <dbReference type="Ensembl" id="ENSSORT00005011352.1">
        <property type="protein sequence ID" value="ENSSORP00005010978.1"/>
        <property type="gene ID" value="ENSSORG00005005933.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2J3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090443">
        <property type="term" value="C:FAR/SIN/STRIPAK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903358">
        <property type="term" value="P:regulation of Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.70:FF:000001">
        <property type="entry name" value="Programmed cell death protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009652">
        <property type="entry name" value="PDCD10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048288">
        <property type="entry name" value="PDCD10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13250:SF1">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13250">
        <property type="entry name" value="TF-1 CELL APOPTOSIS RELATED PROTEIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06840">
        <property type="entry name" value="PDC10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20929">
        <property type="entry name" value="PDCD10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 10 dimerisation" evidence="11">
        <location>
            <begin position="14"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="199" mass="23425" checksum="B9DAFCF81D2DB4AF" modified="2020-06-17" version="1">MMTMEEMKNEAETNSMVSMTLYAVMYPVFNELERINLSAAQTLRAAFIKAERENPGLTQDIIMKILEKKNVPNQLHRIPTAHEFMIDRPEQEFQDLNEKARALKHILSKIPDEINDRVRFLQTIKATDTVNNVIKKYQYQNRRALEHQKKEFVKYSKSFSDTLKTYFKDGNLPEGIHYNNFQIRVNTDGFRAFLFPDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z471</accession>
    <name>A0A672Z471_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Histone acetyltransferase KAT5</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">60 kDa Tat-interactive protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Histone acetyltransferase HTATIP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Lysine acetyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Protein 2-hydroxyisobutyryltransferase KAT5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Protein acetyltransferase KAT5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Protein crotonyltransferase KAT5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Protein lactyltransferase KAT5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">kat5</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2E)-butenoyl-CoA + L-lysyl-[protein] = N(6)-(2E)-butenoyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:53909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(S)-lactoyl-CoA + L-lysyl-[protein] = N(6)-[(S)-lactoyl]-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61996"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:19466"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:231527"/>
            <dbReference type="ChEBI" id="CHEBI:231528"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:61997"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-hydroxyisobutanoyl-CoA + L-lysyl-[protein] = N(6)-(2-hydroxyisobutanoyl)-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:131780"/>
            <dbReference type="ChEBI" id="CHEBI:144968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:24181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z471"/>
    <dbReference type="FunCoup" id="A0A672Z471">
        <property type="interactions" value="1751"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012000.1">
        <property type="protein sequence ID" value="ENSSORP00005011615.1"/>
        <property type="gene ID" value="ENSSORG00005006070.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z471"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046972">
        <property type="term" value="F:histone H4K16 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000022">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000013">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF219">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="29">
        <location>
            <begin position="260"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="70"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="222"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="27">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00073520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00076578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00077013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00078544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00078969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="546" mass="62350" checksum="A68D1430ADD829E6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z490</accession>
    <name>A0A672Z490_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Gamma-aminobutyric acid (GABA) B receptor, 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">gabbr1b</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z490"/>
    <dbReference type="Ensembl" id="ENSSORT00005012020.1">
        <property type="protein sequence ID" value="ENSSORP00005011635.1"/>
        <property type="gene ID" value="ENSSORG00005006188.1"/>
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    <dbReference type="InParanoid" id="A0A672Z490"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004965">
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Complement Module, domain 1"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="GPCR3_GABA-B"/>
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        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
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        <property type="entry name" value="GABA-B RECEPTOR"/>
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        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 1"/>
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        <property type="entry name" value="7tm_3"/>
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        <property type="entry name" value="GABAB1RECPTR"/>
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    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="18"/>
            <end position="38"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="576"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="613"/>
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    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="759"/>
            <end position="780"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="800"/>
            <end position="819"/>
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    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="825"/>
            <end position="845"/>
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    <feature type="domain" description="Sushi" evidence="21">
        <location>
            <begin position="85"/>
            <end position="146"/>
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    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="20">
        <location>
            <begin position="583"/>
            <end position="867"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="862"/>
            <end position="883"/>
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    </feature>
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        <location>
            <begin position="862"/>
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    </feature>
    <feature type="disulfide bond" evidence="17">
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    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000256" key="2">
        <source>
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    <evidence type="ECO:0000256" key="4">
        <source>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011635.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="918" mass="103569" checksum="CCA2CE8B137E4AF1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6N3</accession>
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        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Znf_RING_CS"/>
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    </dbReference>
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        <location>
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            <end position="56"/>
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        <location>
            <begin position="84"/>
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        <location>
            <begin position="249"/>
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        </location>
    </feature>
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            <begin position="141"/>
            <end position="175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="51841" checksum="3FB97B8019E214BD" modified="2020-06-17" version="1">SSHGVLRNNGNYHFLCSICLDVFTDPVSTPCGHNFCHNCIAEHWRVNVPHKCPMCQDVFHSRPQLKTNTFICEMVQRFKQEVQQEDRMCRKHDKPLELFCKPDQTCICLLCTVLDHKNHDVVPLKEEPQYERCRQRTAEGVEVFTGLMESVQKRLDRLKDEIQEKQRTTKKQAQDFINGLELEICELENRRSEVDQLSGSEDHLQVLQRLPSIRASPSMKTWTEVWVRPPSFEGMVVRAVSELEDQLREDMKKVFEAELRRVQQFSVEVTLDPDSAHRELVLSEDRKQVRHGDGRKKLLDKPQRFSVYCNVFGEQRFSSGRSYFEVQVKDKTKWTVGVARESIDRKGTIPLSPAHGFWTIWLRNGDQYEALDEADVHLRLTSSPQKVGVFVDYEEGVVSFYDADSADLIYSFIGCCFTDRLRPFFSPCLSDGGRNSAPLVITPIVHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAH0</accession>
    <name>A0A672ZAH0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZAH0"/>
    <dbReference type="FunCoup" id="A0A672ZAH0">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013921.1">
        <property type="protein sequence ID" value="ENSSORP00005013508.1"/>
        <property type="gene ID" value="ENSSORG00005006984.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZAH0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="310" mass="35634" checksum="83731F89AD1235AE" modified="2020-06-17" version="1">MGLNCLVSYFNLTAYFDTGSLKYMYFILVLSLYLSVIAANVLLIVLICVNRSLHEPMYLFLCSLFVNELYGSTGLFPLLLFQILKDIHTVSVSFCFLQIVCVYSYATVECSTLAVMSYDRYLAICYPLHYNTYMMSHKVFLLVALSWLYSFITMIILISLSAPLQRCGHIIHKVYCDNYSIVKLACSDTTVNNIYGLILTVMVILVPFCYIVYTYMRILKVCFSGSKQTRHKAVSTCTPHLASVINFCFGSRFNLDNVPNMLRIFLSTYWLTCQPLFNPLMYGLNMSKIRIIGPVESRCFFSNRVWDTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAU9</accession>
    <name>A0A672ZAU9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">THAP domain containing 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">thap7</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZAU9"/>
    <dbReference type="Ensembl" id="ENSSORT00005014403.1">
        <property type="protein sequence ID" value="ENSSORP00005013984.1"/>
        <property type="gene ID" value="ENSSORG00005007163.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZAU9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026519">
        <property type="entry name" value="THAP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47502">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47502:SF1">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="264" mass="30339" checksum="463330F193AFEE9E" modified="2020-06-17" version="1">MPRHCSAGGCKSRDNRDTRNAGITFHKLPKRAPRRRLWISNSHRASSWDPQTDFVYFCSKHFTPDSFELTGCSGIRRLKEDAFPTVFESKSVNRFMVGVNTFHITVCCFHRKDLCSADCVDTKEDSDSCPMSDVSSCQMVESPAKDTDKPTPEPPHVVSEVENSAPDSPSRYMRRLPPPPGFYLPKEHSYAQLCPLLWRRRYDQAIDCLAKALRQLHAARRRENRLRSTVLRLRDKRLKHTLTTLVQLNDSCAAYCTCTYEHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDC2</accession>
    <name>A0A672ZDC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDC2"/>
    <dbReference type="Ensembl" id="ENSSORT00005014903.1">
        <property type="protein sequence ID" value="ENSSORP00005014478.1"/>
        <property type="gene ID" value="ENSSORG00005007415.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDC2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
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    </dbReference>
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        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025468738" description="NACHT domain-containing protein" evidence="8">
        <location>
            <begin position="17"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="9">
        <location>
            <begin position="183"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="808" mass="91944" checksum="32A88401BC55ACBA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZF97</accession>
    <name>A0A672ZF97_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Atrophin-1-interacting protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">BAI1-associated protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Membrane-associated guanylate kinase inverted 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Membrane-associated guanylate kinase inverted 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Plays a role in coupling actin fibers to cell junctions in endothelial cells, via its interaction with AMOTL2 and CDH5. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Cilium</location>
            <location evidence="4">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Photoreceptor inner segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Synapse</location>
            <location evidence="19">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A672ZF97">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016154.1">
        <property type="protein sequence ID" value="ENSSORP00005015660.1"/>
        <property type="gene ID" value="ENSSORG00005007151.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZF97"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:late endosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031697">
        <property type="term" value="F:beta-1 adrenergic receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:signaling receptor complex adaptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:SMAD binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070699">
        <property type="term" value="F:type II activin receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06730">
        <property type="entry name" value="PDZ0_MAGI-1_3-like"/>
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    </dbReference>
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        <property type="entry name" value="PDZ1_MAGI-1_3-like"/>
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    </dbReference>
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        <property type="entry name" value="PDZ2_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06733">
        <property type="entry name" value="PDZ3_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ4_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000005">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
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        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
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        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000113">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000150">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000001">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000155">
        <property type="entry name" value="membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000002">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000003">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF27">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16663">
        <property type="entry name" value="MAGI_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
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    <sequence length="1458" mass="159828" checksum="1D4FC3F38730A660" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHV6</accession>
    <name>A0A672ZHV6_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">H(+)-transporting two-sector ATPase</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
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        <name evidence="15" type="primary">atp6v1ab</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="9">Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.</text>
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        <reaction evidence="10">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
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        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
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        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="12">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="13">
        <location>
            <begin position="126"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="11">
        <location>
            <begin position="230"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="14">
        <location>
            <begin position="463"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00006"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02874"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="617" mass="68467" checksum="8CA2DC150E47C320" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJY6</accession>
    <name>A0A672ZJY6_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Aldehyde dehydrogenase family 16 member A1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">aldh16a1</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
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        <scope>IDENTIFICATION</scope>
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            <begin position="62"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="1">
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            <begin position="532"/>
            <end position="760"/>
        </location>
    </feature>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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</entry>
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            <fullName evidence="5">TOG domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="455" mass="51377" checksum="A7400A19A93ECF8A" modified="2020-06-17" version="1">MIRCLEEPGARVTMEALSLRHVFAILLPRLGCRYMFLYQYIGMYLDLLCSSSCSVFDSQTDKHKSGFPSPTTQDESSKKRRGILLSSQTCVCSYAFSNLTTSRRSPIMILGLISQELHEQLLDHRNYQNRTNGVEELKQILSDVDMQTVPSDSVEEFISFLPRLLDDSNFKVLYGTLQVLNLLIQKLDTNLDKYFKQIVLVALKALGDTRTVTRNEYMNVFRHLMKTVAPQQILDLVMGNLKHKNSRIREDVLNIIIAAMLTHPRKDFNIPKLCLEVAPYLADSKRKVRHAALELFAVFDYCLDTGKKQPLMKAVDMVELNEDAEGLMAAVQARRARRVLPKLSSEGVVEYALVVPKPGQRCSMQSPGADLDWVMNGGRISSARSNRTEPDCNRLYGYGSLGSLTDDLPLQRRIVSAGKGKNKLPWEMMSLSSTENDQQCNTPNGKSINLNLSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZP74</accession>
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        <submittedName>
            <fullName evidence="9">Proline-rich protein 36-like</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
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            <taxon>Teleostei</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
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        <text evidence="2">Belongs to the RELT family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="RELT-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31481">
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    </dbReference>
    <dbReference type="Pfam" id="PF12606">
        <property type="entry name" value="RELT"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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            <end position="42"/>
        </location>
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        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="726" mass="78076" checksum="18F48AA09F3BDEB6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZSK7</accession>
    <name>A0A672ZSK7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">T cell leukemia homeobox 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tlx1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZSK7"/>
    <dbReference type="FunCoup" id="A0A672ZSK7">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019925.1">
        <property type="protein sequence ID" value="ENSSORP00005019367.1"/>
        <property type="gene ID" value="ENSSORG00005009523.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZSK7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000040">
        <property type="entry name" value="T-cell leukemia homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042247">
        <property type="entry name" value="TLX1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921">
        <property type="entry name" value="IP01054P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921:SF2">
        <property type="entry name" value="T-CELL LEUKEMIA HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="161"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="286" mass="31663" checksum="3A39CF2D2B54E2EB" modified="2020-06-17" version="1">MDHIGILGTHLQGHTHVEPISFGIDQILSNVEQSCMMGARMHEPDYGHPTYGNGTNGSVNGGGFTCGNAVYNGTANTCGVSSLGGAYINMGVNMNGTNMNSAGVIRVPAHRPLSGGNPSGPSVSGTFNNLTALTFPWMESNRRYTKDRFTVSLSPLTVTRRKKPRTSFTRLQICELEKRFHRQKYLASAERAALAKALKMTDAQVKTWFQNRRTKWRRQTAEEREAERQQANRILMQLQQEAFQKTINQPVTPDPLCLQNSSLFALQNLQPWTENTAKISSVSACD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUP9</accession>
    <name>A0A672ZUP9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ETS domain-containing protein Elk-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">elk4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZUP9"/>
    <dbReference type="FunCoup" id="A0A672ZUP9">
        <property type="interactions" value="1714"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021272.1">
        <property type="protein sequence ID" value="ENSSORP00005020694.1"/>
        <property type="gene ID" value="ENSSORG00005010060.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZUP9"/>
    <dbReference type="OrthoDB" id="10067219at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF21">
        <property type="entry name" value="ETS DOMAIN-CONTAINING PROTEIN ELK-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025598988" evidence="5">
        <location>
            <begin position="22"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="6">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="345"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="476" mass="51580" checksum="204055B2006BB0F0" modified="2020-06-17" version="1">MDNSVTLWQFLLQLLLDSSNEQLICWTNEEGEFKLLQAEEVARLWGARKNKPNMNYDKLSRALRYYYDKNIIKKVNGQKFVYRFVSYPDILKGDVATRTEGGDAAAGGVPTLSRRGDGTLQEVESADRNKVGTNTVALGSSSKQSNRNDYIHSGLYTSFTLNSLQNGRQLFKSIKIENPAEKLAEKRVLSAAAQSQEALPQPQQSTALPSVIKFGNTPPKQAPALSPQVAIETTPMSNHMDPLQVPLPRPEDISAHSTLPSQPVYTFEHIRPAEPPFSLPDLASTSPSPSLVPDSSQELVIDSDMESGSSQPADSQASETVDTQAKEKDVDSGTVSADEPVLVDTDSSSMSSGATTQSSGKTRKPPKILQISPPALFVTTSDFSPMNLCSPSLPTASLTPAMLQTPTLLLTPSPLLSNIHFWSTLSPVAPLSPATRRQGAHLFQFPSVLTPQFQIPVHSMDGTNTPGPISPDPQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUW2</accession>
    <name>A0A672ZUW2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Heparan sulfate 2-O-sulfotransferase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-O-sulfotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">HS 2-O-sulfotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Heparan sulfate 2-sulfotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">hs2st1b</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZUW2"/>
    <dbReference type="FunCoup" id="A0A672ZUW2">
        <property type="interactions" value="873"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020764.1">
        <property type="protein sequence ID" value="ENSSORP00005020197.1"/>
        <property type="gene ID" value="ENSSORG00005009844.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZUW2"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004394">
        <property type="term" value="F:heparan sulfate 2-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000534">
        <property type="entry name" value="Heparan sulfate 2-O-sulfotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF13">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="356" mass="41523" checksum="E6DD7492890954C4" modified="2020-06-17" version="1">MGLLRIMMAPKFQLLAVMAFGVAMLFIENQIQKLEESRAKLERTIARHEVAQVEQRHNEDVGRELAPLSEKDDMVIIYNRVPKTASTSFTNIAYDLCAKNGFHVLHINTTKNNPVMSLQDQVRFVRNVTSWREMKPGFYHGHVAYLDFSKFGVKGKPLYINVVRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVSSGGSDCAPEKLWLQIPFFCGHHSECWNVGSRWALEQAKYNLVNEYLLVGVTEELEDFIMILEAAVPRFFKGATDLYRTGKKSHLRKTTEKKPPTKETIAKLQQSNIWKIENEFYEFALEQFQFVRAHAVREKDGELYILAQSFFYEKIYPKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZV31</accession>
    <name>A0A672ZV31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAAX prenyl protease</fullName>
            <ecNumber evidence="9">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">zmpste24</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M48A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZV31"/>
    <dbReference type="Ensembl" id="ENSSORT00005020829.1">
        <property type="protein sequence ID" value="ENSSORP00005020262.1"/>
        <property type="gene ID" value="ENSSORG00005009876.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZV31"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 9" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 8 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="12">
        <location>
            <begin position="36"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="11">
        <location>
            <begin position="222"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="287"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="465" mass="53222" checksum="078BC7D6FC0DF258" modified="2020-06-17" version="1">MLEIILDLPVEKQIFYAVLGFSWTVYLWEAYLAHRQRRIYRSTTHVPQELGKIMDTETFEKSRLYQLDKSNFSFWSGLYSETEGTLILLLGGIPFLWGVAGSVTARFGFSSEYEITQSLVFLTLATLFSAFTGLPWSLYNTFVIEEKHGFNQQVHGLNRCCKKFAVTQCILLPVTSLLLYHHQDWRRLLFIYAWLFTLAVSLVLVTIYADYIAPLFDKFTPLPDGELKAGIEAMASSISFPLTKVYVVEGSKRSSHSNAYFYGFFKNKRIVLFDTLLEDYSPLNKTAEQQAEQPESNEMDGESKAKPKNKKQGCNNPEILAVLGHELGHWKLGHTVKNIIISQMNSFLCFSLFAVLIGRKELFVAFGFNDSQPTLIGLMIIFQFIFSPYNELLSFCLTVLSRRFEFQADAFARGMGKASELYSALIKLNKDNLGFPVADWLFSMWHYSHPPLLERLRALGNIKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2I1</accession>
    <name>A0A673A2I1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc fingers and homeoboxes protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">zhx1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZHX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030013191.1">
        <property type="nucleotide sequence ID" value="XM_030157331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A2I1"/>
    <dbReference type="Ensembl" id="ENSSORT00005023511.1">
        <property type="protein sequence ID" value="ENSSORP00005022843.1"/>
        <property type="gene ID" value="ENSSORG00005011083.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435123"/>
    <dbReference type="CTD" id="11244"/>
    <dbReference type="InParanoid" id="A0A673A2I1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="905" mass="98513" checksum="4902B3BDB1735EB7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5P2</accession>
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        <submittedName>
            <fullName evidence="13">Spermatogenesis associated 5</fullName>
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        <name evidence="13" type="primary">afg2a</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <text>ATP + H2O = ADP + phosphate + H(+)</text>
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        <text evidence="9">Belongs to the AAA ATPase family. AFG2 subfamily.</text>
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        <property type="entry name" value="AAA"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="12">
        <location>
            <begin position="32"/>
            <end position="117"/>
        </location>
    </feature>
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        <location>
            <begin position="356"/>
            <end position="495"/>
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    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
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            <begin position="630"/>
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    </feature>
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            <begin position="213"/>
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    </feature>
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        <location>
            <begin position="187"/>
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            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="864" mass="93490" checksum="B8298873D06A80FF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A624</accession>
    <name>A0A673A624_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc-binding protein A33-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115420510</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029991656.1">
        <property type="nucleotide sequence ID" value="XM_030135796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A624"/>
    <dbReference type="Ensembl" id="ENSSORT00005024819.1">
        <property type="protein sequence ID" value="ENSSORP00005024108.1"/>
        <property type="gene ID" value="ENSSORG00005011626.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420510"/>
    <dbReference type="InParanoid" id="A0A673A624"/>
    <dbReference type="OrthoDB" id="654191at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12893">
        <property type="entry name" value="SPRY_PRY_TRIM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="14"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="270"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="138"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="470" mass="54213" checksum="8C231F26844C5532" modified="2020-06-17" version="1">MAATSPLCEQDLFCPQCSEIYNLPVLLKCGHNICRVCLQKFWEWKGCRECPVCGTVSVPGRPPINLALKLAAEEYQVLKTTKNKDVCVLHNEKLRIFCQNDEEPICLVCKTSKYHKVHECCPVEEAAQQKKGEISAKLEPLKKKLRVLNKTKQEWEQTKTYIQTQANQDEKAIKEEFEKLRQFLQEEENRRLNLLKQEKETKTRVMCDKLKHIEDQINKLSFIIGDIEKSLRENDLTFLHEYKQTKKRIKCNIQEPECIRDILINSAKHLGLLKFGVWKNMAKIINCAPITLDPNTAQSNLRFSEELTCVQYNSKKLLPDNLERCTSRVCVLGAIGFTSGKHSWTVEVGQGKNWYIGAAQESIKRKSTIFLNPSEGFWVISLSNGDSYWAQTSPRIKLVLKQKPQKITVELDYDKGKVVFINTADSTTIYTFKDKFTERIFPYFSPGLYDDEKKSSPLKICPQTVRVDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6S2</accession>
    <name>A0A673A6S2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A6S2"/>
    <dbReference type="Ensembl" id="ENSSORT00005025728.1">
        <property type="protein sequence ID" value="ENSSORP00005024981.1"/>
        <property type="gene ID" value="ENSSORG00005012041.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A6S2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043447">
        <property type="entry name" value="CCDC120/INAVA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16093:SF5">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16093">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 120 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="387" mass="40852" checksum="388F659C4149436A" modified="2020-06-17" version="1">MLHHTWYKHGHIMVVTCPVSSPFRAEHPEVPQTHKPLPLPSSQPPPLPLQPPPPPPPRSQDSSSSSGPSDPEGGIRGGGVNAARRSNSSDGLLDRITTPEEDGQQGALWVNGLPGTRGGMRSGETDGRAARTNSAGGEGGGRGGGAGRGRGGGGGAGGGGGGGGGGGRPGYSEVLLDYVWGKQQKMQQLQRQQSHNSSRQPITSQLYNGYPAQQPSAAPPTYRGHHGDQRRVKVTRTKSCGPFLPVQQGAEGYADTHNPPLSAAIQPDPHPHLLPPRPQKLPPTQDAQLEEATRSLHKALALEGLRDWYLRNTIGVKGQAGGGGALQRRRTTHGVIQQSTYQTEASHHAAPHHKQAALPHSLSSFMGYCVGIFTVDHIGLYVEPEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A722</accession>
    <name>A0A673A722_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cathepsin Z</fullName>
            <ecNumber evidence="3">3.4.18.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115427339</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity. Capable of producing kinin potentiating peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of C-terminal amino acid residues with broad specificity, but lacks action on C-terminal proline. Shows weak endopeptidase activity.</text>
            <dbReference type="EC" id="3.4.18.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.18.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673A722"/>
    <dbReference type="Ensembl" id="ENSSORT00005025120.1">
        <property type="protein sequence ID" value="ENSSORP00005024407.1"/>
        <property type="gene ID" value="ENSSORG00005011733.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A722"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02698">
        <property type="entry name" value="Peptidase_C1A_CathepsinX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000060">
        <property type="entry name" value="Cathepsin Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033157">
        <property type="entry name" value="CTSZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="70"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="315" mass="35384" checksum="06322514076B3F47" modified="2020-06-17" version="1">RRFSTCCLRVFWFDMAKLITVFIFTFLITAFAARRLFNQKQSCFKPAPRKPDFGVKTAAQPHELLSLAELPKSWDWRNVNGINYASTTRNQHIPQFCGSCWAHASTSAMADRINIKRRGAWPSAYLSVQHVIDCADAGTCYGGDHGLVWEYAHKHGIPDETCNNYQAIDQKCNPFIECGTCTGFGMCNYVQNYTLWKVGDYGAINGRENMMAEIYIHGPISCGIMVTDAFEAYTGGLYSEYVELPGINHVVAVTGWGVENGTEYWIVRNSWGEPWGEKGWAKIVTSTYRGGSGSKYNLALEEDCTYGNVIVPENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABC4</accession>
    <name>A0A673ABC4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ABC4"/>
    <dbReference type="Ensembl" id="ENSSORT00005027255.1">
        <property type="protein sequence ID" value="ENSSORP00005026479.1"/>
        <property type="gene ID" value="ENSSORG00005012679.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABC4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="136" mass="15721" checksum="7198790467F7FCE9" modified="2020-06-17" version="1">MSRSRDTTYFMFLNILLLYSGPEQFSKKAGCFLATHKLQHVPVEDIVVREALSVEKVPEELPQVRVVWLVIKSQGATEVQILSFFLSFFLSFFLSFFLSFFLSFSINDGLCHLKSFVRELGTILYKFPRKVISHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABV3</accession>
    <name>A0A673ABV3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">cdc42bpb</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="Ensembl" id="ENSSORT00005027000.1">
        <property type="protein sequence ID" value="ENSSORP00005026228.1"/>
        <property type="gene ID" value="ENSSORG00005009767.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABV3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01243">
        <property type="entry name" value="PH_MRCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000140">
        <property type="entry name" value="CDC42 binding protein kinase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000014">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001055">
        <property type="entry name" value="Serine/threonine-protein kinase MRCK beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031597">
        <property type="entry name" value="KELK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057529">
        <property type="entry name" value="MRCK/ROCK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014930">
        <property type="entry name" value="Myotonic_dystrophy_kinase_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF34">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MRCK BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08826">
        <property type="entry name" value="DMPK_coil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15796">
        <property type="entry name" value="KELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25346">
        <property type="entry name" value="PH_MRCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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            <end position="340"/>
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            <end position="411"/>
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    <sequence length="1686" mass="189780" checksum="42749EFE64B1B646" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="8">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="191"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="238"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="364"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="406"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="448"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="481" mass="53110" checksum="40F6DE2787BBD2A7" modified="2020-06-17" version="1">MAKGAIIDVERLLIQFQDEDGEVLGSPFDVPLDITVDKLQLVCNALLQKEEPVPLAFFVRGEAEVVSSLGSCVKTLGVETEQVLPVVYQPQAVFKVRAVARCTSTLQGHTEAVISTAFSPTGKYLASGSGDTTVRFWDLMTETPHHTARGHTHWVLSIAWSPDGKKLASGCKNSQIFLWDPATGTQIGKTLTGHTKWITWLCWEPLHLNPECRYLVSSSKDGSVRIWDTVLGRCEKILTGHTHSVTCVKWGGDGLLYTSSQDRTVKVWRAKDGVQCRTLHGHAHWVNTLALSTDYVLRTGAFEPATATVNPQDLTGSLEELKEKALQRYHKVRGSAPERLVSGSDDFTLFLWNPAEDKKPLARMTGHSALVNEVLFSPDTRLLASASFDKSIKIWDGRTGKYLMSLRGHVASVYQVAWSADSRLLVSGSSDSTLKVWDVKTGKLNMDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADH8</accession>
    <name>A0A673ADH8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycine N-acyltransferase-like protein</fullName>
            <ecNumber evidence="1">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115429321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="1"/>
    <dbReference type="RefSeq" id="XP_030004514.1">
        <property type="nucleotide sequence ID" value="XM_030148654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ADH8"/>
    <dbReference type="Ensembl" id="ENSSORT00005028070.1">
        <property type="protein sequence ID" value="ENSSORP00005027284.1"/>
        <property type="gene ID" value="ENSSORG00005013028.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429321"/>
    <dbReference type="InParanoid" id="A0A673ADH8"/>
    <dbReference type="OrthoDB" id="61870at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047961">
        <property type="term" value="F:glycine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013653">
        <property type="entry name" value="GCN5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010313">
        <property type="entry name" value="Glycine_N-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015938">
        <property type="entry name" value="Glycine_N-acyltransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298:SF17">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298">
        <property type="entry name" value="L-COA N-ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08445">
        <property type="entry name" value="FR47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06021">
        <property type="entry name" value="Gly_acyl_tr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="149"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="277" mass="31565" checksum="F305D5EC17AF81D6" modified="2020-06-17" version="1">MKILNNHEMQIAEDVLLKHFPRSLKVYGVLTLNRNKPTTVEVLVDTWPDFNVIICRPDPKNILSSKFGRTVSYYSTDEQILRRMMSEENAVDWSSNLDIHAFDMLNAPMLQQLSSDRGISCRSIVVGRLLYLPDSTSLISPTVDSELESRLSSLDVSHADLVSQSWMFGADEQTVDYIKLLLSNFLSCCITDDQGQPVSWLLVYDFGALGFLYTRPEHRRKGYAKVLISTVAQRRLAQGCPVYAYVDENNSASYRLFKNLGFSDDPSYRAGWFEFNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AFW4</accession>
    <name>A0A673AFW4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AFW4"/>
    <dbReference type="Ensembl" id="ENSSORT00005028952.1">
        <property type="protein sequence ID" value="ENSSORP00005028149.1"/>
        <property type="gene ID" value="ENSSORG00005013464.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AFW4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="130" mass="14291" checksum="4D12C9DDF75FF1C0" modified="2020-06-17" version="1">MSVTNVGRLSSAGRGLNVTCSSTQENHMAVTVVKRLSSAGRGLKITCSFIQEKNHMSVSIVKKLSGKNHMAVTNVKRLSSHQIRLSSNRRDLENTDALKTLKDHTPVTSVVRLSKACLRYNLTAVSTIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHF5</accession>
    <name>A0A673AHF5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Calcium/calmodulin-dependent protein kinase Ia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">camk1a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AHF5"/>
    <dbReference type="Ensembl" id="ENSSORT00005028563.1">
        <property type="protein sequence ID" value="ENSSORP00005027767.1"/>
        <property type="gene ID" value="ENSSORG00005013251.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AHF5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14083">
        <property type="entry name" value="STKc_CaMKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000026">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000203">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="21"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="325"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="375" mass="42412" checksum="7E8115CF32B64C99" modified="2020-06-17" version="1">MPLGEDGNGWKKKTSDIKEHYDFKEVLGTGAFSEVVLAEEKRTQRLVAIKCIPKKALEGKENNIENEIAVLHRIKHPNIVSLEDIFESTSHLYLVMQLVSGGELFDRIVEKGFYTERDASQLIHQILDAVKYLHDMGIVHRDLKPENLLYYSMDEDSKIMISDFGLSKIEGAGSVMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVISYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFICHLMEKDPMKRYTCEQALQHPWICGDTALDKNIHESVSAQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSLEGPSQITPTSPCHGHLLPEEEEEDDDDLGNGEEESLFPNLLHNISLHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AM52</accession>
    <name>A0A673AM52_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115410818</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029978498.1">
        <property type="nucleotide sequence ID" value="XM_030122638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AM52"/>
    <dbReference type="Ensembl" id="ENSSORT00005030320.1">
        <property type="protein sequence ID" value="ENSSORP00005029487.1"/>
        <property type="gene ID" value="ENSSORG00005014082.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410818"/>
    <dbReference type="InParanoid" id="A0A673AM52"/>
    <dbReference type="OrthoDB" id="2018427at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF50">
        <property type="entry name" value="MYOSIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin tail" evidence="4">
        <location>
            <begin position="35"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1127"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="41"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1172" mass="135206" checksum="E921CECEC76749C2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AM74</accession>
    <name>A0A673AM74_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Info WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">wfikkn2a</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WFIKKN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AM74"/>
    <dbReference type="Ensembl" id="ENSSORT00005031642.1">
        <property type="protein sequence ID" value="ENSSORP00005030780.1"/>
        <property type="gene ID" value="ENSSORG00005014683.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AM74"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050431">
        <property type="term" value="F:transforming growth factor beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22605">
        <property type="entry name" value="Kunitz_WFIKKN_1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22606">
        <property type="entry name" value="Kunitz_WFIKKN_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03575">
        <property type="entry name" value="NTR_WFIKKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000002">
        <property type="entry name" value="WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000473">
        <property type="entry name" value="WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000014">
        <property type="entry name" value="WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.75.10:FF:000002">
        <property type="entry name" value="WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45938">
        <property type="entry name" value="ACP24A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45938:SF7">
        <property type="entry name" value="WAP, KAZAL, IMMUNOGLOBULIN, KUNITZ AND NTR DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="4" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025607131" evidence="13">
        <location>
            <begin position="17"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="17">
        <location>
            <begin position="26"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="18">
        <location>
            <begin position="113"/>
            <end position="164"/>
        </location>
    </feature>
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        <location>
            <begin position="197"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="15">
        <location>
            <begin position="315"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="15">
        <location>
            <begin position="373"/>
            <end position="423"/>
        </location>
    </feature>
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        <location>
            <begin position="431"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005743"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="555" mass="61303" checksum="E5BDDBE75073797A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMX3</accession>
    <name>A0A673AMX3_9TELE</name>
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        <submittedName>
            <fullName evidence="8">UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose transporter-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115436696</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AMX3"/>
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        <property type="protein sequence ID" value="ENSSORP00005029767.1"/>
        <property type="gene ID" value="ENSSORG00005013758.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Sugar_P_trans_dom"/>
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        <property type="entry name" value="TPT_transporter"/>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="66"/>
            <end position="317"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="39245" checksum="8F83BFFD887C621C" modified="2020-06-17" version="1">MAQVHKRTHDLGDRKLSAEGASSKPKPATSGDNGVVLKLLAAAFYGVSSFLIVVVNKSLWFDSSLLLQMLATVVVLRTGKMLGVISFPDMDLSIPRKMFPLPLLYVGNQISGLFGTQRLNLPMFTVLRRFSILLTMVFEGILLKKTFSTPIKTTVFTMIFGAFIAASSDLAFDLEGYIFIMLNNVLTAASGAYMKQKLDSKELGKYGLLYYNAVIMILPTVAYAYYVGELQKGLDFAGWTDVLFVVQFVLSCVMGFILMYSIMVCTQYNSALTTTIVGCIKNILVTYIGMVFGGDYIFTWTNFLGLNISIAGSLVYSYITFTQEQTSKNSWINVNHSRSNKLEKEGYIYALVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANM5</accession>
    <name>A0A673ANM5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dispanin subfamily A member 2b-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115421600</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993419.1">
        <property type="nucleotide sequence ID" value="XM_030137559.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A673ANM5">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031836.1">
        <property type="protein sequence ID" value="ENSSORP00005030971.1"/>
        <property type="gene ID" value="ENSSORG00005014771.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421600"/>
    <dbReference type="InParanoid" id="A0A673ANM5"/>
    <dbReference type="OrthoDB" id="9906841at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051517">
        <property type="entry name" value="IFITM_antiviral_protein"/>
    </dbReference>
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        <property type="entry name" value="IFITM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13999">
        <property type="entry name" value="INTERFERON INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="119" mass="12830" checksum="BBE759DD579DE0DE" modified="2020-06-17" version="1">MNPSGHYEPFPLQGGYPGQPAGPSVVQHTVVNISEPPKDHIIWSICTLFYGNPCCLGLAALIYSVKARDRKMVGDLYSARGYGTTAFRINTAAVILICLIFFLIIISVSVSVASASNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANP6</accession>
    <name>A0A673ANP6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">5'-nucleotidase</fullName>
            <ecNumber evidence="14">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115410456</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>CMP + H2O = cytidine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="3.1.3.91"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(7)-methyl-GMP + H2O = N(7)-methylguanosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37107"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:20794"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58285"/>
            <dbReference type="EC" id="3.1.3.91"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrimidine 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A673ANP6"/>
    <dbReference type="Ensembl" id="ENSSORT00005031058.1">
        <property type="protein sequence ID" value="ENSSORP00005030213.1"/>
        <property type="gene ID" value="ENSSORG00005014388.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ANP6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.340:FF:000001">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000032">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.340">
        <property type="entry name" value="Pyrimidine 5'-nucleotidase (UMPH-1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006434">
        <property type="entry name" value="Pyrimidine_nucleotidase_eu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01544">
        <property type="entry name" value="HAD-SF-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045:SF15">
        <property type="entry name" value="7-METHYLGUANOSINE PHOSPHATE-SPECIFIC 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05822">
        <property type="entry name" value="UMPH-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01128">
        <property type="entry name" value="C1.4:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="291" mass="33611" checksum="E9400B6053656DD5" modified="2020-06-17" version="1">MSKNETDIIFILVLKRHQFMVKDPYLILLIILFPQVISDFDMTLTRFAHNGMRVPTTHNILDNRLLINEDCTKKELLNTYYPIEIDAGRSTEEKLPLMVEWWTKVHELLIDQRIRKDMLARAVKESSAMLREGYKVFFDHLAEHHIPLLIFSAGVGDVLEEVIRQNHVFHPNINIISNYMDFDHTGVLQAFKGQLIHTFNKRQGALSHAAHLRQVQDRPNVLLLGDSLGDLTMADGVTDTHNILTIGFLNDQVEERKESYINSFDIVLVKDETMDIPNAIVKYVTSSRYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APD2</accession>
    <name>A0A673APD2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Complex I assembly factor ACAD9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">Acyl-CoA dehydrogenase family member 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">acad9</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">As part of the MCIA complex, primarily participates in the assembly of the mitochondrial complex I and therefore plays a role in oxidative phosphorylation. This moonlighting protein also has a dehydrogenase activity toward a broad range of substrates with greater specificity for long-chain unsaturated acyl-CoAs. However, in vivo, it does not seem to play a primary role in fatty acid oxidation. In addition, the function in complex I assembly is independent of the dehydrogenase activity of the protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E,4Z,7Z,10Z,13Z,16Z,19Z)-docosaheptaenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48184"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74298"/>
            <dbReference type="ChEBI" id="CHEBI:77559"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:48185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(9E)-octadecenoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E,9E)-octadecadienoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48192"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:77537"/>
            <dbReference type="ChEBI" id="CHEBI:77552"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:48193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(9Z)-hexadecenoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E,9Z)-hexadecadienoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:77549"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:47305"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(9Z,12Z)-octadecadienoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E,9Z,12Z)-octadecatrienoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:77558"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:48189"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>decanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-decenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:48177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>eicosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-eicosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:47237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>heptadecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = trans-2-heptadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48196"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74307"/>
            <dbReference type="ChEBI" id="CHEBI:77551"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:48197"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>nonanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-nonenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:76291"/>
            <dbReference type="ChEBI" id="CHEBI:76292"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:48209"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>octadecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-octadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:71412"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:47241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>oxidized [electron-transfer flavoprotein] + (9Z)-octadecenoyl-CoA + H(+) = (2E,9Z)-octadecadienoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47300"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:77553"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:47301"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H(+) = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:43449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>pentadecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48204"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74309"/>
            <dbReference type="ChEBI" id="CHEBI:77545"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:48205"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:47317"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>undecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = trans-2-undecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48200"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:77547"/>
            <dbReference type="ChEBI" id="CHEBI:77548"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48201"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 31">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="28">Homodimer. Interacts with NDUFAF1 and ECSIT. Part of the mitochondrial complex I assembly/MCIA complex that comprises at least the core subunits TMEM126B, NDUFAF1, ECSIT and ACAD9 and complement subunits such as COA1 and TMEM186. Interacts with TMEM70 and TMEM242.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 31">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989424.1">
        <property type="nucleotide sequence ID" value="XM_030133564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673APD2"/>
    <dbReference type="Ensembl" id="ENSSORT00005032126.1">
        <property type="protein sequence ID" value="ENSSORP00005031251.1"/>
        <property type="gene ID" value="ENSSORG00005013078.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418987"/>
    <dbReference type="CTD" id="28976"/>
    <dbReference type="InParanoid" id="A0A673APD2"/>
    <dbReference type="OrthoDB" id="2588832at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01161">
        <property type="entry name" value="VLCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000023">
        <property type="entry name" value="Acyl-CoA dehydrogenase family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000008">
        <property type="entry name" value="acyl-CoA dehydrogenase family member 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000006">
        <property type="entry name" value="very long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000001">
        <property type="entry name" value="Very long-chain-specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049448">
        <property type="entry name" value="ACAD9/ACADV-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF9">
        <property type="entry name" value="COMPLEX I ASSEMBLY FACTOR ACAD9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21343">
        <property type="entry name" value="ACAD9-ACADV_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 31" id="KW-0274">FAD</keyword>
    <keyword evidence="5 31" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 31" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="34">
        <location>
            <begin position="88"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="33">
        <location>
            <begin position="200"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="32">
        <location>
            <begin position="312"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="ACAD9/ACADV-like C-terminal" evidence="35">
        <location>
            <begin position="514"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00055983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00064101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00073945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00076025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="Pfam" id="PF21343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="643" mass="70126" checksum="A97BE566428C8CFC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APK7</accession>
    <name>A0A673APK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial peptide methionine sulfoxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">msraa</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673APK7"/>
    <dbReference type="FunCoup" id="A0A673APK7">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032211.1">
        <property type="protein sequence ID" value="ENSSORP00005031336.1"/>
        <property type="gene ID" value="ENSSORG00005014909.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APK7"/>
    <dbReference type="OrthoDB" id="77405at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000001">
        <property type="entry name" value="Peptide methionine sulfoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF23">
        <property type="entry name" value="PEPTIDE-METHIONINE (S)-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="10">
        <location>
            <begin position="67"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="236" mass="26472" checksum="51B7C7963BAFEA23" modified="2020-06-17" version="1">MVSSSSRLRLVWRHFINSRMGDMAFKSQMPTPETALKGRNESMKVSAKHDVNGNKTVPPFPEETEMAMFGMGCFWGAERKFWKQKGVYSTQVGYSGGFTPNPTYKEVCTGGTGHTEVVRVVFYPDKISFANLLKVFWESHNPTQGMRQGNDVGTTYRSAIYTYTQQQLEEALASRDQYQKVLTEEGFGPITTEIAEAKPFYYAEDYHQQYLSKNPDGYCGLGGTGVSCPIGVKQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARH2</accession>
    <name>A0A673ARH2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pulmonary surfactant-associated protein D-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115421350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ARH2"/>
    <dbReference type="Ensembl" id="ENSSORT00005031841.1">
        <property type="protein sequence ID" value="ENSSORP00005030976.1"/>
        <property type="gene ID" value="ENSSORG00005014776.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031842.1">
        <property type="protein sequence ID" value="ENSSORP00005030977.1"/>
        <property type="gene ID" value="ENSSORG00005014776.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10255512at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000125">
        <property type="entry name" value="Mannan-binding lectin H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF15">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627732" evidence="5">
        <location>
            <begin position="20"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="6">
        <location>
            <begin position="118"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="236" mass="25673" checksum="4937F21E407AB316" modified="2020-06-17" version="1">MRTCLLFCLLCLMVPVSFGQIPGPPGPKGDKGDPGFPGIPARPGSYGLPGLPGPKGDPGPSGPPGFPGPWGPKGPPGYPGPIVMCGRDPHGSVSREFEDLKKILENLQRLIKYEFIRRAGQKYFVSFKERGSFSKAVDFCSQQGLQLALPQNEEENNALTQVFGEGDKTAWISINNRKAQGNFAVDLKNQPLVFTKWAEGQPDRSVQDTGCTMLTENGFWTVTQECSLQAYIICQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AT33</accession>
    <name>A0A673AT33_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Synuclein, gamma b (breast cancer-specific protein 1)</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the synuclein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AT33"/>
    <dbReference type="Ensembl" id="ENSSORT00005033639.1">
        <property type="protein sequence ID" value="ENSSORP00005032741.1"/>
        <property type="gene ID" value="ENSSORG00005015544.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033640.1">
        <property type="protein sequence ID" value="ENSSORP00005032742.1"/>
        <property type="gene ID" value="ENSSORG00005015545.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903136">
        <property type="term" value="F:cuprous ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.700">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001058">
        <property type="entry name" value="Synuclein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820:SF10">
        <property type="entry name" value="GAMMA-SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820">
        <property type="entry name" value="SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01387">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01211">
        <property type="entry name" value="SYNUCLEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118375">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="110" mass="11269" checksum="B87B60194F196A2B" modified="2020-06-17" version="1">MDVLMKGFSMAKEGVVAAAEKTKAGMEEAAAKTKEGVMYVGNKTKEGVVSSVNTVANRTVDQTNIVADTAVAGANEVSQAAVEGVENVAASTGMMNQVGYHGNEVNSGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATZ7</accession>
    <name>A0A673ATZ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Arachidonate--CoA ligase</fullName>
            <ecNumber evidence="12">6.2.1.15</ecNumber>
            <ecNumber evidence="13">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">acsl2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52116"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.15" evidence="12"/>
    <dbReference type="EC" id="6.2.1.3" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A673ATZ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005032686.1">
        <property type="protein sequence ID" value="ENSSORP00005031802.1"/>
        <property type="gene ID" value="ENSSORG00005006733.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATZ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF36">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="16">
        <location>
            <begin position="129"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="736" mass="82259" checksum="C5E70F222D0335C1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AW43</accession>
    <name>A0A673AW43_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 168-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tmem168b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM168 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AW43"/>
    <dbReference type="FunCoup" id="A0A673AW43">
        <property type="interactions" value="805"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033435.1">
        <property type="protein sequence ID" value="ENSSORP00005032543.1"/>
        <property type="gene ID" value="ENSSORG00005015443.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AW43"/>
    <dbReference type="OrthoDB" id="5967342at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd21494">
        <property type="entry name" value="TMEM168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029713">
        <property type="entry name" value="TMEM168"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14437">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14437:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="656"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="700" mass="78987" checksum="AD7DFEC58427233D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0C1</accession>
    <name>A0A673B0C1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">agpat3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029985393.1">
        <property type="nucleotide sequence ID" value="XM_030129533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B0C1"/>
    <dbReference type="FunCoup" id="A0A673B0C1">
        <property type="interactions" value="703"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034871.1">
        <property type="protein sequence ID" value="ENSSORP00005033958.1"/>
        <property type="gene ID" value="ENSSORG00005016060.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415875"/>
    <dbReference type="CTD" id="56894"/>
    <dbReference type="InParanoid" id="A0A673B0C1"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF9">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="90"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="377" mass="43738" checksum="73CB9DC419E95A8E" modified="2020-06-17" version="1">MALLAYLKSLFILQLLMGFVFVVSGLIINFIQLCTCILWPINKQLYRRINCRLSYSLWSQLVMLLEWWSGTDCVLYTDQATVDKFGKEHVIIILNHNYEIDFLCGWTMCERFGVLGSSKVLAKHELLKVPLIGWTWYFLEIVFCKRKWEEDRKTVFKGLGRLKDYPEYMWFLLYCEGTRFTEKKHQISMQVAESKGLPKLKYHLLPRTKGFTTTLQCLKGTVTAVYDVTLNFKDNQTPTLLGIVNGKKYKADMSVRRFPVEDIPDDEQECANWLHKLYQEKDALQEIYHKEGKFPGPTIIPRRRLWTLLNFLFWATLLLSPLIKFACGVVVSGSPLLIIGFIIFLIIASIAIRRLIGVTEVKKTGSSYGDQEAKKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B278</accession>
    <name>A0A673B278_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 238-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115415984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029985523.1">
        <property type="nucleotide sequence ID" value="XM_030129663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B278"/>
    <dbReference type="FunCoup" id="A0A673B278">
        <property type="interactions" value="615"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035565.1">
        <property type="protein sequence ID" value="ENSSORP00005034643.1"/>
        <property type="gene ID" value="ENSSORG00005016328.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415984"/>
    <dbReference type="InParanoid" id="A0A673B278"/>
    <dbReference type="OrthoDB" id="8829850at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029365">
        <property type="entry name" value="TMEM238"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613">
        <property type="entry name" value="SI:CH211-232M10.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15125">
        <property type="entry name" value="TMEM238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="102" mass="10749" checksum="409AC857FDECDAB0" modified="2020-06-17" version="1">MGRCAGLSHCKLALAFAVLMDVLGGGAGGGLRPLGDPGQDFGDLLVYTGALLVLMSLGGWVLWYSGNIEGLTSRKDLGHIGSTVDRLARNLSRKILSYRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5X1</accession>
    <name>A0A673B5X1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673B5X1"/>
    <dbReference type="FunCoup" id="A0A673B5X1">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037005.1">
        <property type="protein sequence ID" value="ENSSORP00005036047.1"/>
        <property type="gene ID" value="ENSSORG00005017016.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5X1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF10">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 13 ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="106"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="6">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="195" mass="21978" checksum="F793E58A1CE1AC1D" modified="2020-06-17" version="1">MSLRNPPYEPPSVSELQEFLLTDRKPTGHVNQVWPNLYIGNEVAARDKGVLHSLGITHIVNAAHGPANPGPGPCFYVKTGHRYYREMSVDYYGVEADDAIDFILSPYFYPTARYIRAALNMGGHVFVHCLMGVSRSATLVLAFLMICEGLQLQEAVAAVRPHRDICPNPGFLQQLRSLDMSLERERRRRQQAQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB51</accession>
    <name>A0A673BB51_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRR15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BB51"/>
    <dbReference type="Ensembl" id="ENSSORT00005040058.1">
        <property type="protein sequence ID" value="ENSSORP00005039055.1"/>
        <property type="gene ID" value="ENSSORG00005018255.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BB51"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028237">
        <property type="entry name" value="PRR15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15321">
        <property type="entry name" value="ATAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="90" mass="10547" checksum="228CF773F0F89E54" modified="2020-06-17" version="1">MTERIPWWRAFLPKRKSGGSKDAGSKDTVQERPKEPTPAASKTTADQSTCRRNMRVSRSGRFKERRRQRQSLPIQENDTENLKREDKEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BBE7</accession>
    <name>A0A673BBE7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosylation factor-like protein 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ADP-ribosylation factor 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115431373</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase that recruits MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with ARL14EP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BBE7"/>
    <dbReference type="FunCoup" id="A0A673BBE7">
        <property type="interactions" value="1014"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038924.1">
        <property type="protein sequence ID" value="ENSSORP00005037937.1"/>
        <property type="gene ID" value="ENSSORG00005017790.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BBE7"/>
    <dbReference type="OrthoDB" id="6228084at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="18"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="206" mass="23142" checksum="6C95D3A883E82162" modified="2020-06-17" version="1">MGIHGSKPQKHVQVLILGLDGSGKTTLLYKLKYNESVVTVPTVGFNVETLETERSSPGLTVWDVGGQRKMRPHWKHHYADTAGLVFVVDSSDHKRLDEARKELHRVLRYESLREVPLVVLANKQDLPEALSPEVLCLKLDLRKVCEGRAWFIQPCSAATGMGLEEGFRRIVYLMKTPLKQTQEDIKIKMRSKGLGVTALKQVLLCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BBU7</accession>
    <name>A0A673BBU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ubiquitin-ribosomal protein eS31 fusion protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the 40S subunit of the ribosome. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Part of the 40S ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BBU7"/>
    <dbReference type="Ensembl" id="ENSSORT00005039749.1">
        <property type="protein sequence ID" value="ENSSORP00005038754.1"/>
        <property type="gene ID" value="ENSSORG00005018101.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BBU7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000158">
        <property type="entry name" value="Polyubiquitin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000160">
        <property type="entry name" value="Polyubiquitin-C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000009">
        <property type="entry name" value="Ubiquitin-60S ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="69"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="119"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="197"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="273"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="349"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="443" mass="50123" checksum="574618CA78837707" modified="2020-06-17" version="1">MQIFVKTLTGKTITLEVEPSDTIENVKQGLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKLEDGRTLSDYIQKESTLHWKDITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLFYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPQDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGSKVEMLNFHVFTRFTITNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCT3</accession>
    <name>A0A673BCT3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Centrosomal protein 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">FGFR1OP N-terminal-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">LisH domain-containing protein FOPNL</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cep20</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the biogenesis of cilia. Required for the recruitment of PLK1 to centrosomes and S phase progression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
            <location evidence="3">Centriolar satellite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP43 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BCT3"/>
    <dbReference type="FunCoup" id="A0A673BCT3">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039230.1">
        <property type="protein sequence ID" value="ENSSORP00005038238.1"/>
        <property type="gene ID" value="ENSSORG00005017894.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BCT3"/>
    <dbReference type="OrthoDB" id="5970631at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.40:FF:000002">
        <property type="entry name" value="LisH domain-containing protein FOPNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018993">
        <property type="entry name" value="FOP_dimerisation-dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF19">
        <property type="entry name" value="CENTROSOMAL PROTEIN 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09398">
        <property type="entry name" value="FOP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGFR1 oncogene partner (FOP) N-terminal dimerisation" evidence="13">
        <location>
            <begin position="43"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="146" mass="15908" checksum="D410563F6874BF7C" modified="2020-06-17" version="1">MASVTEMKCAVRETLESRGVLGQLKARIRAEVFRALDDEHRPRPPLSHENLLINELIREYLEFNKYRHTASVLTAESGQPEVPLDRQFLANELKVAEDASSKSVPLLYGLVSHFVNSGDNATKVFLRGASLPSGPAAGSAVPGPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCU4</accession>
    <name>A0A673BCU4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">High mobility group protein B1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115432682</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HMGB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030009452.1">
        <property type="nucleotide sequence ID" value="XM_030153592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BCU4"/>
    <dbReference type="FunCoup" id="A0A673BCU4">
        <property type="interactions" value="1652"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039429.1">
        <property type="protein sequence ID" value="ENSSORP00005038437.1"/>
        <property type="gene ID" value="ENSSORG00005017958.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432682"/>
    <dbReference type="CTD" id="321622"/>
    <dbReference type="InParanoid" id="A0A673BCU4"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21978">
        <property type="entry name" value="HMG-box_HMGB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21979">
        <property type="entry name" value="HMG-box_HMGB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000006">
        <property type="entry name" value="High mobility group protein B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000015">
        <property type="entry name" value="high mobility group protein B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017967">
        <property type="entry name" value="HMG_boxA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF12">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN B1-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00353">
        <property type="entry name" value="HMG_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HMG box" evidence="10">
        <location>
            <begin position="8"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="10">
        <location>
            <begin position="94"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="8"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="94"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="162"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="205" mass="23395" checksum="5E8D275F0ED0343B" modified="2020-06-17" version="1">MGKEVGKPRGKMSSYAYFVQTCREEHKKKHPDASVNFAEFSKKCSERWKTMSSKEKSKFEDLAKQDKVRYEREMLNYVPARGAKKKKYKDPNAPKRPPSAFFIFCSEFRPKVKGENPGLTIGDVAKKLGEMWNSTAAEDKQPYEKKASKLKEKYEKDIAAYRAKGKAGGGAPAKAPAKVEKKDDDDDEDEDDEEEDEDDDEDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEB3</accession>
    <name>A0A673BEB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lysyl oxidase homolog</fullName>
            <ecNumber evidence="12">1.4.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">loxl5a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-lysyl-[protein] + O2 + H2O = (S)-2-amino-6-oxohexanoyl-[protein] + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24544"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:12448"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:131803"/>
            <dbReference type="EC" id="1.4.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>lysine tyrosylquinone residue</name>
            <dbReference type="ChEBI" id="CHEBI:20489"/>
        </cofactor>
        <text evidence="12">Contains 1 lysine tyrosylquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Secreted</location>
            <location evidence="2 12">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="12">The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the lysyl oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.13" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A673BEB3"/>
    <dbReference type="Ensembl" id="ENSSORT00005039718.1">
        <property type="protein sequence ID" value="ENSSORP00005038723.1"/>
        <property type="gene ID" value="ENSSORG00005017995.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BEB3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004720">
        <property type="term" value="F:protein-lysine 6-oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050912">
        <property type="entry name" value="LOX-like_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001695">
        <property type="entry name" value="Lysyl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019828">
        <property type="entry name" value="Lysyl_oxidase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817:SF10">
        <property type="entry name" value="LYSYL OXIDASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01186">
        <property type="entry name" value="Lysyl_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00074">
        <property type="entry name" value="LYSYLOXIDASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00926">
        <property type="entry name" value="LYSYL_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 12" id="KW-0886">LTQ</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0964">Secreted</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="7 12" id="KW-0801">TPQ</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025627122" description="Lysyl oxidase homolog" evidence="14">
        <location>
            <begin position="22"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="138"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="255"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="141"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="518" mass="57931" checksum="E538CEC0160C5013" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEY8</accession>
    <name>A0A673BEY8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox protein Hox-A4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hoxa4a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Antp homeobox family. Deformed subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BEY8"/>
    <dbReference type="FunCoup" id="A0A673BEY8">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040179.1">
        <property type="protein sequence ID" value="ENSSORP00005039172.1"/>
        <property type="gene ID" value="ENSSORG00005018309.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BEY8"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000029">
        <property type="entry name" value="Homeobox protein Hox-D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050609">
        <property type="entry name" value="Antp_homeobox_Deformed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771">
        <property type="entry name" value="HOMEOTIC PROTEIN DEFORMED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="147"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="149"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="248" mass="27842" checksum="129A24C23575E372" modified="2020-06-17" version="1">MTMSSYLINSNYIEPSFPPCDEYQQSGYIPNPGDYYERPKDTGFPHHDEPSYPRSNYTESGYDYGNVPATGLDDFSDGHHAQPQPVPQSHGPRLTAAPDGGAGANASKDCSLANEVYPGVAKGKEPVVYPWMKKVHVNTVNASYNGGVPKRSRTAYTRQQALELEKEFHFNRYLTRRRRVEIAHTMCLSERQVKIWFQNRRMKWKKEHKLPNTKIRSSSSASSSSSGAQQQQIKTGQQLVPTPCTAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BH11</accession>
    <name>A0A673BH11_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Gasdermin-E-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115427802</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gasdermin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002330.1">
        <property type="nucleotide sequence ID" value="XM_030146470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BH11"/>
    <dbReference type="Ensembl" id="ENSSORT00005042933.1">
        <property type="protein sequence ID" value="ENSSORP00005041861.1"/>
        <property type="gene ID" value="ENSSORG00005019435.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427802"/>
    <dbReference type="InParanoid" id="A0A673BH11"/>
    <dbReference type="OrthoDB" id="8815334at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040460">
        <property type="entry name" value="Gasdermin_pore"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042377">
        <property type="entry name" value="GSDME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207:SF3">
        <property type="entry name" value="DEAFNESS, AUTOSOMAL DOMINANT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207">
        <property type="entry name" value="NONSYNDROMIC HEARING IMPAIRMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04598">
        <property type="entry name" value="Gasdermin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gasdermin pore forming" evidence="4">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="438" mass="48985" checksum="17D416EDA830E118" modified="2020-06-17" version="1">MFSKATANIVRQIDPEGSLIHVSRLNDSQKLFPMALVVKRNRFWAWQRPKYQPTDFSLGDLLQGNEKLSPGVSEEDFLTYSGAHMDRITGKVETQAGPVSATLEGRGSYNLQSSFGKLKKKELDVKRLVRDSETRMVNMQHMLMQQLEKKAEVLAVVKDIVITAESCSIKQTKKEQCALQGVLGLVGLLGSFLKVCVSDRSSIHLDSDESLEIPPGTVVAYSVRELEIKSNGHFMICLQPGTIGGIESDSVSHWNSHDSLNVVDGRINDSQERDLFPLAELPQSTRSELFKKLQDTMKDKYALSMFQSALEESRLEDTSDVPAHLNAIHLLVSAMEELPDETLNLLSKSHPDFLEAFNMLIFGLKESCLHLPVQSLPVSLKDNQNFQLAEQLLLSTDVTLTRRNDELWAETKTDPQVLPLVLCLSVHGLTLLCKEQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BHA7</accession>
    <name>A0A673BHA7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Breakpoint cluster region protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">si:dkey-91m11.5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005041038.1">
        <property type="protein sequence ID" value="ENSSORP00005040002.1"/>
        <property type="gene ID" value="ENSSORG00005014171.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BHA7"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08686">
        <property type="entry name" value="C2_ABR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04387">
        <property type="entry name" value="RhoGAP_Bcr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000057">
        <property type="entry name" value="active breakpoint cluster region-related protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000004">
        <property type="entry name" value="active breakpoint cluster region-related protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.30">
        <property type="entry name" value="Bcr-Abl oncoprotein oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037769">
        <property type="entry name" value="Abr/Bcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015123">
        <property type="entry name" value="Bcr-Abl_oncoprot_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036481">
        <property type="entry name" value="Bcr-Abl_oncoprot_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182:SF3">
        <property type="entry name" value="BREAKPOINT CLUSTER REGION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182">
        <property type="entry name" value="BREAKPOINT CLUSTER REGION PROTEIN BCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09036">
        <property type="entry name" value="Bcr-Abl_Oligo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19057">
        <property type="entry name" value="PH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69036">
        <property type="entry name" value="Bcr-Abl oncoprotein oligomerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="DH" evidence="10">
        <location>
            <begin position="510"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="720"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="902"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="1065"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="73"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="319"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="418"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="378"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1281" mass="144529" checksum="1897566A30049FB8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIA5</accession>
    <name>A0A673BIA5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ift80</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BIA5"/>
    <dbReference type="Ensembl" id="ENSSORT00005043383.1">
        <property type="protein sequence ID" value="ENSSORP00005042306.1"/>
        <property type="gene ID" value="ENSSORG00005019602.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BIA5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000007">
        <property type="entry name" value="Intraflagellar transport 80 homolog (Chlamydomonas)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000298">
        <property type="entry name" value="Intraflagellar transport 80 homolog (Chlamydomonas)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001115">
        <property type="entry name" value="Intraflagellar transport 80 homolog (Chlamydomonas)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056456">
        <property type="entry name" value="Beta-prop_IFT80_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056157">
        <property type="entry name" value="TPR_IFT80_172_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24098:SF11">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24098">
        <property type="entry name" value="OUTER SEGMENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23335">
        <property type="entry name" value="Beta-prop_IFT80_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23387">
        <property type="entry name" value="TPR_IFT80_172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="IFT80 second beta-propeller" evidence="6">
        <location>
            <begin position="300"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="IFT80/172/WDR35 TPR" evidence="7">
        <location>
            <begin position="616"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="780" mass="87645" checksum="B0E9F5A4782FCCE0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BK96</accession>
    <name>A0A673BK96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heat shock protein beta-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Heat shock 27 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hspb1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BK96"/>
    <dbReference type="FunCoup" id="A0A673BK96">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044142.1">
        <property type="protein sequence ID" value="ENSSORP00005043051.1"/>
        <property type="gene ID" value="ENSSORG00005019917.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BK96"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06475">
        <property type="entry name" value="ACD_HspB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000024">
        <property type="entry name" value="heat shock protein beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037876">
        <property type="entry name" value="ACD_HspB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF7">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SHSP" evidence="14">
        <location>
            <begin position="93"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="214" mass="24173" checksum="6587DF3C75A06C7B" modified="2020-06-17" version="1">MAERRIPFTLLRTPSWDPFRDWHQSSRIFDQMFGMPALPEDFTTFPSTHWPGYLRPSILAPDMAAMMPQSPLMYQSPMMHTSNPMMAAQQARALSRQLSSGMSEIKQTQDSWKVSLDVNHFSPEELVVKTKDGVVEITGKHEERKDEHGFVSRCFTRKYTLPPTANVEKVTSSLSPEGFLTVEAPLIRPAIESAETSIPVTVDTKSVASKFPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLW7</accession>
    <name>A0A673BLW7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Neurexin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">nrxn1a</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Neuronal cell surface protein that may be involved in cell recognition and cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005042644.1">
        <property type="protein sequence ID" value="ENSSORP00005041577.1"/>
        <property type="gene ID" value="ENSSORG00005019143.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLW7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000015">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000029">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000001">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000003">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000004">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000005">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000007">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF51">
        <property type="entry name" value="NEUREXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025631738" evidence="16">
        <location>
            <begin position="20"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1392"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="29"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="202"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="257"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="461"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="659"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="701"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="888"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1066"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="17">
        <location>
            <begin position="1107"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1313"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1432"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1315"/>
            <end position="1351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054347"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1465" mass="160696" checksum="0FC758C0D7231AD9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BS33</accession>
    <name>A0A673BS33_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nfkbiz</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BS33"/>
    <dbReference type="Ensembl" id="ENSSORT00005046756.1">
        <property type="protein sequence ID" value="ENSSORP00005045601.1"/>
        <property type="gene ID" value="ENSSORG00005020951.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BS33"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NF-kappa-B inhibitor zeta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
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    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF5">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
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    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="210"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="344"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="380"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="445" mass="49887" checksum="57EA42682A99FA91" modified="2020-06-17" version="1">MSSLKDMIFDTLRPFLFCAHLCVFNCGLDFIITDPRPSVHRRNTVKELLMLRRQQTQNNNLLLYLFQKWKCSEEDNIPEHKLKYTRSEAFPNDSSLQPLTTPLAVNPNIPDYVLNAAPGNSLFTSQQLQYPSLLQGQAAPTVEVKMTLFNWQIQQEAMKMEGVSPEQLNMQDADGDTFLHIAVAQGRRALAYVLAAKMGLNGSLDIKEHNGQTALQIAAATNQHLIVSDLLVCGAKVNTQDLWGRSPLHVCAEKGYFPCLQSICRTLTETGQPLVVEMFNYDGLTPLHAAVLSHNAVIKDLRSLDKSTTCMATERQVKRRYMYLECIKTLLHMGASCETKERKSGRTCLHMAAEEANMELLHLFLEQPSFLSLVKQKTFSGNTALHIVSSLQNHKAQVEAVRLLMRKGADPGSRNSENELPSQLVPEGPTGEKVRRILKGKYVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BS96</accession>
    <name>A0A673BS96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alpha-mannosidase</fullName>
            <ecNumber evidence="17">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">man2a2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 H2O = 2 alpha-D-mannopyranose + an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:56052"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28729"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="3.2.1.114"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_029991744.1">
        <property type="nucleotide sequence ID" value="XM_030135884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BS96"/>
    <dbReference type="Ensembl" id="ENSSORT00005045795.1">
        <property type="protein sequence ID" value="ENSSORP00005044670.1"/>
        <property type="gene ID" value="ENSSORG00005020455.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420549"/>
    <dbReference type="CTD" id="4122"/>
    <dbReference type="InParanoid" id="A0A673BS96"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004572">
        <property type="term" value="F:mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000009">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF57">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 17" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="19">
        <location>
            <begin position="502"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1152" mass="131141" checksum="9A7C5E556F6117E2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BT62</accession>
    <name>A0A673BT62_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member E2B</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BT62"/>
    <dbReference type="Ensembl" id="ENSSORT00005044888.1">
        <property type="protein sequence ID" value="ENSSORP00005043777.1"/>
        <property type="gene ID" value="ENSSORG00005020199.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BT62"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="11"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="153" mass="17670" checksum="C70551FEDC6ADE7F" modified="2020-06-17" version="1">ESRLWNRCSLAYMCLWDFFSFCTLFLNKYILSLLEGEPSMLGAVQMLSTTVIGCVKMLVPCCLYQHKSRTKYHTNLTMIMLNNYFPFVSLRFITVVLGLLSLKNVVVSFAEMVKSSAPMFTVVMSRLILGEYTGSISYKVHLVICYFNQFKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVC0</accession>
    <name>A0A673BVC0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Armadillo repeat containing 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">odad2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BVC0"/>
    <dbReference type="FunCoup" id="A0A673BVC0">
        <property type="interactions" value="592"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005048007.1">
        <property type="protein sequence ID" value="ENSSORP00005046836.1"/>
        <property type="gene ID" value="ENSSORG00005021425.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVC0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 4 ARMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46241:SF1">
        <property type="entry name" value="OUTER DYNEIN ARM-DOCKING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="737"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="862"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1026" mass="114053" checksum="2B8FFB90D12199BA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYL6</accession>
    <name>A0A673BYL6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger CCCH domain-containing protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">zc3h10</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Specific regulator of miRNA biogenesis. Binds, via the C3H1-type zinc finger domains, to the binding motif 5'-GCAGCGC-3' on microRNA pri-MIR143 and negatively regulates the processing to mature microRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029990144.1">
        <property type="nucleotide sequence ID" value="XM_030134284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BYL6"/>
    <dbReference type="FunCoup" id="A0A673BYL6">
        <property type="interactions" value="1414"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047287.1">
        <property type="protein sequence ID" value="ENSSORP00005046129.1"/>
        <property type="gene ID" value="ENSSORG00005021134.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419483"/>
    <dbReference type="CTD" id="84872"/>
    <dbReference type="InParanoid" id="A0A673BYL6"/>
    <dbReference type="OrthoDB" id="250836at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000003">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF6">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="361"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="10"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="463"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="551" mass="56431" checksum="F7A76A2802AF656C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C013</accession>
    <name>A0A673C013_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP-ribosylhydrolase ARH3</fullName>
            <ecNumber evidence="7">3.2.1.143</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ADP-ribose glycohydrolase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ADP-ribosylhydrolase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">O-acetyl-ADP-ribose deacetylase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Poly(ADP-ribose) glycohydrolase ARH3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">[Protein ADP-ribosylarginine] hydrolase-like protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">[Protein ADP-ribosylserine] hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">adprs</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>alpha-NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68792"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:77017"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="25">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="25">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673C013"/>
    <dbReference type="FunCoup" id="A0A673C013">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047755.1">
        <property type="protein sequence ID" value="ENSSORP00005046594.1"/>
        <property type="gene ID" value="ENSSORG00005021329.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C013"/>
    <dbReference type="OrthoDB" id="410104at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140290">
        <property type="term" value="P:peptidyl-serine ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4080.10:FF:000001">
        <property type="entry name" value="ADP-ribose glycohydrolase ARH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF24">
        <property type="entry name" value="ADP-RIBOSYLHYDROLASE ARH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 25" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="357"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="365"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="391" mass="41677" checksum="94EEFE984BC54CCC" modified="2020-06-17" version="1">MAMTAARAVAAGGPASLSRFRGALVAGVLGDCVGGEFEGAEEVPMERVLQHLNSLDDETKGNGILDYSDDTAMARCVVQSLLTRTGYDEQDMARRFGKEFSTSPGRGYGSGVVQVLKKLSAPQLSDVYQPARAQFNGRGSFGNGGAMRAAPFALAFPDLADVKRFAGLGAMLTHSCSLGYNGAILQALAVHLALQGALDPPQQFINRLIAEMEEVEDNEVTRNDARILKEAEKPFCERLHRVRDLIDRSKVSIEEVISELGNGVAALHSVPTAIFCVLHCLQPRECLPESYGGLERTIAYSLALGGDTDTIACMAGAIAGAHYGIEAIPQSWIKCCEGAEDADVNAGRLHALYYQSAKGGGSGNENERGEQSCDDRSKNASNGTEKKTRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2G2</accession>
    <name>A0A673C2G2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C2G2"/>
    <dbReference type="Ensembl" id="ENSSORT00005048310.1">
        <property type="protein sequence ID" value="ENSSORP00005047137.1"/>
        <property type="gene ID" value="ENSSORG00005021568.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C2G2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF18">
        <property type="entry name" value="ZONA PELLUCIDA PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="422" mass="46914" checksum="DA4332FCA7BE386D" modified="2020-06-17" version="1">MDYWYQLLSIPSWTSTTESNIFKRGQVVHLQVSANTRPDEHLFVLSCFVSASPEPHTKPKHAVIINKGCAAPLDSSHAVAQFVDSDREDVVNLALNTSFITSEFYIHCSVLVSNQSNTSGSKSCNYNFIQWEDLSGNTDVCKCCSSKCKSLSLKHFPHDATAVVSIGPLFIEYEITSSIHVADSLQSSPAETEDKIISGASFTNRFSDSSQFFSPESVVNQGQVIRLTVWLPGETKDTEHQKIDSEPEDDLTAELQQSDVESNHILDLPPLSTDQEISLNAPTNEIKAGNEQGDALPWDVNALTLFDGRPIPPHLEEAAIEEESQRKKRCEKVEALPLPGEVNIDDMEMLAQKQTEVCEERNYVQPVIRSKIQFSRSVDGSQALSYEEEVMQEEEGMGDIRNLGMHGFKKGGLLSTFLDLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3N7</accession>
    <name>A0A673C3N7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">SAP30 like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sap30l</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP30 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C3N7"/>
    <dbReference type="FunCoup" id="A0A673C3N7">
        <property type="interactions" value="1225"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051169.1">
        <property type="protein sequence ID" value="ENSSORP00005049966.1"/>
        <property type="gene ID" value="ENSSORG00005022669.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C3N7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1800.30:FF:000001">
        <property type="entry name" value="Histone deacetylase complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024145">
        <property type="entry name" value="His_deAcase_SAP30/SAP30L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025717">
        <property type="entry name" value="SAP30_zn-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286:SF6">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP30L-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286">
        <property type="entry name" value="SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13866">
        <property type="entry name" value="zf-SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 zinc-finger" evidence="12">
        <location>
            <begin position="22"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 Sin3 binding" evidence="13">
        <location>
            <begin position="110"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20603" checksum="AB5E3AA89191C5B4" modified="2020-06-17" version="1">MMNGFSTEEDSHDGPPAPPFYGQSCCLIEDGERCGRPAGNASFSKRIQKSISQKKLKLDIDKSVRHLYICDFHKNFIQSVRNKRKRKTSDDGGESPDHDVEVPEVDLFQLQVNTLRRYKRHYKLQTRPGLNKAQLAETVSRHFRNIPVNEKETLTYFIYMVKSSKSRLDQKADGSKPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3Y0</accession>
    <name>A0A673C3Y0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Musashi RNA-binding protein 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Musashi family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C3Y0"/>
    <dbReference type="Ensembl" id="ENSSORT00005049135.1">
        <property type="protein sequence ID" value="ENSSORP00005047949.1"/>
        <property type="gene ID" value="ENSSORG00005021878.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C3Y0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12323">
        <property type="entry name" value="RRM2_MSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000025">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034126">
        <property type="entry name" value="MSI_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF10">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="21"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="110"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="385" mass="41851" checksum="EC78F5070E8FF345" modified="2020-06-17" version="1">DADMEADGGARSPADPQHDPGKMFVGGLSWQTSPDNLRDYFNKFGEIRECMVMRDPTTKRSRGFGFITFTESSSVDKVLAHPHHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSANTVVEDVKQYFQQFGKVEDAMLMFDKTTNRHRGFGFVTFEVEAVVEKVCGIHFHEINNKMVSDVPPGSRGRPRAPPYAVDAFMLGMGMLSCPNIVATYSRGYAGFSPNYSYQFTGEISDPNSKQQRCPPPPLWFYPGFSDYGVYFSAVGGPDVRGGASYSLADYATLGPQTAAPVLPSEQPGSACTSPSSVQHLPSPDPFKSPGVFQPFATRVCFPGPGSPGPVAELYGPTGQDFGGGTLHQHQQPQPGSAFSPHVGGQLMTSCWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C759</accession>
    <name>A0A673C759_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Bradykinin receptor B2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C759"/>
    <dbReference type="Ensembl" id="ENSSORT00005051028.1">
        <property type="protein sequence ID" value="ENSSORP00005049825.1"/>
        <property type="gene ID" value="ENSSORG00005022608.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004947">
        <property type="term" value="F:bradykinin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000496">
        <property type="entry name" value="Brdyknn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF957">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00425">
        <property type="entry name" value="BRADYKININR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="44"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="340" mass="38758" checksum="AE17F4ABDE8D36E0" modified="2020-06-17" version="1">MDKEETGYTQIKQSNQCPETETDQMIYTVVPVYVLIISVLGIVLNILVLLVFWLHKKACTVPEIYLSNMAAADLLLMCFMPFWAVTIANKYNWFFGPHMCRLVNLCIVVNATCSIYFLVLVSIDRFLALAHPLIRSRKCAQLGCVLVWCFGLILGIPTLICRKVKDYPETNSSECSFGCSPNVFYTNEGLQTVFGFIIPVAIISCCTVKIIQALKNRPVEGARIQKVEHKATFLVLVVLVAFLICWVPFHIMRMIEIFLRTGIIPGNSSCEVVRVFDICMHTFNYFAFFNSVLNPILYVIVGKNFRTKMREVSKRYTETKSSSTRLTTKHKYVQVCIFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CA76</accession>
    <name>A0A673CA76_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC-type glutathione-S-conjugate transporter</fullName>
            <ecNumber evidence="12">7.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">abcc6a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.3" evidence="12"/>
    <dbReference type="FunCoup" id="A0A673CA76">
        <property type="interactions" value="604"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052146.1">
        <property type="protein sequence ID" value="ENSSORP00005050930.1"/>
        <property type="gene ID" value="ENSSORG00005017917.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CA76"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000293">
        <property type="entry name" value="ATP binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000032">
        <property type="entry name" value="ATP-binding cassette sub-family C member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005292">
        <property type="entry name" value="MRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00957">
        <property type="entry name" value="MRP_assoc_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF339">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="945"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="997"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1080"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="315"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="614"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="957"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1272"/>
            <end position="1506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024220"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1510" mass="169383" checksum="D6582A0CA6DC4AD9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CDD1</accession>
    <name>A0A673CDD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 66 member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">slc66a3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029983485.1">
        <property type="nucleotide sequence ID" value="XM_030127625.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MannP-dilichol_defect-1"/>
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        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF3">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 66 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023381">
        <property type="entry name" value="MannP-dilichol_defect-1p"/>
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    </dbReference>
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        <property type="entry name" value="CTNS"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3 5 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="60"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
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        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR023381"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="205" mass="23429" checksum="417D38188A314D72" modified="2020-06-17" version="1">MESDTLLHMANFSTLFVCMVLKFPQIFVLMRAKSTAGVSLNSLLLELTGFIVFVTYQMYYDYPPPTYLEYPILIAQDVILLLLILHYNGSLQQSLIYTLVFVGGWRLLTVEKWIIDLAMSLCTFISAASKFAQLQCLWRSKDAGQVSALSWAMATYTCMARIYTTTVTTGDMQVLVRFIVMTSLNMWVLLTILYYQKHSSSKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CES4</accession>
    <name>A0A673CES4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">SNF-related serine/threonine-protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">SNF1-related kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">snrka</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">May play a role in hematopoietic cell proliferation or differentiation. Potential mediator of neuronal apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673CES4"/>
    <dbReference type="FunCoup" id="A0A673CES4">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051818.1">
        <property type="protein sequence ID" value="ENSSORP00005050603.1"/>
        <property type="gene ID" value="ENSSORG00005022935.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CES4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14074">
        <property type="entry name" value="STKc_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14339">
        <property type="entry name" value="UBA_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000166">
        <property type="entry name" value="SNF-related serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF90">
        <property type="entry name" value="SNF RELATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="16"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="23">
        <location>
            <begin position="291"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="405"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="521"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="746"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="536"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="579"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="610"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="818" mass="88317" checksum="37E5CE80DD7CCEDB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CH21</accession>
    <name>A0A673CH21_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Chromobox protein homolog 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cbx8a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
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            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CH21"/>
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    <dbReference type="InParanoid" id="A0A673CH21"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CD_polycomb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033773">
        <property type="entry name" value="CBX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
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    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF1">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17218">
        <property type="entry name" value="CBX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="7">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="129"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="367" mass="42269" checksum="5A2231FB008DAEED" modified="2020-06-17" version="1">MELSAVGESVFAAESIIKRRIRRGRWEYLVKWKGWSQKYSTWEPEENILDARLFSAFEERERERELFGPKKRGPKPETFLLKAKAKAKDKTYEFRREPPRGIQVSYPIPEPMITPRAREGLRTVVPTIFPPSAINRGESVHMRPPEPERRPRPSPPASLMDQESIRLPKKRGRKPKLHLHCVEDHGSGSTEPDSKRSRLLEEPHDLSTMPRRMHHHGETSDHSLIQLTKRFQEETTITPKSSSGQRHMGASGLSYTCSLSSDVRKGERGKHRTMCTSRMSIPQPGKMKHPCEHRGHQVQQCCLQREQPDIIPRFSDSVIDSSTLPSSSWKPTFTNLDTVTVTDVTMNFLTVTIRESNTDKGFFRDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJ70</accession>
    <name>A0A673CJ70_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nuclear factor I/C</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Binds DNA as a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJ70"/>
    <dbReference type="Ensembl" id="ENSSORT00005056926.1">
        <property type="protein sequence ID" value="ENSSORP00005055641.1"/>
        <property type="gene ID" value="ENSSORG00005024825.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJ70"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000647">
        <property type="entry name" value="CTF/NFI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020604">
        <property type="entry name" value="CTF/NFI_DNA-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019739">
        <property type="entry name" value="CTF/NFI_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019548">
        <property type="entry name" value="CTF/NFI_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492:SF2">
        <property type="entry name" value="NUCLEAR FACTOR 1 C-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492">
        <property type="entry name" value="NUCLEAR FACTOR I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10524">
        <property type="entry name" value="NfI_DNAbd_pre-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00349">
        <property type="entry name" value="CTF_NFI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51080">
        <property type="entry name" value="CTF_NFI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CTF/NF-I" evidence="9">
        <location>
            <begin position="5"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="262" mass="30321" checksum="5BB0E9E858CAC48F" modified="2020-06-17" version="1">MIYFSLCVFSVLQDEFHPFIEALLPQVRAFAYTWFNLQARKRKYFKKHEKRMTKEEERAVKDELLGEKAEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAACCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKGGQCSNPVLCVQPRHISVSVKELDLYLAYYVQERGTSQHPLCVFPHVIIKGFHTTESGPRFMFLLYCIHLIQLVLVSHCDFASAQTASGYRQIELCVLCVFIQLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLX6</accession>
    <name>A0A673CLX6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Hephaestin</fullName>
            <ecNumber evidence="3">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">cp</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Plasma membrane ferroxidase that mediates the extracellular conversion of ferrous/Fe(2+) iron into its ferric/Fe(3+) form. Couples ferroportin which specifically exports ferrous/Fe(2+) iron from cells to transferrin that only binds and shuttles extracellular ferric/Fe(3+) iron throughout the body. By helping iron transfer from cells to blood mainly contributes to dietary iron absorption by the intestinal epithelium and more generally regulates iron levels in the body.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:11149"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="23">Part of a complex composed of SLC40A1/ferroportin, TF/transferrin and HEPH/hephaestin that transfers iron from cells to transferrin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Basolateral cell membrane</location>
            <topology evidence="20">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673CLX6"/>
    <dbReference type="Ensembl" id="ENSSORT00005056478.1">
        <property type="protein sequence ID" value="ENSSORP00005055200.1"/>
        <property type="gene ID" value="ENSSORG00005024655.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CLX6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000015">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000033">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000002">
        <property type="entry name" value="Hephaestin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024715">
        <property type="entry name" value="Factor_5/8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF226">
        <property type="entry name" value="CERULOPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000354">
        <property type="entry name" value="Factors_V_VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 26" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025543181" description="Hephaestin" evidence="26">
        <location>
            <begin position="20"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="28">
        <location>
            <begin position="94"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="27">
        <location>
            <begin position="265"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="28">
        <location>
            <begin position="817"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="27">
        <location>
            <begin position="967"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="278"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="546"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="648"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054029"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00065139"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000354-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1078" mass="123057" checksum="3918AE0B260455B2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMR0</accession>
    <name>A0A673CMR0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Type-1 angiotensin II receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">agtr1b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for angiotensin II, a vasoconstricting peptide, which acts as a key regulator of blood pressure and sodium retention by the kidney. The activated receptor in turn couples to G-alpha proteins G(q) (GNAQ, GNA11, GNA14 or GNA15) and thus activates phospholipase C and increases the cytosolic Ca(2+) concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Receptor for angiotensin II, a vasoconstricting peptide, which acts as a key regulator of blood pressure and sodium retention by the kidney. The activated receptor in turn couples to G-alpha proteins G(q) and thus activates phospholipase C and increases the cytosolic Ca(2+) concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
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    </dbReference>
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        <property type="gene ID" value="ENSSORG00005024452.1"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:external side of plasma membrane"/>
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    </dbReference>
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        <property type="term" value="F:angiotensin type I receptor activity"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ANGIOTENSINR"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
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        <location>
            <begin position="62"/>
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            <end position="223"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
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        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="40501" checksum="891E87A3E22A6AA2" modified="2020-06-17" version="1">MQNITAEETKGMNLTCGLSGRHQFVFTVVPIVYGCNFVIGIVGNSMVVAVIYCYIKLKTVANIFVLNLAVSDLTFLITLPMWATFTASGYHWPFGEFLCKTSAGLAIFNLYTSIFFLTALSIDRYLAIVHPMRSRRFRTVMYAWITCVLIWVFAFLLSVPIVLTREVHNFSNITMCVILHPSTVNYSLQELLLAISLMKSLLGFLVPFIIIITCYCLIVRALLRARHIQKSSRSRDDEVLRMLAAAVLAFFLCWVPHQIFHFIQLLIQLKVVENCGIEDIVDTAMPFTICIAYFNSCVNPIVYGYVGKNFRKNLLRLLRCSPNKATGPHPSISSKMSALSFRASEALSLTVKSKASSDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CP02</accession>
    <name>A0A673CP02_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like protein 38</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">klhl38b</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
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    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="34"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="584" mass="67010" checksum="ECA20B1D0DB97A11" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CP17</accession>
    <name>A0A673CP17_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Copine Va</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cpne5a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CP17"/>
    <dbReference type="Ensembl" id="ENSSORT00005058692.1">
        <property type="protein sequence ID" value="ENSSORP00005057376.1"/>
        <property type="gene ID" value="ENSSORG00005025452.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CP17"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000099">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF51">
        <property type="entry name" value="COPINE-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="252"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="502" mass="55983" checksum="E6B951D743875E40" modified="2020-06-17" version="1">MDKVEATGTVRDFDPISGSIPATKVEITVSCRLFSKLKSLLHVSHLFLIQFGRTEVIDNTLNPDFVRKYILDYFFEEKQNLRFDVYDIDSKSPDLAKHDFLGQVHCTLGEIVGSPASRLEKTLGCGKPQPGIPGKKCGTIILSAEELGNCRDYATMQFCAHKLDKKDFFGKSDPFMVFYRSNEDGTFTICHKTEVVKNTLNPVWQPFSIPVRALCNGDYDSHDFIGEFTTSYKELCRGQNQLNVYEVSETVDIIISLFGNPSQSTSLHYMNPYQMNAYAMALKAVGEIIQDYDSDKMFPALGFGAKLPPDGRVSHEFPLNGNIENPYCNGMEGILEAYHQSLKTVQLYGPTNFAPVVNHVARYAAAVQDGSQYFVLLIITDGVISDMAQTKEAIVNAAKLPMSIIIVGVGQAEFDAMVELDGDDIRISSRGKLAERDIVQFVPFRDYMDRTGNHVLSMARLAKDVLAEIPDQLISYMKSRAIKPNPVPPVYVPVDHPEPNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CU30</accession>
    <name>A0A673CU30_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prostasin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">zgc:92313</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CU30"/>
    <dbReference type="Ensembl" id="ENSSORT00005057400.1">
        <property type="protein sequence ID" value="ENSSORP00005056107.1"/>
        <property type="gene ID" value="ENSSORG00005024987.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CU30"/>
    <dbReference type="OrthoDB" id="546450at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000024">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF144">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE PROTEASE CTRL-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025464136" evidence="8">
        <location>
            <begin position="23"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="36"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="309" mass="33759" checksum="30FF64FEEDD4C93B" modified="2020-06-17" version="1">MSRDFQFSAIFWCATGILVVVCAEECGRPMIVENRIVGGIDATDGAWPWQVDIQTNNDGHICGGSLITNKWVLSAAHCFPNPYDVSPYIVYAGRYQLNNFNMHQSVHRVSRIVIPAGYEEPHNGKDLALVQLSSPVTWSDYIRPVCLPASGTLFPGGMRCYVTGWGNVRDEVPLSGVGTLQEVQVPIISQSSCQEMYNLNPTEQVDILYDMICAGYQEGGKDSCQGDSGGPLVCQMVNGTWVQAGVVSFGLGCAHRNQPGVYAKVSAYSDFIRNTIPEIRLYGSANRNWCGSTAMLVGCLSTLLTLLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CV88</accession>
    <name>A0A673CV88_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein cereblon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">crbn</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRBN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989860.1">
        <property type="nucleotide sequence ID" value="XM_030134000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CV88"/>
    <dbReference type="FunCoup" id="A0A673CV88">
        <property type="interactions" value="1441"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059653.1">
        <property type="protein sequence ID" value="ENSSORP00005058330.1"/>
        <property type="gene ID" value="ENSSORG00005025749.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419302"/>
    <dbReference type="CTD" id="51185"/>
    <dbReference type="InParanoid" id="A0A673CV88"/>
    <dbReference type="OrthoDB" id="267517at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd15777">
        <property type="entry name" value="CRBN_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000004">
        <property type="entry name" value="Cereblon, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000002">
        <property type="entry name" value="Cereblon, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.40:FF:000002">
        <property type="entry name" value="Cereblon, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034750">
        <property type="entry name" value="CULT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF4">
        <property type="entry name" value="PROTEIN CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51788">
        <property type="entry name" value="CULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="13">
        <location>
            <begin position="88"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="CULT" evidence="14">
        <location>
            <begin position="326"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="453" mass="51434" checksum="1ED0AB001DFA9735" modified="2020-06-17" version="1">MADERGGGEDNINDNMGNQIQLLPDNEEEEEEEEEDDMETEDRDSEEAEKPNTITFDPSLPTSHAYLGSDMEEFHGRTVHDDDSCQTIPVLPHTAVMLVPGQTLPLQLFRPQEVSMMRSVIQRDRTFAVLAHSDAGDPEAEFGTTAEIYAYREEVEYGIETVKVKAVGRQRFKVHEIRTQADGIRQAKVQILPERILPDPLSAVQLTSLSRLPVQPSSKAPTQSFKQAQCWWSNYQQRKFHCASVTPWPPWVYALYDSESLMNRVKKQLHEWDENLKEDSLPTNAVDFSYRVAACLPIDDALRLQLLKIGSAIQRLRCELDIMDRCTSLCCKQCQDTEITTKNEIFSLSLYGPMAAYVNPHGYVHETLTVYKANNLNLVGRPSTLHSWFPGYAWTIAQCRTCGSHMGWKFTATKKDLSPPRFWGLTRSAMLPRIPQGEGEGEEAREGSRLFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CWN3</accession>
    <name>A0A673CWN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dermatopontin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dpt</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dermatopontin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987303.1">
        <property type="nucleotide sequence ID" value="XM_030131443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CWN3"/>
    <dbReference type="FunCoup" id="A0A673CWN3">
        <property type="interactions" value="1571"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057684.1">
        <property type="protein sequence ID" value="ENSSORP00005056388.1"/>
        <property type="gene ID" value="ENSSORG00005025092.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417492"/>
    <dbReference type="CTD" id="1805"/>
    <dbReference type="InParanoid" id="A0A673CWN3"/>
    <dbReference type="OrthoDB" id="8545119at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026645">
        <property type="entry name" value="Dermatopontin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15040:SF2">
        <property type="entry name" value="DERMATOPONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15040">
        <property type="entry name" value="DERMATOPONTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14704">
        <property type="entry name" value="DERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025516741" description="Dermatopontin" evidence="5">
        <location>
            <begin position="19"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="22821" checksum="0B7F938415B6D628" modified="2020-06-17" version="1">MNRALLVLALSLLATVAAQYHDHHDMQDMDWVNGYRQGFNFQCPHGEVLVAIRSYFSARDGSDRLWSFECQPTPEGLGEPTDCWWDDINRAGMEWTSTCTRNGLVAGVQSKYFESVLDREWQFYCCYYKRRCPYSCMKTTDIPEYYREEGELVIPSYGYFIRGAQTTFSGVLRDRQWKYILCRMTDFDCEFDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3U2</accession>
    <name>A0A672Y3U2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SEC14-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115424699</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029997956.1">
        <property type="nucleotide sequence ID" value="XM_030142096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y3U2"/>
    <dbReference type="Ensembl" id="ENSSORT00005000920.1">
        <property type="protein sequence ID" value="ENSSORP00005000892.1"/>
        <property type="gene ID" value="ENSSORG00005000542.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424699"/>
    <dbReference type="CTD" id="6397"/>
    <dbReference type="InParanoid" id="A0A672Y3U2"/>
    <dbReference type="OrthoDB" id="30289at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039552">
        <property type="term" value="F:RIG-I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039536">
        <property type="term" value="P:negative regulation of RIG-I signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000006">
        <property type="entry name" value="SEC14-like lipid binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
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        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF51">
        <property type="entry name" value="SEC14-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="4">
        <location>
            <begin position="2"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="314"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="3">
        <location>
            <begin position="516"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="710" mass="80919" checksum="BAF7ABA11EB4C96B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y495</accession>
    <name>A0A672Y495_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">N-sulfoglucosamine sulfohydrolase (sulfamidase)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sgsh</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y495"/>
    <dbReference type="FunCoup" id="A0A672Y495">
        <property type="interactions" value="83"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000784.1">
        <property type="protein sequence ID" value="ENSSORP00005000758.1"/>
        <property type="gene ID" value="ENSSORG00005000473.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y495"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016250">
        <property type="term" value="F:N-sulfoglucosamine sulfohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006027">
        <property type="term" value="P:glycosaminoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030200">
        <property type="term" value="P:heparan sulfate proteoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16027">
        <property type="entry name" value="SGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000026">
        <property type="entry name" value="N-sulphoglucosamine sulphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032506">
        <property type="entry name" value="SGSH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF6">
        <property type="entry name" value="N-SULPHOGLUCOSAMINE SULPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16347">
        <property type="entry name" value="SGSH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="6">
        <location>
            <begin position="30"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="N-sulphoglucosamine sulphohydrolase C-terminal" evidence="7">
        <location>
            <begin position="445"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="59885" checksum="1ED9E43258C637B6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4P6</accession>
    <name>A0A672Y4P6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nucleoside diphosphate kinase B</fullName>
            <ecNumber evidence="10">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC115423821</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Major role in the synthesis of nucleoside triphosphates other than ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 23 24">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="10"/>
    <dbReference type="RefSeq" id="XP_029996737.1">
        <property type="nucleotide sequence ID" value="XM_030140877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y4P6"/>
    <dbReference type="Ensembl" id="ENSSORT00005001039.1">
        <property type="protein sequence ID" value="ENSSORP00005001008.1"/>
        <property type="gene ID" value="ENSSORG00005000610.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423821"/>
    <dbReference type="InParanoid" id="A0A672Y4P6"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000039">
        <property type="entry name" value="Nucleoside diphosphate kinase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="22" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="25">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="23">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00013499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="149" mass="17050" checksum="8A237FDC5DF272B0" modified="2020-06-17" version="1">MERTFIAVKPDGVQRGLCGEIIKRLETRGFRLCAAKFMQGSEDFMKNHYLDLKDMPFYAGLCKYMSSGPVLAMVWEGQNIVKLVRMMLGETNPADSKPGSIRGDLCINIGRNIIHGSDTLENAKREVDLWFKAEEFVNWTPCYQPFLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5N2</accession>
    <name>A0A672Y5N2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubby-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tulp3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y5N2"/>
    <dbReference type="Ensembl" id="ENSSORT00005001867.1">
        <property type="protein sequence ID" value="ENSSORP00005001812.1"/>
        <property type="gene ID" value="ENSSORG00005001134.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y5N2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.90.10:FF:000001">
        <property type="entry name" value="Tubby-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005398">
        <property type="entry name" value="Tubby_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF138">
        <property type="entry name" value="TUBBY-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16322">
        <property type="entry name" value="Tub_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01574">
        <property type="entry name" value="TUBBYPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01200">
        <property type="entry name" value="TUB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Tubby N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Tubby N-terminal" evidence="9">
        <location>
            <begin position="59"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Tubby C-terminal" evidence="8">
        <location>
            <begin position="206"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="84"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="456" mass="51100" checksum="7FB77AFC072D2A90" modified="2020-06-17" version="1">MSIERRALLEQKQRRKRQEPLMVQPNTETRPRRSRARRGEEQAPLVESQMSITNDAIMDGIDGPAAFLGSEAPDLGTKIQILSVSQSQPQSQPPNQLQSQLQSQPQSPAAEEPERDGDTETLIEPKTDIHELLQKQGLSGSMNFDEASEHEDDAEQGQTRPLSPNTDTTRPASAASGKDIPELVTPGSPTAESSLIDLANLEEFVLRPAPRGATVKCRITRDKKGMDRGLYPTYFMHMEREDGKRVFLLAGRKRKKSKTSNYLISVDATDLSREGESFIGKLRSNLMGTKFTVYDNGTNPCKNPGTLLEESNTRQELAAICYETNVLGFKGPRKMTVIIPGMNMNFERVPVRPQNEQESLLSRWQNHTLDSLIELHNKAPVWNDDTQSYVLNFHGRVTQASVKNFQIVHDNDPDYIVMQFGRVAEDIFTLDYNYPMCALQAFAIGLSSFDSKLACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y678</accession>
    <name>A0A672Y678_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Zinc finger and BTB domain containing 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">zbtb10</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030002835.1">
        <property type="nucleotide sequence ID" value="XM_030146975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y678"/>
    <dbReference type="FunCoup" id="A0A672Y678">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002149.1">
        <property type="protein sequence ID" value="ENSSORP00005002092.1"/>
        <property type="gene ID" value="ENSSORG00005001275.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428134"/>
    <dbReference type="CTD" id="65986"/>
    <dbReference type="InParanoid" id="A0A672Y678"/>
    <dbReference type="OrthoDB" id="4845755at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000492">
        <property type="entry name" value="Zinc finger and BTB domain containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000045">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF24">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
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        <source>
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        </source>
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</entry>
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            <taxon>Neoteleostei</taxon>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001429.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBU3</accession>
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            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
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        </reaction>
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            <name>Mg(2+)</name>
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    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A672YBU3"/>
    <dbReference type="Ensembl" id="ENSSORT00005000985.1">
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    <dbReference type="InParanoid" id="A0A672YBU3"/>
    <dbReference type="Proteomes" id="UP000472271">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="191"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="113"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="633" mass="70477" checksum="11210D803F1F04F5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YC46</accession>
    <name>A0A672YC46_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial carrier homolog 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mtch2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YC46"/>
    <dbReference type="FunCoup" id="A0A672YC46">
        <property type="interactions" value="2081"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000202.1">
        <property type="protein sequence ID" value="ENSSORP00005000186.1"/>
        <property type="gene ID" value="ENSSORG00005000127.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YC46"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780">
        <property type="entry name" value="MITOCHONDRIAL CARRIER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780:SF20">
        <property type="entry name" value="MITOCHONDRIAL CARRIER HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="119"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="33678" checksum="03CEA0825D07EFE2" modified="2020-06-17" version="1">MADTCGQVLLGSGLTVLSHPLMYIKVLVQVGHEPLPPSLGRNLFGRQVYQLPGLFAYAKHIIKIDGKAGLFKGLGPRLCAGTIGTVVHSKVVQVIQNSLHFHHHLNCSMETYCVSLTYISFFSQTTKEMIARSCATIVTHPFHVITLRCMVQFIGRETKYSGVFDSIVTVYREEGILGFFAGLIPRLLGDVFALWICNLLAHVINTYAIDDSMSHTGEIKNCSQAVTGFFASMLTYPFVLVSNLMAVNNCGLAGGLPPYASVYPTWVDCWRHLSREGNMSRGNSLFFRKLPAGKTYAIDQKRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCG2</accession>
    <name>A0A672YCG2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YCG2"/>
    <dbReference type="Ensembl" id="ENSSORT00005001369.1">
        <property type="protein sequence ID" value="ENSSORP00005001330.1"/>
        <property type="gene ID" value="ENSSORG00005000782.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCG2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599:SF3">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="3">
        <location>
            <begin position="90"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="562" mass="65173" checksum="BD0936774873C9D1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIZ7</accession>
    <name>A0A672YIZ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Centromere protein I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cenpi</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-I/CTF3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YIZ7"/>
    <dbReference type="FunCoup" id="A0A672YIZ7">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005004663.1">
        <property type="protein sequence ID" value="ENSSORP00005004529.1"/>
        <property type="gene ID" value="ENSSORG00005002746.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YIZ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000939">
        <property type="term" value="C:inner kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mitotic sister chromatid segregation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CENTROMERE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48208:SF2">
        <property type="entry name" value="CENTROMERE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="733" mass="83002" checksum="1A7A51ABB6C6CA32" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ75</accession>
    <name>A0A672YJ75_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJ75"/>
    <dbReference type="Ensembl" id="ENSSORT00005004762.1">
        <property type="protein sequence ID" value="ENSSORP00005004626.1"/>
        <property type="gene ID" value="ENSSORG00005002816.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:adaptive immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="101" mass="11445" checksum="BA4B5CD4B4D87455" modified="2020-06-17" version="1">QTLTESESAVKSPGESHRLTCSTSGFTFRDYAMHWIRQAPGKGLERIAFISQPSGTDTYYSQSVQGRFTISRDNGRNQLYLQMNSLKTEDSAVYYCARHPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ96</accession>
    <name>A0A672YJ96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJ96"/>
    <dbReference type="Ensembl" id="ENSSORT00005004760.1">
        <property type="protein sequence ID" value="ENSSORP00005004624.1"/>
        <property type="gene ID" value="ENSSORG00005002815.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJ96"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003074">
        <property type="entry name" value="Immunoglobulin heavy variable 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="96" mass="10884" checksum="0450303CF22BDE2A" modified="2020-06-17" version="1">SGPAVKRPGESHRLTCTTSGFTFSSYWMAWIRQAPGKGLEWIAYISSGSGSTTYYYQSFQGRFTISRDNGRNQLYLQMNSLKTEDSAVYYCARDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMG8</accession>
    <name>A0A672YMG8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Nuclear distribution protein nudE-like 1-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115424749</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nudE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029998019.1">
        <property type="nucleotide sequence ID" value="XM_030142159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YMG8"/>
    <dbReference type="FunCoup" id="A0A672YMG8">
        <property type="interactions" value="1133"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006063.1">
        <property type="protein sequence ID" value="ENSSORP00005005816.1"/>
        <property type="gene ID" value="ENSSORG00005003500.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424749"/>
    <dbReference type="InParanoid" id="A0A672YMG8"/>
    <dbReference type="OrthoDB" id="5877028at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007100">
        <property type="term" value="P:mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006964">
        <property type="entry name" value="NUDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF0">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04880">
        <property type="entry name" value="NUDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NUDE" evidence="11">
        <location>
            <begin position="135"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="30"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="340" mass="38248" checksum="072D8190272953C2" modified="2020-06-17" version="1">MDTEMIPKFSSKDEEADYWKTQALKYKKCYQEAQEELQEFQEGSRELEAELEAQLGQAEHRLRDLQTENERLKNEVSNLKEKLEQQYAQSYKQISMLEDDLGQTRSIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEGRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERTTDRMSAPSSPTLDTDKMDSAVQASLSLPATPVGKNIELPFISPKALTNGCGNSSLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQAARKNYSTDNGTLINSNATKFSHSIHTTYFDKTTVNGLDPSPLTSMATTRAVPQPGMLPLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YML7</accession>
    <name>A0A672YML7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 238 like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YML7"/>
    <dbReference type="Ensembl" id="ENSSORT00005006109.1">
        <property type="protein sequence ID" value="ENSSORP00005005861.1"/>
        <property type="gene ID" value="ENSSORG00005003520.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YML7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029365">
        <property type="entry name" value="TMEM238"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613">
        <property type="entry name" value="SI:CH211-232M10.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15125">
        <property type="entry name" value="TMEM238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13314" checksum="0784D3FCAC314B10" modified="2020-06-17" version="1">MDPVAFVGSCLPLFVLALLSDVIGLVLLFVGIFANLRVDGRFYGDFFIYTGSIIIFFSLGFWLLWYLGNVPVQEQEDGSGSDVLHVSYLFHFISLCLHGYMHEVHILSALFSCFMAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRN1</accession>
    <name>A0A672YRN1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aldo-keto reductase family 1, member A1b (aldehyde reductase)</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YRN1"/>
    <dbReference type="Ensembl" id="ENSSORT00005007526.1">
        <property type="protein sequence ID" value="ENSSORP00005007251.1"/>
        <property type="gene ID" value="ENSSORG00005004058.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YRN1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025540998" evidence="4">
        <location>
            <begin position="17"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="100"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="283" mass="32267" checksum="F91005584EE54BFD" modified="2020-06-17" version="1">MLSLVQPCLFLFQALLLRDMNYFAVLNTGRKMPLLGLGTWKSQPGQVMVIWTHWSVNVWYRHIDCAAVYGNEAEIGAFVLVLHQILRRRRCVHHIQAVEQLDYLDLYLIHWPYAFRRGDVNFPRKEDGYHSVRRHPLQGDLGAMEKLVGKGLVRAIGLSNFNSQQIDDILSVATIKPAVLQVESHPYLSQEDLLGHCRDRGLVLTPDEPVVLDEPVIAALAQKYNKSPAQIILRWQTQRGVVTIPKSVTESLFDFTLEAEEMQTISALNKGWRYIVPMIEVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTC6</accession>
    <name>A0A672YTC6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC115410230</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A672YTC6"/>
    <dbReference type="FunCoup" id="A0A672YTC6">
        <property type="interactions" value="536"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008154.1">
        <property type="protein sequence ID" value="ENSSORP00005007871.1"/>
        <property type="gene ID" value="ENSSORG00005002769.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTC6"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF24">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="632"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="665"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="718" mass="81815" checksum="8F8B2B0B21677B7C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZJ8</accession>
    <name>A0A672YZJ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Syntaxin-binding protein 5-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">stxbp5l</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZJ8"/>
    <dbReference type="FunCoup" id="A0A672YZJ8">
        <property type="interactions" value="737"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010244.1">
        <property type="protein sequence ID" value="ENSSORP00005009914.1"/>
        <property type="gene ID" value="ENSSORG00005005403.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZJ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15893">
        <property type="entry name" value="R-SNARE_STXBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000521">
        <property type="entry name" value="syntaxin-binding protein 5-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000664">
        <property type="entry name" value="Lethal2_giant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013577">
        <property type="entry name" value="LLGL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF19">
        <property type="entry name" value="SYNTAXIN-BINDING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08366">
        <property type="entry name" value="LLGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00962">
        <property type="entry name" value="LETHAL2GIANT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Lethal giant larvae homologue 2" evidence="16">
        <location>
            <begin position="211"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="624"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="624"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="641"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="711"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1111" mass="123733" checksum="D6F5FC696EE770DD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0U5</accession>
    <name>A0A672Z0U5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">viml</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z0U5"/>
    <dbReference type="Ensembl" id="ENSSORT00005010570.1">
        <property type="protein sequence ID" value="ENSSORP00005010237.1"/>
        <property type="gene ID" value="ENSSORG00005005551.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0U5"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006821">
        <property type="entry name" value="Intermed_filament_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF5">
        <property type="entry name" value="VIMENTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="455" mass="52726" checksum="243357E69833DE41" modified="2020-06-17" version="1">MSYRSAPHSSYKKMFGGEKSAVRSSYTSRHYSTPVRSTRVSYGVSSAPTIYASKTSRLRSSSAMPRLASENLDFSLSDAINTEFITNRTNEKAQMQSLNDRFASYIEKVRFLEQQNKILLAELEQLRGKGTSRVGDLYEDEMRELRRQVDQLTNEKARVEVHRDNLADDIDRLREKLQDEISQREDAESNMQSFRQDVDNAALARLDLERKVESLQDEINFLKKLHDEEMLELQNQIQQQQHVQVDMEMAKPDLTAALRDVRLQYENLASKNIHESEEWYKSKFADLTEAAARNNDALRVAKQEANDYRRQVQALTCEVDALKGTNESLERQMREMEDNFSMENGGYQDTIGRLEEDIHNMKDEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISSPLPNFSSLNLRETMVDSKPHVETTTTKKVLIKTIETRDGQVINESTQNHDDME</sequence>
</entry>
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    <accession>A0A672Z1W6</accession>
    <name>A0A672Z1W6_9TELE</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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    </organism>
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            <authorList>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF26187"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF26189"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="874" mass="97222" checksum="7B4FFCFD6BA60CAB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3F4</accession>
    <name>A0A672Z3F4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z3F4"/>
    <dbReference type="FunCoup" id="A0A672Z3F4">
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    </dbReference>
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        <property type="gene ID" value="ENSSORG00005005555.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pol_alpha_B_N"/>
    </dbReference>
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        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pol_alpha_B_N"/>
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    </dbReference>
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        <property type="entry name" value="DNA_pol_alph_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha subunit B N-terminal" evidence="9">
        <location>
            <begin position="56"/>
            <end position="99"/>
        </location>
    </feature>
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        <location>
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            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="8">
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            <end position="573"/>
        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="623" mass="69344" checksum="3CC0F51350C8D152" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z529</accession>
    <name>A0A672Z529_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Parapinopsin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">parapinopsina</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z529"/>
    <dbReference type="FunCoup" id="A0A672Z529">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012351.1">
        <property type="protein sequence ID" value="ENSSORP00005011960.1"/>
        <property type="gene ID" value="ENSSORG00005006337.1"/>
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    <dbReference type="InParanoid" id="A0A672Z529"/>
    <dbReference type="OrthoDB" id="2101615at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="2 13" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
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    <keyword evidence="3 13" id="KW-0716">Sensory transduction</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <begin position="143"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
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            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
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        <location>
            <begin position="279"/>
            <end position="298"/>
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    </feature>
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        <location>
            <begin position="44"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="347" mass="38122" checksum="D50E6CF5DF782FFB" modified="2020-06-17" version="1">MEHLALHGNSSRPHSSVHTGFLSQTSYTVLAVIMGVFSVVGIILNLLVIVVTVKHRQLRQPLSYALVNLAVCDLGCAVLGGLPTTVSSAMGYFSFGRVGCVIEGLSVAFFGIASLCTIAVISVERYIVVCYPMGAVLFQTRHAVAGVVLSWVWSFVWNIPPLFGWGSFELEGIKISCAPNWQSRDVGDMSYVIMYFCLCFAGPFLIIMVSYSRLLWTLRQVTKLQVSEAGSTNRVEVQVALMVVVMVLAFLVTWLPYAAMALSVIIDSSLYINPLIATIPVYLAKSSTVFNPIIYIFMNRQFRSYAIPTVLCGWNPWAADTTTSECETTVTPVNKTQKVSPKESLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6E4</accession>
    <name>A0A672Z6E4_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">Tectonin beta-propeller repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z6E4"/>
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        <property type="protein sequence ID" value="ENSSORP00005012168.1"/>
        <property type="gene ID" value="ENSSORG00005006446.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006624">
        <property type="entry name" value="Beta-propeller_rpt_TECPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051513">
        <property type="entry name" value="Tectonin_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250:SF1">
        <property type="entry name" value="TECTONIN BETA-PROPELLER REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06462">
        <property type="entry name" value="Hyd_WA"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="TECPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="38328" checksum="85A510A1E862CB72" modified="2020-06-17" version="1">MPITLLWAVDVYGRVYSLSTAGQRWERADDRLLELKRVTAGKGRCWGIGCDHHVYLNMMPIIYGVCAFQRWNPVDGFTDTLLPTDRWPWSDVTGMNPQPLHSSSSPLPQLGGGKGDWYVDQSCGGEPGWEYAKWNSCVRRRRWIRYRRYIAQGTWAKSRKPPLPLCDISCGGWEMRPLWKIPLPLGRVWFREGIHPRVPEGSGWEEVEVPKEVAQLSAGPGDLLWGLLWDGNLLVRTGLSLDSPTGTSWIEVESPGKEVEALHVAVGASVVWVVTKDYKVWFRRGVNSHNPCGSGWISVGGEMMMVDVGLNDQVKIFPLCYGKNNTLLILPHNKERSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8D7</accession>
    <name>A0A672Z8D7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor CWC22 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Nucampholin homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cwc22</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029980888.1">
        <property type="nucleotide sequence ID" value="XM_030125028.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029980889.1">
        <property type="nucleotide sequence ID" value="XM_030125029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z8D7"/>
    <dbReference type="FunCoup" id="A0A672Z8D7">
        <property type="interactions" value="1659"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014059.1">
        <property type="protein sequence ID" value="ENSSORP00005013646.1"/>
        <property type="gene ID" value="ENSSORG00005006178.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412492"/>
    <dbReference type="CTD" id="57703"/>
    <dbReference type="InParanoid" id="A0A672Z8D7"/>
    <dbReference type="OrthoDB" id="1924287at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000004">
        <property type="entry name" value="pre-mRNA-splicing factor CWC22 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="9">
        <location>
            <begin position="608"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="244"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="560"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="811"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="564"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="818"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="869"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="903"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="949"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="999" mass="115779" checksum="47A3CB0245927C26" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8F4</accession>
    <name>A0A672Z8F4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Monocyte to macrophage differentiation-associated 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115426913</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z8F4"/>
    <dbReference type="FunCoup" id="A0A672Z8F4">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013174.1">
        <property type="protein sequence ID" value="ENSSORP00005012772.1"/>
        <property type="gene ID" value="ENSSORG00005006591.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z8F4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140911">
        <property type="term" value="F:pore-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005744">
        <property type="entry name" value="Hy-lIII"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01065">
        <property type="entry name" value="hlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF107">
        <property type="entry name" value="MONOCYTE TO MACROPHAGE DIFFERENTIATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="239" mass="27717" checksum="80560C757B2CEE56" modified="2020-06-17" version="1">MTRVNQETRFMNNRVPPNKRYQPTEYEHAANCATHALWIIPSLLGSSVLHFHSEDQWERVSAWVYGAGLSSLFIISTLFHTVAWKKSHLRSVEQCFHMCDRMVIYFFIAASYAPWLNLRELGPWACHMRWLVWVMASFGTTYVFFFHERYKIMELICYTVMGVFPALVILSMPEQSGLCELLVGGACYCLGMVFFKSDGIVPFAHAIWHLFVAMGAAIHYYAIWKYLYAQPSNQVQTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZA22</accession>
    <name>A0A672ZA22_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZA22"/>
    <dbReference type="Ensembl" id="ENSSORT00005014128.1">
        <property type="protein sequence ID" value="ENSSORP00005013715.1"/>
        <property type="gene ID" value="ENSSORG00005007061.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZA22"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001249">
        <property type="entry name" value="CTCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000875">
        <property type="entry name" value="zinc finger protein 236 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001049">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000367">
        <property type="entry name" value="Zinc finger protein 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF23">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="26"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="602"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="630"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="716"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
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    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
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        </location>
    </feature>
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        <location>
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        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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    </feature>
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        <location>
            <begin position="904"/>
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        <location>
            <begin position="115"/>
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        <location>
            <begin position="59"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="916"/>
            <end position="925"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="956" mass="108566" checksum="DB70B41537CC5064" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGS7</accession>
    <name>A0A672ZGS7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Somatomedin-B and thrombospondin type-1 domain-containing protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">si:dkey-178e17.3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZGS7"/>
    <dbReference type="Ensembl" id="ENSSORT00005016986.1">
        <property type="protein sequence ID" value="ENSSORP00005016476.1"/>
        <property type="gene ID" value="ENSSORG00005008363.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGS7"/>
    <dbReference type="OrthoDB" id="98591at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039942">
        <property type="entry name" value="SBSPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056801">
        <property type="entry name" value="SBSPON_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044004">
        <property type="entry name" value="TSP1_spondin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20920">
        <property type="entry name" value="RPE-SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20920:SF6">
        <property type="entry name" value="SOMATOMEDIN B AND THROMBOSPONDIN TYPE 1 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25031">
        <property type="entry name" value="SBSPON_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19028">
        <property type="entry name" value="TSP1_spondin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025502572" evidence="4">
        <location>
            <begin position="33"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="5">
        <location>
            <begin position="38"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="263" mass="29105" checksum="8CD1229D2990287E" modified="2020-06-17" version="1">MGAHGWSSARVGFFVFGYLAFLFGLMVPEAEAGCRERESPNCCTGRNNECFEYTRRKTVCYCDTYCQKTRDCCEDYQSVCQISAAIDCVVGSWGPWSPCTSPCGIGSTERSRQVSVPPRNGGTPCPDLKQRRGCFGNNAICSSAKEVAKILPDSFKRNFKDPWRRPHMLMKEEKASYCVYLRVKQASAVCKVKLWSAQLVRERLVCAECQSDAMSKSDRCGGDGLASTRTFWAAASVPGCHGSWVRESSAEGCRCPPHSVLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLL3</accession>
    <name>A0A672ZLL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">togaram1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZLL3"/>
    <dbReference type="Ensembl" id="ENSSORT00005018632.1">
        <property type="protein sequence ID" value="ENSSORP00005018091.1"/>
        <property type="gene ID" value="ENSSORG00005008982.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLL3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF87">
        <property type="entry name" value="CRESCERIN-LIKE PROTEIN CHE-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="191"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="425" mass="46487" checksum="841AC31563B7C443" modified="2020-06-17" version="1">MDQELEGTVKALLHKAGESNAFIRQDVDAALDCMVQHCTPSRSISALLTGGLSHLNAVVRKCTAQHLSNLVEKVGAARLLSGGKDLTDRILPAVTKLAQDSSQEARYFGRRMLLSLSCHPDFDKILEKYIPTKDLPTVRDTVLTLKTKGLGEMPQDNQSARGRRSLPGSGTVRASSLTRDLDSEVIEGRVNICRSQPQSIADKTEYIKQISGLLGSKDFRERIKGIDQLVSDCQHNPNMVFDAFKLRLQESNSKVNLYALESLQKITHLLKDNLSQVVNILVPAIVDNHLNSKNNAIYSAAIGAINALTLNLDNVLLLQPFCTKAQFLSGKAKVDLIEKVADLVTELYPRKPQMVEQKVLPLLWHLLGTSTHSGTIHGRGGSVRGATVNLCQALYAQMGPSLTESAASQPANVHKSFNEVLRTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZMV0</accession>
    <name>A0A672ZMV0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transient receptor potential cation channel, subfamily C, member 4 associated protein a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpc4apa</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZMV0"/>
    <dbReference type="Ensembl" id="ENSSORT00005019087.1">
        <property type="protein sequence ID" value="ENSSORP00005018540.1"/>
        <property type="gene ID" value="ENSSORG00005008359.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZMV0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022162">
        <property type="entry name" value="TRPC4AP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31743:SF1">
        <property type="entry name" value="SHORT TRANSIENT RECEPTOR POTENTIAL CHANNEL 4-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31743">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL 4-ASSOCIATED PROTEIN TCPC4AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12463">
        <property type="entry name" value="DUF3689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="790" mass="90448" checksum="C070685A461EC43B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZNG7</accession>
    <name>A0A672ZNG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZNG7"/>
    <dbReference type="Ensembl" id="ENSSORT00005019314.1">
        <property type="protein sequence ID" value="ENSSORP00005018765.1"/>
        <property type="gene ID" value="ENSSORG00005009236.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZNG7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047271">
        <property type="entry name" value="Ephexin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845:SF2">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="180" mass="21292" checksum="14F5BA3727D30B10" modified="2020-06-17" version="1">MFELMGSEASYLRSLQIAVNHFYVSEALKQALSQMEHHILFSNIQRLMATSERFLMDLELRLGENVFISQVGDIVLQHCPAFHRLYVPYVTNMMYQEALLNQLQQQNKEFMYSLKTLEQDPVCQRQSFKSFLVLPFQRITRIRLILEVGIYIHLNYTIYIFNNTHQRLPAPPQRPSDHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZNV7</accession>
    <name>A0A672ZNV7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SPACA4/bouncer family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZNV7"/>
    <dbReference type="Ensembl" id="ENSSORT00005018629.1">
        <property type="protein sequence ID" value="ENSSORP00005018088.1"/>
        <property type="gene ID" value="ENSSORG00005008980.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZNV7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035036">
        <property type="term" value="P:sperm-egg recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046354">
        <property type="entry name" value="SPACA4/Bouncer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47613">
        <property type="entry name" value="SPERM ACROSOME MEMBRANE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47613:SF1">
        <property type="entry name" value="SPERM ACROSOME MEMBRANE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025571635" description="UPAR/Ly6 domain-containing protein" evidence="10">
        <location>
            <begin position="27"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="11">
        <location>
            <begin position="52"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="132" mass="14765" checksum="CC0105DD31452D23" modified="2020-06-17" version="1">MGSCCVPNMLHVSVLWIYLLLPSLLCENLLCYYSPVLEKELEGTLFEPILTECPPEEVCYKAEGLYGNFSALLARGCMPRRECGQVDKVRVKGVIYTMHRTCCDSPHCNICTGITVTSALLFITVLEMSFCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQI5</accession>
    <name>A0A672ZQI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Extracellular calcium-sensing receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">casr</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Homodimer; disulfide-linked. Interacts with VCP. Interacts with ARRB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030010928.1">
        <property type="nucleotide sequence ID" value="XM_030155068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZQI5"/>
    <dbReference type="Ensembl" id="ENSSORT00005019202.1">
        <property type="protein sequence ID" value="ENSSORP00005018653.1"/>
        <property type="gene ID" value="ENSSORG00005009195.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433589"/>
    <dbReference type="CTD" id="846"/>
    <dbReference type="InParanoid" id="A0A672ZQI5"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005513">
        <property type="term" value="P:detection of calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051924">
        <property type="term" value="P:regulation of calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000388">
        <property type="entry name" value="Vomeronasal 2, receptor 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF358">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00592">
        <property type="entry name" value="CASENSINGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025677127" description="Extracellular calcium-sensing receptor" evidence="21">
        <location>
            <begin position="20"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="597"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="634"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="667"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="710"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="754"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="788"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="816"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="22">
        <location>
            <begin position="597"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="876"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="884"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="940" mass="105661" checksum="C5307E50686D0DF7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTC9</accession>
    <name>A0A672ZTC9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115429500</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 hydrogencarbonate(out) + Na(+)(out) = 2 hydrogencarbonate(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72215"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3 hydrogencarbonate(out) + Na(+)(out) = 3 hydrogencarbonate(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72219"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004862.1">
        <property type="nucleotide sequence ID" value="XM_030149002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZTC9"/>
    <dbReference type="Ensembl" id="ENSSORT00005020736.1">
        <property type="protein sequence ID" value="ENSSORP00005020169.1"/>
        <property type="gene ID" value="ENSSORG00005001202.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429500"/>
    <dbReference type="InParanoid" id="A0A672ZTC9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000002">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003024">
        <property type="entry name" value="Na/HCO3_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF10">
        <property type="entry name" value="ELECTROGENIC SODIUM BICARBONATE COTRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01232">
        <property type="entry name" value="NAHCO3TRSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="506"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="585"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="683"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="721"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="769"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="809"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="869"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="895"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="953"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="18">
        <location>
            <begin position="108"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="17">
        <location>
            <begin position="443"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="390"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1001"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1001"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1070" mass="120384" checksum="EE761EDA7B0C9644" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTN7</accession>
    <name>A0A672ZTN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">RING-type E3 ubiquitin-protein ligase PPIL2</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">CYC4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Probable inactive peptidyl-prolyl cis-trans isomerase-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ppil2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Has a ubiquitin-protein ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation. May also function as a chaperone, playing a role in transport to the cell membrane of BSG/Basigin for instance. Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the minor spliceosome, which splices U12-type introns. Within this complex, interacts with PRPF8/PRP8, EFTUD2/SNU114 and PLRG1. Interacts with isoform 2 of BSG. Interacts (via the PPIase cyclophilin-type domain) with CRNKL1; they may form a trimeric complex with HSP90.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_030000036.1">
        <property type="nucleotide sequence ID" value="XM_030144176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZTN7"/>
    <dbReference type="Ensembl" id="ENSSORT00005021257.1">
        <property type="protein sequence ID" value="ENSSORP00005020680.1"/>
        <property type="gene ID" value="ENSSORG00005009025.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426178"/>
    <dbReference type="CTD" id="23759"/>
    <dbReference type="InParanoid" id="A0A672ZTN7"/>
    <dbReference type="OrthoDB" id="30774at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01923">
        <property type="entry name" value="cyclophilin_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16663">
        <property type="entry name" value="RING-Ubox_PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000079">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000018">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026951">
        <property type="entry name" value="PPIL2_U-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN-PROTEIN LIGASE PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="23">
        <location>
            <begin position="35"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="22">
        <location>
            <begin position="285"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="449"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="492"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00073734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="527" mass="59424" checksum="68EBB08CCF10B03C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXR2</accession>
    <name>A0A672ZXR2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Interferon-induced protein with tetratricopeptide repeats 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115417640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the IFIT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZXR2"/>
    <dbReference type="FunCoup" id="A0A672ZXR2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021660.1">
        <property type="protein sequence ID" value="ENSSORP00005021033.1"/>
        <property type="gene ID" value="ENSSORG00005010266.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZXR2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000026">
        <property type="entry name" value="Interferon-induced protein with tetratricopeptide repeats 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271:SF14">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="468" mass="53877" checksum="0F90EC44B17898B9" modified="2020-06-17" version="1">SELIQSAAPPPPEHRLQVHLEALQCHFTWDLDSSRSRLLRLCDNLTDIGSEEGYVWLGHIYNLQGFIHHQLGDCDHALRFFSRAAEAFRQIRNTVSDQGPWLVVNYGNLAWLHHHLGEPAQSRRYLLKVDELMSEHPPPEQHDLHPDVYAEKAWTLMKFGRSPAAADLFQSAIRMQPDHVEWCSSRVLALVQAAPTLDDDIIDRMRIAKAHDPENLYLTALHLEALASRGQNVESEARELATRVLKKPASSYSGIRPLLRLYTMKISTSDATDLAEEALQRHPDHRYLKDCAAHCYNKRILLDRDQDQDRPNQSLVDRAISLFKDVIALYPDSSLKRKISLANIYAKTNTNLAEGQQIFDDLLRTDLDPEEAQMVYNYYAKFLHFYMNRSDESIRFHMAAAEIRPLTFYGEHSLRTLERIRDRNRNQRCPEILSSSELEQIRTFLDTFNCSFIFLINVALFGVVFSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXX6</accession>
    <name>A0A672ZXX6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TAR DNA-binding protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">tardbpb</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZXX6"/>
    <dbReference type="Ensembl" id="ENSSORT00005021970.1">
        <property type="protein sequence ID" value="ENSSORP00005021327.1"/>
        <property type="gene ID" value="ENSSORG00005010434.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZXX6"/>
    <dbReference type="OrthoDB" id="2020831at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19609">
        <property type="entry name" value="NTD_TDP-43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12321">
        <property type="entry name" value="RRM1_TDP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12322">
        <property type="entry name" value="RRM2_TDP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000098">
        <property type="entry name" value="TAR DNA-binding protein 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000107">
        <property type="entry name" value="TAR DNA-binding protein 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049124">
        <property type="entry name" value="TDP-43_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041105">
        <property type="entry name" value="TDP-43_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF9">
        <property type="entry name" value="TAR DNA-BINDING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20910">
        <property type="entry name" value="TDP-43_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18694">
        <property type="entry name" value="TDP-43_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="105"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="195"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="334"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="377"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="405" mass="43929" checksum="7B3144F7DD8DFE92" modified="2020-06-17" version="1">MAEVYIRVAEEENEEPMEIPSEDDGTVLLSTVAAQFPGACGLRFRSPVSQCMRGVRLVEGVLHAPENGWGNVVYVVNYPKDNKRKMDEIDASSAVKMKRGDMKTSDLIVLGLPWKTTEQDLKDYFSTFGEVIMVQVKRDAKTGNSKGFGFVRFTEYEAQEKVISQRHMIDGRWCDCKLPNSKVNMQGPDEPLRSRKVFVGRCTEDMTTDDLRQFFMQYGEVTDVFIPKPFRAFAFVTFADDQVAQSLCGEDLIIKGVSVHISNAEPKHGNRQFDRTARFGNGFGAQAFGSSRSGLGSSTNSSLANFGSFSLNPAMMAAAQAALQSSWGMMGMLASQQQTSTSGSTSSGTSSSRDQSQSFSTGNSNYGTSSASLGWGTGSNSTTSGSGFSSGFGSSMESKSSGWGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZYI3</accession>
    <name>A0A672ZYI3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIH1 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles. Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription. Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1. Positively regulates the assembly and activity of the mTORC1 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZYI3"/>
    <dbReference type="FunCoup" id="A0A672ZYI3">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022674.1">
        <property type="protein sequence ID" value="ENSSORP00005022019.1"/>
        <property type="gene ID" value="ENSSORG00005010754.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZYI3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF0">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="PIH1 N-terminal" evidence="5">
        <location>
            <begin position="33"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="6">
        <location>
            <begin position="207"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="278" mass="31786" checksum="B7BF49FEEDE7E5A1" modified="2020-06-17" version="1">RLDTFEALENEQKLLFVFLFFYLFLFFFHQTMGELQKENPDSRVIRPQPGLCVKTLSEPDKQKVFVNVCQSNAVPAPPQLSREELVELLQSDDPSGYRVPMSLGEPHTETDNSSQGCTAYDVVINQEFFQTCQKDPLFQQFIILVSVEGLENKYNLELSRDWKVLKNRKFLGSLSEQNIRTKSRPVIEELHTQYVPQFSLLVEPPAGQPQYLTAEIHLPGVSSCASLVLDVGEDRLVLTARPNLFHLDICHHLVIDQERSTAKPKRPPLTLKDPNVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0H8</accession>
    <name>A0A673A0H8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Syntaxin-11-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115415594</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029985081.1">
        <property type="nucleotide sequence ID" value="XM_030129221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A0H8"/>
    <dbReference type="Ensembl" id="ENSSORT00005023404.1">
        <property type="protein sequence ID" value="ENSSORP00005022739.1"/>
        <property type="gene ID" value="ENSSORG00005011044.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415594"/>
    <dbReference type="InParanoid" id="A0A673A0H8"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048787">
        <property type="term" value="C:presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF30">
        <property type="entry name" value="SYNTAXIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="213"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="300" mass="34611" checksum="27B134B22E18D91A" modified="2020-06-17" version="1">MRDMLERLQTISEEHEDLESEFYGPECDIDKITLTQEAVVFENSSAIDNILKEAYAIRKEISLLHLEVERLGVHNERFRTSMRRFTLLKKDSDSIARGIHQRGEALYRCLQALGNESRRLEQSEGPHSAVSRVAKVQYDTLILAFHDTMSDYNKAEELQRSTCRGRIQRQASIIGTEITDEQLDVLVDKGGEGWAELSQSLQTTGGRTSRYALCEIKGRHKELVELEARLKEVHELFLHMAVLVEDQGFVMNNIQANIRSTQEYVEKINVDIKRALQYKRKNPFGQCCPCLPCWKFNQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1D7</accession>
    <name>A0A673A1D7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Adenosine deaminase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mapda</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-methyl-AMP + H2O + H(+) = IMP + methylamine</text>
            <dbReference type="Rhea" id="RHEA:16001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:59338"/>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16002"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A1D7"/>
    <dbReference type="FunCoup" id="A0A673A1D7">
        <property type="interactions" value="562"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023965.1">
        <property type="protein sequence ID" value="ENSSORP00005023290.1"/>
        <property type="gene ID" value="ENSSORG00005011276.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A1D7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00443">
        <property type="entry name" value="ADA_AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000033">
        <property type="entry name" value="Adenosine deaminase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF42">
        <property type="entry name" value="ADENOSINE DEAMINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="39731" checksum="DFD2458CBA9E8D15" modified="2020-06-17" version="1">ALCKLVMDNEVDVFYRELPKVELHAHLNGSVSFQTIEKLISRKPHLNIEHGMTAIGKGQRRTLDECFQVFKVIHQLVDTEEDILMVATDVIKEFAADGVKYLELRSTPREEKNTGLTKKRYIETVMKAIQQCRKEGVDIDVRFLVAIDRRNGTEVAMETVKLAEEFMLSSDGLVVGLDLSGDPTVGHGRDLLPALQKAKNCGLKLSLHLSEVPSQLEESDLLLNLPPDRIGHGTFLHPEVGGSASLVEKVVKNNIPLELCLTSNIKGQTVPCYAQHHFKYWYQMGHPSVICTDDKGVFCTDLSQEYQVAASTFGLSREAVWKLSQSAIDCIFAPDSLKQQLKQKWMDLQPQVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2F5</accession>
    <name>A0A673A2F5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane anterior posterior transformation 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tapt1b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A2F5"/>
    <dbReference type="FunCoup" id="A0A673A2F5">
        <property type="interactions" value="651"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024090.1">
        <property type="protein sequence ID" value="ENSSORP00005023414.1"/>
        <property type="gene ID" value="ENSSORG00005010371.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A2F5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045724">
        <property type="term" value="P:positive regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="488"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="556" mass="62979" checksum="4A241F9BD69B563F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2V7</accession>
    <name>A0A673A2V7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cmc2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CMC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029983869.1">
        <property type="nucleotide sequence ID" value="XM_030128009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A2V7"/>
    <dbReference type="FunCoup" id="A0A673A2V7">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023629.1">
        <property type="protein sequence ID" value="ENSSORP00005022958.1"/>
        <property type="gene ID" value="ENSSORG00005011122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414717"/>
    <dbReference type="CTD" id="56942"/>
    <dbReference type="InParanoid" id="A0A673A2V7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22977">
        <property type="entry name" value="COX ASSEMBLY MITOCHONDRIAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22977:SF1">
        <property type="entry name" value="COX ASSEMBLY MITOCHONDRIAL PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="78" mass="9577" checksum="BFFB11D0F0E2056B" modified="2020-06-17" version="1">MHTDLSPHLHTDECNELISRLKLCHKEHNFLKFFGTCNDFDREVRHCLKKERLEKRERSKQRALEMRRKLKEGPKEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A848</accession>
    <name>A0A673A848_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Nicotinamide phosphoribosyltransferase</fullName>
            <shortName evidence="7">NAmPRTase</shortName>
            <ecNumber evidence="4 7">2.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nampt2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>beta-nicotinamide D-ribonucleotide + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16149"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.12"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:16151"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.12" evidence="4 7"/>
    <dbReference type="AlphaFoldDB" id="A0A673A848"/>
    <dbReference type="FunCoup" id="A0A673A848">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025494.1">
        <property type="protein sequence ID" value="ENSSORP00005024762.1"/>
        <property type="gene ID" value="ENSSORG00005011610.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A848"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00890"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047280">
        <property type="term" value="F:nicotinamide phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01569">
        <property type="entry name" value="PBEF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041529">
        <property type="entry name" value="DUF5598"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016471">
        <property type="entry name" value="Nicotinamide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006629">
        <property type="entry name" value="PRK09198.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43816">
        <property type="entry name" value="NICOTINAMIDE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43816:SF2">
        <property type="entry name" value="NICOTINAMIDE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18127">
        <property type="entry name" value="NAMPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005943">
        <property type="entry name" value="NMPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="7" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 7" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinamide phosphoribosyltransferase N-terminal" evidence="9">
        <location>
            <begin position="32"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="8">
        <location>
            <begin position="196"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="509" mass="57824" checksum="60224FCC1484DDE4" modified="2020-06-17" version="1">NCGKNGDINSSRCIHVKVNVCCSCLINLCFFFQITHYKQYPPNVSKIYSYFECRRKKGSQFSEVVFFGLQYLLKKYLTGPVITEEKIQEAKLFYQMHFKQAVFDEEGWRKVLEKHDGRLPIRIKAVPEGRIIPRGNVLFTVENTDPSFYWLTNYIETMLVQMWYPITVATVSREFKKILARHLKATSGSLEGLDLKLHDFGYRGVSSQEVRGTSSAHLVNFCSTDTVAGLLMAQRYYSCPMAGFSIPAAEHSTIISWGRTREKEAFERVLDQFSSGPVSVVSDSYDIFNACKHIWGDKLKERVMERSQDSCLVIRPDSGDPAETLIEVIKILEECFGCSLNAMGFKVLPSYLRIIQGDGIDLTSVDQILQKLSDEGWSAENIFFGCGSALLQKLNRDTLSCAFKCSYVETNGKGMDVYKQPVTDPSKGSKRGRLSLRRNSDGFIETVERGAGKPEEDLLVTVFENGSLMHEYSLEEIRKNARLRDDEMDHTPHNQEAELLHNHIINGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8I8</accession>
    <name>A0A673A8I8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A8I8"/>
    <dbReference type="Ensembl" id="ENSSORT00005025752.1">
        <property type="protein sequence ID" value="ENSSORP00005025003.1"/>
        <property type="gene ID" value="ENSSORG00005012054.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A8I8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="158" mass="18622" checksum="525F2AA8C790CFCB" modified="2020-06-17" version="1">MFLCEIFLHLFHSFVEVEEGKEFVVLPFITTPESLDGVEVRWFYYEDDECKELLVYPKGSVEHHDDYRNRTEMNENPLGSGNLSLTLSDPKDTDSGKYRCYVKKNNNIITMKTVLLKVHHLWFILCLWLVVYLMVNLKINHTTVVSLVPYILLCVFHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A9S5</accession>
    <name>A0A673A9S5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 13 member 5b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">slc13a5b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A9S5"/>
    <dbReference type="FunCoup" id="A0A673A9S5">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026727.1">
        <property type="protein sequence ID" value="ENSSORP00005025959.1"/>
        <property type="gene ID" value="ENSSORG00005012108.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A9S5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015137">
        <property type="term" value="F:citrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017153">
        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015741">
        <property type="term" value="P:fumarate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015729">
        <property type="term" value="P:oxaloacetate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031312">
        <property type="entry name" value="Na/sul_symport_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF134">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01271">
        <property type="entry name" value="NA_SULFATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="458"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="581" mass="64350" checksum="A55F8FB459113759" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAD3</accession>
    <name>A0A673AAD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Beta-galactosidase</fullName>
            <ecNumber evidence="5 16">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">glb1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. May form higher multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="5 16"/>
    <dbReference type="RefSeq" id="XP_029995842.1">
        <property type="nucleotide sequence ID" value="XM_030139982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AAD3"/>
    <dbReference type="Ensembl" id="ENSSORT00005027003.1">
        <property type="protein sequence ID" value="ENSSORP00005026231.1"/>
        <property type="gene ID" value="ENSSORG00005009686.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423208"/>
    <dbReference type="CTD" id="2720"/>
    <dbReference type="InParanoid" id="A0A673AAD3"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042340">
        <property type="term" value="P:keratan sulfate proteoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000021">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000115">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000017">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026283">
        <property type="entry name" value="B-gal_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048912">
        <property type="entry name" value="BetaGal1-like_ABD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21317">
        <property type="entry name" value="BetaGal_ABD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006336">
        <property type="entry name" value="B-gal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025348689" description="Beta-galactosidase" evidence="18">
        <location>
            <begin position="23"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="19">
        <location>
            <begin position="36"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase 1-like first all-beta" evidence="20">
        <location>
            <begin position="398"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase galactose-binding" evidence="21">
        <location>
            <begin position="537"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="649" mass="72025" checksum="3C1D8BC01BD268BA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAF1</accession>
    <name>A0A673AAF1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear receptor subfamily 2 group F member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">nr2f5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AAF1"/>
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        <property type="protein sequence ID" value="ENSSORP00005025693.1"/>
        <property type="gene ID" value="ENSSORG00005012353.1"/>
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    <dbReference type="OrthoDB" id="5873264at2759"/>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06958">
        <property type="entry name" value="NR_DBD_COUP_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NR_LBD_COUP-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000003">
        <property type="entry name" value="Coup transcription factor 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor subfamily 2 group F member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
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    </dbReference>
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        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="51"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="152"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
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            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
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        <location>
            <begin position="127"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="404" mass="45028" checksum="CB4A3AF128495A85" modified="2020-06-17" version="1">MAMVVNQWPETISADPGSQLQICGQEPGGGPGTPNGSTPGNDALSGDKIPNVDCMVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLTYTCRGNRDCPIDQHHRNQCQYCRLKKCLKVGMRREAVQRGRTSNSQSSPGQYLTNGTDPYNGQPYLSGFISLLLRAEPYPTSRYGAQCMQGNNLMGIENICELAARLLFSAVEWAKNIPFFPDLQLMDQVALLRMSWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSAERVVAFMDHIRVFQEQVEKLKALQVDTAEYSCLKSIVLFTSDAMGLSDVAHVESIQEKSQCALEEYVRNQYPSQPNRFGRLLLRLPSLRIVSSPVIEQLFFVRLVGKTPIETLLRDMLLSGSSYNWPYMPTVQRDRPLSLHYNENGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACP3</accession>
    <name>A0A673ACP3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:dkey-16i5.8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115439702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ACP3"/>
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        <property type="protein sequence ID" value="ENSSORP00005026277.1"/>
        <property type="gene ID" value="ENSSORG00005012606.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027059.1">
        <property type="protein sequence ID" value="ENSSORP00005026286.1"/>
        <property type="gene ID" value="ENSSORG00005012613.1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TMEM238"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613">
        <property type="entry name" value="SI:CH211-232M10.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613:SF9">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15125">
        <property type="entry name" value="TMEM238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="152" mass="16840" checksum="2D8C349B09D3B910" modified="2020-06-17" version="1">MSSTTTMSTSVQVEPVMERAYGGVGRCKCSFWFAVAHDILGVFIMMVGVFGGLVIHDLFIYAGAIVIFLSLIWWVFWYSGNIDVPPEELEDDVGMMKLKNRGLSKVVRQVSDRISSGIRDSFRKSSRSNTRRSTSGHTANGSGLENTALCYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AD53</accession>
    <name>A0A673AD53_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell death inducing DFFA like effector c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cidec</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AD53"/>
    <dbReference type="FunCoup" id="A0A673AD53">
        <property type="interactions" value="1306"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027996.1">
        <property type="protein sequence ID" value="ENSSORP00005027211.1"/>
        <property type="gene ID" value="ENSSORG00005013001.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AD53"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003508">
        <property type="entry name" value="CIDE-N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12306">
        <property type="entry name" value="CELL DEATH ACTIVATOR CIDE"/>
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        <property type="entry name" value="LIPID TRANSFERASE CIDEC"/>
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        <property type="entry name" value="CIDE-N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CAD"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="248" mass="27785" checksum="D47AD0B42D7A0288" modified="2020-06-17" version="1">MDYAMKSLGLLAPSSLSKCVTAASSSMTQQLLSARPPRQKPFRVTNADRSVKKGIMAHTVEDLMNKVSNSLNVPCVSALVLDEDGTGVDTEDFFQTLPENAVLMVLEKGQKWTPHLVRQQYVVRNSSTTDQLSQCRPKHRTDVARVTFDLYKNNPKDFIGCLNVKATLYGAYSVSYDLRCYAAKKMLKEALRWAIFSMQATGHILLGSSCYIEQLLEEEERAENGLTLPQESRIKQLQGMLLGKVFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADU0</accession>
    <name>A0A673ADU0_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Transmembrane protein 185A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115438138</name>
        <name evidence="3" type="synonym">LOC115438137</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSSORP00005027466.1"/>
        <property type="gene ID" value="ENSSORG00005013120.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028277.1">
        <property type="protein sequence ID" value="ENSSORP00005027488.1"/>
        <property type="gene ID" value="ENSSORG00005013136.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="entry name" value="TM_Fragile-X-F-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568">
        <property type="entry name" value="FAM11A, B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13568:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10269">
        <property type="entry name" value="Tmemb_185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="352" mass="40555" checksum="F2930B9E826BEFD5" modified="2020-06-17" version="1">MIVQDFPFSSTHGKFLIYSCLLLFSVLLSLRLDGVIQWSYWAVFTPIWLWKLLVIIGASVGTGVWAHNPQYRAEGETCVEFKAMLIAVGLHVLLLMFEVLVCDRVARGNYFWLLVFMPLFFVSPVSVAACVWGFRHDRSLELEVLCSVNILQFIFIALRLDRIINWPWLVVCVPLWILMSFLCLVVLYYIIWSVLFLRSIDIIAEQRRTHITMAISWMTIVVPLLTFEILLVHKLDGHNSLSYVCVFVPLWLSLLTLMATTFGQKGGNHWWFGIRKDFCHFLLELLPFLREYGNVSYDLQRSEDPEAAEDLPVPEPPPKIAPMFHKKTGVVITQSPGKYFVPPPKLCIDMPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AG89</accession>
    <name>A0A673AG89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zyxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">zyx</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the zyxin/ajuba family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030014125.1">
        <property type="nucleotide sequence ID" value="XM_030158265.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030014126.1">
        <property type="nucleotide sequence ID" value="XM_030158266.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030014127.1">
        <property type="nucleotide sequence ID" value="XM_030158267.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030014128.1">
        <property type="nucleotide sequence ID" value="XM_030158268.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030014129.1">
        <property type="nucleotide sequence ID" value="XM_030158269.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030014130.1">
        <property type="nucleotide sequence ID" value="XM_030158270.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030014131.1">
        <property type="nucleotide sequence ID" value="XM_030158271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AG89"/>
    <dbReference type="FunCoup" id="A0A673AG89">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029105.1">
        <property type="protein sequence ID" value="ENSSORP00005028296.1"/>
        <property type="gene ID" value="ENSSORG00005013543.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435687"/>
    <dbReference type="CTD" id="7791"/>
    <dbReference type="InParanoid" id="A0A673AG89"/>
    <dbReference type="OrthoDB" id="25414at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09435">
        <property type="entry name" value="LIM3_Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000027">
        <property type="entry name" value="lipoma-preferred partner isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000057">
        <property type="entry name" value="Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212:SF1">
        <property type="entry name" value="ZYXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212">
        <property type="entry name" value="ZYXIN/TRIP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="433"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="495"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="553"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="626" mass="66365" checksum="834ACB1962814719" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGM9</accession>
    <name>A0A673AGM9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted frizzled-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">sfrp5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secreted frizzled-related protein (sFRP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030012609.1">
        <property type="nucleotide sequence ID" value="XM_030156749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AGM9"/>
    <dbReference type="Ensembl" id="ENSSORT00005029208.1">
        <property type="protein sequence ID" value="ENSSORP00005028399.1"/>
        <property type="gene ID" value="ENSSORG00005013586.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434675"/>
    <dbReference type="CTD" id="6425"/>
    <dbReference type="InParanoid" id="A0A673AGM9"/>
    <dbReference type="OrthoDB" id="5985572at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07444">
        <property type="entry name" value="CRD_SFRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03580">
        <property type="entry name" value="NTR_Sfrp1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000003">
        <property type="entry name" value="Secreted frizzled-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000001">
        <property type="entry name" value="secreted frizzled-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041761">
        <property type="entry name" value="SFRP5_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF46">
        <property type="entry name" value="SECRETED FRIZZLED-RELATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025393410" description="Secreted frizzled-related protein 1" evidence="12">
        <location>
            <begin position="38"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="13">
        <location>
            <begin position="58"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="14">
        <location>
            <begin position="191"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="73"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="321" mass="36688" checksum="CC842AD7869F068F" modified="2020-06-17" version="1">MAHGQKSPRWALTQGSFSLALGLSLFSLLLLLLTVSAADEYDYYSWQSDNFHNGRFYTKQPQCVDIPADLRLCHNVGYKKMRLPNLLDHETMPEVKQQAGSWVPLLAKRCHADTQVFLCSLFAPVCLDRPIYPCRSLCEAVRDSCAPVMETYGFPWPEMLTCDKFPIDNDLCIPMQFTGNHATQPPVSKVCPPCDNELKTDNIMEHYCASDFALKMKIKEVKKEKGDRKLIAAQKKKKVLKQGVLRKKDLKKLTLYIKNGANCPCSQLDSLGSNFLIMGRKVDQQLLLMSIHKWDKKSKELKFAIKYMKSHQCPTYHTVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHM5</accession>
    <name>A0A673AHM5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Si:ch211-13f8.2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AHM5"/>
    <dbReference type="Ensembl" id="ENSSORT00005028639.1">
        <property type="protein sequence ID" value="ENSSORP00005027842.1"/>
        <property type="gene ID" value="ENSSORG00005013305.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AHM5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050211">
        <property type="term" value="F:procollagen galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06532">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000048">
        <property type="entry name" value="Glycosyltransferase 25 family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF8">
        <property type="entry name" value="PROCOLLAGEN GALACTOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13704">
        <property type="entry name" value="Glyco_tranf_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl transferase family 25" evidence="6">
        <location>
            <begin position="322"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="563"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="596"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="610" mass="70478" checksum="3484D50C42EE5CF7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AK76</accession>
    <name>A0A673AK76_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone H1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AK76"/>
    <dbReference type="FunCoup" id="A0A673AK76">
        <property type="interactions" value="1306"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030487.1">
        <property type="protein sequence ID" value="ENSSORP00005029654.1"/>
        <property type="gene ID" value="ENSSORG00005014154.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AK76"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000075">
        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="6">
        <location>
            <begin position="53"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="132"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="225" mass="23611" checksum="5B9583D5950AF72C" modified="2020-06-17" version="1">TQVRYRFNQCPPSEQVLDLFIRLLSVKRKHKSPAPAAAPAKAAKKKVVKKAKTGPSVRDLIIKTVAASKERNGVSLAALKKALAAAGYDVEKNKGRIKVAVKSLVASGVLTQTKGTGASGSFKMSKKAETKPKKKAVKKVASKAKKAAAKKPATPKRPKKPAAAKKVPKKSPKKAAKKAPAAKKVTAKSPKKAPKKATKKPAAKKAPAKKAAAKPKAKKAAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALM8</accession>
    <name>A0A673ALM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="9">MAD homolog</shortName>
            <shortName evidence="9">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115425515</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029999003.1">
        <property type="nucleotide sequence ID" value="XM_030143143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ALM8"/>
    <dbReference type="FunCoup" id="A0A673ALM8">
        <property type="interactions" value="2982"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030391.1">
        <property type="protein sequence ID" value="ENSSORP00005029558.1"/>
        <property type="gene ID" value="ENSSORG00005013998.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425515"/>
    <dbReference type="InParanoid" id="A0A673ALM8"/>
    <dbReference type="OrthoDB" id="5794312at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10491">
        <property type="entry name" value="MH1_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10985">
        <property type="entry name" value="MH2_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF42">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="11">
        <location>
            <begin position="10"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="12">
        <location>
            <begin position="274"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="222"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="467" mass="52341" checksum="BE3FA987D773AB7E" modified="2020-06-17" version="1">MSSILPFTPPVVKRLLGWKKSTSGPGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNCNTKCVTIPSNCSEIWGLSTPNTIEQWDTSGLYSYPDQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAIEACEYAFHLKKDEVCINPYHYQRVETPVLPPVLVPRHSEILTELPPLDDYTHSIPENTNFPAGIEPPNNYIPETPPPGYISEDGEASDQQMNQSMDTGSPAELSPSTLSPVNHSMDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSARCSSMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANJ7</accession>
    <name>A0A673ANJ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Catalase</fullName>
            <ecNumber evidence="15">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cat</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A673ANJ7"/>
    <dbReference type="FunCoup" id="A0A673ANJ7">
        <property type="interactions" value="1325"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031811.1">
        <property type="protein sequence ID" value="ENSSORP00005030946.1"/>
        <property type="gene ID" value="ENSSORG00005014761.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ANJ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08156">
        <property type="entry name" value="catalase_clade_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000001">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040333">
        <property type="entry name" value="Catalase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 15" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 15" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="18">
        <location>
            <begin position="28"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="543" mass="62198" checksum="7D887E1D12BD872C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APG4</accession>
    <name>A0A673APG4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G protein-coupled receptor 61-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673APG4"/>
    <dbReference type="Ensembl" id="ENSSORT00005032445.1">
        <property type="protein sequence ID" value="ENSSORP00005031565.1"/>
        <property type="gene ID" value="ENSSORG00005015019.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APG4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15220">
        <property type="entry name" value="7tmA_GPR61_GPR62-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF11">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="52"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="396"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="47416" checksum="2D88AEC3A37DEA22" modified="2020-06-17" version="1">MAGTSGPMVAPVPNLTTALWELNPTVPAEVGVVTSSQSQIKDLFGLFCMVTLNLIALLANTGVMVAIARAPHLKRFAFVCHLCSVDLLCAILLMPLGIISSSPFFGTVVFTVLECQVYIFLNVFLICLSILTITAISVERYFYIVHPMRYEVKMTINLAIGVMLLIWVKSLALALVTLFGWPAYGNHSSIAAAHCSLHASHSRLRRVFAVLFSMVCFLVPAVVIFTVYCAVYKVARSAALQQVPAVPTWTNTSPAKNRSDSINSQTTMINTSRTLPQRLSPERAFSGGKAALTLIFIVGQFLICWLPYFIFHLQMSLTDSMQSPGDLEEAVTWLAYSSFAVNPFFYGLLNRQIREELIKFRRCCLTQPLELGASSHEGSLQENFLQFIQRTTSTTETRSSCATSSPRNTLDQATDYTRPLSSDGDSLTCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APK6</accession>
    <name>A0A673APK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynamin-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Scaffold protein Tuba</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">arhgef38</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029989369.1">
        <property type="nucleotide sequence ID" value="XM_030133509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673APK6"/>
    <dbReference type="Ensembl" id="ENSSORT00005031348.1">
        <property type="protein sequence ID" value="ENSSORP00005030493.1"/>
        <property type="gene ID" value="ENSSORG00005014540.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418920"/>
    <dbReference type="CTD" id="54848"/>
    <dbReference type="InParanoid" id="A0A673APK6"/>
    <dbReference type="OrthoDB" id="6244550at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12141">
        <property type="entry name" value="SH3_DNMBP_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000066">
        <property type="entry name" value="dynamin-binding protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000023">
        <property type="entry name" value="dynamin-binding protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000061">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 38"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
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    <dbReference type="InterPro" id="IPR051492">
        <property type="entry name" value="Dynamin-Rho_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834">
        <property type="entry name" value="NUCLEAR FUSION PROTEIN FUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834:SF17">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="100"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="BAR" evidence="12">
        <location>
            <begin position="331"/>
            <end position="557"/>
        </location>
    </feature>
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        <location>
            <begin position="604"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="742"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="721"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="460"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="731"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="806" mass="92471" checksum="FDAB780B7E463008" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQR3</accession>
    <name>A0A673AQR3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcium/calmodulin-dependent protein kinase II inhibitor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">camk2n1a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the CAMK2N family.</text>
    </comment>
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        <property type="term" value="F:calcium-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="CaM-KIIN"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00009996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8529" checksum="ECAD2A2D8D6E2595" modified="2020-06-17" version="1">MSEVLPYNEGKMSGYGADSEVSQMSFSCGLQDTNAFFAASQAKRPPKLGQIGRAKRVVIEDDRIDEVLKGMTDKSSPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATL9</accession>
    <name>A0A673ATL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">carnitine O-palmitoyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115410801</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:12662"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673ATL9"/>
    <dbReference type="Ensembl" id="ENSSORT00005033914.1">
        <property type="protein sequence ID" value="ENSSORP00005033010.1"/>
        <property type="gene ID" value="ENSSORG00005014883.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATL9"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015909">
        <property type="term" value="P:long-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000002">
        <property type="entry name" value="carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF114">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="20">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="19">
        <location>
            <begin position="171"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="758"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="763"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="790" mass="90096" checksum="4EB9798BA1795C18" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYH1</accession>
    <name>A0A673AYH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Optineurin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">optn</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">May act by regulating membrane trafficking and cellular morphogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
            <location evidence="13">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus</location>
            <location evidence="2 13">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030006150.1">
        <property type="nucleotide sequence ID" value="XM_030150290.1"/>
    </dbReference>
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        <property type="interactions" value="309"/>
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    <dbReference type="Ensembl" id="ENSSORT00005035785.1">
        <property type="protein sequence ID" value="ENSSORP00005034861.1"/>
        <property type="gene ID" value="ENSSORG00005016426.1"/>
    </dbReference>
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    <dbReference type="CTD" id="10133"/>
    <dbReference type="InParanoid" id="A0A673AYH1"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:autophagosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090161">
        <property type="term" value="P:Golgi ribbon formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09803">
        <property type="entry name" value="UBAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.390:FF:000002">
        <property type="entry name" value="NF-kappa-B essential modulator isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.390">
        <property type="entry name" value="L1 transposable element, trimerization domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.990">
        <property type="entry name" value="Nemo cc2-lz domain - 1d5 darpin complex"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CC2-LZ_dom"/>
    </dbReference>
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        <property type="entry name" value="NEMO_N"/>
    </dbReference>
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        <property type="entry name" value="NEMO_ZF"/>
    </dbReference>
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        <property type="entry name" value="Optineurin/NFkB_EssMod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31553">
        <property type="entry name" value="NF-KAPPA-B ESSENTIAL MODULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31553:SF2">
        <property type="entry name" value="OPTINEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16516">
        <property type="entry name" value="CC2-LZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11577">
        <property type="entry name" value="NEMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18414">
        <property type="entry name" value="zf_C2H2_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51801">
        <property type="entry name" value="ZF_CCHC_NOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 13" id="KW-0967">Endosome</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC NOA-type" evidence="16">
        <location>
            <begin position="502"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="22"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="532" mass="60381" checksum="9D39A8EC5A53EBF0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0R5</accession>
    <name>A0A673B0R5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aminoadipate-semialdehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aasdh</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035044.1">
        <property type="protein sequence ID" value="ENSSORP00005034128.1"/>
        <property type="gene ID" value="ENSSORG00005016140.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B0R5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052091">
        <property type="entry name" value="Beta-ala_Activ/Resist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394:SF1">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13570">
        <property type="entry name" value="Beta-prop_ACSF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="4">
        <location>
            <begin position="405"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="488"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="911" mass="99336" checksum="CCAFE525EDDBB494" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2E0</accession>
    <name>A0A673B2E0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gem</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Binds GTP saturably and exhibits a low intrinsic rate of GTP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with Calmodulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the small GTPase superfamily. RGK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B2E0"/>
    <dbReference type="FunCoup" id="A0A673B2E0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036942.1">
        <property type="protein sequence ID" value="ENSSORP00005035985.1"/>
        <property type="gene ID" value="ENSSORG00005016918.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2E0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04148">
        <property type="entry name" value="RGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001032">
        <property type="entry name" value="GTP-binding protein REM 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017358">
        <property type="entry name" value="RGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051641">
        <property type="entry name" value="RGK_GTP-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775:SF4">
        <property type="entry name" value="GTP-BINDING PROTEIN GEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775">
        <property type="entry name" value="RAD, GEM/KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038017">
        <property type="entry name" value="GTP-binding_GEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="310" mass="34369" checksum="7E574D3356E16F5F" modified="2020-06-17" version="1">MLSSVRRHSLRLQSELHRWSICDPGSHLLPDGLLARVPACISRSKSCTSSAGESDSSRGSWSSSDSVISSDSAGDQTGPGNPYRVVLLGASGVGKTAFASIFAGAADIQSDFTYEKCFISDEVCEKTVEVDGEPATITLLDTWDAETDTDCQSSQQCNMQTGDAYLLLYSITDRPSFLRASELRITLRQFRPVHHTPIILVGNKCDLVRRREVSVSEGRACAAVFDCKFIETSAAMQHNVWEAFHGIVRQLRLRRDSKEANKRRRYISSNSRRESLPVKAKRFLDKMVAKNNPSVAFWLKSKSCHDLSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4I2</accession>
    <name>A0A673B4I2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small EDRK-rich factor-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">serf2b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SERF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B4I2"/>
    <dbReference type="FunCoup" id="A0A673B4I2">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036358.1">
        <property type="protein sequence ID" value="ENSSORP00005035408.1"/>
        <property type="gene ID" value="ENSSORG00005016719.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B4I2"/>
    <dbReference type="OrthoDB" id="18018at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040211">
        <property type="entry name" value="SERF1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13596:SF0">
        <property type="entry name" value="SI:CH211-39K3.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13596">
        <property type="entry name" value="SMALL EDRK-RICH FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="62" mass="6867" checksum="39EBAAD837C70F50" modified="2020-06-17" version="1">MTRGNQRELARQKNAKKQNEQGKGKRGDDGLSAAARKQRDAEIMQQKQKKADEGGKPGSKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4M1</accession>
    <name>A0A673B4M1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DnaJ homolog subfamily B member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dnajb1b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSSORP00005035632.1"/>
        <property type="gene ID" value="ENSSORG00005016822.1"/>
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        <property type="term" value="F:transcription corepressor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="term" value="P:protein folding"/>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 4"/>
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        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="dnaJ homolog subfamily B member 5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DnaJ_C"/>
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        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
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        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF568">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="39350" checksum="DC2DF9555ADD94BE" modified="2020-06-17" version="1">MVKMGKDYYDILGIKKGASEDEIKKAYRKQALRYHPDKNKSPGAEEKFKEIAEAYDVLSDPKKKDIYDRFGEEGLKGGGPTGGCNGGPGTYSYTFQGDPHAIFAEFFGGRNPFEQFFGGRNGGMDENMDTNDPFAHFGMGGAGMGGGGMGGGGMGGFPRSFSTGMGGMGPHSSVVKKQQDPAVVRDLRVTLEEVLSGCTKKMKVSRKRLNPDGRTVRSEDKILEVQIKKGWKEGTKITFPKEGDETPTNIPADVVFVLKDKPHQVFKRDGSDIIYTAKISLRDALCGCTINAPTLEGRTVTVTTTDIVQPGMKRRVSGEGLPYPKRPDRRGDLIVEYEVKFPERLSQSAKDTIAQVLPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B532</accession>
    <name>A0A673B532_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115423252</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAMHD1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B532"/>
    <dbReference type="Ensembl" id="ENSSORT00005037888.1">
        <property type="protein sequence ID" value="ENSSORP00005036920.1"/>
        <property type="gene ID" value="ENSSORG00005016891.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B532"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="3">
        <location>
            <begin position="95"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="531" mass="62781" checksum="4D0B96CD7F25D1F4" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B8K6</accession>
    <name>A0A673B8K6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Carboxypeptidase O</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B8K6"/>
    <dbReference type="Ensembl" id="ENSSORT00005039671.1">
        <property type="protein sequence ID" value="ENSSORP00005038676.1"/>
        <property type="gene ID" value="ENSSORG00005018071.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B8K6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000084">
        <property type="entry name" value="Carboxypeptidase B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF19">
        <property type="entry name" value="CARBOXYPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="11">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="31642" checksum="1653B05D4824813A" modified="2020-06-17" version="1">FQHQTQQLDVVTKVNYGKTYENRTISLLWHPIGCKSASAKKAIWMDCRGWISPAFCEYFINQLLQTYKTDPEMEQMMKNMDFSVTPVLNLNLLTFRKPSKISTVINIGLSLLYYCAVAAVGVSFDWCSESYPGKSTMSRDKLMKSCFLTIHSYGQKLLIPYGHSNAIIPNENELMEVVGTSPDVLFKYTANLNSGSSRDWARLQGIPFAFTFELRDDGTYSFELPEDQIQPACEEAYSGALHIIIHVQDKEFIKPTGTAATVTATLWTTMMAVFVTSTAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAW4</accession>
    <name>A0A673BAW4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BAW4"/>
    <dbReference type="FunCoup" id="A0A673BAW4">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038752.1">
        <property type="protein sequence ID" value="ENSSORP00005037767.1"/>
        <property type="gene ID" value="ENSSORG00005017725.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BAW4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="156" mass="17311" checksum="FBB92C577E4EBEC6" modified="2020-06-17" version="1">MDSLMSLSSPLKQFTSCMSGTNKPTNKRGSKRSQSVRRSSFSRRMSIGRRRSLPCSSQKVPDSWLRVYQDELKRERKRQQANLAKKNAERTVRRTQFRSHHCLPRQTTAARKPAAVKDESLFGAFQGLSLDGLMGGANGSPAVSTSAAGGDQCKMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCB8</accession>
    <name>A0A673BCB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Core histone macro-H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115434488</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Variant histone H2A which replaces conventional H2A in a subset of nucleosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030012321.1">
        <property type="nucleotide sequence ID" value="XM_030156461.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030012322.1">
        <property type="nucleotide sequence ID" value="XM_030156462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BCB8"/>
    <dbReference type="FunCoup" id="A0A673BCB8">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040434.1">
        <property type="protein sequence ID" value="ENSSORP00005039425.1"/>
        <property type="gene ID" value="ENSSORG00005018394.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434488"/>
    <dbReference type="CTD" id="55506"/>
    <dbReference type="InParanoid" id="A0A673BCB8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02904">
        <property type="entry name" value="Macro_H2A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000013">
        <property type="entry name" value="Core histone macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.220.10:FF:000002">
        <property type="entry name" value="Core histone macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021171">
        <property type="entry name" value="Core_histone_macro-H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035796">
        <property type="entry name" value="Macro_H2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037942">
        <property type="entry name" value="Core_histone_macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7 8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Macro" evidence="11">
        <location>
            <begin position="181"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate" evidence="9">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="9">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037942-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="369" mass="40010" checksum="FB4D084E4E08AD4F" modified="2020-06-17" version="1">MSARGAKKKATKLSRSSRAGVIFPVGRMMRYLRTGTHKYRIGMGAPVYMAAVIEYLAAEILELAGNAARDNKKSRITPRHIKLAVANDEELNQLLRGVTISNGGVLPRIHPELLSKKRGSRVKVDSQATVADKQEEASKKKRSKPVKKVKGKRGRKPKSTDADKESVSNSNMEDGPGDGFTILSAKSLFLGQKLSLTESELSKLGSIKVEGIINPTNADMDLKDGVGNALEKAGGREFLDGVKELRKAQGPLEVASVAVSQANGMAARFVIHCNIPQWGSDKCEDQLEKTVKNCLSAAEEKKLKSVAFPSLPAGRNGFPKQTAAQLILKAISNHFVSSTSSSLKNIYFVLFDSESIGIYLQEMAKLDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCM5</accession>
    <name>A0A673BCM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.86</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">inppl1a</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25528"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="EC" id="3.1.3.86"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:25529"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.86" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673BCM5"/>
    <dbReference type="FunCoup" id="A0A673BCM5">
        <property type="interactions" value="983"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039174.1">
        <property type="protein sequence ID" value="ENSSORP00005038183.1"/>
        <property type="gene ID" value="ENSSORG00005017858.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BCM5"/>
    <dbReference type="Proteomes" id="UP000472271">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043569">
        <property type="term" value="P:negative regulation of insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09101">
        <property type="entry name" value="INPP5c_SHIP2-INPPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10343">
        <property type="entry name" value="SH2_SHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000005">
        <property type="entry name" value="phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_SHIP1-2_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057510">
        <property type="entry name" value="C2_SHIP1-2_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
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    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
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        <property type="entry name" value="SH2"/>
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    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 5-PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24147">
        <property type="entry name" value="C2_SHIP1-2_2nd"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2_SHIP1-2_first"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH2"/>
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        <property type="entry name" value="SH2DOMAIN"/>
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        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SAM_DOMAIN"/>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 20" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="22">
        <location>
            <begin position="24"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="23">
        <location>
            <begin position="1196"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="124"/>
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    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="887"/>
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    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1090"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="124"/>
            <end position="134"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="147"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="919"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="1041"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="1096"/>
            <end position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008734"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1261" mass="138690" checksum="EFBBE8EA4F65C88A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BD11</accession>
    <name>A0A673BD11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115430580</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>K(+)(in) + L-glutamate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-glutamate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BD11"/>
    <dbReference type="Ensembl" id="ENSSORT00005039300.1">
        <property type="protein sequence ID" value="ENSSORP00005038308.1"/>
        <property type="gene ID" value="ENSSORG00005017920.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BD11"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070779">
        <property type="term" value="P:D-aspartate import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140009">
        <property type="term" value="P:L-aspartate import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098712">
        <property type="term" value="P:L-glutamate import across plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3860.10:FF:000002">
        <property type="entry name" value="Amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF24">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <text evidence="15">Pore-forming (alpha) subunit of a voltage-gated delayed rectifier. Activates at more negative voltages, exhibits fast prepulse-independent activation kinetics and deactivates much more slowly, but shows no inactivation.</text>
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            <text>K(+)(in) = K(+)(out)</text>
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        <text evidence="16">Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv12.3/KCNH4 sub-subfamily.</text>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1117" mass="124911" checksum="FB336465FED4BCE7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BM51</accession>
    <name>A0A673BM51_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dopamine D1 receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115427449</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BM51"/>
    <dbReference type="Ensembl" id="ENSSORT00005042385.1">
        <property type="protein sequence ID" value="ENSSORP00005041323.1"/>
        <property type="gene ID" value="ENSSORG00005019232.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BM51"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001588">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gs"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF139">
        <property type="entry name" value="D(1A) DOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="39"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="345" mass="38342" checksum="76503558D33E45D4" modified="2020-06-17" version="1">MDLMNFTTVIDSGFLDEAPSSRVLTGCFLSLLILTTLLGNTLVCAAVTKFRHLRTKVTNLFVISLAVSDLLVAILVMPWKAVTEIAGFWPFGSFCDTWVAFDIMCSTASILNLCVISLDRYWAISSPFRYERKMTPKVAYVMIGVAWTLSVLISFIPVQLNWHKAQTTSYTSVTGSSGSLRSNATSYSPPENCDSSLNRTYAISTSLISFYIPVVIMVATYTQIYRIAHRQIRRISALERAAESAKNRHDSMGGGSSIAESESSFKMTFKRETKVLKTLSVIMGVFVCCWLPFFILNCMVPFCEKSSGGEAFPCINFRKAFSILLGCHRLYPGGHNMETVSLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNZ4</accession>
    <name>A0A673BNZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">methylated diphthine methylhydrolase</fullName>
            <ecNumber evidence="6">3.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dph7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphthine methyl ester-[translation elongation factor 2] + H2O = diphthine-[translation elongation factor 2] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42656"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="3.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH7 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.97" evidence="6"/>
    <dbReference type="RefSeq" id="XP_029999733.1">
        <property type="nucleotide sequence ID" value="XM_030143873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BNZ4"/>
    <dbReference type="FunCoup" id="A0A673BNZ4">
        <property type="interactions" value="1135"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044581.1">
        <property type="protein sequence ID" value="ENSSORP00005043485.1"/>
        <property type="gene ID" value="ENSSORG00005020083.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425973"/>
    <dbReference type="CTD" id="92715"/>
    <dbReference type="InParanoid" id="A0A673BNZ4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="225"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="441" mass="48219" checksum="7CB67144BC565135" modified="2020-06-17" version="1">MAWKSRTRSLQVFDTELSADTVEWCPVSPHHNILVCGTYQLQKGTGEEDATPSRTGRLYLFEFRREGPMSPPLTELQRMDTAAILDLKWCHVPLSEQAVLGLAAATGELQLYTLSDGQEGGCSLQSLSSVEVGVDRLALSLDWSTGRMDGSDVRLVCSDSAGCVSVLSLAEGALTVLSQWKAHDFEAWISAFSYWDTQLLYSGGDDCKLKGWDLRVGPSCPTFTSKRHSMGVCSIHSSPHREHILATGSYDEQVLLWDGRNMRQPLSETPMGGGVWRLKWHPSHQHLLLAACMHNDFHILHCQQALEGSGGACPIVASYILHNSLAYGADWSRLSLEEPAPCSPLTAEPKESLAEGGGHVRIQYESPTASFDTSLEDDAGRYIPEGITAPAASVAAGPTLSLDSDSPSLSCLLASCSFYDHMLHAWRWDWTPNAAQQEPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BP13</accession>
    <name>A0A673BP13_9TELE</name>
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        <submittedName>
            <fullName evidence="23">Dystonin</fullName>
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    </protein>
    <gene>
        <name evidence="23" type="primary">dst</name>
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    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
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        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Chromosome 15"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <location>
            <begin position="2955"/>
            <end position="2975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="6519"/>
            <end position="6528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="6529"/>
            <end position="6553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="6561"/>
            <end position="6571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="6572"/>
            <end position="6585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="6633"/>
            <end position="6643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="6658"/>
            <end position="6682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="6710" mass="759464" checksum="A676F104D26103FA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BP78</accession>
    <name>A0A673BP78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endophilin-B1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Endophilin-B2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">SH3 domain-containing GRB2-like protein B1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">SH3 domain-containing GRB2-like protein B2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115425989</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer, and heterodimer with SH3GLB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BP78"/>
    <dbReference type="Ensembl" id="ENSSORT00005044674.1">
        <property type="protein sequence ID" value="ENSSORP00005043575.1"/>
        <property type="gene ID" value="ENSSORG00005020089.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BP78"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11945">
        <property type="entry name" value="SH3_Endophilin_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028503">
        <property type="entry name" value="SH3GLB_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF68">
        <property type="entry name" value="DREBRIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="11 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="20">
        <location>
            <begin position="24"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="354"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="414" mass="46752" checksum="AD846157BC185FDB" modified="2020-06-17" version="1">MDFNVKKLASGAGVFFTRAVQFTEEKLGQAEKTELDAHFENLMSRADCTKNWTEKIYRQTEVLLQPNPSARIEEFLYEKLDRKAPSRITNAELLGQYMQDAAVEFGAGGPYGCTLIKVGECERRLGAAEREFLQTSAISFLTPLRNFLEGDWRTISKERRLLENLRLDLDACKTRLKKAKVAEAKAAMAPDFQETRPRNYVLSASASAFRFNYLYHIDSCHKWCVCSRPPPPNTRDRRALGCLKLPLKAEHELRVAQTEFDRQAEVTRLLLEGISSTHVNHLRCLNEFVEAQAAYYKQCHLHMQDLQKELGRLPNAFTLNSAHSMVTTASAPEEATSPVETDTLKIEEVQAPATGTRKAKVLYDYDAADSSELSLLADELITVYTMPGMDSDWLIGERGNQKGKVPVTYLELLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BW91</accession>
    <name>A0A673BW91_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Matrix metallopeptidase 20a (enamelysin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">mmp20a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="12">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BW91"/>
    <dbReference type="Ensembl" id="ENSSORT00005045980.1">
        <property type="protein sequence ID" value="ENSSORP00005044847.1"/>
        <property type="gene ID" value="ENSSORG00005020622.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BW91"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF297">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="15">
        <location>
            <begin position="110"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="14">
        <location>
            <begin position="339"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="14">
        <location>
            <begin position="386"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="12">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="173"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="175"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
        </ligand>
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        <location>
            <position position="180"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>3</label>
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            <name>Ca(2+)</name>
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        <location>
            <position position="188"/>
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            <label>1</label>
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            <label>2</label>
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            <label>2</label>
        </ligand>
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        <location>
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            <name>Zn(2+)</name>
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            <label>1</label>
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            <label>3</label>
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            <name>Zn(2+)</name>
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        </ligand>
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            <name>Zn(2+)</name>
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            <name>Zn(2+)</name>
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            <name>Zn(2+)</name>
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            <name>Ca(2+)</name>
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        </ligand>
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            <name>Ca(2+)</name>
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        </ligand>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
    </feature>
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        <location>
            <begin position="294"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="466" mass="53321" checksum="BB142ECE74F2E8EB" modified="2025-10-08" version="2">MNCTPSQGVCLFTYKTKTETKPPLGPFRNSTQLQQRENYLTKFYHLNPRGSSRLRARRTRSTSDMEDKIRQMQHFFSLTESGHLDQRTLDVMKEARCGVPDVENFSFYPNKPKWKSNTITYTIGRYTPDMKREDVEKSFRLALKMWSDATPLKFIKVNHGKADIVLSFARGTHGDFFPFDGPRGVLAHAFQPGEGIGGDVHFDEDETWATGSQGFLFAVAAHELGHSLGLTHSRDPSAIMYPNYRRHSRTQYSLSKDDILGIQTLYGEQNGNFVALLFCFLGKPKENKMIPKKCDPSFSFDAAALIGNEIVFFKNRTTWTTYWNRLREGPSSTYLPSVHSHIDAAYDIPAKGVAYIFTGHKYWVVQQLKMKSHAGSIYEYGFPSSVRQVDAAVHVSEYGKTIFFTGELYYRYDEQKKKMDPDINEFLVCFLLTGSIFILSGTKSYQYDFRKNRVVKTISANSWLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BWL0</accession>
    <name>A0A673BWL0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Thymidine kinase 2, mitochondrial</fullName>
            <ecNumber evidence="3">2.7.1.21</ecNumber>
            <ecNumber evidence="11">2.7.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">2'-deoxyuridine kinase TK2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Deoxycytidine kinase TK2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">tk2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2'-deoxycytidine + ATP = dCMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46040"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:46041"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2'-deoxyuridine + ATP = dUMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28206"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:28207"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>thymidine + ATP = dTMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19129"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19130"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCK/DGK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.21" evidence="3"/>
    <dbReference type="EC" id="2.7.1.74" evidence="11"/>
    <dbReference type="RefSeq" id="XP_030011912.1">
        <property type="nucleotide sequence ID" value="XM_030156052.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030011913.1">
        <property type="nucleotide sequence ID" value="XM_030156053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BWL0"/>
    <dbReference type="Ensembl" id="ENSSORT00005048475.1">
        <property type="protein sequence ID" value="ENSSORP00005047301.1"/>
        <property type="gene ID" value="ENSSORG00005021626.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434229"/>
    <dbReference type="CTD" id="7084"/>
    <dbReference type="InParanoid" id="A0A673BWL0"/>
    <dbReference type="OrthoDB" id="567086at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004137">
        <property type="term" value="F:deoxycytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004797">
        <property type="term" value="F:thymidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000998">
        <property type="entry name" value="Thymidine kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF24">
        <property type="entry name" value="THYMIDINE KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="18">
        <location>
            <begin position="74"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="282" mass="32011" checksum="C7C92EFE142369B5" modified="2020-06-17" version="1">MPGTATRGMLFGRCVKLLCAHGARSSSLSPSVAVWTKGLRTPAAATFTPGQSRAVRTAVTGKLVRNGEDKKAVICIEGNIASGKTTCLEYFSKTSNIEVLTEPVSKWRNVRGHNPLALMYQDPERWGITLQTYVQLTMLDRHLSSIAAPIRMMERSIFSAKYIFVENLYRSGKMPEVDYAVLSEWFDWITTNISIPVDLIVYLQTSPETCHERLKQRCREEEKVIPLEYLESIHQLYEDWLIKRTSAPLPAPVLVIPADHDLQKMLHHYEENREKILSASYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXA4</accession>
    <name>A0A673BXA4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Dedicator of cytokinesis 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dock2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030001113.1">
        <property type="nucleotide sequence ID" value="XM_030145253.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046378.1">
        <property type="protein sequence ID" value="ENSSORP00005045242.1"/>
        <property type="gene ID" value="ENSSORG00005013740.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426957"/>
    <dbReference type="CTD" id="1794"/>
    <dbReference type="InParanoid" id="A0A673BXA4"/>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11706">
        <property type="entry name" value="DHR2_DOCK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000004">
        <property type="entry name" value="Dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000004">
        <property type="entry name" value="Dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000044">
        <property type="entry name" value="dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.350:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000057">
        <property type="entry name" value="Dedicator of cytokinesis protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.350">
        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026799">
        <property type="entry name" value="DHR2_DOCK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042455">
        <property type="entry name" value="DOCK_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF6">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="8"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="12">
        <location>
            <begin position="423"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="13">
        <location>
            <begin position="1215"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="567"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1653"/>
            <end position="1687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1738"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1777"/>
            <end position="1838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1743"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1782"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1820"/>
            <end position="1838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1838" mass="212011" checksum="62617DE83728274D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0G1</accession>
    <name>A0A673C0G1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Parathyroid hormone/parathyroid hormone-related peptide receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">PTH/PTHrP type I receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Parathyroid hormone 1 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">pth3r</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity. PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C0G1"/>
    <dbReference type="Ensembl" id="ENSSORT00005049898.1">
        <property type="protein sequence ID" value="ENSSORP00005048706.1"/>
        <property type="gene ID" value="ENSSORG00005022185.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C0G1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004991">
        <property type="term" value="F:parathyroid hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15265">
        <property type="entry name" value="7tmB1_PTHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000070">
        <property type="entry name" value="Parathyroid hormone/parathyroid hormone-related peptide receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002170">
        <property type="entry name" value="GPCR_2_parathyroid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF18">
        <property type="entry name" value="PARATHYROID HORMONE_PARATHYROID HORMONE-RELATED PEPTIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00393">
        <property type="entry name" value="PTRHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="20">
        <location>
            <begin position="59"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="21">
        <location>
            <begin position="139"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="508"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="558"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="578" mass="65448" checksum="998F62F4ABAAA41B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C805</accession>
    <name>A0A673C805_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ccny</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin Y subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C805"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cyclin_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="186"/>
            <end position="271"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="40990" checksum="F165B63D8117D123" modified="2020-06-17" version="1">MGNTTSCCVSSSPKHRRNNHSRLEPYRPEPELSREDTGCNLQHISDRENIDDLPMEYNPSDHPRASTIFLSKSQTDVREKRKSLYINHHHPGPVRRKYSSCSTIFLDDSTVSQPNLKYTIKCHVSVDMELFLFLTQTDVFLSVCFSREVDGRMLLDIFDEKQHPLSKSEVPPDYDKHDPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRDKAQKLEAISRLCDDKYKDLRKLAKKRSVSADNLTVVRWCPAIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CE91</accession>
    <name>A0A673CE91_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhotekin 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CE91"/>
    <dbReference type="Ensembl" id="ENSSORT00005055165.1">
        <property type="protein sequence ID" value="ENSSORP00005053896.1"/>
        <property type="gene ID" value="ENSSORG00005024197.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CE91"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000915">
        <property type="term" value="P:actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012966">
        <property type="entry name" value="AHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051364">
        <property type="entry name" value="Cytokinesis/Rho-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538">
        <property type="entry name" value="ANILLIN/RHOTEKIN RTKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538:SF19">
        <property type="entry name" value="RHOTEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08174">
        <property type="entry name" value="Anillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="REM-1" evidence="1">
        <location>
            <begin position="17"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="319" mass="35356" checksum="1B5186F67D6185EC" modified="2020-06-17" version="1">QILFRQKFRIYVIYHIFTDEEVLQQIEREVRMRDGASKLLAACSQREQALEASKNLLTCNARILALLSQLQKMRKAQILQRVGNRSGHKIVTCTGKLAFSDLRIPLMWKDSEYFKNKGELHRCAVFCLLQCGSEIYDTDLQMVDRTLTDICFEDTIVFSNDVGPEFQLRVELYSSCAAEDFFPGAAGPRRGSHSGGSAMGSFTGKKIRAVFESAAVCGSMSSGGDAGPGSASPSLCPDLSALGPKFNLLAHTTLTLSHVQERFKTHDLSLLAAEESPFWLPLYGNMCCRLVAQPLCMLQAGISGQLQIKVTRFFILLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFG9</accession>
    <name>A0A673CFG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">anxa1a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CFG9"/>
    <dbReference type="FunCoup" id="A0A673CFG9">
        <property type="interactions" value="557"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052054.1">
        <property type="protein sequence ID" value="ENSSORP00005050838.1"/>
        <property type="gene ID" value="ENSSORG00005022687.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CFG9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004859">
        <property type="term" value="F:phospholipase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071385">
        <property type="term" value="P:cellular response to glucocorticoid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000007">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002390">
        <property type="entry name" value="ANX3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF17">
        <property type="entry name" value="ANNEXIN A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00199">
        <property type="entry name" value="ANNEXINIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="377" mass="42401" checksum="E83E6143C5E1D5DE" modified="2020-06-17" version="1">MCRCTLNVILTLPKSYSVAEKFKKIPFATLYKQCHFMHATGFRSNIYKTHNNLCYVSQVLKKEGTVTAAPNFSPSGDAAVLDKAIKAKGVDENTIIEILVKRSNEQRQQIKEAYQQASGKPLESALKNALKGDLEDVVLALLKTPAQYDAQQLKQAMKGLGTDEDTLIEILASRTNRELEEVKKAYKAEYKKELEDDIKSDTSGDFKKALIELCCEDTRSTDDWVSEPLIDSDARALYEAGEGRKGKDCSVFIEILTTRSAAHLRKVFERYSKYSKVDVAQAIDLEMKGDIESCLTAVVKCAGSRPAFFAEKLYLAMKGKGTRKNILTRIMVSRSEIDMIRIKEEYKKNYGKTLYQDILDDTKGDYEKILLALCGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJA6</accession>
    <name>A0A673CJA6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">septin6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJA6"/>
    <dbReference type="Ensembl" id="ENSSORT00005056966.1">
        <property type="protein sequence ID" value="ENSSORP00005055681.1"/>
        <property type="gene ID" value="ENSSORG00005024517.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJA6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000036">
        <property type="entry name" value="septin-6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025552044" description="Septin" evidence="7">
        <location>
            <begin position="25"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Septin-type G" evidence="8">
        <location>
            <begin position="47"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="329"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="448" mass="51721" checksum="1704FA2D6376920D" modified="2020-06-17" version="1">MNSVIYHICTWWLVTVSTGEGARAVPLAGHVGFDSMPDQLVNKSVNHGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPTQHNQPGVTLKSNTYELQESNVRLKLTVVNTVGFGDQINKEDSYKSIVEFIDAQFEAYLQEELKIKRTLHSYHDTRIHACLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKSDAISKSELAKFKIKITSELVSNGVQIYQFPTDDESVAEINSTMNSHLPFAVVGSTEEVKIGNKMVKARQYPWGTVQVENENHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFMGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEEKKKTLDDELNMFKQKKTAAELLQNQAQQAGGSTTLKRDKERKKNKEHMCTWILLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CL62</accession>
    <name>A0A673CL62_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formin-like 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fmnl2a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CL62"/>
    <dbReference type="Ensembl" id="ENSSORT00005057652.1">
        <property type="protein sequence ID" value="ENSSORP00005056356.1"/>
        <property type="gene ID" value="ENSSORG00005024876.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CL62"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000001">
        <property type="entry name" value="Formin-like 1, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000045">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF5">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="4">
        <location>
            <begin position="22"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="5">
        <location>
            <begin position="587"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="507"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="970"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="363"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="858"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="511"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="559"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1087" mass="123518" checksum="F522B30FEF909C52" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CN41</accession>
    <name>A0A673CN41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Protein pelota homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pelo</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, pelo recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030019273.1">
        <property type="nucleotide sequence ID" value="XM_030163413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CN41"/>
    <dbReference type="FunCoup" id="A0A673CN41">
        <property type="interactions" value="1253"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055257.1">
        <property type="protein sequence ID" value="ENSSORP00005053987.1"/>
        <property type="gene ID" value="ENSSORG00005024237.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439553"/>
    <dbReference type="CTD" id="53918"/>
    <dbReference type="InParanoid" id="A0A673CN41"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000001">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="385" mass="43269" checksum="C5488BFAD870FC14" modified="2020-06-17" version="1">MKLIHKDIEKDNAGQVTLVPEEAEDMWHTYNLLQVGDSLRASTIRKVQTESNTGSVGSSRVRTTLTICVETIDFDSQACQLRVKGTNLEENQYVKMGAYHTIELELNRKFTLAKKSWDSVVLDRIEQACDVTQKADVAAVVMQEGLANLVLVTPAMTLLRAKVEVTIPRKRRGSCTQHEKALERFYEAVMQAILRHINFDVVKCILVASPGFVKDQFIAYLFKEAVRQDNKTLLENRPKFMLVHSSSGHKYSLKEILSDPTVTSRLSDTKAAGEVKALEDFYKMLQHEPDRAFYGLAHVEKATDALAVDTLLISDKLFRHQDVPTRSRYVRLVDNVRDNGGNVRIFSSLHVSGEQLTQLSGVAAILRFPIADLSEPEDDSSSDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CNM0</accession>
    <name>A0A673CNM0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cocaine- and amphetamine-regulated transcript protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115425899</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CART family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029999615.1">
        <property type="nucleotide sequence ID" value="XM_030143755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CNM0"/>
    <dbReference type="Ensembl" id="ENSSORT00005056215.1">
        <property type="protein sequence ID" value="ENSSORP00005054939.1"/>
        <property type="gene ID" value="ENSSORG00005024570.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425899"/>
    <dbReference type="InParanoid" id="A0A673CNM0"/>
    <dbReference type="OrthoDB" id="9936511at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008343">
        <property type="term" value="P:adult feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032099">
        <property type="term" value="P:negative regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.30">
        <property type="entry name" value="CART, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009106">
        <property type="entry name" value="CART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036722">
        <property type="entry name" value="CART_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655">
        <property type="entry name" value="COCAINE AND AMPHETAMINE REGULATED TRANSCRIPT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655:SF5">
        <property type="entry name" value="COCAINE- AND AMPHETAMINE-REGULATED TRANSCRIPT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06373">
        <property type="entry name" value="CART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64546">
        <property type="entry name" value="Satiety factor CART (cocaine and amphetamine regulated transcript)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025429309" evidence="5">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="112" mass="12611" checksum="BAF207E9C2F24939" modified="2020-06-17" version="1">MGSWCVYVFCTLLCAAGLLYSHGLVETEEYKTKYSTYFTATDKGKEKQLISDLHGVLERLQNNPFPALKKKHGYLPMCDPGDQCALRKGSRIGKLCDCALPRTCNSFLHRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CPJ5</accession>
    <name>A0A673CPJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pleckstrin homology domain containing, family G (with RhoGef domain) member 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">plekhg7</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029992457.1">
        <property type="nucleotide sequence ID" value="XM_030136597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CPJ5"/>
    <dbReference type="FunCoup" id="A0A673CPJ5">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058880.1">
        <property type="protein sequence ID" value="ENSSORP00005057561.1"/>
        <property type="gene ID" value="ENSSORG00005025511.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420952"/>
    <dbReference type="CTD" id="440107"/>
    <dbReference type="InParanoid" id="A0A673CPJ5"/>
    <dbReference type="OrthoDB" id="5585231at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13245">
        <property type="entry name" value="PH_PLEKHG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040181">
        <property type="entry name" value="PKHG5/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217:SF6">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15720">
        <property type="entry name" value="DUF4675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="329"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="573"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="730" mass="83281" checksum="18082BAE821DFCB1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CR38</accession>
    <name>A0A673CR38_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lens intrinsic membrane protein 2.2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PMP-22/EMP/MP20 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CR38"/>
    <dbReference type="Ensembl" id="ENSSORT00005055727.1">
        <property type="protein sequence ID" value="ENSSORP00005054453.1"/>
        <property type="gene ID" value="ENSSORG00005024394.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CR38"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004032">
        <property type="entry name" value="PMP22_EMP_MP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF106">
        <property type="entry name" value="LENS INTRINSIC MEMBRANE PROTEIN 2.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01221">
        <property type="entry name" value="PMP22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="185" mass="20676" checksum="40A3DFAC90D4FF1B" modified="2020-06-17" version="1">MLYTLAGGGTLCGVAALVLLIVSTATDFWMQYRYSGSSANQGLWRFCINHKCHAHTITVGEHLLFSHILNITTFWDATRAFMLLSVLSCFAGVVLGLSAFTNGTKNRRVLFFLKSLFFLSGFLALLALAIYTGVTVTFFGKRFVDWRFSWSYIIGWVAIILAFAAGILYFSLTHKDQLPVTQTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRY5</accession>
    <name>A0A673CRY5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">vitamin-K-epoxide reductase (warfarin-sensitive)</fullName>
            <ecNumber evidence="3">1.17.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">vkorc1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VKOR family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673CRY5"/>
    <dbReference type="FunCoup" id="A0A673CRY5">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057397.1">
        <property type="protein sequence ID" value="ENSSORP00005056104.1"/>
        <property type="gene ID" value="ENSSORG00005024984.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CRY5"/>
    <dbReference type="OrthoDB" id="17010at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047057">
        <property type="term" value="F:vitamin-K-epoxide reductase (warfarin-sensitive) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042373">
        <property type="term" value="P:vitamin K metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12917">
        <property type="entry name" value="VKOR_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.130:FF:000001">
        <property type="entry name" value="Vitamin K epoxide reductase complex subunit 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.130">
        <property type="entry name" value="VKOR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012932">
        <property type="entry name" value="VKOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038354">
        <property type="entry name" value="VKOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042406">
        <property type="entry name" value="VKORC1/VKORC1L1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14519:SF8">
        <property type="entry name" value="VITAMIN K EPOXIDE REDUCTASE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14519">
        <property type="entry name" value="VITAMIN K EPOXIDE REDUCTASE COMPLEX, SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07884">
        <property type="entry name" value="VKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00756">
        <property type="entry name" value="VKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0874">Quinone</keyword>
    <keyword evidence="11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Vitamin K epoxide reductase" evidence="13">
        <location>
            <begin position="10"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="169" mass="18582" checksum="FF435F93A4F863D6" modified="2020-06-17" version="1">MSVGGTQGLPTWERKVRIFLCVFGFVLSIYALHVELSKERNPEYRAMCDLGESVSCSKVFTSRWGRGFGLVQFFVGKDSPLNQPNSVLGIIFYTLQLGLGLSLSKKAAVFLVLSSWVSVAGSLYLASILAFVLGDFCMVCVSTYIINFALLFTNLKRRGAIEGMKEKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSK2</accession>
    <name>A0A673CSK2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RING finger protein 11-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rnf11a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CSK2"/>
    <dbReference type="Ensembl" id="ENSSORT00005059959.1">
        <property type="protein sequence ID" value="ENSSORP00005058631.1"/>
        <property type="gene ID" value="ENSSORG00005025864.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSK2"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16468">
        <property type="entry name" value="RING-H2_RNF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042981">
        <property type="entry name" value="RNF11_RING-H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052804">
        <property type="entry name" value="UEC_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359">
        <property type="entry name" value="GEO07743P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="92"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="147" mass="16396" checksum="6A9D4F6163E88574" modified="2020-06-17" version="1">MGNCLFSQGADDLSLLNDSEGGSLPGEPPPPYQERAEPVPVYHPTPGESRLASQLTEEEQIRIAQRIGLIQHLPRGIFDPGSDPSDKKVKECVICMMDFEYGDPVRFLPCLHIYHVDCIDPWLMRSFTCPTCMEPVDAALLSTYETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSP4</accession>
    <name>A0A673CSP4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WxxW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CSP4"/>
    <dbReference type="Ensembl" id="ENSSORT00005056847.1">
        <property type="protein sequence ID" value="ENSSORP00005055562.1"/>
        <property type="gene ID" value="ENSSORG00005024799.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSP4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039675">
        <property type="entry name" value="CILP1/CILP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025155">
        <property type="entry name" value="WxxW_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031:SF4">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13330">
        <property type="entry name" value="Mucin2_WxxW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="WxxW" evidence="5">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="WxxW" evidence="5">
        <location>
            <begin position="79"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="166" mass="18517" checksum="C691648735579E34" modified="2020-06-17" version="1">ETLSSLRKENPGKICPNPTDIEVQTVTGQSLAAAGEVIYKADTTSGFICRNEDQTDKQCTDYRVRFSCPPSYCGFGACWTQWFDRDDPSGTGDWETLSSLRAAYPNKICKTPMYIEAVVVGTNFPASITGEVFHIFNPTEGFVCRKTDQKDKKCLDYKVRFGCCCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CST7</accession>
    <name>A0A673CST7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115430340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/PBX homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CST7"/>
    <dbReference type="Ensembl" id="ENSSORT00005057731.1">
        <property type="protein sequence ID" value="ENSSORP00005056434.1"/>
        <property type="gene ID" value="ENSSORG00005025100.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CST7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000008">
        <property type="entry name" value="Pre-B-cell leukemia transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005542">
        <property type="entry name" value="PBX_PBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03792">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51978">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PBC" evidence="10">
        <location>
            <begin position="41"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="233"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="235"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="438" mass="48324" checksum="BC6C83F66597036F" modified="2020-06-17" version="1">MEDQGRMMQALSAVTLAGHSAHTGAVGLPLPHIHDGADGDGRKQDIGSILHQIMTITDQSLDEAQAKKHGLNCHRMKPALFSVLCEIKEKTGLSIRGVQEEDPPDAQLMRLDNMLLAEGVAGPEKGGGAAVAAAAAAAAGSITDNSIEHSDYRAKLTQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVNIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFSKQATEILNEYFYSHLSNPYPSEEAKEELAKKCSITVSQVSRNTLDVSNWFGNKRIRYKKNIGKFQEEANLYAAKTAVTATQAVAAAVHNSQDNSPSTPNTGSPGSFSLPHSGDMFISMQGLNGASYQGAQVGANMQSQVGAVYDKRSGNAFSILMCLWSLGGFFEYKMCIHFQIFLDNVLQTNFTSSNKKVCNVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSZ9</accession>
    <name>A0A673CSZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein Pegasus</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Ikaros family zinc finger protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ikzf5</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Ikaros C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CSZ9"/>
    <dbReference type="FunCoup" id="A0A673CSZ9">
        <property type="interactions" value="1592"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058782.1">
        <property type="protein sequence ID" value="ENSSORP00005057465.1"/>
        <property type="gene ID" value="ENSSORG00005025476.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSZ9"/>
    <dbReference type="OrthoDB" id="5576026at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000402">
        <property type="entry name" value="IKAROS family zinc finger 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000924">
        <property type="entry name" value="IKAROS family zinc finger 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001097">
        <property type="entry name" value="IKAROS family zinc finger 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF55">
        <property type="entry name" value="ZINC FINGER PROTEIN PEGASUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="305"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="341"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="423" mass="46921" checksum="20DCC280104365F1" modified="2020-06-17" version="1">MGEEKPDTLDFVKDFQEYLSQQTQHVNMISGSVSGVKEADELPADCSQNGLDHPSVDMSLEDSSGILVDGFERTYDGKLKCRYCNYATRGTARLIEHIRIHTGEKPHRCHLCPFASAYERHLEAHMRSHTGEKPYKCELCSFRCSDRSNLSHHRRRRHKLLPMKGARSLSHKKMLSVLQKKASSLGYGRRLLINFSPPSMVVHKADNVSDYSHELPHLRQETYDNQSRAGEDGISANQNHHHHDMVMDNPLNQLSTLAGQLASLPSESQAQTQPPVSPGAESIVDEKPFLIQQPHPATAPVAVTASMAHASSSSPITPEPRAPPHSNCSPGGGPCSEHSGRISTPSISNSQPSTPAPGLSAPLQDPHMLHHCQHCDIYFPDNILYTIHMGCHGYENPFQCNICGHKCKSKYDFACHFARGQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CTT0</accession>
    <name>A0A673CTT0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CTT0"/>
    <dbReference type="Ensembl" id="ENSSORT00005056628.1">
        <property type="protein sequence ID" value="ENSSORP00005055348.1"/>
        <property type="gene ID" value="ENSSORG00005024701.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CTT0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47135">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47135:SF3">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="107" mass="11678" checksum="C8BE9C92D14821A6" modified="2020-06-17" version="1">TGPCALTSISAVTQCNSNKILVEWELSENSPIYVVTAEGHDQTYITCNSTTSSCELQDVQCGTQYSIIVATSSDKCSSLRSPPKRIKTGINVCAFICLFVPCHVRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CVE8</accession>
    <name>A0A673CVE8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CVE8"/>
    <dbReference type="Ensembl" id="ENSSORT00005057227.1">
        <property type="protein sequence ID" value="ENSSORP00005055938.1"/>
        <property type="gene ID" value="ENSSORG00005024923.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CVE8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025631393" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="23"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="128" mass="14293" checksum="45EB7EAE77747C4A" modified="2020-06-17" version="1">MLLLVSLVSFVFHSAYTDANILPSVSIPASPTVSVPQQWVVLERESHLKCHAVGFYPPPVSFSWIRDGREIQPPYQVEGEQTADGFYTAEANLTFWPSRDDQNVTFGCKVSHNASNVLADMKLKQQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CVW0</accession>
    <name>A0A673CVW0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">suz12b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CVW0"/>
    <dbReference type="FunCoup" id="A0A673CVW0">
        <property type="interactions" value="1526"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059906.1">
        <property type="protein sequence ID" value="ENSSORP00005058580.1"/>
        <property type="gene ID" value="ENSSORG00005025835.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CVW0"/>
    <dbReference type="OrthoDB" id="166746at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21740">
        <property type="entry name" value="C2_II_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21551">
        <property type="entry name" value="VEFS-box_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21750">
        <property type="entry name" value="ZnB-Zn_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019135">
        <property type="entry name" value="Polycomb_protein_VEFS-Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057540">
        <property type="entry name" value="Znf_SUZ12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF0">
        <property type="entry name" value="POLYCOMB PROTEIN SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09733">
        <property type="entry name" value="VEFS-Box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23320">
        <property type="entry name" value="Zn_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Polycomb protein SUZ12-like zinc finger" evidence="11">
        <location>
            <begin position="392"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Polycomb protein VEFS-Box" evidence="10">
        <location>
            <begin position="518"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="222"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="333"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="639"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="693" mass="78747" checksum="46A4B3AFB1EA2AD2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CZ54</accession>
    <name>A0A673CZ54_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MID1 interacting protein 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mid1ip1a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPOT14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CZ54"/>
    <dbReference type="Ensembl" id="ENSSORT00005058867.1">
        <property type="protein sequence ID" value="ENSSORP00005057548.1"/>
        <property type="gene ID" value="ENSSORG00005025505.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:regulation of lipid biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Lipogen_MT_Stabilize_sf"/>
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        <property type="entry name" value="Spot_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14315:SF19">
        <property type="entry name" value="MID1-INTERACTING PROTEIN 1-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14315">
        <property type="entry name" value="SPOT14 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07084">
        <property type="entry name" value="Spot_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="131" mass="15198" checksum="FCD738476752DC3D" modified="2020-06-17" version="1">MMQLPETPTQKNNLFNAMNRFIGAVNNMDQTIMVPSLLRDVPLDEDKASLPLRSYEDEGDVYSYYQLLKSIRRDIEWGVRCAEEYEEDEDLEKQFQYHLTGLQGVLSKLTQQANCVTKRYKQEIGIGGWGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4Z4</accession>
    <name>A0A672Y4Z4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y4Z4"/>
    <dbReference type="Ensembl" id="ENSSORT00005001512.1">
        <property type="protein sequence ID" value="ENSSORP00005001467.1"/>
        <property type="gene ID" value="ENSSORG00005000906.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4Z4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000033">
        <property type="entry name" value="Immunodeficiency virus type I enhancer binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000594">
        <property type="entry name" value="Transcription factor HIVEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051969">
        <property type="entry name" value="Zinc-finger_DNA-bd_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944:SF6">
        <property type="entry name" value="HIVEP ZINC FINGER 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944">
        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="55"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="165"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="225" mass="25004" checksum="8881C659BF1015F8" modified="2020-06-17" version="1">MGYFRSAWQRTSSAACDIIELTFFIPPLYPLFSQTRENYDSHIDSDTLLDQKHEATKATTVSSLSPGTPPSTASGPNQPKPDTQEGTPKQKRERKPQRPGKYVCTYCGRACAKPSVLQKHIRSHTGERPYPCAPCGFSFKTKSNLYKHRKSHTHRVKAGLALGETRPLEEQITESEDETRQLSSVSSNTERQGGVTSIKSHEPEKAKDCTGGNDDSYAVKKRLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5A1</accession>
    <name>A0A672Y5A1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calpastatin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Calpain inhibitor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I27 (calpastatin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y5A1"/>
    <dbReference type="Ensembl" id="ENSSORT00005001665.1">
        <property type="protein sequence ID" value="ENSSORP00005001617.1"/>
        <property type="gene ID" value="ENSSORG00005001009.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y5A1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010859">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026998">
        <property type="entry name" value="Calpastatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001259">
        <property type="entry name" value="Prot_inh_calpain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10077">
        <property type="entry name" value="CALPASTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10077:SF0">
        <property type="entry name" value="CALPASTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00748">
        <property type="entry name" value="Calpain_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0789">Thiol protease inhibitor</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="139" mass="15317" checksum="8556E52B8E2C4580" modified="2020-06-17" version="1">MSSEALSALGDMLGEPEPVPESPKLRPEDIVSEDKLKKEKGVRVGERDDTLPPEYRFNEEELKKLPAPKPEPKMDTTEALDILSGDFTTPSAPPAQVLSKCMTTRPEGIVQCLQPDFMLLLLCVVDESYTLCRPPCGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y807</accession>
    <name>A0A672Y807_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Axin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115427822</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1588" mass="172116" checksum="827BD0F0BB267EBD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAA7</accession>
    <name>A0A672YAA7_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Chromosome 4 open reading frame 54</fullName>
        </submittedName>
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    <gene>
        <name evidence="3" type="primary">LOC115422673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSSORT00005001163.1">
        <property type="protein sequence ID" value="ENSSORP00005001129.1"/>
        <property type="gene ID" value="ENSSORG00005000675.1"/>
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    <dbReference type="InParanoid" id="A0A672YAA7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="CEFIP"/>
    </dbReference>
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        <property type="entry name" value="DUF4585"/>
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        <property type="entry name" value="CARDIAC-ENRICHED FHL2-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33775:SF4">
        <property type="entry name" value="CHROMOSOME 4 OPEN READING FRAME 54"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF4585"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1233"/>
            <end position="1290"/>
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        <location>
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            <begin position="434"/>
            <end position="478"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="700"/>
        </location>
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        <location>
            <begin position="730"/>
            <end position="753"/>
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        <location>
            <begin position="856"/>
            <end position="881"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1169"/>
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            <begin position="1337"/>
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            <begin position="64"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
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            <begin position="443"/>
            <end position="472"/>
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            <begin position="599"/>
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        <location>
            <begin position="658"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="669"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="690"/>
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        <location>
            <begin position="866"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1175"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="1339"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1353"/>
            <end position="1363"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF15232"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1431" mass="156052" checksum="41BFA5C4C35F0BB6" modified="2020-06-17" version="1"> </sequence>
</entry>
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    <accession>A0A672YC87</accession>
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        <submittedName>
            <fullName evidence="8">Protein unc-119 homolog B-like</fullName>
        </submittedName>
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        <name evidence="8" type="primary">LOC115418820</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDE6D/unc-119 family.</text>
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        <property type="nucleotide sequence ID" value="XM_030133309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YC87"/>
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        <property type="protein sequence ID" value="ENSSORP00005001200.1"/>
        <property type="gene ID" value="ENSSORG00005000716.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418820"/>
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    <dbReference type="OrthoDB" id="10248777at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:lipoprotein transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="protein unc-119 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.40">
        <property type="entry name" value="GMP phosphodiesterase, delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051519">
        <property type="entry name" value="PDE6D_unc-119_myristoyl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037036">
        <property type="entry name" value="PDED_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951:SF3">
        <property type="entry name" value="PROTEIN UNC-119 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951">
        <property type="entry name" value="RETINAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="7">
        <location>
            <begin position="84"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="243" mass="27742" checksum="A7AE87B78582D610" modified="2020-06-17" version="1">MSGAKARSDAPVAENVPGGGHGTAAPPRDRKPGGGVLKRLKSRRSQVDSRPVTEEELRTQSGHISPEDVLGLRVATRGYLCKPEENVYNIDFVRFKIRDLETSTVLFEIAKPPHAEDDEENREGDASVGRFVRYQFTPAFLRLRTVGATVEFTVGNRPLNNFRMIERHYFRDHLLKSFDFDFGFCIPNSRNTCEHIYEFPQLSESLVRQMVECPYETRSDSFYFVENRLVMHNKADYAYNGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCF8</accession>
    <name>A0A672YCF8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Anti-apoptotic protein NR13-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bcl2l10</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bcl-2 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A672YCF8"/>
    <dbReference type="FunCoup" id="A0A672YCF8">
        <property type="interactions" value="2"/>
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    <dbReference type="Ensembl" id="ENSSORT00005002704.1">
        <property type="protein sequence ID" value="ENSSORP00005002629.1"/>
        <property type="gene ID" value="ENSSORG00005001614.1"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF61">
        <property type="entry name" value="BCL2-LIKE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="4">
        <location>
            <begin position="60"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="210" mass="23077" checksum="2132548907535917" modified="2020-06-17" version="1">MKRGSPSAVLMCREQTDIAGRKMSCGLWKETLAMAEDYMALCCTGPQAAPPPPSESAAAMRLLAQDVERQHQARFLSLAQTFLRQCGPEPCSSLRKVMEELVGDGHLNWGRVVSLFTFTGVLARQLMEQKGTKPGLDPQQVPGNYRGLAETIADYLGEEKKDWLLENDGWEGFCKFSHHARKVSQDSSMKKALFAAAGVGLAGLTFLLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDV2</accession>
    <name>A0A672YDV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">acvrl1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672YDV2"/>
    <dbReference type="Ensembl" id="ENSSORT00005001281.1">
        <property type="protein sequence ID" value="ENSSORP00005001246.1"/>
        <property type="gene ID" value="ENSSORG00005000735.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDV2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070724">
        <property type="term" value="C:BMP receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23534">
        <property type="entry name" value="TFP_LU_ECD_ALK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000064">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF66">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RECEPTOR R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="16 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025429533" description="receptor protein serine/threonine kinase" evidence="24">
        <location>
            <begin position="27"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="26">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="204"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="509" mass="57786" checksum="678E994E5308F9A3" modified="2020-06-17" version="1">MGSSALLTMVIAVLFLWISAFHAVSASDCKDDDDGKLLCTCENEKGTCVNGTCRGDVCFYSWWRGIEERGCFSNDNYREQCYSAFHLLFIQCCRENECNAFITVPPRINQEPTPNPPVPPRPELWIAVSLLLLTVAACVCGVVLFLRCRRAHCRLKNMEDPDATMLKVPNGDDPTYGDIFDEFCTSGSGTGLPYLVQRTMARQISLVECVGKGRYGEVWRGTWMGESVAVKIFSSRDEQSWFRETEIYNTVQLRHDNILGFIASDMTSKNSSTQLWLVTHFHELGSLYDFLQYSSLEPESCLRMCLSVACGLVHLHTEIVSSQGKPAIAHRDLKSRNILVKWNGQCCIADLGLAVIHSQSNDYLDVGNNPRVGTKRYMAPEVLDETIRVDVFESYKQTDIWALGLVFWEISRRTNVNGIVEEYRPPFFDMVPSDPSFEEMKKVVCVDQQRPSLHNRLHSHPILTAIVKIMKECWFQSPSARLTALRVRKTLSKLDHDSDFSIEKLKRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGN3</accession>
    <name>A0A672YGN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Adenylate cyclase-inhibiting G alpha protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">gnai3</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the G-alpha family. G(i/o/t/z) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YGN3"/>
    <dbReference type="Ensembl" id="ENSSORT00005003848.1">
        <property type="protein sequence ID" value="ENSSORP00005003739.1"/>
        <property type="gene ID" value="ENSSORG00005002191.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YGN3"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007212">
        <property type="term" value="P:G protein-coupled dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002487">
        <property type="entry name" value="Guanine nucleotide-binding protein G(i) subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003559">
        <property type="entry name" value="Guanine nucleotide-binding protein G(i) subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF230">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12 20" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="175"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="40807" checksum="9D3BE15454ED435F" modified="2020-06-17" version="1">MGCTISAEDKAAVERSKMIDKNLREDREKSSREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECKQYKVVVYSNTIQSIMAIIRAMGRLKIDFGDPARADDARQLFALASSAEEGVMSAELTRVIRRLWSDEGVQACFDRSREYQLNDSAAYYLNDLDRICQTNYVPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTLTSIILFLNKKDLFEEKISRSPLTICYPEYTGGHAYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKFNLREIGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJA2</accession>
    <name>A0A672YJA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJA2"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04981">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001532">
        <property type="entry name" value="Immunoglobulin heavy variable 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
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    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="101" mass="11322" checksum="FB93BD79F4B53023" modified="2020-06-17" version="1">GQTLTESGPAVKRPGESHRLTCTTSGFTFSSYGMHWIRQAPGKGLEWIAYISSGGGTIYYSQSFQGRFTISRDNSRNQLYLQMNSLKTEDSAVYYCARHPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJD3</accession>
    <name>A0A672YJD3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zgc:153867</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">zgc:153867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJD3"/>
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        <property type="protein sequence ID" value="ENSSORP00005004668.1"/>
        <property type="gene ID" value="ENSSORG00005001985.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJD3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000019">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF7">
        <property type="entry name" value="SIMILAR TO MYOSIN, LIGHT POLYPEPTIDE 6, ALKALI, SMOOTH MUSCLE AND NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="202" mass="22129" checksum="43D926E57B113955" modified="2020-06-17" version="1">MAPKREASIKKSPSPVAQPRLGPPFPCLPATPQSPKPAPELKPRMLRPSDSPFDPSTLEVSELKEAFLLFDKTGEGKISYSQAGDVMRALGQNPVNAEVLKVLGNPKAEDMNSKMLDFEQFLPMFQAIAKNKDQGSMEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLAGHEDANGSINYEAFVRHIMSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLK9</accession>
    <name>A0A672YLK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLK9"/>
    <dbReference type="Ensembl" id="ENSSORT00005005650.1">
        <property type="protein sequence ID" value="ENSSORP00005005464.1"/>
        <property type="gene ID" value="ENSSORG00005003273.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLK9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF287">
        <property type="entry name" value="CADHERIN-RELATED NEURONAL RECEPTOR VARIABLE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025363211" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="27"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="688"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="131"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="240"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="348"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="453"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="576"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="786" mass="86033" checksum="255D9A28D8AAA5F2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLU9</accession>
    <name>A0A672YLU9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plexin-B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">plxnb1b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for SEMA4D. Plays a role in GABAergic synapse development. Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A672YLU9">
        <property type="interactions" value="709"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005817.1">
        <property type="protein sequence ID" value="ENSSORP00005005575.1"/>
        <property type="gene ID" value="ENSSORG00005003276.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLU9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12793">
        <property type="entry name" value="RasGAP_plexin_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Plexin B1"/>
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    </dbReference>
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        <property type="entry name" value="Plexin b1a"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
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    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_E-set"/>
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    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
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    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
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    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
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        <property type="entry name" value="Plexin_RBD"/>
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    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
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        <property type="entry name" value="PSI"/>
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    <dbReference type="InterPro" id="IPR057533">
        <property type="entry name" value="PSI_Plexin-B"/>
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    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
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    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
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    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
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        <property type="entry name" value="TIG1_plexin"/>
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    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
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    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLEXIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PSI"/>
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    <dbReference type="Pfam" id="PF24479">
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
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    </dbReference>
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        <property type="entry name" value="Plexin repeat"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SEMA"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
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        <location>
            <begin position="39"/>
            <end position="2166"/>
        </location>
    </feature>
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        <location>
            <begin position="1525"/>
            <end position="1545"/>
        </location>
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            <begin position="24"/>
            <end position="492"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="790"/>
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            <begin position="1325"/>
            <end position="1347"/>
        </location>
    </feature>
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        <location>
            <begin position="1471"/>
            <end position="1491"/>
        </location>
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            <begin position="696"/>
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            <begin position="728"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
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            <begin position="790"/>
            <end position="807"/>
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            <begin position="1329"/>
            <end position="1339"/>
        </location>
    </feature>
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        <location>
            <begin position="1471"/>
            <end position="1480"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00057668"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070678"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2166" mass="239627" checksum="751BB93E79630C22" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YM54</accession>
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        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase subunit NDUFA4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ndufa4a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the complex IV NDUFA4 subunit family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A672YM54"/>
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        <property type="term" value="C:mitochondrial inner membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
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    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="82" mass="9519" checksum="44DD6A12D982215C" modified="2020-06-17" version="1">MLALVRKQLRNHPALIPLFIFIGGGATMSMVYLARLGLRNPDVCWDRKNNPEPWNKMGPTDQYKFYTVNMDYSKLKKEGPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YM75</accession>
    <name>A0A672YM75_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">slc4a1a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72363"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YM75"/>
    <dbReference type="FunCoup" id="A0A672YM75">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005917.1">
        <property type="protein sequence ID" value="ENSSORP00005005674.1"/>
        <property type="gene ID" value="ENSSORG00005003443.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YM75"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0015106">
        <property type="term" value="F:bicarbonate transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000020">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001717">
        <property type="entry name" value="Anion_exchange"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018241">
        <property type="entry name" value="Anion_exchange_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF12">
        <property type="entry name" value="BAND 3 ANION TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00165">
        <property type="entry name" value="ANIONEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00220">
        <property type="entry name" value="ANION_EXCHANGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="470"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="574"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="662"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="704"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="760"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="786"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="830"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="856"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="13">
        <location>
            <begin position="69"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="13">
        <location>
            <begin position="197"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="12">
        <location>
            <begin position="357"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="909" mass="102002" checksum="1E763A4DD0B078D4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZM5</accession>
    <name>A0A672YZM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Septin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">septin7b</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
            <location evidence="5">Centromere</location>
            <location evidence="5">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19 20">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZM5"/>
    <dbReference type="Ensembl" id="ENSSORT00005010318.1">
        <property type="protein sequence ID" value="ENSSORP00005009988.1"/>
        <property type="gene ID" value="ENSSORG00005001124.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZM5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000162">
        <property type="entry name" value="septin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008115">
        <property type="entry name" value="Septin7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01742">
        <property type="entry name" value="SEPTIN7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="12 21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14 20" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="22">
        <location>
            <begin position="37"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="334"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="436" mass="50643" checksum="BFEB7C881DDD2772" modified="2020-06-17" version="1">METGGFIVKSLVQQKNLEGYVGFASLPNQVYRKSVKRGFEFTLMVVGESGLGKSTLINSLFLTDLYSAEYPGPSHRIKKTVQVEQSKVLVKEGGVQLLLTIVDTPGFGDAVDNSNCWQPIIDHIDSKFEDYLNSESRVNRRQMPDSRIHCCLYFIAPSGHGLKPLDIEFMKRLHEKVNVIPLIAKADTLTPEECQQFKKQIMREVQEHKIKIYEFPETDDEEENRLVKKIKDKLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENSDHCDFTILRDMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNRAKGQLSTKLDTVEGMSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRVFEEERSNWEAQQRLEQQKLEASRTLEKNKKKGKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z092</accession>
    <name>A0A672Z092_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z092"/>
    <dbReference type="Ensembl" id="ENSSORT00005010833.1">
        <property type="protein sequence ID" value="ENSSORP00005010496.1"/>
        <property type="gene ID" value="ENSSORG00005005669.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z092"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000203">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF52">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY POLYPEPTIDE B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025713544" description="UDP-glucuronosyltransferase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="525" mass="60447" checksum="71FB8B78C596E5EA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0D9</accession>
    <name>A0A672Z0D9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Diencephalon/mesencephalon homeobox 1a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dmbx1a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z0D9"/>
    <dbReference type="FunCoup" id="A0A672Z0D9">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010417.1">
        <property type="protein sequence ID" value="ENSSORP00005010087.1"/>
        <property type="gene ID" value="ENSSORG00005005486.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0D9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061074">
        <property type="term" value="P:regulation of neural retina development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000125">
        <property type="entry name" value="diencephalon/mesencephalon homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052488">
        <property type="entry name" value="DMBX_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46639">
        <property type="entry name" value="DIENCEPHALON/MESENCEPHALON HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46639:SF3">
        <property type="entry name" value="DIENCEPHALON_MESENCEPHALON HOMEOBOX PROTEIN 1-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="64"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="367"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="66"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="124"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="193"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="42705" checksum="85D361AE67C9F56E" modified="2020-06-17" version="1">MQHYGVNGYSLHAMNSLSAMYNLHQQAAQQAQHAPDYRPSVHALTLAERLADIILEARYGSQHRKQRRSRTAFTAQQLEALEKTFQKTHYPDVVMRERLAMCTNLPEARVQVWFKNRRAKFRKKQRSLQKEQLQKQKEASGEGGEKEDAPPSTTVLPDTQLPPSSSSSSSSSLEAEGPVVRPVPLDMELNVTSAEHSGSESATEDNATDKEDENKSQRGEEMKCERAATPGEVSSACKRLSPKPDSPLVSPSVTPSSSSSGLASSGPLSQSHSYSSSPLSLFRLQEQFRQHMAATNNLVHYPAFDLAAPSSLPYLGMNVNMPPAPLGSLPCQSYYQSLSHHAQQVWNSPLLQASAAGGLSSHNSKTTSIENLRLRAKQHAASLGLDTMSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1P5</accession>
    <name>A0A672Z1P5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Periplakin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ppl</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer or a heterodimer with EVPL. Found in a complex composed of PPL (via C-terminal linker domain), BFSP1 and BFSP2 in the retinal lens. Within the complex interacts (via C-terminal linker domain) with BFSP2. Interacts with VIM. Binds to the PH domain of AKT1. Interacts with FCGR1A. May interact with PPHLN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the plakin or cytolinker family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029998269.1">
        <property type="nucleotide sequence ID" value="XM_030142409.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A672Z1P5">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011015.1">
        <property type="protein sequence ID" value="ENSSORP00005010673.1"/>
        <property type="gene ID" value="ENSSORG00005002449.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424984"/>
    <dbReference type="CTD" id="5493"/>
    <dbReference type="InParanoid" id="A0A672Z1P5"/>
    <dbReference type="OrthoDB" id="29745at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000109">
        <property type="entry name" value="Periplakin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000143">
        <property type="entry name" value="Periplakin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.780:FF:000001">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000030">
        <property type="entry name" value="Short stop, isoform K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058847">
        <property type="entry name" value="Plectin_PPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055419">
        <property type="entry name" value="Spectrin_PEPL/EVPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF10">
        <property type="entry name" value="PERIPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26346">
        <property type="entry name" value="Plectin_PPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23160">
        <property type="entry name" value="Spectrin_1st_PEPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21097">
        <property type="entry name" value="SR_plectin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Periplakin/Envoplakin N-terminal" evidence="15">
        <location>
            <begin position="30"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Desmoplakin SH3" evidence="14">
        <location>
            <begin position="386"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="16">
        <location>
            <begin position="1005"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="16">
        <location>
            <begin position="1241"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="612"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="775"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="898"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="963"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1069"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1247"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1374"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1509"/>
            <end position="1625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF26346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1757" mass="207121" checksum="C1E26A6EE84D2F28" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3N8</accession>
    <name>A0A672Z3N8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z3N8"/>
    <dbReference type="Ensembl" id="ENSSORT00005011745.1">
        <property type="protein sequence ID" value="ENSSORP00005011364.1"/>
        <property type="gene ID" value="ENSSORG00005006091.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3N8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="9"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="112" mass="12457" checksum="BECC3C01F7A7E3F9" modified="2020-06-17" version="1">MRTVCDTRPTLTVLPPSTEELQQGKATLMCLANKGFPSDWSLSWKVNGGSSISWEESRSPAVLQKDGHYSWSSTLRLPADQWRKVNSVTCEATQGSQSALSHTLRTHQCSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3P1</accession>
    <name>A0A672Z3P1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="18">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">b4galt6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17950"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:31496"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Golgi apparatus membrane</location>
            <topology evidence="18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z3P1"/>
    <dbReference type="Ensembl" id="ENSSORT00005011801.1">
        <property type="protein sequence ID" value="ENSSORP00005011420.1"/>
        <property type="gene ID" value="ENSSORG00005006109.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z3P1"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008489">
        <property type="term" value="F:UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006688">
        <property type="term" value="P:glycosphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000037">
        <property type="entry name" value="Beta-1,4-galactosyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF47">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="109"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="248"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="383" mass="44795" checksum="6A5EB1FC395EBDA3" modified="2020-06-17" version="1">ARGLMKRLLRMSNRSFLAFIFFFSMSTTCLYFIYVAPGIANTYFFMVQAQGIMLRDNVRTIGQMIRLYTNKNSTLNGTDYPDGTNSSEYLVQPTTYLPENFSYAQNLPCPERLPSMKGLMEVNMTEIPMEEIELKFSKIFDIEFGGHWKPKDCRPRWKVAILIPFRNRHEHLPILFQHLIPMLQRQRLQFAFYVIEQSGSQPFNRAMLFNVGFLEAMKDLDWDCLIFHDVDHIPENDRNYYGCGQMPRHFAAKLDKYMYILPYSEFFGGVSGLTVDQFRKINGFPNAFWGWGGEDDDLWNRVHYAGLNVTRPEGEIGKYKSIPHHHRGEVQFLGRYKLLRYSKERQHLDGLNNLHYSPLVSLSSLYKNITVDLHPELAPIADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4R8</accession>
    <name>A0A672Z4R8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal fusion of the chorion and the allantois during placenta development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z4R8"/>
    <dbReference type="FunCoup" id="A0A672Z4R8">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011838.1">
        <property type="protein sequence ID" value="ENSSORP00005011457.1"/>
        <property type="gene ID" value="ENSSORG00005006128.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z4R8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19339">
        <property type="entry name" value="Wnt_Wnt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013300">
        <property type="entry name" value="Wnt7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF73">
        <property type="entry name" value="PROTEIN WNT-7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01891">
        <property type="entry name" value="WNT7PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025593963" description="Protein Wnt" evidence="12">
        <location>
            <begin position="21"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="336" mass="37666" checksum="68481B63EF1B676A" modified="2020-06-17" version="1">MCCGFFPPFGLALSSVVALGANIICNKIPGLAPRQRALCQSRPDAIIVIGEGAQLGINECQYQFRYGRWNCSALGERTVFGQELRVGSREAAFTYAITAAGVAHAVTAACSQGNLSQCGCDREKQGYHDQEEGWKWGGCSADVKYGVEFSRRFVDAREIKKNARRLMNLHNNEAGRKILEERMKLECKCHGVSGSCTTKTCWITLPKFREIGYLLKERYSEAVQVEPVRASRLRQPSFLRLKLARGYQKPTDTDLVFLERSPNYCEEDTSTGSTGTRGRLCNGTSTYTDGCNVMCCGRGYNTHHYTRVWQCNCKFHWCCFVKCNTCSEKSEVFTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5Q1</accession>
    <name>A0A672Z5Q1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">si:dkey-204f11.64</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z5Q1"/>
    <dbReference type="FunCoup" id="A0A672Z5Q1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013033.1">
        <property type="protein sequence ID" value="ENSSORP00005012636.1"/>
        <property type="gene ID" value="ENSSORG00005006612.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z5Q1"/>
    <dbReference type="OrthoDB" id="6264244at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="11">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="71" mass="7632" checksum="2A9C77C69A1036B1" modified="2020-06-17" version="1">MSNSGANSSLVLAQKAVKQLRLEASVRRIKVSQAAAELKTFCLQNAHKDPLLTGVPSSDNPFRPPKSCVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z651</accession>
    <name>A0A672Z651_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 178B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tmem276b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM178 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z651"/>
    <dbReference type="FunCoup" id="A0A672Z651">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012722.1">
        <property type="protein sequence ID" value="ENSSORP00005012325.1"/>
        <property type="gene ID" value="ENSSORG00005006502.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z651"/>
    <dbReference type="OrthoDB" id="8809386at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000007">
        <property type="entry name" value="transmembrane protein 178B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039625">
        <property type="entry name" value="T178A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005:SF3">
        <property type="entry name" value="SI:CH211-150G13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="298" mass="33538" checksum="C6AF70FDC69C2FCC" modified="2020-06-17" version="1">MAAMKILTGTGLFLAFCALGLLAMAICTDYWYETDARRHRERCKNYANKRNDPGYIYISNHNLPLQMPPRRVERKGNGPDVSALIRVKRHFLAAASAMESHCSRQFNSTISGLWRKCHREGFDLETEDLIYKGIIQRCTPVKYHYSSSILPRNLPINITKTIRQDEWHALHLRRMTAGFVGMAVSIILFGWIIGVLGCCQQHDLMQYVAGLLFLMGGTCCIISLCTCVAGINFELSRYPRYMYGLPEDISHGYGWSMFCAWGGLGLTLLAGFLCTLAPSLSTPARTTTHKPRQENGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7J2</accession>
    <name>A0A672Z7J2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proline-rich transmembrane protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">prrt1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030003291.1">
        <property type="nucleotide sequence ID" value="XM_030147431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z7J2"/>
    <dbReference type="FunCoup" id="A0A672Z7J2">
        <property type="interactions" value="882"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013751.1">
        <property type="protein sequence ID" value="ENSSORP00005013341.1"/>
        <property type="gene ID" value="ENSSORG00005006917.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428398"/>
    <dbReference type="CTD" id="80863"/>
    <dbReference type="InParanoid" id="A0A672Z7J2"/>
    <dbReference type="OrthoDB" id="5989578at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051423">
        <property type="entry name" value="CD225/Dispanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948">
        <property type="entry name" value="NG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948:SF21">
        <property type="entry name" value="PROLINE-RICH TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="25673" checksum="A1C9DBF5B9DF2FD3" modified="2020-06-17" version="1">MSSEKHGLDDSGRQTMQPPPYLPGPQDPNAPPHPNIPPAPGTQPNYPPPPPPPGSEGYLQETQFHCTPLGPPGAPQGYTVQTQGPGGTIPHPPVGYLHPGYPLQLQPCTAYVPVYPMASGQPYMPGGAGHPGIPPGQIHPMQMPPSITLMDRRPPHDYLPIAVLTTVCCFWPTGIIAIIKAVQVRTAIARGDMVTAEIASREARNFSFISLAVGIASIVLCTILTVVVIIASQHHDDDWEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z883</accession>
    <name>A0A672Z883_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Thyrotroph embryonic factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tefb</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. PAR subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z883"/>
    <dbReference type="Ensembl" id="ENSSORT00005013477.1">
        <property type="protein sequence ID" value="ENSSORP00005013069.1"/>
        <property type="gene ID" value="ENSSORG00005006809.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z883"/>
    <dbReference type="OrthoDB" id="6022300at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000007">
        <property type="entry name" value="hepatic leukemia factor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF47">
        <property type="entry name" value="TEF TRANSCRIPTION FACTOR, PAR BZIP FAMILY MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="287"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="160"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="319"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="357" mass="38906" checksum="ADD2309839CA43AF" modified="2020-06-17" version="1">MPGKAAVTVAIKPGVNTVTTPQKSFPFVLKKIMDIPPPNILEEGDDEIEKEKLCSSEDAEGGGDGVASAGGGPRGSGGGGGVSASLTPAIWDKTIPYDGETFHLEYMDLDEFLLENGIPVTLEEEELQKTLSSVEGKGKCIPKVIATITTGAAVSASASASAASPLASTTSTVNSDPEEPVTVTTLQPAKIEEEEEEEEQEEETEEAVQEVNVKEKKTDRNTPSPIDPDAIEVDINFQPDPTDLVLSSVPGGELFNPRKHKFSEEELKPQPMIKKAKKVFVPDEQKDDKYWSRRKKNNLAAKRSRDARRLKENQITVRASFLERENAALRQQVAELRKDCGRCKNILARYEAKYGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8V3</accession>
    <name>A0A672Z8V3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z8V3"/>
    <dbReference type="Ensembl" id="ENSSORT00005013683.1">
        <property type="protein sequence ID" value="ENSSORP00005013275.1"/>
        <property type="gene ID" value="ENSSORG00005006876.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z8V3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF391">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="124"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="167"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="264"/>
        </location>
    </feature>
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        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="45"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="321" mass="36304" checksum="F2F065C0BCDD8C67" modified="2020-06-17" version="1">MMVNSTHFSHFTLSVFILDGFDELGPLRPVLVIPYLVMFVLSLFANFLLLYAITSQRSLHSPMYILIAVLSGVDVSLPLFFVPHMLLNLALGWRGISLTGCLIQMFIIHLLGTFQSTLLLWMSLDRYFAICTPLHYHEYMALKKFIKFVVPLVIRNVFMIMLIVTLVRKLWFCGGNVINHCFCEHMAVVALACGSTAVNNLAGLLTVFFIPVADFILITVSYVIIFSSVLRSGRSGVKALHTCVTHIVVITVSLMIILVAFLSYRVQNRLPAVVRVFFSTMYLFFLSCFNPIIYGIRTTEIRQIFLKTLSCCRPVRTLPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z948</accession>
    <name>A0A672Z948_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeats and calponin homology (CH) domain containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lrch3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z948"/>
    <dbReference type="Ensembl" id="ENSSORT00005013779.1">
        <property type="protein sequence ID" value="ENSSORP00005013369.1"/>
        <property type="gene ID" value="ENSSORG00005006909.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z948"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000021">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000007">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF44">
        <property type="entry name" value="LEUCINE RICH REPEATS AND CALPONIN HOMOLOGY DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="589"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="706"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="707"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="727" mass="79647" checksum="116ECF876B551F29" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9E5</accession>
    <name>A0A672Z9E5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z9E5"/>
    <dbReference type="Ensembl" id="ENSSORT00005013561.1">
        <property type="protein sequence ID" value="ENSSORP00005013153.1"/>
        <property type="gene ID" value="ENSSORG00005006824.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9E5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001155">
        <property type="entry name" value="Zinc finger 30C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="380" mass="43291" checksum="C92A12F3AD471AB2" modified="2020-06-17" version="1">DQVDPEPPHTKEEENLEYDEDKAQSSQLHQTQTEENTEEPEDGDDYGESEPDSIQKNDFPISDVACKTTQHQCTKDDKKYKLLSVSETQMTVDTTKKCFCCSVCNRGFVHKSKLKRHMSVHTGEKPFSCSVCKRGFARQFEFKQHMLLHTGEKPFCCSVCKKSFANKSNLKNHMSVHTGEKPFCCSVCKKSFARKRNLKEHMSVHTGEKQRPFSCSVCKKGFARKSLVKEHMSVHTGEKPFSCSICKKCFAHISNLKNHMIVHTVERPFSCSVCKKSFSRKSDVKHHMPVHTKEKPFSCSVCKKCFAHTSNLKNHMLVHTVERPFSCSVCKKGFALKGNLKKHMLVHTGEKPFCCSVCKKCFAHASNLKNHMLVHTVESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZB31</accession>
    <name>A0A672ZB31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tars1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZB31"/>
    <dbReference type="Ensembl" id="ENSSORT00005014946.1">
        <property type="protein sequence ID" value="ENSSORP00005014521.1"/>
        <property type="gene ID" value="ENSSORG00005006676.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZB31"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF54">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="14">
        <location>
            <begin position="68"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="340"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="713" mass="82357" checksum="A59165BA17A71B1D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDD9</accession>
    <name>A0A672ZDD9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Lamin-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115428924</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus lamina</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDD9"/>
    <dbReference type="Ensembl" id="ENSSORT00005015293.1">
        <property type="protein sequence ID" value="ENSSORP00005014864.1"/>
        <property type="gene ID" value="ENSSORG00005007558.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDD9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005652">
        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090435">
        <property type="term" value="P:protein localization to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000033">
        <property type="entry name" value="Lamin A/C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721">
        <property type="entry name" value="LAMIN DM0-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721:SF5">
        <property type="entry name" value="PRELAMIN-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="15">
        <location>
            <begin position="33"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="14">
        <location>
            <begin position="445"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="386"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="37"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="333"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="648" mass="72901" checksum="984A8D08C7DEF065" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZG61</accession>
    <name>A0A672ZG61_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZG61"/>
    <dbReference type="Ensembl" id="ENSSORT00005016515.1">
        <property type="protein sequence ID" value="ENSSORP00005016015.1"/>
        <property type="gene ID" value="ENSSORG00005008091.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZG61"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3590.10">
        <property type="entry name" value="acid-sensing ion channel 1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF170">
        <property type="entry name" value="ACID-SENSING ION CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 12" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="10 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="203" mass="23333" checksum="E3A822FC8AFE4735" modified="2025-10-08" version="2">MELSFSEESIESVRPSNLQAFANISTLHGMSHIFAYGHMTFRRFLWTLSFLGSLGLLMLVCMDRVSYYLEYPHVTKLDEVAAPNLTFPAVTFCNLNEFRFSKITRNDLYHVGELLALLNNNYQIANPHLAEPEVLAALKDKANFQNFKPKLFNMTDFYNRTGHDIGDMLLQCTFRGEECYPVNFSTVSSHSTHDSLSFSFIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHQ3</accession>
    <name>A0A672ZHQ3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IQ motif and Sec7 domain ArfGEF 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">iqsec2b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRAG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZHQ3"/>
    <dbReference type="Ensembl" id="ENSSORT00005016791.1">
        <property type="protein sequence ID" value="ENSSORP00005016284.1"/>
        <property type="gene ID" value="ENSSORG00005008147.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZHQ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13318">
        <property type="entry name" value="PH_IQSEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000001">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000001">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000004">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033742">
        <property type="entry name" value="IQSEC_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF314">
        <property type="entry name" value="IQ MOTIF AND SEC7 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16453">
        <property type="entry name" value="IQ_SEC7_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="476"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="333"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="884"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="403"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="898"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="912" mass="101521" checksum="E67AF97BB9C37780" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHX9</accession>
    <name>A0A672ZHX9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NIPSNAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nipsnap2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Protein involved in mitophagy. Accumulates on the mitochondria surface in response to mitochondrial depolarization and acts as a 'eat me' signal by recruiting proteins involved in selective autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NipSnap family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZHX9"/>
    <dbReference type="FunCoup" id="A0A672ZHX9">
        <property type="interactions" value="1774"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016585.1">
        <property type="protein sequence ID" value="ENSSORP00005016082.1"/>
        <property type="gene ID" value="ENSSORG00005007790.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZHX9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000003">
        <property type="entry name" value="Protein NipSnap homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000007">
        <property type="entry name" value="protein NipSnap homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF14">
        <property type="entry name" value="PROTEIN NIPSNAP HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="NIPSNAP" evidence="8">
        <location>
            <begin position="236"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="335" mass="39518" checksum="AC53290239B0B4E6" modified="2020-06-17" version="1">MKQYVALFSKAILFYSILFYSILFYSILFYSILFYSILSYLCLCFALPRMPSVSPWSCTATCWSEPCTAPLCCALARWKMATGVLQKVSSEDSWFKSLFVRKVDPRKDAHSNLLTKNEESNLYKIQFHNVKPECLDAYNKLCEEVLPSIHADKYYPCELVGTWNTWYGEQDQAVHLWRYRGGYPALTEVMNKLRQNKEFTAYRKERGKMLLSRRNQLLLEFSFWNEPVPREGPNIYELRSYQLRPGTMIEWGNYWARAIGFRQHDREAVGGFFSQIGNLYMVHHLWAYKDLQSRENTRNGAWQQEGWHEVVYYTVPLIQHMDSRIMIPTKASPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLG8</accession>
    <name>A0A672ZLG8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Far upstream element (FUSE) binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fubp3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZLG8"/>
    <dbReference type="Ensembl" id="ENSSORT00005017794.1">
        <property type="protein sequence ID" value="ENSSORP00005017278.1"/>
        <property type="gene ID" value="ENSSORG00005008512.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLG8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000060">
        <property type="entry name" value="Far upstream element binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000007">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="80"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="165"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="256"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="353"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="564" mass="61626" checksum="D807B104ECD56728" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZPE1</accession>
    <name>A0A672ZPE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative monooxygenase p33MONOX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">kiaa1191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the P33MONOX family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZPE1"/>
    <dbReference type="FunCoup" id="A0A672ZPE1">
        <property type="interactions" value="1283"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019238.1">
        <property type="protein sequence ID" value="ENSSORP00005018689.1"/>
        <property type="gene ID" value="ENSSORG00005009209.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZPE1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026759">
        <property type="entry name" value="P33MONOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28342">
        <property type="entry name" value="MONOOXYGENASE P33MONOX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28342:SF1">
        <property type="entry name" value="MONOOXYGENASE P33MONOX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15302">
        <property type="entry name" value="P33MONOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="334" mass="36181" checksum="494BB4C5BFAAC2FC" modified="2020-06-17" version="1">MSSRDEDIPAIESSTSSGIFGALSSPIGMIRRNISYDELIDAPMNSPPPDFSVNILWKDPVIPQRRFRNTEEEAESGGKLVTFEATSPAKSQVPVVKAKATSLMSSLMTKQTHENIQRFEHQAGLTDTGYSPHKGLSAEETRFSRLADSNLPKLKMPSGDFKEDRLTTSAQSTPGGTPSVTPSATPSVTPSVTPCVSPHSSPALHRRNWFQLSPTPFLTASEHNSLNPSTDMGGNEGGGGGERWNFFGTRSVVQKSPTDPGSDTGTGFSLQSYFGIQKSSTMDYTNTQINLKVEDPAQFLPPKIEITGIEAQRAPSRPHKLKPRDMNVLTPSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZV42</accession>
    <name>A0A672ZV42_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heme-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">soul3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZV42"/>
    <dbReference type="Ensembl" id="ENSSORT00005021663.1">
        <property type="protein sequence ID" value="ENSSORP00005021036.1"/>
        <property type="gene ID" value="ENSSORG00005010268.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZV42"/>
    <dbReference type="OrthoDB" id="9420729at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.80.10:FF:000007">
        <property type="entry name" value="Heme-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF7">
        <property type="entry name" value="SOUL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    <evidence type="ECO:0000313" key="4">
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        </source>
    </evidence>
    <sequence length="277" mass="31115" checksum="EF58D02B72DB251F" modified="2020-06-17" version="1">MDRGGCQMNGGGGGGGGGGGPDRHGMITLEDLESFSEDQLSDSGNGSLEEEGETMGEEEQDRLLHYWQDVARGHQVEVSEDMAEPIQQLTSNNQGRSNREHIPFTLLSRKDKCGEVLYEKRHYEKGHWACITMREDTYEQSICYGFMRIMRYICEQNSLGDYLGMTLPIVTVVHTDETHSVISNDVTVAYYLPLDHQAQPPQPYDSEITIEVWPAAVVYTRAFTGPTNEVTIINQINTMAELLDSPGVCINDSFIVAGYTNPAHSNRQNEIWFLERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWY1</accession>
    <name>A0A672ZWY1_9TELE</name>
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        <submittedName>
            <fullName evidence="17">Bromodomain and PHD finger containing, 3a</fullName>
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        <name evidence="17" type="primary">brpf3a</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005021790.1"/>
        <property type="gene ID" value="ENSSORG00005010640.1"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
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        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="BROMODOMAIN AND PHD FINGER-CONTAINING PROTEIN 3 ISOFORM X1"/>
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        <property type="entry name" value="PHD FINGER PROTEINS"/>
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        <property type="entry name" value="zf-HC5HC2H_2"/>
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        <property type="entry name" value="BROMODOMAIN"/>
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        <property type="entry name" value="BROMO"/>
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        <property type="match status" value="2"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
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            <begin position="248"/>
            <end position="298"/>
        </location>
    </feature>
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            <begin position="15"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1223" mass="137066" checksum="BE3C86729EB61F41" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZX71</accession>
    <name>A0A672ZX71_9TELE</name>
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        <submittedName>
            <fullName evidence="6">Transmembrane protein 234</fullName>
        </submittedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the TMEM234 family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF10639">
        <property type="entry name" value="TMEM234"/>
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        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="135" mass="14776" checksum="3D3B8D8312E82B44" modified="2025-10-08" version="2">MSLLLVSVLWGCTNPFLKRGTEGIEHVEKTNRVSQLLAEVKFLFLNLKYLVPFLLNQSGSLVYYYTLSTTELSLAVPVANSLTFLCTLLTGKLLGEEFGGKQAVAGMFLTMAGITLCVMSSMDDSVKQNVTEAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A069</accession>
    <name>A0A673A069_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">psma2a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A069"/>
    <dbReference type="FunCoup" id="A0A673A069">
        <property type="interactions" value="1706"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023366.1">
        <property type="protein sequence ID" value="ENSSORP00005022701.1"/>
        <property type="gene ID" value="ENSSORG00005011031.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A069"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000012">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="234" mass="25842" checksum="4073620C608E5629" modified="2020-06-17" version="1">MADRGYSFSLTTFSPSGKLVQIEYALAAVAAGAPSVGIKASNGVVLATEKKQKSILYDEQSVHKVEPITKHIGMVYSGMGPDYRVLVRRARKLAQQYFLVYQEPIPTGQLVQRVASVMQEYTQSGGVRPFGVSLLIAGWDEDHPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNNDLELEDAIHTAILTLKESFEGQMTEENIEVGICNEAGFKRLTPAEVKDYLAAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0W0</accession>
    <name>A0A673A0W0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein smoothened</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">smo</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004951.1">
        <property type="nucleotide sequence ID" value="XM_030149091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A0W0"/>
    <dbReference type="FunCoup" id="A0A673A0W0">
        <property type="interactions" value="1219"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023602.1">
        <property type="protein sequence ID" value="ENSSORP00005022931.1"/>
        <property type="gene ID" value="ENSSORG00005011097.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429540"/>
    <dbReference type="CTD" id="6608"/>
    <dbReference type="InParanoid" id="A0A673A0W0"/>
    <dbReference type="OrthoDB" id="10064659at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005113">
        <property type="term" value="F:patched binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071679">
        <property type="term" value="P:commissural neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15030">
        <property type="entry name" value="7tmF_SMO_homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07451">
        <property type="entry name" value="CRD_SMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000010">
        <property type="entry name" value="Smoothened, frizzled class receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000068">
        <property type="entry name" value="Smoothened, frizzled class receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035683">
        <property type="entry name" value="SMO_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041771">
        <property type="entry name" value="SMO_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF35">
        <property type="entry name" value="PROTEIN SMOOTHENED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025586827" description="Protein smoothened" evidence="20">
        <location>
            <begin position="29"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="21">
        <location>
            <begin position="42"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="22">
        <location>
            <begin position="205"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="756"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="821" mass="92571" checksum="9B389B3AFCFD20DA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2V4</accession>
    <name>A0A673A2V4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Hexosyltransferase</fullName>
            <ecNumber evidence="17">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">b3galt6</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. Has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. Has no activity towards substrates with terminal glucosamine or galactosamine residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3-O-(beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-galactose = 3-O-(beta-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11780"/>
            <dbReference type="Rhea" id="RHEA-COMP:12570"/>
            <dbReference type="Rhea" id="RHEA-COMP:12571"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:132088"/>
            <dbReference type="ChEBI" id="CHEBI:132090"/>
            <dbReference type="EC" id="2.4.1.134"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:11781"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; chondroitin sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Golgi apparatus membrane</location>
            <topology evidence="2 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_029989405.1">
        <property type="nucleotide sequence ID" value="XM_030133545.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029989406.1">
        <property type="nucleotide sequence ID" value="XM_030133546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A2V4"/>
    <dbReference type="FunCoup" id="A0A673A2V4">
        <property type="interactions" value="395"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024530.1">
        <property type="protein sequence ID" value="ENSSORP00005023830.1"/>
        <property type="gene ID" value="ENSSORG00005011516.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418977"/>
    <dbReference type="CTD" id="126792"/>
    <dbReference type="InParanoid" id="A0A673A2V4"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047220">
        <property type="term" value="F:galactosylxylosylprotein 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000018">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF3">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="339" mass="38880" checksum="E1B884216F458E85" modified="2020-06-17" version="1">MNLIRLVCRHKTALVIGIVCSFAVVLVFLAKCTSETLKQGHADPPGFAPRASSLQLHPDQQNPPSTSKDLSAFLVVLITTGPKYTERRSIIRSTWLVKRDSDVLAMFVVGTQGLSSEDLQNLNTEQGRHKDLLLLPELRDSYENLTLKLLHMYSWLDQNVEFKFVLKADDDTFARLDLIKEELKGKESNRLYWGFFSGRGRVKTAGKWRESSWELCDYYLPYALGGGYVLSADLVHYVHLNAGYFKTWQSEDVSLGAWLAPVDVRRTHDPRFDTEYKSRGCNNKYLVTHKQSLEDMLEKHQTLQRDGRLCKEEVKLRLSYVYDWSVPPSQCCQRKDGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2Z0</accession>
    <name>A0A673A2Z0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Calcium channel, voltage-dependent, alpha 2/delta subunit 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel subunit alpha-2/delta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A2Z0"/>
    <dbReference type="Ensembl" id="ENSSORT00005023693.1">
        <property type="protein sequence ID" value="ENSSORP00005023022.1"/>
        <property type="gene ID" value="ENSSORG00005011154.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A2Z0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000007">
        <property type="entry name" value="Calcium voltage-gated channel auxiliary subunit alpha2delta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF59">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="214"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="378" mass="43141" checksum="E9506E2DA85DCCBD" modified="2020-06-17" version="1">MGRQMSLSVAIIIMVSGGSKNNVLIAKYKDIEGVVKIEEVDGMELVKKFAEEMEEMLGRKMKSVKRLAEAAEEADFYHMYNETLEFDYYNSMLINTVDEDGNPVPLGGEFTLEENEHFNKLPVNTQQSNIQVPTNVYNRDPDILNGAYMSEALNDVFIDNFQKDPTLTWQYFGSSTGFFRLYPGIQWIPDENGVVTFDCRNRNWYIQAATSPKDVIIVVDVSGSMKGLRLTIAKHTINTILDTLGENDFVNIIAYSDYVRYVEPCFKGTLVQADLDNREHFKLLVEELHVKGEGKVKKAMKESFKVLNEATAMGQGSLCNQAIMLITDGAMEDFQDVFEEFNWPERRVWHTSTELKKWMCKHANSEAVKASQKHLHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A444</accession>
    <name>A0A673A444_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A444"/>
    <dbReference type="Ensembl" id="ENSSORT00005024973.1">
        <property type="protein sequence ID" value="ENSSORP00005024260.1"/>
        <property type="gene ID" value="ENSSORG00005011679.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A444"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="138"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="31109" checksum="325207D0A6632C42" modified="2020-06-17" version="1">MMTTRLTQLILPLLSHPQTGTLLRRRLTPIHPHSPTLRWASSSGQVRYSSQPGPTSSPPDLDLRHSGGLPRPDKTGNPPCPDAVSLPHSPAGPHSPAGSPTNREYHYSMSPRPLFEPSTLIVGDSIIRHIRFANSITRCFPGATAAHILDKLPDLLPSLPSSVKRIIVHVGCNDTARRQSELTKTDFKTLFSFLQSCGRSVFISGPIPTLSRSAERFSRLLSLNTWLQLTCRTYDFGFIDNFNLFWNRPSFYRADGVHPNKLGSSTLAGNIQYTLHSAPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A7L3</accession>
    <name>A0A673A7L3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Adhesion molecule with Ig like domain 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. AMIGO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A7L3"/>
    <dbReference type="Ensembl" id="ENSSORT00005025929.1">
        <property type="protein sequence ID" value="ENSSORP00005025174.1"/>
        <property type="gene ID" value="ENSSORG00005012111.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A7L3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031283">
        <property type="entry name" value="AMIGO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24368">
        <property type="entry name" value="AMPHOTERIN-INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24368:SF62">
        <property type="entry name" value="AMPHOTERIN-INDUCED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025660624" evidence="14">
        <location>
            <begin position="23"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="272"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="457" mass="51321" checksum="284CD36FF93621B3" modified="2020-06-17" version="1">MTFGMYSICFLVLLYLFHGSEEICPSMCLCVSDIVSCSSSGLTKLPQSLPSFSVTLDLSHNHFSWLGPGSFKKMSRLENLQMAHNQIRTLGYGVFHNASGLRHLDLSSNRLQVVEEHYFQGLWRLEELLLFNNKIAQVESGTLSGLSSLKKAYFSLNQITHFPFFSIRDHSHPFLTMLDLSSNRLNRLPWEDVKALPGLVQRGLYLHNNSLICDCSMYSVFWHWQLRNYDSMKDFTDEHTCTIYGDSHASIRFLLHNRFFHNCTVEKAVSEPVTVYLSAVSVSEGGKVHLDCETSLRGANLSFTWLSPSKGRITQASVNDTLINVFANGTLEIQGAKVNDSGLYVCTAVDIKQALNATREVNVTVVLPAQEAFNTGYTTLLGCAVTLILILMYLYLTPCRCSFCKQPKPAANPTAAYNPNALTSVFSPSMRDEHKIPTDKHVAFLEPMMTRPKQEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8B6</accession>
    <name>A0A673A8B6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Peroxisomal coenzyme A diphosphatase NUDT7</fullName>
            <ecNumber evidence="13">3.6.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">Nucleoside diphosphate-linked moiety X motif 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">nudt7</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate. Cleaves CoA, CoA esters and oxidized CoA with similar efficiencies. Preferentially hydrolyzes medium-chain acyl-CoAs and bile acid-CoAs. Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester. May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA. Exhibits decapping activity towards dpCoA-capped RNAs in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:63001"/>
            <dbReference type="ChEBI" id="CHEBI:132021"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:50041"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>CoA + H2O = (R)-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:64988"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="3.6.1.77"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64989"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 5'-end CoA-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + (R)-4'-phosphopantetheine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67592"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="Rhea" id="RHEA-COMP:17276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
            <dbReference type="ChEBI" id="CHEBI:172371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:67593"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>acetyl-CoA + H2O = S-acetyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:64992"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:156266"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:64993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>butanoyl-CoA + H2O = S-butanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:49976"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132011"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:49977"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>choloyl-CoA + H2O = S-choloyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57373"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132020"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:50037"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>decanoyl-CoA + H2O = decanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
            <dbReference type="ChEBI" id="CHEBI:132014"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:50021"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>dodecanoyl-CoA + H2O = S-dodecanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50024"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132015"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:50025"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>hexanoyl-CoA + H2O = hexanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:49980"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
            <dbReference type="ChEBI" id="CHEBI:132012"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:49981"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>malonyl-CoA + H2O = malonyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:172363"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:67469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>octanoyl-CoA + H2O = S-octanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132013"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:50017"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>propanoyl-CoA + H2O = propanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67464"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:172362"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:67465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>succinyl-CoA + H2O = succinyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67472"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:172364"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:67473"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tetradecanoyl-CoA + H2O = tetradecanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50028"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132017"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:50029"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Nudix hydrolase family. PCD1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.77" evidence="13"/>
    <dbReference type="RefSeq" id="XP_029992378.1">
        <property type="nucleotide sequence ID" value="XM_030136518.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029992379.1">
        <property type="nucleotide sequence ID" value="XM_030136519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A8B6"/>
    <dbReference type="FunCoup" id="A0A673A8B6">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026199.1">
        <property type="protein sequence ID" value="ENSSORP00005025439.1"/>
        <property type="gene ID" value="ENSSORG00005012244.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420904"/>
    <dbReference type="CTD" id="283927"/>
    <dbReference type="InParanoid" id="A0A673A8B6"/>
    <dbReference type="OrthoDB" id="206213at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015938">
        <property type="term" value="P:coenzyme A catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000049">
        <property type="entry name" value="Peroxisomal coenzyme A diphosphatase NUDT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF24">
        <property type="entry name" value="PEROXISOMAL COENZYME A DIPHOSPHATASE NUDT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="30" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="30" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="31">
        <location>
            <begin position="26"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00059426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00072984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00079598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="222" mass="24651" checksum="C7D74E9BB009AFC0" modified="2020-06-17" version="1">MHVKERTIGILKRFDIGNRFSYLPTMPKASVLIPLFVKKGELHTLMTLRSMQLRSGAGEVCFPGGKRDPVDKDDVDTALREAEEEIGLPPDQVQVVCRLFPVINKSGLLVTPVVGFIDETFCPCPNPAEVSAVFSVPLDFFTSEKDHFTANGITGTVGPLHSFYFMDSNSGSQYHIWGLTAVLAILVSALALKKKPEFDVGFDFENPLSFFEQVLQRRISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A9B7</accession>
    <name>A0A673A9B7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Fatty acid 2-hydroxylase</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A9B7"/>
    <dbReference type="Ensembl" id="ENSSORT00005026562.1">
        <property type="protein sequence ID" value="ENSSORP00005025794.1"/>
        <property type="gene ID" value="ENSSORG00005012398.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A9B7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="262" mass="29916" checksum="5456D2C47AFC94B9" modified="2020-06-17" version="1">THKRSLWTVAFIHVNTFMSFSCFSTKVLLCLCVQDLVDWRKPLAWQVGLLGEKYDAWVHQPVDRPIRLFGNPLLEAGTKTSWYWVPVVWLPIVFYLSWYCYTTLAKGNTRIVLTSDFSIPVHKYYCIHRFVFHMKPPAHNYYLITLHFLLHGQHHKSPFDGSRLVFPPGLASLVVGTFYVILQNLLPGVLGVSVFVGGLCGYVVYDMIHYYLHYGSPKRGSYMYGLKAYHVKPTLSTRGQVGTPTPQGGSTCVCDMCRVRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AD47</accession>
    <name>A0A673AD47_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">GLIS family zinc finger 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115418371</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AD47"/>
    <dbReference type="Ensembl" id="ENSSORT00005027986.1">
        <property type="protein sequence ID" value="ENSSORP00005027201.1"/>
        <property type="gene ID" value="ENSSORG00005012998.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AD47"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000036">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000048">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000453">
        <property type="entry name" value="GLIS family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN GLIS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="368"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="402"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="435"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="465"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="495"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="125"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="184"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="287"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="585"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="759" mass="81076" checksum="08EA3D74A9137F52" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADI7</accession>
    <name>A0A673ADI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Kin of IRRE-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Kin of irregular chiasm-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Nephrin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">kirrel1b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL1 glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ADI7"/>
    <dbReference type="Ensembl" id="ENSSORT00005027601.1">
        <property type="protein sequence ID" value="ENSSORP00005026818.1"/>
        <property type="gene ID" value="ENSSORG00005012624.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ADI7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05898">
        <property type="entry name" value="IgI_5_KIRREL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000232">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000094">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000103">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF14">
        <property type="entry name" value="KIN OF IRRE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025403659" description="Kin of IRRE-like protein 1" evidence="19">
        <location>
            <begin position="31"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="511"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="27"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="133"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="236"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="321"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="405"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="642"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="760"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="648"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="693"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="703"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="778"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="816" mass="88774" checksum="18A4D2F9FFA57559" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADL9</accession>
    <name>A0A673ADL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psma1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993103.1">
        <property type="nucleotide sequence ID" value="XM_030137243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ADL9"/>
    <dbReference type="FunCoup" id="A0A673ADL9">
        <property type="interactions" value="1867"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028449.1">
        <property type="protein sequence ID" value="ENSSORP00005027655.1"/>
        <property type="gene ID" value="ENSSORG00005013214.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421368"/>
    <dbReference type="CTD" id="5682"/>
    <dbReference type="InParanoid" id="A0A673ADL9"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03749">
        <property type="entry name" value="proteasome_alpha_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000025">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035144">
        <property type="entry name" value="Proteasome_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="232"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="263" mass="29419" checksum="6A674E2F31B5D242" modified="2020-06-17" version="1">MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSRTHAVLVALKRAQSELAAHQKKILHVDNHVGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPTSRLVSLVGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCKAMSIGARSQSARTYLERLMDKFNDCNLNDLVQHGLRALRETLPTEQDLTTKNVSIGIVGKDMEFTIYDDDDVAPFLEGLEERPQRKVAQPADEAAPADAPDEPMEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AFB3</accession>
    <name>A0A673AFB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="5 17">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="5 17"/>
    <dbReference type="AlphaFoldDB" id="A0A673AFB3"/>
    <dbReference type="Ensembl" id="ENSSORT00005028741.1">
        <property type="protein sequence ID" value="ENSSORP00005027941.1"/>
        <property type="gene ID" value="ENSSORG00005013379.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AFB3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13363">
        <property type="entry name" value="PH_PLC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000071">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000088">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000058">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000039">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039504">
        <property type="entry name" value="PLC-delta3_EF-hand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF153">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14788">
        <property type="entry name" value="EF-hand_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 17" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="13 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="38"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="21">
        <location>
            <begin position="505"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="20">
        <location>
            <begin position="605"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="458"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="741"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="757"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="768" mass="86793" checksum="A90751BF849E9821" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AFN6</accession>
    <name>A0A673AFN6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115438573</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AFN6"/>
    <dbReference type="Ensembl" id="ENSSORT00005029291.1">
        <property type="protein sequence ID" value="ENSSORP00005028480.1"/>
        <property type="gene ID" value="ENSSORG00005013634.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AFN6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="98"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="184" mass="19821" checksum="813EDE16EB98B069" modified="2020-06-17" version="1">MSLLSCTTTAQLIVSPSRSQFFDTQSVSLSCEDEGPGWTLRRNTSRQTRAQCGRQWGKMKGSTCRISVLVPLDSGVYWCESSEGQTRTSISLSVTGGPLVLHSPVLPVMEGQEVSLHCQTHPPSNRSADFYKDGVFVGAGPAGHMTIPQVSLAHQGVYRCDITGVGQSPPSWMSVSGQRSPVCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALZ9</accession>
    <name>A0A673ALZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Titin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ALZ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005031566.1">
        <property type="protein sequence ID" value="ENSSORP00005030705.1"/>
        <property type="gene ID" value="ENSSORG00005014640.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALZ9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
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    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048738">
        <property type="term" value="P:cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000034">
        <property type="entry name" value="Titin isoform A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000012">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340:SF13">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="45"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="119"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="220"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="322"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="430"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="529"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="74876" checksum="19904FC3A35E6C47" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AM73</accession>
    <name>A0A673AM73_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="25">NACHT, LRR and PYD domains-containing protein 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC115428765</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Inflammasome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NLRP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the NOD1-NOD2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030003838.1">
        <property type="nucleotide sequence ID" value="XM_030147978.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030345.1">
        <property type="protein sequence ID" value="ENSSORP00005029512.1"/>
        <property type="gene ID" value="ENSSORG00005014097.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428765"/>
    <dbReference type="InParanoid" id="A0A673AM73"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061702">
        <property type="term" value="C:canonical inflammasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025307">
        <property type="entry name" value="FIIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13553">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23679">
        <property type="entry name" value="UPA-FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51830">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-1271">Inflammasome</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1210">Necrosis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="NACHT" evidence="23">
        <location>
            <begin position="97"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="FIIND" evidence="24">
        <location>
            <begin position="1004"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="domain" description="CARD" evidence="22">
        <location>
            <begin position="1299"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="924"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="942"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1394" mass="157708" checksum="87033059FDD6A01D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANX3</accession>
    <name>A0A673ANX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">msc</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029978574.1">
        <property type="nucleotide sequence ID" value="XM_030122714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ANX3"/>
    <dbReference type="Ensembl" id="ENSSORT00005031131.1">
        <property type="protein sequence ID" value="ENSSORP00005030283.1"/>
        <property type="gene ID" value="ENSSORG00005014440.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410890"/>
    <dbReference type="CTD" id="9242"/>
    <dbReference type="InParanoid" id="A0A673ANX3"/>
    <dbReference type="OrthoDB" id="6233288at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF72">
        <property type="entry name" value="HLH54F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="63"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="161" mass="18335" checksum="5FBC6FDFD80FC6CD" modified="2020-06-17" version="1">MSTGSPVSDAEDYDTTATPLERTTRKSTCYKAHLYSDEELEDDGRIRQERKLSKAHQKDARQTQRNAANARERARMRVLSKAFSRLKTSLPWVPADTKLSKLDTLRLASSYISHLRQLLQDDRFENSFAHPVNLTWPFMMTGRSEDNPDISSTVRLCGATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQ37</accession>
    <name>A0A673AQ37_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell death activator CIDE-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cidea</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AQ37"/>
    <dbReference type="FunCoup" id="A0A673AQ37">
        <property type="interactions" value="1278"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031388.1">
        <property type="protein sequence ID" value="ENSSORP00005030532.1"/>
        <property type="gene ID" value="ENSSORG00005014566.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQ37"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003508">
        <property type="entry name" value="CIDE-N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12306">
        <property type="entry name" value="CELL DEATH ACTIVATOR CIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12306:SF8">
        <property type="entry name" value="LIPID TRANSFERASE CIDEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02017">
        <property type="entry name" value="CIDE-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00266">
        <property type="entry name" value="CAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51135">
        <property type="entry name" value="CIDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CIDE-N" evidence="3">
        <location>
            <begin position="37"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="211" mass="24015" checksum="72EAABFD5A103AB6" modified="2020-06-17" version="1">MALQSGVTYMRTFLPETLQRSLSSVHASVRHILPSAQPRCFKVCTQSRRRRRSLTASTRDELLEQAVKAFSPSSRVLTLVLEEDGTEVDSEDFFQSLPVSTALMVLEKSQDWTQTKVVPSFRQPKRSGVAKLTFDLYKMNPKDFLCCLSVRASLYDMYTLSYELRCNRMKHLLRCVLRCLTCVTRVSAQMLLYASSSLLQFTGDDDDGDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQG2</accession>
    <name>A0A673AQG2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Selenoprotein F</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenoprotein M/F family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AQG2"/>
    <dbReference type="FunCoup" id="A0A673AQG2">
        <property type="interactions" value="1058"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031518.1">
        <property type="protein sequence ID" value="ENSSORP00005030657.1"/>
        <property type="gene ID" value="ENSSORG00005014626.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQG2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051084">
        <property type="term" value="P:'de novo' post-translational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.50:FF:000001">
        <property type="entry name" value="15 kDa selenoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.50">
        <property type="entry name" value="Sep15/SelM thioredoxin-like domain, active-site redox motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038219">
        <property type="entry name" value="Sep15/SelM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039992">
        <property type="entry name" value="Sep15_SelM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014912">
        <property type="entry name" value="Sep15_SelM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077:SF6">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08806">
        <property type="entry name" value="Sep15_SelM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Selenoprotein F/M" evidence="7">
        <location>
            <begin position="74"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="133" mass="14878" checksum="B074B76CF83F4BF5" modified="2020-06-17" version="1">IMLDMICLLSAFGADLSSEACRELGFSSNLLCSSCDLLGEFSLTKLQPDCRQCCQQEAQMEGRKLYAGAILEVSFVRSDKPKMFKGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AST4</accession>
    <name>A0A673AST4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cryz</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AST4"/>
    <dbReference type="FunCoup" id="A0A673AST4">
        <property type="interactions" value="1342"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033633.1">
        <property type="protein sequence ID" value="ENSSORP00005032735.1"/>
        <property type="gene ID" value="ENSSORG00005015539.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AST4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08253">
        <property type="entry name" value="zeta_crystallin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000244">
        <property type="entry name" value="quinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000016">
        <property type="entry name" value="Quinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051603">
        <property type="entry name" value="Zinc-ADH_QOR/CCCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44154:SF1">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="18"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="330" mass="35222" checksum="A64250DFAEEB2727" modified="2020-06-17" version="1">MLSVAVRLMRAIRVTEFGAPSVLKLSPEVTVPKPGHKQVLIRVHACGVNPVETYIRSGTFYRKPPLPYTPGSDVAGVVEDVGDGVTTVKAGDRVFTLATESGGYAEYTVASDICVYKLHDDLDFTQGAAIGVPYFTAYRALVHKAHAKPGETVLIHGASGGVGIAACQLSRALGLRVLGTAGTPEGMKLILNNGAHLAFNHREEGYTDKIMEATEGRGVDVIVEMLSNVNLSKDLQMMNYGGRVLVIGCRGSIEINPRDTMAKESSVIGVMLFLATPEENKEAAALFYAGMEAGWMRPIVGARYPLDKAAQAHHDIIESPGATGKIVLTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVP9</accession>
    <name>A0A673AVP9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AVP9"/>
    <dbReference type="Ensembl" id="ENSSORT00005033296.1">
        <property type="protein sequence ID" value="ENSSORP00005032408.1"/>
        <property type="gene ID" value="ENSSORG00005015367.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AVP9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="111" mass="13027" checksum="294A9DF97287B7E2" modified="2020-06-17" version="1">MNIPQNFIVAVLGVIPVGLGKKADKYTLNILFTAALKSITIRWMKPEPPSYTTWVQKVWDIYQMEQITFLLKLQKTTFTKRWEPMLPIYKCNVCEKKKDLYVLIQVFNLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AX77</accession>
    <name>A0A673AX77_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Matrin 3-like 1.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">matr3l1.2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AX77"/>
    <dbReference type="Ensembl" id="ENSSORT00005035255.1">
        <property type="protein sequence ID" value="ENSSORP00005034336.1"/>
        <property type="gene ID" value="ENSSORG00005016235.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AX77"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12436">
        <property type="entry name" value="RRM1_2_MATR3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="446"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="712"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="926"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="121"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="525"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="791"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="564"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="797"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="847"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="869"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="993" mass="110638" checksum="3B5F2A4A99FE97FF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZB8</accession>
    <name>A0A673AZB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 18">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="12">IMP dehydrogenase</shortName>
            <shortName evidence="12">IMPD</shortName>
            <shortName evidence="12">IMPDH</shortName>
            <ecNumber evidence="12 18">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">impdh1b</name>
        <name evidence="12" type="synonym">IMPDH</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12 18">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10 12 18">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 17">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="12 18"/>
    <dbReference type="AlphaFoldDB" id="A0A673AZB8"/>
    <dbReference type="FunCoup" id="A0A673AZB8">
        <property type="interactions" value="605"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036161.1">
        <property type="protein sequence ID" value="ENSSORP00005035226.1"/>
        <property type="gene ID" value="ENSSORG00005016626.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZB8"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000007">
        <property type="entry name" value="Chromosome 19 SCAF14664, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF129">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0129">CBS domain</keyword>
    <keyword evidence="1 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12 18" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 12 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12 14" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 12 15" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 12 18" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="19">
        <location>
            <begin position="188"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="19">
        <location>
            <begin position="253"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="12 13">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12 13">
        <location>
            <position position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12 14">
        <location>
            <begin position="348"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 14">
        <location>
            <begin position="398"/>
            <end position="400"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12 15">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12 15">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12 15">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="438"/>
            <end position="440"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="461"/>
            <end position="462"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="485"/>
            <end position="489"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="570"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="587" mass="63463" checksum="AFC90284640CCF9A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B397</accession>
    <name>A0A673B397_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pterin-4-alpha-carbinolamine dehydratase</fullName>
            <ecNumber evidence="5">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Dimerization cofactor of hepatocyte nuclear factor 1-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Phenylalanine hydroxylase-stimulating protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Pterin carbinolamine dehydratase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity. Also acts as a coactivator for HNF1B-dependent transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673B397"/>
    <dbReference type="FunCoup" id="A0A673B397">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037662.1">
        <property type="protein sequence ID" value="ENSSORP00005036696.1"/>
        <property type="gene ID" value="ENSSORG00005017279.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B397"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00914">
        <property type="entry name" value="PCD_DCoH_subfamily_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.20:FF:000001">
        <property type="entry name" value="Pterin-4-alpha-carbinolamine dehydratase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002018">
        <property type="entry name" value="PRK00823.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002020">
        <property type="entry name" value="PRK00823.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF13">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="127" mass="14652" checksum="391F28610854EA18" modified="2020-06-17" version="1">MTNYNGIRFSQSQRAFVNLPLCDPLQAAKIQSLTEEERNHLLPLLQNAQWVEVVGRDAIYKEFIFKDFNQAFGFMSRVALQAEKMDHHPEWFNVYNKVQITLSTHDCGGLSQRDITLATFIDQASLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3B8</accession>
    <name>A0A673B3B8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B3B8"/>
    <dbReference type="Ensembl" id="ENSSORT00005037687.1">
        <property type="protein sequence ID" value="ENSSORP00005036721.1"/>
        <property type="gene ID" value="ENSSORG00005017295.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3B8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057448">
        <property type="entry name" value="BCL-11A_Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051497">
        <property type="entry name" value="Dev/Hematopoietic_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45993">
        <property type="entry name" value="B-CELL LYMPHOMA/LEUKEMIA 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45993:SF8">
        <property type="entry name" value="ZINC FINGER PROTEIN 296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25491">
        <property type="entry name" value="CCHC_BCL-11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="176" mass="19541" checksum="9335B4AD39A192A3" modified="2020-06-17" version="1">EIRIGSSDQQPAPPALVASDEGSDLLTCGQCSQAFPLAHILAFIQHKQGGCRSRNQASNTRAAPPSPANRAQQRIANTELEPGFIELRRGAARDREPSYFTCQQCEGVFPSAWAVLQHAQHTHSFSIYQEDEDEDTDMRSGRGEIKDCWLKLSAYSSLDHRQKQGHDIQQGDYQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B454</accession>
    <name>A0A673B454_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nurim</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nuclear envelope membrane protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nuclear rim protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nrm</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nurim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004116.1">
        <property type="nucleotide sequence ID" value="XM_030148256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B454"/>
    <dbReference type="FunCoup" id="A0A673B454">
        <property type="interactions" value="675"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036247.1">
        <property type="protein sequence ID" value="ENSSORP00005035298.1"/>
        <property type="gene ID" value="ENSSORG00005016671.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428975"/>
    <dbReference type="CTD" id="11270"/>
    <dbReference type="InParanoid" id="A0A673B454"/>
    <dbReference type="OrthoDB" id="10050858at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033580">
        <property type="entry name" value="Nurim-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31040">
        <property type="entry name" value="NURIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31040:SF1">
        <property type="entry name" value="NURIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="275" mass="30918" checksum="7B94AFBF56A39BB8" modified="2020-06-17" version="1">MASVTVRGWALCTVSLLNFAFVFITGAEFINFISFRAIYHNITGETTLCQDSIPWSAALQNSSVLRCVVVDLGLLALFTAQHSLLAWSPVKQACQSVFGALNRTAYCFTTALALQILMRCWQPVTDAPCLWSVRQTPWSIWFPLLCFSLHFLCWAIIFSILMMFDYPELMGIKQVYYDCLGLGDPLSHKSPGAQRLLSHLRHPVCLELMVVLWFLPALSLDRLLLGGALSVYLALAHSLDKQDLAYLCHQVNSDMQRFAEPPAKDQQKNSNHRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5C3</accession>
    <name>A0A673B5C3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="14">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pygl</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 14">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:41733"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer; enzymatically active. Interacts with PPP1R3B; recruits the phosphatase PP1 which dephosphorylates and inactivates PYGL/glycogen phosphorylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A673B5C3"/>
    <dbReference type="Ensembl" id="ENSSORT00005038462.1">
        <property type="protein sequence ID" value="ENSSORP00005037486.1"/>
        <property type="gene ID" value="ENSSORG00005017363.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5C3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04300">
        <property type="entry name" value="GT35_Glycogen_Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000005">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000153">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000197">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF3">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE, LIVER FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 13 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="875" mass="100337" checksum="18D88610D1AA215E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5V8</accession>
    <name>A0A673B5V8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iroquois homeobox 6a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">irx6a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B5V8"/>
    <dbReference type="Ensembl" id="ENSSORT00005036990.1">
        <property type="protein sequence ID" value="ENSSORP00005036032.1"/>
        <property type="gene ID" value="ENSSORG00005017010.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5V8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF47">
        <property type="entry name" value="IROQUOIS HOMEOBOX PROTEIN 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="139"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="141"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="481" mass="52259" checksum="EE55DFE5BDEE0DFF" modified="2020-06-17" version="1">MQFFVSANPSTTCCDSISRSVSDGTGGSQTAAAAAAAAASFCCPSYENRLLASSRTELNAALGMYSSPYAAAAAASQNYANYFPYSTDPSAIYSSLNPQYDIKDSTGTLHSGITQTAAYYPYDHSLGQYQYDRYGTVDFNGTARRKNATRETTSTLKTWLYEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWSPKNKAGDDRKDDLNKNSSDCKEEKELHLSDLEDIDDDECDKLDSDCEKVAGDEQDLQRAMSVSQAPQKRDCGSELHLSLTNSFHTFPCAIKNVTTLPPLPSDFLDPVVSKATSSSGPTGTVSLSHFESSDKPRIWSLARTAASGVILSPQQQGSELRTGNSACQLESTRLPGAPTGQCAGMRGLHESSGVTNAENSFPESSSLHSKVYGTGSYSHKGIQLHCSSYAALPDTYQYSTIEGFSGDKAETQSSDLSEACGTLQDDKVTAFRPVMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6C1</accession>
    <name>A0A673B6C1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115437504</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B6C1"/>
    <dbReference type="FunCoup" id="A0A673B6C1">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038359.1">
        <property type="protein sequence ID" value="ENSSORP00005037385.1"/>
        <property type="gene ID" value="ENSSORG00005017191.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B6C1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000058">
        <property type="entry name" value="Disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000049">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019590">
        <property type="entry name" value="DLG1_PEST_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF5">
        <property type="entry name" value="DISKS LARGE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10608">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01277">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="192"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="283"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="433"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="548"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="697"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="384"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="398"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="859" mass="95303" checksum="0BC76847CB77E2BA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6I6</accession>
    <name>A0A673B6I6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.205</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2'-O-ribose RNA methyltransferase TRM7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ftsj1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42396"/>
            <dbReference type="Rhea" id="RHEA-COMP:10246"/>
            <dbReference type="Rhea" id="RHEA-COMP:10247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.205"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with WDR6; the interaction is direct, and required for 2'-O-methylation of position 34 in substrate tRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.205" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A673B6I6"/>
    <dbReference type="Ensembl" id="ENSSORT00005038419.1">
        <property type="protein sequence ID" value="ENSSORP00005037445.1"/>
        <property type="gene ID" value="ENSSORG00005017571.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B6I6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106340">
        <property type="term" value="F:tRNA (guanosine(34)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000040">
        <property type="entry name" value="Putative ribosomal RNA methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03162">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028590">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="11">
        <location>
            <begin position="21"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="324" mass="35964" checksum="8319DB0905B093CF" modified="2020-06-17" version="1">MGRSSKDKRDIYYRLAKEEGWRARSAFKLLQLDHEFNLFTGVNRAVDLCAAPGSWSQVLSREEKVEKGEGGEEVKIVAVDLQAMAPLPGVTQIQGDITKVSTAQEIIRHFEGQPADLVVCDGAPDVTGLHDVDEYIQAQLLLAALNITTHVLKPGGTFVAKIFRGKDVTLLYSQLKIFFSGVTCAKPRSSRNSSIEAFVVCQNYQPPEGYVPNMSNPLLDHSYDVDFNQLEGPNRVIVPFLACGDLSAFDSDRTYPLQLDAGKQYQYTPPTQPPIHPPYQQACHLRKNNLLSREDAPTDCPNQSRDQDPSADNICLNDSHCLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9F6</accession>
    <name>A0A673B9F6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Exostosin-like 2</fullName>
            <ecNumber evidence="16">2.4.1.223</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Alpha-1,4-N-acetylhexosaminyltransferase EXTL2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Alpha-GalNAcT EXTL2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">EXT-related protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">extl2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(alpha-D-GlcNAc-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16221"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="Rhea" id="RHEA-COMP:12574"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="ChEBI" id="CHEBI:132104"/>
            <dbReference type="EC" id="2.4.1.223"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.223" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A673B9F6"/>
    <dbReference type="FunCoup" id="A0A673B9F6">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038080.1">
        <property type="protein sequence ID" value="ENSSORP00005037108.1"/>
        <property type="gene ID" value="ENSSORG00005017430.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B9F6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035248">
        <property type="term" value="F:alpha-1,4-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001888">
        <property type="term" value="F:glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019276">
        <property type="term" value="P:UDP-N-acetylgalactosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000058">
        <property type="entry name" value="Exostosin-like glycosyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052427">
        <property type="entry name" value="Glycosyltrans_GT2/GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47844:SF1">
        <property type="entry name" value="EXOSTOSIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47844">
        <property type="entry name" value="SYNTHASE CPS1, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G02500)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="23">
        <location>
            <begin position="113"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="387" mass="43905" checksum="BB7FA7B783DB7C0D" modified="2020-06-17" version="1">MVDLGRECSFQQGAEFGITTGSPPPTWRLCEVNSLCPQAMRVLCCWMGLRRTHLLWPVLLLLLVGAVLTALLPSAEEHDGGAGLLGVLRRDTVQKENPAQVHHVGSTEEEHRFTIIIQTYNRTDILLKLLNHYQAVPHLQQIIIVWNNIGEPTPLKTWNSLGPHPVPLLFKEQSTNRMRNRLQPFREIDTDAVLMLDDDTLVSVPDISFAFSVWKQFPDQIVGFVPRKHVTTLSGVYSYGSFELQDPETAGGDKYSMVLIGAAFFHRRYLQLFQDQPQAVHALVDETQNCDDIAMNFAVSLYLRKNSRSRSFNRPSGVFVKPVDLRNLEKDASSGYQGMWHRPEHLLQRSYCLNKLVQIYGAMPLCFSNIMVSQFGFPNYANHKSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAI1</accession>
    <name>A0A673BAI1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fibronectin type III domain containing 3Ba</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fndc3ba</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FNDC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BAI1"/>
    <dbReference type="FunCoup" id="A0A673BAI1">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038608.1">
        <property type="protein sequence ID" value="ENSSORP00005037627.1"/>
        <property type="gene ID" value="ENSSORG00005017660.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BAI1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000180">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000185">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000210">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000309">
        <property type="entry name" value="Fibronectin type III domain containing 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000396">
        <property type="entry name" value="Fibronectin type III domain containing 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF11">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING 3BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1182"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="278"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="382"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="478"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="574"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="672"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="765"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="871"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="959"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="1059"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="183"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1207" mass="131908" checksum="D9B993B7D74A43F0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BGG1</accession>
    <name>A0A673BGG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BGG1"/>
    <dbReference type="Ensembl" id="ENSSORT00005042716.1">
        <property type="protein sequence ID" value="ENSSORP00005041646.1"/>
        <property type="gene ID" value="ENSSORG00005019358.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BGG1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015631">
        <property type="entry name" value="CD2/SLAM_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080:SF134">
        <property type="entry name" value="CD48 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025488765" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="28"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27324" checksum="898E208312FF0B09" modified="2020-06-17" version="1">GADLTVFKAGLWTAVFLFVLQLTHISAKNIPTYFRPGGTLSLSPTAVPDPVRSVLWTHNGNLVAEWIKDEIELQYYGVFRNRTELNTTTGQLRITQTTEADNGEFSVELNNVIQADRYEARMIQEVPDPYVWVRPNKDLYVLYCDPVPGQEAALQAAEPVSYWWKIGTADWAEFGNKLEVNDNETTRRAETFTCKMVNPVSEKTSQPQKKKSCCRQINVTINCQTLFILLQQQKHLNSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BHK7</accession>
    <name>A0A673BHK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">lysoplasmalogenase</fullName>
            <ecNumber evidence="6">3.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tmem86a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = a 2,3-saturated aldehyde + sn-glycerol 3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:22544"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = a 2,3-saturated aldehyde + sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:16905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
            <dbReference type="ChEBI" id="CHEBI:143890"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM86 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_029992055.1">
        <property type="nucleotide sequence ID" value="XM_030136195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BHK7"/>
    <dbReference type="FunCoup" id="A0A673BHK7">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043118.1">
        <property type="protein sequence ID" value="ENSSORP00005042046.1"/>
        <property type="gene ID" value="ENSSORG00005019499.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420723"/>
    <dbReference type="CTD" id="144110"/>
    <dbReference type="InParanoid" id="A0A673BHK7"/>
    <dbReference type="OrthoDB" id="2133758at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047408">
        <property type="term" value="F:alkenylglycerophosphocholine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012506">
        <property type="entry name" value="TMEM86B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885">
        <property type="entry name" value="GH04784P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885:SF10">
        <property type="entry name" value="LYSOPLASMALOGENASE-LIKE PROTEIN TMEM86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07947">
        <property type="entry name" value="YhhN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="237" mass="26186" checksum="2B03C1A1D1280760" modified="2020-06-17" version="1">MVSPVTVVKSEGPKLVPFFKATCVYFVLWLPTSSPSWFSALIKCLPIFCLWVFLLAHGFSFLGAHSSARKILAGLIFSALGDAFLIWQEQGYFVHGLLMFAITHILYSSAFGMKPLNLRAGLVITAVSTLSYILLYPYLSGPFTYLVAVYIALIGFMGWRAIAGLQLTNDLWTWTKLSACLGAVLFMVSDLTIAVNKFCFPVPHSRAIIMATYYAAQMLIALSAVECQDVEVARKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIL1</accession>
    <name>A0A673BIL1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">smurf2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A673BIL1"/>
    <dbReference type="FunCoup" id="A0A673BIL1">
        <property type="interactions" value="919"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042652.1">
        <property type="protein sequence ID" value="ENSSORP00005041585.1"/>
        <property type="gene ID" value="ENSSORG00005018143.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BIL1"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd08382">
        <property type="entry name" value="C2_Smurf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000047">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000024">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000014">
        <property type="entry name" value="E3 ubiquitin-protein ligase SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000007">
        <property type="entry name" value="E3 ubiquitin-protein ligase SMURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF300">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SMURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 14 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="18">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="255"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="20">
        <location>
            <begin position="436"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="212"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="15 16">
        <location>
            <position position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="770" mass="88184" checksum="CCC7F2329309C168" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJH6</accession>
    <name>A0A673BJH6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="18">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">man1a2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A673BJH6"/>
    <dbReference type="FunCoup" id="A0A673BJH6">
        <property type="interactions" value="1644"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041810.1">
        <property type="protein sequence ID" value="ENSSORP00005040757.1"/>
        <property type="gene ID" value="ENSSORG00005018767.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJH6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000002">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF40">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 18" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="154"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="521"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="546"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="632"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="475"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="655" mass="74029" checksum="0FD0F712774DFE39" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQ52</accession>
    <name>A0A673BQ52_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BTB/POZ domain-containing protein KCTD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">kctd3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Accessory subunit of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) up-regulating its cell-surface expression and current density without affecting its voltage dependence and kinetics.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with HCN3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KCTD3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BQ52"/>
    <dbReference type="FunCoup" id="A0A673BQ52">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046005.1">
        <property type="protein sequence ID" value="ENSSORP00005044871.1"/>
        <property type="gene ID" value="ENSSORG00005020631.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQ52"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000205">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000038">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047876">
        <property type="entry name" value="SHKBP1/KCTD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15859:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15859">
        <property type="entry name" value="SETA BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="13">
        <location>
            <begin position="19"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="140"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="695"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="740"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="838"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="872" mass="94390" checksum="3FB6875FC4ED4C10" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTF5</accession>
    <name>A0A673BTF5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Family with sequence similarity 107 member B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fam107b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029992410.1">
        <property type="nucleotide sequence ID" value="XM_030136550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BTF5"/>
    <dbReference type="FunCoup" id="A0A673BTF5">
        <property type="interactions" value="331"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045566.1">
        <property type="protein sequence ID" value="ENSSORP00005044444.1"/>
        <property type="gene ID" value="ENSSORG00005020466.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420923"/>
    <dbReference type="CTD" id="83641"/>
    <dbReference type="InParanoid" id="A0A673BTF5"/>
    <dbReference type="OrthoDB" id="5963205at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009533">
        <property type="entry name" value="FAM107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768">
        <property type="entry name" value="DOWN REGULATED IN RENAL CARCINOMA 1/TU3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768:SF1">
        <property type="entry name" value="PROTEIN FAM107B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06625">
        <property type="entry name" value="DUF1151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="19329" checksum="A1F7577EC5A4DEFC" modified="2020-06-17" version="1">MTTMFRYPVFPHLQDPCDPGLSLSPGRSVMAEPDYLEGDCDELIKPKKLINPVKTSRNHQDLHRELLMNQKRGLAPQNKPELQKVLEKRKREQVLKAQKEEQEAHKKRSDLEIELMKRQQKLEQLELEQQKIEEEQENTPEFVKMKSNLRRTRQETDGEERTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUM2</accession>
    <name>A0A673BUM2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytosolic arginine sensor for mTORC1 subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">castor2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GATS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BUM2"/>
    <dbReference type="FunCoup" id="A0A673BUM2">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044989.1">
        <property type="protein sequence ID" value="ENSSORP00005043873.1"/>
        <property type="gene ID" value="ENSSORG00005020245.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUM2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2130.10:FF:000003">
        <property type="entry name" value="Cytosolic arginine sensor for mTORC1 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2130.10:FF:000004">
        <property type="entry name" value="Cytosolic arginine sensor for mTORC1 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049479">
        <property type="entry name" value="CASTOR1_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040778">
        <property type="entry name" value="CASTOR1_N"/>
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    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026249">
        <property type="entry name" value="CASTOR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051719">
        <property type="entry name" value="CASTOR_mTORC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31131">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31131:SF2">
        <property type="entry name" value="CYTOSOLIC ARGININE SENSOR FOR MTORC1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21389">
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    </dbReference>
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        <property type="entry name" value="GATSLIKEFMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CASTOR ACT" evidence="4">
        <location>
            <begin position="73"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosolic arginine sensor for mTORC1 subunit 1/2 ACT-like" evidence="6">
        <location>
            <begin position="177"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="CASTOR ACT" evidence="4">
        <location>
            <begin position="261"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="342" mass="37583" checksum="D9E59C10AB6E3A99" modified="2020-06-17" version="1">MELHILEHSLKVASIEKEGIQICTHGLIKLAFLASKTRCKFFSLTETPEDYTIIVDEEGFKELPESEHISVADATWLALNVVSGGGNASNSQPIGVTKIAKSVIAPLADHNISVFMLSTYQTDFILVRERDLPMVMHTLSSEFTLLRVINGETVAAHNLGVTNGFVKPKLVPRPIIHPLSSPSNMFCVTSLDPDTLPSVATLLMDVMFYSGGLKDTGASGEDSSHIRFFSFSLIEGYISLVMDEQTTQRFPNNVLFTSASGELWKMVRIGGQPLGFDECGIVAQISEPLATADIPAYYISTFKFDHALVRDDALYHVFSKVSKLGKHFKHYYTVSGNDTPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BV54</accession>
    <name>A0A673BV54_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Coiled-coil domain containing 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BV54"/>
    <dbReference type="Ensembl" id="ENSSORT00005045177.1">
        <property type="protein sequence ID" value="ENSSORP00005044058.1"/>
        <property type="gene ID" value="ENSSORG00005020309.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BV54"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029336">
        <property type="entry name" value="DUF4594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15635">
        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15635:SF11">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15266">
        <property type="entry name" value="DUF4594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="215"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="40391" checksum="A053A67C20E3F21A" modified="2020-06-17" version="1">MSSAVDLKSKEEKDAELDKRIEALRKKNEALVKRYQEIEEDKKKAEQEGIAVTTPRKPRPHESETDRRKTEKENFTVTVDLSKPTGEKRVVNDWKPSTPRGRKTSEESDGQRGPSPPGGRVRPGSAEEDVEDGEGRGGGGEEEGRRRGGDGGGGRQERRDREPRTPRDGEPGEMGGPGRRGGRRGRGGGGGGGGGGGGGGGGGGGGGGGGTPGGMDRKSKEWEEKRRQNIEKMNEEMERIAEYERGQRPDGDKPIRNFLDDPRRSGPAPDTDRKEGSRRHVRNWGGLDFDNVKTGAELEKEWTSRRPGPKGSVDMTMSMTGRERAEYLRWKKEREQIDEERLARHRNATGQWRREWDAQKTDNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BY63</accession>
    <name>A0A673BY63_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">chp1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the calcineurin regulatory subunit family. CHP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029981766.1">
        <property type="nucleotide sequence ID" value="XM_030125906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BY63"/>
    <dbReference type="FunCoup" id="A0A673BY63">
        <property type="interactions" value="912"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047134.1">
        <property type="protein sequence ID" value="ENSSORP00005045979.1"/>
        <property type="gene ID" value="ENSSORG00005021081.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413162"/>
    <dbReference type="CTD" id="11261"/>
    <dbReference type="InParanoid" id="A0A673BY63"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="EG:114D9.1 PROTEIN-RELATED"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
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            <end position="61"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="195" mass="22507" checksum="839560657C3FBC6B" modified="2020-06-17" version="1">MGSRASTLLREEEIEEIKKETGFSHSQITRLYSRFTSLDKGENGTLSREDFQRIPELAINPLGDRIINAFFPEGEDQVNFRGFMRTLAHFRPIEDNEKNKNSSASEPLNSRTNKLLFAFRLYDLDRDDKISRDELLQVLRMMVGVNISDEQLGSIADRTIQEADTNGDNSISFNEFIKVLEKVDVEQKMSIRFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1S4</accession>
    <name>A0A673C1S4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="28">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">LOC115430255</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:46681"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 27 28">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MTARC2. Interacts with MTLN; the interaction is required to maintain cellular lipid composition and leads to stimulation of mitochondrial respiratory complex I activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Endoplasmic reticulum membrane</location>
            <topology evidence="23">Lipid-anchor</topology>
            <orientation evidence="23">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Mitochondrion outer membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
            <orientation evidence="24">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 28">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="28"/>
    <dbReference type="RefSeq" id="XP_030006059.1">
        <property type="nucleotide sequence ID" value="XM_030150199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C1S4"/>
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    <dbReference type="Ensembl" id="ENSSORT00005047467.1">
        <property type="protein sequence ID" value="ENSSORP00005046308.1"/>
        <property type="gene ID" value="ENSSORG00005020968.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430255"/>
    <dbReference type="CTD" id="1727"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial outer membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:FAD binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cholesterol biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
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        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
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        <property type="entry name" value="CBR-like"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
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    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF121">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 27 28" id="KW-0274">FAD</keyword>
    <keyword evidence="6 27 28" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15 28" id="KW-0520">NAD</keyword>
    <keyword evidence="13 28" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="14" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="29">
        <location>
            <begin position="38"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
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        <location>
            <position position="91"/>
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            <name>FAD</name>
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        <location>
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        <ligand>
            <name>FAD</name>
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        </ligand>
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        <location>
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        </ligand>
    </feature>
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        <location>
            <position position="109"/>
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        <ligand>
            <name>FAD</name>
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        </ligand>
    </feature>
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        <location>
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        </ligand>
    </feature>
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        <location>
            <position position="124"/>
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        <ligand>
            <name>FAD</name>
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        </ligand>
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        </ligand>
    </feature>
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        </ligand>
    </feature>
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        <location>
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            <name>FAD</name>
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        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022516"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022787"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00037811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00037821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="299" mass="33942" checksum="3D3D57C71CFE9283" modified="2020-06-17" version="1">MLSYILGLIRGGIDSIINLIFRLFFSKRRPAITLEDPNIKYALRLIDKQIVSHDTRRFRFALPSPDHVLGLPIGQHIYLSARIDGKLVVRPYTPVSSDDDKGFVDLVVKVYFKDVNPKFPEGGKMSQYLESLKINDTIDFRGPSGLLIYKGKGVFHIQPEKKSPAVTKTAKYVGMIAGGTGITPMLQLITAVMKDPEDQTVCYLLFANQTEKDILLRPELEEIQVNHPDRFKLWFTLDRAPDNWEYSQGFINEDMVREQLPPPSDDTLILMCGPPPMIQFACNPNLDKVGHSSSRRFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C254</accession>
    <name>A0A673C254_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amine oxidase</fullName>
            <ecNumber evidence="10">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">si:ch211-127i16.2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a secondary aliphatic amine + O2 + H2O = a primary amine + an aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:26414"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:58855"/>
            <dbReference type="ChEBI" id="CHEBI:65296"/>
            <dbReference type="EC" id="1.4.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A673C254"/>
    <dbReference type="Ensembl" id="ENSSORT00005050547.1">
        <property type="protein sequence ID" value="ENSSORP00005049346.1"/>
        <property type="gene ID" value="ENSSORG00005022414.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C254"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097621">
        <property type="term" value="F:monoamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050703">
        <property type="entry name" value="Flavin_MAO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563:SF14">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="11">
        <location>
            <begin position="15"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="535" mass="58701" checksum="1B5E2C7E7328CF8C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3D3</accession>
    <name>A0A673C3D3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Muscleblind like splicing regulator 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the muscleblind family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C3D3"/>
    <dbReference type="Ensembl" id="ENSSORT00005048615.1">
        <property type="protein sequence ID" value="ENSSORP00005047437.1"/>
        <property type="gene ID" value="ENSSORG00005021687.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C3D3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000004">
        <property type="entry name" value="Muscleblind like splicing regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000002">
        <property type="entry name" value="Muscleblind-like 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054429">
        <property type="entry name" value="Znf-CCCH_Muscleblind-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF4">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22628">
        <property type="entry name" value="zf-CCCH_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="393" mass="42918" checksum="6207E551C7CBED0A" modified="2020-06-17" version="1">MALNISSMRDTKWLTLEVCRQFQRGNCSRSDEECKFAHPPKSCQIENGRVIACFDSLKGRCSRENCKYLHPPSHLKTQLEINGRNNLIQQKTAAAMLTQQMQLMIPGPSLQPLVRPSTTPQGLGTNTGLGYGTYMTPLSHAMNLVPSDILPSTSVLVPGSPSVTVPSSSSSSSSSSSPSQKLHRTDKLEVCREFQRGNCARGETDCRFAHPSDSPMIDANDNTVTVCMDYVKSRCSREKCKYFHPPAHLQAKIKSSQQQVSQTAVAAQTAAAAVTQSTAKAMKRPLEATLDLAFPHSVLQPLPKRQALEKSSWTSSLLNPSFLHYQQALANTQLQQPTAAFYPTVPMMYSATPATVSAATTPATGVPYAATAPANQVWILKTKVIQSYRLIFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3T5</accession>
    <name>A0A673C3T5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115435334</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="RefSeq" id="XP_030013534.1">
        <property type="nucleotide sequence ID" value="XM_030157674.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030013544.1">
        <property type="nucleotide sequence ID" value="XM_030157684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C3T5"/>
    <dbReference type="FunCoup" id="A0A673C3T5">
        <property type="interactions" value="1068"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049089.1">
        <property type="protein sequence ID" value="ENSSORP00005047904.1"/>
        <property type="gene ID" value="ENSSORG00005021120.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435334"/>
    <dbReference type="InParanoid" id="A0A673C3T5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10162">
        <property type="entry name" value="ClassIIa_HDAC4_Gln-rich-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000002">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046949">
        <property type="entry name" value="HDAC4/5/7/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF13">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037911">
        <property type="entry name" value="HDAC_II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="18">
        <location>
            <begin position="86"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="17">
        <location>
            <begin position="689"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="230"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="535"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="638"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1062"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="639"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1063"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="811"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="681"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="683"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="689"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="759"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Contributes to catalysis" evidence="15">
        <location>
            <position position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1093" mass="119768" checksum="E26C86F8B98E1C6C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C999</accession>
    <name>A0A673C999_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biogenesis of lysosomal organelles complex-1, subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bloc1s2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C999"/>
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        <property type="interactions" value="134"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051037.1">
        <property type="protein sequence ID" value="ENSSORP00005049834.1"/>
        <property type="gene ID" value="ENSSORG00005022613.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C999"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="193" mass="21393" checksum="31721FF18ECC24E1" modified="2020-06-17" version="1">MWAHVTWLKQNRNKMAATGDDAAAMDSISRAPTTHSAALNPAGTSSGQGECPVDATEAAACAPKRPHTNSDGGVETAEEAVEPAEPDINELCTDMFEKMAIFLQGELTGTCEDYRLLENMNKLTSLKYMEMKDISINISRNLQDLNNKYASLQPYLDQINQIEEQVSALEQAAYKLDAYSKKLEARFKKLEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9X6</accession>
    <name>A0A673C9X6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Twinfilin actin-binding protein 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115418849</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C9X6"/>
    <dbReference type="FunCoup" id="A0A673C9X6">
        <property type="interactions" value="485"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051295.1">
        <property type="protein sequence ID" value="ENSSORP00005050089.1"/>
        <property type="gene ID" value="ENSSORG00005022729.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C9X6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010591">
        <property type="term" value="P:regulation of lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11284">
        <property type="entry name" value="ADF_Twf-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11285">
        <property type="entry name" value="ADF_Twf-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000007">
        <property type="entry name" value="Twinfilin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000012">
        <property type="entry name" value="Twinfilin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028458">
        <property type="entry name" value="Twinfilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759:SF9">
        <property type="entry name" value="TWINFILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ADF-H" evidence="10">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="ADF-H" evidence="10">
        <location>
            <begin position="184"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="327"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="356" mass="41080" checksum="2922B096F45DCFED" modified="2020-06-17" version="1">MSHQTGINATPELKEFLARARGGAIRIMKIIIRNEELVLDSCREPTQSWDKDYDQFLLPLLTPQEPCYILYRLDSQNAQGYEWIFIAWSPDQSPVRQKMVYAATRATLKKEFGGGHIKDEVFGTVEDDLCFQGYLRHVSSHSAPAPLTSAEQELQRIKVTEVQHKETTVTMEFGLDKRAQTLQGLAFPLQEEAKRALQQLKQKRINYIQLRLDVEKETIELVHTKPTETHELPYRIPTDSPRYHFFIFKHSHQGQLQEALVFIYSMPGYTCSIKERMLYSSCKNRLLDEVERDYQLEVTKKMEIDSGDGLTEEFLYEEVHPMEHTLKQAFSKPRGPGGKRGNKRLIKGAGENGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAC0</accession>
    <name>A0A673CAC0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Cadherin EGF LAG seven-pass G-type receptor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">celsr2</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor that may have an important role in cell/cell signaling during nervous system formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005050310.1">
        <property type="protein sequence ID" value="ENSSORP00005049113.1"/>
        <property type="gene ID" value="ENSSORG00005022323.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CAC0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120036">
        <property type="term" value="P:plasma membrane bounded cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15992">
        <property type="entry name" value="7tmB2_CELSR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000011">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000059">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000112">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000005">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000047">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000156">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000020">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000005">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000089">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000010">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000023">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000029">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000038">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000040">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000113">
        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000044">
        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000003">
        <property type="entry name" value="Putative cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="9"/>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBF8"/>
    <dbReference type="FunCoup" id="A0A673CBF8">
        <property type="interactions" value="1113"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005050082.1"/>
        <property type="gene ID" value="ENSSORG00005022715.1"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:small GTPase-mediated signal transduction"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000095">
        <property type="entry name" value="Rho-related GTP-binding protein RhoC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
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        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
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    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="197" mass="22280" checksum="1E7B8373DF0506CC" modified="2020-06-17" version="1">MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDSKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEDGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKSSKLLKPCLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBU5</accession>
    <name>A0A673CBU5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CBU5"/>
    <dbReference type="Ensembl" id="ENSSORT00005050810.1">
        <property type="protein sequence ID" value="ENSSORP00005049608.1"/>
        <property type="gene ID" value="ENSSORG00005022517.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBU5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="246" mass="27798" checksum="DDB94F89CEB4BC10" modified="2020-06-17" version="1">MYSSYQDREEIEDDLYREEEGDSEGSEVNSEVEFRLYSQLHYSSNAVEDEEEKEHEEKSQDSWPLEVTANDDAEIGQSTESRTLSHKPREQQKPLKAGTKQKTGKGDEFKGKGASKGKRPSSSVFEEVIVIDSDPDVITLSEDDTAEDDDGVCAVKGRQLRTSTPAQKVGILFASDVQCPHFYIRVRGAMVQVAHSTVRTSVFGPLFWFGFGTCFVRKENCFFFSPKITFLFCLPENFCNGAPFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCI3</accession>
    <name>A0A673CCI3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCI3"/>
    <dbReference type="Ensembl" id="ENSSORT00005054546.1">
        <property type="protein sequence ID" value="ENSSORP00005053291.1"/>
        <property type="gene ID" value="ENSSORG00005023959.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054547.1">
        <property type="protein sequence ID" value="ENSSORP00005053292.1"/>
        <property type="gene ID" value="ENSSORG00005023960.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="term" value="C:adherens junction"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:catenin complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cadherin binding"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF106">
        <property type="entry name" value="CADHERIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="84" mass="8849" checksum="F1F03F3E6E7C5249" modified="2020-06-17" version="1">METSLVLVVADGGEPPLSSTATLHIRVCVCQRNTRGRNGNNVCQAQAFLSSAGLSTGAFVAILLCIVILLGKYVSARGEYCSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCN5</accession>
    <name>A0A673CCN5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase TRIM21-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115438559</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030018107.1">
        <property type="nucleotide sequence ID" value="XM_030162247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CCN5"/>
    <dbReference type="Ensembl" id="ENSSORT00005053083.1">
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CIB5</accession>
    <name>A0A673CIB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">galnt18b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Golgi apparatus membrane</location>
            <topology evidence="2 18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A673CIB5"/>
    <dbReference type="FunCoup" id="A0A673CIB5">
        <property type="interactions" value="980"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055200.1">
        <property type="protein sequence ID" value="ENSSORP00005053930.1"/>
        <property type="gene ID" value="ENSSORG00005024217.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CIB5"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000017">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000012">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF37">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 18" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="19">
        <location>
            <begin position="159"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="580" mass="66549" checksum="2DFB094600AE5E80" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJ93</accession>
    <name>A0A673CJ93_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HPS4 biogenesis of lysosomal organelles complex 3 subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hps4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029999934.1">
        <property type="nucleotide sequence ID" value="XM_030144074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CJ93"/>
    <dbReference type="Ensembl" id="ENSSORT00005053984.1">
        <property type="protein sequence ID" value="ENSSORP00005052732.1"/>
        <property type="gene ID" value="ENSSORG00005023763.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426108"/>
    <dbReference type="CTD" id="89781"/>
    <dbReference type="InParanoid" id="A0A673CJ93"/>
    <dbReference type="OrthoDB" id="16754at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031085">
        <property type="term" value="C:BLOC-3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903232">
        <property type="term" value="P:melanosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026091">
        <property type="entry name" value="HPS4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407:SF9">
        <property type="entry name" value="BLOC-3 COMPLEX MEMBER HPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME 4 PROTEIN LIGHT-EAR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="2">
        <location>
            <begin position="13"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="3">
        <location>
            <begin position="492"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="601" mass="65745" checksum="A565C183CAEAE56C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKD0</accession>
    <name>A0A673CKD0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kish</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tmem167a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Golgi apparatus membrane</location>
            <topology evidence="2 9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the KISH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CKD0"/>
    <dbReference type="FunCoup" id="A0A673CKD0">
        <property type="interactions" value="1079"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055016.1">
        <property type="protein sequence ID" value="ENSSORP00005053749.1"/>
        <property type="gene ID" value="ENSSORG00005024150.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKD0"/>
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    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030125307.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSSORT00005059025.1">
        <property type="protein sequence ID" value="ENSSORP00005057706.1"/>
        <property type="gene ID" value="ENSSORG00005025552.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="9945342at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025533171" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="42"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="243" mass="27298" checksum="27B2AC823028B4D0" modified="2020-06-17" version="1">MESPKKEDCSRLLLLCVTVLCLHVQLAVAGECPADGRMWVPFGDRCYHFVHGEEDRIKSYTFESAKTLCKGFELLTVQSAEENDFVIKYSPEVWKNNVNVWLGMYYDTTSENMKWFGEELINYTNWEASTSPSDLVPIDSCVALHSSTGKWEMVSCLDQVENGVVCEAAQKAEEAKKKPNALLSALVILSVVVIMGVSAVVWFLHQKHNLGSTVFTAFEYHPPFRVPDADQSCLVEAEETDMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQB9</accession>
    <name>A0A673CQB9_9TELE</name>
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        <submittedName>
            <fullName evidence="10">Adhesion G-protein coupled receptor G5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115415178</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029984531.1">
        <property type="nucleotide sequence ID" value="XM_030128671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CQB9"/>
    <dbReference type="Ensembl" id="ENSSORT00005057767.1">
        <property type="protein sequence ID" value="ENSSORP00005056470.1"/>
        <property type="gene ID" value="ENSSORG00005025122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415178"/>
    <dbReference type="InParanoid" id="A0A673CQB9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF474">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025392580" evidence="7">
        <location>
            <begin position="24"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="8">
        <location>
            <begin position="256"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
        <location>
            <begin position="413"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="702" mass="77894" checksum="96DE9CB4D0A14308" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CTC8</accession>
    <name>A0A673CTC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mazb</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CTC8"/>
    <dbReference type="Ensembl" id="ENSSORT00005057104.1">
        <property type="protein sequence ID" value="ENSSORP00005055817.1"/>
        <property type="gene ID" value="ENSSORG00005024884.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CTC8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000780">
        <property type="entry name" value="myc-associated zinc finger protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001550">
        <property type="entry name" value="myc-associated zinc finger protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000095">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000108">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000305">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="302"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="505" mass="54849" checksum="5DDE491D351148C6" modified="2020-06-17" version="1">MDAAWSNFLFQNTPTQNQVDGSLQSELMTVHTSSPQTPPTEHIAQPPSTVDTAALSEEPMPVKPVSRPARVPHICAICNKQFKNNYNLRRHQSVHTGVRMKDRAREQAEGAKEGAVGRVERTTVPLSLLHLSAPPPLAPPGVLAGAQHPPLVSQDGEGVAMPNVMASVNPHAPPPAAVVMATGATVQVGQGFVEMAVINPKTFKSGNYFAKCPCTGFSSGPVNSIPCVPQRPANSNPNPVRKNHACETCGKAFRDVYHLNRHRLSHSDEKPFSCPICQQRFKRKDRMSHHVRSHQGGVEKPYVCPHCGKAFSRPDHLNSHVRQVHSSERPFKCPTCESSFATKDRLRAHMIRHEEKVPCHICGKLLSAAYITDHMRVHNQSQHHACHLCNRSFTTLTYLRVHAQKHHGQEWKDSPGGFGGTTSGGVLVCHLCGVHCKTPTQLQGHMGTHSNNQASPNPVTSSVASSSSVSLSNMVTAPTVYVTGNTVVDLLVTDCSSIAAPQPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CVP9</accession>
    <name>A0A673CVP9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Hexosyltransferase</fullName>
            <ecNumber evidence="17">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">b3galt1b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP-alpha-D-galactose = a beta-D-Gal-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1')-Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16045"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17103"/>
            <dbReference type="ChEBI" id="CHEBI:17292"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.86"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16046"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an N-acetyl-beta-D-glucosaminyl derivative + UDP-alpha-D-galactose = a beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-glucosaminyl derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61631"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:133506"/>
            <dbReference type="EC" id="2.4.1.86"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:53433"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Golgi apparatus membrane</location>
            <topology evidence="2 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A673CVP9"/>
    <dbReference type="Ensembl" id="ENSSORT00005059826.1">
        <property type="protein sequence ID" value="ENSSORP00005058500.1"/>
        <property type="gene ID" value="ENSSORG00005025813.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CVP9"/>
    <dbReference type="OrthoDB" id="5957813at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF20">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025402574" description="Hexosyltransferase" evidence="18">
        <location>
            <begin position="27"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="328" mass="38366" checksum="31556E25C4F586EF" modified="2020-06-17" version="1">MPSKVSCLYLLTVVCWASALWYLSISRPTSNYVGHMSVPMRKIAKPPKNITFTNIRTRPLNPHAFEFLINEPKKCESVAPFLVILISTTHKEFDARQAIRETWGDESTFSDIRILTIFLLGKNTDSVLNQMVEQESQIFHDIVVENFIDSYHNLTLKTMMGMRWVATYCPKAQYIMKTDSDIFVNMDNLIYKLLKPTTKPRRRYFTGYVINGGPIRDMRSKWYMSRDLYPDSKYPPFCSGTGYVFSADVAELIYKTSLHTRLLHLEDVYVGLCLRKLGIHPYQNSGFNHWKMAYSLCRYRRVITVHQISPEEMHRIWNDMSSKKHLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CX61</accession>
    <name>A0A673CX61_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">PHD finger protein 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">phf23b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a negative regulator of autophagy.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response. Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1. Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis. Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation. Essential for testicular stem-cell differentiation and sustained spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the PHF23 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CX61"/>
    <dbReference type="FunCoup" id="A0A673CX61">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059552.1">
        <property type="protein sequence ID" value="ENSSORP00005058229.1"/>
        <property type="gene ID" value="ENSSORG00005025725.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CX61"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902902">
        <property type="term" value="P:negative regulation of autophagosome assembly"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD finger protein 13"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD FINGER PROTEIN 23"/>
        <property type="match status" value="1"/>
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    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="264"/>
            <end position="308"/>
        </location>
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        <location>
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        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SMART" id="SM00249"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058229.1"/>
        </source>
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    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="320" mass="35055" checksum="B23A18DD453BA722" modified="2020-06-17" version="1">MLDIMSEHQDSLLTCKTEPLPPERKKRTVEDFNKFCSFVLAYAGYIPSPKEESSWSPTSSSSAHSSGLSADGGGGGGSSSMGNTWGDGSSDIHTIHTFVRKARANKGKNIRRMHSDSSLLDKMRLKDSLYETQASAKAERKKEKKLKKLSLGSAMTAADSGSSEGEKRARIKRSKNSKQPKAKKLKSSAGQSETDSEARHSEVRSVRDELATHGGTTPTAQGLPKTPPDESIRDAEMSSSEGETWIADEDIMVESGDDSWDLITCYCGKPFAGRPMIECNQCSIWVHLSCAKIKKSNVPDIFYCHKCRDLRRSGGHKKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4Q3</accession>
    <name>A0A672Y4Q3_9TELE</name>
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        <recommendedName>
            <fullName evidence="13">Inter-alpha-trypsin inhibitor heavy chain 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115418910</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="entry name" value="vWFA_dom_sf"/>
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        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
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    </dbReference>
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        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H4"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <end position="573"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="860" mass="96515" checksum="04638C0C3BFB9BE4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6J7</accession>
    <name>A0A672Y6J7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Stathmin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">stmn1a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the stathmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y6J7"/>
    <dbReference type="FunCoup" id="A0A672Y6J7">
        <property type="interactions" value="593"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001998.1">
        <property type="protein sequence ID" value="ENSSORP00005001943.1"/>
        <property type="gene ID" value="ENSSORG00005001208.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y6J7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030514">
        <property type="entry name" value="Stathmin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000956">
        <property type="entry name" value="Stathmin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036002">
        <property type="entry name" value="Stathmin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10104">
        <property type="entry name" value="STATHMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10104:SF5">
        <property type="entry name" value="STATHMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00836">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002285">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00345">
        <property type="entry name" value="STATHMIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101494">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00563">
        <property type="entry name" value="STATHMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01041">
        <property type="entry name" value="STATHMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51663">
        <property type="entry name" value="STATHMIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="125"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="156" mass="17810" checksum="8BCD71F7D933772A" modified="2020-06-17" version="1">RSTVSPAFVDIQVKELDKRASGQAFEVLLGAPAPDSKGEFPLSPPKKKDVSLEEIQRKLDAAEERRKNHEAEVLKHLAEKREHEKEVQQKAMEENNNFSKMAEEKLNQKMEANKENRTAIMAAMNEKFKEKDKKFEEVRKNKETKEGASEDGASQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAB3</accession>
    <name>A0A672YAB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia and flagella associated protein 221</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YAB3"/>
    <dbReference type="Ensembl" id="ENSSORT00005001178.1">
        <property type="protein sequence ID" value="ENSSORP00005001144.1"/>
        <property type="gene ID" value="ENSSORG00005000689.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAB3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029676">
        <property type="entry name" value="CFAP221"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46500">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46500:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24771">
        <property type="entry name" value="Ig_CFAP74_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="29953" checksum="2733A8099BF99C44" modified="2020-06-17" version="1">FAKMEVTCSAPQMFTEPHRRGTPLPLNPLVEENRSRANVPNHLLESKVYAKLKSNSLIQAEPAEVHFSGFELDKDYLKMLKLINISSEVMNIHIIPTQTKHFQTSYTKKYRLIPGLAYTVKVQFCPDEWRYFYDCIRVHCKGEENLLIPVHAYPVIDDLHIPPHIDLPAIPLGQSHAIPLRCSCPIDFEFQVFVIQPSEAFSIHPLIGVIPACGEVNIMVTFTPVQYETSQVTIQIVVSQFNTKPFLCTITGSSAPYLGIRYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBE9</accession>
    <name>A0A672YBE9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Si:ch1073-174d20.2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115421214</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YBE9"/>
    <dbReference type="FunCoup" id="A0A672YBE9">
        <property type="interactions" value="1235"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000716.1">
        <property type="protein sequence ID" value="ENSSORP00005000690.1"/>
        <property type="gene ID" value="ENSSORG00005000446.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YBE9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1690.20:FF:000001">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF7">
        <property type="entry name" value="AFG3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="21">
        <location>
            <begin position="246"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="666"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="706" mass="77943" checksum="D8E46140E5FAE750" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDD8</accession>
    <name>A0A672YDD8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Medium-chain acyl-CoA ligase ACSF2, mitochondrial</fullName>
            <ecNumber evidence="5">6.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">acsf2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a medium-chain fatty acid + ATP + CoA = a medium-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48340"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:59558"/>
            <dbReference type="ChEBI" id="CHEBI:90546"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>octanoate + ATP + CoA = octanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33631"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672YDD8"/>
    <dbReference type="FunCoup" id="A0A672YDD8">
        <property type="interactions" value="657"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003245.1">
        <property type="protein sequence ID" value="ENSSORP00005003149.1"/>
        <property type="gene ID" value="ENSSORG00005001763.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDD8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="69"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="10">
        <location>
            <begin position="489"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="592" mass="65947" checksum="0B7655D36BA3AF43" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDU4</accession>
    <name>A0A672YDU4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Activin receptor type-1B</fullName>
            <ecNumber evidence="3">2.7.11.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Activin receptor type IB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Activin receptor-like kinase 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Serine/threonine-protein kinase receptor R2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">acvr1ba</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="23">Forms an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Part of a complex consisting of MAGI2/ARIP1, ACVR2A, ACVR1B and SMAD3. Interacts with SMAD2 and SMAD3. Interacts with SMAD7. Interacts with FKBP1A. Interacts with IGSF1. Interacts with CRIPTO. Interacts with TDP2. Interacts with TSC22D1/TSC-22.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672YDU4"/>
    <dbReference type="Ensembl" id="ENSSORT00005001266.1">
        <property type="protein sequence ID" value="ENSSORP00005001231.1"/>
        <property type="gene ID" value="ENSSORG00005000726.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDU4"/>
    <dbReference type="OrthoDB" id="69842at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14143">
        <property type="entry name" value="STKc_TGFbR1_ACVR1b_ACVR1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23536">
        <property type="entry name" value="TFP_LU_ECD_ALK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000045">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000010">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000023">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF22">
        <property type="entry name" value="ACTIVIN RECEPTOR TYPE-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
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            <position position="226"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023211"/>
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            <dbReference type="ARBA" id="ARBA00048773"/>
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            <dbReference type="RuleBase" id="RU000304"/>
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            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="497" mass="56042" checksum="9E2194EA1D0094FF" modified="2020-06-17" version="1">MALKQTALTLLALLGSVAVGNALRCNCTNCEKTGYECETDGACMASTYYIQGKEQHVRICINKDNLVPPGQPFYCLSAEGLLNTHCCYVDYCNSIDLKVPVPTKEWTGPGSSWGPVELVAVIAGPVFLLCVLLMVGVFLFQYHQRAYSHRQRLEVEDPSCDHLYLAKDKTLQDLIYDMSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGKWRGGDVAVKIFSSREERSWFREAEIYQTIMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYSVTIEGMIKLALSAASGLAHLHMEILGTQGKPGIAHRDLKSKNILVKKNGMCAIADLGLAVRHESITDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNTGGIHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNVPNWWQSYESLRVMGKIMRECWYANGAARLTALRIKKTLSQLSVEEDVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF59</accession>
    <name>A0A672YF59_9TELE</name>
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        <submittedName>
            <fullName evidence="11">TPX2 microtubule nucleation factor</fullName>
        </submittedName>
    </protein>
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        <name evidence="11" type="primary">LOC115424013</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPX2 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005003310.1"/>
        <property type="gene ID" value="ENSSORG00005002014.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YF59"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:regulation of mitotic spindle organization"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="TPX2_C"/>
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        <property type="entry name" value="TPX2_central_dom"/>
    </dbReference>
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        <property type="entry name" value="TPX2_fam"/>
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        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
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    </dbReference>
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        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
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        <property type="entry name" value="TPX2_importin"/>
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    <proteinExistence type="inferred from homology"/>
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            <end position="16"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06886"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="576" mass="65973" checksum="29B5A6E2AD0E7508" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YI50</accession>
    <name>A0A672YI50_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">VWFC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fras1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FRAS1 family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A672YI50">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005004367.1">
        <property type="protein sequence ID" value="ENSSORP00005004241.1"/>
        <property type="gene ID" value="ENSSORG00005000669.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YI50"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Complement Module, domain 1"/>
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        <property type="match status" value="6"/>
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        <property type="match status" value="3"/>
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        <location>
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        <source>
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            <dbReference type="PROSITE" id="PS50184"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004241.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="3992" mass="439562" checksum="42880383D4F22FDD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIK4</accession>
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            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="PROSITE" id="PS50994"/>
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            <dbReference type="Ensembl" id="ENSSORP00005004379.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="271" mass="30594" checksum="C24541432A19BE04" modified="2020-06-17" version="1">VWNVKSWVSECVDCASQKSSGRQPCAPLQPSSNSWPFEWVALDILGPLPVTPSGNRYILVIGDYFSKWTEAFPLPNQEAGTIARVLTEEWVFPYITISSPYHPQSDGLIEHFNRTLLSMLSLFVEDNQLNWDTLSPYVMLAYRSSIRASTSFTLYWVLFGREAMLPVYVMLNVGNQEPFTSVDDYVSKLQDTLSSVVIERVTDVLYGLVPVEGGPESVINFNRLKPFASPLPVHSQQVLGPRQRVQTNGEPRPPRGQRPEVLLGARWIHHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKE7</accession>
    <name>A0A672YKE7_9TELE</name>
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        <recommendedName>
            <fullName evidence="12">Relaxin-3 receptor 1</fullName>
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        <alternativeName>
            <fullName evidence="13">Relaxin family peptide receptor 3</fullName>
        </alternativeName>
    </protein>
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        <name evidence="16" type="primary">rxfp3.3b</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
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            <taxon>Apogonoidei</taxon>
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        </lineage>
    </organism>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for RNL3/relaxin-3. Binding of the ligand inhibit cAMP accumulation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSSORG00005002987.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:C-C chemokine binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:C-C chemokine receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell chemotaxis"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000216">
        <property type="entry name" value="Relaxin family peptide receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000248">
        <property type="entry name" value="ATII_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF935">
        <property type="entry name" value="RELAXIN FAMILY PEPTIDE RECEPTOR 3.3A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00241">
        <property type="entry name" value="ANGIOTENSINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="56"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="373" mass="42644" checksum="EDF091239A8DCE06" modified="2020-06-17" version="1">MGELWNHNTTTPWNRSAGQDRFSSLDDIDMPADGSPTLRILISIVYSVVCAAGLVGNLLVFFLMKVRRGRKKRSSINLFILNLAVTDFQFVLTLPFWAVDTALDFSWPFGNAMCKIILSVTVMNMYASVFFLTAMSVTRYWSVASALKDRTRRRVCPVRWVIAGLWVSATVASLPTAIFSTVKSVAGERLCLLGFPDGQSWLALYHLQKILVAFVFPMLIVTVCYLLLLRFVRLRSMNNNQVKRRSRVTRSVTIVVLSFFICWMPNHAITFWGVLVKFNVVNWDKTYYMVHTYIHPVTVCLAHTNSCLNPVLYCLMRREFRKKMKELFWRISSPTGANTCHLRPFSGTVRAERENQPAAASNMTLWSILDIYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YM03</accession>
    <name>A0A672YM03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA damage-inducible transcript 4 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DDIT4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YM03"/>
    <dbReference type="Ensembl" id="ENSSORT00005005888.1">
        <property type="protein sequence ID" value="ENSSORP00005005646.1"/>
        <property type="gene ID" value="ENSSORG00005003424.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YM03"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071889">
        <property type="term" value="F:14-3-3 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.40">
        <property type="entry name" value="RTP801-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012918">
        <property type="entry name" value="RTP801-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038281">
        <property type="entry name" value="RTP801-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478:SF7">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478">
        <property type="entry name" value="DNA-DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN DDIT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07809">
        <property type="entry name" value="RTP801_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="163" mass="18081" checksum="CD5E6368A8F1DCFD" modified="2020-06-17" version="1">IHHFPSPPFPPPVCLSSLLACYQAKYSADGVPLFDWLPRGAEAWLPWGLYKHCCETNRRAVQLLTRGGSKGGNFWKHIGQESFILAASAPCGLRGLMIDLCVEQGDVCENMAELSVDPYLVPTFQLTLVLRLESSGQAIKLSTGFPCYKKKLYCSEDLLIEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMF0</accession>
    <name>A0A672YMF0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aldehyde dehydrogenase 1 family, member A3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aldh1a3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YMF0"/>
    <dbReference type="FunCoup" id="A0A672YMF0">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005926.1">
        <property type="protein sequence ID" value="ENSSORP00005005682.1"/>
        <property type="gene ID" value="ENSSORG00005003418.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YMF0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000050">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="7">
        <location>
            <begin position="38"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="510" mass="55671" checksum="595E8FD7E416C497" modified="2020-06-17" version="1">MAQNGTAQNGPVEGTASVPQPLKVQDIRHTKIFINNEWHTSSTGRTFPTFNPATGVKLCDVEEGDKVRQTGLFMTLSAKAAQQRGSAWRRMDASSRGRLLHRLADLMERDRLLLAVDPGLREPFLQSFYVDLDGSIKTLRYYAGWSDKVHGKSLPIDESFMCLTKHEPVGVCGAIIPWNFPLLMFSWKIGPALSCGNTVVVKPAEETPLTALHMGSLIAEAGFPPGVVNIIPGFGPTAGAAIANHMDIDKVTFTGSTQVGQLIKEAAAKSNLKRVTLELGGKNPCIVFSDCDLQLAVEETHKGAFYNQGQCCTAASRVFVEESIYEEFVLRSVERAKKVVIGDPLDPLTSHGPQISRRQFLKIMDLIDSGKEEGARLEYGGVAVGGAESLFIQPTIFSNLRDHMRIAKEEIFGPVQCIFSFKSQQEVVERANRSPFGLVSAVFTGSVDRALSMSAALDTGTVWVNCYNALHAQTPFGGFKMSGHGRELGEYALAEYSEVKAVTIKLRETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMW0</accession>
    <name>A0A672YMW0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PI-PLC X domain-containing protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">zgc:112023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YMW0"/>
    <dbReference type="Ensembl" id="ENSSORT00005006175.1">
        <property type="protein sequence ID" value="ENSSORP00005005925.1"/>
        <property type="gene ID" value="ENSSORG00005003543.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YMW0"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08616">
        <property type="entry name" value="PI-PLCXD1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042158">
        <property type="entry name" value="PLCXD1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF112">
        <property type="entry name" value="PI-PLC X DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="313" mass="35465" checksum="0E63A5A431FAC7CD" modified="2020-06-17" version="1">MEDKGRVQVEGDPAEWMSRLPEELLDVPLWNLAIPGSHDSMSFCLDVSSPVLKSEPCLLRLMDRLLPCFTRPCIYRWATTQQSVLSDQCDLGIRFLDLRLARKPAGGSKLFFAHGIYTVMTVKEALDELAVWLDAHPREVVIVFCSHFESLTDSDHTHLVEYIIGLFGQKLCSSQDRPTLRSCWVKGQQVILSYDDQQMVLQHPELWPGIPYWYADSPDPTKVIKYLEDQKSKGRPTGFYVSGLNLTEDAAYVVLHPLQDMRKMTLKALSLLLGWAAEQRPGPQASAINVLCCDFVDVSRFCSLVISLNYKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTD7</accession>
    <name>A0A672YTD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Crystallin, beta B1, like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">crybb1l2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YTD7"/>
    <dbReference type="Ensembl" id="ENSSORT00005008050.1">
        <property type="protein sequence ID" value="ENSSORP00005007767.1"/>
        <property type="gene ID" value="ENSSORG00005004371.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTD7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000005">
        <property type="entry name" value="Crystallin, beta B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000002">
        <property type="entry name" value="Crystallin, beta B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF55">
        <property type="entry name" value="BETA-CRYSTALLIN B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="7">
        <location>
            <begin position="55"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="7">
        <location>
            <begin position="95"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="7">
        <location>
            <begin position="187"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="244" mass="28437" checksum="9CACD30C05907A7D" modified="2020-06-17" version="1">MSAGGESKSKSASQTDGKASQNKMSEMGMMSYKMDCSSSFLFDHVVYSLLFRVKLQMCVYDQENFQGRCMEINNECMNVCDMGMDRVRSLRVDCGPFVGFEQMNFCGEMFILEKGEYPRWDSWSNCQKNDYLLSFRPVRMDPEKHKICLYEVGEFKGRKMEIMDDDVPSLFSYGFTDRVGSIMVSCGTWVGYQFPGYRGSQYLLEKGDYRHFNEFGARHPQMQSIRRIRDMQWHPHGCYTMSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTN5</accession>
    <name>A0A672YTN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hs3st1l2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A672YTN5"/>
    <dbReference type="FunCoup" id="A0A672YTN5">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008269.1">
        <property type="protein sequence ID" value="ENSSORP00005007986.1"/>
        <property type="gene ID" value="ENSSORG00005004470.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTN5"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002997">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF73">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025658396" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="23"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="8">
        <location>
            <begin position="58"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="65"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="271"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="35353" checksum="5623BFA2D0FBE85F" modified="2020-06-17" version="1">MLWTVLLALLVLLLLQTQLSVCIRELRTGGSSSPIYSSSSSSSSSSMFNATQQRLPGAIIIGVRKGGTRALLEMLNLHPNVEVAKAEVHFFNVEEHYRRGLAWYRTQMPFTLPGQVTVEKTPGYFAAPQAPARVWDMNPAVRLLLIVRDPAERLVSDYTQVLHNRLTRHKPYQPLEELLLHKGHIDPSYKALQRSLYHLHLDRWLAVFPREQIHVVDGDALIRDPFPELRKAERFLDLPPRISPNNFYYNTTKGFYCLLSAGHDKCLDESKGRPHAPLSPQAFKKLCRYFRKPNKLFFEMVGRSFSWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YW18</accession>
    <name>A0A672YW18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YW18"/>
    <dbReference type="Ensembl" id="ENSSORT00005009060.1">
        <property type="protein sequence ID" value="ENSSORP00005008759.1"/>
        <property type="gene ID" value="ENSSORG00005004837.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YW18"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="113"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="889" mass="100417" checksum="E0DD01B730A2B306" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWU5</accession>
    <name>A0A672YWU5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">EH-domain containing 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115432281</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YWU5"/>
    <dbReference type="Ensembl" id="ENSSORT00005009512.1">
        <property type="protein sequence ID" value="ENSSORP00005009206.1"/>
        <property type="gene ID" value="ENSSORG00005004615.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YWU5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000147">
        <property type="entry name" value="EH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000038">
        <property type="entry name" value="EH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040990">
        <property type="entry name" value="DUF5600"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF62">
        <property type="entry name" value="EH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18150">
        <property type="entry name" value="DUF5600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="54"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="12">
        <location>
            <begin position="448"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="480"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="544" mass="62890" checksum="772FD1770256EBD0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YY36</accession>
    <name>A0A672YY36_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YY36"/>
    <dbReference type="Ensembl" id="ENSSORT00005009838.1">
        <property type="protein sequence ID" value="ENSSORP00005009518.1"/>
        <property type="gene ID" value="ENSSORG00005005211.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YY36"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="136"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="336"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="166"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="234"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="536" mass="61459" checksum="16EC19DA4FD04FB5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYA3</accession>
    <name>A0A672YYA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NAD-dependent protein deacylase sirtuin-5, mitochondrial</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Regulatory protein SIR2 homolog 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">SIR2-like protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SIRT5</name>
        <name evidence="15" type="synonym">sirt5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-glutaryl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-glutaryl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87828"/>
            <dbReference type="ChEBI" id="CHEBI:87829"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-malonyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-malonyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11878"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87831"/>
            <dbReference type="ChEBI" id="CHEBI:87833"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
            <location evidence="2 12">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
        <text evidence="12">Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-101 and Arg-104) that bind to malonylated and succinylated substrates and define the specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A672YYA3"/>
    <dbReference type="FunCoup" id="A0A672YYA3">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009864.1">
        <property type="protein sequence ID" value="ENSSORP00005009545.1"/>
        <property type="gene ID" value="ENSSORG00005005225.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1600.10:FF:000005">
        <property type="entry name" value="NAD-dependent protein deacylase sirtuin-5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001753">
        <property type="entry name" value="PRK00481.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 12" id="KW-0520">NAD</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 12 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="14">
        <location>
            <begin position="32"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12 13">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="248"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="274"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="308" mass="34027" checksum="D1563137B30E3A24" modified="2020-06-17" version="1">QNLMIVSSLTRRGLTHSHLHAHLRKPRSSQDMARPSSDLAAFREIFSKSKNIAILTGAGVSAESGVPTFRGAGGYWRKWQAQDLATPEAFARNPSRVWEFYHYRREVMLTKNPNPAHLAIAECEERLSKQGRQVTVITQNIDELHRRAGSKNILEIHGSLFRTRCMNCGQEAANYKSPICAALEGKGAPDPNTDDAQLPVQQLPRCEQTGCQGLLRPAVVWFGETLDSDILTQVETVLDSCDLCLIVGTSSVVYPAAMFAPQVAERGVPVAEFNMEHTPATMRFKFHFHGPCGTTLPPALARHEAEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYM7</accession>
    <name>A0A672YYM7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane 6 superfamily member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tm6sf2b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TM6SF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YYM7"/>
    <dbReference type="Ensembl" id="ENSSORT00005010144.1">
        <property type="protein sequence ID" value="ENSSORP00005009816.1"/>
        <property type="gene ID" value="ENSSORG00005005366.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYM7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21106">
        <property type="entry name" value="TM6SF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047195">
        <property type="entry name" value="TM6SF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059044">
        <property type="entry name" value="TM_Tm6sf1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14568:SF13">
        <property type="entry name" value="SI:DKEY-19F23.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14568">
        <property type="entry name" value="TRANSMEMBRANE SUPERFAMILY 6 MEMBER 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26083">
        <property type="entry name" value="TM_Tm6sf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="108"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="214"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="268"/>
        </location>
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            <end position="300"/>
        </location>
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            <begin position="312"/>
            <end position="335"/>
        </location>
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    <feature type="domain" description="EXPERA" evidence="9">
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            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="197"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="366" mass="41631" checksum="F4A9D125A10CBFD7" modified="2020-06-17" version="1">METFVFIFSFSALGILYAMNTIPEFQEPYVILEIGVAVLVIVFLFYFLITRGNPPKDLLFFVYFKTNERISMCYTITGSGEPYLGTPYAIMMCYWDGIAHFIMYLVMISRITDRKAYRTIGLFWAGSLCANMSVFISGIVAGKYGSEIRPAFWLNFLFLLMPVWGAVTLFTRPKDRPLIGGYNAQHAQSMKLIWRPLDLILVVLLFGAIAFTILRGLVALDSPLEACSLYLRHYEPYLKDPVGYPRVMMLHLFFYGLPLLGAFVYGLLKPGCTWMTDWTIFFAGAMTQCQWAHIGASLHPRTASPFRIPGDAFWMVLAANLLYAVTPLLVAMRVYSNPYFFLKIAPFPGQTGLPNSEEKDTKYKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYV5</accession>
    <name>A0A672YYV5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">T-box transcription factor 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YYV5"/>
    <dbReference type="Ensembl" id="ENSSORT00005009896.1">
        <property type="protein sequence ID" value="ENSSORP00005009577.1"/>
        <property type="gene ID" value="ENSSORG00005005231.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYV5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20188">
        <property type="entry name" value="T-box_TBX2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000003">
        <property type="entry name" value="T-box transcription factor TBX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
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        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002070">
        <property type="entry name" value="TF_Brachyury"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF181">
        <property type="entry name" value="OPTOMOTOR-BLIND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00938">
        <property type="entry name" value="BRACHYURY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="86"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="389" mass="43242" checksum="B0403B3392E8867C" modified="2020-06-17" version="1">PAGTIAGRDQISTKLNPCRLVPHPFGGGGCEEAGGSSRGAHETAGRTSYDPAGGPTSTMEQHAILCRPPQILEGGEMKEDELKVHLESSELWRQFHTSGTEMVITKSGRRMFPPLKARCTGMDAKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMSKVVNFHKLKLTNNISDKHGYTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFSETEFIAVTAYQNDKITQLKIDNNPFAKGFRDTDTSTSTSTHGAEKEHLYKFHNSFAHGCFHPAELNRHLQDPSGLLQSAHISSGNAWYGCGTVDRPGPCGLSLATPIRTPGTLGFPMSLQQHALVQVRRGSTHPRMHVRSIAIIKANEHKTAQTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZB6</accession>
    <name>A0A672YZB6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleolus and neural progenitor protein-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nepro</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YZB6"/>
    <dbReference type="FunCoup" id="A0A672YZB6">
        <property type="interactions" value="1266"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010275.1">
        <property type="protein sequence ID" value="ENSSORP00005009945.1"/>
        <property type="gene ID" value="ENSSORG00005005424.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZB6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045747">
        <property type="term" value="P:positive regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Nepro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027951">
        <property type="entry name" value="Nepro_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34761">
        <property type="entry name" value="NUCLEOLUS AND NEURAL PROGENITOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34761:SF1">
        <property type="entry name" value="NUCLEOLUS AND NEURAL PROGENITOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14780">
        <property type="entry name" value="NEPRO_N"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolus and neural progenitor protein-like N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="364" mass="41929" checksum="629717BB0623328C" modified="2020-06-17" version="1">MKLDVALQDLSDLCPSGIQRSVSLKVCEASVPSQPMLEWVCLRVLGAAQLLNRTMSRCSRAFVLSKHHFKWEEFIVLNMVITSMLSRLWVVFRGVLVSLSTLYLELLHLRGVVAHARPMPFLTSFSLPVDLLDFLGPGGSVLIKRSLQSKKPEEKKRRRQKKEKHQQTRRVPEDLGVSVHRGLIKDSDMKPFLNIFRKFTEGKSIPHKAQKKQTFKEQMRGATSFTDAGIHLEKMIQWCRSQRMEREKRLLTFLRLKCQKMKSLEAAGYSVHRKLRTFRQEVHSVLSPQKSSPKTCGSVRSKRRSARLRSRIQTLRSEMKATTGGRRRQRRRKTEGDGRIRTRNEADPQITHCDIDDIFASAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0D5</accession>
    <name>A0A672Z0D5_9TELE</name>
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        <submittedName>
            <fullName evidence="13">Interleukin-6 receptor subunit beta-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115425752</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029999354.1">
        <property type="nucleotide sequence ID" value="XM_030143494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z0D5"/>
    <dbReference type="Ensembl" id="ENSSORT00005010522.1">
        <property type="protein sequence ID" value="ENSSORP00005010189.1"/>
        <property type="gene ID" value="ENSSORG00005005533.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425752"/>
    <dbReference type="InParanoid" id="A0A672Z0D5"/>
    <dbReference type="OrthoDB" id="9828391at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025585651" evidence="11">
        <location>
            <begin position="18"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="134"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="333"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="728" mass="81601" checksum="19DC58ACE0FB151D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2D6</accession>
    <name>A0A672Z2D6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein sel-1 homolog 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:dkey-24p1.6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z2D6"/>
    <dbReference type="FunCoup" id="A0A672Z2D6">
        <property type="interactions" value="426"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011143.1">
        <property type="protein sequence ID" value="ENSSORP00005010772.1"/>
        <property type="gene ID" value="ENSSORG00005005843.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2D6"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042756">
        <property type="entry name" value="Sel-1L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44444">
        <property type="entry name" value="PROTEIN SEL-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44444:SF4">
        <property type="entry name" value="PROTEIN SEL-1 HOMOLOG 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025496564" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="991"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1022" mass="116163" checksum="6ABABDB6F90C1D9B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z369</accession>
    <name>A0A672Z369_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z369"/>
    <dbReference type="Ensembl" id="ENSSORT00005011628.1">
        <property type="protein sequence ID" value="ENSSORP00005011250.1"/>
        <property type="gene ID" value="ENSSORG00005006039.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z369"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="100" mass="12225" checksum="4EDADD9EBC43717D" modified="2020-06-17" version="1">MDQVTVKWMNCYNWTVHVYEDGSDRTNEQNDEYRNRTEMNEDPLRTGDLSLTLKHLRDWDTHTYTCTVYNRHKQILRRKQVDLKVKGQSCRHRSFLVLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4N9</accession>
    <name>A0A672Z4N9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bifunctional lysine-specific demethylase and histidyl-hydroxylase</fullName>
            <ecNumber evidence="9">1.14.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">riox2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-histidyl-[protein] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-histidyl-[protein] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54256"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:13840"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138021"/>
            <dbReference type="EC" id="1.14.11.79"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-histidyl-[ribosomal protein uL15] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-histidyl-[ribosomal protein uL15] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54024"/>
            <dbReference type="Rhea" id="RHEA-COMP:13760"/>
            <dbReference type="Rhea" id="RHEA-COMP:13761"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138021"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ROX family. MINA53 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z4N9"/>
    <dbReference type="Ensembl" id="ENSSORT00005012196.1">
        <property type="protein sequence ID" value="ENSSORP00005011810.1"/>
        <property type="gene ID" value="ENSSORG00005006289.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z4N9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032453">
        <property type="term" value="F:histone H3K4 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036139">
        <property type="term" value="F:peptidyl-histidine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1500">
        <property type="entry name" value="JmjC domain-containing ribosomal oxygenase (ROX), dimer domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039994">
        <property type="entry name" value="NO66-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096">
        <property type="entry name" value="MINA53 MYC INDUCED NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096:SF7">
        <property type="entry name" value="RIBOSOMAL OXYGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08007">
        <property type="entry name" value="JmjC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="11">
        <location>
            <begin position="137"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="470" mass="53862" checksum="C7CBDC614B0B9898" modified="2020-06-17" version="1">MPKKSKGAPVKRPGSDEEQLHPKQSRIDSPLCFDSPASLFESLILPMRTEEFFRDYWEKKPLHLQRTDPQMASYYQSLFQVSDLQSLSSQGLEYYRDVNVVRCVSGKKKVLNKEGNVKSTVLHKNLVQNKATIQFHQPQRFKDELWRIQEKLECFFGALVGSNVYITPEESQGLPAHYDDVEVFILQLEGQKQWRLYNPTVPLAAEYSVESEDKIGSPTHSILLKAGDLLYFPRGTIHQANTPAGVDHSTHLTLSTYQRTSWGDLLLDTFPSVLSNRSKTDVSLREGMPRRFLLVRKIDINDDLDTRRKLASYLRCLADEMDGGKQEVHSTHMKRDFIINRLPPYCHQDLLTPSGRVPALEDTVCLKFKDHMLITIEPSPRTTDEATEQVVFVLHSLKNQRERHMMGASDDEEVEQDMSTGLQFPLSHFPALRQLQQVDQMPVNQLQLSTQEDKLSLVLALWSESLLTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4Q3</accession>
    <name>A0A672Z4Q3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">EF-hand calcium binding domain 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z4Q3"/>
    <dbReference type="Ensembl" id="ENSSORT00005012216.1">
        <property type="protein sequence ID" value="ENSSORP00005011830.1"/>
        <property type="gene ID" value="ENSSORG00005006293.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z4Q3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903569">
        <property type="term" value="P:positive regulation of protein localization to ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="369" mass="41976" checksum="A5DDAB0F940ECAE1" modified="2020-06-17" version="1">MSVLWLLLQEWQHSYMKGCFFLEDDGSICSLQYQLHVPQTTTVYLTIQPLSPSHRPGIGCHGDWSVDTALFVMSVGENKEDCTFVCFTESKDQEVRYVWKGELNAGTYYLLPFTSGCRLKKRSRKSPGNKPVELVYRTDTGELDLTRELREVLSDIFEVIDLDGNNLLSLEEYNFFELRTSGEKCDKEAWAVCKENFDMRKNQLTQQGFMELNLMEATEKDGDPADLWVTLEAMGYNRMLELVEACPFQIDVHCENTQPSILPLNLDSGPKLLNQAVQKSITTRTGAKALRGQDNIFIYTYKGEHRISSLIANKSNHKVMVHVNNEQNKNCCNSRGMNVFAVEVPARTKMGRQGVLCVCVCLFVCLLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z570</accession>
    <name>A0A672Z570_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Family with sequence similarity 219 member B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fam219b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM219 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979070.1">
        <property type="nucleotide sequence ID" value="XM_030123210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z570"/>
    <dbReference type="FunCoup" id="A0A672Z570">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012397.1">
        <property type="protein sequence ID" value="ENSSORP00005012005.1"/>
        <property type="gene ID" value="ENSSORG00005006366.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411211"/>
    <dbReference type="CTD" id="57184"/>
    <dbReference type="InParanoid" id="A0A672Z570"/>
    <dbReference type="OrthoDB" id="9451307at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029339">
        <property type="entry name" value="FAM219"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31281">
        <property type="entry name" value="PROTEIN FAM219A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31281:SF2">
        <property type="entry name" value="PROTEIN FAM219B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15260">
        <property type="entry name" value="FAM219A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="20391" checksum="302FDEA927523B79" modified="2020-06-17" version="1">MMNDILEEPEKDTLLETQPEPQVSTGPSIGTRPKTGDGGIRPVEKRGPYIMSRAPAIHLKLQKHREMARKALKKKALSPGPPVMHQPRQGAKRTVKYNKGYAALSQHAEETLVALDSDSDEEIDFEQYSSGYSSAEVHPDLSKQLLQDGYRLDEIPDDEDLDLIPPKAMGSSVCCCSEGPSCSMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9Y4</accession>
    <name>A0A672Z9Y4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF1279 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fam210aa</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z9Y4"/>
    <dbReference type="Ensembl" id="ENSSORT00005013717.1">
        <property type="protein sequence ID" value="ENSSORP00005013307.1"/>
        <property type="gene ID" value="ENSSORG00005006898.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9Y4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF1">
        <property type="entry name" value="PROTEIN FAM210A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF1279" evidence="7">
        <location>
            <begin position="76"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="225" mass="25521" checksum="61E00E387EC689AA" modified="2020-06-17" version="1">MTVYVCVLQSQSAVRMMRRLPFYWMRRRMTHLLAAQDCSGPCRPFQSSPEWHGPAQPSAGPPVDPLQDSSLGLGSRFKLALQQYGKVLIPVHLITSAAWFGSFYCAAMNGMDVVPLLEMVPGFPERIVDLLQNSPGGHALTAYTLYKVATPARYTISLGGTSLSVRYLRRHGYMTPPPPVREYLQDKMEETKEKLKGRKDMKDKGDKRQQYLEETNLKPIWLLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZA25</accession>
    <name>A0A672ZA25_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">microtubule-severing ATPase</fullName>
            <ecNumber evidence="11">5.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">spast</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.</text>
            <dbReference type="EC" id="5.6.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.1" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZA25"/>
    <dbReference type="FunCoup" id="A0A672ZA25">
        <property type="interactions" value="1399"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013778.1">
        <property type="protein sequence ID" value="ENSSORP00005013368.1"/>
        <property type="gene ID" value="ENSSORG00005004692.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZA25"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02679">
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    <sequence length="541" mass="59944" checksum="816145F4CAE314A2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAC9</accession>
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        <submittedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Neoteleostei</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="290" mass="32410" checksum="8D12B3973ABC3DD2" modified="2020-06-17" version="1">MYQRLIKVGLAKYDPFKTRFQINLSTFILTFNPFSPIGPPLFAIFAFATCGGYSGQLRVSVDCMEKASSNLSIGIDFAYPFRLYQVSFEAPACEAMRRDRVYLIGDYSSSAEFFVTIAVFAFLYSLVATGVYVFFLNRYREISRGPLIDFVVTVVFSFMWLVSSSAWAKALSDVKLATDPDEVQLLISACKEPTNKCGSVHGPRWSGLNTSVVFGFLNFVLWAGNIWFVFKETSWHKGASKFPNAASEKQTGAFNQQPYNQGTFDQSGSYNTQPSEYSQVGGPTSYSNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZB50</accession>
    <name>A0A672ZB50_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">PBZ-type domain-containing protein</fullName>
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        <name evidence="3" type="primary">aplf</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSSORG00005007238.1"/>
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        <property type="component" value="Chromosome 1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="APLF"/>
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        <property type="entry name" value="APLF_PBZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="APRATAXIN AND PNK-LIKE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21315">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SMAD/FHA domain"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
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            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
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        <location>
            <begin position="108"/>
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        </location>
    </feature>
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        <location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
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        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
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        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
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            <begin position="363"/>
            <end position="375"/>
        </location>
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            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
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        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="471" mass="52339" checksum="BA5C9FAE1F7D7A7F" modified="2020-06-17" version="1">MSRFDLVPVDGGEPIHLPLGDTVLGRGPLLGVSDKRVSRHHGLLENLNGQLRLKPTHLNPCFVQSSLSDDPRPLQKDTWFPLGDGDLFSLLPGQFIYRVVAVGGEEATPRNSQMFEEEENKSPIPPSPDVEPAPSPVGLKCGQTLPHEGTTQAALSDEANRPLHQAASVFLKDEEAQTGVTPPTVYRKRILPGWMMAATSNGRSSSSKAVKRSKGPVSSSAATQRPAAKQTTPPLATLPKGVGVHEEEKEEEEKRPKKRRRKVSDEEVQATTCKEERDDLPVEEEEEEDRGRTLTANRNATNVEKKPENTGETSQRVKPVGSNSSSVSGTASSKRLPCPYGKDCYRKNPIHFQECSHPGDPDYKEEEEDEEEEADRPECPYGTDCYRKNPLHKKEFKHTKRPARTSRTVAQKPQADDDEDDDGEDDDSFINDDSEDLGDDSDYMPPDSDDSGKEEIKRLQKEAKVFLKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBY0</accession>
    <name>A0A672ZBY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBC1 domain family member 20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZBY0"/>
    <dbReference type="Ensembl" id="ENSSORT00005014418.1">
        <property type="protein sequence ID" value="ENSSORP00005013998.1"/>
        <property type="gene ID" value="ENSSORG00005007175.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZBY0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000024">
        <property type="entry name" value="TBC1 domain family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1310:FF:000001">
        <property type="entry name" value="TBC1 domain family, member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF11">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="7">
        <location>
            <begin position="60"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="388" mass="44812" checksum="C83DE818806227DF" modified="2020-06-17" version="1">MKRVKRKKPNAAVVVNENGRISREPDCGKKHKLAEIHQALNSDPVDIGTLRRAAGSKGGLLTEELRRKVWPKLLNINVYNLPHKPCQDVRKNHKDYNQVVLDVRRSTKRFPKGMPARDRAVLQEQLIDVILEVLKCNPQLHYYQGYHDVAVTLLLVVGKRMAVAMLHTLSNYHLRDFMDPTMDSTKHILNYLMPILEQVDTELHDFMIRAEVGTIFALSWLITWYGHVLSDFKHILRLYDFFLSSHPLMPIYFAATIVLHREKEVKQTECDMAMVHHLLSRIPDDLPYEHLITQSQHLFDLYPPSLLAKQASLQSRKSLSISTFQAFHLSTLHQRPDSVLQRLTKAKGRHGLEAALSQDQGQLWGRGNRMVKMAVWGCRRPWEPPCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZD42</accession>
    <name>A0A672ZD42_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZD42"/>
    <dbReference type="Ensembl" id="ENSSORT00005014800.1">
        <property type="protein sequence ID" value="ENSSORP00005014377.1"/>
        <property type="gene ID" value="ENSSORG00005007363.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZD42"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="82" mass="9134" checksum="431E21835467E372" modified="2020-06-17" version="1">LLMAKWLPTLLHDKEIPPEMFSTRHSGGGSIMIWGFQTVDALREAIFTTWSNVPTSLLETLASSMPKQIFEVISKNGGATHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZEN8</accession>
    <name>A0A672ZEN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mettl16</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the methyltransferase superfamily. METTL16/RlmF family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZEN8"/>
    <dbReference type="FunCoup" id="A0A672ZEN8">
        <property type="interactions" value="2185"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015387.1">
        <property type="protein sequence ID" value="ENSSORP00005014953.1"/>
        <property type="gene ID" value="ENSSORG00005007602.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZEN8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120048">
        <property type="term" value="F:U6 snRNA (adenine-(43)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009896">
        <property type="term" value="P:positive regulation of catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051254">
        <property type="term" value="P:positive regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000062">
        <property type="entry name" value="U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017182">
        <property type="entry name" value="METTL16/PsiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393:SF0">
        <property type="entry name" value="RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037350">
        <property type="entry name" value="Mtase_ZK1128_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="377"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="420"/>
            <end position="450"/>
        </location>
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</entry>
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</entry>
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    <accession>A0A672ZGE4</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="Ensembl" id="ENSSORP00005015537.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="85" mass="9864" checksum="D3FB8E42E3F43CB0" modified="2020-06-17" version="1">MMEKSNSQYDSFLFWRQPIPALDLSELEDLGLIKEPINRSKGKDKKRSQDEEVRKSELSEFSSFNYWRAPLVSVDALLADLSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQD6</accession>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neurexophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZQD6"/>
    <dbReference type="Ensembl" id="ENSSORT00005019615.1">
        <property type="protein sequence ID" value="ENSSORP00005019059.1"/>
        <property type="gene ID" value="ENSSORG00005009387.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQD6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010450">
        <property type="entry name" value="Nxph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103">
        <property type="entry name" value="NEUREXOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103:SF10">
        <property type="entry name" value="NEUREXOPHILIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="242" mass="27461" checksum="786CF9FCD4584713" modified="2020-06-17" version="1">PGSCPPPSNVLLSTFPYGTVGGKTGGVVKNPYQTRVFSTSIDQTPMKTKPPTYSFFNPYDSARNQSLLLDQTGYRSKRKPSLKTAMKTKKIFGWGDFYFNVKTMKFSLLVTGKIVDHINGTFTVYFRHNSSSLGNVSVSIVPPTKVVEFEETQQSTIDPKEVKTFNCRVEYEKTNRSKKPKPCLYDPSQTCFTEHTQSHAAWLCAKPFKVICIFISFFSIDYKLVQKVCPDYNFQSEHPYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQF7</accession>
    <name>A0A672ZQF7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transcription factor 7 like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tcf7l2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZQF7"/>
    <dbReference type="Ensembl" id="ENSSORT00005019635.1">
        <property type="protein sequence ID" value="ENSSORP00005019079.1"/>
        <property type="gene ID" value="ENSSORG00005009376.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQF7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071664">
        <property type="term" value="C:catenin-TCF7L2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010456">
        <property type="term" value="P:cell proliferation in dorsal spinal cord"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060847">
        <property type="term" value="P:endothelial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021903">
        <property type="term" value="P:rostrocaudal neural tube patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21996">
        <property type="entry name" value="HMG-box_TCF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000001">
        <property type="entry name" value="transcription factor 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000002">
        <property type="entry name" value="transcription factor 7-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013558">
        <property type="entry name" value="CTNNB1-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF32">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01366">
        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="HMG box" evidence="12">
        <location>
            <begin position="328"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="10">
        <location>
            <begin position="328"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="296"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="480"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="551"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="551"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="617" mass="67896" checksum="ECCB6347DA5D803E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZRB4</accession>
    <name>A0A672ZRB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">kifc3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZRB4"/>
    <dbReference type="Ensembl" id="ENSSORT00005020392.1">
        <property type="protein sequence ID" value="ENSSORP00005019830.1"/>
        <property type="gene ID" value="ENSSORG00005009145.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZRB4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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            <begin position="866"/>
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        <location>
            <begin position="585"/>
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        <ligand>
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            <dbReference type="ARBA" id="ARBA00004284"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <sequence length="913" mass="102691" checksum="26E8767F002CBE9D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <sequence length="422" mass="45503" checksum="334169004C57AEDA" modified="2020-06-17" version="1">MLGPLLGFDKNIVLMMTRQQVLTLMSMASVNFSSMICYSILGPFFPNEAIKKGASQTTVGLIFGCYAVCNLIGSLVLGKYIVQIGAKFMLVAGLFVSSGCTIMFGLLDRAPAGPAFITLCFIVRSVDAVGFAAAMTSSFALTAKIFPNNVATVLGSLEIFTGLGLILGPPVGGWFYQSFGYEVPFMLLGCFLLVMVPFNIYILPSIDAVPSKDSFFRLLTHFKIVLICYAIFTLSAGLGFLDATLSLFAIEKFKLTPGYVGLIMLGLSLPYCLASPLMGYFTDKYPATRRWFMVMGGIITALGFCLLGPAPFLHIPSQLWLLVLMLGLIGFSLGMTAIPTFPEIITCAYQNGFEEGLSTLGMVSGLFGAVWSMGMFYGPIVGGLITQHLSFEWAAVVQAGLGFLAVSIIYNHQLDETRTSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZYD2</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="11" type="primary">arhgef4</name>
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            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 4 ISOFORM X1"/>
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    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="31"/>
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            <end position="102"/>
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            <begin position="141"/>
            <end position="325"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="531" mass="62123" checksum="04383A97FC0E559C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2K8</accession>
    <name>A0A673A2K8_9TELE</name>
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        <recommendedName>
            <fullName evidence="5">TBC1 domain family member 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="34" type="primary">zhx2a</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="26">Molecular adapter which is involved in cilium biogenesis. Part of a functional complex including OFD1 a centriolar protein involved in cilium assembly. Could regulate the cAMP-dependent phosphorylation of OFD1, and its subsequent ubiquitination by PJA2 which ultimately leads to its proteasomal degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="3">Centrosome</location>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 27 29">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZHX family.</text>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="Pfam" id="PF18387">
        <property type="entry name" value="zf_C2H2_ZHX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="4"/>
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        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="25" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="19 30" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-0221">Differentiation</keyword>
    <keyword evidence="20 27 29" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="21 27 29" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24 27 29" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="22" id="KW-0804">Transcription</keyword>
    <keyword evidence="18" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 28" id="KW-0853">WD repeat</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Homeobox" evidence="32">
        <location>
            <begin position="227"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="32">
        <location>
            <begin position="406"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="32">
        <location>
            <begin position="483"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="28">
        <location>
            <begin position="1010"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="33">
        <location>
            <begin position="1119"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="27">
        <location>
            <begin position="229"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="27">
        <location>
            <begin position="408"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="27">
        <location>
            <begin position="485"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="533"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="30">
        <location>
            <begin position="1414"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="31">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="495"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="31">
        <location>
            <begin position="545"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00034464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1638" mass="185006" checksum="164DF75709657628" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5L8</accession>
    <name>A0A673A5L8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Reticulon 4 receptor-like 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">rtn4rl2b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the Nogo receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A5L8"/>
    <dbReference type="Ensembl" id="ENSSORT00005025265.1">
        <property type="protein sequence ID" value="ENSSORP00005024551.1"/>
        <property type="gene ID" value="ENSSORG00005011802.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A5L8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905573">
        <property type="term" value="F:ganglioside GM1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905576">
        <property type="term" value="F:ganglioside GT1b binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000018">
        <property type="entry name" value="Reticulon 4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF174">
        <property type="entry name" value="RETICULON-4 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025653862" evidence="16">
        <location>
            <begin position="33"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="17">
        <location>
            <begin position="32"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="18">
        <location>
            <begin position="263"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="454" mass="51865" checksum="01EE303E3B0E11C4" modified="2020-06-17" version="1">MDLTDTQRKGWCGLLLWLILWLVVMKPGGVTACPKLCVCYPTPMTVSCQSQNLTIIPTGVPYDSQRVFLQNNRITELRADSFGFETQVLWLYGNNITWIEAGAFSNLRVLEELDLGDNPSLRHLEGGAFRGLEKLQSLHMHRCKLATLPHDLFHKLYSLQFLYLQENQLHFLHDDLFSDLVNLTHLFLHGNRIRTLSENVFRGLVNLDRLLLHDNRIRQVNRRAFRDLGRLTILYLFNNSLAELPGQTMKDVQGIQFLRLNSNPWSCGCEARPLWEWFREARISSSELMCTSPSQRRGQDLRFLRELDFALCPLPDPGSLAGSTTTTFSTKTRWWFSKHKPASSSKALYQKSSETVKAFPFTAPKPQFPPKVPSESIPSKYELSEDEAALPKLDPEEYWANYGNEDSSIRCFELECPPGYDSPYPSSSSLSLTPSFLHLLSLSVVIFSLHLLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5M6</accession>
    <name>A0A673A5M6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBX domain-containing protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UBX domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ubxn6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May negatively regulate the ATPase activity of VCP, an ATP-driven segregase that associates with different cofactors to control a wide variety of cellular processes. As a cofactor of VCP, it may play a role in the transport of CAV1 to lysosomes for degradation. It may also play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Together with VCP and other cofactors, it may play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with VCP through the PUB domain (via C-terminus) and VIM motif (via N-terminus); the interaction is direct. Forms a ternary complex with CAV1 and VCP. Interacts with SYVN1. Interacts with HERPUD1. Interacts with VCPKMT. May interact with DERL1. Interacts with PLAA, VCP and YOD1; may form a complex involved in macroautophagy. Interacts with LMAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029988174.1">
        <property type="nucleotide sequence ID" value="XM_030132314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A5M6"/>
    <dbReference type="FunCoup" id="A0A673A5M6">
        <property type="interactions" value="1293"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024677.1">
        <property type="protein sequence ID" value="ENSSORP00005023968.1"/>
        <property type="gene ID" value="ENSSORG00005011580.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418027"/>
    <dbReference type="CTD" id="80700"/>
    <dbReference type="InParanoid" id="A0A673A5M6"/>
    <dbReference type="OrthoDB" id="49605at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10460">
        <property type="entry name" value="PUB_UBXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16119">
        <property type="entry name" value="UBX_UBXN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000185">
        <property type="entry name" value="UBX domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042774">
        <property type="entry name" value="UBXN6_PUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153:SF38">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153">
        <property type="entry name" value="UBX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBX" evidence="10">
        <location>
            <begin position="327"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00059509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="436" mass="49315" checksum="BF7CEF99CA3F64C6" modified="2020-06-17" version="1">MKKFFEDIKKDIKFKSAGPGKKLTEDTSSKPEPVQSSSTVKNRHVPSQGAQMAGAAALARIEQQQRPKVHTSHDAIRNQVKRELEAEAAALAEKEKAAAAEGSKVKDPSCLSVSGVYFICPLTGAILTKSDREVHIKEAILMRFEEDAIEASVMMIHTFNKDRDKVKAAVDIISKYVENVCKNPTEEKYRKIKVSNKVFQEKVRCVEGTREFLEALGFISAMLPVEGQEEDEEFLVLPEQSPDALELMKERRDRLQRGEPVRAQLDRQPQAFRSSPNAQRFELPPEFYNLTAEELKKEQQQRSDLVEKNSMLRTKAMREREEQRERRKYNYTLLRIRLPDGNLLQGTFYAWDRLPVLFTFVRESLVDGWQPFELIAPGGQKLQQSEEVALAECNLVPAALLTFAWDAAVQADIAAAGGKSPSLLKPELLEKIQTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A861</accession>
    <name>A0A673A861_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dihydropyrimidinase like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dpysl3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A861"/>
    <dbReference type="FunCoup" id="A0A673A861">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026236.1">
        <property type="protein sequence ID" value="ENSSORP00005025476.1"/>
        <property type="gene ID" value="ENSSORG00005006527.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A861"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016812">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.40.10:FF:000021">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000174">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF57">
        <property type="entry name" value="DIHYDROPYRIMIDINASE-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="8">
        <location>
            <begin position="184"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="625"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="626"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="697" mass="75842" checksum="256BEE9DC4428DE6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AAH6</accession>
    <name>A0A673AAH6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Hydroperoxide isomerase ALOXE3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115415144</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984478.1">
        <property type="nucleotide sequence ID" value="XM_030128618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AAH6"/>
    <dbReference type="Ensembl" id="ENSSORT00005026335.1">
        <property type="protein sequence ID" value="ENSSORP00005025572.1"/>
        <property type="gene ID" value="ENSSORG00005012299.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415144"/>
    <dbReference type="InParanoid" id="A0A673AAH6"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="13">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="14">
        <location>
            <begin position="120"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="545"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="668"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="11">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="668" mass="76710" checksum="3434A20FC0D148A4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABP3</accession>
    <name>A0A673ABP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Arrestin domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115430313</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors. Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation. May recruit NEDD4 to ADRB2. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ABP3"/>
    <dbReference type="Ensembl" id="ENSSORT00005027388.1">
        <property type="protein sequence ID" value="ENSSORP00005026609.1"/>
        <property type="gene ID" value="ENSSORG00005012741.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABP3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000007">
        <property type="entry name" value="Arrestin domain-containing protein 3 mRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF49">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="14">
        <location>
            <begin position="187"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="411" mass="45585" checksum="22346F592D69538F" modified="2020-06-17" version="1">MVLGKVRALAIYFDSLNENNLPAYSGGDLVSGRVVVEVTGDVRVKSLDITARGVAKVRWTESRNAGANTAYTQNYTEEVEYLHHYDTLIGEERDEDCPEEGLTVLHAGLHEFAFSFNLPQMALATSFEGKHGSVRYWVKAELHRPWLLPVKVKKEFIVFEHIDINTPLLLAPQAGTKEKTLCCWFCASGPISLSAKIERKGYTPGESIQIFAEVENCSSRVVVPKAALYQTQTFFAKGKGKQIEQLVSNLRGDPLPQGKSQSWEGKLLKIPPVSPSILDCPIIRVEYALVVYVDVPGGLNLSLSLPLVIGTIPLHACATRTSSISSNCSTLNWLGLTERPEAPPSYSDLAISESHRRDCLQGCDRSDEEGEEQGSVLTYITEFRYLPPPLYSEVQFIPTHTHTHADTLYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABY3</accession>
    <name>A0A673ABY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">All-trans-retinol 13,14-reductase</fullName>
            <ecNumber evidence="15">1.3.99.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">retsat.2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2</text>
            <dbReference type="Rhea" id="RHEA:19193"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:52075"/>
            <dbReference type="EC" id="1.3.99.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.23" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A673ABY3"/>
    <dbReference type="FunCoup" id="A0A673ABY3">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027870.1">
        <property type="protein sequence ID" value="ENSSORP00005027085.1"/>
        <property type="gene ID" value="ENSSORG00005012954.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABY3"/>
    <dbReference type="OrthoDB" id="38045at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051786">
        <property type="term" value="F:all-trans-retinol 13,14-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000362">
        <property type="entry name" value="All-trans-retinol 13,14-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052206">
        <property type="entry name" value="Retinol_saturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091:SF1">
        <property type="entry name" value="ALL-TRANS-RETINOL 13,14-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091">
        <property type="entry name" value="BLR7054 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
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    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="416" mass="45728" checksum="DB2D56964EB4A11B" modified="2020-06-17" version="1">MSTTSLDKELQERLREASAIGDIDEVRTLVESGVNVNSQNEINGWTCLHWACKRNHKHIVSYLLSCGADKEILTAKDELASQLTSKPEIKRLLGVEVEEVPEVKEPELPIIPNYLSNPPFFYSKMDKSEIALAQHLAQNGSGEHAEDTHSDSAPLSPTHEPQKMQNLVSDGPASTPNPSSHSQGQTGEFIPVSEQNGVSASPPSSHNHAVVNCTVPMDLSVEPHLINHADYPHAVAHNGTMCSPPLASPSPSLASNSGSQVQAPVPGANPTVSRQQSIPQQLSYSQASGSLPAFQPFFFTSTFPVNVQELVLKVRIQNPNARENDFIEVELDRQELTYRSLLRVCCRELDISAEHVEKIRKLPNTMLRKDKDVARLQDFQELEVVLEKAEGLSLFSGTGGLTDRPCYNMKASRLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APC2</accession>
    <name>A0A673APC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spermatogenesis-associated protein 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673APC2"/>
    <dbReference type="Ensembl" id="ENSSORT00005032399.1">
        <property type="protein sequence ID" value="ENSSORP00005031520.1"/>
        <property type="gene ID" value="ENSSORG00005015005.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APC2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120212">
        <property type="term" value="C:sperm head-tail coupling apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032027">
        <property type="term" value="F:myosin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042769">
        <property type="entry name" value="SPATA6_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032732">
        <property type="entry name" value="SPATA6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435:SF6">
        <property type="entry name" value="IP09370P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6 SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14909">
        <property type="entry name" value="SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 6 N-terminal" evidence="3">
        <location>
            <begin position="15"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="95" mass="10992" checksum="319D6EBD39DA3ADA" modified="2020-06-17" version="1">VKNISSLRCSRETRETVSCPGVHLPAKDDIYLSMCFMGQYRRSECLPAVFPLLFHEKMTFEKVFRYAVDPGDIAVMLECKLISVFFMRQEFSSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APD8</accession>
    <name>A0A673APD8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mast4</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="Ensembl" id="ENSSORT00005031287.1">
        <property type="protein sequence ID" value="ENSSORP00005030433.1"/>
        <property type="gene ID" value="ENSSORG00005013988.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APD8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05609">
        <property type="entry name" value="STKc_MAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000012">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000012">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1480.20:FF:000001">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000008">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1480.20">
        <property type="entry name" value="MAST3 pre-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037711">
        <property type="entry name" value="MAST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015022">
        <property type="entry name" value="MAST_pre-PK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023142">
        <property type="entry name" value="MAST_pre-PK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF224">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED SERINE_THREONINE-PROTEIN KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08926">
        <property type="entry name" value="DUF1908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140482">
        <property type="entry name" value="MAST3 pre-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="466"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="18">
        <location>
            <begin position="714"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="17">
        <location>
            <begin position="995"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="121"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="790"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="846"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="966"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1088"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1404"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1684"/>
            <end position="1803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2048"/>
            <end position="2067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2073"/>
            <end position="2094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2121"/>
            <end position="2450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="790"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="804"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="875"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="906"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="966"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1095"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1135"/>
            <end position="1155"/>
        </location>
    </feature>
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        <location>
            <begin position="1176"/>
            <end position="1199"/>
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    </feature>
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        <location>
            <begin position="1246"/>
            <end position="1256"/>
        </location>
    </feature>
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        <location>
            <begin position="1300"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1404"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1716"/>
            <end position="1730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1731"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1748"/>
            <end position="1768"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="2053"/>
            <end position="2067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2140"/>
            <end position="2151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2185"/>
            <end position="2204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2353"/>
            <end position="2377"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2390"/>
            <end position="2403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2427"/>
            <end position="2443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2450" mass="267685" checksum="2CA23DD1DFFDA973" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQI3</accession>
    <name>A0A673AQI3_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">capn10</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
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    </dbReference>
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            <end position="321"/>
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        <location>
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        <location>
            <position position="267"/>
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    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="672" mass="76189" checksum="46F6C46042E7ADC6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARM4</accession>
    <name>A0A673ARM4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ARM4"/>
    <dbReference type="Ensembl" id="ENSSORT00005031894.1">
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        <property type="gene ID" value="ENSSORG00005014802.1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="216" mass="24411" checksum="EB8BA2092E61416F" modified="2020-06-17" version="1">MRKGHSSKQRPGTRRPRDTRSLRDIDGPGRPLYDPPDLLETAAIEVWLRRDQAGYQRRPQGGDQRRLQGGDQRRPQGGDQRRLQGGDQRRPQGGDQRRPQGGDQRRLQGGDQRRPQGGDQRRPQGGDQRRPQGGDQRRLQGGDQRRPQGGDQRRPQGGDQRRLQGGDQRRPQGGDQRRPQGGDQRRPQGGDQEPRHPGTGLSNIRPVDLWVLFGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AW70</accession>
    <name>A0A673AW70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the globin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AW70"/>
    <dbReference type="Ensembl" id="ENSSORT00005034818.1">
        <property type="protein sequence ID" value="ENSSORP00005033905.1"/>
        <property type="gene ID" value="ENSSORG00005016040.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AW70"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031838">
        <property type="term" value="C:haptoglobin-hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005833">
        <property type="term" value="C:hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031720">
        <property type="term" value="F:haptoglobin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:organic acid binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:oxygen binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050056">
        <property type="entry name" value="Hemoglobin_oxygen_transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442">
        <property type="entry name" value="HEMOGLOBIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442:SF7">
        <property type="entry name" value="HEMOGLOBIN SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="7">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="132" mass="14925" checksum="11E4F5ED7CF14035" modified="2020-06-17" version="1">MVEWSDQERSIITSIFDHVLTHLFSCRCLIVYPWTQRYFGCPRNQGAARSGSFECHALEKLHIDPDNFKISVCSIFDPEYSCIVFGLSHSCWLTALTIVVAAKMGTAFTADIQAAFQKFLSVVVNALGRQYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AY65</accession>
    <name>A0A673AY65_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">[histone-H3]-N(6),N(6)-dimethyl-L-lysine(4) FAD-dependent demethylase</fullName>
            <ecNumber evidence="24">1.14.99.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Flavin-containing amine oxidase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">kdm1b</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + 2 A + 2 H2O = L-lysyl(4)-[histone H3] + 2 formaldehyde + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:60244"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.99.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:60245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + A + H2O = L-lysyl(4)-[histone H3] + formaldehyde + AH2</text>
            <dbReference type="Rhea" id="RHEA:60256"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:60257"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="23">Interacts with its cofactor GLYR1 at nucleosomes; this interaction stimulates H3K4me1 and H3K4me2 demethylation. In contrast to KDM1A, does not form a complex with RCOR1/CoREST. Possible accessory component of the polycomb repressive deubiquitinase (PR-DUB) complex, at least composed of BAP1, one of ASXL1, ASXL2 or (probably) ASXL3 and one of MBD5 or MBD6. The PR-DUB core associates with a number of accessory proteins, including FOXK1, FOXK2, KDM1B, HCFC1 and OGT; KDM1B specifically associates with ASXL2 PR-DUB complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.66" evidence="24"/>
    <dbReference type="RefSeq" id="XP_030003554.1">
        <property type="nucleotide sequence ID" value="XM_030147694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AY65"/>
    <dbReference type="Ensembl" id="ENSSORT00005035070.1">
        <property type="protein sequence ID" value="ENSSORP00005034154.1"/>
        <property type="gene ID" value="ENSSORG00005014881.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428573"/>
    <dbReference type="CTD" id="221656"/>
    <dbReference type="InParanoid" id="A0A673AY65"/>
    <dbReference type="OrthoDB" id="2219495at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140682">
        <property type="term" value="F:FAD-dependent H3K4me/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000232">
        <property type="entry name" value="lysine-specific histone demethylase 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.100:FF:000002">
        <property type="entry name" value="Lysine-specific histone demethylase 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07496">
        <property type="entry name" value="zf-CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0678">Repressor</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CW-type" evidence="28">
        <location>
            <begin position="155"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="27">
        <location>
            <begin position="290"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00053130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00065941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00066706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00083218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="834" mass="94108" checksum="9F29116960300C56" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZA4</accession>
    <name>A0A673AZA4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Decorin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Bone proteoglycan II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">dcn</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">May affect the rate of fibrils formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Binds to type I and type II collagen, fibronectin and TGF-beta. Forms a ternary complex with MFAP2 and ELN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 14">Secreted</location>
            <location evidence="1 12 14">Extracellular space</location>
            <location evidence="1 12 14">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12 14">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030006157.1">
        <property type="nucleotide sequence ID" value="XM_030150297.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030006158.1">
        <property type="nucleotide sequence ID" value="XM_030150298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AZA4"/>
    <dbReference type="Ensembl" id="ENSSORT00005036136.1">
        <property type="protein sequence ID" value="ENSSORP00005035201.1"/>
        <property type="gene ID" value="ENSSORG00005016608.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430339"/>
    <dbReference type="CTD" id="1634"/>
    <dbReference type="InParanoid" id="A0A673AZA4"/>
    <dbReference type="OrthoDB" id="1111193at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000038">
        <property type="entry name" value="Biglycan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016352">
        <property type="entry name" value="SLRP_I_decor/aspor/byglycan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF14">
        <property type="entry name" value="DECORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002490">
        <property type="entry name" value="SLRP_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12 14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025710869" description="Decorin" evidence="12 14">
        <location>
            <begin position="17"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="15">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="53"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002490-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="39732" checksum="95B757141BDE3E35" modified="2020-06-17" version="1">MRSACLSLLLVTACWALPFRQSGFLDFMMEDEVGSGDPTTDISIPPMAEGPKCPFRCQCHLRVIQCSDLGLKAVPGDIPDDTTLLDLQNNKITEIKENDFKNLKGLHALILVNNKITVIHAKALNPLTKLQRLYLSKNMLKDVPANMPKSLQELRIHENEITKIKKSSFQGMSHIIVMELGSNPLKSAGVEAGAFGDLKRVSYIRIADTNISEIPKGLPSSLSELHLDGNKITKVTAESLKGLKNLAKLGLSYNEISSVENGSLANAPHLRELHLDNNALTSVPPGLPDHKYIQVVYLHSNKIAAVGTEDFCPPGFNTKKAMYSGISLFSNPVPYWEVQPITFRCVFDRSAIQLGNYRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZG5</accession>
    <name>A0A673AZG5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cornichon family AMPA receptor auxiliary protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cnih2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AZG5"/>
    <dbReference type="FunCoup" id="A0A673AZG5">
        <property type="interactions" value="458"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034581.1">
        <property type="protein sequence ID" value="ENSSORP00005033668.1"/>
        <property type="gene ID" value="ENSSORG00005015940.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZG5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033466">
        <property type="entry name" value="Cornichon_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01340">
        <property type="entry name" value="CORNICHON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="152" mass="18208" checksum="D6FF0ACF9DFC520C" modified="2020-06-17" version="1">MAFTFAAFCYMLTLVLCAALIFFVIWQIIAFDELRTDFKNPIDQSNPTRARERILNIERICNLLRRLVVPEYSIHGLFCLMFMCAGEWVTLGLNIPLLFYHLWRFFHRPRDGSEVMYDPVSVMNADILNYCQKESWCKMGFYLLSFFYYLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B117</accession>
    <name>A0A673B117_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cell surface glycoprotein CD200 receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">si:ch211-214p13.9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD200R family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B117"/>
    <dbReference type="Ensembl" id="ENSSORT00005036475.1">
        <property type="protein sequence ID" value="ENSSORP00005035525.1"/>
        <property type="gene ID" value="ENSSORG00005016768.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B117"/>
    <dbReference type="OrthoDB" id="8915654at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150077">
        <property type="term" value="P:regulation of neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040012">
        <property type="entry name" value="CD200R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21462:SF2">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN CD200 RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21462">
        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN OX2 RECEPTOR PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025663662" evidence="10">
        <location>
            <begin position="21"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="45"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="152"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="316" mass="35372" checksum="CA086BA311B0547B" modified="2020-06-17" version="1">MKEMKLVYAAIIFLVSEAWSLDQAFSRTNQSTSEMKLNATSSSSPNYVFKYSSLKLGRDANLTCGDKTWTEMMFVIWKLRLKNKDCDIAFHIDGQNRNTCNDGKSLQNTSEAQSFLFIPNFSFSDVGIYKCESVFSGGAEAYSINVSITVPPKLSSWLDWKGNKMVAVCKAEGGIPAANISWSHTGNTSTVETRDSSDGFFTVESRLELPTDMQTENVSCAISHLSWEHEESLMIKIEKAGRAFSWVHLAIPISIAFFLGFLFFGQKKLVKLRRGQESVVSSSKSPQTDDVEEVEPYASYVQRVNSIYNSSLDMFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2I4</accession>
    <name>A0A673B2I4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ap2s1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). Interacts with CCDC32; the interaction is direct and mediates association of CCDC32 with adaptor protein complex 2 (AP-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B2I4"/>
    <dbReference type="FunCoup" id="A0A673B2I4">
        <property type="interactions" value="1891"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035796.1">
        <property type="protein sequence ID" value="ENSSORP00005034872.1"/>
        <property type="gene ID" value="ENSSORG00005016419.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2I4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14833">
        <property type="entry name" value="AP2_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000004">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027156">
        <property type="entry name" value="APS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="12">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="152" mass="18129" checksum="CC3516AE7A37D8B5" modified="2020-06-17" version="1">MQIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEVWALSSLLWEKNFKIIYRRYAGLYFCICVDVTDNNLAYLEGIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2J1</accession>
    <name>A0A673B2J1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein wntless homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115417706</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the wntless family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B2J1"/>
    <dbReference type="Ensembl" id="ENSSORT00005037367.1">
        <property type="protein sequence ID" value="ENSSORP00005036406.1"/>
        <property type="gene ID" value="ENSSORG00005017159.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2J1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061355">
        <property type="term" value="P:Wnt protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047843">
        <property type="entry name" value="WLS-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053936">
        <property type="entry name" value="WLS_GOLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009551">
        <property type="entry name" value="Wntless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449:SF2">
        <property type="entry name" value="PROTEIN WNTLESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06664">
        <property type="entry name" value="WLS-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21883">
        <property type="entry name" value="WLS_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless GOLD" evidence="11">
        <location>
            <begin position="48"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless-like transmembrane" evidence="10">
        <location>
            <begin position="222"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="528" mass="60685" checksum="ADFE4EDF3F6B2412" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B545</accession>
    <name>A0A673B545_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Bone morphogenetic protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">bmp16</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B545"/>
    <dbReference type="Ensembl" id="ENSSORT00005036742.1">
        <property type="protein sequence ID" value="ENSSORP00005035787.1"/>
        <property type="gene ID" value="ENSSORG00005016889.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B545"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000103">
        <property type="entry name" value="Bone morphogenetic protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF246">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
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    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="399"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="336"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="518" mass="57646" checksum="7D1FC540450970E5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5D4</accession>
    <name>A0A673B5D4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B5D4"/>
    <dbReference type="Ensembl" id="ENSSORT00005036643.1">
        <property type="protein sequence ID" value="ENSSORP00005035688.1"/>
        <property type="gene ID" value="ENSSORG00005016853.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5D4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="40" mass="4744" checksum="C6240E0E17B11145" modified="2020-06-17" version="1">MASYDKIKYLEYLSLVSKVCTDLENHQGISEKDFGNYFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B8F9</accession>
    <name>A0A673B8F9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B8F9"/>
    <dbReference type="Ensembl" id="ENSSORT00005039625.1">
        <property type="protein sequence ID" value="ENSSORP00005038631.1"/>
        <property type="gene ID" value="ENSSORG00005018049.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B8F9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050795">
        <property type="term" value="P:regulation of behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046883">
        <property type="term" value="P:regulation of hormone secretion"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000248">
        <property type="entry name" value="5-hydroxytryptamine receptor 1A-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000610">
        <property type="entry name" value="5HT1A_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF191">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00512">
        <property type="entry name" value="5HT1ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0085">Behavior</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="19"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="386" mass="43399" checksum="5AE834487040C7D3" modified="2020-06-17" version="1">YQVLTSFLLGALILCAIFGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNRWTLGQVPCDIFVSLDVLCCTSSILHLCAIALDRYWAITEPIDYMKKRTPRRAAVLISVTWLVGFSISVPPMLIMRSQPRSVAEDRANPTQCQISTDPWYTIYSTFGAFYIPLTLMLVLYGRIFKAARFRIRRTVRKTEKKKVSDSCLALSPALFHKKPTAEAHGKSWRRTAEPRVLPLSVNGAVKHAEDGESLEIIEVHGGSRGNLPLPDTPGSAPLFQNRHEKATEAKRKIALARERKTVKTLGIIMGTFILCWLPFFIVALVKPFCPDSRFMPRWLEDVINWLGYSNSLLNPIIYAYFNKDFQSAFNKIIKCHFCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9N8</accession>
    <name>A0A673B9N8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">LIM homeobox 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">lhx4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B9N8"/>
    <dbReference type="FunCoup" id="A0A673B9N8">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039009.1">
        <property type="protein sequence ID" value="ENSSORP00005038019.1"/>
        <property type="gene ID" value="ENSSORG00005017827.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B9N8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09468">
        <property type="entry name" value="LIM1_Lhx4"/>
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    <dbReference type="CDD" id="cd09376">
        <property type="entry name" value="LIM2_Lhx3_Lhx4"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000120">
        <property type="entry name" value="Insulin gene enhancer protein ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000219">
        <property type="entry name" value="LIM/homeobox protein Lhx3"/>
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    </dbReference>
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        <property type="entry name" value="LIM/homeobox protein Lhx3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049594">
        <property type="entry name" value="Lhx3/4-like_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047956">
        <property type="entry name" value="LHX4_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF116">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN LHX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="68"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="136"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="138"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="334"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="369" mass="40537" checksum="952517848050525E" modified="2020-06-17" version="1">SFIFKIPQCAGCSQHILDKFILKVLDRHWHSKCLKCADCQTPLADKCFSRAGSVYCKEDFFKLVRFGTKCASCQQGIPPTQVVRKAQDFVYHLHCFACIMCSRQLATGDEFYLMEDGRLVCKVDYETAKQNDDSEAGTKRPRTTITAKQLETLKSAYKNSPKPARHVREQLSSETGLDMRVVQVWFQNRRAKEKRLKKDAGRHRWSQFYKSVKRSRGGAKVEKESSADDAGLSDSELSFRDDQVLSDLSHTNGLYGSVGDVTNNGILNGGFSVDAAGQPYHDIRAGSPYGLPQSPSSIASLPGHTPLLNNLGFNMDSLVAQGGPGGVGQALRAMAGGPSSDLSTGSSTGYPDFPTSPASWLDEMDHSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BBX9</accession>
    <name>A0A673BBX9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 71</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tmem71</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BBX9"/>
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        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040987.1">
        <property type="protein sequence ID" value="ENSSORP00005039951.1"/>
        <property type="gene ID" value="ENSSORG00005018645.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BBX9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027975">
        <property type="entry name" value="TMEM71"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35255">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35255:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15121">
        <property type="entry name" value="TMEM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="41721" checksum="40F8ADC73E261581" modified="2020-06-17" version="1">MSLFFSGAVTSSPIKRRLRSSLGCQSLDVSLLSPDSSYVCYSSLDGTDPCSCRRSPRLLTNGYYDVTDDSFAWDDDGNVSLTPCKTSVSYKENLVRVFRRRRRPRSSLVRLLSGVTETCQSWLDENVFRGVFRAAQNQDQEVDESQDRDQDHRPDWTRTSLDESFWSGISSTEVDDGRSFTYDPTEVPPPPNKEAPPPKMLIQEEICSEICQSQERFTQSLPGLSEVPPPSVFYTNSCCCQASPEPTGFTMKTLLLFIFSVFIFTALYSGCLWRGVAVATTVFVIISTFMLMSKSGPMGEWRRAKTEVSLKTNQSHVTSSTRNTTLTLCLMTAFSRTSHPGTSRRGGGRGAKTKTNGMLGIEWMLRAFWRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BDG0</accession>
    <name>A0A673BDG0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ubl3a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BDG0"/>
    <dbReference type="FunCoup" id="A0A673BDG0">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039430.1">
        <property type="protein sequence ID" value="ENSSORP00005038438.1"/>
        <property type="gene ID" value="ENSSORG00005017959.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BDG0"/>
    <dbReference type="OrthoDB" id="1043111at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17048">
        <property type="entry name" value="Ubl_UBL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000167">
        <property type="entry name" value="Ubiquitin-like 3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017000">
        <property type="entry name" value="MUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040015">
        <property type="entry name" value="UBL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047977">
        <property type="entry name" value="UBL3_Ubl_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032572">
        <property type="entry name" value="MUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR032572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="117" mass="13066" checksum="DB22A3027AFC3576" modified="2020-06-17" version="1">MTGSTPADMINLRLILVSGKTKEFLFSPNDSAADIAKHVYDNWPMDWEEEQVSSPNILRLIYQGRFLHGNVTLGALKLPLGKTTVMHLVARETLPEPNSQGQRNREKTGESNCCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJ45</accession>
    <name>A0A673BJ45_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear receptor subfamily 1 group D member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">nr1d4b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BJ45"/>
    <dbReference type="Ensembl" id="ENSSORT00005042336.1">
        <property type="protein sequence ID" value="ENSSORP00005041274.1"/>
        <property type="gene ID" value="ENSSORG00005019219.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJ45"/>
    <dbReference type="OrthoDB" id="7634782at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07166">
        <property type="entry name" value="NR_DBD_REV_ERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000013">
        <property type="entry name" value="Nuclear receptor subfamily 1 group D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000039">
        <property type="entry name" value="Nuclear receptor subfamily 1, group D, member 4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001728">
        <property type="entry name" value="ThyrH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF489">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 1 GROUP D MEMBER 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00546">
        <property type="entry name" value="THYROIDHORMR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="116"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="344"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="216"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="263"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="233"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="579" mass="63190" checksum="E2C9872EBB9E173B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BL23</accession>
    <name>A0A673BL23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Phosphatidylinositol 3-kinase catalytic subunit type 3</fullName>
            <ecNumber evidence="4 16">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pik3c3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="4 16"/>
    <dbReference type="RefSeq" id="XP_030017920.1">
        <property type="nucleotide sequence ID" value="XM_030162060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BL23"/>
    <dbReference type="Ensembl" id="ENSSORT00005043554.1">
        <property type="protein sequence ID" value="ENSSORP00005042475.1"/>
        <property type="gene ID" value="ENSSORG00005019093.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438430"/>
    <dbReference type="CTD" id="5289"/>
    <dbReference type="InParanoid" id="A0A673BL23"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08397">
        <property type="entry name" value="C2_PI3K_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00870">
        <property type="entry name" value="PI3Ka_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000043">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="19">
        <location>
            <begin position="34"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="18">
        <location>
            <begin position="282"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="17">
        <location>
            <begin position="593"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="875" mass="99740" checksum="A5F42CD03D73DED0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNR0</accession>
    <name>A0A673BNR0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cysteine sulfinic acid decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">csad</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BNR0"/>
    <dbReference type="FunCoup" id="A0A673BNR0">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044494.1">
        <property type="protein sequence ID" value="ENSSORP00005043400.1"/>
        <property type="gene ID" value="ENSSORG00005020042.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BNR0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004782">
        <property type="term" value="F:sulfinoalanine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042412">
        <property type="term" value="P:taurine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF8">
        <property type="entry name" value="CYSTEINE SULFINIC ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="486" mass="54943" checksum="EFABA75EE278C71C" modified="2025-10-08" version="2">MGNVGQTDLHDLNEPIINHSEGQLFLSEAFKIIVEEVLCKGTDVNEKVCQWKEPEELAALLDLELRETGEPQHQLLQRIKDVAKYSIKTSHPRFFNQQFGGVDYHSLAGRFLSETLNTNLFTYEVAPVFILMESEVLRGLRLLVGWTHGDGLFCPGGSTSNMYAMNLARYQLFPEVKSQGLWGLPRLAIFTSPESHYSVKKGAAFLGIGTDNVILVKVDDGGRMIPEDLDEKIKQAKSQGAVPFLVSCTSGTTVQGAFDPLDHIADVCEKHKLWMHVDAAWGGSVLFSKQHRHLMKGVDRANSVAWNPHKMLVAGLQCSVLLLRDTTNLLKQCHSANATYLFQQDKFYDVNLDVGDKSVQCSRKVDCLKLWLMWKAVGSSGLAERVDKAFVNVRYLTEKMKKREGFQILREPEFVNLCFWYIPPMNCLDHTPVIKERMMKQGSMMVGYQPLGNKVNFFRVVVFSPLLSTKDLDFFLNEIERLGNDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRF5</accession>
    <name>A0A673BRF5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BRF5"/>
    <dbReference type="Ensembl" id="ENSSORT00005046531.1">
        <property type="protein sequence ID" value="ENSSORP00005045381.1"/>
        <property type="gene ID" value="ENSSORG00005020847.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRF5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025612944" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="30"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="32"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="127" mass="14523" checksum="CB5053C41A967A89" modified="2020-06-17" version="1">IITALKFVKIISLSNVLHLLLLGSISVNSIRPQEVEVSETEGKSVTLTCDTENSDSDINLHWYRQHSDLQPPHFILWKRAQNSPTQYIRDLYRGRFTSETPDQTTQLTIKDLTLADTALYYCALETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUA5</accession>
    <name>A0A673BUA5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">trappc4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Core component of the TRAPP complexes which has a function of guanine nucleotide exchange factor activity for Rab1 GTPase. Plays a role in vesicular transport from endoplasmic reticulum to Golgi and autophagy. May play a role in dendrite postsynaptic membrane trafficking.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Interacts with SDC2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus</location>
            <location evidence="15">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030008783.1">
        <property type="nucleotide sequence ID" value="XM_030152923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BUA5"/>
    <dbReference type="Ensembl" id="ENSSORT00005046530.1">
        <property type="protein sequence ID" value="ENSSORP00005045380.1"/>
        <property type="gene ID" value="ENSSORG00005020837.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432100"/>
    <dbReference type="CTD" id="51399"/>
    <dbReference type="InParanoid" id="A0A673BUA5"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000002">
        <property type="entry name" value="Trafficking protein particle complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 15" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="219" mass="24358" checksum="6E346019A1397FEF" modified="2020-06-17" version="1">MVIFSVYVVNKAGGLIYQYDNYVPRAEAEKTFSYPLDLVLKHHDEKVIVSFGQRDGIRVGHAVLSINGVDVNGKNTADGKDILEYFKDPANYPVSIRFGRARLSSNEKLMLASMFHSLFAIGSQLSPEVGSSGIEMLETDVFKLHCFQTLTGIKFIVLADPRQSGIDALLRKIYEVYSDFALKNPFYSLEMPIRCELFDQNLKSALEVAEKAGNFGAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUN5</accession>
    <name>A0A673BUN5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ninein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ninl</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BUN5"/>
    <dbReference type="Ensembl" id="ENSSORT00005045972.1">
        <property type="protein sequence ID" value="ENSSORP00005044839.1"/>
        <property type="gene ID" value="ENSSORG00005020617.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUN5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18905">
        <property type="entry name" value="NINEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18905:SF12">
        <property type="entry name" value="NINEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="211"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="106"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="554"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="702"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="802"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1143"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="924"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1061"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="804"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1264" mass="145317" checksum="6EE95AF4452BA6FB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUZ8</accession>
    <name>A0A673BUZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mpped1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0046 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004129.1">
        <property type="nucleotide sequence ID" value="XM_030148269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BUZ8"/>
    <dbReference type="FunCoup" id="A0A673BUZ8">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045111.1">
        <property type="protein sequence ID" value="ENSSORP00005043993.1"/>
        <property type="gene ID" value="ENSSORG00005020291.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429033"/>
    <dbReference type="CTD" id="758"/>
    <dbReference type="InParanoid" id="A0A673BUZ8"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07379">
        <property type="entry name" value="MPP_239FB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024201">
        <property type="entry name" value="Calcineurin-like_Pesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051693">
        <property type="entry name" value="UPF0046_metallophosphoest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905:SF31">
        <property type="entry name" value="METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035808">
        <property type="entry name" value="Pdiesterase_Brain_239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="3">
        <location>
            <begin position="77"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="312" mass="35748" checksum="A36D3D01446EF702" modified="2020-06-17" version="1">MAFTLSEIMAARRQQQSQAQSHTQRGSRSAVEVDEYSTNPTQAFTFYNINQGRFQPPHVHMVDPMPHDTPKPPGYTRFVCISDTHSRTDAIQMPYGDVFIHAGDFTELGLPSEVKKFNDWLGTLPYDIKIVIAGNHELTFDQEFMADLIKQDFYYFPSASKLKPENYENVQSLLTNCIYLQDSEVTVRGFRIYGSPWQPWYYGWGFNLPRGQALLDKWNQIPDNTDILVTHCPPLGFLDWVPKKMQRVGCMELLNTVQRRVQPKLHVFGHIHEGYGMMTDGTTTFVNASACTVNFLPMNPPIVFDLPNPRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVE1</accession>
    <name>A0A673BVE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ugt5f1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673BVE1"/>
    <dbReference type="FunCoup" id="A0A673BVE1">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045650.1">
        <property type="protein sequence ID" value="ENSSORP00005044527.1"/>
        <property type="gene ID" value="ENSSORG00005020499.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVE1"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF48">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY, POLYPEPTIDE C2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025706013" description="UDP-glucuronosyltransferase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="527" mass="60162" checksum="8FDEA34B97D8B4F5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYI6</accession>
    <name>A0A673BYI6_9TELE</name>
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        <submittedName>
            <fullName evidence="10">Ras association domain-containing protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rassf5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BYI6"/>
    <dbReference type="FunCoup" id="A0A673BYI6">
        <property type="interactions" value="407"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047256.1">
        <property type="protein sequence ID" value="ENSSORP00005046099.1"/>
        <property type="gene ID" value="ENSSORG00005021119.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BYI6"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20886">
        <property type="entry name" value="C1_RASSF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21892">
        <property type="entry name" value="SARAH_RASSF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033614">
        <property type="entry name" value="RASSF1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011524">
        <property type="entry name" value="SARAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22738:SF9">
        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22738">
        <property type="entry name" value="RASSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16517">
        <property type="entry name" value="Nore1-SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50951">
        <property type="entry name" value="SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="7">
        <location>
            <begin position="227"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="8">
        <location>
            <begin position="387"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="SARAH" evidence="9">
        <location>
            <begin position="483"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="39"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="297"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="532" mass="59101" checksum="103F636A436409DD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYL9</accession>
    <name>A0A673BYL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">myca</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes.</text>
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    <comment type="subunit">
        <text evidence="8">Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049264.1">
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        <property type="gene ID" value="ENSSORG00005021951.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BYL9"/>
    <dbReference type="OrthoDB" id="5964374at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11458">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000019">
        <property type="entry name" value="Myc proto-oncogene protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003327">
        <property type="entry name" value="Myc-LZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050433">
        <property type="entry name" value="Myc_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002418">
        <property type="entry name" value="Tscrpt_reg_Myc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012682">
        <property type="entry name" value="Tscrpt_reg_Myc_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45851">
        <property type="entry name" value="MYC PROTO-ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02344">
        <property type="entry name" value="Myc-LZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01056">
        <property type="entry name" value="Myc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001705">
        <property type="entry name" value="Myc_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00044">
        <property type="entry name" value="LEUZIPPRMYC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="339"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="191"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="300"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="391"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="424" mass="47302" checksum="07D51A81054E623A" modified="2020-06-17" version="1">MPLNSSLASKNYDYDYDSLQPYFYDNEEEAFYPQQLQPPAPSEDIWKKFELLPTPPLSPSRRPSLSSLFPSTADQLEMVTEFLGDDVVNQSIICDADYSQSFLKSIIIQDCMWSGFSAAAKLEKVVSERLASLQAARKECPAGDSADPGVGVGVGAAQWRLNSSYLQDLNTAASECIDPSVVFPYPIAEMPKQNTVTPPSKDLGLDTPPNSGSSSSSCSDSEDEDGDDDEEEEDQEEEEEIDVVTVEKRQAVKRCDPSPVETRHPSPLVLKRCHVSTHQHNYAAHPSMRHEQPAVKRLKLESGSGSVSGASSGHSRVLKQISSNRKCSSPRTSDTEDYDKRRTHNVLERQRRNELKLSFFALRDEIPEVANNEKAAKVVILKKATECIYSMQSDEQRLLSLKEQLRRKSELLKQRLAQLQGSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZV0</accession>
    <name>A0A673BZV0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytoplasmic FMR1-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cyfip1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CYFIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BZV0"/>
    <dbReference type="FunCoup" id="A0A673BZV0">
        <property type="interactions" value="1330"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046818.1">
        <property type="protein sequence ID" value="ENSSORP00005045663.1"/>
        <property type="gene ID" value="ENSSORG00005020938.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BZV0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008081">
        <property type="entry name" value="Cytoplasmic_FMR1-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12195">
        <property type="entry name" value="CYTOPLASMIC FMR1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05994">
        <property type="entry name" value="FragX_IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008153">
        <property type="entry name" value="FMR1_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01698">
        <property type="entry name" value="CYTOFMRPINTP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="4">
        <location>
            <begin position="64"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR008153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1258" mass="145881" checksum="DADF495DA41A7D6F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C350</accession>
    <name>A0A673C350_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C350"/>
    <dbReference type="Ensembl" id="ENSSORT00005050964.1">
        <property type="protein sequence ID" value="ENSSORP00005049761.1"/>
        <property type="gene ID" value="ENSSORG00005022571.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C350"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="123"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="288" mass="32659" checksum="4E1BBE0C13261FF8" modified="2020-06-17" version="1">MEEDYRSASRKFWQTIRRLRRGKQCATNTVYSGSGELLTSTGDVVGRWKEYFEDLLNPTATSSIEEVEAEVSEVDSSITQAEVTEVVGKLLGGRAPGVDEIRPEYLKSLDVQGLSWLTRLCNIAWRSGTVPLDWQTGVVVPLFKKGDRRMCSNYRGITLLSLPGKVYSRVLERRIRPIVEPRIQEEQCGFRPGRGTLDQLYTLHRVLEGSWEFAQPVHMCFVDLEKAFDRVPRGILWGVLREYGVRGPLLRAVRSLYDRSRSLVRIAGSKSDLFQVHVGLRQGCPLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4X8</accession>
    <name>A0A673C4X8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115436218</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C4X8"/>
    <dbReference type="Ensembl" id="ENSSORT00005049494.1">
        <property type="protein sequence ID" value="ENSSORP00005048304.1"/>
        <property type="gene ID" value="ENSSORG00005022046.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C4X8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF358">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="66"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="77"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="378" mass="43069" checksum="2147E0EC691D9D99" modified="2020-06-17" version="1">MPRNSSASVFGGAGGGGTRASVSSLQGLRNVMRNEPETDSAPAPTAAPNTASSPPNRAASAAPADDKQTLRGLNDRLGGFLGKVKQLESENRDLEAQIDDILARRKTPDGRNWDDIQKPLDELKEKIKDITKDNANLLLQIDNTNLANNDLKNKLEDEKKARKAIEKDLEDQRKTIDETKLKCTHLKKDIELVKNELESLKEDHKDVKDLREKIKDSEVRVEIKSEESNLADVLNKIRVQYDELAKKNLKDTDDWYQRKFENIKVVEAQNTETLKSGKEDLKELLKEKQLLQIKIQSALSTIHTLEESVSATKVEYSHRLTPLYKMIRDLEAELKEMRSLEEHQHEVNTNLLYVKMKLEEEINNYQQLIHGMTTDPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C8D7</accession>
    <name>A0A673C8D7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Eukaryotic translation initiation factor 2-alpha kinase 3</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">PRKR-like endoplasmic reticulum kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Protein tyrosine kinase EIF2AK3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">eif2ak3</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:10597"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Forms dimers with HSPA5/BIP in resting cells. Homotetramerizes in response to endoplasmic reticulum (ER) stress, leading to its activation. Interacts with HSP90B1/GRP94. Interacts with DNAJC3; inhibiting EIF2AK3/PERK activity. Interacts with ATAD3A; ATAD3A and EIF2S1/eIF-2-alpha occupy a common binding site within the cytoplasmic loop of EIF2AK3/PERK, leading to prevent EIF2AK3/PERK association with its substrate EIF2S1/eIF-2-alpha. Interacts with MFN2. Interacts with TMEM33. Interacts with PDIA6. Interacts with LACC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_029982468.1">
        <property type="nucleotide sequence ID" value="XM_030126608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C8D7"/>
    <dbReference type="FunCoup" id="A0A673C8D7">
        <property type="interactions" value="1225"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049635.1">
        <property type="protein sequence ID" value="ENSSORP00005048443.1"/>
        <property type="gene ID" value="ENSSORG00005021945.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413617"/>
    <dbReference type="CTD" id="9451"/>
    <dbReference type="InParanoid" id="A0A673C8D7"/>
    <dbReference type="OrthoDB" id="341578at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09768">
        <property type="entry name" value="Luminal_EIF2AK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14048">
        <property type="entry name" value="STKc_EIF2AK3_PERK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000251">
        <property type="entry name" value="eukaryotic translation initiation factor 2-alpha kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000622">
        <property type="entry name" value="Eukaryotic translation initiation factor 2-alpha kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000193">
        <property type="entry name" value="Eukaryotic translation initiation factor 2-alpha kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF166">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="12 28" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 30" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025372967" description="Eukaryotic translation initiation factor 2-alpha kinase 3" evidence="30">
        <location>
            <begin position="32"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="599"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="758"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="803"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="29">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="817"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="628"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00062422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00069562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00078389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1101" mass="122713" checksum="82D1DD2F51472EF2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C8M0</accession>
    <name>A0A673C8M0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="8">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gldc</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GcvP family.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="8"/>
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        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:lyase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:pyridoxal phosphate binding"/>
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        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
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        <property type="entry name" value="Glycine cleavage system P protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glycine dehydrogenase (decarboxylating)"/>
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        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
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        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
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        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
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        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
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        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
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        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
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        <property type="entry name" value="Beta_elim_lyase"/>
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        <property type="entry name" value="GcvP2_C"/>
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        <property type="entry name" value="PLP-dependent transferases"/>
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    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
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        <location>
            <begin position="76"/>
            <end position="495"/>
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    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="9">
        <location>
            <begin position="593"/>
            <end position="736"/>
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        <location>
            <begin position="836"/>
            <end position="953"/>
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    </feature>
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        <location>
            <position position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1012" mass="112597" checksum="5E0977F9E697749D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9N9</accession>
    <name>A0A673C9N9_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="14" type="primary">ptpn21</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005049984.1"/>
        <property type="gene ID" value="ENSSORG00005022683.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13188">
        <property type="entry name" value="FERM_C_PTPN14_PTPN21"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000149">
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000030">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
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    </dbReference>
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        <property type="entry name" value="Band_41_domain"/>
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    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
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    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
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    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
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    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014392">
        <property type="entry name" value="PTP_non-rcpt_14/21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041782">
        <property type="entry name" value="PTPN14/21_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF3">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
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    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000934">
        <property type="entry name" value="Tyr-Ptase_nr14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="13">
        <location>
            <begin position="23"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="932"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="12">
        <location>
            <begin position="1113"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="397"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="761"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="833"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="397"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="765"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="845"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="902"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="9">
        <location>
            <position position="1143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000934-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1209" mass="137871" checksum="E4F9B978B1EA0A4E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAR4</accession>
    <name>A0A673CAR4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">bnip3lb</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CAR4"/>
    <dbReference type="Ensembl" id="ENSSORT00005051050.1">
        <property type="protein sequence ID" value="ENSSORP00005049847.1"/>
        <property type="gene ID" value="ENSSORG00005022617.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CAR4"/>
    <dbReference type="OrthoDB" id="5857140at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097345">
        <property type="term" value="P:mitochondrial outer membrane permeabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010548">
        <property type="entry name" value="BNIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186:SF10">
        <property type="entry name" value="BCL2 INTERACTING PROTEIN 3 LIKE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186">
        <property type="entry name" value="RE48077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06553">
        <property type="entry name" value="BNIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="221" mass="24018" checksum="180D8D68639673E3" modified="2020-06-17" version="1">MSDAANATSTDNNGDSGLNGSWVELEMNRGTAASVQSTSTAGLASGLLPLPQVEEEEAMVGGLEHVPSSSSIHNGDMEKILLDAQHESSRSNSSCDSPPRPHSPQDEGQIIFDVDGRRDSQSEEDALDKERDMDILMKDADWVADWSSRPENIPPKEFHFRHPRRSVTLSMRKTGVMKKGGIFSAEFLKVFIPSLLLSHILALGLGVYIGKRLTTPPASSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBZ5</accession>
    <name>A0A673CBZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphoglucomutase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pgm2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBZ5"/>
    <dbReference type="FunCoup" id="A0A673CBZ5">
        <property type="interactions" value="1000"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052030.1">
        <property type="protein sequence ID" value="ENSSORP00005050814.1"/>
        <property type="gene ID" value="ENSSORG00005022532.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBZ5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000017">
        <property type="entry name" value="glucose 1,6-bisphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF3">
        <property type="entry name" value="PHOSPHOPENTOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="55"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="228"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="344"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="67914" checksum="4409871160F1C22C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CC01</accession>
    <name>A0A673CC01_9TELE</name>
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        <submittedName>
            <fullName evidence="6">La-related protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">larp4aa</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CC01"/>
    <dbReference type="FunCoup" id="A0A673CC01">
        <property type="interactions" value="356"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052838.1">
        <property type="protein sequence ID" value="ENSSORP00005051605.1"/>
        <property type="gene ID" value="ENSSORG00005023306.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CC01"/>
    <dbReference type="OrthoDB" id="10046764at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08035">
        <property type="entry name" value="LARP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12707">
        <property type="entry name" value="RRM_LARP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034903">
        <property type="entry name" value="LARP4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058699">
        <property type="entry name" value="RRM_LARP4/4B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF48">
        <property type="entry name" value="LA-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26088">
        <property type="entry name" value="RRM_LARP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="5">
        <location>
            <begin position="139"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="436"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="563"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="458"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="485"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="636"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="712"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="730"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="805" mass="87317" checksum="3929B7C86418515F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCH7</accession>
    <name>A0A673CCH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cadherin-12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115437735</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCH7"/>
    <dbReference type="Ensembl" id="ENSSORT00005054541.1">
        <property type="protein sequence ID" value="ENSSORP00005053286.1"/>
        <property type="gene ID" value="ENSSORG00005023957.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCH7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000014">
        <property type="entry name" value="Cadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000097">
        <property type="entry name" value="cadherin-12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF96">
        <property type="entry name" value="CADHERIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="625"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="102"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="183"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="292"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="405"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="509"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="698"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="701"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="810" mass="88661" checksum="43FC5BEF1F50A608" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CCR0</accession>
    <name>A0A673CCR0_9TELE</name>
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        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CCR0"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051734.1">
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        <property type="gene ID" value="ENSSORG00005022894.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CCR0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF112">
        <property type="entry name" value="VENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="75"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="77"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="190" mass="22443" checksum="855CFFF98FFF00B7" modified="2020-06-17" version="1">ITMANFTVEWLCQSYYRYTEETHLHDEEEEEVHKNTEEFTEEPKHQYSPTSPNNSCGYSSSSDSEAGDESDGEAAQNPRMRTKFTSEQIRKLENTFSEHKYLGSTQRRRIAHKLNLSETQVNTWFQNRRMKMKREIQDMRTEFLTVPTALLPPLLFQHHGLSGQLPVIHIFCKRLVYKCKHFCVILLFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CEA3</accession>
    <name>A0A673CEA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tudor domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDRD5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CEA3"/>
    <dbReference type="FunCoup" id="A0A673CEA3">
        <property type="interactions" value="770"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052872.1">
        <property type="protein sequence ID" value="ENSSORP00005051639.1"/>
        <property type="gene ID" value="ENSSORG00005023313.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CEA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
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    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
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    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF19">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="11">
        <location>
            <begin position="60"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="11">
        <location>
            <begin position="181"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="250"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="658" mass="74195" checksum="492FD56A4548AD97" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFG7</accession>
    <name>A0A673CFG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Syndecan</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">sdc3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Membrane</location>
            <topology evidence="1 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neurexin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the syndecan proteoglycan family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030003165.1">
        <property type="nucleotide sequence ID" value="XM_030147305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CFG7"/>
    <dbReference type="Ensembl" id="ENSSORT00005054128.1">
        <property type="protein sequence ID" value="ENSSORP00005052875.1"/>
        <property type="gene ID" value="ENSSORG00005023827.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428320"/>
    <dbReference type="CTD" id="9672"/>
    <dbReference type="InParanoid" id="A0A673CFG7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001050">
        <property type="entry name" value="Syndecan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027789">
        <property type="entry name" value="Syndecan/Neurexin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030479">
        <property type="entry name" value="Syndecan_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10915">
        <property type="entry name" value="SYNDECAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10915:SF7">
        <property type="entry name" value="SYNDECAN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01034">
        <property type="entry name" value="Syndecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00964">
        <property type="entry name" value="SYNDECAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025473580" description="Syndecan" evidence="13">
        <location>
            <begin position="21"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Neurexin/syndecan/glycophorin C" evidence="14">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="71"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="308"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="111"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="452" mass="50563" checksum="C86F4BD7FC798EDC" modified="2020-06-17" version="1">MKLPWVVLALTALVVHAATGQTWSDDEGSTRDDFFDDEDLYSGSGSGFPEISMRPTSVGVSFTTEETLLLSTTQATSPAPSASPAAEPSPNPDPTTIPLTTEADGESGAFWERERELEREREREMEMEKERERQREMERERERERVREMEREREREREREREREREREREREREREKERERQRERERERERELERIRAAQTTPAPRSPAAVPVATTNPATSTAESAAPSAGSDDLSTTEEEEDDVYLSPEPTSSYPGFDMETTTEEDTSTTAETTSEATTAPPTTTRTTTAATTTVKTRVPFRPRVPMTTATQAVPTEKTTRTPQPTTTQGRSEEGNNEVGAAGPSGDFEIREENRNGLGRGLMPGDPDLIGNTVDGGSSAAQLPQKNILERKEVLIAVIVGGVVGALFAAFLVMLLVYRMKKKDEGSYTLEEPKQATVTYQKPDKQEEFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CH45</accession>
    <name>A0A673CH45_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CH45"/>
    <dbReference type="Ensembl" id="ENSSORT00005053267.1">
        <property type="protein sequence ID" value="ENSSORP00005052027.1"/>
        <property type="gene ID" value="ENSSORG00005023478.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CH45"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="53" mass="6217" checksum="F2822FCB03087170" modified="2020-06-17" version="1">MKPQSWIRKNWLWAAGGAFVTIHLTTWLMQRAMKTAVRSETALKQKTTEERLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJ39</accession>
    <name>A0A673CJ39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Grh/CP2 DB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tfcp2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the grh/CP2 family. CP2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJ39"/>
    <dbReference type="FunCoup" id="A0A673CJ39">
        <property type="interactions" value="1104"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056896.1">
        <property type="protein sequence ID" value="ENSSORP00005055611.1"/>
        <property type="gene ID" value="ENSSORG00005024686.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJ39"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09589">
        <property type="entry name" value="SAM_TFCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000022">
        <property type="entry name" value="Transcription factor CP2 like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037599">
        <property type="entry name" value="TFCP2_SAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF11">
        <property type="entry name" value="ALPHA-GLOBIN TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25416">
        <property type="entry name" value="GRHL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="9">
        <location>
            <begin position="62"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="503" mass="57085" checksum="A00097C781BCF494" modified="2020-06-17" version="1">MAWALKLPLTDEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESNLPPDSDNKILPFQYVLCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKIGELPEITGKMVKSIIRVVFHDRRLQYTEHQQLEGWRWNRPGDRILDLDIPMSVGIIDPRANPTQLNTVEFLWDPSKRTSVFIQVHCISTEFTMRKHGGEKGVPFRIQIDTFKENENGEYTEHLHSASCQVKVFKPKGADRKQKTDREKMEKRAPQEKEKYQPSYETTILTECSPWPEVTYVNNSPSPGFNSTHNSFPVAEGNGSPNHQPEPVVQAADSVLFSAQNLLPTATPQDAQQWLHRNRFSPFCRLFTNFSGADLLKLTREDVIQICGPADGIRLFNALKGRVVRPRLTIYVCQESQQAREQQTKHENGDAAANTFFVYHAIYLEELTSAELTEKIAQLFNISPRQINQIFKQGPTGIHVLVSDEMIQNFQDEVCFVLDTMKDDTNDGYHIILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKY3</accession>
    <name>A0A673CKY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">DEAH box protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC115410706</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029978328.1">
        <property type="nucleotide sequence ID" value="XM_030122468.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029978329.1">
        <property type="nucleotide sequence ID" value="XM_030122469.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029978330.1">
        <property type="nucleotide sequence ID" value="XM_030122470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CKY3"/>
    <dbReference type="Ensembl" id="ENSSORT00005057567.1">
        <property type="protein sequence ID" value="ENSSORP00005056271.1"/>
        <property type="gene ID" value="ENSSORG00005025047.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410706"/>
    <dbReference type="InParanoid" id="A0A673CKY3"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000010">
        <property type="entry name" value="ATP-dependent RNA helicase DQX1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001007">
        <property type="entry name" value="putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001055">
        <property type="entry name" value="putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF88">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="66"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="707"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="718"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="738" mass="82905" checksum="1EF69C73D6839AEB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CMB3</accession>
    <name>A0A673CMB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thromboxane A2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Prostanoid TP receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">tbxa2r</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987225.1">
        <property type="nucleotide sequence ID" value="XM_030131365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CMB3"/>
    <dbReference type="Ensembl" id="ENSSORT00005055723.1">
        <property type="protein sequence ID" value="ENSSORP00005054449.1"/>
        <property type="gene ID" value="ENSSORG00005024390.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417442"/>
    <dbReference type="CTD" id="6915"/>
    <dbReference type="InParanoid" id="A0A673CMB3"/>
    <dbReference type="OrthoDB" id="8631411at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004960">
        <property type="term" value="F:thromboxane receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045777">
        <property type="term" value="P:positive regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045907">
        <property type="term" value="P:positive regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000163">
        <property type="entry name" value="Thromboxane A2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001105">
        <property type="entry name" value="Thbox_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF5">
        <property type="entry name" value="THROMBOXANE A2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00429">
        <property type="entry name" value="THROMBOXANER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="199"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="45"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="343"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="373" mass="41856" checksum="880DECD505876679" modified="2020-06-17" version="1">MNASASSSLNGTPLCYSINSPPFNYHPTIASANFSIIFGTLGVASNLIAFIVLIKSFRRTQSRSRSFFLIFLGGLVITDFMGLLVTASIVISFHVTHFNWRELDPHCHFCNFMGMSMVFYGLCPLLLGAAMAVERFIGINRPFARSTSMPKTRTISMVLMVWLIAGCITLLPLMGIGSYHMQMPGSWCFFNISADGTDLAFSLLFSLVGLISITVSFLLNTVSVVTLIKVCCGQDRTQRRRDHEIEMMVQLILIMVIASICWCPLLVFIAQTVLSGSRLQIRYLLLWIRFATWNQILDPWVYILFRRAVLRRIHPRLDWSRGSIMTLYPSFSDTVRRFTRPSLGSALSQDDSGETEKSNATSPSTLKPRSPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRV4</accession>
    <name>A0A673CRV4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="8">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RING-type E3 ubiquitin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mgrn1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673CRV4"/>
    <dbReference type="Ensembl" id="ENSSORT00005058353.1">
        <property type="protein sequence ID" value="ENSSORP00005057050.1"/>
        <property type="gene ID" value="ENSSORG00005025328.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CRV4"/>
    <dbReference type="OrthoDB" id="10014838at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043951">
        <property type="term" value="P:negative regulation of cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045744">
        <property type="term" value="P:negative regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000013">
        <property type="entry name" value="E3 ubiquitin-protein ligase MGRN1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045194">
        <property type="entry name" value="MGRN1/RNF157-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058981">
        <property type="entry name" value="MGRN1/RNF157-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MGRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996">
        <property type="entry name" value="MAHOGUNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26192">
        <property type="entry name" value="RNF157-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="397"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="514"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="527"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="549" mass="60405" checksum="949A3FFA272C9C72" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSF8</accession>
    <name>A0A673CSF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">zranb1a</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Ubiquitin thioesterase, which specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin. Also cleaves 'Lys-63'-linked chains, but with 40-fold less efficiency compared to 'Lys-29'-linked ones. Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription. Acts as a regulator of autophagy by mediating deubiquitination of PIK3C3/VPS34, thereby promoting autophagosome maturation. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C64 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673CSF8"/>
    <dbReference type="FunCoup" id="A0A673CSF8">
        <property type="interactions" value="1023"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057595.1">
        <property type="protein sequence ID" value="ENSSORP00005056299.1"/>
        <property type="gene ID" value="ENSSORG00005025055.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSF8"/>
    <dbReference type="OrthoDB" id="6275030at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071947">
        <property type="term" value="P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035523">
        <property type="term" value="P:protein K29-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990168">
        <property type="term" value="P:protein K33-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22767">
        <property type="entry name" value="OTU_ZRANB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.560:FF:000001">
        <property type="entry name" value="ubiquitin thioesterase ZRANB1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000006">
        <property type="entry name" value="ubiquitin thioesterase ZRANB1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000012">
        <property type="entry name" value="ubiquitin thioesterase ZRANB1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041294">
        <property type="entry name" value="AnkUBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049768">
        <property type="entry name" value="ZRANB1_OTU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF28">
        <property type="entry name" value="UBIQUITIN THIOESTERASE ZRANB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18418">
        <property type="entry name" value="AnkUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="21">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="21">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="21">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="OTU" evidence="22">
        <location>
            <begin position="445"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00059005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="720" mass="82477" checksum="BBFF19AF8EA87A67" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CZA0</accession>
    <name>A0A673CZA0_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">Enhancer of polycomb homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">epc1a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CZA0"/>
    <dbReference type="FunCoup" id="A0A673CZA0">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059645.1">
        <property type="protein sequence ID" value="ENSSORP00005058322.1"/>
        <property type="gene ID" value="ENSSORG00005025751.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="435275at2759"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009607">
        <property type="entry name" value="Enhancer_polycomb_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06752">
        <property type="entry name" value="E_Pc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Enhancer of polycomb C-terminal" evidence="9">
        <location>
            <begin position="597"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="313"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="470"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="809"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="493"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="819"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="844" mass="93119" checksum="D403F1DDE8D79413" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673D025</accession>
    <name>A0A673D025_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DMAP1-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115411281</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DIP2 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005059269.1">
        <property type="protein sequence ID" value="ENSSORP00005057948.1"/>
        <property type="gene ID" value="ENSSORG00005023885.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673D025"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="CDD" id="cd05905">
        <property type="entry name" value="Dip2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000003">
        <property type="entry name" value="DIP2 disco-interacting protein 2 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000001">
        <property type="entry name" value="DIP2 disco-interacting protein 2 homolog C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037337">
        <property type="entry name" value="Dip2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF33">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 HOMOLOG C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23024">
        <property type="entry name" value="AMP-dom_DIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01137">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51912">
        <property type="entry name" value="DMAP1_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="DMAP1-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1597" mass="174716" checksum="A521D820C2FC956C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y264</accession>
    <name>A0A672Y264_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ivns1abpa</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y264"/>
    <dbReference type="FunCoup" id="A0A672Y264">
        <property type="interactions" value="658"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000107.1">
        <property type="protein sequence ID" value="ENSSORP00005000092.1"/>
        <property type="gene ID" value="ENSSORG00005000059.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y264"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd18502">
        <property type="entry name" value="BACK_NS1BP_IVNS1ABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18306">
        <property type="entry name" value="BTB_POZ_NS1BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF396">
        <property type="entry name" value="INFLUENZA VIRUS NS1A-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="50"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="657" mass="73341" checksum="06F8A9F86CC4BE62" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3G4</accession>
    <name>A0A672Y3G4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heat shock transcription factor 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y3G4"/>
    <dbReference type="Ensembl" id="ENSSORT00005000320.1">
        <property type="protein sequence ID" value="ENSSORP00005000298.1"/>
        <property type="gene ID" value="ENSSORG00005000219.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y3G4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF213">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="9">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="251" mass="28778" checksum="AD3B2B1E2659ABE7" modified="2020-06-17" version="1">SVGGGGGGGGAVYTSNVPAFLTKLWTLVEDPDTNHLICWSAVNWNQFPRHSSGTLCKEVLPKYFKHNNMASFVRQLNMYGFRKVVNIEQSGLVKPERDDTEFQHLYFLQGHEHMLEHIKRKVSIVKSEETKVRQEDLSKLLYEVQLLRTQQDNMEMQNEVLWREVVSLRQNHTQQQKVMNRSLIQFLFSQMQSNTPSTVGLKRKLRAINKSPTNRAEGRLAQLTNTTSNATSCEWTQVLNQTHTHNTHNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4W8</accession>
    <name>A0A672Y4W8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GTPase IMAP family member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Immune-associated nucleotide-binding protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">si:dkey-110g7.8</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Exerts an anti-apoptotic effect in the immune system and is involved in responses to infections.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y4W8"/>
    <dbReference type="Ensembl" id="ENSSORT00005001167.1">
        <property type="protein sequence ID" value="ENSSORP00005001133.1"/>
        <property type="gene ID" value="ENSSORG00005000678.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4W8"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000536">
        <property type="entry name" value="GTPase IMAP family member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF139">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
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        <property type="entry name" value="G_AIG1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="18"/>
            <end position="241"/>
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            <end position="471"/>
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            <end position="493"/>
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    </feature>
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        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004240"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="493" mass="55249" checksum="5A22DF9DDFD08577" modified="2020-06-17" version="1">MAAVSSASDTGDPRGRHPPERRLLLLGGPQSGKTCTANSILGDEVFDTGTETTHSNVGQTEIYGRRVTVVDTPPWAIPNDPEEDTETDHNDNAGAESDSPRRPPPSLDSEGPCMGAILCPPGPHAILLVVSVTQPFTDTHRRAAEEQLGALGGGTWRYSMVVFTGVDKLPKGIFIEEHIANTGEALQWLVERCGSRYHAFDNTRKDSEDNTQVPELMEKVEEMITDNQGWYFEVNELILLEEEQARRALEEERMRMEEHARQRDQMIGGPPRELRLLLLGWKGVGKSSVGNSILGRRYFESGQETELCLRRQALVSGRRVTIVDTPGWDWFSVRRTPKRIRQESQRGAALLRPGPHTLLLVLPVVSSLTARKRRTLLAHIETLFGDSACLHTMVLFSCGDWLGRTPIEEHILRGGRELQRLLEYCGNYYHVLDSKTPGKDRSVSVLLDKIEEMIRENGDKAFLPIQTEWLSEESSYSSDNTEPEDDCRGCQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y7B8</accession>
    <name>A0A672Y7B8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Ceramide synthase 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cers6</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sphinganine + octadecanoyl-CoA = N-(octadecanoyl)-sphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:67033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36548"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y7B8"/>
    <dbReference type="FunCoup" id="A0A672Y7B8">
        <property type="interactions" value="1709"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002606.1">
        <property type="protein sequence ID" value="ENSSORP00005002542.1"/>
        <property type="gene ID" value="ENSSORG00005001516.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y7B8"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000020">
        <property type="entry name" value="Ceramide synthase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF43">
        <property type="entry name" value="CERAMIDE SYNTHASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="17">
        <location>
            <begin position="83"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="18">
        <location>
            <begin position="130"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="85"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="346"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="370"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049036"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="46201" checksum="F44B3BBFA16EC1CB" modified="2020-06-17" version="1">MAGILAWFWNERFWLPHNVTWADLKNTDEATFPQAEDLYLACPLAFCIFMIRLVFERFIARPCAMGLKIQANGPQKAQPNAILEKVFTAITKHPDEKRLEGLSKQLDWDVRTIQRWFRQRRNQEKPSTLTRFCESMWRFTFYLYIFTYGVRFLKKTPWLWNTKECWYNYPYQPLTVDIHYYYILELSFYLSLLFSQFTDIRRKDFLIMFLHHVATISLITFSYVNNMARVGTLVMCLHDAADVLIEAAKMANYAKCQILCNLLFAMFAILFISSRLGVYPVWILNTTLFESWEIVGPYPSWWVFNLLLILLQLLHSFWSYLIVKTACRAISKGKVGKWNPLHVSKDDRSDIESSSDEDDSPPPNQKHHSSTTNGTNKTHGTNGYLTGAPYPDEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCH1</accession>
    <name>A0A672YCH1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized LOC115437283</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115437283</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the gasdermin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016339.1">
        <property type="nucleotide sequence ID" value="XM_030160479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YCH1"/>
    <dbReference type="Ensembl" id="ENSSORT00005001384.1">
        <property type="protein sequence ID" value="ENSSORP00005001345.1"/>
        <property type="gene ID" value="ENSSORG00005000794.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437283"/>
    <dbReference type="InParanoid" id="A0A672YCH1"/>
    <dbReference type="OrthoDB" id="8445642at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040460">
        <property type="entry name" value="Gasdermin_pore"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041263">
        <property type="entry name" value="Gasdermin_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042377">
        <property type="entry name" value="GSDME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207:SF3">
        <property type="entry name" value="DEAFNESS, AUTOSOMAL DOMINANT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207">
        <property type="entry name" value="NONSYNDROMIC HEARING IMPAIRMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04598">
        <property type="entry name" value="Gasdermin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17708">
        <property type="entry name" value="Gasdermin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1210">Necrosis</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Gasdermin pore forming" evidence="12">
        <location>
            <begin position="63"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Gasdermin PUB" evidence="13">
        <location>
            <begin position="320"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="522" mass="58388" checksum="09A153360E8F0313" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFD5</accession>
    <name>A0A672YFD5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ornithine decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-ornithine + H(+) = putrescine + CO2</text>
            <dbReference type="Rhea" id="RHEA:22964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="4.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.17" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A672YFD5"/>
    <dbReference type="Ensembl" id="ENSSORT00005002616.1">
        <property type="protein sequence ID" value="ENSSORP00005002551.1"/>
        <property type="gene ID" value="ENSSORG00005001522.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFD5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="3 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="11">
        <location>
            <begin position="25"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="415" mass="46443" checksum="C141806FB2E0D3F3" modified="2025-10-08" version="2">MKTFVFDIPDIIYLHEVPFHVANLDDLHQKHIRWLRTLPRVKPFYAVKCNSTPAVIRMLSTLGTGFDCASKGEIELVLSLGVTPEKIIFAHTTKPQSHIKYASTRGVDMMTFDCEEELLKISACHTRAKLVLRIAVDDSKSAIKLSQKFGTKPSVVKKLLERCKELDLEVIGVSFHVGSGCTDSLAYRQAIEDARHVFDTAVLLGFQMRLLDIGGGFPGKEGFQIQFDEFSKVINEALDKFFPADSGVQVIAEPGRYYVDSAFTLAVNVFAKKVVMDDSSSDPENDRVMMYYISDGVFGSLNCLIYDPAHTNVAPYLHRKRYRSIIWGPTCDSLDRVTATYWMPEVQVGDWLLIDNMGAYSVCCSSDFSFFDKADIYPVLSADVVEAQAPCALSSHIYLSLPYLTLWVRGGAVHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGA2</accession>
    <name>A0A672YGA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RNA methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bcdin3d</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145; however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end 5'-phospho-ribonucleoside-RNA + 2 S-adenosyl-L-methionine = a 5'-end (5'-bismethylphospho)-ribonucleoside-RNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:58640"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="Rhea" id="RHEA-COMP:15182"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:142777"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5'-end 5'-phospho-ribonucleoside-RNA + S-adenosyl-L-methionine = a 5'-end (5'-methylphospho)-ribonucleoside-RNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:58656"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="Rhea" id="RHEA-COMP:15181"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:142776"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_029989326.1">
        <property type="nucleotide sequence ID" value="XM_030133466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YGA2"/>
    <dbReference type="FunCoup" id="A0A672YGA2">
        <property type="interactions" value="637"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003689.1">
        <property type="protein sequence ID" value="ENSSORP00005003587.1"/>
        <property type="gene ID" value="ENSSORG00005002154.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418915"/>
    <dbReference type="CTD" id="144233"/>
    <dbReference type="InParanoid" id="A0A672YGA2"/>
    <dbReference type="OrthoDB" id="273070at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000632">
        <property type="term" value="P:negative regulation of pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000138">
        <property type="entry name" value="BCDIN3 domain containing RNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010675">
        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF1">
        <property type="entry name" value="RNA 5'-MONOPHOSPHATE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="12">
        <location>
            <begin position="26"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="244" mass="27900" checksum="6CADC8583927DB65" modified="2020-06-17" version="1">MATCSTNSETVNEINDPGAAPYGNFINYYTFNPPENRLSLIPVTLLQDLGYNDHHQTTLLLDVGCNSGELSVAFYKHLVPSDLRTVHLLGFDLDESLIERAQQTNPLPGSISFIPLDITADTRQLQDYLRHHGCSHFHLCLCLAVTMWVHLNHGDSGLLQLLSHLASISDHLLLEAQPWKCYRSAARRLRKLGRSDFDHFKTLKIRGDMAEHAREHLERHCDMELIQSFGSTTWDRKLLLFRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ77</accession>
    <name>A0A672YJ77_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJ77"/>
    <dbReference type="Ensembl" id="ENSSORT00005004786.1">
        <property type="protein sequence ID" value="ENSSORP00005004650.1"/>
        <property type="gene ID" value="ENSSORG00005002831.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJ77"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003074">
        <property type="entry name" value="Immunoglobulin heavy variable 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="45"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="131" mass="14938" checksum="123EA3CF3193998C" modified="2020-06-17" version="1">MDFSLKHTVNMADRMRTFLVFAVLVLTGVQGWSITQSDPVVTRPGESHRLTCTATGLSFSSYYMGWIRQAPGKGLEWIAAIHYDSSEMYYSQSVQGRFTISRDNSKEQLYLQMNSLKTEDSAVYYCAREPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLI0</accession>
    <name>A0A672YLI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLI0"/>
    <dbReference type="Ensembl" id="ENSSORT00005005535.1">
        <property type="protein sequence ID" value="ENSSORP00005005378.1"/>
        <property type="gene ID" value="ENSSORG00005003188.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLI0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="10"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="115" mass="12730" checksum="045778A2C9C1661E" modified="2020-06-17" version="1">HVSNFTSVTKAAGKGGKKRRKSRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YM45</accession>
    <name>A0A672YM45_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Interferon regulatory factor 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">irf4a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YM45"/>
    <dbReference type="Ensembl" id="ENSSORT00005005913.1">
        <property type="protein sequence ID" value="ENSSORP00005005670.1"/>
        <property type="gene ID" value="ENSSORG00005003442.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YM45"/>
    <dbReference type="OrthoDB" id="8537190at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000041">
        <property type="entry name" value="Interferon regulatory factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019817">
        <property type="entry name" value="Interferon_reg_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001346">
        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019471">
        <property type="entry name" value="Interferon_reg_factor-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF6">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10401">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01243">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00601">
        <property type="entry name" value="IRF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="8">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="463" mass="53678" checksum="CE187E7F9E651737" modified="2020-06-17" version="1">MFYFPEMNMEEDTLSVSCGNGKLRQWLIDQIDSRRYAGLVWENDEKTIFRIPWKHAGKQDYNREEDAALFKAWALFKGKYKEGVDKPDPPTWKTRLRCALNKSNDFDELVDRSQLDISEPYKVYRIIPEGAKKGMKMSMEDTPSHLGYASPYSSLHNQVPSYMVTQERREWRDYSSPEQQSLPPSHHHGPHPELQYGQCHYPSPISRAWAGSHPENGFQLSFHTYLSESQPSVYTMDHSSPITDLSLHVSLFYRESLVKEVTTTSSEGCRITSSSSSSPSSSSSSSPCPEDRHPMGPEIILFPFPFPESHRQGAEMLPNILEKGVLLWMASDGMYAKRLCQGRVYWEGPLAPYMDKPNKLEKDQPCKLFDTQQFLIELQEFAHNGRHLPRHQIVLCFGDEYPDPQRTRKMITAQVEPVFARKLMYYYQQNNGHYLRAYDHIQEQNTSPVIDYPPQRPLQHIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQN1</accession>
    <name>A0A672YQN1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YQN1"/>
    <dbReference type="Ensembl" id="ENSSORT00005007158.1">
        <property type="protein sequence ID" value="ENSSORP00005006896.1"/>
        <property type="gene ID" value="ENSSORG00005003994.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YQN1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002962">
        <property type="entry name" value="Peropsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01244">
        <property type="entry name" value="PEROPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="58"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="276" mass="29916" checksum="7E63DFC81C52D3C3" modified="2020-06-17" version="1">MLLSNVSLSGCAGVNSALCAGAGEGHQGGGSFRITLSPTGNLIVAVCLGFIGTFGLLNNLLVLVLFCRYKMLRSPINLLLINISISDLLVCILGTPFSFAASTQGRWLIGKGGCMWYGFANSLFGIVSLISLAVLSYERYSTMIAPTEADSSNYRKISMGIVLTWVYSLVWTLPPLFGWSHYGPEGPGTTCSVDWTAKTPNNISYIICLFVFCLIVPFLVIVFCYGKLLYAIRQVGQSLLLYLNQTQQYSSVFQDVSIGHSKFGCFPNGTLSNLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YT90</accession>
    <name>A0A672YT90_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">N-acetylaspartate synthetase</fullName>
            <ecNumber evidence="14">2.3.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">N-acetyltransferase 8-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115426710</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-aspartate + acetyl-CoA = N-acetyl-L-aspartate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14165"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16953"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14166"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the NAT8 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.17" evidence="14"/>
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        <property type="protein sequence ID" value="ENSSORP00005007722.1"/>
        <property type="gene ID" value="ENSSORG00005004369.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YT90"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017188">
        <property type="term" value="F:L-aspartate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050769">
        <property type="entry name" value="NAT_camello-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947">
        <property type="entry name" value="GNAT FAMILY N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947:SF11">
        <property type="entry name" value="N-ACETYLASPARTATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
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    </dbReference>
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        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
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        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
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    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="20">
        <location>
            <begin position="108"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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            <dbReference type="ARBA" id="ARBA00039136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041029"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00043248"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="267" mass="29731" checksum="253EBC89EBCF8E2D" modified="2020-06-17" version="1">LSSSSNLPASIPLELHGVDQPYLVENTPIMDDDKLGKTEAHSIVVRSFEPSDKLEVQRIFYEGMMEMVSDTAFRGLRHHPESLLLYAAFTIGCLVITQCWWVIGLLPVSVLCARYFCSRRVIHAYLDCALQTDMGDVEGFYMKSPDSCLWVAVLEGKVVGLVAAVGHRTQTGTVELQRMSVDQNCRRRGVGMVLGRKVLEYAVTGGYSTVVLGTTAYNSAAHRLYQSLGFSCVGVTNGYTAPGTRQSVLERVFYRVRHHHYSLDEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYR2</accession>
    <name>A0A672YYR2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atf7ip</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCAF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030003462.1">
        <property type="nucleotide sequence ID" value="XM_030147602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YYR2"/>
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        <property type="interactions" value="992"/>
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        <property type="protein sequence ID" value="ENSSORP00005009649.1"/>
        <property type="gene ID" value="ENSSORG00005005267.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A672YYR2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATF7-int"/>
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        <property type="entry name" value="ATF7IP_BD"/>
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        <property type="entry name" value="Fn3_ATF7IP"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23210">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 7 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23210:SF26">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 7-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16788">
        <property type="entry name" value="ATF7IP_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16794">
        <property type="entry name" value="fn3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="838"/>
            <end position="944"/>
        </location>
    </feature>
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            <begin position="31"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="669"/>
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        </location>
    </feature>
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            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
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            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
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            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
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        </location>
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            <end position="150"/>
        </location>
    </feature>
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            <end position="172"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
            <begin position="250"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
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        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
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        <location>
            <begin position="669"/>
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        </location>
    </feature>
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        <location>
            <begin position="711"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="743"/>
            <end position="760"/>
        </location>
    </feature>
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        <location>
            <begin position="774"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="808"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="818"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="947" mass="99445" checksum="2E63853A4E09B798" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZP8</accession>
    <name>A0A672YZP8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="4 10">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ugp2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
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        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
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        <location>
            <position position="139"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
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            <position position="202"/>
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        <ligand>
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            <position position="234"/>
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        <ligand>
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        <location>
            <position position="235"/>
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        <ligand>
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        <ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023579"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    <sequence length="520" mass="58567" checksum="C4559B615C3C86CF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0V4</accession>
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        </submittedName>
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        <name evidence="12" type="primary">c16h1orf43</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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            <begin position="20"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="318" mass="36901" checksum="BAA30970E4B439E6" modified="2020-06-17" version="1">MWDILNHWLVQCCSSLCRCSLLSLFLFVFLLLFFSFRWIRFVRYCLLLLLLLLLLLLLLLLLLLSLHIAFKYNLITLLLVFVLLFIFVKRQIMRFAMKSRRGPHVPLGHNAPKELRQEIEAKLCQVQKIHFEPRLLSPDDDRLKHRDQSDSCDYLYRMRALDAIRDTDLPFRELGGTSTAVTGKRFRTWLLQLRKSHCLFRNSQDKTSSLIDTVLDGYNKARHGSEAFGEAEFLKYQEALTELASVVKSHSSSSNTLSQHHQTAAKDLTITTEPASPSTSPTQTTYLTSTAQQRSKRSRHFLELKNFKDNYNTLDSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z199</accession>
    <name>A0A672Z199_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">scpep1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in vascular wall and kidney homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z199"/>
    <dbReference type="Ensembl" id="ENSSORT00005011315.1">
        <property type="protein sequence ID" value="ENSSORP00005010941.1"/>
        <property type="gene ID" value="ENSSORG00005005043.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z199"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
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        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF3">
        <property type="entry name" value="RETINOID-INDUCIBLE SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025719503" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="445" mass="49385" checksum="BCC5FBCB0CC37EBF" modified="2020-06-17" version="1">MGRFQTSAVLFFFGFLLHKGLPSPGSGTTEVWDYVEVRPGAHMFWWLYSADSPDLPLVMWLQGGPGGSGSGFGNFEEIGPLNRDLQPRNTSWVQAASVLFVDNPVGTGFSYTERPDGFATDVATVASDMLVLLQHFFTKQAEFQSVPFYIFSESYGGKMAAVISLELTKAITKGTVKCNFAGVALGDSWISPLDSVMTWGPYLYVTSLLDDYGLADVTKAAEAVKEAVNNKQFLKATELWSVAETVVEQNTNGVNFYNILTQEPDDKRTKSSPEGDFILTMAFPLVLQIRRHIQRLHSLSLSELMNGPIRKKLGIIPQNVTWGGQAEEVFSNMAGDFMRPVVDTVDQLLTAGVNVTVYNGQLDLIVDTMGQELWVKRLKWEGLPFFNKLRWTALDDPASPGATGAFYKNYKNFGFYWILKAGHMIPSDQGPMALQMLKMITQQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z368</accession>
    <name>A0A672Z368_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC115418324</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="FunCoup" id="A0A672Z368">
        <property type="interactions" value="946"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012046.1">
        <property type="protein sequence ID" value="ENSSORP00005011661.1"/>
        <property type="gene ID" value="ENSSORG00005006231.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z368"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000068">
        <property type="entry name" value="Desmoglein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000083">
        <property type="entry name" value="Desmoglein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000074">
        <property type="entry name" value="Desmoglein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009122">
        <property type="entry name" value="Desmosomal_cadherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF1">
        <property type="entry name" value="DESMOGLEIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01818">
        <property type="entry name" value="DESMOCADHERN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="95"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="177"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="290"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="539"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="378"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="442"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1026"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="390"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="442"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1041"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1062"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1372" mass="144609" checksum="E6C1257D40599BE5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5I6</accession>
    <name>A0A672Z5I6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115426494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000498.1">
        <property type="nucleotide sequence ID" value="XM_030144638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z5I6"/>
    <dbReference type="FunCoup" id="A0A672Z5I6">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012958.1">
        <property type="protein sequence ID" value="ENSSORP00005012561.1"/>
        <property type="gene ID" value="ENSSORG00005006589.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426494"/>
    <dbReference type="InParanoid" id="A0A672Z5I6"/>
    <dbReference type="OrthoDB" id="432835at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF39">
        <property type="entry name" value="LEUKOCYTE SURFACE ANTIGEN CD53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="229" mass="25112" checksum="0EBD16ED6F7D14CF" modified="2020-06-17" version="1">MAQGCLKCLKYTMCVANFLCFICGISVLGLGIYMILTFKMAALLPTLASFNFANILVITGIIITCVSFLGFLGALKENRCLLLTFFLLLFILMLVELAAACLLLLYEDSIAGWVEEDLKNGLKDAKKNPGNSTLTEAWNFVQKNLKCCGVTNSSDWGAHVPESCCVQTCTTPPKYFTEGCFGTLKNFFEENFLSTGIAVIVLCIIEVLGMCFAMTLFCHISRSGLGYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z648</accession>
    <name>A0A672Z648_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SH3 and PX domain-containing protein 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">sh3pxd2aa</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH3PXD2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z648"/>
    <dbReference type="FunCoup" id="A0A672Z648">
        <property type="interactions" value="1116"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012686.1">
        <property type="protein sequence ID" value="ENSSORP00005012289.1"/>
        <property type="gene ID" value="ENSSORG00005006486.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z648"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12074">
        <property type="entry name" value="SH3_Tks5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12077">
        <property type="entry name" value="SH3_Tks5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12019">
        <property type="entry name" value="SH3_Tks5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12020">
        <property type="entry name" value="SH3_Tks5_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000020">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000031">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000042">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000059">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000141">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035450">
        <property type="entry name" value="SH3PXD2A_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035452">
        <property type="entry name" value="SH3PXD2A_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035453">
        <property type="entry name" value="SH3PXD2A_SH3_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035454">
        <property type="entry name" value="SH3PXD2A_SH3_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="105"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="205"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="408"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="791"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="1050"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="467"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="550"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="608"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="943"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="987"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="611"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="758"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="958"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1111" mass="122988" checksum="85887D4B41912D42" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z789</accession>
    <name>A0A672Z789_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipid scramblase CLPTM1L</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cisplatin resistance-related protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cleft lip and palate transmembrane protein 1-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Scramblase that mediates the translocation of glucosaminylphosphatidylinositol (alpha-D-GlcN-(1-6)-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol, GlcN-PI) across the endoplasmic reticulum (ER) membrane, from the cytosolic leaflet to the luminal leaflet of the ER membrane, where it participates in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a lipid glycoconjugate involved in post-translational modification of proteins. Can also translocate 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) (phosphatidylinositol or PI), as well as several other phospholipids (1,2-diacyl-sn-glycero-3-phosphocholine, 1,2-diacyl-sn-glycero-3-phosphoethanolamine), and N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI) in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = 6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71495"/>
            <dbReference type="ChEBI" id="CHEBI:190691"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71491"/>
            <dbReference type="ChEBI" id="CHEBI:57997"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z789"/>
    <dbReference type="Ensembl" id="ENSSORT00005013126.1">
        <property type="protein sequence ID" value="ENSSORP00005012724.1"/>
        <property type="gene ID" value="ENSSORG00005006657.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z789"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="301"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="450" mass="51789" checksum="69CCA8540596A431" modified="2020-06-17" version="1">LFPSSSLIDSIAKLLLGVFVVYMLHTAWLLYGFLNTKPCDGGEHCITSYLAARPRLQVNQHLHINNKRQVKVYIPEETRSNGSLYAVVYMHKAGVSPLDDSREVHYAAQLTTYIPPRHTEGQRNSNKVKCSLYSLKVPTRPDRPISHWRPHLSITVMSEAFTFNKAGLPSDVRRYMRVSQEGKQMMYLPLLLVNELSFRVKDLMEISIDTDYLPLTVSYEGISLRRFRFWIHLQDVVYSLRQFGFTEENIDEIKETLADSNLYLLVLTAVITLICEFLALKNDISSWRKKKNMAGMSRKSVLWRSLGTLLIFLHLLEETSLLVLLPVGLGACVEVHKGIVLFNSFSLIYIGVCGSDQVNKLDEEERKTVEYDSQVRAFFCWNLLHFVSVFVVSWCNLNDFLFRLPDTCPTWSILCVSVELFSHWPIYARRGEIITLYLTFCSRLYSSLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z871</accession>
    <name>A0A672Z871_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Retinaldehyde binding protein 1a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115417134</name>
        <name evidence="3" type="synonym">LOC115417133</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z871"/>
    <dbReference type="Ensembl" id="ENSSORT00005013413.1">
        <property type="protein sequence ID" value="ENSSORP00005013005.1"/>
        <property type="gene ID" value="ENSSORG00005006782.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013420.1">
        <property type="protein sequence ID" value="ENSSORP00005013012.1"/>
        <property type="gene ID" value="ENSSORG00005006784.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alpha-tocopherol transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF233">
        <property type="entry name" value="CELLULAR RETINALDEHYDE-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="133"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="310" mass="35633" checksum="C63CCBE8123D8C9C" modified="2020-06-17" version="1">MTGSFRMVSEDEQAMRSKLEHLTVKDHGPVFGPCHKLPGHTVQKAKDELNETDERRASSLKDLRTMMKDKAAEGNDLAKLVLERFGDQPDAFLLRFLRARKFDVARSHELMKGYVRFRKEYPELFENLTVEAVRSTIEAGYPVVLPSRDKYGRAVLLFNIENWDLEEITFDEILRAYCVILEKLLENEETQINGFVLIENFKGFTMQHASGIKPAELKKMVDMLQDSFPARFKAVHVTHQPWYFTTTYNVVKPFMKSKLLERVFVHGDELDSYFKEFDADILPADFDGKASVSDCQAIATKLFGSEDTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9Z1</accession>
    <name>A0A672Z9Z1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">spaca4l</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SPACA4/bouncer family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z9Z1"/>
    <dbReference type="FunCoup" id="A0A672Z9Z1">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013727.1">
        <property type="protein sequence ID" value="ENSSORP00005013317.1"/>
        <property type="gene ID" value="ENSSORG00005006906.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9Z1"/>
    <dbReference type="OrthoDB" id="5962859at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035036">
        <property type="term" value="P:sperm-egg recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046354">
        <property type="entry name" value="SPACA4/Bouncer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47613">
        <property type="entry name" value="SPERM ACROSOME MEMBRANE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47613:SF1">
        <property type="entry name" value="SPERM ACROSOME MEMBRANE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025408300" description="UPAR/Ly6 domain-containing protein" evidence="10">
        <location>
            <begin position="21"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="11">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="13657" checksum="AD3C3B1597082B67" modified="2020-06-17" version="1">MNRLILQIFALGFCFAIVHTLKCYNCKIGFFDVCFTTETTCASGEHCFSGTGKAVGFVDIMMKGCLAEAECNQTSEVNFPSDSNTTAYTMTKTCCNTDLCNSAPGLPGGSGLKLALASVAALFVAHLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBN6</accession>
    <name>A0A672ZBN6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZBN6"/>
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        <property type="gene ID" value="ENSSORG00005007132.1"/>
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    <dbReference type="InParanoid" id="A0A672ZBN6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025395586" evidence="2">
        <location>
            <begin position="23"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="191" mass="20703" checksum="A29CB23EB09ACD91" modified="2020-06-17" version="1">MKTDALLFILLLLKHTAVLILAPPPYTGTPQAPPPRTETPQAPHPCTETPQAPPPCTETPQAPPPWTEPPQAPPPWTETPHAPPPWTETPQAPPPWTETPHAPPPWTEPPQAPPPWTETPQVPPPWTEPPQAPPPWTETPHAPPPWTEPPQAPPPCTETPQAPPPCTETPQAPPPWTEPPQAPPPCTETPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZD35</accession>
    <name>A0A672ZD35_9TELE</name>
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        <recommendedName>
            <fullName evidence="6 16">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="16">Aconitase</shortName>
            <ecNumber evidence="5 16">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115423345</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="16">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="5 16"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZD35"/>
    <dbReference type="Ensembl" id="ENSSORT00005014811.1">
        <property type="protein sequence ID" value="ENSSORP00005014388.1"/>
        <property type="gene ID" value="ENSSORG00005006814.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZD35"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="AcnA_Mitochon_Swivel"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
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    </dbReference>
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        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="18">
        <location>
            <begin position="81"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="19">
        <location>
            <begin position="596"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="530"/>
            <end position="571"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="793" mass="85927" checksum="9EC8A3C8D653ACA8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZD95</accession>
    <name>A0A672ZD95_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Myocardial zonula adherens protein-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">myzap</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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        </lineage>
    </organism>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051375">
        <property type="entry name" value="Tuftelin_GRINL1A/MYZAP/CCD68"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23171:SF3">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23171">
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    </dbReference>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="581"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="218"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="498"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="620"/>
            <end position="634"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="652" mass="74640" checksum="F8AD06E4D9DD45EA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDS7</accession>
    <name>A0A672ZDS7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic translation initiation factor 4E-1A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115426874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDS7"/>
    <dbReference type="FunCoup" id="A0A672ZDS7">
        <property type="interactions" value="1516"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015551.1">
        <property type="protein sequence ID" value="ENSSORP00005015090.1"/>
        <property type="gene ID" value="ENSSORG00005007646.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDS7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF74">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="217" mass="25151" checksum="A92780ED4EF6C275" modified="2020-06-17" version="1">MRTSRWCQVITFAGNPEKEKSEKATQKVVSPNVYIKHPLQNKWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMSGCDYSLFKDGIEPMWEDERNRRGGRWLITLSKQQRKCDLDRFWLETLLCLVGEAFDDYSDDVCGAVINVRAKGDKIAIWTTDYENKEAITHIGRVYKERLGVPPRVIIGYQSHADTATKSGSSTKNKFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFJ6</accession>
    <name>A0A672ZFJ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZFJ6"/>
    <dbReference type="Ensembl" id="ENSSORT00005016092.1">
        <property type="protein sequence ID" value="ENSSORP00005015599.1"/>
        <property type="gene ID" value="ENSSORG00005007918.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZFJ6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001155">
        <property type="entry name" value="Zinc finger 30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000247">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF139">
        <property type="entry name" value="PAIR-RULE PROTEIN ODD-PAIRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="324"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="40938" checksum="3C002E1257850082" modified="2020-06-17" version="1">MKNKQHFPSTPVPVKCEDDEFNQTQTEDNKMEANKVDCGGSEPASSFHPHRHLQTKTEEQIEDSEETEDSDEDQTLNHKPESKLPTNSSIEQMETETDGDDSGGSEPDMNPVHMESVKRRTFDSKSLLEEHMNTHTGQKLVGSSECEATFSQKTNTIQETNSPRDMMTNNDLPLSDVGCEKKLHHCSVCSKTFCWKSHLEIHMRIHTGEKPFSCSLCGSRFKHKSSLCSHMTSHTREKTFRCSLCHKCFRDRSEVRRHMTIHTGEKVFSCSLCHKCFTDQSEVRRHMRVHTGERPFSCSECSKRFTWKTDLQRHMRVHTGERPFCCVVCQKRFTRKYHLHFHHVSFHGTTDSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZH04</accession>
    <name>A0A672ZH04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Solute carrier family 7 member 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">slc7a7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZH04"/>
    <dbReference type="Ensembl" id="ENSSORT00005016853.1">
        <property type="protein sequence ID" value="ENSSORP00005016345.1"/>
        <property type="gene ID" value="ENSSORG00005007753.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZH04"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000821">
        <property type="term" value="P:regulation of arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000003">
        <property type="entry name" value="Y+L amino acid transporter 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF303">
        <property type="entry name" value="Y+L AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="55518" checksum="817FBAEF02ED0A53" modified="2020-06-17" version="1">MASSTTSEVTNGDPKSKGSEESMKLKKEISLLNGVCLIVGNMIGSGIFVSPKGVLIHSASYGLSLVVWTVGGIFSVFGALCYAELGTTITKSGASYAYILEAFGGFLAFIRLWTSLLIIEPTSQAIIAITFSNYMVQPIFPTCIAPYLANRLLAAACICLLTFVNCTYVKWGTRVQDFFTYAKVIALIAVIITGLVKIGQGHTQNFEGLFDGSSQDPGDLALALYSALFSYSGWDTLNFVTEEIKNPERNLPLAIAISMPIVTVIYILTNVAYYTILPINAILDSDAVAVTFADKVFGVMNWTIPLAVALSCFGGLNASILASSRLFFVGSREGHLPDYLCMIHVTRYTPIPALLFNGVMALIYLCVEDVFRLINYYSFSYWFFVGLSILGQLYLRWKQPNRKRPLKLSLFYPIVFCFLTVFLVVVPLYSDTINSLIGIGIALSGVPVYFLCCYAPAHKRPLWWAAAAIPYWEQCLAQGHFDMWTIGARIQTADPYVIGRLALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZH94</accession>
    <name>A0A672ZH94_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZH94"/>
    <dbReference type="Ensembl" id="ENSSORT00005017146.1">
        <property type="protein sequence ID" value="ENSSORP00005016636.1"/>
        <property type="gene ID" value="ENSSORG00005008415.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZH94"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15518" checksum="3CDDCB50EBB670C7" modified="2020-06-17" version="1">MRKNLTLTVQMASNVTGMTRRSHLRCFLPGTVEGGSIMIWGAFSFSGTLELQVVQGRQTAAGYVQMLQRASLMTEGPRLCGNSWVFQQDNAAVHNARLTKDFFRENNITLLDHSLLCRCGRWIWSCGSCLYAIAMFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHV8</accession>
    <name>A0A672ZHV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rad21/Rec8-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZHV8"/>
    <dbReference type="Ensembl" id="ENSSORT00005016558.1">
        <property type="protein sequence ID" value="ENSSORP00005016058.1"/>
        <property type="gene ID" value="ENSSORG00005008110.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZHV8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF54">
        <property type="entry name" value="RAD21 COHESIN COMPLEX COMPONENT LIKE 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="130" mass="14848" checksum="14937614603CCACB" modified="2020-06-17" version="1">MFYTQLFTSKRGHLAKIWLAAHWERKLTKAHVFECNLENTVRDIISTKMKIGLRTSGHLLLGVVRVYSRKAKYLLADCNDALVKIKMAFRPGQTDLPLEGLEATLKAITLIEDFTDFDTQLPHPRYGCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI06</accession>
    <name>A0A672ZI06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">DNA topoisomerase 2</fullName>
            <ecNumber evidence="18">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">top2a</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17 18">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 18">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="18"/>
    <dbReference type="FunCoup" id="A0A672ZI06">
        <property type="interactions" value="904"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016613.1">
        <property type="protein sequence ID" value="ENSSORP00005016108.1"/>
        <property type="gene ID" value="ENSSORG00005001902.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZI06"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:rhythmic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:sister chromatid segregation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="HATPase_TopII-like"/>
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        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
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        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DNA topoisomerase 2"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="Topoisomerase II, domain 5"/>
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        <property type="entry name" value="DNA_Topoisomerase_II"/>
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        <property type="entry name" value="HATPase_C_sf"/>
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        <property type="entry name" value="HATPase_dom"/>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
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        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
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        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
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        <property type="entry name" value="Topo_IIA_A/C_ab"/>
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        <property type="entry name" value="Topo_IIA_B_C"/>
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        <property type="entry name" value="Type II DNA topoisomerase"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
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    </dbReference>
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    <keyword evidence="13" id="KW-0090">Biological rhythms</keyword>
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    <keyword evidence="14 17 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15 17 18" id="KW-0413">Isomerase</keyword>
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    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="426"/>
            <end position="543"/>
        </location>
    </feature>
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        <location>
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            <end position="1132"/>
        </location>
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            <end position="1084"/>
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        <location>
            <begin position="1228"/>
            <end position="1360"/>
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        <location>
            <begin position="1068"/>
            <end position="1077"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1241"/>
            <end position="1255"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1257"/>
            <end position="1267"/>
        </location>
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        <location>
            <position position="776"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
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        </source>
    </evidence>
    <sequence length="1374" mass="156026" checksum="19169E0D0BDD275C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZI20</accession>
    <name>A0A672ZI20_9TELE</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="8" type="primary">LOC115433600</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 136"/>
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    </dbReference>
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        <property type="entry name" value="TRAM_LAG1_CLN8"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
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        <location>
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        <location>
            <begin position="104"/>
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        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
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        <location>
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            <end position="209"/>
        </location>
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            <begin position="26"/>
            <end position="217"/>
        </location>
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        </source>
    </evidence>
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    </evidence>
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        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="246" mass="27496" checksum="20C8AC8DB59102E1" modified="2020-06-17" version="1">MPVLEVFCSLIGWLCLYLLFGSAFPLRGPEWNCRLVTLTHGLAIVMTTAYVVFIDGPWPFTHAGTANTPLQTLSLSVCLGYFFFDLGWCVWFRSEGPLMMAHHGASILGILLALLMGVSGCETCAVIFGSELTNPLLQSRWFLRQLSMYDGLLGDAVDLLFILLFATVRVGVGTAMFYCELTSPRTPLVMKLGGVVMYVLAWVFMVDIARFGYKKSKFFCGYVFVHAIPHLSSRHRQSLLVIYKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJW6</accession>
    <name>A0A672ZJW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZJW6"/>
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        <property type="protein sequence ID" value="ENSSORP00005016773.1"/>
        <property type="gene ID" value="ENSSORG00005008481.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJW6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000733">
        <property type="entry name" value="Zinc finger protein 236 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="338" mass="38941" checksum="E01BE341531E1493" modified="2020-06-17" version="1">MEPIEDDEFIKTEVEEHNGELQYSDNHNLSKLELLRYYPETDMDPTEDLLPEEATQGENNSPPVPAEDFLEARDNFLRCPFCPKTFSRATSLNIHIKSHTGEKAHSCSYCGKRFSRADLLKSHKRTHTGERPYSCSICSKTYHNQLKVHLRTHTGERPYSCQECSKTFSNAGNLRIHERIHTGEKPYCCAQCGKRFNGLGDLKTHYRIHTGERPYSCELCKKTFSQAGHLTIHMRMHTGERPYSCNECGKKFTVASSLKLHLRTHTGEKEYSCSYCNKSFSRSGHLKRHELVHTKEKVYLCSQCGKTYTDQSSLKKHLKMHAAKDQHSCLVCLILQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVK3</accession>
    <name>A0A672ZVK3_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Golgin subfamily A conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">golga2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030004400.1">
        <property type="nucleotide sequence ID" value="XM_030148540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZVK3"/>
    <dbReference type="Ensembl" id="ENSSORT00005020849.1">
        <property type="protein sequence ID" value="ENSSORP00005020278.1"/>
        <property type="gene ID" value="ENSSORG00005009824.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GOLGA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043976">
        <property type="entry name" value="GOLGA_cons_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10881:SF46">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 2"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10881">
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    </dbReference>
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        <property type="entry name" value="GM130_C"/>
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    </dbReference>
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        <location>
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            <end position="975"/>
        </location>
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        <location>
            <begin position="936"/>
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        <location>
            <begin position="43"/>
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        <location>
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        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
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        <location>
            <begin position="826"/>
            <end position="844"/>
        </location>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF15070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1100" mass="124517" checksum="5483CF4E54162419" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVQ1</accession>
    <name>A0A672ZVQ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small acidic protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="primary">LOC115421223</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
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            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZVQ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005021822.1">
        <property type="protein sequence ID" value="ENSSORP00005021186.1"/>
        <property type="gene ID" value="ENSSORG00005010343.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021823.1">
        <property type="protein sequence ID" value="ENSSORP00005021187.1"/>
        <property type="gene ID" value="ENSSORG00005010343.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021824.1">
        <property type="protein sequence ID" value="ENSSORP00005021188.1"/>
        <property type="gene ID" value="ENSSORG00005010343.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10066125at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026714">
        <property type="entry name" value="SMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028124">
        <property type="entry name" value="SMAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22175:SF0">
        <property type="entry name" value="SMALL ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22175">
        <property type="entry name" value="SMALL ACIDIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15477">
        <property type="entry name" value="SMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small acidic protein-like" evidence="4">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="175" mass="19310" checksum="D11D20FF76C8BC26" modified="2020-06-17" version="1">MSSTEDRHGTKRPASPTEDGSTQWASADLGSDERKQKFLRLMGAGKKEHTGRLVIGDHKSTSHVRSGLEDRHINQQLEQQYQQGMDGKLSGRNRRHCGLGFSESEPDPPPSAPVDTQTTPADPHETDSDKDQTGDQNQSSDPEGPDLTSDQPKPASDSRTDQRKHTYKMAFVKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZYD3</accession>
    <name>A0A672ZYD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EEF1A lysine methyltransferase 2</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Methyltransferase-like protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Protein-lysine N-methyltransferase METTL10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">eef1akmt2</name>
        <name evidence="7" type="synonym">EEF1AKMT2</name>
        <name evidence="7" type="synonym">METTL10</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-318'.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:54193"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM4 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_030011658.1">
        <property type="nucleotide sequence ID" value="XM_030155798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZYD3"/>
    <dbReference type="FunCoup" id="A0A672ZYD3">
        <property type="interactions" value="1431"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022143.1">
        <property type="protein sequence ID" value="ENSSORP00005021497.1"/>
        <property type="gene ID" value="ENSSORG00005010525.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434102"/>
    <dbReference type="CTD" id="399818"/>
    <dbReference type="InParanoid" id="A0A672ZYD3"/>
    <dbReference type="OrthoDB" id="540004at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000172">
        <property type="entry name" value="EEF1A lysine methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03188">
        <property type="entry name" value="Methyltr_EFM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026635">
        <property type="entry name" value="Efm4/METTL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843:SF5">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12843">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE METTL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="9">
        <location>
            <begin position="77"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="235" mass="26259" checksum="3F7A1E4061680577" modified="2020-06-17" version="1">MEDGNESPHGSTSEPEEDRCSEEDFGPSKLGTKEYWEDAYEKELETFKDIGDVGEIWFGEESMSRVLRWMDKAKIAENAAILDIGTGNAVFLVELAKHGYKNLMGIDYSAASVELARNVLLAEDLMDVTVKEMDFLNGQGELKGFDVCIDKGTFDAISLNPDNAKEGKRLYVEALKDALKDKGFFSITSCNWTKEQLLERFSEGFEFVEELPTPSFKFGGKSGNSVTALIFKRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A100</accession>
    <name>A0A673A100_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 26">Lipoprotein lipase</fullName>
            <shortName evidence="26">LPL</shortName>
            <ecNumber evidence="4 26">3.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">lpla</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 26">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.34"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="26">Homodimer. Interacts with APOC2; the interaction activates LPL activity in the presence of lipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="26">Cell membrane</location>
            <topology evidence="26">Peripheral membrane protein</topology>
            <orientation evidence="26">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 26">Secreted</location>
            <location evidence="2 26">Extracellular space</location>
            <location evidence="2 26">Extracellular matrix</location>
        </subcellularLocation>
        <text evidence="26">Newly synthesized LPL binds to cell surface heparan proteoglycans and is then released by heparanase. Subsequently, it becomes attached to heparan proteoglycan on endothelial cells. Locates to the plasma membrane of microvilli of hepatocytes with triglyceride-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.</text>
    </comment>
    <comment type="PTM">
        <text evidence="26">Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 25">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.34" evidence="4 26"/>
    <dbReference type="AlphaFoldDB" id="A0A673A100"/>
    <dbReference type="Ensembl" id="ENSSORT00005023067.1">
        <property type="protein sequence ID" value="ENSSORP00005022407.1"/>
        <property type="gene ID" value="ENSSORG00005010908.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A100"/>
    <dbReference type="OrthoDB" id="199913at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042627">
        <property type="term" value="C:chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034185">
        <property type="term" value="F:apolipoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034372">
        <property type="term" value="P:very-low-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000024">
        <property type="entry name" value="Lipoprotein lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000031">
        <property type="entry name" value="Lipoprotein lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002330">
        <property type="entry name" value="Lipo_Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03230">
        <property type="entry name" value="lipo_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF3">
        <property type="entry name" value="LIPOPROTEIN LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00822">
        <property type="entry name" value="LIPOLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 26" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 26" id="KW-0162">Chylomicron</keyword>
    <keyword evidence="19 26" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 26" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="13 26" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 26" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="17 26" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 26" id="KW-0944">Nitration</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 26" id="KW-0964">Secreted</keyword>
    <keyword evidence="12 26" id="KW-0732">Signal</keyword>
    <keyword evidence="21 26" id="KW-0850">VLDL</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025708357" description="Lipoprotein lipase" evidence="26">
        <location>
            <begin position="25"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="27">
        <location>
            <begin position="363"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="22">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU362020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="515" mass="58072" checksum="6273FE59C3E13154" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2X7</accession>
    <name>A0A673A2X7_9TELE</name>
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        <recommendedName>
            <fullName evidence="11">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115430998</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030007086.1">
        <property type="nucleotide sequence ID" value="XM_030151226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A2X7"/>
    <dbReference type="Ensembl" id="ENSSORT00005023649.1">
        <property type="protein sequence ID" value="ENSSORP00005022978.1"/>
        <property type="gene ID" value="ENSSORG00005011132.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430998"/>
    <dbReference type="InParanoid" id="A0A673A2X7"/>
    <dbReference type="OrthoDB" id="1844at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="450" mass="50344" checksum="172FA7868446B818" modified="2020-06-17" version="1">MRECISIHVGQAGVQMGNTCWELYCLEHGIQPDGHMPSDKPAGGYDDSFTTFFSETGTGKYVPRAIFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTVGKEHIDSVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDFGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNSCFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNVAISNIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSFEGDEEGEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5R7</accession>
    <name>A0A673A5R7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Low molecular weight neuronal intermediate filament-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">neff1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A5R7"/>
    <dbReference type="FunCoup" id="A0A673A5R7">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024696.1">
        <property type="protein sequence ID" value="ENSSORP00005023987.1"/>
        <property type="gene ID" value="ENSSORG00005011526.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A5R7"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006821">
        <property type="entry name" value="Intermed_filament_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF13">
        <property type="entry name" value="NEUROFILAMENT LIGHT POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04732">
        <property type="entry name" value="Filament_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="95"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="434"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="99"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="441"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="499"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="523" mass="59992" checksum="6BF7A7AE547EEC29" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A9N0</accession>
    <name>A0A673A9N0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="22">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">gapdh</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 22">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-nitroso-L-cysteinyl-[GAPDH] + L-cysteinyl-[protein] = L-cysteinyl-[GAPDH] + S-nitroso-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:17089"/>
            <dbReference type="Rhea" id="RHEA-COMP:17090"/>
            <dbReference type="Rhea" id="RHEA-COMP:17091"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:149494"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66685"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 22">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="22"/>
    <dbReference type="AlphaFoldDB" id="A0A673A9N0"/>
    <dbReference type="FunCoup" id="A0A673A9N0">
        <property type="interactions" value="644"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026049.1">
        <property type="protein sequence ID" value="ENSSORP00005025292.1"/>
        <property type="gene ID" value="ENSSORG00005010880.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A9N0"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000319">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF111">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12 22" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 19 22" id="KW-0520">NAD</keyword>
    <keyword evidence="19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="23">
        <location>
            <begin position="13"/>
            <end position="156"/>
        </location>
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            <position position="156"/>
        </location>
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        <location>
            <begin position="17"/>
            <end position="18"/>
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        <location>
            <position position="39"/>
        </location>
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            <position position="84"/>
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        <location>
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        </ligand>
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        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
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        </ligand>
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        <location>
            <position position="186"/>
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        <ligand>
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            <begin position="215"/>
            <end position="216"/>
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        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
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        <location>
            <position position="238"/>
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            <name>D-glyceraldehyde 3-phosphate</name>
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        </ligand>
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        <location>
            <position position="183"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
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        <source>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00047698"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048005"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000397"/>
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        <source>
            <dbReference type="RuleBase" id="RU361160"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025292.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="339" mass="36777" checksum="642EE9C8F09629E1" modified="2020-06-17" version="1">MSTKAGKIKTFLIQFGRIGRLVTRAAFHSKVVEIVAINDPFIDLEYMVYMFQYDSTHGRFKGEVKVEGDKLVIDGHKITVFHERDPANIKWGDAGAQYVVESTGVFTTIEKASLHLKGGAKRVIISAPSADAPMFVMGVNHEKYNNTLQVVSNASCTTNCLAPLAKVINDNFGIIEGLMSTVHAITATQKTVDGPSGKLWRDGRGASQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTVRLEKPAKYDDIKKVVKDAAEGPLKGILGYTDHQVVSTDFNGDCHSSIFDAGAGIALNDHFVKLVSWYDNEFGYSNRVCDLMAHMASKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AC34</accession>
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        <submittedName>
            <fullName evidence="9">Rho GTPase-activating protein 15-like</fullName>
        </submittedName>
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        <name evidence="9" type="primary">arhgap27</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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    <reference evidence="9" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 27"/>
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        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
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            <begin position="293"/>
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        </location>
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        <location>
            <begin position="488"/>
            <end position="521"/>
        </location>
    </feature>
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        <location>
            <begin position="593"/>
            <end position="707"/>
        </location>
    </feature>
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        <location>
            <begin position="796"/>
            <end position="984"/>
        </location>
    </feature>
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            <begin position="62"/>
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        </location>
    </feature>
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        </location>
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    </feature>
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            <begin position="734"/>
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    </feature>
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        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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            <end position="140"/>
        </location>
    </feature>
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            <begin position="225"/>
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        </location>
    </feature>
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            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
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        <location>
            <begin position="572"/>
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        <location>
            <begin position="734"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="986" mass="111465" checksum="D6B233BCB238D2A6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACK4</accession>
    <name>A0A673ACK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ERVV2 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ACK4"/>
    <dbReference type="Ensembl" id="ENSSORT00005028096.1">
        <property type="protein sequence ID" value="ENSSORP00005027310.1"/>
        <property type="gene ID" value="ENSSORG00005013039.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ACK4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018154">
        <property type="entry name" value="TLV/ENV_coat_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424:SF73">
        <property type="entry name" value="ENDOGENOUS RETROVIRUS GROUP FC1 ENV POLYPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424">
        <property type="entry name" value="VIRAL ENVELOPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00429">
        <property type="entry name" value="TLV_coat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58069">
        <property type="entry name" value="Virus ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13831" checksum="F96269B940E6D2BA" modified="2020-06-17" version="1">MWGLPKLAHYIDDIAVDLEDVTKSMTEGFSAVSAEIKALKNVALQNRAALDYMLASSGGVCHIIGQECCTFIPDVSGNLTYVVDHLNELLIRLTFCPVSIWHSFFHLATVSYVIFYYIILTNPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHS1</accession>
    <name>A0A673AHS1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SLIT-ROBO Rho GTPase activating protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AHS1"/>
    <dbReference type="Ensembl" id="ENSSORT00005029699.1">
        <property type="protein sequence ID" value="ENSSORP00005028881.1"/>
        <property type="gene ID" value="ENSSORG00005013789.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AHS1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000006">
        <property type="entry name" value="SLIT-ROBO Rho GTPase-activating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051627">
        <property type="entry name" value="SLIT-ROBO_RhoGAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14166">
        <property type="entry name" value="SLIT-ROBO RHO GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="4">
        <location>
            <begin position="28"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="424" mass="49683" checksum="FE6AB0908254FF14" modified="2020-06-17" version="1">MHLICTCATPAFRHPHNPPTALTHTFTDKIRNQLVEQFRCLEQQSESRLQLLQDLQEFFRRKAELQLEYSRGLDKLAERYSAKIRTSREHQHFKKDQNLLSTVNCWYLVLDQTRRESRDHATLSDIYNNNVIVRLAHVGEDVIRLFKKSKDIGVQMHEELVKVTNELYTVMKTYHMYHTESLSAENKLKEAEKQEEKHIGNPMTLVAVLLRYGHDERPQRRSSVKKMEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNALVAKYYIHDVQNSPGTHHENLPVCRIYRCMDIRGDKLKFMDTHSQIFCPPARFDYQPHMGDEVCQVSAQQPVQTELLMRYHQLQSRLATLKIEMKRKTLDATLQTLQDMLTVEDFDVSEAFQHSQSTESVKSASSDSYMSKANIAKRRANQQETEGFYFTVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKE2</accession>
    <name>A0A673AKE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="17">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="17">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115410885</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-acetyl-D-glucosamine + UDP-alpha-D-galactose = beta-D-galactosyl-(1-&gt;4)-N-acetyl-D-glucosamine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17745"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60152"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="2.4.1.90"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:17746"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Golgi apparatus membrane</location>
            <topology evidence="2 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_029978569.1">
        <property type="nucleotide sequence ID" value="XM_030122709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AKE2"/>
    <dbReference type="Ensembl" id="ENSSORT00005031015.1">
        <property type="protein sequence ID" value="ENSSORP00005030170.1"/>
        <property type="gene ID" value="ENSSORG00005014402.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410885"/>
    <dbReference type="InParanoid" id="A0A673AKE2"/>
    <dbReference type="OrthoDB" id="10016069at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003831">
        <property type="term" value="F:beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003945">
        <property type="term" value="F:N-acetyllactosamine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000028">
        <property type="entry name" value="beta-1,4-galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF5">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14 17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 17" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="93"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="231"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="363" mass="41992" checksum="2294FF6E45A1843D" modified="2020-06-17" version="1">MLKKLFKLSAIFALLSVACFVFILFHRKDTTFDILIEKYSKENGNGTLFLEIKGRIQEFLKKEDDRPKNSEKKTEIPTTDVSTAASTVVLGACPDTPPKLVGPLQVDFFTRRTLDEVRKELKGSLQEGGRYKPTECISKHKVAIIIPFRNRYEHLVHWLHYLHPILRRQQLDYGVYVINQDGDGIFNRAKLMNVGFVETQKYDSYECFVFSDIDLVPIDDRNLYRCFDKPRHLAVAMDKFNFQLPYNTYFGGVSSLSKAQYMRINGFPNTYWGWGGEDDDIYKRIVFRGMSISRPDSVIGKYKMVKHKRDLHNEPNPKNPDKLGHTQSTMDQDGINSLKYTVKKVEKDVLYTFITVDVDAPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMM7</accession>
    <name>A0A673AMM7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tmc2a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AMM7"/>
    <dbReference type="FunCoup" id="A0A673AMM7">
        <property type="interactions" value="375"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031364.1">
        <property type="protein sequence ID" value="ENSSORP00005030509.1"/>
        <property type="gene ID" value="ENSSORG00005014550.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AMM7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050910">
        <property type="term" value="P:detection of mechanical stimulus involved in sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060005">
        <property type="term" value="P:vestibular reflex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF17">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="553"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="610"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="433"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="652"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="731"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="745"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="764" mass="86590" checksum="70E2C03C7F94E95B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APU8</accession>
    <name>A0A673APU8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Atonal bHLH transcription factor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">atoh7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673APU8"/>
    <dbReference type="FunCoup" id="A0A673APU8">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032583.1">
        <property type="protein sequence ID" value="ENSSORP00005031700.1"/>
        <property type="gene ID" value="ENSSORG00005015088.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APU8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003407">
        <property type="term" value="P:neural retina development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19714">
        <property type="entry name" value="bHLH_TS_ATOH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000025">
        <property type="entry name" value="protein atonal homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032663">
        <property type="entry name" value="ATOH7_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF162">
        <property type="entry name" value="TRANSCRIPTION FACTOR ATOH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="32"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="140" mass="16352" checksum="BB0E4F7426674D68" modified="2020-06-17" version="1">MKSRRPSCTDSGSESSSEPDSKSPEKFETATRRRMAANARERKRMQGLNTAFDRLRKVVPQWGQDKKLSKYETLQMALSYIMALNRILTDTRRHSAPHRQWLDLQFDCVQPENYAMSEQDYIHSFSYHLGESKYICIALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673APX3</accession>
    <name>A0A673APX3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Taperin</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673APX3"/>
    <dbReference type="Ensembl" id="ENSSORT00005032608.1">
        <property type="protein sequence ID" value="ENSSORP00005031725.1"/>
        <property type="gene ID" value="ENSSORG00005015093.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673APX3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025903">
        <property type="entry name" value="Phostensin/Taperin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025907">
        <property type="entry name" value="Phostensin/Taperin_PP1-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026671">
        <property type="entry name" value="PPP1R18/Tprn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21685:SF1">
        <property type="entry name" value="TAPERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21685">
        <property type="entry name" value="TON-B BOX DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13914">
        <property type="entry name" value="Phostensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13916">
        <property type="entry name" value="Phostensin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phostensin/Taperin N-terminal" evidence="7">
        <location>
            <begin position="15"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Phostensin/Taperin PP1-binding" evidence="6">
        <location>
            <begin position="377"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="17"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="259"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="330"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="510" mass="54631" checksum="EDA8E6CF8D680D39" modified="2020-06-17" version="1">EHASLLFNPSRKTLRDVWRRRRPSAAAGEARPAADREDGGPESLVLQESLGPLQENPFIKLEKERRRRQDRENAARPVQHILELYGSVPGIRTIRADNIIIIESDPDYFPEGGDAKIRAKEVVIYDPALSDGPGRVSRMLQKFDSNYGKLQKKSHSTENLLDLDCSASGRPRLWPKPQPDLVPKPRAGPPSPVPCSPETRRARAESPTKFPRSSPDYEIRPSPTPDLSRIPAGDAQARALANLRLQSRNSFTVIPKRRLAASSAATSPVPPGPVKSSPSHRAVEVPTPGVPTSHPPTATVTPSKRSEEPRQKEPPPPVATSPVCASPSVLSPPPSSPPAPSSPPSSPAVPVSPPASPSSPGPSPPSPAKEPPPPVDQLPVTNIDDIEVEPPQRVPAPSPLVHRKKGNTFTVVPKRRVEPEVQPGSPEPPQDAPSADAPPGSTPPQAPYAQLGSLLKKRYPAAEEIQVIGGYLSLAKSCLSKTGSTGKKVRAKPEHVGGCSFYQSGKRCTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQL5</accession>
    <name>A0A673AQL5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxysteroid dehydrogenase-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hsdl2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AQL5"/>
    <dbReference type="FunCoup" id="A0A673AQL5">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031568.1">
        <property type="protein sequence ID" value="ENSSORP00005030707.1"/>
        <property type="gene ID" value="ENSSORG00005014641.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQL5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09762">
        <property type="entry name" value="HSDL2_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000301">
        <property type="entry name" value="Hydroxysteroid dehydrogenase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051935">
        <property type="entry name" value="HSDL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006133">
        <property type="entry name" value="PRK08278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42808:SF3">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="9">
        <location>
            <begin position="322"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="419" mass="44652" checksum="7956F858E90601A9" modified="2020-06-17" version="1">MLQNTGKLAGCTLFITGASRGIGKAIALKAAKDGANIVIAAKTAEPHPKLPGTIYTAAQEVEAAGGKALACVVDIRDEQQIGDAVQKAVDKFGGIDILVNNASAISLTGTLATPMKRVDLMLGINLRGTYLTSKLVIPHLLKSRSPHILNLSPPLNLNPIWFKNHTAYTMAKYGMSMCVLGMAEEFRGQIAVNALWPKTAIQTAAMDMLGGEGVAKQCRTADIMADAAYAVLTQPTDYTGHFLVDEDVLKGQGVQDFELYAVQPGHPLLPDFFLDEAPETLVQKMEEHGATPAFKPASSSATPPSAGPIESTFDVIRGVINEDVVKSTQGVYKFDLSGKFRGWDFVDVSVSGSGSAGQGEPALKADVIMTMDSGDFSKMFAGKLKPTMAFMSGKLRIKGDMTLAIKLEKLMNRMAKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AS57</accession>
    <name>A0A673AS57_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutaredoxin and cysteine rich domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">grxcr2</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AS57"/>
    <dbReference type="Ensembl" id="ENSSORT00005033274.1">
        <property type="protein sequence ID" value="ENSSORP00005032386.1"/>
        <property type="gene ID" value="ENSSORG00005015361.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AS57"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033023">
        <property type="entry name" value="GRXCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46926">
        <property type="entry name" value="GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46926:SF1">
        <property type="entry name" value="GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="25982" checksum="BE95E33B22429E84" modified="2020-06-17" version="1">MDEFHRKLNQRYEGAKPRKVRFKLASSYSGRVLKHVYEDGQELDSPEEKYPHSFVHRKIPPSHLEMEQFYVYPPTGLIAQRINVYRGMGNYKPAVTQTEETEGDDKSSVLDFGKIIIYTSNLRIIRAPPRKPDTLRHHTVAAVDMEGYPKAKERGSQDFRRTFLQVQHNCLSVDSCQHCGGSGCAPCSLCHGSKLSMLANRFNESISDLRCQACYPYGLEKCQSCSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASK0</accession>
    <name>A0A673ASK0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ASK0"/>
    <dbReference type="Ensembl" id="ENSSORT00005033444.1">
        <property type="protein sequence ID" value="ENSSORP00005032551.1"/>
        <property type="gene ID" value="ENSSORG00005015449.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASK0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040118">
        <property type="entry name" value="C144A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039497">
        <property type="entry name" value="CC144C-like_CC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021885">
        <property type="entry name" value="DUF3496"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22245">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 144A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22245:SF3">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 144A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14915">
        <property type="entry name" value="CCDC144C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12001">
        <property type="entry name" value="DUF3496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC144C-like coiled-coil" evidence="5">
        <location>
            <begin position="106"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3496" evidence="4">
        <location>
            <begin position="315"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="46"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="308"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="447" mass="51805" checksum="9DF67E909253F946" modified="2020-06-17" version="1">MAEDFDELTQSSDTATDDLDSPTAGYRHASLLIQKLDSATLDSRSMVKLQNIFHEYERSIQKLKSRHGYLADKVSQLEMERVELKSSLDEMKDVKSALERNQLELFQLKQEQENRRNATMMYNTTKDKLKRTEEHQQFEVQERQKVELTLRNLELEMRTLVNNMKQVHLETQRLLAQERSARALQENLLSSHLRKQQEIEEESKRNIKTSRRSQLEKANDELKDQLASLKRSNHSSEQLERSKHQLEEELLDLRRRLEAAQAEQNQVDHVRRDAEDRARQEIQQKLEQVNLFLQTQAASQEALDQIKAANEANLRSQLEHKIRELENELGRVRSTQQDSLNQREMTRTELDRYRQLYGEELRLRKSLAAKLESRLSDANSKLLSERSRSLIASSFANSSLGGPSLDRGSLGPLNRSLGLGLSLLNPVSEGQSSRVEDYLAKVCTVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AST1</accession>
    <name>A0A673AST1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">m7GpppN-mRNA hydrolase NUDT17</fullName>
            <ecNumber evidence="8">3.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nucleoside diphosphate-linked moiety X motif 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nudt17</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a decapping enzyme capable of hydrolyzing monomethylated capped RNAs (in vitro). Hydrolyzes monomethylated capped RNA after alpha and beta phosphates to form N(7)-methyl-GDP. Shows low activity towards unmethylated capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.62" evidence="8"/>
    <dbReference type="RefSeq" id="XP_030013720.1">
        <property type="nucleotide sequence ID" value="XM_030157860.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030013721.1">
        <property type="nucleotide sequence ID" value="XM_030157861.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030013723.1">
        <property type="nucleotide sequence ID" value="XM_030157863.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030013724.1">
        <property type="nucleotide sequence ID" value="XM_030157864.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030013725.1">
        <property type="nucleotide sequence ID" value="XM_030157865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AST1"/>
    <dbReference type="FunCoup" id="A0A673AST1">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033539.1">
        <property type="protein sequence ID" value="ENSSORP00005032641.1"/>
        <property type="gene ID" value="ENSSORG00005015495.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435443"/>
    <dbReference type="CTD" id="200035"/>
    <dbReference type="InParanoid" id="A0A673AST1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04694">
        <property type="entry name" value="NUDIX_Nudt17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000033">
        <property type="entry name" value="nucleoside diphosphate-linked moiety X motif 17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033716">
        <property type="entry name" value="Nudt17_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF1">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE-LINKED MOIETY X MOTIF 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="13">
        <location>
            <begin position="85"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="294" mass="32463" checksum="DD2955F1BCB31439" modified="2020-06-17" version="1">MRRILVHVCKDRASPQRASFVQSVTGQFSDGGDDEVEVNCSLEKNQFILYRGDQNQRVPLKRPAFCPIKHLSVTEAAAIPLDVQQRGIDVGVAILLQSANQRVLLTRRAKELRIFPNVWVPPGGHVEPDETLLDAGLRELKEETGLKLDPVAASPKILGLWESVFPPMLSRGLPHRHHIVVYMMLHSSLTHLQIQDSVRPSPAEVSACLWADRHLVRAVVSAVDGEDAEVRVDDLPDTVSVCQVSEDGGLSDVSLPLEVFITRAPSSGPDLERVSTGTKFALELWLKNLEMSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASU5</accession>
    <name>A0A673ASU5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein argonaute-2</fullName>
            <shortName evidence="12">Argonaute2</shortName>
            <ecNumber evidence="12">3.1.26.n2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Eukaryotic translation initiation factor 2C 2</fullName>
            <shortName evidence="12">eIF-2C 2</shortName>
            <shortName evidence="12">eIF2C 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Protein slicer</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AGO2</name>
        <name evidence="15" type="synonym">ago2</name>
        <name evidence="12" type="synonym">EIF2C2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include ago2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by ago2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.n2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of dicer1, ago2 and tarbp2. Note that the trimeric RLC/miRLC is also referred to as RISC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
            <location evidence="1 12">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However, while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.n2" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A673ASU5"/>
    <dbReference type="Ensembl" id="ENSSORT00005033554.1">
        <property type="protein sequence ID" value="ENSSORP00005032656.1"/>
        <property type="gene ID" value="ENSSORG00005015474.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASU5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070551">
        <property type="term" value="F:endoribonuclease activity, cleaving siRNA-paired mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098808">
        <property type="term" value="F:mRNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035278">
        <property type="term" value="P:miRNA-mediated gene silencing by inhibition of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035279">
        <property type="term" value="P:miRNA-mediated gene silencing by mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.2300:FF:000005">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AGO2"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR028602">
        <property type="entry name" value="AGO2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
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    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 12" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8 12" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="13">
        <location>
            <begin position="219"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="14">
        <location>
            <begin position="507"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="587"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="659"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="797"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="849" mass="96629" checksum="C6717990B0C13F97" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUP1</accession>
    <name>A0A673AUP1_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">Leucine-rich repeat-containing protein 14B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">si:ch73-174h16.4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRAME family. LRRC14 subfamily.</text>
    </comment>
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        <property type="interactions" value="68"/>
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        <property type="protein sequence ID" value="ENSSORP00005033370.1"/>
        <property type="gene ID" value="ENSSORG00005015799.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUP1"/>
    <dbReference type="OrthoDB" id="6479713at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Leucine rich repeat containing 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050694">
        <property type="entry name" value="LRRC14/PRAME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224">
        <property type="entry name" value="SIMILAR TO PREFERENTIALLY EXPRESSED ANTIGEN IN MELANOMA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="560" mass="62211" checksum="A38164C94FB26D07" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVI9</accession>
    <name>A0A673AVI9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">H-2 class II histocompatibility antigen, A-U alpha chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115436131</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030014745.1">
        <property type="nucleotide sequence ID" value="XM_030158885.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030014746.1">
        <property type="nucleotide sequence ID" value="XM_030158886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AVI9"/>
    <dbReference type="Ensembl" id="ENSSORT00005033233.1">
        <property type="protein sequence ID" value="ENSSORP00005032346.1"/>
        <property type="gene ID" value="ENSSORG00005015341.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033235.1">
        <property type="protein sequence ID" value="ENSSORP00005032348.1"/>
        <property type="gene ID" value="ENSSORG00005015341.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033237.1">
        <property type="protein sequence ID" value="ENSSORP00005032350.1"/>
        <property type="gene ID" value="ENSSORG00005015341.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436131"/>
    <dbReference type="OrthoDB" id="8935021at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002504">
        <property type="term" value="P:antigen processing and presentation of peptide or polysaccharide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05767">
        <property type="entry name" value="IgC1_MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001003">
        <property type="entry name" value="MHC_II_a_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF105">
        <property type="entry name" value="RLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DP ALPHA-1 CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00993">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0491">MHC II</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627728" evidence="12">
        <location>
            <begin position="19"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="108"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="245" mass="27645" checksum="548B5065B3207D0B" modified="2020-06-17" version="1">MVCFAIIILILNAFGAFSQTPITHEFTFYVGCFFNGTAEVGFQLDNEEIMYVDFQRQEAVVTLPKFIVFDPSTFMAVYHVYRNSLRQKKNCLAVLDYVKVFEQSNIPPEVEDPPDSILYPSEEVELGVENSLICFVNNFFPPSINVIWTKNGDVVSEGVSLSRYYPNNDQTFRQFSTLTFTPKQGDMYSCTVEHPALERPNTRIWEVDLGHPSIGLDIYCGVGLTVALLGVATGTFLMAKAHFVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVW2</accession>
    <name>A0A673AVW2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115431295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030007413.1">
        <property type="nucleotide sequence ID" value="XM_030151553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AVW2"/>
    <dbReference type="FunCoup" id="A0A673AVW2">
        <property type="interactions" value="1244"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034689.1">
        <property type="protein sequence ID" value="ENSSORP00005033776.1"/>
        <property type="gene ID" value="ENSSORG00005015995.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431295"/>
    <dbReference type="InParanoid" id="A0A673AVW2"/>
    <dbReference type="OrthoDB" id="5835702at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000062">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="246" mass="27157" checksum="02ABACA3D9F833B8" modified="2020-06-17" version="1">MSRGSNAGFDRHITIFSPEGRLYQVEYAFKAISQSGLTSVGVRGADSVVVVTQKKVPDKLLDSSTVTNMFKLTPRIGCVMTGHNADSRSQVHRARVEAGEWKYKFGYDINADVLCRRLADISQVYTQNAEMRPLGCCMILVAIDPQQGPVLYKCDPAGYYCGFRATAVGAKQTEANGYLEKKLKKKPDLSHERTVQLAISCLSSVLSLDFKASELEVGVVTKDKPKFRTLSEAEIETHLVAIAERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B060</accession>
    <name>A0A673B060_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B060"/>
    <dbReference type="Ensembl" id="ENSSORT00005035698.1">
        <property type="protein sequence ID" value="ENSSORP00005034774.1"/>
        <property type="gene ID" value="ENSSORG00005016393.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B060"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="73" mass="7868" checksum="2EFC5A0CCE0066A8" modified="2020-06-17" version="1">MDDFSTIITGCHDGQICLWDMTPELEGEGWGGSSAVLSVFSHSIGWMICSVGRSFTQVSPSTSLRLLLSLQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0N3</accession>
    <name>A0A673B0N3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxynucleoside triphosphate triphosphohydrolase SAMHD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">samhd1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:46149"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>dATP + H2O = 2'-deoxyadenosine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17256"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61404"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:67649"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>dCTP + H2O = 2'-deoxycytidine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80083"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:80084"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>dGTP + H2O = 2'-deoxyguanosine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:61429"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:15194"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>dTTP + H2O = thymidine + triphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80079"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:80080"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAMHD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029995215.1">
        <property type="nucleotide sequence ID" value="XM_030139355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B0N3"/>
    <dbReference type="FunCoup" id="A0A673B0N3">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036691.1">
        <property type="protein sequence ID" value="ENSSORP00005035736.1"/>
        <property type="gene ID" value="ENSSORG00005014922.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422806"/>
    <dbReference type="InParanoid" id="A0A673B0N3"/>
    <dbReference type="OrthoDB" id="9991235at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045088">
        <property type="term" value="P:regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000015">
        <property type="entry name" value="Deoxynucleoside triphosphate triphosphohydrolase SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2760:FF:000002">
        <property type="entry name" value="SAM and HD domain-containing deoxynucleoside triphosphate triphosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="15" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SAM" evidence="25">
        <location>
            <begin position="39"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="26">
        <location>
            <begin position="156"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="623" mass="71586" checksum="7694ACBA358B0901" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2J6</accession>
    <name>A0A673B2J6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Growth arrest and DNA damage-inducible protein GADD45 alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gadd45ab</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GADD45 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B2J6"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037372.1">
        <property type="protein sequence ID" value="ENSSORP00005036411.1"/>
        <property type="gene ID" value="ENSSORG00005017162.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2J6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000012">
        <property type="entry name" value="growth arrest and DNA damage-inducible protein GADD45 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024824">
        <property type="entry name" value="GADD45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411:SF3">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="9">
        <location>
            <begin position="66"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="198" mass="21820" checksum="677E6AE80B1468EC" modified="2020-06-17" version="1">MSVELRGVYYCSGAWSGHSEHRLSAREQSGSALDITGQQTDRMCNMTFEENSGDNSSERMESVAKALEEVLSSALPQGCITVGVYEAAKSLNADPDNVVLCLLATDDEEDVALQIHFTLIQAFCCENDINILRVSNMRRLAEILGGVKPGGDLDLHCVLVTNPQSSLWKDPALSKVNRFCRDSRCLDQWVPVINLPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3P5</accession>
    <name>A0A673B3P5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B3P5"/>
    <dbReference type="Ensembl" id="ENSSORT00005036930.1">
        <property type="protein sequence ID" value="ENSSORP00005035974.1"/>
        <property type="gene ID" value="ENSSORG00005016978.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3P5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000145">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF8">
        <property type="entry name" value="SPEG NEIGHBOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="39"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="154" mass="17575" checksum="1A8AF5CEB8CA4483" modified="2020-06-17" version="1">MEISSPITSDEEYLSPLEESMDYGPEPRSIIDTRFKQPPAFLVTMTDQAVIEGQEVTMFVRISGQPKPMLYWLRDRVTVKTGPRHIVGETEEGTFEMKIKSAVRSDSGIYICKIINEYGTKQCEARLEVKGMCVFPPTFHYFPKRPLMSVLSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B8E2</accession>
    <name>A0A673B8E2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RabBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">myripb</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B8E2"/>
    <dbReference type="FunCoup" id="A0A673B8E2">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039604.1">
        <property type="protein sequence ID" value="ENSSORP00005038611.1"/>
        <property type="gene ID" value="ENSSORG00005018040.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B8E2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000018">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051745">
        <property type="entry name" value="Intracell_Transport_Effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006788">
        <property type="entry name" value="Myrip/Melanophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTIC BASIC PROTEIN MOBP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555:SF6">
        <property type="entry name" value="RAB EFFECTOR MYRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04698">
        <property type="entry name" value="Rab_eff_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RabBD" evidence="8">
        <location>
            <begin position="4"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="263"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="428"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="598"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="428"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="480"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="520"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="631"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="672"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="732"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="812"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="833" mass="93788" checksum="7C08F59A79521958" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9Y3</accession>
    <name>A0A673B9Y3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">spns2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B9Y3"/>
    <dbReference type="FunCoup" id="A0A673B9Y3">
        <property type="interactions" value="366"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038252.1">
        <property type="protein sequence ID" value="ENSSORP00005037278.1"/>
        <property type="gene ID" value="ENSSORG00005017244.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B9Y3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046624">
        <property type="term" value="F:sphingolipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003376">
        <property type="term" value="P:sphingosine-1-phosphate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
        <property type="entry name" value="MFS_spinster_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF4">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE TRANSPORTER SPNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="456"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="58"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="524" mass="56991" checksum="68FA2F0EC5F14110" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAI2</accession>
    <name>A0A673BAI2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane 4 L six family member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115438372</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the L6 tetraspanin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BAI2"/>
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    </dbReference>
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        <property type="gene ID" value="ENSSORG00005018380.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198:SF4">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="190" mass="20332" checksum="9AA6717CCD8C0772" modified="2020-06-17" version="1">MCCTGKCARLVGLILLPSAFVCMISNLLLFFPNGVTGLDTDHVSLQVWLMGGLIGGGLFMLCPSCSAIRMLNSVFSSAFGVIGAIYCTSVASAALAIGPKCRLSDGKWEYPFENLGTGNSSYLVNQDLWTECAYPENVVMWHVVLFSILLSMGLLQLVLCGIQVINGCIGCICGDCRDSKEVSVKTQNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAT7</accession>
    <name>A0A673BAT7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BAT7"/>
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        <property type="protein sequence ID" value="ENSSORP00005037573.1"/>
        <property type="gene ID" value="ENSSORG00005017633.1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="35"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="130"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="244"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="357" mass="41883" checksum="D9FD5463B2B04211" modified="2020-06-17" version="1">MCLNLQIIVIVRRTHPSDVNLSTVWSISTCLLHQYHYVHELKTWTEAQSYCRRHYTDLATIENSFKMDQLINAVSGESVQSWIGLFRNPNFKWSDGSSFSFTYWYDDYNPLGSSKIICVWSISTCLLHQYHYVHELKTWTEAQSYCRRHYTDLATIENSFEMDQLINAVSDKSVSEVWIGLFSEVDWRWSDGYTGNESRYRHWDPDWSEPDFKGATELCVLYRSYGWWDIRCDGHFPFICSSEFVYINQSLNWTAAQSYCRSNYVDLATVKNPSDEQKIQNVTPSGQLAWIGLFRNPNFNWSDGSSFSFTYWYGGSNLLGTSKIICGVTSLEHHHSGEWRFFPCDHRRPFVCHSIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BC78</accession>
    <name>A0A673BC78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HAUS augmin-like complex, subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">haus5</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029987940.1">
        <property type="nucleotide sequence ID" value="XM_030132080.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029987941.1">
        <property type="nucleotide sequence ID" value="XM_030132081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BC78"/>
    <dbReference type="Ensembl" id="ENSSORT00005039023.1">
        <property type="protein sequence ID" value="ENSSORP00005038033.1"/>
        <property type="gene ID" value="ENSSORG00005017834.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417886"/>
    <dbReference type="CTD" id="23354"/>
    <dbReference type="InParanoid" id="A0A673BC78"/>
    <dbReference type="OrthoDB" id="2019614at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029131">
        <property type="entry name" value="HAUS5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28588">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28588:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14817">
        <property type="entry name" value="HAUS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="335"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="659" mass="75878" checksum="65740893F8DCBCCB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BC86</accession>
    <name>A0A673BC86_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fizzy-related protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cdh1/Hct1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115417894</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987961.1">
        <property type="nucleotide sequence ID" value="XM_030132101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BC86"/>
    <dbReference type="Ensembl" id="ENSSORT00005039234.1">
        <property type="protein sequence ID" value="ENSSORP00005038242.1"/>
        <property type="gene ID" value="ENSSORG00005017835.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417894"/>
    <dbReference type="InParanoid" id="A0A673BC86"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000025">
        <property type="entry name" value="FIZZY-related 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="12">
        <location>
            <begin position="181"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="311"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="441"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="495" mass="54995" checksum="7A679F94EE09C101" modified="2020-06-17" version="1">MDSDYEHRLLRQINIQNDNLSPVKMTQTLHSQSPNGYLLSSSGKMGDRFIPTRAGANWNINFHRINENKKSPNQNRKTKDATSDNGKADGLAYSALLKNELLGAGIEKILDPQTEDRRLQPSTPEKESLFSYSLHTKRSSPDNGTNISPYSLSPVSNKSQKLLRSPRKPSRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGYIQIWDAGAGKKLFALEGHTARVGALAWNADQLSSGSRDRLILQRDVRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNYSSLSPVQTYMDHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTSQPLQCMDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPALTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKESVSVLNLFTRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BDG4</accession>
    <name>A0A673BDG4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BDG4"/>
    <dbReference type="Ensembl" id="ENSSORT00005040844.1">
        <property type="protein sequence ID" value="ENSSORP00005039810.1"/>
        <property type="gene ID" value="ENSSORG00005018591.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BDG4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10574">
        <property type="entry name" value="EVH1_SPRED-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000052">
        <property type="entry name" value="Sprouty-related, EVH1 domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041937">
        <property type="entry name" value="SPRE_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF19">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="148" mass="16259" checksum="0457E0E9701649DF" modified="2020-06-17" version="1">AQGDVEGSPVVMTRDDSSGGWVPLGGGGLSHVVICKGRSHEGRGRREYIIRGERLRDRAPVLECAVQRGLVYNKVNPIFHHWRVEDRKFGLTFQSPADAISFEKGLQTVIDKLDRGSDSPSSSTPEEADTEDDGQAVSIRTLCGMFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BDR5</accession>
    <name>A0A673BDR5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosomal protein S6 kinase-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps6kl1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BDR5"/>
    <dbReference type="FunCoup" id="A0A673BDR5">
        <property type="interactions" value="433"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041724.1">
        <property type="protein sequence ID" value="ENSSORP00005040671.1"/>
        <property type="gene ID" value="ENSSORG00005018994.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BDR5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02677">
        <property type="entry name" value="MIT_SNX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051866">
        <property type="entry name" value="Intracell_Sig-Traffick_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508:SF4">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="110"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="532" mass="59035" checksum="1FB444BE8D30946A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEE0</accession>
    <name>A0A673BEE0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tumor necrosis factor alpha-induced protein 8-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tnfaip8l1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TNFAIP8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987989.1">
        <property type="nucleotide sequence ID" value="XM_030132129.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029987990.1">
        <property type="nucleotide sequence ID" value="XM_030132130.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029987991.1">
        <property type="nucleotide sequence ID" value="XM_030132131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BEE0"/>
    <dbReference type="FunCoup" id="A0A673BEE0">
        <property type="interactions" value="678"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039748.1">
        <property type="protein sequence ID" value="ENSSORP00005038753.1"/>
        <property type="gene ID" value="ENSSORG00005018107.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417909"/>
    <dbReference type="CTD" id="126282"/>
    <dbReference type="InParanoid" id="A0A673BEE0"/>
    <dbReference type="OrthoDB" id="10055976at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.160:FF:000001">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 8-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.160">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008477">
        <property type="entry name" value="TNFAIP8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038355">
        <property type="entry name" value="TNFAIP8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12757:SF2">
        <property type="entry name" value="TUMOR NECROSIS FACTOR ALPHA-INDUCED PROTEIN 8-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12757">
        <property type="entry name" value="TUMOR NECROSIS FACTOR INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05527">
        <property type="entry name" value="TNFAIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="186" mass="21627" checksum="98389C2BE027EE8D" modified="2020-06-17" version="1">MDTFSTKSLALQAQKKLMSKMASKSMANLFIDDTSSEVLDELYRVTKEYTRNRKESQKIIKNLIKMVVKLGVLYRNNQFNTEELILVENFRKKVHTLAMTAVSFHQIEFTFDRRVMSAILNECRDLLHQAIKRHLTAKSHSRVNHVFNHFADCDFLVALYGPSEVYRAHLQRICNGVNQMLDEGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFZ2</accession>
    <name>A0A673BFZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kynurenine 3-monooxygenase</fullName>
            <ecNumber evidence="9">1.14.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Kynurenine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">kmo</name>
        <name evidence="9" type="synonym">KMO</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-kynurenine + NADPH + O2 + H(+) = 3-hydroxy-L-kynurenine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:20545"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion outer membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the aromatic-ring hydroxylase family. KMO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.9" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673BFZ2"/>
    <dbReference type="FunCoup" id="A0A673BFZ2">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040546.1">
        <property type="protein sequence ID" value="ENSSORP00005039537.1"/>
        <property type="gene ID" value="ENSSORG00005018440.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BFZ2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004502">
        <property type="term" value="F:kynurenine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070189">
        <property type="term" value="P:kynurenine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000185">
        <property type="entry name" value="Kynurenine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01971">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027545">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028:SF2">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="2 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="437"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="11">
        <location>
            <begin position="32"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="492" mass="55700" checksum="032E3C258D9CD923" modified="2020-06-17" version="1">MPLQWAVRGSDHQRTLSTMEDSSSKPAKKKVVAVVGGGLVGALNACFFAKRGFDVEVFETREDIRTAKIVKGRSINLALSHRGRSALKHVGMEEKIVSQGIPMHARMIHSLTGKQSPIPYGKKGQVTAETYPNTKLNFDHKLQDWNAETGIMTFVRSDGTKANIEADLIVGCDGAFSAIRKQFLRRSRFNYSQTYIPHGYMELTMPPINGEFAMKPNYLHIWPRNKFMMIALPNLDKTFTCTLFMPFEEFEKITTGDEVITFFQTYFPDAIPLIGVDALKRDYFRLPAQAMVSVKCSPYHIGDKCVLMGDAAHAVVPFYGQGMNAGFEDCIVFDEVMNQFNEDLSKCAVLPEYTRVRVPDDHAIADLAMYNYVEMRAHVNSKWFLFRKHVDNILHLLMPKTIIPLYTMVSVFIWELLPQNIHFKLNGIKSLSFSHDHTFLSSHCIVGIGHVVENVLFFCAGVQRGCGVSVNLDLMCVWQCLLAFLCLFLYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BG95</accession>
    <name>A0A673BG95_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tribbles homolog 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">trib2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984245.1">
        <property type="nucleotide sequence ID" value="XM_030128385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BG95"/>
    <dbReference type="Ensembl" id="ENSSORT00005040659.1">
        <property type="protein sequence ID" value="ENSSORP00005039647.1"/>
        <property type="gene ID" value="ENSSORG00005018493.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415002"/>
    <dbReference type="CTD" id="28951"/>
    <dbReference type="InParanoid" id="A0A673BG95"/>
    <dbReference type="OrthoDB" id="410920at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031434">
        <property type="term" value="F:mitogen-activated protein kinase kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14022">
        <property type="entry name" value="PK_TRB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000153">
        <property type="entry name" value="Tribbles homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000253">
        <property type="entry name" value="tribbles homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF15">
        <property type="entry name" value="TRIBBLES HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="61"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="345" mass="39305" checksum="9DD3A994685DA325" modified="2020-06-17" version="1">MNIQRSNPINISRYGRSRHKAHDFEELSCLRTTESHQSFSPNLGSPSPPETPDSSHCISRIGDYLLLEPLEGDHVFRAAHLHSGEELVCKVFDIGRYQESLAAYFALGQHQHINQILEILLGETRAYVFFERSYGDMHSFVRTCKKLREDEAARLFYQIASAVAHCHDNGLVLRDLKLRKFVFKNEDRNLVKLESLEDTYILDGHDDSLSDKHGCPAYVSPEILNANGSYSGKAADVWSLGVMLYTILVGRYPFHDVEPGSLFSKIRRGHFNIPETLTPKAKCLIRSILRREPAERLTSREILEHPWFTSSGALGGSLVQGRAEREQEQMVPEVNMEEELEQFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BGD3</accession>
    <name>A0A673BGD3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Cholinergic receptor, nicotinic, alpha 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115424919</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BGD3"/>
    <dbReference type="Ensembl" id="ENSSORT00005040699.1">
        <property type="protein sequence ID" value="ENSSORP00005039687.1"/>
        <property type="gene ID" value="ENSSORG00005018516.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BGD3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19016">
        <property type="entry name" value="LGIC_ECD_nAChR_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000008">
        <property type="entry name" value="Cholinergic receptor nicotinic alpha 6 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000051">
        <property type="entry name" value="Cholinergic receptor, nicotinic, alpha 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="20">
        <location>
            <begin position="24"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="21">
        <location>
            <begin position="237"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="355"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="363"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="492" mass="56045" checksum="962DBF7C463A454A" modified="2020-06-17" version="1">LNPDPPCHLYFLFEPSDCFSSKAEDRLFRKLFRRYNQFIRPVENVSDPVTVEFEVSISQLVKVDEVNQIMETNLWLRHIWNDYKLRWTPAEFDGIEFIRVPSNKIWRPDIVLYNNAVGDFLVEDKTKALLKFDGTITWIPPAIFKSSCPMDITYFPFDYQNCSMKFGSWTYDKAKIDLVLIGSKVNLKDFWESGEWEIIDAPGYKHDIKYNCCEEIYPDITYSFYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPMTHTMPGWVRSVFLKVLPRIMLMRRPIDEDGMAGGLDSSGEAGGAGGGGGGGGGKGGKKRKNSLMQVSCHFCVGCTGKKCPCPCQHGKETPDTPDTGKMSRQLSLQSINTVVAFSVVSPEIKQAIESVKYIAENMRSRNKAKEVEDDWKYVAMVIDRIFLWVFPLFGFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BGM8</accession>
    <name>A0A673BGM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">sfxn1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Amino acid transporter importing serine, an essential substrate of the mitochondrial branch of the one-carbon pathway, into mitochondria. Mitochondrial serine is then converted to glycine and formate, which exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors. May also transport other amino acids including alanine and cysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-alanine(in) = L-alanine(out)</text>
            <dbReference type="Rhea" id="RHEA:70719"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteine(in) = L-cysteine(out)</text>
            <dbReference type="Rhea" id="RHEA:29655"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-serine(in) = L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:35031"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the sideroflexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BGM8"/>
    <dbReference type="FunCoup" id="A0A673BGM8">
        <property type="interactions" value="845"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040821.1">
        <property type="protein sequence ID" value="ENSSORP00005039787.1"/>
        <property type="gene ID" value="ENSSORG00005018581.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BGM8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF8">
        <property type="entry name" value="SIDEROFLEXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="312" mass="34567" checksum="C31022FB503D5ADC" modified="2020-06-17" version="1">MAAELSTSININEPRWDQSTFVGRAKHFFTVTDPRNILLSNEQLAHAHKIITDYRKGVVSPGLTEDELWRAKYIFDSAFHPDTGEKMILIGRMSAQVPMNMTITGCMMTFYRTTPAVLFWQWINQSFNAIVNYTNRSGDAPITVSQLGTAYVSATTGAVATALGLNALTKHISPLIGRFVPFAAVAAANCINIPLMRQRELKHGIPVTDENDNRLGESTKAAQQAISQVVVSRILMASPGMAIPPFLMNHLEKKAFLKCLSCFFSLVFATPLCCALFPQKSSMSVSRLEPELQEKIRANHPGVERVYFNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJB2</accession>
    <name>A0A673BJB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD and ring finger domains 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">phrf1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029992038.1">
        <property type="nucleotide sequence ID" value="XM_030136178.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043777.1">
        <property type="protein sequence ID" value="ENSSORP00005042691.1"/>
        <property type="gene ID" value="ENSSORG00005019775.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420715"/>
    <dbReference type="CTD" id="57661"/>
    <dbReference type="InParanoid" id="A0A673BJB2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16635">
        <property type="entry name" value="mRING-HC-C3HC3D_PHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15536">
        <property type="entry name" value="PHD_PHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047157">
        <property type="entry name" value="PHRF1/Atg35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057031">
        <property type="entry name" value="SFR19-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618:SF20">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23030">
        <property type="entry name" value="SCAF11-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="110"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="191"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="245"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="339"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="585"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1024"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1441"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1644"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1776"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="394"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="20">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="31401" checksum="1D9E3D02EB7DE34F" modified="2020-06-17" version="1">MAEQGAVVMQQNLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPIVYVLDLADRLISKAGPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEASPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNFPEQEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRM8</accession>
    <name>A0A673BRM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BRM8"/>
    <dbReference type="Ensembl" id="ENSSORT00005044958.1">
        <property type="protein sequence ID" value="ENSSORP00005043844.1"/>
        <property type="gene ID" value="ENSSORG00005020229.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRM8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06753">
        <property type="entry name" value="PDZ_PDLIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000019">
        <property type="entry name" value="LIM domain binding 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF9">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="3"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="135" mass="14648" checksum="E621F69FF296CE35" modified="2020-06-17" version="1">MSAYTVSLPGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQGNLIQGDVIVAIDGVSTEGMTHLEAQNKIKMANYNLALTMSKSKRPVPMSPPRMDAALPMVPHQQVCHVNLEGQPFYSKKDKPLCKKHAHAINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSV7</accession>
    <name>A0A673BSV7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673BSV7"/>
    <dbReference type="Ensembl" id="ENSSORT00005044779.1">
        <property type="protein sequence ID" value="ENSSORP00005043672.1"/>
        <property type="gene ID" value="ENSSORG00005020162.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BSV7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06613">
        <property type="entry name" value="STKc_MAP4K3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000031">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF15">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="377" mass="45303" checksum="72E67796792C2449" modified="2020-06-17" version="1">MDFHQRAALDISTRNPQDDFEILLRVGGGTYGEVYKARNKQNGELAAIKVIKMEPEDDFSIIQQEIIIVKSCKHPNIVAYYGSYIRANKLWICMEFCGGGSLQDIYHVTGPLSEPQIAYICREMLQGLDYLHAQKKIHRDIKGANILLNNQGEVKLADFGISAQITATLARRMSFIGTPYWMAPEVAAVEIKGGYNELCDIWSVGITAIELAELQPPMFDVHPLRVLFLMSKSGYQPPKLKDKSKWSSTFYNFVKAMLVRNPKKRPSASKMLSHMFVTQQGLNQELTLDLLEKFRHPEKLKTCLVMDDEEMEVKPIRYRYRYRHRYRYRYRIRIRYRYRYRHRYRYRYRIRIRYRYRYRYRYRIRIRYRYRYRYRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BT72</accession>
    <name>A0A673BT72_9TELE</name>
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        <submittedName>
            <fullName evidence="2">BAALC binder of MAP3K1 and KLF4 b</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BT72"/>
    <dbReference type="Ensembl" id="ENSSORT00005044894.1">
        <property type="protein sequence ID" value="ENSSORP00005043782.1"/>
        <property type="gene ID" value="ENSSORG00005020205.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BT72"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009728">
        <property type="entry name" value="BAALC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14731:SF1">
        <property type="entry name" value="BAALC BINDER OF MAP3K1 AND KLF4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14731">
        <property type="entry name" value="BRAIN AND ACUTE LEUKEMIA CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06989">
        <property type="entry name" value="BAALC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="63" mass="6825" checksum="82D2CEBA2D023329" modified="2020-06-17" version="1">MGCGGSRTDALEPRYLESWTKETESTWLTSTDADIPLSSIQSIPSENSEAGFSSEKTISPGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVI4</accession>
    <name>A0A673BVI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115421185</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A673BVI4"/>
    <dbReference type="Ensembl" id="ENSSORT00005045696.1">
        <property type="protein sequence ID" value="ENSSORP00005044572.1"/>
        <property type="gene ID" value="ENSSORG00005018623.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVI4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SRSF protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000163">
        <property type="entry name" value="SRSF protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000275">
        <property type="entry name" value="SRSF protein kinase 2 isoform X3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF6">
        <property type="entry name" value="SRSF PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="68"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="226"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="450"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="255"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="309"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="351"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="384"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="709" mass="79634" checksum="DF7179044D1D5942" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVT2</accession>
    <name>A0A673BVT2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Endonuclease domain-containing 1 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115420390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BVT2"/>
    <dbReference type="Ensembl" id="ENSSORT00005046375.1">
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        <property type="gene ID" value="ENSSORG00005020776.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVT2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039015">
        <property type="entry name" value="ENDOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN ENDOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472:SF19">
        <property type="entry name" value="ZGC:172339"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025409785" evidence="1">
        <location>
            <begin position="19"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="3">
        <location>
            <begin position="58"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="2">
        <location>
            <begin position="59"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="297" mass="34359" checksum="BF100B043F8DADE9" modified="2020-06-17" version="1">HFCVFLLMVFSIFCLICSFSAEVQKNLSPECKQFLYMGTLPRGLEEQPLKMICQFYEGKPRFVTLYDTVSHIPVYSAYTFKRSDGSKKADAPWMYEPQLSTVTGPREMQQFPSEDVHKSFEDAQAVLDDYSDTVIFERGQLNPDEHQAHPNDKAATYTLTNVVPQVRDFNIGPWKSHEHTIRRRLNNYCRGTAYVVTGVTTSGQMIRRRNINRLAIPTYLWSAYCCIDFDHNAPFSERSKFPAFAAHGLNDRENNMVQEMTIQQLEDFLKRVTYVGNSFQIFYDNCVEVKFATKTHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVY0</accession>
    <name>A0A673BVY0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BVY0"/>
    <dbReference type="Ensembl" id="ENSSORT00005048219.1">
        <property type="protein sequence ID" value="ENSSORP00005047046.1"/>
        <property type="gene ID" value="ENSSORG00005021536.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVY0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21523">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21523:SF14">
        <property type="entry name" value="EXPORTED REPETITIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="221" mass="22282" checksum="EBBC69FE8E94CBDA" modified="2020-06-17" version="1">MCEWECGGGGAFGCCVCDPSVCDPSVCDPSTVCDPSVCDPSTVCDPSVCDPSAVCDPSVCDPSVCDPSTVCDPSVCDPSVCDPSACEPSTVFCDPSVCDPSTVCDPSVCDLSRVCDPSTVCDPSVCDPSTVCDPSVCDPSVCDPSTVCDPSAVCDPSVCDPSTVCDPSVCDPSTVCDPSTVCDPSVFCDPSTVCDPSTCDPSVCDPSTVCDPSVCDPSTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BWU5</accession>
    <name>A0A673BWU5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115432569</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BWU5"/>
    <dbReference type="Ensembl" id="ENSSORT00005047424.1">
        <property type="protein sequence ID" value="ENSSORP00005046265.1"/>
        <property type="gene ID" value="ENSSORG00005021189.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BWU5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="36"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="310" mass="35790" checksum="5F6A31C8A649E854" modified="2020-06-17" version="1">MEKQMDNRTALTFKMTAYAPLYFLLFLIIYIIIIAESSLLIGVVYHEKSLHEPMYLLLCNLAVNGLFGGTALLPAVLSNVLSDSHEISLSLCQIQIYAIHTYAITEFTILAVMSYDRYVAICYPLSYHVIMSQRLVKLIVCMWLYPMLAFLIVLCFTLQLKFCKKAIDKIYCLNYLLVQLSCTKSPITNIIGLFSVAIYTLPQLFMVFYSYAHILKICLLSFNNSKRKALQTCIPHLLSIINYSIGCFFEIAQGRFNITYISYHTKLFLSLYFLICPPMFNPLIYGLSIQVIRVRLLKLFRRQSRQVQVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1P8</accession>
    <name>A0A673C1P8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transmembrane protein 230</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">tmem230b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in trafficking and recycling of synaptic vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TMEM134/TMEM230 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C1P8"/>
    <dbReference type="FunCoup" id="A0A673C1P8">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049115.1">
        <property type="protein sequence ID" value="ENSSORP00005047930.1"/>
        <property type="gene ID" value="ENSSORG00005021882.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1P8"/>
    <dbReference type="OrthoDB" id="5597044at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048489">
        <property type="term" value="P:synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044234">
        <property type="entry name" value="TMEM230"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008590">
        <property type="entry name" value="TMEM_230/134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664">
        <property type="entry name" value="C20ORF30 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05915">
        <property type="entry name" value="TMEM_230_134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00007743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="119" mass="13125" checksum="6385F22B276D3414" modified="2020-06-17" version="1">MPARNVVSNGLPNSKVRYSRLASDDDGYIDLQFKRSPPKVPYKAIALATVLFLIGSLLIIIGSLLLAGYFGVTHSDRTVPVLIIGILVFLPGIYHLRIAYYASKGYPGYSYDDIPDFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C8L3</accession>
    <name>A0A673C8L3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C8L3"/>
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        <property type="protein sequence ID" value="ENSSORP00005051926.1"/>
        <property type="gene ID" value="ENSSORG00005023442.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="59" mass="7139" checksum="F2CDAAADDDCD644A" modified="2020-06-17" version="1">MCKCIFYCIHSFWKDKQPDSNQGHSEEDCAEIYVSDPPLRWNDIPCLQKNYWICERLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CA69</accession>
    <name>A0A673CA69_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CA69"/>
    <dbReference type="Ensembl" id="ENSSORT00005053708.1">
        <property type="protein sequence ID" value="ENSSORP00005052461.1"/>
        <property type="gene ID" value="ENSSORG00005023636.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CA69"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="191"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="304" mass="34788" checksum="936A5BC36053A97D" modified="2020-06-17" version="1">KSDSALSVLTVSPSWLSPGASVTLKCHVKPPSAGWRFYWYKAVPQLSDEPYRYELLPDSSEGTEQEVFTIHGQKNTTGYMCRAGRGDPQFYSKYSLPKFTWYMDSSVSLRVSPNRSQHFTYESISLSCETNSAEWRVRQFYEDFYLFCDDYRWKAQTSPCIISRTVYNGAVFWCESGSGEFSNAVNISTHDDIILESPVHPVTEGQPLTLSCRLWTDGILSDVSFYKNGKLVQTGTSEQLNISAVSKSDEGFYQCERKEVLTRRVQVWMSPESWVSVKCKFNLTFFPVFLQFSKVKSEIKQQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBG9</accession>
    <name>A0A673CBG9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ADAMTS-like 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">adamtsl7</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBG9"/>
    <dbReference type="Ensembl" id="ENSSORT00005050679.1">
        <property type="protein sequence ID" value="ENSSORP00005049478.1"/>
        <property type="gene ID" value="ENSSORG00005022461.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBG9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
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        <property type="entry name" value="TSP1_rpt"/>
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        <property type="entry name" value="TSP1_rpt_sf"/>
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    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF316">
        <property type="entry name" value="LONELY HEART, ISOFORM A"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF05986">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="TSP1"/>
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    </dbReference>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PLAC" evidence="5">
        <location>
            <begin position="487"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="548" mass="61487" checksum="94862BFC3CC9D7AC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBM3</accession>
    <name>A0A673CBM3_9TELE</name>
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        <submittedName>
            <fullName evidence="13">Forkhead box P1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">foxp1b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBM3"/>
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        <property type="interactions" value="675"/>
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        <property type="protein sequence ID" value="ENSSORP00005049533.1"/>
        <property type="gene ID" value="ENSSORG00005022231.1"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000005">
        <property type="entry name" value="Forkhead box P1, isoform CRA_f"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000010">
        <property type="entry name" value="Forkhead box P2 isoform B"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
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        <property type="entry name" value="FOXP"/>
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        <property type="entry name" value="FOXP-CC"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45796">
        <property type="entry name" value="FORKHEAD BOX P, ISOFORM C"/>
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    </dbReference>
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        <property type="entry name" value="FORKHEAD BOX PROTEIN P1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF16159">
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    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
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    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FORK_HEAD_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Fork-head" evidence="12">
        <location>
            <begin position="372"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="10">
        <location>
            <begin position="372"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="156"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="515"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="584" mass="64821" checksum="C69F6D5398BE3041" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CDM0</accession>
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        <recommendedName>
            <fullName evidence="3">PHD finger protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">phf10</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAYP family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030131222.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051407.1">
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        <property type="gene ID" value="ENSSORG00005022775.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417215"/>
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    <dbReference type="InParanoid" id="A0A673CDM0"/>
    <dbReference type="OrthoDB" id="1903104at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071564">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
    <dbReference type="CDD" id="cd15529">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21085">
        <property type="entry name" value="WH_NTD_PHF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000202">
        <property type="entry name" value="PHD finger protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
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    </dbReference>
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        <property type="entry name" value="PHF10_PHD_finger_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888">
        <property type="entry name" value="HL01030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888:SF4">
        <property type="entry name" value="PHD FINGER PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="364"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="420"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="280"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="485" mass="54899" checksum="94D1CD3BC1CD8F4B" modified="2020-06-17" version="1">MAAVLPVRPPCDSNPATPGAQSIKEDIIEEESNDGSQPPKRRRMGSGDSSRSCDTSSQELGPTYFPAENLTEYKWPPDDTGEYYMLQEQVSEYLGVTSFKRKYPDMERRDLSHKEKLYLREQNVITETQCTLGLTALRSDEVIDLMIKEYPTKHSEYSVILQERERQRIAKEYSQMQQQNPQKVEASKVPEYIKKAAKKAAEFNSNFNRERMEERRAYFDLQTHIIQVPQGRFKVLAPELTRTGPYPVALIPGQFQDYYKRYSPNELRYLPLNTALFEPPLDPELPALDSEPDSDDAEDGKDEKRNKNSSDSSSGNTSDVESQEGGGGQGHKSKGKDRTSTQGKDGSHKSHKTTPGYKPKVIPHAICGICEKGKEANKKGKPEALIHCSQCDNSGHPSCLEMSEELVCVIQTYRWQCMECKTCTVCQQPHHEDEMMFCDKCDRGYHTFCVGMDSIPTGLWVCEVCGKDFNTPKKKGAKTPKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CE65</accession>
    <name>A0A673CE65_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CE65"/>
    <dbReference type="Ensembl" id="ENSSORT00005053632.1">
        <property type="protein sequence ID" value="ENSSORP00005052385.1"/>
        <property type="gene ID" value="ENSSORG00005023612.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CE65"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF62">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="11"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="348" mass="38825" checksum="AFD3641C1DA368F8" modified="2020-06-17" version="1">MLANMINSTSPNIRRIVLLGKTGDGKSSLINTIFGENVFKVNHSANSETISCQSATREVSGCKVHMTDTPGFFDTDPYDENVEPVLKCMIECAPGPHAFLIVLKVGKYTEQEKAIISVILKYFSAEALKFATVVFTHGDQLEENKKIGDFVSENPDLRKLVEKCGGRCHVFDNKYWNNQQQDEYRTNRFQIRELLKTIDKTVEENKGRYYTNEMLQSVEEDIQQEKERIRLLPGNKSPEEIREQAKHNVLKKYLPRVVGTVTGILLGALFGSLGTIIAMPGVLGCVPVAVCIGITAAIGGAEVGYEGYEAGAQADTAKEAVKLTWKKAMAYLCEITDILKSTRRKFHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGA7</accession>
    <name>A0A673CGA7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">gyg1b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Glycogenin participates in the glycogen biosynthetic process along with glycogen synthase and glycogen branching enzyme. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23360"/>
            <dbReference type="Rhea" id="RHEA-COMP:14604"/>
            <dbReference type="Rhea" id="RHEA-COMP:14605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140573"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:23361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56560"/>
            <dbReference type="Rhea" id="RHEA-COMP:14606"/>
            <dbReference type="Rhea" id="RHEA-COMP:14607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140574"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:56561"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A673CGA7"/>
    <dbReference type="FunCoup" id="A0A673CGA7">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055887.1">
        <property type="protein sequence ID" value="ENSSORP00005054611.1"/>
        <property type="gene ID" value="ENSSORG00005024450.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGA7"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000025">
        <property type="entry name" value="Glycogenin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="335" mass="37704" checksum="A36A35CB0F8B0070" modified="2020-06-17" version="1">MADQAFVTLATNDNYARGAMVLGKSLRNHNTSKKLVALIGPQVSEPCRNVLKRIFDEVRVVDVLDSGDTAHLAMMKRPDLGVTFTKLHCWTLTQYSKCVFMDADTLVLSNIDELFDRAELSAAPDPGWPDCFNSGVFVFCPSMETYGRLLEYGTKHGSFDGGDQGVLNGFFSDWATADISKHLPFIYNLSSIAIYTYLPAFKQYGGNAKVVHFLGKTKPWNYTFDPKTKRISGSVQEATTHPTFLLDWWTLYSSAVVPMLYEQYGDQPFNSGCVELPYSDMGTFESGQDFSSHAQALEPQMSSEERKQKWEQGQADYLGRDSFDNIQRKLDIFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGR5</accession>
    <name>A0A673CGR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myelin and lymphocyte protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115433725</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030011049.1">
        <property type="nucleotide sequence ID" value="XM_030155189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CGR5"/>
    <dbReference type="Ensembl" id="ENSSORT00005053731.1">
        <property type="protein sequence ID" value="ENSSORP00005052484.1"/>
        <property type="gene ID" value="ENSSORG00005023649.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433725"/>
    <dbReference type="InParanoid" id="A0A673CGR5"/>
    <dbReference type="OrthoDB" id="9940869at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013295">
        <property type="entry name" value="MAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF12">
        <property type="entry name" value="MYELIN AND LYMPHOCYTE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01884">
        <property type="entry name" value="MALPROTEIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="10">
        <location>
            <begin position="17"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="155" mass="17201" checksum="FAFBB7FB6956132E" modified="2020-06-17" version="1">MEYATTSGDVMPTGGKIFITVPDLFFIPEFVFGGLVWILVASTHVNPPNPLGWVMFVSVFCFVFTTLWFFIFLCGGNQNSVWPTLDVVYHFVAVVFFLSASVGLASVTIGNGRLALAPDSLKIYRLDIAAVVMSYVATLLYFIHAIFSAIRWKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CIC3</accession>
    <name>A0A673CIC3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">G protein-coupled receptor 186</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CIC3"/>
    <dbReference type="Ensembl" id="ENSSORT00005054318.1">
        <property type="protein sequence ID" value="ENSSORP00005053064.1"/>
        <property type="gene ID" value="ENSSORG00005023894.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CIC3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000723">
        <property type="entry name" value="GPR_3/6/12_orphan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00644">
        <property type="entry name" value="GPRORPHANR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="47"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="324" mass="35060" checksum="69BDCB2BFABF2159" modified="2020-06-17" version="1">KNDPLKMGLLYLCVILDQLEGTSPYQLPPLTVWGVALCVSGTLIATENAIVVTTILATPSLRAPVFLLLASLGLADLLAGVALILHFLFLFCVEPSDWSELLTSGLLVTSLTASLCSLMGVALDRYLSLSHALTYGSSQSRHRAAILLLLVWVGACVIGAVPAMGWHCLGEPDSCSVARPLTRTYLSLLCGGFLVVVMVTLQLYAGICQVARRHAHAIATQRHFLPANQSYANKHVNGRGFSRLILVLSVFVGCWMPFSLWGLLGDASSPPLYTYATLVPAAGSSLLNPILYSLRNKDIRKVLLQACCPHRYTHNTHIHYPVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CJY1</accession>
    <name>A0A673CJY1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CJY1"/>
    <dbReference type="Ensembl" id="ENSSORT00005053629.1">
        <property type="protein sequence ID" value="ENSSORP00005052383.1"/>
        <property type="gene ID" value="ENSSORG00005023610.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CJY1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF62">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="319" mass="35470" checksum="85BA5EB490FBCAB1" modified="2020-06-17" version="1">MEGSDLLSYSLINTILGEKVFKVNHSPNSGTKTCQSKTTKVNESKVHMIDTPGFFDTYPCDENTEPAMKCMIECAPGPHAFLIVLKVGKYTEHEKAIISVILKYFSAEALKFATVVFTHGDQLEEEKKIGDFVSENPDLSELVKKCSGRCHVFDNKYWNNQQQDEYRTNRFQIRELLKTIDKTVEENKGGYYTNEMLQSVEGDIQQEKERIRLLPGNKSPEEIRKQAKDNVFDKIHPKVAGTVTGILLGAFLGALTLLEPAELLSGGSLALPTGITAAGIIRVIVEGYKGFKAGVSACLRRFFFATVTKCLLLEDSDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CLL4</accession>
    <name>A0A673CLL4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Cholinergic receptor, nicotinic, delta (muscle)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">chrnd</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979378.1">
        <property type="nucleotide sequence ID" value="XM_030123518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CLL4"/>
    <dbReference type="FunCoup" id="A0A673CLL4">
        <property type="interactions" value="284"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054167.1">
        <property type="protein sequence ID" value="ENSSORP00005052913.1"/>
        <property type="gene ID" value="ENSSORG00005023741.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411406"/>
    <dbReference type="CTD" id="1144"/>
    <dbReference type="InParanoid" id="A0A673CLL4"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000010">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000012">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 19" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025704324" description="Cholinergic receptor, nicotinic, delta (muscle)" evidence="19">
        <location>
            <begin position="24"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="20">
        <location>
            <begin position="27"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="21">
        <location>
            <begin position="255"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="60713" checksum="6DFE1FBD03CFCAEF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CM72</accession>
    <name>A0A673CM72_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Endonuclease 8-like 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">DNA glycosylase/AP lyase Neil1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">DNA-(apurinic or apyrimidinic site) lyase Neil1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Endonuclease VIII-like 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nei homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Nei-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">neil1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as a DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029993373.1">
        <property type="nucleotide sequence ID" value="XM_030137513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CM72"/>
    <dbReference type="FunCoup" id="A0A673CM72">
        <property type="interactions" value="160"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058046.1">
        <property type="protein sequence ID" value="ENSSORP00005056746.1"/>
        <property type="gene ID" value="ENSSORG00005025225.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421574"/>
    <dbReference type="CTD" id="79661"/>
    <dbReference type="InParanoid" id="A0A673CM72"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:DNA N-glycosylase activity"/>
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        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="entry name" value="endonuclease 8-like 1 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="endonuclease 8-like 1 isoform X1"/>
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        <property type="entry name" value="MutM-like, N-terminal"/>
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        <property type="entry name" value="Endonuclease-VIII_DNA-bd"/>
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        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
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        <property type="entry name" value="ENDONUCLEASE 8-LIKE 1"/>
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        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
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    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09292">
        <property type="entry name" value="Neil1-DNA_bind"/>
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        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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    </dbReference>
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        <property type="entry name" value="N-terminal domain of MutM-like DNA repair proteins"/>
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        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FPG_CAT"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formamidopyrimidine-DNA glycosylase catalytic" evidence="23">
        <location>
            <begin position="2"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="296"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="347"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00083344"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="415" mass="47453" checksum="F5D86B91E64C1549" modified="2020-06-17" version="1">MPEGPELHLASCYVNKMCDGVVFTGAVRKSEVSKSPDVPFNSEAYRITATSRGKEVKLTLTPIGGDSKRRVKAGQADQPMDIIFRFGMSGFFRLTSEDELPKHSHLRFYSKEKPCRVLSFVDARRFGTWHPNGTWQPDRGPCIMFEYKSFRENVLSHLSDRAFDRPICEVMLNQKYFNGIGNYLRAEILYRLNIPPFVCARTVLEGLESEDLCESEKPVKKEFSDVKGSDRITKVKVKEEKTDLLRLSHTVPLEVVSLGGKGYDPEKADYSGFQAWLQCYSVDGMKSVRDHNGRTMWFQGDSGPMVPKDLKSPKAKKRVKKEDDHDYTDKKKVSRGRSKSTLKRKLIKEETVIKTPKKEKDARLKEAGSKRRKTEKPQEINTPHREKRSTARRRKSSSNDTAAGSQRQSRRMSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CNN4</accession>
    <name>A0A673CNN4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Gamma-aminobutyric acid (GABA) A receptor, alpha 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">gabra5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CNN4"/>
    <dbReference type="FunCoup" id="A0A673CNN4">
        <property type="interactions" value="517"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057164.1">
        <property type="protein sequence ID" value="ENSSORP00005055875.1"/>
        <property type="gene ID" value="ENSSORG00005024910.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CNN4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19038">
        <property type="entry name" value="LGIC_ECD_GABAAR_A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19052">
        <property type="entry name" value="LGIC_TM_GABAAR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000001">
        <property type="entry name" value="Gamma-aminobutyric acid A receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000002">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit alpha-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047024">
        <property type="entry name" value="Gabra-1-6_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025706653" evidence="18">
        <location>
            <begin position="21"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="35"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="249"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="444" mass="49969" checksum="67A6320608694827" modified="2020-06-17" version="1">MTLKNLCPCLLCSLTQDVAGTPKEAELNDNITIFTRILDGLLDGYDNRLRPGLGEKVTEIKTNIFVTSFGPVSDTEMEYTIDVFFRQSWKDERLCFKGPMEMLHLNNLLASNIWTPDTFFLNGKKSIAHNMTTPNKLLRLKDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPVSEVVYTWTKGAAKSVVVAEDGSRLNQYHLIGQTAGTEDIYTSRGTYTVMMAHFYLKRKIGYFVIQTYMPCFMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRSWAWDGKKAMEAQQPKKKDPLALSKKPNNTCSTGVNYTANLTKDASISTISNSTAVQLKPTETKAPDPKKTYNSVSKIDKMSRIVFPVLFGTFNLVYWATYLNREPVIKGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CNS8</accession>
    <name>A0A673CNS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Density-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">denr</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DENR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030006086.1">
        <property type="nucleotide sequence ID" value="XM_030150226.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030006088.1">
        <property type="nucleotide sequence ID" value="XM_030150228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CNS8"/>
    <dbReference type="FunCoup" id="A0A673CNS8">
        <property type="interactions" value="1414"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056270.1">
        <property type="protein sequence ID" value="ENSSORP00005054994.1"/>
        <property type="gene ID" value="ENSSORG00005024593.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430287"/>
    <dbReference type="CTD" id="8562"/>
    <dbReference type="InParanoid" id="A0A673CNS8"/>
    <dbReference type="OrthoDB" id="277199at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000004">
        <property type="entry name" value="density-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005873">
        <property type="entry name" value="DENR_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01159">
        <property type="entry name" value="DRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="116"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="199" mass="22128" checksum="D46070380C8A4006" modified="2020-06-17" version="1">MATTEMESGSSESRSEQGIADPDSKYPLKVLYCGVCSLPTEYCEYMPEPAKCRQWLEKNFPDVFARMTVGPAANAPKQEPGTGDAPPAGEEEEKKKQKRGGRGQIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGLATFDIDLKEAQRFFAQKFSCGASVTAEDEIIIQGDFTDDIIDVIQEKWPEVDDDSIDDLGEVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CP45</accession>
    <name>A0A673CP45_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Cytochrome c oxidase subunit 7C, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Cytochrome c oxidase polypeptide VIIc</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits, encoded in the mitochondrial DNA, and 11 supernumerary subunits, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the cytochrome c oxidase VIIc family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CP45"/>
    <dbReference type="FunCoup" id="A0A673CP45">
        <property type="interactions" value="1353"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055025.1">
        <property type="protein sequence ID" value="ENSSORP00005053758.1"/>
        <property type="gene ID" value="ENSSORG00005024155.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CP45"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00929">
        <property type="entry name" value="Cyt_c_Oxidase_VIIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.49.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.49.10">
        <property type="entry name" value="Cytochrome c oxidase subunit VIIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004202">
        <property type="entry name" value="COX7C/Cox8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036636">
        <property type="entry name" value="COX7C/Cox8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13313">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT VIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02935">
        <property type="entry name" value="COX7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81427">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="63" mass="7129" checksum="B5B3F00149388C9B" modified="2020-06-17" version="1">MLGQAVRRFTTSAVRSSHYAEGPGKNLPFSVDNKWRLLGMMVVFFGSGFAFPFIVVRHQILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CVS4</accession>
    <name>A0A673CVS4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">CUB and Sushi multiple domains 3a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115435868</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CSMD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005057361.1">
        <property type="protein sequence ID" value="ENSSORP00005056068.1"/>
        <property type="gene ID" value="ENSSORG00005024976.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CVS4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="28"/>
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        <property type="match status" value="14"/>
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        <property type="match status" value="5"/>
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    <dbReference type="FunFam" id="2.60.120.290:FF:000001">
        <property type="entry name" value="CUB and sushi domain-containing protein 3 isoform X1"/>
        <property type="match status" value="11"/>
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    <dbReference type="FunFam" id="2.10.70.10:FF:000014">
        <property type="entry name" value="Membrane cofactor protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Complement Module, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Spermadhesin, CUB domain"/>
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        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
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    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
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        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="14"/>
    </dbReference>
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        <property type="entry name" value="Sushi"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
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    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="14"/>
    </dbReference>
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        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
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    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="3432"/>
            <end position="3451"/>
        </location>
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        <location>
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    </feature>
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            <begin position="723"/>
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    </feature>
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        <location>
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        <location>
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        <location>
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    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
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    </feature>
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        <location>
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    <feature type="disulfide bond" evidence="12">
        <location>
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    <feature type="disulfide bond" evidence="12">
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    </feature>
    <feature type="disulfide bond" evidence="12">
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    <feature type="disulfide bond" evidence="12">
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    <feature type="disulfide bond" evidence="12">
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="3505" mass="383178" checksum="405E373103B7A114" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CX01</accession>
    <name>A0A673CX01_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">dnmt3ab</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-cysteinyl-[protein] + S-adenosyl-L-methionine = S-methyl-L-cysteinyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
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            <dbReference type="ChEBI" id="CHEBI:82612"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66545"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
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        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13682"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673CX01"/>
    <dbReference type="Ensembl" id="ENSSORT00005058562.1">
        <property type="protein sequence ID" value="ENSSORP00005057257.1"/>
        <property type="gene ID" value="ENSSORG00005024991.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CX01"/>
    <dbReference type="OrthoDB" id="641149at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="entry name" value="ADDz_Dnmt3a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PWWP_DNMT3A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA (Cytosine-5)-methyltransferase 3A isoform X1"/>
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        <property type="entry name" value="DNA methyltransferase 3 alpha"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ADD"/>
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        <property type="entry name" value="ADD_DNMT3A"/>
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        <property type="entry name" value="C5-Methyltransferase"/>
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        <property type="entry name" value="C5_DNA_meth_AS"/>
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        <property type="entry name" value="C5_MeTfrase"/>
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        <property type="entry name" value="DNM3A_N"/>
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        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 3A"/>
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    </dbReference>
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        <property type="entry name" value="DNA CYTOSINE-5- -METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ADD_DNMT3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNM3A_N"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF21255">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ADD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PWWP" evidence="16">
        <location>
            <begin position="420"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="609"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="102"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="278"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="325"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00050180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00050478"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1039" mass="117867" checksum="EBCD94E5061C7C04" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y7F0</accession>
    <name>A0A672Y7F0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Hydroperoxide isomerase ALOXE3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">zgc:152891</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSSORT00005002449.1">
        <property type="protein sequence ID" value="ENSSORP00005002388.1"/>
        <property type="gene ID" value="ENSSORG00005001421.1"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="13">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="14">
        <location>
            <begin position="131"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="673"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="11">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="673" mass="76099" checksum="F40017CC77B4FEC9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9L1</accession>
    <name>A0A672Y9L1_9TELE</name>
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        <recommendedName>
            <fullName evidence="5">Centriolar coiled-coil protein 110</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">cp110</name>
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    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005000544.1"/>
        <property type="gene ID" value="ENSSORG00005000371.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CENTRIOLAR COILED-COIL PROTEIN OF 110 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SI:CH73-100L22.3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CaM_bind"/>
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    </dbReference>
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        </location>
    </feature>
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        <location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
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        <location>
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        <location>
            <begin position="48"/>
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        </location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="860"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1137" mass="128170" checksum="51B60453047121B0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9N2</accession>
    <name>A0A672Y9N2_9TELE</name>
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        <recommendedName>
            <fullName evidence="11">Myogenic factor</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="primary">myod1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2 11">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030136739.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000615.1">
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        <property type="gene ID" value="ENSSORG00005000401.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A672Y9N2"/>
    <dbReference type="OrthoDB" id="10049614at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of myoblast differentiation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of skeletal muscle fiber development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:skeletal muscle cell differentiation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myogenic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
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        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="Myf5"/>
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        <property type="entry name" value="MyoD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039704">
        <property type="entry name" value="Myogenic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11534:SF2">
        <property type="entry name" value="MYOBLAST DETERMINATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11534">
        <property type="entry name" value="MYOGENIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12232">
        <property type="entry name" value="Myf5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
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    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="109"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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        </location>
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        <location>
            <begin position="48"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="296" mass="32177" checksum="CB2CC49C07B8181E" modified="2020-06-17" version="1">MELSDISFPIPAADDFYDDPCFNTSDMHFFEDLDPRLVHVGLLKPDDSSSSTSSSPSSSSSSPSSLLHLHHHAEVEDDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATLRERRRLSKVNDAFETLKRCTTANPNQRLPKVEILRNAISYIESLQALLRGGQEDGYYPVLEHYSGDSDASSPRSNCSDGMTDFNGPTCQSTRRGSYDNSSYFSETPNGGLKSERSSVVSSLDCLSSIVERISTDTTSLLPAAEGPASPPADPASETAATGPIRVPSPTASQDPNLIYQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YBS3</accession>
    <name>A0A672YBS3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat domain 27 (VPS9 domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ankrd27</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGT3</accession>
    <name>A0A672YGT3_9TELE</name>
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        <recommendedName>
            <fullName evidence="14 15">ATP synthase F(0) complex subunit e, mitochondrial</fullName>
        </recommendedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12 15">Subunit e, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP).</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATPase e subunit family.</text>
    </comment>
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    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
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        <location>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00057306"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00064647"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074682"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003781.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="71" mass="8117" checksum="CC985ACEDA9AC191" modified="2020-06-17" version="1">MAPPVAVSPLIKTARYSALIAGIIYGKRRYDHLKPIAAEERRIEEEEKKAREEQERIAKQLAEANEDTILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YH39</accession>
    <name>A0A672YH39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Soluble calcium-activated nucleotidase 1</fullName>
            <ecNumber evidence="6">3.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Apyrase homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cant1a</name>
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    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ribonucleoside 5'-diphosphate + H2O = a ribonucleoside 5'-phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the apyrase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.6" evidence="6"/>
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        <property type="protein sequence ID" value="ENSSORP00005003873.1"/>
        <property type="gene ID" value="ENSSORG00005002350.1"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:GDP phosphatase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:UDP phosphatase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Soluble calcium-activated nucleotidase 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Apyrase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Apyrase"/>
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        <property type="entry name" value="Apyrase_sf"/>
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    <dbReference type="PANTHER" id="PTHR13023">
        <property type="entry name" value="APYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOLUBLE CALCIUM-ACTIVATED NUCLEOTIDASE 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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        <location>
            <position position="182"/>
        </location>
        <ligand>
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        <location>
            <position position="228"/>
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        <ligand>
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        <location>
            <position position="297"/>
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        <ligand>
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        </ligand>
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        <location>
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        <ligand>
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        <ligand>
            <name>Ca(2+)</name>
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            <dbReference type="ARBA" id="ARBA00077367"/>
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            <dbReference type="PIRSR" id="PIRSR609283-1"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003873.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="414" mass="46268" checksum="52594267E3528B6E" modified="2020-06-17" version="1">MESHLSPSSPMPASPGYAQLDQNEPMSSLRISVGGLPMLASMANTTDPRFRLKWRPIVVVALSLTLILLLFMHLGSGFRSHPSNSWRASRSDHHQSSSHYNDTYPLSPPERTPLGTRYRIGVIADLDTNSLSTKKLTWFSYMRRGHLLVSQSGDKVAVEWDADRVVLESHLAEKGRGMELSELVVFNGKLYSVDDRTGVVYQIDGDTAVPWVILPDGDGSVAKGFKAEWLAVKDEHLYVGGLGKEWTTTEGEFVNNNPEWVKVVGFRGDVRHENWVPKYHALKSAAGIKSPGYLIHESAAWSDTLKHWFFLPRRASNERYEETADERRGTNLALSCSPDFKDITVSRVGELNPTHGFSSFKFVPNTDDQIILALKSEEDAGKIATYIMAFTLDGRILLPETKIGDVKYEGLEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHD0</accession>
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        <recommendedName>
            <fullName evidence="4">Purkinje cell protein 4</fullName>
        </recommendedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the PCP4 family.</text>
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    </dbReference>
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        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="7125" checksum="7A3D0C5EF4F6EA4D" modified="2020-06-17" version="1">CNFCISQIHPQGRTRLFGGLDLKNNVPQDFDIDMDNPETEKAAVAIQSQFRKFQKKKQDVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHG5</accession>
    <name>A0A672YHG5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Bromodomain and PHD finger containing, 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">brpf1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_030138995.1"/>
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    <dbReference type="RefSeq" id="XP_029994856.1">
        <property type="nucleotide sequence ID" value="XM_030138996.1"/>
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    <dbReference type="Ensembl" id="ENSSORT00005004138.1">
        <property type="protein sequence ID" value="ENSSORP00005004020.1"/>
        <property type="gene ID" value="ENSSORG00005002442.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A672YHG5"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ePHD_BRPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15572">
        <property type="entry name" value="PHD_BRPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20156">
        <property type="entry name" value="PWWP_BRPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000008">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000008">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000007">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049583">
        <property type="entry name" value="BRPF1_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042061">
        <property type="entry name" value="Peregrin_ePHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF85">
        <property type="entry name" value="PEREGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="317"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="371"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="710"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="16">
        <location>
            <begin position="1179"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="46"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="141"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="493"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="622"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="810"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="920"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1289"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="524"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="810"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1022"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1091"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1126"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1299"/>
            <end position="1308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1308" mass="146699" checksum="CC4CD9B9712B694C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLK4</accession>
    <name>A0A672YLK4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Poly(rC)-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115419663</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029990436.1">
        <property type="nucleotide sequence ID" value="XM_030134576.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029990437.1">
        <property type="nucleotide sequence ID" value="XM_030134577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YLK4"/>
    <dbReference type="SMR" id="A0A672YLK4"/>
    <dbReference type="FunCoup" id="A0A672YLK4">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005666.1">
        <property type="protein sequence ID" value="ENSSORP00005005480.1"/>
        <property type="gene ID" value="ENSSORG00005002959.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419663"/>
    <dbReference type="InParanoid" id="A0A672YLK4"/>
    <dbReference type="OrthoDB" id="442947at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22515">
        <property type="entry name" value="KH-I_PCBP1_2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22521">
        <property type="entry name" value="KH-I_PCBP1_2_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000002">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000003">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000005">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="96"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="247"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="326" mass="34100" checksum="DDA5B628867736E7" modified="2020-06-17" version="1">MDSGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESGARINISEGNCPERIITLAGPTTAIFKAFSMIIEKLEEDISSSMTNSTATSKPPVTLRIVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGTPQSIIECVKQICVVMLESPPKGVTIPYRPKPSGSPVIFAGGQAYAVQGQHAIPQPDSSSAAISPQLTKLHQLAMQQSPFPIAPSNQGFTGIDASAQTSSHEMTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVDGSTDRQVTITGSPASISLAEYLINARLSSEATGLAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLS1</accession>
    <name>A0A672YLS1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CAAX prenyl protease 2</fullName>
            <ecNumber evidence="11">3.4.26.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Farnesylated proteins-converting enzyme 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115438114</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A672YLS1"/>
    <dbReference type="FunCoup" id="A0A672YLS1">
        <property type="interactions" value="1620"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005747.1">
        <property type="protein sequence ID" value="ENSSORP00005005545.1"/>
        <property type="gene ID" value="ENSSORG00005003323.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLS1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="14">
        <location>
            <begin position="151"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="314" mass="34810" checksum="B37A425BBA304A84" modified="2020-06-17" version="1">KPQDGGHSTLLSTMKHRNGVFVPPEGLCWLSVLSCLLLACSYVGSLYVWRSDLPRDHPAVIKRRFTSVLIVSGLSPLFVWAWREFTGTVPSLLALMGIRFEGLIPAIVLPLLLTMVLFLGPLMQLAMDCPWSFMDGVRVAFGEAANTDMRWLRNQVVAPLTEELVFRACMLPMLVPCAGPFTAIFTCPLFFGVAHFHHVIELLRFRQGTLSGIFLSAVFQFSYTAVFGAYTAFIFIRTGHLMGPVLCHSFCNYMGFPAISTALEHPHRLTVLSSYLLGVLLFLFLLFPFTDPSFYGLPTPVCTLTSSPSSLCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMJ7</accession>
    <name>A0A672YMJ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cell cycle control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115415207</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984568.1">
        <property type="nucleotide sequence ID" value="XM_030128708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YMJ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005006041.1">
        <property type="protein sequence ID" value="ENSSORP00005005794.1"/>
        <property type="gene ID" value="ENSSORG00005003495.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415207"/>
    <dbReference type="InParanoid" id="A0A672YMJ7"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF17">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12 16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="368" mass="41758" checksum="55F63BB22BE411E5" modified="2020-06-17" version="1">MMASTYNAKDEDGHITVAGPGGGTLRSRKPENTAFKQQRLPAWQPILTAGTVLPAFFVIGLIFIPIGIGLYVTSNNIKEFEIDYTGVDMSSPCFNCSQNFSWNSTRPCTCSIPFYLDQPYESNVFMYYGLSNFYQNHRRYVKSRDDSQLNGDSEALTKPSKECEPYAFHDNKPIAPCGAIANSMFNDSLELFYNDPNGTKVQIHLMKTGIAWWTDKHVKFRNPGGNNPNLTVAFQGTTKPVNWRKPVYELDSDQENDGFINEDFIVWMRTAALPTFRKLYRIIQKKKDMVLTLPRGNYTLEVVYNYPVRSFEGRKRMILSTISWMGGKNPFLGIAYITVGSVCFFLGVVLLIIHHKYGNRNNSADISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPT4</accession>
    <name>A0A672YPT4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription factor 19 (SC1), like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115428907</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Potential transcription factor that may play a role in the regulation of genes involved in cell cycle G1/S transition. May bind to regulatory elements of genes, including the promoter of the transcription factor FOXO1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YPT4"/>
    <dbReference type="Ensembl" id="ENSSORT00005006797.1">
        <property type="protein sequence ID" value="ENSSORP00005006540.1"/>
        <property type="gene ID" value="ENSSORG00005003826.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPT4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22685">
        <property type="entry name" value="FHA_TCF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15609">
        <property type="entry name" value="PHD_TCF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042803">
        <property type="entry name" value="TCF19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039095">
        <property type="entry name" value="TCF19_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15464">
        <property type="entry name" value="TRANSCRIPTION FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15464:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="33"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="341" mass="37971" checksum="3A8015D1D73BD5A7" modified="2020-06-17" version="1">MLPCFQLLRIGSSGGDSARDLYTFRPALSQSVFRLGRAAELCDVTLDSASVSRIHAELHAEREGNSDDVVSQEEGWRVHVKDRSSHGTWVNEVRLQPGIQWELSDGDTLVFGGQSAPGSPEFYFLFQKVKVRPLDFDAITIPKAGTFSSDIRDRIRTNLDRKVAANLDLSKLSINRATVILNSIGSLSKMKGSAWTFKRSHSHEGTVSDPVSVPSNKSLPAASRSRRKSAHTVLLEDDSSDDHDQQPTEPPPAAREPRRPRRTQTGSGDHVPGPDRHAREHHMMGGGVEPCAAQRCRLPQQDTVQWIQCDVCDAWYHIDCLHIDRKKLLRDPNADFHCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRX8</accession>
    <name>A0A672YRX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ring1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030002492.1">
        <property type="nucleotide sequence ID" value="XM_030146632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YRX8"/>
    <dbReference type="Ensembl" id="ENSSORT00005007586.1">
        <property type="protein sequence ID" value="ENSSORP00005007310.1"/>
        <property type="gene ID" value="ENSSORG00005004183.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427896"/>
    <dbReference type="InParanoid" id="A0A672YRX8"/>
    <dbReference type="OrthoDB" id="337575at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16739">
        <property type="entry name" value="RING-HC_RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000100">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043540">
        <property type="entry name" value="RING1/RING2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042741">
        <property type="entry name" value="RING1_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1 / RING 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="48"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="150"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="289"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="376"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="289"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="404" mass="44968" checksum="40C27EE7E7FAD1F4" modified="2020-06-17" version="1">MAAPVNIQTPSKTWELSLYELHRSPQEAIMDGTEVAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSRRSLRRDSNFDALISKIYPSRDEYEAHQRRVLERLNRLHNKEALSSSIEEGLRQQARYRSERNHRVKKPTQESDNTTFSGGEDNGDTRSHLSHDSAPSHTPHPQGQTPSEAGPSRKRPRASDDGSVAEADSASPTPPARRHKEGPASEIELVFRPHPQLVHEHDYNQTRYVKTTANATVDHLSKYLALRIALEDGRTDRETEDRGGRGEEGGGDTGGTVATGGGGGGGGGGEGSSLSNISEKQYTIYIMTRGGQFPTLNGSLTLELVNEKYWKVRKPLELYYAPTKDQQQQQQQQQQQQQQKASPPSQREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YT05</accession>
    <name>A0A672YT05_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">herc3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029997711.1">
        <property type="nucleotide sequence ID" value="XM_030141851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YT05"/>
    <dbReference type="FunCoup" id="A0A672YT05">
        <property type="interactions" value="948"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008024.1">
        <property type="protein sequence ID" value="ENSSORP00005007741.1"/>
        <property type="gene ID" value="ENSSORG00005004385.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424522"/>
    <dbReference type="CTD" id="8916"/>
    <dbReference type="InParanoid" id="A0A672YT05"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF18">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
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</entry>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YVY8</accession>
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            <taxon>Apogonidae</taxon>
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            <taxon>Sphaeramia</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="230" mass="26605" checksum="B65813B8717FEF92" modified="2020-06-17" version="1">SDSPSKNSYIGSYYHTVRKLADSARRQISICILPDVVTVYIYVINCISAVVDALKTLQEKIKRLELERKQAQKNYHQFSQDAQKKQQVTALHTVQNQPAAAELGSKLQSAEARCKVLEKQLDYMRKMVENAKKDKNAFMENQVTQHLKKGIKSEKLEKLESEYLKLSRTQTLAEMKLAILEQNLLKEQHERKLVQEKADEVSTLLTLFQRTMREIKCITQKINGIYKFSI</sequence>
</entry>
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        </location>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with CYTH2. Interacts with KPNA2; the interaction is direct. Does not interact with ARL4A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z0Z1"/>
    <dbReference type="FunCoup" id="A0A672Z0Z1">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010862.1">
        <property type="protein sequence ID" value="ENSSORP00005010525.1"/>
        <property type="gene ID" value="ENSSORG00005005689.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0Z1"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04152">
        <property type="entry name" value="Arl4_Arl7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000658">
        <property type="entry name" value="ADP-ribosylation factor-like protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
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    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 15 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="7 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="200" mass="22660" checksum="7F593644EA6AAB1F" modified="2020-06-17" version="1">MGNGLSEQPNFLSSVPFFQSFHIAILGLDSAGKTTVLYRLQFNEFVNTVPTKGFNSEKVKVSLGAHRTVTFHFWDVGGQEKLRPLWKSYTRCTDGIIFVVDSVDAERMEEAKTELHKIAKTSENQGVPLLVVANKQDLRHSLGLDEIEKLLALKELGPATPWHLQPTCAIIGDGLREGLERLHDMILKRRKVLRQQRKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z291</accession>
    <name>A0A672Z291_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115413636</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the G-alpha family. G(q) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z291"/>
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        <property type="gene ID" value="ENSSORG00005005914.1"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003924">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.400.10:FF:000017">
        <property type="entry name" value="Guanine nucleotide-binding protein (G protein), alpha 11a (Gq class)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="guanine nucleotide-binding protein G(Q) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003977">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000654">
        <property type="entry name" value="Gprotein_alpha_Q"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
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    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF368">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN (G PROTEIN), ALPHA 11A (GQ CLASS)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00442">
        <property type="entry name" value="GPROTEINAQ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="49"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="155"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="223"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="248"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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            <end position="320"/>
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        <ligand>
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    </feature>
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        <location>
            <position position="374"/>
        </location>
        <ligand>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00007976"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="402" mass="46937" checksum="E000FDFB38249D69" modified="2020-06-17" version="1">MTLESMMACCLSEEAKESKRINAEIDKQLRRDKRDSRRELKLLLLGTGESGKSTFIKQMRIIHGAGYSDEDKRGFIRLVYQNIFTSMQAMIRATETLKIPYKYEQNRSNAMLVKEVDIEKISGFDQPYIVAIKSLWADPGIQEAYDRRREYQLSDSTKYYLSDIDRVTAEGYVPTQQDVLRVRVPTTGIIEYPFDLENVIFSYLSDLDRIADPNYLPTQQDVLRVRIPTTGIIEYPFDLQSIIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKISYSHLVDYFPEFDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z937</accession>
    <name>A0A672Z937_9TELE</name>
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        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z937"/>
    <dbReference type="Ensembl" id="ENSSORT00005014293.1">
        <property type="protein sequence ID" value="ENSSORP00005013876.1"/>
        <property type="gene ID" value="ENSSORG00005007117.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z937"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002281">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000090">
        <property type="entry name" value="Odd-skipped-related transciption factor 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002169">
        <property type="entry name" value="Zgc:174573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000396">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="zinc finger protein 2 isoform X2"/>
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    </dbReference>
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        <property type="entry name" value="Zinc finger protein 26"/>
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    </dbReference>
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        <property type="entry name" value="Zinc finger protein 33A"/>
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    </dbReference>
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        <property type="entry name" value="Zinc finger protein 44"/>
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    </dbReference>
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        <property type="entry name" value="Zinc finger protein 540"/>
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        <property type="entry name" value="Zinc finger protein 660"/>
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        <property type="entry name" value="zinc finger protein 696"/>
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        <property type="entry name" value="zinc finger protein 850-like"/>
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    </dbReference>
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        <property type="entry name" value="zinc finger protein 879 isoform X1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF189">
        <property type="entry name" value="ZINC FINGER PROTEIN 544-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="36"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="36"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
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    </feature>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1059" mass="122037" checksum="D01D54CCC69DEA33" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9Y7</accession>
    <name>A0A672Z9Y7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z9Y7"/>
    <dbReference type="Ensembl" id="ENSSORT00005013722.1">
        <property type="protein sequence ID" value="ENSSORP00005013312.1"/>
        <property type="gene ID" value="ENSSORG00005006902.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9Y7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17971" checksum="7D437BF379A39EC8" modified="2020-06-17" version="1">MKSLMSRQFLWHLACYTYVFSSFHMLFSDACLLLSECFLSVKNPHPSHLHLYIKYIFSLRVWNGVCFIYFYVKTFFFCPFSHSVLNVSKFMRMHARGCIASSLCNTTETGTLLTAGYTVSRTCCNTDCCNGATAIQIPLTAALGAAVMSNYLSYSLSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAW0</accession>
    <name>A0A672ZAW0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromatin target of PRMT1 protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">chtopa</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZAW0"/>
    <dbReference type="Ensembl" id="ENSSORT00005014413.1">
        <property type="protein sequence ID" value="ENSSORP00005013994.1"/>
        <property type="gene ID" value="ENSSORG00005007171.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZAW0"/>
    <dbReference type="OrthoDB" id="446014at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052656">
        <property type="entry name" value="CTOP_PRMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025715">
        <property type="entry name" value="FoP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48426">
        <property type="entry name" value="CHROMATIN TARGET OF PRMT1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48426:SF1">
        <property type="entry name" value="CHROMATIN TARGET OF PRMT1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13865">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01218">
        <property type="entry name" value="FoP_duplication"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Chromatin target of PRMT1 protein C-terminal" evidence="3">
        <location>
            <begin position="152"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="245" mass="26048" checksum="5CD662837AA0517B" modified="2020-06-17" version="1">MSAASSQKVVLKSTTKVSLNERFTNMLKNKQPTAVSIRATMQQQHLASARNRRLAQQMENRPSVQAALNHKQSLKQRLGKSNIQARLGRPIGALMRGGPSGGRGGMRGMTRGGLRGRSRGGVMRGALSLRGKRMPPGGPMRGRGSAGRVMMRRGGRHRGGATGRGGALSRGAARGGMARGRGGLRGRGFAARGGRGRGRGRGVGRPAVTREQLDNQLDAYMSKTKGHLDAELDAYMAQADPDSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDG0</accession>
    <name>A0A672ZDG0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">septin4b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDG0"/>
    <dbReference type="Ensembl" id="ENSSORT00005015404.1">
        <property type="protein sequence ID" value="ENSSORP00005014970.1"/>
        <property type="gene ID" value="ENSSORG00005007606.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDG0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000064">
        <property type="entry name" value="Septin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="9">
        <location>
            <begin position="104"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="388"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="411"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="472" mass="54097" checksum="98E5024F55222965" modified="2020-06-17" version="1">MHSSLQLCHTSHDANREAEGQDCPHTPSYLRPPVAGSALSDSGSEPSLPHSRSVEFDLPSPVSPTRPRSPWARFDPYDNTEDQDKEYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVNSLFLTDLYKDRKLLNAEERITQTVEITKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKSVADYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHEKVNIVPILAKADTLTPTEVKKKKIKIREEIEQYGIKIYQFPDCDSDEDEEFKQQDLELKESIPFAVIGSNTVVEAKGKRVRGRLYPWGIVEVENSAHCDFVKLRNMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRMVVKERNRNKLTRESGTDFPIPVVSGVTDSETEKLIREKDEEVRQVDHETESHEHHDHGHEHEPEHNSEPLEPSDHHANPETHLHQMVDEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZG93</accession>
    <name>A0A672ZG93_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tpd52 like 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPD52 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZG93"/>
    <dbReference type="Ensembl" id="ENSSORT00005016340.1">
        <property type="protein sequence ID" value="ENSSORP00005015844.1"/>
        <property type="gene ID" value="ENSSORG00005008019.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZG93"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007327">
        <property type="entry name" value="TPD52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19307">
        <property type="entry name" value="TUMOR PROTEIN D52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19307:SF13">
        <property type="entry name" value="TUMOR PROTEIN D54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04201">
        <property type="entry name" value="TPD52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="156" mass="17752" checksum="B48D2788904C0ED9" modified="2020-06-17" version="1">SSSELTVEERENLQAELAQIDDEISTLRQVLLTKEKHAAEIRRQLGLSPLSNIKQNLSKGWQEVQTSSPYLRGKENLSYAGQVTSSALSSVGVAITRRLAEMRCISFSILIMKINFYLMKMLIKVHISIILFSADNHDTFSLRFKYTMTIFHTDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGV1</accession>
    <name>A0A672ZGV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hars</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZGV1"/>
    <dbReference type="FunCoup" id="A0A672ZGV1">
        <property type="interactions" value="1731"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016792.1">
        <property type="protein sequence ID" value="ENSSORP00005016285.1"/>
        <property type="gene ID" value="ENSSORG00005006147.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGV1"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000008">
        <property type="entry name" value="histidine--tRNA ligase, cytoplasmic isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025434479" description="histidine--tRNA ligase" evidence="10">
        <location>
            <begin position="19"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="37"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="335"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="522" mass="58177" checksum="3F87F6448390D40F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKX8</accession>
    <name>A0A672ZKX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exo-alpha-sialidase</fullName>
            <ecNumber evidence="3">3.2.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">neu3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of alpha-(2-&gt;3)-, alpha-(2-&gt;6)-, alpha-(2-&gt;8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.</text>
            <dbReference type="EC" id="3.2.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 33 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.18" evidence="3"/>
    <dbReference type="RefSeq" id="XP_030010688.1">
        <property type="nucleotide sequence ID" value="XM_030154828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZKX8"/>
    <dbReference type="Ensembl" id="ENSSORT00005017632.1">
        <property type="protein sequence ID" value="ENSSORP00005017117.1"/>
        <property type="gene ID" value="ENSSORG00005008595.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433427"/>
    <dbReference type="InParanoid" id="A0A672ZKX8"/>
    <dbReference type="OrthoDB" id="2739686at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004308">
        <property type="term" value="F:exo-alpha-sialidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.10:FF:000002">
        <property type="entry name" value="Neuraminidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011040">
        <property type="entry name" value="Sialidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026856">
        <property type="entry name" value="Sialidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628">
        <property type="entry name" value="SIALIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628:SF23">
        <property type="entry name" value="SIALIDASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13088">
        <property type="entry name" value="BNR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sialidase" evidence="10">
        <location>
            <begin position="43"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="411" mass="46170" checksum="C2AF9C25380C6415" modified="2020-06-17" version="1">MGNTTSKSGSGEEPVKTTLFEQEPSGVTYRIPALIYLRHIHTFLAFAEKRSSPYDHDAKILVMRRGTLKDDGTVQWSSCQELTAAVLPNHRTMNPCPVYEKNSKILFLFFVSILGNTTECRQIITGKNKARLCYVSSSDDGQTWSDVKDLTESVIGETIHKWATFAVGPGHGVQLENGRLIIPAYAYYVPYRWCSFPIPFTVYPRALSIYSEDFGQTWHIGKMLRKKSCECEMAEIIDHEGRSHLYCNARNTRGHRCEALSENSGVYFDKPHVAPELVEPSPSGCQGSVIGFPAPEFVPNDDAESKACGTSLLSPDTQTWLLFMHPTRKSSRRDMGVYLNRSPLHSSGWDRPRIIHNGPSGYSDLAYNGDKDQFSGLMECGKESELEQIAFISFTLNDVMQTGSKKEKRMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZL45</accession>
    <name>A0A672ZL45_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CE051</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimoc1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZL45"/>
    <dbReference type="FunCoup" id="A0A672ZL45">
        <property type="interactions" value="1075"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018416.1">
        <property type="protein sequence ID" value="ENSSORP00005017890.1"/>
        <property type="gene ID" value="ENSSORG00005008895.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZL45"/>
    <dbReference type="OrthoDB" id="6135810at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037657">
        <property type="entry name" value="RIMC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28494:SF1">
        <property type="entry name" value="RAB7A-INTERACTING MON1-CCZ1 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28494">
        <property type="entry name" value="UPF0600 PROTEIN C5ORF51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17716">
        <property type="entry name" value="RIMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="32278" checksum="E0025778C53501B7" modified="2020-06-17" version="1">MADDYRRQGFELERRIFELDIKCSSLRAEKQDDDYLQNASAILDKLKSYYRQGGESSSLSKLLQDYTQVILDITFYEENKLVDQEFPEDCSPFKIQQLLQDLTEPEVLAGRLAPAQEVQSVLGLELLECLYWRRGALLYMYCHTLHQRKQWIKKNKDTFLKCIQEGVRYLMRMLQVRNSVKLNDGVVLHDTATASFLSEGIFSDTHLLTMMYIGEMCFWAVKYEDCSADSMERKEDRLQFRDIGTQILNKYVLACEGPLQGQGWNTENAKEILSILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZMZ6</accession>
    <name>A0A672ZMZ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115411413</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with WDR48.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C19 family. USP12/USP46 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZMZ6"/>
    <dbReference type="FunCoup" id="A0A672ZMZ6">
        <property type="interactions" value="1766"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018342.1">
        <property type="protein sequence ID" value="ENSSORP00005017817.1"/>
        <property type="gene ID" value="ENSSORG00005008858.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZMZ6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000003">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF647">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025688296" description="ubiquitinyl hydrolase 1" evidence="11">
        <location>
            <begin position="17"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="38"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="371" mass="42980" checksum="A73D570D4C21CA0C" modified="2020-06-17" version="1">LVLAHVILSFCPFCFGANASALEKEIGSEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKILAYRSQPRRKENLLTCLADLFHSIANQKRKVGVIPPKKFITRLRKENELFDNYMQQDAHEFLNYLLNTIADLLQEERKQEKTNGRLANGTLDSQNNNSNATPAPTWVHEIFQGTLTNETRCLTCETISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMRVKKLPMILALHLKRFKYMEQLQRYTKLSYRVVFPLELRLFNTSGDATNPERLYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLLFDDDIVEKIDAQAIEEFYGLTSEISKNSESGYILFYQSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQC9</accession>
    <name>A0A672ZQC9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">grik4</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15 19">Postsynaptic cell membrane</location>
            <topology evidence="15 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Presynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZQC9"/>
    <dbReference type="Ensembl" id="ENSSORT00005019145.1">
        <property type="protein sequence ID" value="ENSSORP00005018598.1"/>
        <property type="gene ID" value="ENSSORG00005009171.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQC9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000060">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000072">
        <property type="entry name" value="glutamate receptor ionotropic, kainate 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000059">
        <property type="entry name" value="Glutamate receptor, ionotropic, kainate 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000010">
        <property type="entry name" value="Putative glutamate receptor ionotropic kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 19" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6 19" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 19" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027166088" description="Glutamate receptor" evidence="19">
        <location>
            <begin position="31"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="630"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="20">
        <location>
            <begin position="422"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="21">
        <location>
            <begin position="432"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
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        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="679"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="680"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="728"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="18">
        <location>
            <position position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034107"/>
        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018598.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="772" mass="86624" checksum="762A19E104BAD943" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZR45</accession>
    <name>A0A672ZR45_9TELE</name>
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        <recommendedName>
            <fullName evidence="12">Ubiquitin-conjugating enzyme E2 J2</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="15" type="primary">ube2j2</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2.</text>
    </comment>
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            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 J2"/>
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        <property type="entry name" value="Ub_conjugating_enzyme"/>
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    </dbReference>
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        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
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        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
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            <begin position="230"/>
            <end position="251"/>
        </location>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00073320"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="261" mass="28956" checksum="897066B64CC1E3C2" modified="2020-06-17" version="1">MNNNGNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEKTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDYTKRQLSAQSLAFNLKDKVFCELFPDVVDEMKQKQKAQEELSARTQPLPLPDVVPDGDPQHAHYGLPALNGGAVPLGGANPAPGLQQANRNHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZRC8</accession>
    <name>A0A672ZRC8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ral guanine nucleotide dissociation stimulator-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rgl3a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSSORG00005009303.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="26687at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00153">
        <property type="entry name" value="RA_RalGDS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
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    </evidence>
    <sequence length="80" mass="9300" checksum="1F91089785684BD9" modified="2020-06-17" version="1">INITRKRRRPMLNRAGSDIIKAKRSVRMPLAPLIRRRMRPILARRITRNSVGDTKYFSITSDKNIPEKAKHMISNQSLTS</sequence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZV04</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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        <property type="protein sequence ID" value="ENSSORP00005020799.1"/>
        <property type="gene ID" value="ENSSORG00005010156.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433700"/>
    <dbReference type="CTD" id="80195"/>
    <dbReference type="InParanoid" id="A0A672ZV04"/>
    <dbReference type="OrthoDB" id="9984821at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028110">
        <property type="entry name" value="TMEM254"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34104">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34104:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14934">
        <property type="entry name" value="TMEM254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="123" mass="14139" checksum="3F028935AF5718E5" modified="2020-06-17" version="1">MAKSDGCDYFKRTSNFWIISVTLAVGYFTCIVFAPEKIPFQYLGPFGTFCTYLVENHSELMYKGWWAAFAIHVFESLVALKVCSNKGIHSVSTRCLWFVQTFLFGFASLGHLLKYDPKRPKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVE9</accession>
    <name>A0A672ZVE9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115417525</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZVE9"/>
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        <property type="protein sequence ID" value="ENSSORP00005020387.1"/>
        <property type="gene ID" value="ENSSORG00005009938.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZVE9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF115">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047118189" description="Hexosyltransferase" evidence="11">
        <location>
            <begin position="17"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="348" mass="39982" checksum="5B12C03A015DF538" modified="2025-10-08" version="2">MCIMLFVLCYISDSFSWWESFQLNDRYQKFFNWTTEVYSSPVYQVHPKLSVILNETHATTNPPPTTPPGPQYHVAFPQNYHFSLDQADTCKNKTPFLVLMVPVAPDNVEARNAIRQTWGKAEVVQGEVVLTLFMLGLSGQADVEKQQEKIQHENLQHQDLILSDFLDSYFNLTIKTMVIMDWLATHCNNAAYAMKIDSDMFLNVDNLVITLKKPELPKVNYLTGMLMWNSEVIRLNSSKWYVSEEVYPYPTYPPYPLGAGYVFSNDLPGKLVEISKTITPFNIEDVYIGMCLKRLGLSPSSPPDPSQFNDYHRGYNRCMFSKVITYILNSTEELVNFWTDLKKPGSPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZVZ0</accession>
    <name>A0A672ZVZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Solute carrier family 41 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115419185</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SLC41A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZVZ0"/>
    <dbReference type="FunCoup" id="A0A672ZVZ0">
        <property type="interactions" value="351"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021166.1">
        <property type="protein sequence ID" value="ENSSORP00005020592.1"/>
        <property type="gene ID" value="ENSSORG00005010015.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZVZ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000001">
        <property type="entry name" value="Solute carrier family 41 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000002">
        <property type="entry name" value="Solute carrier family 41, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF23">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="11">
        <location>
            <begin position="171"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="11">
        <location>
            <begin position="385"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="49"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="545" mass="58324" checksum="1D93D7905158414D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWB1</accession>
    <name>A0A672ZWB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Photoreceptor-specific nuclear receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nuclear receptor subfamily 2 group E member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Retina-specific nuclear receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115416798</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Orphan nuclear receptor of retinal photoreceptor cells. Transcriptional factor that is an activator of rod development and repressor of cone development. Binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. Enhances rhodopsin expression. Represses M- and S-cone opsin expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZWB1"/>
    <dbReference type="Ensembl" id="ENSSORT00005022031.1">
        <property type="protein sequence ID" value="ENSSORP00005021386.1"/>
        <property type="gene ID" value="ENSSORG00005010457.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZWB1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06970">
        <property type="entry name" value="NR_DBD_PNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06950">
        <property type="entry name" value="NR_LBD_Tlx_PNR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000022">
        <property type="entry name" value="Nuclear receptor subfamily 2 group E member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000028">
        <property type="entry name" value="Nuclear receptor subfamily 2, group E, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="18">
        <location>
            <begin position="47"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="19">
        <location>
            <begin position="166"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="406" mass="45816" checksum="5F4C2939DF301117" modified="2020-06-17" version="1">MRFSESNVCSNIAWCVQSGCRLGSRRRWCPHTRDNTRPPPGKALSPALVCKVCGDTSSGKHYGIYACNGCSGFFKRSVRRRLIYRCQAGTGMCPVDKAHRNQCQACRLKKCLQAGMNKDAVQNERQPRSTAQVRLDSIDVDPEKEHLATTREPTSSSSSFSSSPQNNHRFMASLMTAETCAKLEPEDVDENIDVTSNEPERASSEYHMALYPSSSENVYETSARLLFMSVKWAKNLPVFSNLPFRDQVILLEEAWSELFLLCAIQWSLPLDSCPLLSLPDLCPGMQGKTSYTSLDLRLLQEAFSRFKALAVDPTEFACLKAVVLFKPETRGLKDPEQVENLQDQSQVMLGQHIRSHYPSQPARFGKLLLLLPSLRFVNSERIELLFFHRTIGNTPMEKLLCDMFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY57</accession>
    <name>A0A672ZY57_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115416075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZY57"/>
    <dbReference type="Ensembl" id="ENSSORT00005022666.1">
        <property type="protein sequence ID" value="ENSSORP00005022011.1"/>
        <property type="gene ID" value="ENSSORG00005010750.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZY57"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF33">
        <property type="entry name" value="C-FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025534925" evidence="2">
        <location>
            <begin position="22"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="226" mass="24728" checksum="C90DE8E0B9DA75AD" modified="2020-06-17" version="1">MFGPQMFDMFLYMVTCSVLRCLPVCPTDVSDEDSISSVVQTLTEQLKHTGLNLLINNAAIVVPAMPGPLSETTKKDMMDVYNTNVAGPFLLSKMLLPLLVKAAETHRPEKDKGSSMSCRRSAIINVSTLAGSIEKCPENFHMAPIYPYRTSKAALNMLTRCQAVDFKVSNVLVTAIHPGWVRTEMGGAQAPLNTQDSVQGMLTVMSSLSDKDSGVLVSWEGERIPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZQ3</accession>
    <name>A0A672ZZQ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">si:dkey-183j2.10</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15 18">Postsynaptic cell membrane</location>
            <topology evidence="15 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 18">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZZQ3"/>
    <dbReference type="Ensembl" id="ENSSORT00005022441.1">
        <property type="protein sequence ID" value="ENSSORP00005021793.1"/>
        <property type="gene ID" value="ENSSORG00005010642.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZQ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NMDARECEPTOR"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PBPe"/>
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    </dbReference>
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    <keyword evidence="3 18" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 18" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 18" id="KW-0675">Receptor</keyword>
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    <keyword evidence="5" id="KW-0732">Signal</keyword>
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    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 18" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="208"/>
            <end position="229"/>
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            <begin position="395"/>
            <end position="413"/>
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        <location>
            <begin position="25"/>
            <end position="376"/>
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    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="20">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
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        <location>
            <position position="102"/>
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        <ligand>
            <name>L-glutamate</name>
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        </ligand>
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        <location>
            <position position="107"/>
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        <ligand>
            <name>L-glutamate</name>
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        </ligand>
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        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
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        <location>
            <position position="267"/>
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        <location>
            <position position="313"/>
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        <ligand>
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    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="17">
        <location>
            <position position="245"/>
        </location>
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    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="17">
        <location>
            <position position="272"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
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        <source>
            <dbReference type="RuleBase" id="RU367118"/>
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        <source>
            <dbReference type="SMART" id="SM00079"/>
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        <source>
            <dbReference type="SMART" id="SM00918"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
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    </evidence>
    <sequence length="461" mass="50703" checksum="5E482C1BD9F4DD16" modified="2020-06-17" version="1">IFIIQCLYINVNALYFLPSTSVESELRITTIKQEPYATSKDSQLEGFCMDLLSEVAKKVGFKYQVHLVKDGNYGRQDDNGNWNGMIGEIVRGEADLAIAPLTLTAAREKAVGMTKPFMQTGISFLLRKDVSEGAGFSDFLTPFTAETWVCILVAYLGTAVCIFIVSRLSPCEWNQPQSEQNRFSLLNSLWYTAGALTLQGAGPHPKGLSGRVVICSWWFFTIVLLAFYFSNLSSSNTPQSSQQIVKGFEDLANQDSTQYGCLAGSSTLAFFKNSNNPVYRRIYEHMERTKSFVSTMDEGVRRAKEGNFAFIGESVSLDLAVARHCELVRAHEVIGMRGYSIATSLGSPIIKNLSVAILQLSEAGELAYLRSKWWASSCLADKAKSVAGQPHSLKGMFLVLSLGLGLGLLLAVLELTSRSRSSAVEQKKSCCTVLTEELSLRFKNSNANKPQEAADKDTEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0Z1</accession>
    <name>A0A673A0Z1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cpm</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase M14 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030148717.1"/>
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    <dbReference type="RefSeq" id="XP_030004578.1">
        <property type="nucleotide sequence ID" value="XM_030148718.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030004579.1">
        <property type="nucleotide sequence ID" value="XM_030148719.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030004580.1">
        <property type="nucleotide sequence ID" value="XM_030148720.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030004582.1">
        <property type="nucleotide sequence ID" value="XM_030148722.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030004583.1">
        <property type="nucleotide sequence ID" value="XM_030148723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A0Z1"/>
    <dbReference type="Ensembl" id="ENSSORT00005022916.1">
        <property type="protein sequence ID" value="ENSSORP00005022258.1"/>
        <property type="gene ID" value="ENSSORG00005010850.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429364"/>
    <dbReference type="CTD" id="1368"/>
    <dbReference type="InParanoid" id="A0A673A0Z1"/>
    <dbReference type="OrthoDB" id="10249045at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000019">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF84">
        <property type="entry name" value="CARBOXYPEPTIDASE M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025351268" description="Peptidase M14 domain-containing protein" evidence="11">
        <location>
            <begin position="17"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="12">
        <location>
            <begin position="20"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="454" mass="51273" checksum="81559D966EFC733D" modified="2020-06-17" version="1">MSPLILFLSFLTPITALEFRYHDNSEIKDYLTQISTSNPNITYLYSIGQSVTGQELWVLALGLSPRRHTVGIPEFKYVANMHGNEVLGRVLLLQLIDDLVRGYRNNEKWALYLLNSTRIHILPTMNPDGFDSSDTDCLFSQGRFNHNGIDLNRNFPDAFAGLRRRQQLDEEKREPEVKAVIDWLRTESFVLSANLHGGAVVASYPYDNSNGGSELVNGASVSPDDDVFIHLAKIYSYKHASMHQGERCRDSRPFLDGITNGYQWYPLEGGMQDYNYVWAQCLELTLEVSCCKYPPVKELPALWTDNREALLAFIQQVHLGVKGRVFDSSGVPVHNAVVEVRGRNNMCPFRTNQHGEYYRLLLPGNYSFTVTYPGHEVLTETLTVPYGPDYYSALKHDFQLRQLAMTTGRTLPTSPSPTCNYTRIVEAGGDVNRLAWTELTAGLGLVMVLRSLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6Q2</accession>
    <name>A0A673A6Q2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A6Q2"/>
    <dbReference type="FunCoup" id="A0A673A6Q2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025703.1">
        <property type="protein sequence ID" value="ENSSORP00005024956.1"/>
        <property type="gene ID" value="ENSSORG00005012023.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A6Q2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="131"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="268" mass="31005" checksum="CA1241B6DAE367BE" modified="2020-06-17" version="1">MCDSFTPVFETLMTYMFMLTYFVEYIILFWGESSDVNSVQRGQICLFASAADLSSTNANLRDLLPTLNKQIWNLTHDRDRLNATLTDMIQELERLRLKQEVSRGNNNHNVFFSPLVIKTLCAPCPEGWKLFNGSCYFFSTVTGSWETARNNCRNKSADLLIINTKEEKDFISGFNRRCWIGLSDREQEGTWTWVDGSPVTLSFWDTGEPNDALEFSGEDCAEMLLYSRWNDLNCNDDRPWICENNLWSCPHKRLQQPHSQTPPQVESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8J1</accession>
    <name>A0A673A8J1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A8J1"/>
    <dbReference type="Ensembl" id="ENSSORT00005025639.1">
        <property type="protein sequence ID" value="ENSSORP00005024897.1"/>
        <property type="gene ID" value="ENSSORG00005011995.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A8J1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="285" mass="28110" checksum="0EDCA39B58E8D8B9" modified="2020-06-17" version="1">VWFLGLVLGSGLVVWFGSLVFGSGLWLRQQKTHRLMEENVSNTGTAATVSDTGTAATVSNTGTAATVSNTGTAATVSDTGTAATVSNTGTGATVSTAATVSNTGTAATVSNTGTAATVSNTGTGATVSSMCTGATVSNTGTEVMVSNTGTAATVSNTGTAATVSNTGTGATVSNTGTGATVSNTGTGATVSHTGTAATVSNTGTAATATQAPERQLATQAPERQLATLALERQLATQAPQQRLATQAPQRQLATQAPQRRLATQAPERQLAACALEQRLATQALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8J8</accession>
    <name>A0A673A8J8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A8J8"/>
    <dbReference type="FunCoup" id="A0A673A8J8">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026374.1">
        <property type="protein sequence ID" value="ENSSORP00005025611.1"/>
        <property type="gene ID" value="ENSSORG00005012311.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A8J8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000044">
        <property type="entry name" value="Protein S100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF134">
        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="101" mass="11502" checksum="4CDFF613FAE3E00C" modified="2020-06-17" version="1">MSKLQNLKMSELQNAMESLVAVFHNYSSKEGDKYKLNKSELKTLLQEELGDFMAVSNDPRRVECILEDLDENGDGEVDFQEFVVLVAALTVACNDFFEDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8M0</accession>
    <name>A0A673A8M0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRELI domain containing protein 3B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115422004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION FACTOR E2F3"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="9">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="24"/>
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            <begin position="209"/>
            <end position="274"/>
        </location>
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        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="24"/>
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        <location>
            <begin position="220"/>
            <end position="229"/>
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            <begin position="246"/>
            <end position="258"/>
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            <dbReference type="ARBA" id="ARBA00010940"/>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="RuleBase" id="RU003796"/>
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    <evidence type="ECO:0000256" key="8">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="35225" checksum="FAAB29E3A0296AD9" modified="2020-06-17" version="1">MAYKISNTESSSSNTEERKSHEFCERSRNDTALAALTRRFMELVKRSENGSLDLNVASLLLNAPKRRIYDIINVLEGICLLKKRSKNYIEWAGGQGNILRDAQLMTLIKEEEKLDQLIDRSRRQLRRVFKEHTQRFAYLTYEDVKNIPSLKEQTVIVIKAPAETKVEVPHPGKSLQVHLSSTQGPIDVFVCADDPMPEDCGCLANASTDGDNSIDPVPYSSTVQVSSTDDVSHNTGLSRDSILQPEPTQHSSPVSVTPFSPLLPTSEDQQSFVTLSPPQPFSLDGVGYFQSLVKDEGITDLFSAVDPDLAPLDLDMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AG79</accession>
    <name>A0A673AG79_9TELE</name>
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        <submittedName>
            <fullName evidence="5">Protein phosphatase 4, regulatory subunit 4</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">ppp4r4</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="term" value="C:protein serine/threonine phosphatase complex"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="entry name" value="PPP4R4"/>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4 PPP4R4"/>
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    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4"/>
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    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
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        <location>
            <begin position="17"/>
            <end position="766"/>
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    </feature>
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            <begin position="735"/>
            <end position="756"/>
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            <begin position="186"/>
            <end position="224"/>
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        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="766" mass="87392" checksum="B632B393ACA87CA3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHK9</accession>
    <name>A0A673AHK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Regulator of G-protein signaling 20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization. Interacts with OPRM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AHK9"/>
    <dbReference type="Ensembl" id="ENSSORT00005028619.1">
        <property type="protein sequence ID" value="ENSSORP00005027822.1"/>
        <property type="gene ID" value="ENSSORG00005013315.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AHK9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000015">
        <property type="entry name" value="Regulator of G-protein signaling 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.196.10:FF:000001">
        <property type="entry name" value="Regulator of G-protein signaling 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF145">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RGS" evidence="15">
        <location>
            <begin position="15"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="141" mass="16626" checksum="A3D1768EAAC13D2E" modified="2020-06-17" version="1">HTKPSMEEIRLWSQSFDKLMRNPAGRNVFREFLRTEYSEENMLFWLACEDLKQEINKSAIEEKARSIYEDYISILSPKEVSLDARVREVINKKMQDPTPHTFEDAQLQIYTLMHRDSYPRFLSSSIYKTLVHGGSRTSSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIU9</accession>
    <name>A0A673AIU9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Si:ch73-380l3.2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115435003</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AIU9"/>
    <dbReference type="Ensembl" id="ENSSORT00005030473.1">
        <property type="protein sequence ID" value="ENSSORP00005029640.1"/>
        <property type="gene ID" value="ENSSORG00005014142.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AIU9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484:SF7">
        <property type="entry name" value="MYELIN-ASSOCIATED GLYCOPROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484">
        <property type="entry name" value="SI:CH211-171H4.5-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025581301" evidence="3">
        <location>
            <begin position="22"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="154"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="303" mass="34445" checksum="469B089598273E95" modified="2020-06-17" version="1">LGGSRSIVHKVLFMQLSFVIAVCTDGWKVSVVESLDALVSSCVVLPCTFSHPHENLPTSKLRGKWHVTSDRDQLIYYEDSTRVLENFRGRTKLLGKLGDGNCTLEMIEVKEHDSGPFCFRIEIARTPTDTSTPDKFSFVKNCVTLNMIHEPPKPSLAHKIPVEGYPFTITCSVTHTCPSHIPKLTWSRGSENEITEHHRKLQLGMWETQSILTFIPKEDDDHKDVTCTAEFNGQRTSEETMTLFVKRAVNYNHIIIPTVVAIITAGIFGGLCIFMMKKYKKRINELQSQDGTMWNRMSRISRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKR5</accession>
    <name>A0A673AKR5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mex-3 RNA binding family member D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115436931</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AKR5"/>
    <dbReference type="Ensembl" id="ENSSORT00005029755.1">
        <property type="protein sequence ID" value="ENSSORP00005028937.1"/>
        <property type="gene ID" value="ENSSORG00005013816.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AKR5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22423">
        <property type="entry name" value="KH-I_MEX3_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22424">
        <property type="entry name" value="KH-I_MEX3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16518">
        <property type="entry name" value="RING-HC_MEX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000013">
        <property type="entry name" value="Mex-3 RNA-binding family member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000090">
        <property type="entry name" value="Mex-3 RNA-binding family member C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000012">
        <property type="entry name" value="Mex-3 RNA-binding family member D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047228">
        <property type="entry name" value="KH-I_MEX3_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047226">
        <property type="entry name" value="KH-I_MEX3_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047227">
        <property type="entry name" value="MEX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285">
        <property type="entry name" value="RING FINGER AND KH DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285:SF3">
        <property type="entry name" value="RNA-BINDING PROTEIN MEX3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="440"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="317"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="491" mass="51782" checksum="7F37A56CAE01F29C" modified="2020-06-17" version="1">MPSPLFHPEIMDHDVVISSQHNGVGLPRDTDQESREEDHQEALRFALDQLSLMALEKGPGSESCNGVGGGGYVDLQMLDHPSGSRDSPTSCSPSPEYYGSGGYHMAGSHSMLHGEQSSVLCSRKRSVNMTECVPVPSSEHVAEIVGRQGCKIKALRAKTNTYIKTPVRGEEPVFIVTGRREDVEMAKREIVSAAEHFSMIRASRCKAGGPGGGGSLPGPPHLPGQTTIQVRVPYRVVGLVVGPKGATIKRIQQQTHTYIVTPSREKDPVFEVTGMPENVDRAREEIETHITLRTGTFVDLQGDNDFHTNGTDVSLEGLGALSGGPTSPFSTGSSSAGGGFTFGGDSTPGLPSDELGFEFSASNIWAPFVNGTGNKTASSSQQQPLRRNSSGLSGGAITPRLSPTLSGVGLSSGMLSRLKGSSAPGVAALVGVSPGVNRDCFVCFESEVTAALVPCGHNLFCMECAGQICQSAEPECPVCHTPTTQCIRIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AL19</accession>
    <name>A0A673AL19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">chymotrypsin</fullName>
            <ecNumber evidence="9">3.4.21.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115435010</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.1" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673AL19"/>
    <dbReference type="FunCoup" id="A0A673AL19">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030191.1">
        <property type="protein sequence ID" value="ENSSORP00005029358.1"/>
        <property type="gene ID" value="ENSSORG00005014012.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AL19"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000176">
        <property type="entry name" value="Chymotrypsinogen A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000181">
        <property type="entry name" value="Chymotrypsinogen A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250:SF66">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE PROTEASE CTRL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250">
        <property type="entry name" value="CHYMOTRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025434864" description="chymotrypsin" evidence="11">
        <location>
            <begin position="19"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="34"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="263" mass="28338" checksum="FBCF17C9D98C2980" modified="2020-06-17" version="1">TAMFLLVSCFALVVSALGCGVPAIRPQVSGYNKIVNGETAVSGSWPWQVSLQDGRGFHFCGGSLINQYWVVTAAHCRVSPRSHRVILGEHDRQYNSEQIQVKSISRAITHPYYNTQNFNNDITLLKLSSPVQMSSRISPVCLASSSTSIPSGTRCVTTGWGRTGQTSSPRFLQQTALPLLSPAQCKQYWGSNRITDAMICAGASGVSSCQGDSGGPLVCENGGVWSLVGIVSWGTSNCNVRAPAVYARVSYLRRWIDQTVASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALR0</accession>
    <name>A0A673ALR0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Growth hormone-regulated TBC protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">grtp1a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ALR0"/>
    <dbReference type="FunCoup" id="A0A673ALR0">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030155.1">
        <property type="protein sequence ID" value="ENSSORP00005029322.1"/>
        <property type="gene ID" value="ENSSORG00005013995.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALR0"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000029">
        <property type="entry name" value="Growth hormone-regulated TBC protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000016">
        <property type="entry name" value="TBC1 domain family member 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF10">
        <property type="entry name" value="GROWTH HORMONE-REGULATED TBC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="77"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="346" mass="40115" checksum="B9F859FBB9CB142C" modified="2020-06-17" version="1">MEKSSRSQPDGRAKDRVGSVDPYGFERSEDFDYESYEELMSEYLVVLTRRSIKWSKLLKGKSKVHKNVKVKRYVRKGVPNEHRALIWMTASGAQDQLEKNPNYYQSLLGAQHDPKLVETIRTDLNRTFPDNINFRKTSSPCMQKSLYNVLRAYGHHNPSVGYCQGMNFIAGYLLIITKDEEKSFWLMDALIGRILPDYYSPAMLGLKTDQEVLGELVRLKLPGVWQTMVDHNVMWTLVVSRWFICLYIDILPVETVLRIWDCLFYEGSKILFRVALTLIHHHQAQIQQAQSLPDICQIFKQITHGPFVDECHIFMQKIFTEPGSLSMTTLAKLRETCRARIIAEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQ02</accession>
    <name>A0A673AQ02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4476 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">proser1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030009694.1">
        <property type="nucleotide sequence ID" value="XM_030153834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AQ02"/>
    <dbReference type="FunCoup" id="A0A673AQ02">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032370.1">
        <property type="protein sequence ID" value="ENSSORP00005031491.1"/>
        <property type="gene ID" value="ENSSORG00005014990.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432825"/>
    <dbReference type="CTD" id="80209"/>
    <dbReference type="InParanoid" id="A0A673AQ02"/>
    <dbReference type="OrthoDB" id="5968166at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028011">
        <property type="entry name" value="DUF4476"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042616">
        <property type="entry name" value="PROSER1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14880">
        <property type="entry name" value="PROLINE AND SERINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14880:SF2">
        <property type="entry name" value="PROLINE AND SERINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14771">
        <property type="entry name" value="DUF4476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4476" evidence="2">
        <location>
            <begin position="31"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="464"/>
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        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
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        <location>
            <begin position="501"/>
            <end position="511"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
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        <location>
            <begin position="778"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="798" mass="81244" checksum="44EE09B3C93C38AC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQF6</accession>
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        <recommendedName>
            <fullName evidence="2">DUF4585 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">c18h4orf54</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSSORT00005032388.1">
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        <property type="gene ID" value="ENSSORG00005014997.1"/>
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    <dbReference type="InParanoid" id="A0A673AQF6"/>
    <dbReference type="Proteomes" id="UP000472271">
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    </dbReference>
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        <property type="entry name" value="CEFIP"/>
    </dbReference>
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        <property type="entry name" value="DUF4585"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33775">
        <property type="entry name" value="CARDIAC-ENRICHED FHL2-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33775:SF4">
        <property type="entry name" value="CHROMOSOME 4 OPEN READING FRAME 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15232">
        <property type="entry name" value="DUF4585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="1373"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="287"/>
        </location>
    </feature>
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            <end position="576"/>
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    </feature>
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            <begin position="1190"/>
            <end position="1278"/>
        </location>
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        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
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            <end position="135"/>
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            <end position="1237"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF15232"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1489" mass="162911" checksum="EAEC932875CE2A10" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQV6</accession>
    <name>A0A673AQV6_9TELE</name>
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        <recommendedName>
            <fullName evidence="21">Receptor-type tyrosine-protein phosphatase U</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Receptor-type protein-tyrosine phosphatase psi</fullName>
        </alternativeName>
    </protein>
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        <name evidence="30" type="primary">ptprub</name>
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    <organism evidence="30 31">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="20">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.</text>
    </comment>
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    <dbReference type="FunCoup" id="A0A673AQV6">
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    <dbReference type="Ensembl" id="ENSSORT00005031717.1">
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        <property type="gene ID" value="ENSSORG00005009392.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 16"/>
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        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="MAM_dom"/>
    </dbReference>
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        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
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    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
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        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF10">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE U-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="fn3"/>
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    </dbReference>
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        <property type="entry name" value="Fn3_PTPRU"/>
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    </dbReference>
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        <property type="entry name" value="ig"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
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        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
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    <sequence length="1448" mass="162749" checksum="C1A368E278858898" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="14">Si:dkeyp-69b9.6</fullName>
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        <name evidence="14" type="primary">si:dkeyp-69b9.6</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1515" mass="161705" checksum="50559DA68FC33BE4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATC2</accession>
    <name>A0A673ATC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ATC2"/>
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        <property type="protein sequence ID" value="ENSSORP00005031562.1"/>
        <property type="gene ID" value="ENSSORG00005015017.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATC2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:phosphoric diester hydrolase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP_endonuc_1"/>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
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        <property type="entry name" value="AP ENDONUCLEASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="47270" checksum="86384667CC90ECA4" modified="2020-06-17" version="1">MLAVFPGLFRFCSHFFNIYKISIWNFQSSNNMAKRKSVINSLKRNKIQIAYLQETHLNVEEHKKYCREWVGHIFFSSHSTSKRDLIIHLHKNLPFTATASFKDTEGRYVLVKEILHGENIILGNVYAPNIQDEAFYASLFSQLANMDSPNMIIGDDFNCALCSVMNRLPSQTSKSKNAKAVLNINKEFDLRDIWRHYSPTSKQYTFHSHPYHSASYIGHITLSDHAPVIMGMQLLRPNDRLFSWRMNTTLLTNDKFIKYLTDQTDLFLETNDKNRADPKIRKKEQAAKQVEIEKKKQLEEMFYTTKSDAAVIELKSTITPLYNMIKRKAEIDTLFTKQKFFELANKPNRLLVLLFLWILKRHLIELSGNIHYRSKVLEHPNFSSFVLKFKPFKSNEQLEMVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATE6</accession>
    <name>A0A673ATE6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">AT rich interactive domain 4A (RBP1-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">arid4a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ATE6"/>
    <dbReference type="Ensembl" id="ENSSORT00005033516.1">
        <property type="protein sequence ID" value="ENSSORP00005032619.1"/>
        <property type="gene ID" value="ENSSORG00005015475.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATE6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18641">
        <property type="entry name" value="CBD_RBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20390">
        <property type="entry name" value="Tudor_ARID4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20459">
        <property type="entry name" value="Tudor_ARID4A_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000012">
        <property type="entry name" value="AT-rich interactive domain-containing protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000003">
        <property type="entry name" value="AT-rich interactive domain-containing protein 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000009">
        <property type="entry name" value="AT-rich interactive domain-containing protein 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051232">
        <property type="entry name" value="ARID/SWI1_ChromRemod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012603">
        <property type="entry name" value="ARID4A/B_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047473">
        <property type="entry name" value="CBD_RBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047472">
        <property type="entry name" value="Tudor_ARID4A_rpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13964:SF26">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13964">
        <property type="entry name" value="RBP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08169">
        <property type="entry name" value="RBB1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="ARID" evidence="11">
        <location>
            <begin position="317"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="126"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="465"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="604"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="508"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="605"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="700"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="802"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="845"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="930"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="984"/>
            <end position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1022" mass="114183" checksum="DCDA8121DA4E69B9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVD1</accession>
    <name>A0A673AVD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], mitochondrial</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aldehyde dehydrogenase family 6 member A1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">aldh6a1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Malonate and methylmalonate semialdehyde dehydrogenase involved in the catabolism of valine, thymine, and compounds catabolized by way of beta-alanine, including uracil and cytidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(R)-2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:141212"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:76624"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76627"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62413"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:76628"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20804"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:20805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-oxopropanoate + NAD(+) + CoA + H2O = hydrogencarbonate + acetyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76615"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:76616"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673AVD1"/>
    <dbReference type="FunCoup" id="A0A673AVD1">
        <property type="interactions" value="1643"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034196.1">
        <property type="protein sequence ID" value="ENSSORP00005033289.1"/>
        <property type="gene ID" value="ENSSORG00005015390.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AVD1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="48"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="535" mass="58407" checksum="F941692EF2CD944C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVH4</accession>
    <name>A0A673AVH4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="2 6">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMT family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="2 6"/>
    <dbReference type="AlphaFoldDB" id="A0A673AVH4"/>
    <dbReference type="Ensembl" id="ENSSORT00005033220.1">
        <property type="protein sequence ID" value="ENSSORP00005032333.1"/>
        <property type="gene ID" value="ENSSORG00005015337.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AVH4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF14">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015892">
        <property type="entry name" value="N-myristl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00975">
        <property type="entry name" value="NMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00976">
        <property type="entry name" value="NMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="8">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="9">
        <location>
            <begin position="132"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="303" mass="35739" checksum="2EDBC1AF4AD787FA" modified="2020-06-17" version="1">MWDTLDLSNADVLKEVYTLLNENYVEDDDNMFRFDYSPNFLKWALRPPGWLPQWHCGVRDIRIYDTLKRMVEINFLCVHKKLRSKRVAPVLIREITRRVNLEGIFQAVYTAGVVWTPFFCVFRYWHRSLNPRNRNMTLQRTMKLYRLPDSTKTPGLRPMEKRDISQVTELLRKYLKRFQLAPSMGEEEVAHWFSPQDNIIDTYVVEGADGVLTDFASFYTLPSTVMHHPLHRSLKAAYSFYNVHTQTPLLDLMNDALSLKGFDVFNALDLMENKVFLEKLKFGIGDGNLQYYLYNWKCPPYGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVV6</accession>
    <name>A0A673AVV6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Interferon-related developmental regulator 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ifrd2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IFRD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AVV6"/>
    <dbReference type="FunCoup" id="A0A673AVV6">
        <property type="interactions" value="1242"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033392.1">
        <property type="protein sequence ID" value="ENSSORP00005032502.1"/>
        <property type="gene ID" value="ENSSORG00005015035.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AVV6"/>
    <dbReference type="OrthoDB" id="18978at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039777">
        <property type="entry name" value="IFRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006921">
        <property type="entry name" value="Interferon-rel_develop_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007701">
        <property type="entry name" value="Interferon-rel_develop_reg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354:SF8">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05004">
        <property type="entry name" value="IFRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04836">
        <property type="entry name" value="IFRD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon-related developmental regulator N-terminal" evidence="4">
        <location>
            <begin position="57"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon-related developmental regulator C-terminal" evidence="3">
        <location>
            <begin position="401"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="456" mass="50145" checksum="3C0F36507112A506" modified="2020-06-17" version="1">MPRSKKGKRGSGKPGSLRQEVLQLQLSAKASLSTKGVKNGIKGESGASDDELTSDVLSHYSSASETASVVDEGTGGEPVDEQTAQEETEDKLKQCIDNLMDKSAKTRLAALESLRQAFCSKVLYEFLSERRLTVSDCLERSLKKGGGEEQAAAATVFTLLCIQLGGGDEAEVGFNMLRPLLSNILIDSGASLAARQSCARALGMCCYVSAGDDGEDLIKSLALLESVFTSSYPNREGVLPTPKPGTPGLHAAALQAWALLVTLCPASRLLVLLDLHLPKLEACLQSSDVHFRIAVGETIALLVELGRDVDEEFEVDNSDSLCDCLKGLATDGNKHRAKNDRRKQRSIFREVLHYIENDDFTEEKIRFGVESVYIDGWMRRRIYDAFKEILESGVRHHLQFNPLLRDIFGLGPPLILDANIRTNKISRFEKHLFNSAAFKARTKQRSKVRDKRADVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AY49</accession>
    <name>A0A673AY49_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">rnf34b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 23">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673AY49"/>
    <dbReference type="Ensembl" id="ENSSORT00005035649.1">
        <property type="protein sequence ID" value="ENSSORP00005034726.1"/>
        <property type="gene ID" value="ENSSORG00005016366.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AY49"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16706">
        <property type="entry name" value="RING-HC_CARP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.140:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000110">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049320">
        <property type="entry name" value="CARP1_2_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055111">
        <property type="entry name" value="RNF34_RFFL_HeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057299">
        <property type="entry name" value="RNF34_RFFL_SAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF17">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21272">
        <property type="entry name" value="FYVE_CARP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22968">
        <property type="entry name" value="RNF34L-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23632">
        <property type="entry name" value="SAP_RNF34_RFFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="155"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="161"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="403" mass="44623" checksum="9CAA644FD7728306" modified="2020-06-17" version="1">MKAGASSMWASCCGLLNEVMGTGTVRTQQPGFGAGAGPFRFAPSAGYSTYPPTSSGSAGQLCKACGLAFSVFRRKHICCDCKKSFCALCSVLQENLRCCTTCHLLRGTAFQRPRLMQLRVKDLRQYLLLRNIPTDTCREKEDLVDLVLCHQGAGHTPGAVVEEEEEEDDDDEEEEEEETTHEEEEEEEEEEGDDEGGDDIDNEHLLPQSEAASPVCATRSNSEQSLLSASQGEVISPSSSSGTSNQEHEDTPTASLLNLEPTENMIEVSPATQRRIRASLSDLDNEEAIENLSVRQLKEILARNFVNFSGCCEKWELLERVHRLYRENQQNRKSMENVSITADGVKAQLASDENLCRICMDAIIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AY85</accession>
    <name>A0A673AY85_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cct5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the chaperonin-containing T-complex (TRiC), a hexadecamer composed of two identical back-to-back stacked rings enclosing a protein folding chamber. Each ring is made up of eight different subunits: TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A/CCT6, CCT7, CCT8. Interacts with PACRG. Interacts with DNAAF4. Interacts with DLEC1. Interacts with SPMAP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029978819.1">
        <property type="nucleotide sequence ID" value="XM_030122959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AY85"/>
    <dbReference type="FunCoup" id="A0A673AY85">
        <property type="interactions" value="2186"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034229.1">
        <property type="protein sequence ID" value="ENSSORP00005033322.1"/>
        <property type="gene ID" value="ENSSORG00005015781.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411034"/>
    <dbReference type="CTD" id="22948"/>
    <dbReference type="InParanoid" id="A0A673AY85"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000053">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000049">
        <property type="entry name" value="T-complex protein 1 subunitTheta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="541" mass="59625" checksum="C8F87BAD08ADCDC8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4P5</accession>
    <name>A0A673B4P5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCAAT/enhancer-binding protein zeta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCAAT-box-binding transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cebpz</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Stimulates transcription from the HSP70 promoter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B4P5"/>
    <dbReference type="FunCoup" id="A0A673B4P5">
        <property type="interactions" value="1919"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036610.1">
        <property type="protein sequence ID" value="ENSSORP00005035657.1"/>
        <property type="gene ID" value="ENSSORG00005016834.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B4P5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000805">
        <property type="entry name" value="Similar to transcription factor CBF/MAK21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="12">
        <location>
            <begin position="504"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="87"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="743"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="794"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="831"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="749"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="794"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="804"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="854"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="895"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1010" mass="114376" checksum="EEB36FC18DA7E56B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B548</accession>
    <name>A0A673B548_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acyl-CoA thioesterase 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115421255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B548"/>
    <dbReference type="Ensembl" id="ENSSORT00005038375.1">
        <property type="protein sequence ID" value="ENSSORP00005037401.1"/>
        <property type="gene ID" value="ENSSORG00005017560.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B548"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2240:FF:000002">
        <property type="entry name" value="Acyl-CoA thioesterase 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000024">
        <property type="entry name" value="acyl-coenzyme A thioesterase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2240">
        <property type="entry name" value="Acyl-CoA thioester hydrolase/BAAT N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016662">
        <property type="entry name" value="Acyl-CoA_thioEstase_long-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014940">
        <property type="entry name" value="BAAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006862">
        <property type="entry name" value="Thio_Ohase/aa_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042490">
        <property type="entry name" value="Thio_Ohase/BAAT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824:SF36">
        <property type="entry name" value="ACYL-COA THIOESTERASE 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08840">
        <property type="entry name" value="BAAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04775">
        <property type="entry name" value="Bile_Hydr_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016521">
        <property type="entry name" value="Acyl-CoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="BAAT/Acyl-CoA thioester hydrolase C-terminal" evidence="4">
        <location>
            <begin position="214"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR016521-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="47714" checksum="3E69E0F3425C8B21" modified="2020-06-17" version="1">MDKKHCVKLSVQPSRALVDEKFIVLVQNAPPGFQMTIHSLHHSEDGHSWEAFGHYTADATGTVNVSQDHSVGGTYSGVEPMGLLWSLRPVPGSKSWLRFRKMNVETPMEVAISVYQGHQTVFVDQVQLASVVAERWYMAPGVRRIPVTESDLTATLFLPPGPGPFPGVLDLWGGAGQLVEYRSALLASHGFASLALDYLTPKITLKTGKMVKDDYFETAYKFLQQHPKVSGCRIAMMGLSYSTSITLKMAIYSEVMKLSCAVCTSGSHVQPVNGSVEDIFKMFHSNSEKARFTEEKQAIYRDLLLPIPTDPGMKLDVSLTIPCPLLLIVGEDDQNWAAPEFAQDIKEMMEQAGNSHLLTLLSYPNTGHLIEPPYLPHARASRFKSVVVVLWGGDTVAHSRAQEDAWRKTLAFLRENLYSRKNSIAPVREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5J6</accession>
    <name>A0A673B5J6_9TELE</name>
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        <recommendedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit 37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Leucine-rich repeat-containing protein 68</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ppp1r37</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP1R37 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030007617.1">
        <property type="nucleotide sequence ID" value="XM_030151757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B5J6"/>
    <dbReference type="FunCoup" id="A0A673B5J6">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037570.1">
        <property type="protein sequence ID" value="ENSSORP00005036604.1"/>
        <property type="gene ID" value="ENSSORG00005016892.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431426"/>
    <dbReference type="CTD" id="284352"/>
    <dbReference type="InParanoid" id="A0A673B5J6"/>
    <dbReference type="OrthoDB" id="10034042at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF9">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 37"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0650">Protein phosphatase inhibitor</keyword>
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            <end position="175"/>
        </location>
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        </location>
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        </location>
    </feature>
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            <end position="685"/>
        </location>
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            <begin position="720"/>
            <end position="730"/>
        </location>
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        <location>
            <begin position="1117"/>
            <end position="1129"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038315"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040684"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1198" mass="130452" checksum="41E37EE0323E5059" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6U5</accession>
    <name>A0A673B6U5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B6U5"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="31"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="146" mass="16938" checksum="56CECED77ACBD65C" modified="2020-06-17" version="1">RAALIYVSYVVFYSPSGCGLIACSKIPVRQYHYVNMSMSWSDAQRYCREKYTDLATFENMEDIERLNRPGLLTTWAWIGLRDDPKAWTGTMGNDGNSWRWSANHQITGFHNWKSGEPNYASKLETFALTQCIRKFSFPLKFYPPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAW6</accession>
    <name>A0A673BAW6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SH3 domain-binding glutamic acid-rich-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">zgc:153284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3BGR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BAW6"/>
    <dbReference type="Ensembl" id="ENSSORT00005038578.1">
        <property type="protein sequence ID" value="ENSSORP00005037598.1"/>
        <property type="gene ID" value="ENSSORG00005017644.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BAW6"/>
    <dbReference type="OrthoDB" id="9932926at2759"/>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glut_rich_SH3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051033">
        <property type="entry name" value="SH3BGR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232:SF15">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04908">
        <property type="entry name" value="SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="91" mass="10050" checksum="07C9CAEBAADB1C46" modified="2020-06-17" version="1">MSVKVFYTSVSGSVEMKKRQERILSVLSSTGVTFDLIDISQDSANKEKMRRIAGNPTALAPQIANGDTYCGDFEAFENAIENGELQKFLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BBL3</accession>
    <name>A0A673BBL3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BBL3"/>
    <dbReference type="Ensembl" id="ENSSORT00005039659.1">
        <property type="protein sequence ID" value="ENSSORP00005038664.1"/>
        <property type="gene ID" value="ENSSORG00005018066.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BBL3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF299">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="179" mass="19957" checksum="009E3D4F4BB0CCC1" modified="2020-06-17" version="1">QRGPTCTPKGDRGLSCFRLGHENLCEHSLHLHEGLDTGATASLTGTLPHSLPSTPDIENAELTPILPFLYLGNEHDAQDINLLQRLNIGYILNVTTHLPLYHYDTGLFIYKRLPATDSNKQNLVQHNSKSYGQILMKFSGFVGNGPPRGPPHPRSPPKFNHFFLIPFPTNPENFIQICP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BGB2</accession>
    <name>A0A673BGB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Contactin associated protein-like 5a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115412764</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BGB2"/>
    <dbReference type="Ensembl" id="ENSSORT00005041863.1">
        <property type="protein sequence ID" value="ENSSORP00005040810.1"/>
        <property type="gene ID" value="ENSSORG00005019050.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BGB2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000026">
        <property type="entry name" value="contactin-associated protein-like 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF84">
        <property type="entry name" value="CONTACTIN-ASSOCIATED PROTEIN-LIKE 5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="F5/8 type C" evidence="12">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="13">
        <location>
            <begin position="93"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="13">
        <location>
            <begin position="276"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="458"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="15">
        <location>
            <begin position="494"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="13">
        <location>
            <begin position="704"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="870"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="13">
        <location>
            <begin position="931"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="842"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1063" mass="118678" checksum="894492FB5F5958FD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKI4</accession>
    <name>A0A673BKI4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nuclear transcription factor, X-box binding-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nfxl1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFX1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BKI4"/>
    <dbReference type="FunCoup" id="A0A673BKI4">
        <property type="interactions" value="1452"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044234.1">
        <property type="protein sequence ID" value="ENSSORP00005043141.1"/>
        <property type="gene ID" value="ENSSORG00005019216.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BKI4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd16697">
        <property type="entry name" value="RING-CH-C4HC3_NFXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF1">
        <property type="entry name" value="NF-X1-TYPE ZINC FINGER PROTEIN NFXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="854"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="151"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="821"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="825"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="881" mass="97340" checksum="155942FF9072892D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BL39</accession>
    <name>A0A673BL39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Ephrin type-A receptor 7</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">epha7</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673BL39"/>
    <dbReference type="FunCoup" id="A0A673BL39">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005042026.1">
        <property type="protein sequence ID" value="ENSSORP00005040973.1"/>
        <property type="gene ID" value="ENSSORG00005017514.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BL39"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05066">
        <property type="entry name" value="PTKc_EphR_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09548">
        <property type="entry name" value="SAM_EPH-A7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000045">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000130">
        <property type="entry name" value="Ephrin type-A receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000190">
        <property type="entry name" value="Ephrin type-A receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF9">
        <property type="entry name" value="EPHRIN TYPE-A RECEPTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
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    </dbReference>
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        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="13 23 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11 23 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 27" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025573947" description="Ephrin type-A receptor 7" evidence="27">
        <location>
            <begin position="27"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="546"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="31">
        <location>
            <begin position="31"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="30">
        <location>
            <begin position="330"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="30">
        <location>
            <begin position="441"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="28">
        <location>
            <begin position="620"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="29">
        <location>
            <begin position="928"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="22">
        <location>
            <position position="763"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="626"/>
            <end position="634"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23 25">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="73"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072209"/>
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        <source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50105"/>
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    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040973.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1003" mass="112307" checksum="1FB9C2FD974DB842" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLQ1</accession>
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        <recommendedName>
            <fullName evidence="12">ArfGAP with GTPase domain, ankyrin repeat and PH domain 2</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the centaurin gamma-like family.</text>
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        <property type="gene ID" value="ENSSORG00005019192.1"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003924">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="ARF-GAP WITH GTPASE, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 2"/>
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        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
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    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="9">
        <location>
            <begin position="73"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="33920" checksum="D89C1610C3D116F7" modified="2020-06-17" version="1">AERRANQKGDATIEEDNFDFLIVSSTGQTWHFEAQSVEERDSWVQAIESQILASLQLCESSKNKDRKNSQSEAVALQAIRNAKGNNFCVDCDAPNPTWVSLNLGALICIECSGIHRNLGTHLSRVRSLDLDDLPRELTLVLSAIGNHMVNSIWEARTMGHRKPSPDATREERESWIRAKYEQKLFVAPLPPPTPSEGPDLTLSGRLLLAVMEHNLPKLLLLLAHCTKEDINAPLSLALSSRALLYGCDLRYRDAQGQTALALARSAGSQECVDILLQYGCPNEPAPSVASTVGFAASVTSSFPVSVMLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNS6</accession>
    <name>A0A673BNS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycine N-acyltransferase-like protein</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">si:dkey-76k16.6</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673BNS6"/>
    <dbReference type="Ensembl" id="ENSSORT00005044014.1">
        <property type="protein sequence ID" value="ENSSORP00005042924.1"/>
        <property type="gene ID" value="ENSSORG00005019863.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BNS6"/>
    <dbReference type="OrthoDB" id="61870at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047961">
        <property type="term" value="F:glycine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010313">
        <property type="entry name" value="Glycine_N-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013652">
        <property type="entry name" value="Glycine_N-acyltransferase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015938">
        <property type="entry name" value="Glycine_N-acyltransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298:SF15">
        <property type="entry name" value="GLYCINE N-ACYLTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15298">
        <property type="entry name" value="L-COA N-ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08444">
        <property type="entry name" value="Gly_acyl_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06021">
        <property type="entry name" value="Gly_acyl_tr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="143"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="272" mass="30796" checksum="42D977B70AA6EE30" modified="2020-06-17" version="1">MELNEEQLKDAETKLKTYLPRSLTAYGTLVLMNRAKPDPMKVFVDTWPDFHVVVCRPEREQKGDSFKDIIVFATDETILKETIRNPCVIDWSAYLCVGVELQHSETFRAVASEKNICSSQLAFCHMMILNDVSGLPTTDSSVVSLSSLDESHIGLMNQTWKFGNDDAVPMIRNMIKNFPSCCVLDADGKPVSWVLTYASCAIGMLYTLPEHRGKGYAKVLISTMANRLHAQGLPVYCFIEKENTVSYNLFKSLGFTEDPSYKGTWFHFNYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSK5</accession>
    <name>A0A673BSK5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial</fullName>
            <ecNumber evidence="2">7.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase 24 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ndufv2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Parts of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone. Contains one iron-sulfur cluster.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:29092"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="16">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_030016424.1">
        <property type="nucleotide sequence ID" value="XM_030160564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BSK5"/>
    <dbReference type="FunCoup" id="A0A673BSK5">
        <property type="interactions" value="1533"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044661.1">
        <property type="protein sequence ID" value="ENSSORP00005043562.1"/>
        <property type="gene ID" value="ENSSORG00005020119.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437341"/>
    <dbReference type="CTD" id="4729"/>
    <dbReference type="InParanoid" id="A0A673BSK5"/>
    <dbReference type="OrthoDB" id="10254187at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000022">
        <property type="entry name" value="NADH dehydrogenase flavoprotein 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005722">
        <property type="entry name" value="PRK07539.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005725">
        <property type="entry name" value="PRK07539.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01257">
        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 16" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7 16" id="KW-0408">Iron</keyword>
    <keyword evidence="8 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="10" id="KW-0830">Ubiquinone</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="206"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="244" mass="26758" checksum="CABDBF4361FB336E" modified="2020-06-17" version="1">MFLSAAVRSAVSHTARQVRSLHRSAARAGAGGIFVHRDTPDNNPDTPFEFTEENKKRIEAIISMYPVGHKQAATIPVLDLAQRQHGWLPISAMNKVAEVLEVPPMRVYEVATFYTMFLRQPVGKYFIQICTTTPCMLCNSDSILEAIQNKLGIKVGETTPDKMFTLIEVECLGACVNAPMVQINDNYYEDLTPKDIEDIIDELKAGRVPPPGPRGGRFSCEPAGGLTSLTEPPKGPGFGVRADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BT05</accession>
    <name>A0A673BT05_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nfatc3a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BT05"/>
    <dbReference type="FunCoup" id="A0A673BT05">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045426.1">
        <property type="protein sequence ID" value="ENSSORP00005044304.1"/>
        <property type="gene ID" value="ENSSORG00005020423.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BT05"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01178">
        <property type="entry name" value="IPT_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07881">
        <property type="entry name" value="RHD-n_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF6">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="11">
        <location>
            <begin position="427"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="247"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="745"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="952"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1031"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="757"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="897"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="985"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1116" mass="120426" checksum="0C1B2E012059BA57" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVB1</accession>
    <name>A0A673BVB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">med7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BVB1"/>
    <dbReference type="FunCoup" id="A0A673BVB1">
        <property type="interactions" value="1295"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045620.1">
        <property type="protein sequence ID" value="ENSSORP00005044497.1"/>
        <property type="gene ID" value="ENSSORG00005020483.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVB1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051669">
        <property type="entry name" value="Immune_Mod/Transcr_Coactivator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15498:SF72">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15498">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN CONTAINING TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="227" mass="26177" checksum="77FADE9B44957A88" modified="2020-06-17" version="1">MGEPQQVSALPPPPMQYIKEYTDENIRKGLAPKPPPPIRDSYMMFGNQFQCDDLIIRPLESQGIERLHPVQFDHKRELKKLNMSILVNFLDLLDILIKSPGSLKRDEKLEDLKLLFVHMHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERVVEMIQSCLASLPDDLPQMDGPEEPMDAEEAGASCMAAGHQDRSTAGLKRDKIWDKDAAMCSIIDEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BW47</accession>
    <name>A0A673BW47_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115435358</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673BW47"/>
    <dbReference type="Ensembl" id="ENSSORT00005047216.1">
        <property type="protein sequence ID" value="ENSSORP00005046060.1"/>
        <property type="gene ID" value="ENSSORG00005021103.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BW47"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000011">
        <property type="entry name" value="Putative ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="294" mass="32034" checksum="E5CFA98B098E43FA" modified="2020-06-17" version="1">MPNIVLFSGSSHHDLSQKVADRLGLELGKVITKKFSNQETCVEIGESVRGEDVYIVQSGCGEINDNLMELLIMINACKIASSSRVTAVIPCFPYARQDKKDKSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIAVDNLYAEPAVLQWIRENIPEWKNCIIVSPDAGGAKRVTSIADRLNVEFALIHKERKKANEVDRMVLVGDVKDRVAIPGGTTWPTLAGTICHAADKLIDAGAIKVYAILTHGIFSGPAISRINNAPFEAVVVTNTIPQEEKMKACPKNTDYLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYW3</accession>
    <name>A0A673BYW3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cpeb2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPEB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987376.1">
        <property type="nucleotide sequence ID" value="XM_030131516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BYW3"/>
    <dbReference type="Ensembl" id="ENSSORT00005047027.1">
        <property type="protein sequence ID" value="ENSSORP00005045872.1"/>
        <property type="gene ID" value="ENSSORG00005021015.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417529"/>
    <dbReference type="CTD" id="132864"/>
    <dbReference type="InParanoid" id="A0A673BYW3"/>
    <dbReference type="OrthoDB" id="10033548at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008135">
        <property type="term" value="F:translation factor activity, RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000766">
        <property type="term" value="P:negative regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12724">
        <property type="entry name" value="RRM1_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12726">
        <property type="entry name" value="RRM2_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000008">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.40:FF:000001">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000009">
        <property type="entry name" value="cytoplasmic polyadenylation element-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032296">
        <property type="entry name" value="CEBP_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034819">
        <property type="entry name" value="CPEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT BINDING PROTEIN CPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566:SF8">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16366">
        <property type="entry name" value="CEBP_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16367">
        <property type="entry name" value="RRM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="490"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="598"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="190"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="298"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="747" mass="81894" checksum="8F7B54FAB9ED331E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZ10</accession>
    <name>A0A673BZ10_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pleckstrin and Sec7 domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">psd2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Ruffle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BZ10"/>
    <dbReference type="Ensembl" id="ENSSORT00005049416.1">
        <property type="protein sequence ID" value="ENSSORP00005048226.1"/>
        <property type="gene ID" value="ENSSORG00005021294.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BZ10"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13295">
        <property type="entry name" value="PH_EFA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000004">
        <property type="entry name" value="PH and SEC7 domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000054">
        <property type="entry name" value="PH and SEC7 domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF329">
        <property type="entry name" value="PH AND SEC7 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="9">
        <location>
            <begin position="234"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="454"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="38"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="692"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="710"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="723" mass="80433" checksum="7BEEBF3A8A279C43" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0U0</accession>
    <name>A0A673C0U0_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized LOC115410850</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115410850</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029978557.1">
        <property type="nucleotide sequence ID" value="XM_030122697.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSSORT00005050043.1">
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    </dbReference>
    <dbReference type="GeneID" id="115410850"/>
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    <dbReference type="InParanoid" id="A0A673C0U0"/>
    <dbReference type="OrthoDB" id="9045030at2759"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Connexin_N"/>
    </dbReference>
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        <property type="entry name" value="Connexin_N_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF20">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
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        <location>
            <begin position="14"/>
            <end position="228"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00029"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="237" mass="26946" checksum="46E9208D80D479E7" modified="2020-06-17" version="1">MAAIVTGLIPILRTAVDATTTYKCRSLWFGFLCIRVVILFLAELPFTKLDADFTCNGTRDSICSKLCFNEHFNKPMMVAWNFIFVLVVLSVLIMELFTSHLQSLAQKRNSQMRSDVNLETGGTEEAQVVSTDTRGKAFLDLHRDRGTVSVYLLSIALRVLAEAWFVYVLLFWNLPALNNNPYKCPTHICREFHVCVVRAAPEKRMSIYALASISGMIIVCSLLFWIYSIAHYLCNFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C279</accession>
    <name>A0A673C279_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C279"/>
    <dbReference type="Ensembl" id="ENSSORT00005050577.1">
        <property type="protein sequence ID" value="ENSSORP00005049376.1"/>
        <property type="gene ID" value="ENSSORG00005022430.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C279"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="120" mass="13543" checksum="99C5100D88460BF2" modified="2020-06-17" version="1">MSFTAVTGQIYFFTVRDGDDVTLPCTGVIPDQDQCSQTWRFTNPPGEMTEFVFSRRVGHPGKKLDRQSVSANCSLVIKKVTVNDAGRYVCMQRDVSGSKHEVILSVVTSEYLHQNIWILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3B5</accession>
    <name>A0A673C3B5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mkxa</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C3B5"/>
    <dbReference type="Ensembl" id="ENSSORT00005047974.1">
        <property type="protein sequence ID" value="ENSSORP00005046808.1"/>
        <property type="gene ID" value="ENSSORG00005021419.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C3B5"/>
    <dbReference type="OrthoDB" id="21495at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN MOHAWK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="65"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="67"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="363" mass="40476" checksum="485E6A49FF36042D" modified="2020-06-17" version="1">MNTIVFNKLSSQVLFEEKAKEVEMSSRNYLEVIDGQHPDLLSSNQTIRDNQAIRHRRSGGRPSGGKVRHKRQALQDMARPLKQWLYKHRDNPYPTKTEKILLALGSQMTLVQVSNWFANARRRLKNTVRQPDLSWALRIKLYNKYVQGNAERLSVSSDDSCSEDGDNPQRTHSGPEELNKPMYQSVIKKEGSSMVGMAMGMGAGLRSAAEAASLTEDYVSPPKYKSSLLHRYLNDSLRHVMVANAVMDARKRNHSGSFSSNEYDDDLLSPSSSEAEANFVYRAETTDHGSSKCDIGSSGISVGQKEKVKGKDETYWKEINAAMALTNLAQGKDSASGTTSCIIQKSSHIAEVKTVKVPLVQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C741</accession>
    <name>A0A673C741_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glypican-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC115417728</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Cell membrane</location>
            <topology evidence="2 16">Lipid-anchor</topology>
            <topology evidence="2 16">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C741"/>
    <dbReference type="FunCoup" id="A0A673C741">
        <property type="interactions" value="776"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051008.1">
        <property type="protein sequence ID" value="ENSSORP00005049805.1"/>
        <property type="gene ID" value="ENSSORG00005022594.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C741"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040037">
        <property type="term" value="P:negative regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905475">
        <property type="term" value="P:regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001863">
        <property type="entry name" value="Glypican"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019803">
        <property type="entry name" value="Glypican_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822:SF8">
        <property type="entry name" value="GLYPICAN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01153">
        <property type="entry name" value="Glypican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01207">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="13 16" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="14 16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025616295" description="Glypican-1" evidence="18">
        <location>
            <begin position="23"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="542" mass="59618" checksum="2D0E5FBD04E5B714" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9J6</accession>
    <name>A0A673C9J6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neoverrucotoxin subunit alpha-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115410667</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNTX/VTX toxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C9J6"/>
    <dbReference type="Ensembl" id="ENSSORT00005053504.1">
        <property type="protein sequence ID" value="ENSSORP00005052259.1"/>
        <property type="gene ID" value="ENSSORG00005023564.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053521.1">
        <property type="protein sequence ID" value="ENSSORP00005052276.1"/>
        <property type="gene ID" value="ENSSORG00005023564.1"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="SI:CH211-281L24.3"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="700" mass="79213" checksum="C9F709081C85CAB1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CA39</accession>
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        <name evidence="13" type="primary">pak2b</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Apogonidae</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="77"/>
            <end position="90"/>
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    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
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            <end position="508"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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        </location>
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    <feature type="binding site" evidence="9">
        <location>
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        <ligand>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="532" mass="58649" checksum="BAC90D071A50A68F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CEH2</accession>
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        <submittedName>
            <fullName evidence="5">Astrotactin 1</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007158">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR026995">
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    <dbReference type="PANTHER" id="PTHR16592">
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        <property type="entry name" value="ASTN_1_2_N"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
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            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025432082" evidence="3">
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            <end position="472"/>
        </location>
    </feature>
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        <location>
            <begin position="161"/>
            <end position="185"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Astrotactin-1/2 N-terminal" evidence="4">
        <location>
            <begin position="115"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="472" mass="52759" checksum="36CAB01355C0AB3F" modified="2020-06-17" version="1">MASINMGLVPLLVPLLVRVAWVLLVLRTAHVHGLNGDEDQNQDTECKMKSVTVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGDVVVVDDLENTELPFFVLGESGPLSTTSPLVRWRQQWLENGTLLFHIHHQDGSLSLPEPTEDPANDSAKEELRILHISVMGGMIALLLSILCLVLILYTRRRWCKRRRIPQPQKSASAEAANEIHYIPSVLMGGQARESLRNSRGQGPNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEEFASQVTRTLDSLQGCNDKNNMDLAPGSDNTKLALMNKYKDNIIATSPIDSNHQQNTLLPHNTSTSQRKRLSSNTRGSPRSPINKTTLTLISVISCVIGLVYSSHLACSLSVRVILHVPEHLIADGSTAGSMHWLLLAYWHFSAVMGVRWQSRYIIDCDEAHSSIIWRSDLIRSVASFRWHTLACQEDIKIPVRRPGREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGE6</accession>
    <name>A0A673CGE6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Germ cell-specific gene 1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115427259</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CGE6"/>
    <dbReference type="FunCoup" id="A0A673CGE6">
        <property type="interactions" value="1212"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052382.1">
        <property type="protein sequence ID" value="ENSSORP00005051158.1"/>
        <property type="gene ID" value="ENSSORG00005023123.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGE6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032279">
        <property type="term" value="C:asymmetric synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000005">
        <property type="entry name" value="Germ cell-specific gene 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012478">
        <property type="entry name" value="GSG-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF35">
        <property type="entry name" value="GERM CELL-SPECIFIC GENE 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07803">
        <property type="entry name" value="GSG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="284" mass="32494" checksum="1A2FD7BC4A1D11E1" modified="2020-06-17" version="1">MKTTRKCRTLLSLSLNFFALFFSITAFITTYWCVGTQRVPKPKCSKLRTHQCIDYGVNETDPNKVVYSWETGDDRFLFRQFHTGIWFSCEENIHDESERCRSFIDLAPASEKGMLWLSLVSEMLYIILLVVGFSLMCLELVHSSNVIDGLKLNAFAAVFTVLSGLLGMVAHVMYTQVFQVTVSHGPPEWRPYNWDYGWSFCMAWASFTFCMGASVTTLNSYTKTVIEFRHKRGFGNFRDRSVHSISKSVEVYTSQSLKSGRRTPIPADSLDLNDIHRSLGEEQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKT1</accession>
    <name>A0A673CKT1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115433703</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_030011022.1">
        <property type="nucleotide sequence ID" value="XM_030155162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CKT1"/>
    <dbReference type="Ensembl" id="ENSSORT00005057516.1">
        <property type="protein sequence ID" value="ENSSORP00005056221.1"/>
        <property type="gene ID" value="ENSSORG00005025023.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433703"/>
    <dbReference type="InParanoid" id="A0A673CKT1"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000101">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="148" mass="16609" checksum="0FE6B572ED0EA441" modified="2020-06-17" version="1">MALKRLQKELGELQRDPPARCSAGPVGDDMFSWQATIMGPSDSPYNGGVFFLNINFPTDYPFKPPKVSFKTKVYHPNINSEGGGICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAYLYKSDRQKYNTMAKEWTQKYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKY9</accession>
    <name>A0A673CKY9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calmodulin regulated spectrin-associated protein 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">camsap1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A673CKY9">
        <property type="interactions" value="1161"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055224.1">
        <property type="protein sequence ID" value="ENSSORP00005053954.1"/>
        <property type="gene ID" value="ENSSORG00005024221.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKY9"/>
    <dbReference type="Proteomes" id="UP000472271">
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    <sequence length="1516" mass="168350" checksum="BC305738DC029A7A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CP82</accession>
    <name>A0A673CP82_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoprotein endo-alpha-1,2-mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.130</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">manea</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-{alpha-Glc-(1-&gt;3)-alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;3)-[alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;3)-[alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;6)]-alpha-Man-(1-&gt;6)]-beta-Man-(1-&gt;4)-beta-GlcNAc-(1-&gt;4)-beta-GlcNAc}-L-asparaginyl-[protein] + H2O = alpha-D-glucosyl-(1-&gt;3)-D-mannopyranose + N(4)-{alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlaNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,2)</text>
            <dbReference type="Rhea" id="RHEA:54824"/>
            <dbReference type="Rhea" id="RHEA-COMP:14010"/>
            <dbReference type="Rhea" id="RHEA-COMP:14011"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:52996"/>
            <dbReference type="ChEBI" id="CHEBI:59080"/>
            <dbReference type="ChEBI" id="CHEBI:60627"/>
            <dbReference type="EC" id="3.2.1.130"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 99 family.</text>
    </comment>
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        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056422.1">
        <property type="protein sequence ID" value="ENSSORP00005055144.1"/>
        <property type="gene ID" value="ENSSORG00005024642.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:glycoprotein endo-alpha-1,2-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="entry name" value="GH99"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosidases"/>
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        <property type="entry name" value="Glyco_Hydrolase_99"/>
    </dbReference>
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        <property type="entry name" value="ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572:SF1">
        <property type="entry name" value="GLYCOPROTEIN ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_hydro_99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
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            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00049330"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
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        </source>
    </evidence>
    <sequence length="446" mass="51554" checksum="413910C16F2E780A" modified="2020-06-17" version="1">MARFKRKSCVALFALVLFVLVVTVVLKFLTPEDSPLSSPLGGELFADRKNDNQIQKDNKADGKPKQTKMSNSPQPENRAELEATLRKFPPPNYYLHAFYYPWYGNPKFDGKYIHWDHPQLPHWDYKVAQGYPQGRHSPPDDIGSNFYPALGPYSSRDPAVMEAHMQQLRTAAIGVLVVSWYPPNTKDDNGEPVDNIVPLLLEVAHKYHIKVAFHIEPYKGRDEVNMFTNVKYIIEKYGEHPAFFKYRTNTGKLLPLFYVYDSYLMNSDQWAKLLKHTESNSIRDTPYDAIFIALLVEEKHKRDILTAGFDGFYTYFATNGFSYGSTQKNWDSIKAFCEDNNLIFIPSVGPGYIDTSIRPWNFQNTRNRINGKYYETSLSAALEARPDFISITSFNEWHEGTQIEMAVPKTSQTVYLDYLPNKPSIYLEITRKWAAIFGSERRRRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQF1</accession>
    <name>A0A673CQF1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Lamin B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lmnb1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus lamina</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030005527.1">
        <property type="nucleotide sequence ID" value="XM_030149667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CQF1"/>
    <dbReference type="Ensembl" id="ENSSORT00005056083.1">
        <property type="protein sequence ID" value="ENSSORP00005054807.1"/>
        <property type="gene ID" value="ENSSORG00005024520.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429870"/>
    <dbReference type="CTD" id="4001"/>
    <dbReference type="InParanoid" id="A0A673CQF1"/>
    <dbReference type="OrthoDB" id="102442at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005652">
        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090435">
        <property type="term" value="P:protein localization to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1260">
        <property type="entry name" value="Lamin Tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036415">
        <property type="entry name" value="Lamin_tail_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721">
        <property type="entry name" value="LAMIN DM0-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45721:SF3">
        <property type="entry name" value="LAMIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74853">
        <property type="entry name" value="Lamin A/C globular tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="13">
        <location>
            <begin position="31"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="12">
        <location>
            <begin position="430"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="384"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="127"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="285"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="65816" checksum="0AED1B110A1F6108" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSS9</accession>
    <name>A0A673CSS9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lon protease homolog, mitochondrial</fullName>
            <ecNumber evidence="11">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">lonp1</name>
        <name evidence="11" type="synonym">LONP1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer or homoheptamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="11"/>
    <dbReference type="RefSeq" id="XP_029988075.1">
        <property type="nucleotide sequence ID" value="XM_030132215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CSS9"/>
    <dbReference type="FunCoup" id="A0A673CSS9">
        <property type="interactions" value="1458"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056887.1">
        <property type="protein sequence ID" value="ENSSORP00005055602.1"/>
        <property type="gene ID" value="ENSSORG00005024811.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417967"/>
    <dbReference type="CTD" id="9361"/>
    <dbReference type="InParanoid" id="A0A673CSS9"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070407">
        <property type="term" value="P:oxidation-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000043">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000001">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1480:FF:000002">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.40:FF:000004">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000015">
        <property type="entry name" value="Lon protease homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03120">
        <property type="entry name" value="lonm_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027503">
        <property type="entry name" value="Lonm_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
            <begin position="164"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="15">
        <location>
            <begin position="810"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="260"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="905"/>
        </location>
    </feature>
    <feature type="active site" evidence="11 12">
        <location>
            <position position="948"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="574"/>
            <end position="581"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
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        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055602.1"/>
        </source>
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    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1000" mass="111441" checksum="68936A30C839B40B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CUC2</accession>
    <name>A0A673CUC2_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">Resistance to inhibitors of cholinesterase protein 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CUC2"/>
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        <property type="gene ID" value="ENSSORG00005025643.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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    </dbReference>
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        <property type="term" value="C:neuronal cell body"/>
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    </dbReference>
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        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="P:protein localization to cell surface"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:synaptic transmission, cholinergic"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN RIC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723">
        <property type="entry name" value="RESISTANCE TO INHIBITORS OF CHOLINESTERASE PROTEIN 3 RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025437100" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="30"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal" evidence="3">
        <location>
            <begin position="16"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="127" mass="14326" checksum="F1C5657731216D7E" modified="2020-06-17" version="1">MSITTCQKVTLISCSVLCVSLFLPRMLLPRGKEDMGQPEVGPGFYPPVMHQLSFPEDSDQWGVDPSYSMTHNAEIVAKVKGIGRKKYNLMAQVIPIYGFGILLYIFYIIYKVTVFYLYVFTSIALSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CY13</accession>
    <name>A0A673CY13_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Cadherin-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">cdh2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 19">Cell membrane</location>
            <topology evidence="3 19">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CY13"/>
    <dbReference type="FunCoup" id="A0A673CY13">
        <property type="interactions" value="542"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059041.1">
        <property type="protein sequence ID" value="ENSSORP00005057722.1"/>
        <property type="gene ID" value="ENSSORG00005025345.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CY13"/>
    <dbReference type="OrthoDB" id="6079678at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099634">
        <property type="term" value="C:postsynaptic specialization membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048787">
        <property type="term" value="C:presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell migration involved in gastrulation"/>
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        <property type="entry name" value="Cadherin-like_sf"/>
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    <keyword evidence="12 18 19" id="KW-0106">Calcium</keyword>
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    <keyword evidence="14" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8 19 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
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            <begin position="719"/>
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            <begin position="608"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="910" mass="100363" checksum="D66785DA3B496C3B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CZ08</accession>
    <name>A0A673CZ08_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Synaptotagmin-7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Synaptotagmin VII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">syt7b</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="24">Homodimer. Can also form heterodimers with SYT6, SYT9 and SYT10. Interacts with calmodulin (CALM1, CALM2 or CALM3). Interacts with CD63; required for localization to lysosomes. Interacts with APP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Cytoplasmic vesicle</location>
            <location evidence="23">Phagosome membrane</location>
            <topology evidence="23">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Presynaptic cell membrane</location>
            <topology evidence="22">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the synaptotagmin family.</text>
    </comment>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:positive regulation of transport"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050796">
        <property type="term" value="P:regulation of insulin secretion"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:vesicle fusion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="C2 domain"/>
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    <accession>A0A672Y709</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="203" mass="23540" checksum="FB46AD4C2BF5C266" modified="2020-06-17" version="1">MWWVLWVLAALLALFCCLDVWYFLRVPLVIIQAWFQPPVWDITAEQTLTGRVTLHDIDMCHMNNARYLRECDFARFSLYTRNGVFKAARALGASMVVGATTIRYRRALCIGEGYELRSRIVTWDDKAFYLEQRFVSTKDGLVCAVMYCKQSVVRGSPDKIMQHLCKRKVECPEFPEDLQHWVNFISASSQALRAESGLEEKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y912</accession>
    <name>A0A672Y912_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Immunoglobulin lambda-1 light chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115424018</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y912"/>
    <dbReference type="Ensembl" id="ENSSORT00005003392.1">
        <property type="protein sequence ID" value="ENSSORP00005003293.1"/>
        <property type="gene ID" value="ENSSORG00005002008.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y912"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07699">
        <property type="entry name" value="IgC1_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000283">
        <property type="entry name" value="Immunoglobulin kappa constant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256:SF65">
        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025518082" evidence="8">
        <location>
            <begin position="18"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="19"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="139"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="241" mass="26032" checksum="BA79928E780FDD2A" modified="2020-06-17" version="1">MLVTLCTLITALTYADAVKVLTQTPAVHTVSPGQEVVLKCNVQTDYDYVSWYKQVPGEAPQFVLRFYHSHSSPSYGTGFSSDRFNSKTTSNIDYQLIIKNAEAGDSAQYFCGGWNNSPRVAVFGPGTKLTVTSSSVPPPVLTVFPPSAAELQSNKATLVCLSSQSVAFAHVTWLSAGSPLSTGISTSTAVQQPDQTFQISSYLSIQTSDWNTDQVYKCKVSVGSHTSEKHINKSACPTEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9F2</accession>
    <name>A0A672Y9F2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein tweety homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Probable chloride channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the tweety family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y9F2"/>
    <dbReference type="Ensembl" id="ENSSORT00005003441.1">
        <property type="protein sequence ID" value="ENSSORP00005003342.1"/>
        <property type="gene ID" value="ENSSORG00005002038.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y9F2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072320">
        <property type="term" value="F:volume-sensitive chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF17">
        <property type="entry name" value="PROTEIN TWEETY HOMOLOG 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04906">
        <property type="entry name" value="Tweety"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 13" id="KW-0868">Chloride</keyword>
    <keyword evidence="9 13" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="513" mass="57589" checksum="D60F2A8FD07DA5F4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAB1</accession>
    <name>A0A672YAB1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA-binding protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115435001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030013030.1">
        <property type="nucleotide sequence ID" value="XM_030157170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YAB1"/>
    <dbReference type="Ensembl" id="ENSSORT00005001173.1">
        <property type="protein sequence ID" value="ENSSORP00005001139.1"/>
        <property type="gene ID" value="ENSSORG00005000684.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435001"/>
    <dbReference type="InParanoid" id="A0A672YAB1"/>
    <dbReference type="OrthoDB" id="346910at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="86" mass="9694" checksum="A046817097D7FD1C" modified="2020-06-17" version="1">MTESFQKAAEEAKVLLQKPDKDELSALYGLYKQATVGDVNIDRPGILDFTGRGKWDAWNARKGLSQDEAMVSYIELVEKLKIKYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAJ1</accession>
    <name>A0A672YAJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kynurenine formamidase</fullName>
            <shortName evidence="5">KFA</shortName>
            <shortName evidence="5">KFase</shortName>
            <ecNumber evidence="5">3.5.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arylformamidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">N-formylkynurenine formamidase</fullName>
            <shortName evidence="5">FKF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">afmid</name>
        <name evidence="5" type="synonym">AFMID</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-formyl-L-kynurenine + H2O = L-kynurenine + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13009"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="3.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the kynurenine formamidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.9" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672YAJ1"/>
    <dbReference type="FunCoup" id="A0A672YAJ1">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000068.1">
        <property type="protein sequence ID" value="ENSSORP00005000065.1"/>
        <property type="gene ID" value="ENSSORG00005000042.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAJ1"/>
    <dbReference type="UniPathway" id="UPA00333">
        <property type="reaction ID" value="UER00454"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000134">
        <property type="entry name" value="Kynurenine formamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03014">
        <property type="entry name" value="KFase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027519">
        <property type="entry name" value="KFase_ver/fungi-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="6">
        <location>
            <begin position="80"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HGGXW" evidence="5">
        <location>
            <begin position="84"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="299" mass="33563" checksum="2FF90EAF60016CD0" modified="2020-06-17" version="1">MTHWTKMSKDELERQYSPSRWSHRMSADDVIKAHVKALKEGTERARGLAQTLLNVPYGQEDGEKLDVYIPRTNSLDMPLVIYLHGGYWQFLSKDESGFMAVPLIDKGVVVVAVGYDIAPKGNMDLMVSQVRRSVVSVIQQYSHISGLYICGHSAGAHLAAMVLSTDWSQYSIAPQIKGAFLISGIYDLLPILSTYVNEPLKMTEEVALRNSPSQLVPQLKLSSSGCHVVVAVAENDSPEFRKQSEEYYKTLQESGLNVTLEDVPNTDHFSVVELLVDGEYHLTKHKSLIYYQLLFMYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YC18</accession>
    <name>A0A672YC18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pseudouridylate synthase RPUSD4, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">RNA pseudouridylate synthase domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115418847</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YC18"/>
    <dbReference type="SMR" id="A0A672YC18"/>
    <dbReference type="FunCoup" id="A0A672YC18">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000162.1">
        <property type="protein sequence ID" value="ENSSORP00005000146.1"/>
        <property type="gene ID" value="ENSSORG00005000118.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YC18"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000015">
        <property type="entry name" value="Mitochondrial RNA pseudouridine synthase RPUSD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF83">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="11">
        <location>
            <begin position="128"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="391" mass="43750" checksum="1208CFB3EF9517C5" modified="2020-06-17" version="1">LATLKLLNRLSVIFIDRGPGLNVIISRVKTHTKCRLCPQGSLPFSQFEAAGTRHAPDAGTEDKPRLRAIDLARKIKQQRTETRAQAPPVSAQQKRVMELKRFSQQLQNVHPNVLAKHLRRSVLYQDKDVVIINKPYGVPVRDDSGATSISSVLPVLSKLMDGVKVKSDSQLLPCLSLDKETTGALLLARTEEALEKILNQHRNNQVRRTYWVITVGVPVPSEGLIDIPIIEREVTGPQPHFKMALSPLFRMNDTGDSVIKVRAHRQAQSAATKYRVLDSSSGCSLVELQPLSGVKHQMRVHLAFALACPILGDHKYSHWSKLAPQKLPERVLGRLGLEQSKIRYLPLHLHARQLTLPGTSQNDINVSSPLPKYFSQTLSQLHLKFPDEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCI7</accession>
    <name>A0A672YCI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YCI7"/>
    <dbReference type="Ensembl" id="ENSSORT00005002764.1">
        <property type="protein sequence ID" value="ENSSORP00005002684.1"/>
        <property type="gene ID" value="ENSSORG00005001652.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCI7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF847">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="36320" checksum="1DE53430E07AC9DB" modified="2020-06-17" version="1">MGNNSYAKYITITGFVELDKYRYVYFSIVFTVYVLIISSNSIIIYLILIHKNLHEPMYVFIAALSFNSVVFSTTVYPKLLIDFLSVQPVISVSACIFQNFIYYSSGGSDFLLLLAMAFDRYVSICKPLQYPTIMRKTTVCLFLLGAWLVPDAQVAAGNFLLISQNPCIFTMTGIFCNNSYLILFCDSSNMLALYGVFILLNVVLLPVIFILFTYAKILTITYNSGREHRKKAAETCLPHLLVLFSFSFLCIYYVVIGIFQSVFSKTLLLIMNLQVVLYNPLLNPVIYGLKMTEISKHLKILFFHEKVFILREYTVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCM2</accession>
    <name>A0A672YCM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YCM2"/>
    <dbReference type="Ensembl" id="ENSSORT00005001453.1">
        <property type="protein sequence ID" value="ENSSORP00005001410.1"/>
        <property type="gene ID" value="ENSSORG00005000853.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCM2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000013">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF15">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="12"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="114"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="310" mass="33305" checksum="8C7AE9613E1D0452" modified="2020-06-17" version="1">MSVCCCGSTDSVRLVHGSSLCSGRLEVRSNQSWFSVCEEDLDLNDTQVVCRELGCGAPGLLQGALYGEGEAPVWTSELQCEGNESAVLDCRRSSSAGKTCSPGTAVGLTCSGGVRMVGQPSHCAGTLEIQHQGQWRPVQDYDGDWDLKSGSAVCQYLDCGSAVSVNRTDDSADRRVWLVSVPCVKLTSGLRDCVGLYDSYTDSSGVEVVCSDLLPQPNISLSDGVFGVYQQGFWVLVDSDFTITCSVRPQYPGGSFQLISDTKKPLNLTLPAVNHSAHFLLSSMGYAHRGNYTCVYHVDVYNHSFSSSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCY8</accession>
    <name>A0A672YCY8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">AE binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">aebp2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the AEBP2/jing C2H2-type zinc-finger family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YCY8"/>
    <dbReference type="Ensembl" id="ENSSORT00005001678.1">
        <property type="protein sequence ID" value="ENSSORP00005001630.1"/>
        <property type="gene ID" value="ENSSORG00005001019.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YCY8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000471">
        <property type="entry name" value="Zinc finger protein AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052130">
        <property type="entry name" value="AEBP2/jing_C2H2-ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059034">
        <property type="entry name" value="SH3_AEBP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46541">
        <property type="entry name" value="ZINC FINGER PROTEIN AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46541:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN AEBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26014">
        <property type="entry name" value="SH3_AEBP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="254" mass="28204" checksum="A1A067A8735A9F23" modified="2020-06-17" version="1">MDIESAASSGRSTPAMLNGHSGGAGAGSGSAPLNGKSLSYTCCWDQCQLQFPSSPDLAEHIRATHVDGQRGGVFVCLWKGCKVYNTPSTSQSWLQRHMLTHSGDKPFKLSEFIQSVQSGQVKEESPSKAGLNKRRKIKNKHRRSLPRPHDFFDAQTMDAIRHRAICLNLATHIESLGNGHSVVFHSTVIARRKEDSGKVKVLLHWTPEDILPDVWVNESERLQQKTKVVHLSKLPTDTAVLLDPNIYRFMSMLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFE3</accession>
    <name>A0A672YFE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-N-acetylglucosaminidase</fullName>
            <ecNumber evidence="7">3.2.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">naglu</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides.</text>
            <dbReference type="EC" id="3.2.1.50"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 89 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.50" evidence="7"/>
    <dbReference type="RefSeq" id="XP_029977805.1">
        <property type="nucleotide sequence ID" value="XM_030121945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YFE3"/>
    <dbReference type="FunCoup" id="A0A672YFE3">
        <property type="interactions" value="350"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002657.1">
        <property type="protein sequence ID" value="ENSSORP00005002591.1"/>
        <property type="gene ID" value="ENSSORG00005001563.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410337"/>
    <dbReference type="CTD" id="4669"/>
    <dbReference type="InParanoid" id="A0A672YFE3"/>
    <dbReference type="OrthoDB" id="64736at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004561">
        <property type="term" value="F:alpha-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000107">
        <property type="entry name" value="Alpha-N-acetylglucosaminidase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.670">
        <property type="entry name" value="N-acetyl-b-d-glucoasminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007781">
        <property type="entry name" value="NAGLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024732">
        <property type="entry name" value="NAGLU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024240">
        <property type="entry name" value="NAGLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024733">
        <property type="entry name" value="NAGLU_tim-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12872">
        <property type="entry name" value="ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12872:SF1">
        <property type="entry name" value="ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05089">
        <property type="entry name" value="NAGLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12972">
        <property type="entry name" value="NAGLU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12971">
        <property type="entry name" value="NAGLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025615742" description="Alpha-N-acetylglucosaminidase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase N-terminal" evidence="11">
        <location>
            <begin position="43"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase tim-barrel" evidence="10">
        <location>
            <begin position="140"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-N-acetylglucosaminidase C-terminal" evidence="12">
        <location>
            <begin position="483"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="751" mass="86104" checksum="A87650D1BAFADAC7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGG2</accession>
    <name>A0A672YGG2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TSC22 domain family protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115418532</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TSC-22/Dip/Bun family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029988896.1">
        <property type="nucleotide sequence ID" value="XM_030133036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YGG2"/>
    <dbReference type="FunCoup" id="A0A672YGG2">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003748.1">
        <property type="protein sequence ID" value="ENSSORP00005003642.1"/>
        <property type="gene ID" value="ENSSORG00005002193.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418532"/>
    <dbReference type="InParanoid" id="A0A672YGG2"/>
    <dbReference type="OrthoDB" id="8961796at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21941">
        <property type="entry name" value="ZIP_TSC22D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000002">
        <property type="entry name" value="TSC22 domain family, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQB9</accession>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YQB9"/>
    <dbReference type="FunCoup" id="A0A672YQB9">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007053.1">
        <property type="protein sequence ID" value="ENSSORP00005006791.1"/>
        <property type="gene ID" value="ENSSORG00005003949.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YQB9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029360">
        <property type="entry name" value="SRRM_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15230">
        <property type="entry name" value="SRRM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/arginine repetitive matrix protein C-terminal" evidence="2">
        <location>
            <begin position="287"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="40648" checksum="2BA0966C7D8908EF" modified="2020-06-17" version="1">EAGQLEEKQLFEKFWKGTFKAVATPRPESVIVASITARRRFPLCFIFPRSPSFDEELSPRPKGKKKKRKSERKRKRKRSVLKRRWQDSACLVLSYSVFVCLQILSSKPVSELLQEKESHHLSSQTEAPHDYDSGNDTSSPPSSKTGVSRASVTEIKRNHCQRLATDRDNASDSGNSVTSYASLCKPYREESLSVVRSPKTSSCPQRLSRASRRRLKNRTSSSSRSRSRSSGSSRYSGRYSRSPTLSSSIGKVYRVLSNGALKQIHIYMHIYVVAIKVSHCYVCFLRLRDRKRTPSSRESDAKHEHKVSGKRRRRKSYSPMKKRRRDSPSHLEARRITRCYRPHRPSPQLETSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTG3</accession>
    <name>A0A672YTG3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Family with sequence similarity 241 member A</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM241 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YTG3"/>
    <dbReference type="FunCoup" id="A0A672YTG3">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008123.1">
        <property type="protein sequence ID" value="ENSSORP00005007840.1"/>
        <property type="gene ID" value="ENSSORG00005004422.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTG3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027953">
        <property type="entry name" value="DUF4605"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052502">
        <property type="entry name" value="FAM241_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33690">
        <property type="entry name" value="DUF4605 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33690:SF1">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 241 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15378">
        <property type="entry name" value="DUF4605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4605" evidence="8">
        <location>
            <begin position="49"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="108" mass="12161" checksum="644C490D67CF9551" modified="2020-06-17" version="1">NITIKYNTELVVPGTTNPPRAPGGTGVHRAHRDHPPRQPQLDDCERMGTLFGMLNKCLRGMGFSQMYFGDKTVEPVVMVFFWLLLWFLGIQALGLVGTLCIIIIYIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTV7</accession>
    <name>A0A672YTV7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">aspm</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YTV7"/>
    <dbReference type="Ensembl" id="ENSSORT00005008296.1">
        <property type="protein sequence ID" value="ENSSORP00005008013.1"/>
        <property type="gene ID" value="ENSSORG00005004486.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YTV7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051295">
        <property type="term" value="P:establishment of meiotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21223">
        <property type="entry name" value="CH_ASPM_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21224">
        <property type="entry name" value="CH_ASPM_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000051">
        <property type="entry name" value="Abnormal spindle-like microcephaly-associated protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000008">
        <property type="entry name" value="Abnormal spindle-like microcephaly-associated protein homolog"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051185">
        <property type="entry name" value="ASPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22706:SF1">
        <property type="entry name" value="ASSEMBLY FACTOR FOR SPINDLE MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="170"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="12">
        <location>
            <begin position="355"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1140" mass="131955" checksum="A6328D180FD1D253" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWE4</accession>
    <name>A0A672YWE4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cerebral dopamine neurotrophic factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdnf</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARMET family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YWE4"/>
    <dbReference type="Ensembl" id="ENSSORT00005009220.1">
        <property type="protein sequence ID" value="ENSSORP00005008918.1"/>
        <property type="gene ID" value="ENSSORG00005004916.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YWE4"/>
    <dbReference type="OrthoDB" id="5597848at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071542">
        <property type="term" value="P:dopaminergic neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000003">
        <property type="entry name" value="Mesencephalic astrocyte-derived neurotrophic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045333">
        <property type="entry name" value="ARMET-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019345">
        <property type="entry name" value="ARMET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045332">
        <property type="entry name" value="ARMET_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12990">
        <property type="entry name" value="ARMET-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12990:SF9">
        <property type="entry name" value="CEREBRAL DOPAMINE NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10208">
        <property type="entry name" value="ARMET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20145">
        <property type="entry name" value="ARMET_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025529512" evidence="6">
        <location>
            <begin position="21"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="ARMET N-terminal" evidence="8">
        <location>
            <begin position="22"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="ARMET C-terminal" evidence="7">
        <location>
            <begin position="126"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="171" mass="19027" checksum="41202714163744A3" modified="2020-06-17" version="1">MLVFSLVILLFFRRASPSAATNCEVCVGFLQKVYGSVTSAHRDLTPALVEDELSRTCAAAQGKEARLCYYLGASSDAATRVTSSVSQPLSSHVPVEKVCQRLSSRDTQICELRYDPQLRDLSSDVLRKMRVVELRNILASWGEECRGCLEKHEFVSLIQEKAPLHVHSVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXC3</accession>
    <name>A0A672YXC3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rho guanine nucleotide exchange factor (GEF) 39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">arhgef39</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YXC3"/>
    <dbReference type="FunCoup" id="A0A672YXC3">
        <property type="interactions" value="875"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009569.1">
        <property type="protein sequence ID" value="ENSSORP00005009263.1"/>
        <property type="gene ID" value="ENSSORG00005005073.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YXC3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042987">
        <property type="entry name" value="ARHGEF39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47056">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47056:SF1">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="26"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="231"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="364" mass="42085" checksum="04731F60D48E25E5" modified="2020-06-17" version="1">MSFSPGPSGLHGSNSIQEQRDRWERKRSRTAKELVQTEQRYCQQLQLLTTYFVEILKAKGTLRQDIRESIFSSIKAIHSVNQSLLVHLENGYFGRGFDQFCPYLHHYNMYADNIYNASKVLTIQVRKNKAFRRFKKLQEARPEFNGHKLEDLLQLPIQRIDQYKHFLQDLTANTSPDNPEFQQLSRAVSAVSEVSQRIENNTRRHENHLQLCRVQKLLKGRKTKVLAPGRWYIREGWLKMVPAKGAEAKPKMFFLFSDMLLQAKRCSPLHPTNGDKFEGQHAYPLQDCTVEKVFGHTRSQGGLLSLSFPKAKLLLMSSNQENLNDWYQTLSSAIGYGTTHTHTHTHTHTHTRLHQIALSGHPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXY7</accession>
    <name>A0A672YXY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Zinc finger and BTB domain containing 16a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">zbtb16a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YXY7"/>
    <dbReference type="FunCoup" id="A0A672YXY7">
        <property type="interactions" value="724"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009890.1">
        <property type="protein sequence ID" value="ENSSORP00005009571.1"/>
        <property type="gene ID" value="ENSSORG00005005228.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YXY7"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18205">
        <property type="entry name" value="BTB_POZ_ZBTB16_PLZF"/>
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        <property type="entry name" value="Zinc finger and BTB domain-containing protein 16"/>
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        <property type="entry name" value="Zinc finger and BTB domain-containing protein 16"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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        <property type="entry name" value="AGAP004733-PA"/>
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        <property type="entry name" value="zf-C2H2"/>
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    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
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    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="16">
        <location>
            <begin position="34"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
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            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="422"/>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="451"/>
            <end position="478"/>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="480"/>
            <end position="507"/>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="508"/>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="537"/>
            <end position="564"/>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="565"/>
            <end position="592"/>
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        <location>
            <begin position="593"/>
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    </feature>
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            <begin position="130"/>
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    </feature>
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        <location>
            <begin position="242"/>
            <end position="283"/>
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    </feature>
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        <location>
            <begin position="182"/>
            <end position="193"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009571.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="664" mass="74109" checksum="D6504A081CF3A9D3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z3U1</accession>
    <name>A0A672Z3U1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Coagulation factor VIII-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">f8</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multicopper oxidase family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:signaling receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ceruloplasmin"/>
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    </dbReference>
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        <property type="entry name" value="Cu-oxidase-like_N"/>
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        <property type="entry name" value="Cu_oxidase_CS"/>
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        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="3" id="KW-0964">Secreted</keyword>
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        <location>
            <begin position="1362"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="12">
        <location>
            <begin position="1515"/>
            <end position="1669"/>
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    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="788"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="831"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="858"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="877"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="288"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="560"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="661"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1173"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1240"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1362"/>
            <end position="1510"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000354-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1675" mass="189287" checksum="A8B89CE24210DB8F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z501</accession>
    <name>A0A672Z501_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="12">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">dus4l</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5,6-dihydrouridine(20a) in tRNA + NAD(+) = uridine(20a) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53348"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:53350"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5,6-dihydrouridine(20a) in tRNA + NADP(+) = uridine(20a) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53344"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:53346"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5,6-dihydrouridine(20b) in tRNA + NAD(+) = uridine(20b) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53352"/>
            <dbReference type="Rhea" id="RHEA-COMP:13537"/>
            <dbReference type="Rhea" id="RHEA-COMP:13538"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:53354"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5,6-dihydrouridine(20b) in tRNA + NADP(+) = uridine(20b) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53356"/>
            <dbReference type="Rhea" id="RHEA-COMP:13537"/>
            <dbReference type="Rhea" id="RHEA-COMP:13538"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:53358"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 14">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Dus family. Dus4 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_030007709.1">
        <property type="nucleotide sequence ID" value="XM_030151849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z501"/>
    <dbReference type="FunCoup" id="A0A672Z501">
        <property type="interactions" value="269"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012268.1">
        <property type="protein sequence ID" value="ENSSORP00005011879.1"/>
        <property type="gene ID" value="ENSSORG00005006313.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431466"/>
    <dbReference type="CTD" id="11062"/>
    <dbReference type="InParanoid" id="A0A672Z501"/>
    <dbReference type="OrthoDB" id="9977870at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102266">
        <property type="term" value="F:tRNA-dihydrouridine20a synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000100">
        <property type="entry name" value="tRNA-dihydrouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 12 14" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="15">
        <location>
            <begin position="23"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="25"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="232"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="315" mass="35095" checksum="0B5B43FC4E283EF4" modified="2020-06-17" version="1">MKTSNGNTSVMDMFQKGKVVKVCAPMVRYSKLAFRSLVRKYNCDICFTPMIVAADFMRSVKARDSEFTTSERDRPLIVQFAAHDAQTLGDAACVVAPFSDGVDLNCGCPQRWAMSAGYGACLINKPELVKEMVRTVKNQVDNPNYTASIKIRIHKDLRQTVDLCQKAESAGVSWITVHGRTAEERHQPVHYDAIKTIKDSVAIPVIANGDIKYLRDVESTHQLTGVDGVMAARGLLANPAMFAGYEDTPLECIWDWVDISIEHGTPFTCFHHHLIYMLERVSSQPERKVFNSLSSTSAVMDYLQNTYGTVQDMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5C1</accession>
    <name>A0A672Z5C1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 19">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="15 19">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">jak1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="15 19"/>
    <dbReference type="RefSeq" id="XP_029988096.1">
        <property type="nucleotide sequence ID" value="XM_030132236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z5C1"/>
    <dbReference type="FunCoup" id="A0A672Z5C1">
        <property type="interactions" value="814"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012057.1">
        <property type="protein sequence ID" value="ENSSORP00005011672.1"/>
        <property type="gene ID" value="ENSSORG00005006192.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417981"/>
    <dbReference type="CTD" id="3716"/>
    <dbReference type="InParanoid" id="A0A672Z5C1"/>
    <dbReference type="OrthoDB" id="1915767at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005131">
        <property type="term" value="F:growth hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060397">
        <property type="term" value="P:growth hormone receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000110">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000135">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000293">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000114">
        <property type="entry name" value="Tyrosine-protein kinase JAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041155">
        <property type="entry name" value="FERM_F1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041046">
        <property type="entry name" value="FERM_F2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051286">
        <property type="entry name" value="JAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041381">
        <property type="entry name" value="JAK1-3/TYK2_PHL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016251">
        <property type="entry name" value="Tyr_kinase_non-rcpt_Jak/Tyk2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020776">
        <property type="entry name" value="Tyr_kinase_non-rcpt_Jak1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE HOPSCOTCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807:SF5">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE JAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18379">
        <property type="entry name" value="FERM_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18377">
        <property type="entry name" value="FERM_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17887">
        <property type="entry name" value="Jak1_Phl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21990">
        <property type="entry name" value="SH2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000636">
        <property type="entry name" value="TyrPK_Jak"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01823">
        <property type="entry name" value="JANUSKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01824">
        <property type="entry name" value="JANUSKINASE1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4 15 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 15 19" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="FERM" evidence="22">
        <location>
            <begin position="33"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="594"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="888"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="1016"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="894"/>
            <end position="902"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 18">
        <location>
            <position position="921"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000636-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000636-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1175" mass="135449" checksum="B6EBCB600E940C01" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z628</accession>
    <name>A0A672Z628_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z628"/>
    <dbReference type="Ensembl" id="ENSSORT00005012702.1">
        <property type="protein sequence ID" value="ENSSORP00005012305.1"/>
        <property type="gene ID" value="ENSSORG00005006496.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z628"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="72" mass="8229" checksum="713BE6AEC2C9B264" modified="2020-06-17" version="1">MPQSSAYIHNVLCQFNLQSQMTEFEASAEPLQDWLNNTEVRVQESSARLHDLPAKKQELCKLQVLGKVQKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6P1</accession>
    <name>A0A672Z6P1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115423693</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z6P1"/>
    <dbReference type="Ensembl" id="ENSSORT00005012896.1">
        <property type="protein sequence ID" value="ENSSORP00005012499.1"/>
        <property type="gene ID" value="ENSSORG00005006484.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z6P1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002504">
        <property type="term" value="P:antigen processing and presentation of peptide or polysaccharide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001003">
        <property type="entry name" value="MHC_II_a_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF86">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DR ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00993">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00920">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
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            <end position="19"/>
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            <end position="234"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012499.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="234" mass="26450" checksum="57D180813DE05411" modified="2020-06-17" version="1">MFNAITLTLLGAICTSAQGSHKLCHTTSCFKSSGQVIVTFDGDELYHADFKKEEIVWESRVPTSFHFVHAYQYAVYVRHMCKHFLKIWKGKKSAPTTAEVPELIVHSRNDVIEQIENTLICFINHFFPPVIKTKWTKNGIEVETEDSFQAFILNPDGTFHLFSTLSFIAKDGDIYGCTVEHEALTEPKTEFFEVHSNQTDNHSFVYCGICLTVGFLGIAVGTFFAVKGNKYQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z742</accession>
    <name>A0A672Z742_9TELE</name>
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        <submittedName>
            <fullName evidence="4">Leucine-rich repeat neuronal protein 4-like</fullName>
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    </protein>
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        <name evidence="4" type="primary">LOC115433612</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Apogoninae</taxon>
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    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="21"/>
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        <location>
            <begin position="22"/>
            <end position="245"/>
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        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="33"/>
            <end position="72"/>
        </location>
    </feature>
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        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="245" mass="26576" checksum="6E667C32098946E9" modified="2020-06-17" version="1">MAASWDHSFPLLIISLALIRGFTLLPTTSYAAGTIPTRPERSPDSSSDSFVVDPSDYGENETTVTPFSPDEGTTHEGPLQLCDYNLCLENQRPCAELKAATGCLCPGFTPHDQIPQVPTLRSVSWSGSEVVVWWCAPTSNITGFIVTVGGQEKQRFGLERRSGGVGNVDHITEVCVIAVNEVGESESACSMYQPRDSSLPLIAGLIGGALGFLLLLLLMVILLWRHKRQRKQETSISMHDTAATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7M9</accession>
    <name>A0A672Z7M9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Calumenin-A-like</fullName>
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    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115430858</name>
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    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with GGCX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CREC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030006984.1">
        <property type="nucleotide sequence ID" value="XM_030151124.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005012472.1"/>
        <property type="gene ID" value="ENSSORG00005006555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430858"/>
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    <dbReference type="OrthoDB" id="293868at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000109">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000110">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF76">
        <property type="entry name" value="CALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025428877" evidence="20">
        <location>
            <begin position="19"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="187"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="264"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00063649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="314" mass="36896" checksum="94AC4C1F6BBDC5B2" modified="2020-06-17" version="1">MMRPLLMFVVLCVVSASSKPTEKKDRVHHDQPLSELKHDDSQNYDYDHEAFLGHDEAQTFNELSPEESLRRLGIIVDKIDTNKDGFVSEEELKIWIKNAQKKHIYDSVENQWKDYDVNNDGHITWEEYKNVTYGSYLEDPQADIEYNYTHMMNRDERRFRVADKNGDLMTTKEEFTAFLHPEDFAHMKEIVVQETIEDIDRNGDGFIDLKEYIGDMYSPEEGKEEPEWVKSEQQQFVEFRDKNGDGKMDREETLDWILPSDYDHAEAEAKHLLSQADTNKDGKLSKEEILNKHEVFVGSQVTDFGEALLRHDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZAC4</accession>
    <name>A0A672ZAC4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">znf280d</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May function as a transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030017806.1">
        <property type="nucleotide sequence ID" value="XM_030161946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZAC4"/>
    <dbReference type="Ensembl" id="ENSSORT00005014236.1">
        <property type="protein sequence ID" value="ENSSORP00005013820.1"/>
        <property type="gene ID" value="ENSSORG00005007092.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438360"/>
    <dbReference type="CTD" id="54816"/>
    <dbReference type="InParanoid" id="A0A672ZAC4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000298">
        <property type="entry name" value="zinc finger protein 280D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059074">
        <property type="entry name" value="zf-C2H2_Z280C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057618">
        <property type="entry name" value="Znf_POGZ/Z280C-D-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF34">
        <property type="entry name" value="ZINC FINGER PROTEIN 280D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25414">
        <property type="entry name" value="zf-C2H2_Z280C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25429">
        <property type="entry name" value="zf-POGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="260"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="428"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="25"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="549"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="751"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="556"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="576"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="751"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="775"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="840" mass="93434" checksum="0ACA33396ACBD966" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZB55</accession>
    <name>A0A672ZB55_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:ch73-290k24.6</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZB55"/>
    <dbReference type="Ensembl" id="ENSSORT00005014151.1">
        <property type="protein sequence ID" value="ENSSORP00005013738.1"/>
        <property type="gene ID" value="ENSSORG00005007071.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZB55"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021684">
        <property type="entry name" value="WBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051994">
        <property type="entry name" value="WW_domain-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209:SF5">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11669">
        <property type="entry name" value="WBP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="218" mass="24456" checksum="D04855297D010BE6" modified="2020-06-17" version="1">VHHWFCPNVRLLFKGSVNAPQSSQLGSGQSRGAMEYHSGGSLPLLGRPRYCPGANANGGYLCETGHCCGETGCCTYYYELWWFWLLWTVLILFSCCCAYRHRRAKLRVQQQQRQREISLLAYHGANSYPSSMLDLSFLASLKLPSYEEVAAQPSTPPPPYSSVFTTPRYPQPPRAADPHLLTQHGPLLHRPLSDGPSSLSSDNRCHILHYINNCNLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBN3</accession>
    <name>A0A672ZBN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Regulator of telomere elongation helicase 1</fullName>
            <ecNumber evidence="16">5.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RTEL1</name>
        <name evidence="20" type="synonym">rtel1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">A probable ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZBN3"/>
    <dbReference type="FunCoup" id="A0A672ZBN3">
        <property type="interactions" value="1139"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014722.1">
        <property type="protein sequence ID" value="ENSSORP00005014300.1"/>
        <property type="gene ID" value="ENSSORG00005007319.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZBN3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </evidence>
    <sequence length="427" mass="46491" checksum="77C620B85E579B82" modified="2020-06-17" version="1">MSESKYREWILDTIDSLRSRKARPDLERICRMVRRRHGSEPDRTCAELEKLIQEQTVLKVNYKGSISYRNAAKVQRRSRKKDDVTLATASAARRSGLEEASHSDLSNGDSALGPADQDQDEEDLEADASMAMDTDSNADEEGADESRDGQDGAFPSRTYEDAPVPCPPEQAVSAPCSPSGTRPGPSPGCGPVPGSVLDCVSFQKAGERPSSLPPSSPRALAHNDWVYDSAVCSAERQHPGMQKDKAVTKPVVPSATSSPCALKSNVKKEDGVKPEDGGPSSDQLVPDYAARPDEARNGSDGRPQTLSLPSDNGTSFKKKLDMSSFTAAEDTLLNGGKNDDITVKKEKMSQNLLQWTVADVASYFSAAGFPEQAMAFRTQEIDGKSLLLMQRSDVLTGLSIRLGPALKIYERHVKVLQRTHFLEYEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFC2</accession>
    <name>A0A672ZFC2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Upstream stimulatory factor 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">usf2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZFC2"/>
    <dbReference type="FunCoup" id="A0A672ZFC2">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016490.1">
        <property type="protein sequence ID" value="ENSSORP00005015991.1"/>
        <property type="gene ID" value="ENSSORG00005007745.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZFC2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF2">
        <property type="entry name" value="UPSTREAM STIMULATORY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="217"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="268"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="327" mass="35726" checksum="91FBFDF559242D7D" modified="2020-06-17" version="1">THLQLRGNLFVQSQLRQCSERCVQLPIGEAVGTEEQTAVTITGVPQAAFTDHNVQYQFRTENSGGQVTYRVVQVTDDQIEATADGTGAVSVVSTAAFAGTPQAVAQAVIQNPFSNGGSPAGETVGGETRFAYFPAATVSDGTATAVSVQAAAEPTITQAGGQFYVMMSPPEVLQTTAPRTIAPRTHTYTADLGESEMLQHGSTNWKVDGPRAPRDERRRAQHNEVERRRRDKINNWIVTLSKIIPDCSVDSRTGASKGGILSKACDYIRELRQSNQRLQESYKEVERVEMDNELLRQQIEELKNDNALLRAQLQQHGVELNGDTTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFX9</accession>
    <name>A0A672ZFX9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger CCCH-type containing 12B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">zc3h12b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZFX9"/>
    <dbReference type="Ensembl" id="ENSSORT00005015853.1">
        <property type="protein sequence ID" value="ENSSORP00005015362.1"/>
        <property type="gene ID" value="ENSSORG00005007697.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZFX9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18729">
        <property type="entry name" value="PIN_Zc3h12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040546">
        <property type="entry name" value="Rege-1_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040757">
        <property type="entry name" value="Regnase_1/ZC3H12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF27">
        <property type="entry name" value="RIBONUCLEASE ZC3H12B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18561">
        <property type="entry name" value="Regnase_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18039">
        <property type="entry name" value="UBA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="416"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="589"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="658"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="673"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="830" mass="92964" checksum="B78605E0E5D957EF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZG62</accession>
    <name>A0A672ZG62_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZG62"/>
    <dbReference type="Ensembl" id="ENSSORT00005015938.1">
        <property type="protein sequence ID" value="ENSSORP00005015447.1"/>
        <property type="gene ID" value="ENSSORG00005007839.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZG62"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000014">
        <property type="entry name" value="Membrane cofactor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="72"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="129"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="189"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="264" mass="29518" checksum="3105A7C276199090" modified="2020-06-17" version="1">MSSWSTGRCGVPLTNPRSNSHLADKFLTRMYFASGERVHYMCDIGYGQVGGSRYRRCTNGNWSPLLLKCERRWCGSAGEISNGQFTYTGVQFGDTATAVCDEGYQLVGKPTRICVNDGWDGRVPVCEAVVCDEPPEVANAKRTEPIESSYIYRSVVAYQCLVGTIIGPKEIYCTEDGTWSSAPPECKEITCPSPNVPNASWVRAQTDQYQYKDTISIMCDPRFRIRGKSTITCGRDGKWLPEVPSCRPKPLCKSFFFFFTLCTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZG65</accession>
    <name>A0A672ZG65_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Acid sensing ion channel subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZG65"/>
    <dbReference type="Ensembl" id="ENSSORT00005016520.1">
        <property type="protein sequence ID" value="ENSSORP00005016020.1"/>
        <property type="gene ID" value="ENSSORG00005008093.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZG65"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF252">
        <property type="entry name" value="ACID-SENSING ION CHANNEL 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 11" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="9 11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="59835" checksum="D61D1F4ED3DB9761" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGH4</accession>
    <name>A0A672ZGH4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribonuclease kappa-B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115438470</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase K family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030017979.1">
        <property type="nucleotide sequence ID" value="XM_030162119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZGH4"/>
    <dbReference type="Ensembl" id="ENSSORT00005016081.1">
        <property type="protein sequence ID" value="ENSSORP00005015588.1"/>
        <property type="gene ID" value="ENSSORG00005007909.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438470"/>
    <dbReference type="InParanoid" id="A0A672ZGH4"/>
    <dbReference type="OrthoDB" id="67317at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026770">
        <property type="entry name" value="RNase_K"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31733">
        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="101" mass="10966" checksum="CD15704E47C49451" modified="2020-06-17" version="1">MPVIICGPKVAACGLVLSLWGVIMLGLLGIFFSIHSAGLIEDVPLTESDAIPGDPNVAQKIYSLYDKVSYNCFIAAGIYLLVAGFSFCQIRLNKQKEFLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZIE9</accession>
    <name>A0A672ZIE9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Iron-sulfur cluster assembly factor IBA57, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Iron-sulfur cluster assembly factor homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">iba57</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GcvT family. CAF17/IBA57 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030015614.1">
        <property type="nucleotide sequence ID" value="XM_030159754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZIE9"/>
    <dbReference type="FunCoup" id="A0A672ZIE9">
        <property type="interactions" value="716"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016775.1">
        <property type="protein sequence ID" value="ENSSORP00005016268.1"/>
        <property type="gene ID" value="ENSSORG00005008247.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436810"/>
    <dbReference type="CTD" id="200205"/>
    <dbReference type="InParanoid" id="A0A672ZIE9"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000015">
        <property type="entry name" value="IBA57, iron-sulfur cluster assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057460">
        <property type="entry name" value="CAF17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602:SF0">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25455">
        <property type="entry name" value="Beta-barrel_CAF17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CAF17 C-terminal" evidence="8">
        <location>
            <begin position="281"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="361" mass="40303" checksum="BA8DF954D0B7E463" modified="2020-06-17" version="1">MGVLCLVRGAFTSVGNLGFYARKFTGRHLCVTFHNSVRVRRYSEDTAKGGSNRGQYVCYHLPHRTLVKIHGQDTGAFLQGIITNDMRLLEENENTVIYSHMLNVQGRTLYDIMLYSMMEADAFRGVLLECDSTVKDSLLKHLKVFKIRRKVNINPCPELSVWAVLPQQKTGQEDIKPELSAPEKAVVWEADPRTQGMGWRLVTNSQVDPLDIITSCQKGNTEEYHRHRYAIGLPEGVKDLPPGVALPLESNIVYMQGISFSKGCYIGQELTARTHHTGVIRKRLMPVRLSAPVEDLEEGTALHTESGKPAGKHRAGVGELGLSLIRTAHAKEVLTVKSSDDTTVTLEASVPDWWPKDEKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJS5</accession>
    <name>A0A672ZJS5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Neutrophil cytosol factor 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Neutrophil NADPH oxidase factor 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">p40-phox</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ncf4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029977454.1">
        <property type="nucleotide sequence ID" value="XM_030121594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZJS5"/>
    <dbReference type="FunCoup" id="A0A672ZJS5">
        <property type="interactions" value="1412"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017245.1">
        <property type="protein sequence ID" value="ENSSORP00005016733.1"/>
        <property type="gene ID" value="ENSSORG00005008340.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410110"/>
    <dbReference type="CTD" id="4689"/>
    <dbReference type="InParanoid" id="A0A672ZJS5"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045730">
        <property type="term" value="P:respiratory burst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06882">
        <property type="entry name" value="PX_p40phox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11869">
        <property type="entry name" value="SH3_p40phox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000149">
        <property type="entry name" value="neutrophil cytosol factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000189">
        <property type="entry name" value="Neutrophil cytosol factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000024">
        <property type="entry name" value="Neutrophil cytosol factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000919">
        <property type="entry name" value="p40phox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035541">
        <property type="entry name" value="p40phox_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034912">
        <property type="entry name" value="PX_p40phox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF20">
        <property type="entry name" value="NEUTROPHIL CYTOSOL FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00497">
        <property type="entry name" value="P40PHOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PX" evidence="15">
        <location>
            <begin position="19"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="169"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="16">
        <location>
            <begin position="243"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="349" mass="40414" checksum="7638CD033C5FCF10" modified="2020-06-17" version="1">MSLPQQLRDESDFDQLPDNIPVTANIGDIEEKKGFIYYFRFVIEVKTKGGSKYLIYRRYREFFNLHQILEAKYSPENGEKPGPNTCVLPSLPGKVYIGNKQEIAESRIPELNNYMKKLLGLPTWFVLDEDLRMFFYQTEQDSQHQPRALRRLRPPTRKVKTVKPKMDLFSSPRAEAMFDFRGSGKAELNLKRGEVIFLLRRVNADWLEGTVNNQTGIFPESFVKIIKPLPDSDSEGEGGGHTYSCLRCFMLTPSGVDARDVCVQEDLTIQPTYKDLLSRMRNVFQVDDIALNYRDPEGDLIRILDDEDIQLMIKESRRQQGKVKRPVNQFPWELHVTMTSDLSVYNTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKH4</accession>
    <name>A0A672ZKH4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IQ motif and Sec7 domain ArfGEF 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115422239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRAG family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A672ZKH4"/>
    <dbReference type="Ensembl" id="ENSSORT00005017805.1">
        <property type="protein sequence ID" value="ENSSORP00005017289.1"/>
        <property type="gene ID" value="ENSSORG00005008658.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZKH4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13318">
        <property type="entry name" value="PH_IQSEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000001">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000004">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033742">
        <property type="entry name" value="IQSEC_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF314">
        <property type="entry name" value="IQ MOTIF AND SEC7 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16453">
        <property type="entry name" value="IQ_SEC7_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="468"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="242"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="935" mass="104401" checksum="7FFFE6E8F23338F1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZM22</accession>
    <name>A0A672ZM22_9TELE</name>
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        <submittedName>
            <fullName evidence="11">Beta 3-glucosyltransferase a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">b3glcta</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZM22"/>
    <dbReference type="FunCoup" id="A0A672ZM22">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018369.1">
        <property type="protein sequence ID" value="ENSSORP00005017844.1"/>
        <property type="gene ID" value="ENSSORG00005007884.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZM22"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000008">
        <property type="entry name" value="Beta-1,3-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="10">
        <location>
            <begin position="135"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="10">
        <location>
            <begin position="279"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="514" mass="57518" checksum="BBF44C455FCAE9C3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZM39</accession>
    <name>A0A672ZM39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 91</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cfap91</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP91 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZM39"/>
    <dbReference type="FunCoup" id="A0A672ZM39">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018385.1">
        <property type="protein sequence ID" value="ENSSORP00005017859.1"/>
        <property type="gene ID" value="ENSSORG00005008890.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZM39"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026720">
        <property type="entry name" value="CFAP91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032840">
        <property type="entry name" value="CFAP91_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455:SF10">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14738">
        <property type="entry name" value="CFAP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP91" evidence="10">
        <location>
            <begin position="173"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="649"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="425"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="669"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="693" mass="80495" checksum="7833802F6D6E1F96" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZMX5</accession>
    <name>A0A672ZMX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Conserved oligomeric Golgi complex subunit 8</fullName>
            <shortName evidence="9">COG complex subunit 8</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115436927</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Component of the conserved oligomeric Golgi complex which is composed of eight different subunits and is required for normal Golgi morphology and localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the COG8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZMX5"/>
    <dbReference type="Ensembl" id="ENSSORT00005019068.1">
        <property type="protein sequence ID" value="ENSSORP00005018521.1"/>
        <property type="gene ID" value="ENSSORG00005009141.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZMX5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016632">
        <property type="entry name" value="COG8_Metazoal_Plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015415">
        <property type="entry name" value="COG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="616" mass="69625" checksum="6274E2CC9AD02B4B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQF6</accession>
    <name>A0A672ZQF6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mgat4b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030001288.1">
        <property type="nucleotide sequence ID" value="XM_030145428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZQF6"/>
    <dbReference type="FunCoup" id="A0A672ZQF6">
        <property type="interactions" value="1952"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019974.1">
        <property type="protein sequence ID" value="ENSSORP00005019416.1"/>
        <property type="gene ID" value="ENSSORG00005008739.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427069"/>
    <dbReference type="CTD" id="11282"/>
    <dbReference type="InParanoid" id="A0A672ZQF6"/>
    <dbReference type="OrthoDB" id="2016523at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056576">
        <property type="entry name" value="MGAT4_A/B/C_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23524">
        <property type="entry name" value="MGAT4A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 conserved region" evidence="6">
        <location>
            <begin position="207"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 A/B/C C-terminal" evidence="7">
        <location>
            <begin position="497"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="133"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="72859" checksum="EEA406219D33FCDF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQZ2</accession>
    <name>A0A672ZQZ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZQZ2"/>
    <dbReference type="FunCoup" id="A0A672ZQZ2">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019832.1">
        <property type="protein sequence ID" value="ENSSORP00005019274.1"/>
        <property type="gene ID" value="ENSSORG00005009479.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQZ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    <sequence length="127" mass="14344" checksum="C49352214F55DD08" modified="2020-06-17" version="1">CGWFLCFCNVSYWKIRKSLSYLRIINYPDLSVCLSIGLSVCLSVCLSVCLSVCLYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSLLKPCIHSQVFIETDISPLPFSVMSLLGPVCVPGGQYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:centriole assembly"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="RNA-binding protein 14 isoform X1"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
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        <property type="entry name" value="RRM_euk-type"/>
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        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="6"/>
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        </location>
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        </location>
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        </location>
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            <begin position="415"/>
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        </location>
    </feature>
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        <location>
            <begin position="418"/>
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        </location>
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        <location>
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        </location>
    </feature>
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        </location>
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        <location>
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        </location>
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        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075694"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="552" mass="58707" checksum="7662A25FB72706EA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZV13</accession>
    <name>A0A672ZV13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gsx2</name>
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    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987205.1">
        <property type="nucleotide sequence ID" value="XM_030131345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZV13"/>
    <dbReference type="Ensembl" id="ENSSORT00005020643.1">
        <property type="protein sequence ID" value="ENSSORP00005020078.1"/>
        <property type="gene ID" value="ENSSORG00005009795.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417430"/>
    <dbReference type="CTD" id="170825"/>
    <dbReference type="InParanoid" id="A0A672ZV13"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000147">
        <property type="entry name" value="GS homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042191">
        <property type="entry name" value="GSH1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421">
        <property type="entry name" value="GS HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421:SF1">
        <property type="entry name" value="GS HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="134"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="136"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="238" mass="26558" checksum="1628B2AE478394DE" modified="2020-06-17" version="1">MSRSFYVDSLIIKDTVRPGPAEHHGQDFLIPISMHSPSVMTVSAPVCPSRKNGTFCVCPLCVTSHIHSSRGGIPMLKTQFPGADAQYCQRIAHQQSPALAHPGHTPVCTPTFSVTDPRRYHCLSIGASESSHIQNGKRMRTAFTSTQLLELEREFSSNMYLSRLRRIEIATYLNLSEKQVKIWFQNRRVKHKKEGKATQRSAHSSCKCTSGHTDYSRSEDEESLSPASASEEKEVSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZW62</accession>
    <name>A0A672ZW62_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized LOC115424692</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115424692</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997941.1">
        <property type="nucleotide sequence ID" value="XM_030142081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZW62"/>
    <dbReference type="Ensembl" id="ENSSORT00005022176.1">
        <property type="protein sequence ID" value="ENSSORP00005021530.1"/>
        <property type="gene ID" value="ENSSORG00005010539.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424692"/>
    <dbReference type="InParanoid" id="A0A672ZW62"/>
    <dbReference type="OrthoDB" id="2668416at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF255">
        <property type="entry name" value="ELONGIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="251"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="425" mass="47968" checksum="4758F8DAD8012AD0" modified="2020-06-17" version="1">MDISEYVLSAGRAMLDMVEREWQPLSPVELDQRLDQAVEEILEAELLAKLRAQPPPTIYVQFIQGQENVEPQVLNTEASTSSPPEEETTESTEEPVDTVDSPTVKYITDLLQSCKSRARMAGRARLSLSHTVLLSLTLLSRRVSYRSVSTRFGLEKGNIHRIFFSFCERINTLKEGQIRWPVGQEAEEHVFPLSSLLGTYRLQDQDQSVHQVLGVLGHTRIPIRLPLGKHDVDSTVPEVKRMKKEAHPDSWINLELVCDHKGRFLHCGISKGSDVDRGMTLRDKLKLHPDMLPNGSFLVARAGYPLTAQILTPYTGNLGPKEELFNKTLEACFRTLDQAVANLKARFQRLRYLDIANYDRAKAVVLTACVLHNILLDVGKEIQADVDKEEEGLTHEEEAEVDEEGVRRRAIIADLMLKNVVTENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZZT8</accession>
    <name>A0A672ZZT8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">sar1b</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus</location>
            <location evidence="15">Golgi stack membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZZT8"/>
    <dbReference type="Ensembl" id="ENSSORT00005023400.1">
        <property type="protein sequence ID" value="ENSSORP00005022735.1"/>
        <property type="gene ID" value="ENSSORG00005011021.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZZT8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1363" mass="142774" checksum="595C9505D36E7158" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A659</accession>
    <name>A0A673A659_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rho guanine nucleotide exchange factor 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Leukemia-associated RhoGEF</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">arhgef12a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="FunCoup" id="A0A673A659">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025418.1">
        <property type="protein sequence ID" value="ENSSORP00005024689.1"/>
        <property type="gene ID" value="ENSSORG00005009181.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A659"/>
    <dbReference type="OrthoDB" id="2272012at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23069">
        <property type="entry name" value="PDZ_ARHGEF11-12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000006">
        <property type="entry name" value="Rho guanine nucleotide exchange factor (GEF) 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000033">
        <property type="entry name" value="Rho guanine nucleotide exchange factor (GEF) 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000072">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000008">
        <property type="entry name" value="rho guanine nucleotide exchange factor 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041020">
        <property type="entry name" value="PH_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015212">
        <property type="entry name" value="RGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45872:SF3">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45872">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 2, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17838">
        <property type="entry name" value="PH_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09128">
        <property type="entry name" value="RGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="76"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="15">
        <location>
            <begin position="798"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="1030"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="284"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="599"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="736"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1170"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="344"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="674"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="740"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1182"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1218"/>
            <end position="1227"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1440" mass="160233" checksum="E53092102BA8E1A9" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A7N9</accession>
    <name>A0A673A7N9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115437010</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
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    </dbReference>
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        <property type="component" value="Chromosome 17"/>
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    <dbReference type="GO" id="GO:0005874">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23649">
        <property type="entry name" value="Khc_CBD_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059182">
        <property type="entry name" value="Khc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF68">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="8"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="868"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="379"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="890"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="90"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="926" mass="105900" checksum="4D88F60963004E4E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AB24</accession>
    <name>A0A673AB24_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">G1 to S phase transition 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AB24"/>
    <dbReference type="Ensembl" id="ENSSORT00005027164.1">
        <property type="protein sequence ID" value="ENSSORP00005026389.1"/>
        <property type="gene ID" value="ENSSORG00005012652.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AB24"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000024">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000270">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="145"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="81"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="549" mass="60618" checksum="64A459883BE59BF8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABG3</accession>
    <name>A0A673ABG3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nsun5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029994519.1">
        <property type="nucleotide sequence ID" value="XM_030138659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ABG3"/>
    <dbReference type="SMR" id="A0A673ABG3"/>
    <dbReference type="Ensembl" id="ENSSORT00005027703.1">
        <property type="protein sequence ID" value="ENSSORP00005026920.1"/>
        <property type="gene ID" value="ENSSORG00005012888.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422362"/>
    <dbReference type="CTD" id="55695"/>
    <dbReference type="InParanoid" id="A0A673ABG3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1170:FF:000004">
        <property type="entry name" value="probable 28S rRNA (Cytosine-C(5))-methyltransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049561">
        <property type="entry name" value="NSUN5_7_fdxn-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21148">
        <property type="entry name" value="NSUN5_fdxn-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="124"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="231"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="496" mass="55129" checksum="7F61EC40DDAADFA6" modified="2020-06-17" version="1">MALYVKAAEILQKAEKKQGALKTLVYDSKFANIKQLFALVCETQKFSSILQEIIASTKLLKLTGLKPHLAQVLVYDLIIGQGLKCGGSWKAMLMKHRSRLQAELARMKVKQKVSRNQDLLPASLQQSAADLLPRYVRVNTLKTTVEDAVDYLKREGFLYLGQASRLDDLNLKKKAFVQDLHLPEVLVFSPKMDFHDHFLYKAGHIILQDKASCLPAHILNPPPGSVVIDACAAPGNKTSHLAAIMNNKGQLFAFDLDAKRLATMSTLLLRAGVTCQQLANQDFLTVDPESPQYKDVEYILLDPSCSGSGMVCLRDNSSSADVEKDQARLASLASFQLRCLNHGLRFPRLKSLVYSTCSIHSQENEDVISACLQQNPSFRLVPLLPQWPERGLEPFTQCLRASTSKTRTHGFFVALLEKRSESEPVDQEPAVQISSEVTSHSPPKGEKRPAPSPEESEASDTEEKPEPAAEQMNDAQAKKRRKRKKKKKKKAAASAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABP7</accession>
    <name>A0A673ABP7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Guanine nucleotide-binding protein G(s) subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Adenylate cyclase-stimulating G alpha protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gnas</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the G-alpha family. G(s) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ABP7"/>
    <dbReference type="Ensembl" id="ENSSORT00005027393.1">
        <property type="protein sequence ID" value="ENSSORP00005026614.1"/>
        <property type="gene ID" value="ENSSORG00005012460.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABP7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031698">
        <property type="term" value="F:beta-2 adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051430">
        <property type="term" value="F:corticotropin-releasing hormone receptor 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031748">
        <property type="term" value="F:D1 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005159">
        <property type="term" value="F:insulin-like growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031852">
        <property type="term" value="F:mu-type opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007191">
        <property type="term" value="P:adenylate cyclase-activating dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000003">
        <property type="entry name" value="Guanine nucleotide-binding protein G(S) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:006178">
        <property type="entry name" value="Guanine nucleotide-binding protein G(s) subunit alpha isoforms short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000367">
        <property type="entry name" value="Gprotein_alpha_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF357">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(S) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00443">
        <property type="entry name" value="GPROTEINAS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 13 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="73"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="206"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="231"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="300"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="402" mass="46902" checksum="F76CB237CCCF8E5A" modified="2020-06-17" version="1">STVLASTSSFPKQKKGVKFVLCQMKVLRIIFKKKCFPTLCTAIDVCVCLVLHVNKSLTVYLIFCFFFLSGAGESGKSTIVKQMRILHVNGFNAEEKKQKIQDIKNNIKEAIETIVTAMSTLTPPVQLACPENQYRIEYVLNLVNQKDFEFPSEFYDHAKTLWQDEGVRACYERSNEYQLIDCAQYFLDKIDIVKQSDYTPTDQDLLRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKNIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEEYFPEFARYTTPDDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACC8</accession>
    <name>A0A673ACC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cyclin-dependent kinase 20</fullName>
            <ecNumber evidence="5">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Cell cycle-related kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Cell division protein kinase 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">cdk20</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="5"/>
    <dbReference type="RefSeq" id="XP_029995147.1">
        <property type="nucleotide sequence ID" value="XM_030139287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ACC8"/>
    <dbReference type="FunCoup" id="A0A673ACC8">
        <property type="interactions" value="513"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028026.1">
        <property type="protein sequence ID" value="ENSSORP00005027240.1"/>
        <property type="gene ID" value="ENSSORG00005013008.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422764"/>
    <dbReference type="CTD" id="23552"/>
    <dbReference type="InParanoid" id="A0A673ACC8"/>
    <dbReference type="OrthoDB" id="63265at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07832">
        <property type="entry name" value="STKc_CCRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000406">
        <property type="entry name" value="cyclin-dependent kinase 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000211">
        <property type="entry name" value="Putative cyclin-dependent kinase 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048002">
        <property type="entry name" value="CDK20-like_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF171">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 23 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 23 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 24" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="6"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00035723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="346" mass="38871" checksum="0E54A7E24A065D31" modified="2020-06-17" version="1">MNMEQYSILGRIGEGAHGIVFKAKHIETGETVALKKVALRRLEDGIPNQALREIKALQEIEDNQHVVKLKDVFPHGTGFVLVFDFMLSDLSEVIRNSQRPLTPAQVKGYMMMLLKGVAFLHHNNVMHRDLKPANLLISSSGHLKIADFGLARLLSEQSDRLYSHQVATRWYRAPELLYGARKYDEGVDLWAVGCIFGELLNSSPLFPGENDIEQLCCVLRVLGTPTQDSWPDIVELPDYNKITFKENPAIPLEEIVPDTSPQAIDLLYKFLVYPSKQRCSASQALLHPYFFTSPLPAHHSELPIPQRGGRPPRQRLQAPPTDFSVDLPLQDSVVDPALLQEHASCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AE11</accession>
    <name>A0A673AE11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="13">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115438132</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A673AE11"/>
    <dbReference type="FunCoup" id="A0A673AE11">
        <property type="interactions" value="978"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028258.1">
        <property type="protein sequence ID" value="ENSSORP00005027469.1"/>
        <property type="gene ID" value="ENSSORG00005013122.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AE11"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000307">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.670:FF:000001">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000003">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21292">
        <property type="entry name" value="EME1-MUS81_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21136">
        <property type="entry name" value="WHD_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="15">
        <location>
            <begin position="265"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="181"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="556" mass="61827" checksum="EF75B2FA9883F5FF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AE42</accession>
    <name>A0A673AE42_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="17">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">scamp5a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the SCAMP family. SCAMP5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AE42"/>
    <dbReference type="FunCoup" id="A0A673AE42">
        <property type="interactions" value="240"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027782.1">
        <property type="protein sequence ID" value="ENSSORP00005026998.1"/>
        <property type="gene ID" value="ENSSORG00005012924.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AE42"/>
    <dbReference type="OrthoDB" id="242866at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF5">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="230" mass="25594" checksum="E4BA48FDD6AB4FE1" modified="2020-06-17" version="1">MAENNFPPLPRFIPLKPCFYQDFNEIPDQRRTMCKRLYYLWILNSATLAVNLIGCLAWMCGGGGATNFGMAILWLILFTPCSYVCWFRPIYKAFKTDSSFNFMAFFFVFMAQVVISIIQTVGIPGWGVCGWLATITFFSTNIGSAVVMLIPTIMFTAVAVLSFIALTKVHNFYRGSGGSLGKAQEEWATGAWKNPHVQQAAQQAAMGAAQGAMQQNQYSAAPTYNYDDPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEB3</accession>
    <name>A0A673AEB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zona pellucida sperm-binding protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Zona pellucida glycoprotein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Zona pellucida protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC115421032</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Zona pellucida</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029992547.1">
        <property type="nucleotide sequence ID" value="XM_030136687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AEB3"/>
    <dbReference type="Ensembl" id="ENSSORT00005028740.1">
        <property type="protein sequence ID" value="ENSSORP00005027940.1"/>
        <property type="gene ID" value="ENSSORG00005012903.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421032"/>
    <dbReference type="InParanoid" id="A0A673AEB3"/>
    <dbReference type="OrthoDB" id="8919081at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060468">
        <property type="term" value="P:prevention of polyspermy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017977">
        <property type="entry name" value="ZP_dom_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF31">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00682">
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0278">Fertilization</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025330253" description="Zona pellucida sperm-binding protein 4" evidence="19">
        <location>
            <begin position="23"/>
            <end position="432"/>
        </location>
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        <location>
            <begin position="61"/>
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        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="20">
        <location>
            <begin position="104"/>
            <end position="399"/>
        </location>
    </feature>
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            <begin position="31"/>
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    </feature>
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        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
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        <location>
            <begin position="63"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00037545"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040238"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51034"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027940.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="432" mass="47979" checksum="30F7D9C6C7C46D77" modified="2020-06-17" version="1">MASKLISGGLFAIAFLGCLVNAQLDTNQTAQNIHDPQPQTPATQSQNTQSKSHPDTQNRRSSCVVEQQNKIQCGAPGTSATVCNALNCCFDGTMCYYGKYVTLHCTKDAQMIVIVSKDATIPNLDLESVGLLSKTGNCSPVATTSAFAIYQFPVTECGTVMSEEDGVLTYENRMFSQYEVELGPRGAITRDSFFDLSIQCRYTGSTAEALLLEMNILPSPYPAVGFGILNVELRLANGQCHLKGCAEEDVAYSSYYRDSEYPITKELQDNVYVEVRMLGRTDPNLVLTLGHCWATSKSNPLSRPQWDLLINGCPYREDRYQTKLLNVGSKLMFPSHYRRFAFQMFTFVSTGAADPSKGNQVTQTADTVNPTYEKVYIHCNVAVCQPSTQYNCEPRCFRKRRDIGESKTFTSRQEDRTVSSQEIQFVALSQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AG03</accession>
    <name>A0A673AG03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">hdac5</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="AlphaFoldDB" id="A0A673AG03"/>
    <dbReference type="FunCoup" id="A0A673AG03">
        <property type="interactions" value="861"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029004.1">
        <property type="protein sequence ID" value="ENSSORP00005028201.1"/>
        <property type="gene ID" value="ENSSORG00005013364.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AG03"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10164">
        <property type="entry name" value="ClassIIa_HDAC5_Gln-rich-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000002">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046949">
        <property type="entry name" value="HDAC4/5/7/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF12">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037911">
        <property type="entry name" value="HDAC_II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="19">
        <location>
            <begin position="81"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="18">
        <location>
            <begin position="588"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="219"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="309"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="124"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="717"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="580"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="665"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Contributes to catalysis" evidence="15">
        <location>
            <position position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1023" mass="112493" checksum="9E25953D1DCCC7D8" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AHK4</accession>
    <name>A0A673AHK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AHK4"/>
    <dbReference type="Ensembl" id="ENSSORT00005028613.1">
        <property type="protein sequence ID" value="ENSSORP00005027817.1"/>
        <property type="gene ID" value="ENSSORG00005013308.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AHK4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="114" mass="13371" checksum="11DBB1A62EB8269D" modified="2020-06-17" version="1">MTPALSRRIQLTVRQDTGRSSTQIKALTGADCSAITIRRHLREKGFKNKKRIQRPLLLQCHKTARLDFAREHQTWDIERWKKPISVYLLFSINYFFKVLFVSLPLLAFVYCSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AK45</accession>
    <name>A0A673AK45_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fosl1a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. FOS has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AK45"/>
    <dbReference type="Ensembl" id="ENSSORT00005030917.1">
        <property type="protein sequence ID" value="ENSSORP00005030075.1"/>
        <property type="gene ID" value="ENSSORG00005014356.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AK45"/>
    <dbReference type="OrthoDB" id="5866312at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14721">
        <property type="entry name" value="bZIP_Fos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000006">
        <property type="entry name" value="fos-related antigen 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF25">
        <property type="entry name" value="FOS-RELATED ANTIGEN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="129"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="109"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="292"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00058242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="343" mass="37357" checksum="4CA9EC62573C210A" modified="2020-06-17" version="1">MYRNFGNQDRGSPSYTGSGSGTVSLGSSSTSTTQQEQKFTMAGSSQFVPSLNAITSNQDLQWMVQPSLMHPPGPSQSPVPPYPSVSRVRPLGPPLSQSHLLRPGVIRAAANTTTSTRRRNDEHLSHEELERRRVRRERNKLAAAKCRNRRRELTDSLQSETDELEDEKSRLQKEIADLQKEKEKLELVLEAHRPICKIEESDSDSDPNPSLSSLEGIKMESVGRSRPGPSTKPPVKMEKPKPKITIPTKGASTASTVASESESLHTPVLTSTPSLTPITYGMVFTYPSAALDTSASTSSHGTSHHGSVHQSQAPRPCGIAHRRSSSSGDQSDHSLHSPTILTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMX6</accession>
    <name>A0A673AMX6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Nephronectin a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">npnta</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nephronectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AMX6"/>
    <dbReference type="Ensembl" id="ENSSORT00005031472.1">
        <property type="protein sequence ID" value="ENSSORP00005030614.1"/>
        <property type="gene ID" value="ENSSORG00005014602.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AMX6"/>
    <dbReference type="OrthoDB" id="10060424at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000187">
        <property type="entry name" value="nephronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000476">
        <property type="entry name" value="nephronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000184">
        <property type="entry name" value="nephronectin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000268">
        <property type="entry name" value="nephronectin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003882">
        <property type="entry name" value="Pistil_extensin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF19">
        <property type="entry name" value="NEPHRONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01218">
        <property type="entry name" value="PSTLEXTENSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025538592" description="Nephronectin a" evidence="15">
        <location>
            <begin position="20"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="138"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="218"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="263"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="17">
        <location>
            <begin position="473"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="350"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="390"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="425"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="69233" checksum="48BFDFEEF8BD0E8F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQ09</accession>
    <name>A0A673AQ09_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">AP-3 complex subunit mu-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ap3m1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AQ09"/>
    <dbReference type="FunCoup" id="A0A673AQ09">
        <property type="interactions" value="715"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031352.1">
        <property type="protein sequence ID" value="ENSSORP00005030497.1"/>
        <property type="gene ID" value="ENSSORG00005014543.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQ09"/>
    <dbReference type="OrthoDB" id="870at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14837">
        <property type="entry name" value="AP3_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000012">
        <property type="entry name" value="AP-3 complex subunit mu-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="10">
        <location>
            <begin position="176"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="418" mass="46742" checksum="AB600F693409F7B1" modified="2020-06-17" version="1">MIHSLFLINHTGDIFLEKHWKSVISRSVCDYFFEAKEKAGDPENVPPVLQTPHHYLITIYRGKLFLLSVIQTEVPPLFVIEFLHRVADTFQDYFGECSEGVIKDNVVTVYELLEEMLDNGFPLATESNVLKEMIKPPNILRSVVNTLTGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVIEEIDAILDKSGTTVFAEIQGVIEACVRLTGMPDLTLSFMNPRLLDDVSFHPCVRFKRWEAERVLSFIPPDGNFTLMTYHVSSQNLVAIPVYVKQSISFFESGSCGRLDITLGPKQTMGKMVEGLTVTIHMPKTVLSANLTATQGNYTYDLATKVLVWDIGKLNPQKLPNLKGSLSVQAGAPKPEENPSLNIGLKIQQLAISGLKVSRLDMYGEKYKPFKGVKYVTKAGKFQVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARW3</accession>
    <name>A0A673ARW3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">evx2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the even-skipped homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029981463.1">
        <property type="nucleotide sequence ID" value="XM_030125603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ARW3"/>
    <dbReference type="FunCoup" id="A0A673ARW3">
        <property type="interactions" value="528"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033163.1">
        <property type="protein sequence ID" value="ENSSORP00005032276.1"/>
        <property type="gene ID" value="ENSSORG00005015316.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412926"/>
    <dbReference type="CTD" id="344191"/>
    <dbReference type="InParanoid" id="A0A673ARW3"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000454">
        <property type="entry name" value="Even-skipped homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052002">
        <property type="entry name" value="Even-skipped_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294:SF1">
        <property type="entry name" value="HOMEOBOX EVEN-SKIPPED HOMOLOG PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294">
        <property type="entry name" value="SEGMENTATION PROTEIN EVEN-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="164"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="166"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="415" mass="44522" checksum="0246C699CE3B2530" modified="2020-06-17" version="1">MMERIRKEMILMERGLHSPVAGKRLSDAPGNSVLEALENSQHGGRLSPRITSASLHGNLGDIPTKGKFEIDSLFGTHHNSENTSSAEISSSESRKKMSLYSEVSQESDINSDVEVGCPAHRSPSQHKENNKGFSDSNSASSNTSSSSLPNMNGNSAGSNNSNSDQVRRYRTAFTREQIGRLEKEFYRENYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAMSWPHPADPSFYTYMMTHAAATGSLPYPFHSHMPLHYYPHVGVTAAAAAAAATGAASSPFATSIRPLDTFRALSHPYSRPELLCSFRHPGLYQSPAGLNSSAAASAAAAAAAAAAAVSAPSATGPCSCLSCHSSQAASALGSRSTSADFTCTASGQRSESGFLPYSAAVLSKTSVPSPDQREETSLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AV11</accession>
    <name>A0A673AV11_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GDNF family receptor alpha 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AV11"/>
    <dbReference type="Ensembl" id="ENSSORT00005033074.1">
        <property type="protein sequence ID" value="ENSSORP00005032187.1"/>
        <property type="gene ID" value="ENSSORG00005015292.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AV11"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF4">
        <property type="entry name" value="GDNF FAMILY RECEPTOR ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="6">
        <location>
            <begin position="33"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="190" mass="21724" checksum="ED86ED46E45841D8" modified="2020-06-17" version="1">LISHLFFHYFSHPDVCVCDSELAPPGWREPLDCVRASELCNQNSQCSSRYRIMRQCLSGGDKERSTMLASKECQGALEVLQDSPLYDCRCKRGMKKELQCLQNYWSIHMGLTEGEEFYETSPYEPIHFSEEFQLASIISGTPCNPCLDAAKACNLNDTCKRLRSSYTTICSKMSLCFGKSRSTFKFLRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWS6</accession>
    <name>A0A673AWS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Dynein light chain Tctex-type 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Dynein light chain Tctex-type 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC115415883</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Probably binds BUB3 as part of transport cargo. Required for the efficient progression through mitosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. DYNLT1 and DYNLT3 compete for association with dynein IC (DYNC1I1 or DYNC1I2). Self-associates. Interacts with DYNC1I1 and DYNC1I2. Interacts with BUB3. Interacts with SATB1 in nucleus to form complex with matrix attachment regions (MARs) of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dynein light chain Tctex-type family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029985404.1">
        <property type="nucleotide sequence ID" value="XM_030129544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AWS6"/>
    <dbReference type="Ensembl" id="ENSSORT00005035077.1">
        <property type="protein sequence ID" value="ENSSORP00005034161.1"/>
        <property type="gene ID" value="ENSSORG00005016144.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415883"/>
    <dbReference type="InParanoid" id="A0A673AWS6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.40:FF:000001">
        <property type="entry name" value="Dynein light chain Tctex-type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF20">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="18" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0243">Dynein</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-0944">Nitration</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00039901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00046439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00072460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="117" mass="12910" checksum="52F38ADB9259F391" modified="2020-06-17" version="1">MNGMEEFQNGSEGSFNSEEAESLVKECIDAVVGGDDYSQTHVNKWTASVVERTLSTLVKQGKPYKYMVTCAVMQKTGAGLHTANSCYWDTTMDGSCTVRWENRTMYCVVSVFAVAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AX74</accession>
    <name>A0A673AX74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AX74"/>
    <dbReference type="Ensembl" id="ENSSORT00005033858.1">
        <property type="protein sequence ID" value="ENSSORP00005032957.1"/>
        <property type="gene ID" value="ENSSORG00005015618.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AX74"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043277">
        <property type="term" value="P:apoptotic cell clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060097">
        <property type="term" value="P:cytoskeletal rearrangement involved in phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608:SF2">
        <property type="entry name" value="T CELL IMMUNOGLOBULIN AND MUCIN DOMAIN CONTAINING 4 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="25789" checksum="07771C573D395460" modified="2020-06-17" version="1">MFCLPLSGCWFSSVSPFKVTEGGRFGLSRVCWGRGCGTLWCSNILVQTDENGVISKVADRYRLSGDILDGQMDLDILNVRRTDSGPYCCRVDIDGIFNDKKVIMNLRVVKAPVTSPPTTTTMTTAPTDPVTELSASTGEPDIVLSPQSEIYLISVFQVEDSLPSLSLQINVPILSLSLSVLMILAAVLLFLAFKRGIYRRALKTGCFSSDEPPHIIYEIRMRRPVQENIYTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AXI7</accession>
    <name>A0A673AXI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115428051</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AXI7"/>
    <dbReference type="FunCoup" id="A0A673AXI7">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035391.1">
        <property type="protein sequence ID" value="ENSSORP00005034471.1"/>
        <property type="gene ID" value="ENSSORG00005016267.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AXI7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF60">
        <property type="entry name" value="GAP JUNCTION ALPHA-9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="14">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="15">
        <location>
            <begin position="167"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="313"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="382"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="448" mass="49931" checksum="862ABD231606F76A" modified="2020-06-17" version="1">MGDWNFLGGILEEVHIHSTMVGKIWLTILFIFRMLVLGVAAEDVWNDEQSDFICNTDQPGCRNVCYDQAFPISLIRYWVLQVIFVSSPSLVYMGHAIYQLRALEKERHCKKVALRRELEAVDMELVEVRRRIEKEMRQLEQGKLNKAPLRGSLLCTYVAHIVTRSVVEVSFMMGQYILYGHHLNPLYKCEREPCPNVVDCFVSRPTEKTVFMMFMQAIACISLFLSLLEIMHLGFKRLKRGILDYYPHLKDDLDDYYSNKSKKNSVVHQVCMGTNVGRKTTIPTAPSGYTLLLEKQGNGPNYPLLNASSAFVPIQGDSGAKPDHHKDSKAGVPSPTEQNSNSNNTSSETRSPPYPTLPVADTSSCTTLSGLARKSRRVSPPWNCSTVVEGNGSDSGDSYHGGNGMKLRSSCVGPRARMSNSPTASSPNRRVSAASSGSSRRAPTDLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZS8</accession>
    <name>A0A673AZS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Methylcytosine dioxygenase TET</fullName>
            <ecNumber evidence="13">1.14.11.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tet1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming during embryonic development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>a 5-formyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-carboxyl-2'-deoxycytidine in DNA + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53832"/>
            <dbReference type="Rhea" id="RHEA-COMP:13656"/>
            <dbReference type="Rhea" id="RHEA-COMP:13657"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:137731"/>
            <dbReference type="ChEBI" id="CHEBI:137732"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>a 5-hydroxymethyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-formyl-2'-deoxycytidine in DNA + succinate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:53828"/>
            <dbReference type="Rhea" id="RHEA-COMP:13315"/>
            <dbReference type="Rhea" id="RHEA-COMP:13656"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:136731"/>
            <dbReference type="ChEBI" id="CHEBI:137731"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5-methyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-hydroxymethyl-2'-deoxycytidine in DNA + succinate + CO2</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
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    <sequence length="2180" mass="238851" checksum="FA938C35940FE550" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1H7</accession>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="103" mass="11788" checksum="2090544D8D369001" modified="2020-06-17" version="1">MAHYRASESKREQFRRYLEKSGVVDTITNVLVALYEDPDKPNNALEFIKLHLGAAGPEPTDIEALRMELADLQEKCNLLLEENKELRNRLMQYEPSPDEGAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B1N7</accession>
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        <recommendedName>
            <fullName evidence="7">G2/mitotic-specific cyclin-B2</fullName>
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            <taxon>Apogonoidei</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="1">Essential for the control of the cell cycle at the G2/M (mitosis) transition.</text>
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        <text evidence="6">Interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.</text>
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        <text evidence="2">Belongs to the cyclin family. Cyclin AB subfamily.</text>
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        <property type="entry name" value="G2/mitotic-specific cyclin"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8" id="KW-0195">Cyclin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="9">
        <location>
            <begin position="39"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="267" mass="30867" checksum="6B19B8A8D4BD8D9C" modified="2020-06-17" version="1">MFVSSRSGSWMEVNSHRNSSRFLQMHPRIEPRMRSILLDWMMEVSEAFTLQRHTFYLAQDYFDRFMQRQYNVQKESLQVIGATCLFLASKMEESRAVKLKQVVDVGDGAYTEQDVRRVELLILKTLGWRLSSDTALFWLELYLQLVLMEVLDLCMLHVNSMDFEYNVLAAAILSRFFHQEVVEQVSGLSGGDIEACISWVIPFVSAACLFGEETVPKFVKIHPEEGHNIQTHTDYLTMLHSGHLSSIPYNAIHTITVLDQPYLQLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3P4</accession>
    <name>A0A673B3P4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 710</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">znf710b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B3P4"/>
    <dbReference type="Ensembl" id="ENSSORT00005037378.1">
        <property type="protein sequence ID" value="ENSSORP00005036415.1"/>
        <property type="gene ID" value="ENSSORG00005017168.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3P4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000161">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000182">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000186">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000191">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000203">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000451">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000502">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000649">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF57">
        <property type="entry name" value="ZINC FINGER PROTEIN 154-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="570"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="598"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="77"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="169"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="246"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="690" mass="77059" checksum="F1FA73FFB897151E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B497</accession>
    <name>A0A673B497_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 13">Histone deacetylase</fullName>
            <ecNumber evidence="2 13">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">hdac2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-(2E)-butenoyl-L-lysyl-[protein] + H2O = (2E)-2-butenoate + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:69172"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:35899"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:69173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:58197"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-acetyl-L-lysyl-[protein] + H2O = L-lysyl-[protein] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:58109"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 13"/>
    <dbReference type="AlphaFoldDB" id="A0A673B497"/>
    <dbReference type="Ensembl" id="ENSSORT00005036283.1">
        <property type="protein sequence ID" value="ENSSORP00005035333.1"/>
        <property type="gene ID" value="ENSSORG00005016645.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B497"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:NuRD complex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HDAC_I/II"/>
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        <property type="entry name" value="HDACs"/>
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        <property type="entry name" value="His_deacetylse_dom"/>
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        <property type="entry name" value="His_deacetylse_dom_sf"/>
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        <property type="entry name" value="Ureohydrolase_dom_sf"/>
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        <property type="entry name" value="HISTONE DEACETYLASE 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HDASUPER"/>
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    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
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        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="8 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="18">
        <location>
            <begin position="30"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="384"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="396"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="439"/>
            <end position="473"/>
        </location>
    </feature>
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        <location>
            <position position="143"/>
        </location>
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        <location>
            <position position="101"/>
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        <ligand>
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        <ligand>
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        <location>
            <position position="178"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:60240"/>
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            <dbReference type="ARBA" id="ARBA00022853"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF00850"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035333.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="473" mass="53881" checksum="1CDA1DF4AC9C9E18" modified="2020-06-17" version="1">MAYTTAGGTKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKATAEEMTKYHSDDYIKFLRSIRPDNMSEFSKQMQRFNVGEDCPVFDGLFEFCQLSAGGSAAGSVKLNRQQTDIAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNFPLRDGIDDESYEQIFKPVMAKVMEMYQPSAVVLQCGADSLSGDRLGCFNLTIRVHEVLQPALLMLGGGGYTIRNVARCWTYETAVALDTDIPDELPYNDYFEYFGPDFKLHISPSNMTNQNTQEYMDKIKQRLFENLRMLPHAPGVQMQAIPEDAVPDDTVDEDTEDPDKRLSIRATDKRIACDEEFSDSEDEGEGGRRNVANHKKGAKRPRVDEDKKEGEEKKAEVKEEEKTKDSSAEKTESKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4T0</accession>
    <name>A0A673B4T0_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">5'-nucleotidase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="primary">LOC115415550</name>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="LD24878p"/>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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        <property type="entry name" value="HAD_sf"/>
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        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF38">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 1"/>
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    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00069357"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="325" mass="36504" checksum="1724041CD59E98A9" modified="2020-06-17" version="1">IGQLSTKSYTVFGRGLLNLATEVQSWHQCVQLSNCLCACVCFTDDAGTYFPSVKRDPGRYLQPCSDSVKTWLHSMKNAGKVLLLITSSHSDYCRLICEHILGKDFEELFDVIITNALKPGFFSLVPQQRPFRTLVNDVEESEGLPSLDKPGWYSQGNWPHLHELLKTMTGKPEPKVVYFGDSMRSDMFPASSFGKWETVMIVEEMEGEGVPKSDAAKSNEAQVEPLEKKGKFEQGMKSPSAISNQWGSYFVDVHQSGGGDEESQKLTWCCHCIHKYSTMAIPSVEHIADLPLDYKFPRFSPDKPCTTGYYPRPPDSLLKRCESMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B717</accession>
    <name>A0A673B717_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DENN domain containing 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dennd10</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DENND10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B717"/>
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        <property type="protein sequence ID" value="ENSSORP00005037630.1"/>
        <property type="gene ID" value="ENSSORG00005017661.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FAM45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28544:SF1">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28544">
        <property type="entry name" value="PROTEIN FAM45A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08616">
        <property type="entry name" value="SPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="332" mass="37658" checksum="4A8C3B08114285B6" modified="2020-06-17" version="1">MGLIKLISPHYSIKKDVNGDTLWVWCYPSVDSQLRQFCRTWYYITTVEVQEPTALSKVQCDSFSIVVTAKDFNPEKYAALSRILCRMYVKHGSPVKMMEAYITVLTKGICQSDENGSFLIKDYDVRKAYLAGSVKVKSDVVSQFGMETIILYTALMLKKRIVVHHPRIEALLEFTRVLPALTWHRKDWSILHPYVHLTETELEDLSRCPGYVAGFVDPEVSNRSDLFDVYVNLPDSVITVSQSAKEAMAMGKLHKDIGHLIVQSAEDTDRSDSQVIKDISVKTKEILSNLVSLANECDDSKLTLDALKQHHFPPATENFLFHLAAAEQLLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAX2</accession>
    <name>A0A673BAX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Regulator of microtubule dynamics protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein FAM82A1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rmdn2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RMDN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030012540.1">
        <property type="nucleotide sequence ID" value="XM_030156680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BAX2"/>
    <dbReference type="Ensembl" id="ENSSORT00005040540.1">
        <property type="protein sequence ID" value="ENSSORP00005039531.1"/>
        <property type="gene ID" value="ENSSORG00005018436.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434638"/>
    <dbReference type="CTD" id="151393"/>
    <dbReference type="InParanoid" id="A0A673BAX2"/>
    <dbReference type="OrthoDB" id="512473at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097431">
        <property type="term" value="C:mitotic spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049039">
        <property type="entry name" value="RMD1-3_a_helical_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056:SF15">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21033">
        <property type="entry name" value="RMD1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="411" mass="45974" checksum="3D19A55FA606967F" modified="2020-06-17" version="1">MAQADSKVLVLGALAGVAGVSLAVAWYKGYKSKRRASMPAYYLNCTNERAGNLMFVDSPSLAPGQAEVLDRLEALIQCVSELKDEMKALKNALPTLQDQVREELRGRERAEARRVSPLHRTTPTRRKRPAGIVSGSRSGGRSSEEAESEGGYITALTDSDEEELSDAEQRDEEQPADKLSILLERIDRLHQGTDSDKRESLSILLEQQEEFGQNSTFLWRLIRAYCDLHDASSTTEEKKTHAETGKRVGEEAVRLNPTCAESHQWYAIMCGIMAEYDTVQNKIKNGYIFKDHLDKAIELNPQDPLSYYLLGRWCYAVAQLSWLERKVAATLFGEPPSATVEDALKNFLKVEEIHPGYSKLNYVFLAKCYKDLGQRDKARKMCEAACSMNAIAKEADEAQKELDVLCPLLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB15</accession>
    <name>A0A673BB15_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Amyloid beta (A4) precursor-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115431029</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BB15"/>
    <dbReference type="Ensembl" id="ENSSORT00005038799.1">
        <property type="protein sequence ID" value="ENSSORP00005037812.1"/>
        <property type="gene ID" value="ENSSORG00005017716.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038802.1">
        <property type="protein sequence ID" value="ENSSORP00005037815.1"/>
        <property type="gene ID" value="ENSSORG00005017716.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038804.1">
        <property type="protein sequence ID" value="ENSSORP00005037817.1"/>
        <property type="gene ID" value="ENSSORG00005017716.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038806.1">
        <property type="protein sequence ID" value="ENSSORP00005037819.1"/>
        <property type="gene ID" value="ENSSORG00005017716.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21709">
        <property type="entry name" value="JMTM_APLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22607">
        <property type="entry name" value="Kunitz_ABPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000003">
        <property type="entry name" value="amyloid-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF14">
        <property type="entry name" value="AMYLOID BETA PRECURSOR LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00203">
        <property type="entry name" value="AMYLOIDA4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627734" evidence="14">
        <location>
            <begin position="21"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="684"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="16">
        <location>
            <begin position="35"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="15">
        <location>
            <begin position="304"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="17">
        <location>
            <begin position="368"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="10">
        <location>
            <begin position="35"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="10">
        <location>
            <begin position="138"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="197"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="583"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="80"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="105"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="140"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="165"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="753" mass="85533" checksum="898259CE021672A5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB93</accession>
    <name>A0A673BB93_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BB93"/>
    <dbReference type="Ensembl" id="ENSSORT00005040668.1">
        <property type="protein sequence ID" value="ENSSORP00005039656.1"/>
        <property type="gene ID" value="ENSSORG00005018499.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BB93"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:immunological synapse formation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF1">
        <property type="entry name" value="PERFORIN 1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025490371" description="MACPF domain-containing protein" evidence="8">
        <location>
            <begin position="30"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="9">
        <location>
            <begin position="25"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="425" mass="48209" checksum="142EC5585641DFCB" modified="2020-06-17" version="1">MGKTVLDLIIHLIQHLFFCANCVWDYAQCRRAKFVPGYNLVGEGFDVVTLERKGAYLINVGAYMTPRGSCRLYRNPHQGNYLQKLPISVADWRPVTHCEVHLEDTVHSSARYSVTKHTITCSVGSIVPFTGRSEDAVGLHGQGQHRAHQNVLIFRFRAISAPRLSWEFRKDLERLPSYYNSSSEDEYRNIIDTYGTHYIRKVDLGGQIQRFTATRTCLSKLNGFASDKAHECLSLGFKVGLGILSLSAAHSSCSRFLENHGLSTLSHYHLHKHYTEVVGGHGWNGEFSLMNQDSQGFHKWLESLKDHPDVISYRLRPMYKLVPFRVQRDALKAATDQYLKENSITSSDRRPSCGYSSNHDWNCCPEQTGKGQLKVTLIGAWSLKGDWWGLTEGTSTIRSNNPQWNVEYNLGSVSVHLNFIHKSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFX5</accession>
    <name>A0A673BFX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha</fullName>
            <ecNumber evidence="18">2.7.1.149</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Diphosphoinositide kinase 2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Phosphatidylinositol 5-phosphate 4-kinase type II alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">PtdIns(5)P-4-kinase isoform 2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">pip4k2aa</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:55993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + GTP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:55965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12280"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.149"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:12281"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.149" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A673BFX5"/>
    <dbReference type="Ensembl" id="ENSSORT00005040526.1">
        <property type="protein sequence ID" value="ENSSORP00005039517.1"/>
        <property type="gene ID" value="ENSSORG00005018265.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BFX5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016309">
        <property type="term" value="F:1-phosphatidylinositol-5-phosphate 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 5-phosphate 4-kinase type-2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 5-phosphate 4-kinase type-2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF21">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE TYPE-2 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 24" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="26">
        <location>
            <begin position="32"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="307"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00042005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00042280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="425" mass="48535" checksum="7C3B6160074683DF" modified="2020-06-17" version="1">MASASSIAASIASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPIMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPLVFRNLRERFGIDDQDFLNSLTRSAPLNSEAQGRSGARFHTSYDKRYVIKTISSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGDETYMIVTRNVFSHRLSVYKKYDLKGSTVAREASDKEKAKELPTYKDNDFINDGQKIYIDGENKKMFLEKLRKDVEVCLQQRLQLKTHIFFFRFGFYFLAQLKLMDYSLLVGIHDVERAEQEEVESEDNEAEEEGESDGGGIGTPPDSPSNTLDSTKPLSPGEFDPAIDVYAIKSNDSAPRKEVYFMAVIDILQHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFYDFITTILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJG7</accession>
    <name>A0A673BJG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">epb41l2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BJG7"/>
    <dbReference type="Ensembl" id="ENSSORT00005042972.1">
        <property type="protein sequence ID" value="ENSSORP00005041900.1"/>
        <property type="gene ID" value="ENSSORG00005018114.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJG7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13184">
        <property type="entry name" value="FERM_C_4_1_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17106">
        <property type="entry name" value="FERM_F1_EPB41L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000002">
        <property type="entry name" value="Erythrocyte protein band 4.1-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008379">
        <property type="entry name" value="Band_4.1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007477">
        <property type="entry name" value="SAB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF20">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05902">
        <property type="entry name" value="4_1_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04382">
        <property type="entry name" value="SAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002304">
        <property type="entry name" value="Membrane_skeletal_4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="7">
        <location>
            <begin position="215"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="661"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="705"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="671"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="714"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="774"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="794"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="811"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="854"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1032" mass="115105" checksum="116A3F92A532C00C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMU7</accession>
    <name>A0A673BMU7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gpr180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029986270.1">
        <property type="nucleotide sequence ID" value="XM_030130410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BMU7"/>
    <dbReference type="Ensembl" id="ENSSORT00005045180.1">
        <property type="protein sequence ID" value="ENSSORP00005044061.1"/>
        <property type="gene ID" value="ENSSORG00005020312.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416591"/>
    <dbReference type="CTD" id="160897"/>
    <dbReference type="InParanoid" id="A0A673BMU7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053880">
        <property type="entry name" value="GPR180-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047831">
        <property type="entry name" value="GPR180/TMEM145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019336">
        <property type="entry name" value="GPR180/TMEM145_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252:SF29">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252">
        <property type="entry name" value="INTIMAL THICKNESS RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21870">
        <property type="entry name" value="GP180_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10192">
        <property type="entry name" value="GPR180-TMEM145_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025445071" evidence="7">
        <location>
            <begin position="21"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180-like N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180/TMEM145 transmembrane" evidence="8">
        <location>
            <begin position="171"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="428" mass="47985" checksum="CE7917A20FAD617E" modified="2020-06-17" version="1">MPHLVTTLVAVVWFSPEVCGKTVTGLFKSDEARQQNGQFITQFMFQGDSGLLVVRVDNVAEAVLKGSRLLLYQDMDPDLDHLSCSQRLAGAQFTIELRQDEHNQTIPPLVTPTAWRALYADKYTCEQDAPVLPSRSDLSFTVLLLNPDSAGNPLEHFSAEEAGLLRFYFLLLLAYFITSCVYAQPLYQALRKVGPMHTVLKVLTTALVLQGCSALCNYIHLDRYSRDGVGIPLMGKLAEVLDSVSQVSLLYLLLSLVLGWTLGRGRTKPLHWERTPASTTVTVGGVITQGVLLLWDQFSDRDSPLSPQTLASVLVLVFRVVLVLLMSSVLYQSMSTERSRLKRDFYWSFSKGCFLWFLSHPVLVLLSVVFNEHQRHKVVTVGGILCQSISMVILYQLFLSRSLYWEVSSLSSVSLPLTMSRTNHRGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BPR6</accession>
    <name>A0A673BPR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lysophospholipase</fullName>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">pnpla7b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40808"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-sn-glycero-3-phosphate + H2O = sn-glycerol 3-phosphate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49092"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:49093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673BPR6"/>
    <dbReference type="Ensembl" id="ENSSORT00005044308.1">
        <property type="protein sequence ID" value="ENSSORP00005043214.1"/>
        <property type="gene ID" value="ENSSORG00005014514.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BPR6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07225">
        <property type="entry name" value="Pat_PNPLA6_PNPLA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000012">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000001">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000022">
        <property type="entry name" value="Patatin like phospholipase domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF23">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 17" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025661558" description="lysophospholipase" evidence="18">
        <location>
            <begin position="22"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="129"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="388"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="523"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="20">
        <location>
            <begin position="858"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="17">
        <location>
            <begin position="862"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="17">
        <location>
            <begin position="889"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="17">
        <location>
            <begin position="1011"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="891"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1243" mass="138925" checksum="23A450459FC4A162" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSZ9</accession>
    <name>A0A673BSZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ras-related protein Rab-19</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115430741</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030006791.1">
        <property type="nucleotide sequence ID" value="XM_030150931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BSZ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005047106.1">
        <property type="protein sequence ID" value="ENSSORP00005045951.1"/>
        <property type="gene ID" value="ENSSORG00005021070.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430741"/>
    <dbReference type="InParanoid" id="A0A673BSZ9"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01864">
        <property type="entry name" value="Rab19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000887">
        <property type="entry name" value="Ras-related protein Rab-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048040">
        <property type="entry name" value="Rab19/43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="224" mass="25069" checksum="2DBCAC7A31FAC64D" modified="2020-06-17" version="1">MMQSPGPEHDDSFDFLFKIILIGDSNVGKTCVVQNFKSGAFSAKQQNTIGVDFTVRTMDIEGKKVKMQVWDTAGQERFRTITQSYYRSAHGAMIAYDITRRSTFDSVTHWIKEVELYGAANVVMALIGNKCDLEDERHVRFQEACALANEKGLIAALETSAKDSQNVEEAFMMMARELLSRNGLHVQQEDVKSSSTPRILLRANSRPVDITSAAYTPPEKKSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUR5</accession>
    <name>A0A673BUR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Laminin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BUR5"/>
    <dbReference type="Ensembl" id="ENSSORT00005046003.1">
        <property type="protein sequence ID" value="ENSSORP00005044869.1"/>
        <property type="gene ID" value="ENSSORG00005020630.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUR5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Laminin N-terminal" evidence="3">
        <location>
            <begin position="38"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="174" mass="19582" checksum="8AEC8ADD56F577ED" modified="2020-06-17" version="1">TPFDTQTLSSRLHDISEDGAMWVGISSNGSNQFIGRMQDFRFYPATLTNREIVEVYSGILPQIHSQSECRCPSSHPRVHPLIERYCIPNAVEDTTNDRVLRLNQDAHPLSYVNDQDMGTTWLSKIMTTQELDEGIAITMDLANGQYQSYNVHAVVALVSMPLLSNNRVITIVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BX97</accession>
    <name>A0A673BX97_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Olfactomedin-like protein 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">olfml2bb</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BX97"/>
    <dbReference type="Ensembl" id="ENSSORT00005047564.1">
        <property type="protein sequence ID" value="ENSSORP00005046405.1"/>
        <property type="gene ID" value="ENSSORG00005021245.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="8626508at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF79">
        <property type="entry name" value="OLFACTOMEDIN-LIKE PROTEIN 2B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025564473" evidence="5">
        <location>
            <begin position="20"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="6">
        <location>
            <begin position="476"/>
            <end position="728"/>
        </location>
    </feature>
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        <location>
            <begin position="200"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="380"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="421"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
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        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="728" mass="81622" checksum="E4AE8BD2DC5BA454" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZ57</accession>
    <name>A0A673BZ57_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSSORG00005021522.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="183" mass="20820" checksum="5899C7586AB7E545" modified="2020-06-17" version="1">MIPSIYVPQYLCSPISMFPSIYVPQYLCSPVSVFPSIYDPQYLCSPVSVFPNIYVPQYLCSPVSVFPSIYDPQYLCSPVSMFPSIYVPQYLCSLVFMIPSIYVPQYLCSLVFMIPSIYVPQYLCSLVFMIPISVPQYLCSPVSMFPSIYVPQYLCSPVSMFPSIYDPQYLCSPVSMFPSIYVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0Q2</accession>
    <name>A0A673C0Q2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable RNA-binding protein EIF1AD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 1A domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">eif1ad</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EIF1AD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002073.1">
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    <dbReference type="FunCoup" id="A0A673C0Q2">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047118.1">
        <property type="protein sequence ID" value="ENSSORP00005045963.1"/>
        <property type="gene ID" value="ENSSORG00005021077.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="1738325at2759"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21641:SF0">
        <property type="entry name" value="RNA-BINDING PROTEIN EIF1AD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21641">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
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        <location>
            <begin position="105"/>
            <end position="184"/>
        </location>
    </feature>
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        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007340"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="184" mass="21626" checksum="284D742689059DB4" modified="2020-06-17" version="1">MSQATKRKHVVKEVLGDFVTPTENQQIVKVIGSRGNNLHETVTAQGDTFLVSMPTKFRKNIWIKRGDYVIVDPIEEGEKVKAEISFILYKDHIQYLQKQQLWPEGFKEESSQDKTQKEHEKDEEKDEEEGSDSEDDESDLFVNTNRRDYQYSESEEEEDSEEEDDDDDHNNDDDDDDEEERTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1A3</accession>
    <name>A0A673C1A3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription initiation factor TFIID subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">TBP-associated factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">taf3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TAF3 forms the TFIID-A module together with TAF5 and TBP. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The TAF3-TBPL2 complex replaces TFIID at specific promoters at an early stage in the differentiation process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TAF3 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030137029.1"/>
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    <dbReference type="FunCoup" id="A0A673C1A3">
        <property type="interactions" value="1093"/>
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        <property type="protein sequence ID" value="ENSSORP00005046163.1"/>
        <property type="gene ID" value="ENSSORG00005021156.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421243"/>
    <dbReference type="CTD" id="83860"/>
    <dbReference type="InParanoid" id="A0A673C1A3"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:transcription factor TFIID complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:p53 binding"/>
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        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="term" value="F:transcription regulator inhibitor activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of signal transduction by p53 class mediator"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000122">
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006366">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="HFD_TAF3"/>
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        <property type="entry name" value="PHD_TAF3"/>
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        <property type="entry name" value="Histone, subunit A"/>
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        <property type="entry name" value="BTP"/>
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    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
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    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Bromo_TP"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="873"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="159"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="401"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="468"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="778"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="159"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="173"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="305"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="319"/>
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    </feature>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="402"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="616"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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    </feature>
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            <begin position="804"/>
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        </location>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057922"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060873"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068909"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076309"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="937" mass="104881" checksum="8EDE90533101BD0B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6L2</accession>
    <name>A0A673C6L2_9TELE</name>
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        <recommendedName>
            <fullName evidence="11">RabBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RabBD" evidence="8">
        <location>
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        </location>
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        <location>
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    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26854" checksum="AAE71A75AEE77BEB" modified="2020-06-17" version="1">MCLADRGNWTMQPGPGPGPDLTDEEKEIINSVIARAEKMEAMEQERIGRLINRLDDMKKTVCGDGVSRCLVCGEQLGSPGVSSVVCEDCKKSMCTKCGTQCSSRPRAVWLCKICREQREVWKRSGAWFFKGFPKHFLPSPMPLSKPKETSSWCNSVPLRSEQVINIYKMHLLNYQWLREIKISLQCCPFLFQNTTPSVSLDQHLNSRPSATPLLIALSPNITPFICLPNLAVPPTVGRGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7N1</accession>
    <name>A0A673C7N1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">eef1a1a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C7N1"/>
    <dbReference type="Ensembl" id="ENSSORT00005050453.1">
        <property type="protein sequence ID" value="ENSSORP00005049254.1"/>
        <property type="gene ID" value="ENSSORG00005022349.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C7N1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000090">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000168">
        <property type="entry name" value="Elongation factor 1-alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="487" mass="53251" checksum="962406DE7415A6FF" modified="2020-06-17" version="1">MGKEKLHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGINKMDSTEPNYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSPNMTWFKGWKINRKEGNASGTTLLEALDAIQPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILKPGMVVTFAPVNVTTEVKSVEMHHEALTEALPGDNVGFNVKNVSVKDIRRGNVAGDSKNDPPQEAANFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVDMIPGKPMCVESFSEYPPLGKCDDSGLKKSFCCARHASDRSSRRHQGLPRRPRGTNECCATLPTLRSPKAGHQLSTSIPRLAVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C984</accession>
    <name>A0A673C984_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine rich repeat transmembrane neuronal 4 like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C984"/>
    <dbReference type="Ensembl" id="ENSSORT00005050548.1">
        <property type="protein sequence ID" value="ENSSORP00005049347.1"/>
        <property type="gene ID" value="ENSSORG00005022416.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C984"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000082">
        <property type="entry name" value="Leucine-rich repeat-containing 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF120">
        <property type="entry name" value="LEUCINE-RICH REPEAT TRANSMEMBRANE NEURONAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025665231" evidence="5">
        <location>
            <begin position="27"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="506" mass="57339" checksum="1B7FC38309B36E08" modified="2020-06-17" version="1">MSDGRLAHLLFPLLLLLRAPLLYSFGERTCPNNCRCEGKTVHCDSSGLLDVPENISVGCQGLSLRYNDLHTLLPYQFAHLSQLLWIYLDHNQISAVDSRAFQGVRRLKELILSSNRITSLHNSTFHGIPNLRSLDLSYNKLEILQPGQFHGLRKLQNLHLRSNGLSNIPIRAFLECRSLEFLDLGYNRIKALTRTTFLGLQKLMELHLEHNQFSRINFFLFPRLANLRSLYLQWNRIRVVNQGLPWTWYTLQKLDLSGNEIQILDPAVFHCLPNLQVLNLESNKLSNVSQEAVSAWISLTSISLAGNMWDCGPGICPLVAWLRNFRGSKDTTMICSSPKYLQGEKIMEVTRNHGICEETDYVLTETPSPMSELISEATAEPTFGPTSDSVSRARPTHPPPPEIEHMTLHKVVVGSVALFFTMSLILTIIYVLWRRYPSATRLLQQRSMVGRKRRKKSPEPEQDLSSQLQEYYMSYNPAATPEALEVLGNGTGSCTCTISGSRECEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9K4</accession>
    <name>A0A673C9K4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 23">Protein kinase C</fullName>
            <ecNumber evidence="5 23">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">prkcab</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="27">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="27">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="5 23"/>
    <dbReference type="AlphaFoldDB" id="A0A673C9K4"/>
    <dbReference type="FunCoup" id="A0A673C9K4">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051147.1">
        <property type="protein sequence ID" value="ENSSORP00005049944.1"/>
        <property type="gene ID" value="ENSSORG00005022661.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C9K4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20833">
        <property type="entry name" value="C1_cPKC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20836">
        <property type="entry name" value="C1_cPKC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04026">
        <property type="entry name" value="C2_PKC_alpha_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000012">
        <property type="entry name" value="Kinase C alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000023">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000080">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000006">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000031">
        <property type="entry name" value="Protein kinase C alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014375">
        <property type="entry name" value="Protein_kinase_C_a/b/g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000550">
        <property type="entry name" value="PKC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="18 23 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17 27" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 27" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 23 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 23" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="31">
        <location>
            <begin position="85"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="31">
        <location>
            <begin position="150"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="29">
        <location>
            <begin position="207"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="30">
        <location>
            <begin position="386"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="32">
        <location>
            <begin position="645"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="24">
        <location>
            <position position="510"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <begin position="392"/>
            <end position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26 28">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRNR" id="PIRNR000550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="719" mass="82294" checksum="2FBEACF43D14C7DF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CC75</accession>
    <name>A0A673CC75_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CWH43-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tmem150c</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DRAM/TMEM150 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004305.1">
        <property type="nucleotide sequence ID" value="XM_030148445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CC75"/>
    <dbReference type="FunCoup" id="A0A673CC75">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052115.1">
        <property type="protein sequence ID" value="ENSSORP00005050899.1"/>
        <property type="gene ID" value="ENSSORG00005023029.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429186"/>
    <dbReference type="CTD" id="441027"/>
    <dbReference type="InParanoid" id="A0A673CC75"/>
    <dbReference type="OrthoDB" id="9865811at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 150C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="7">
        <location>
            <begin position="16"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="257" mass="29189" checksum="9D14ADEB18AFA790" modified="2020-06-17" version="1">MDSGVRGGRMRGISLWALLPPLYSLCVTIGLWVVYFVAVNTGQVVPLTSQYRRNGSARPPYISIAGNSPPASCIFSEVMNLAAFGGFVIALLRYLQLKNRIDKAWQNVTSLVVFSIGSFGMTLIGNFQLFIEEDIHNFGTFLTFGLGTVFCWGQSYLTFKVNLRNEGFKTSMLRFLLSGCITFCLVLYFSLMFQRLHMHAARSQWALVMFFLIFISSFCIELRYNHFEIVCTETNCRHEQKDSDTDLNMTHVQNIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CC89</accession>
    <name>A0A673CC89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Spartin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">spartb</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Lipophagy receptor that plays an important role in lipid droplet (LD) turnover in motor neurons. Localizes to LDs and interacts with components of the autophagy machinery, such as MAP1LC3A/C proteins to deliver LDs to autophagosomes for degradation via lipophagy. Lipid transfer protein required for lipid droplet degradation, including by lipophagy. Can bind and transfer all lipid species found in lipid droplets, from phospholipids to triglycerides and sterol esters but the direction of lipid transfer by spartin and its cargos are unknown. May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with ITCH and WWP1. Interacts (via MIT domain) with IST1; leading to the recruitment of SPART to midbodies. Interacts with MAP1LC3A and MAP1LC3C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CC89"/>
    <dbReference type="Ensembl" id="ENSSORT00005050941.1">
        <property type="protein sequence ID" value="ENSSORP00005049738.1"/>
        <property type="gene ID" value="ENSSORG00005022564.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CC89"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061724">
        <property type="term" value="P:lipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02679">
        <property type="entry name" value="MIT_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000009">
        <property type="entry name" value="spartin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF43">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MIT" evidence="19">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="98"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="564" mass="60078" checksum="9755AC9F6F6DC697" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKJ7</accession>
    <name>A0A673CKJ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine rich repeat transmembrane neuronal 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CKJ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005053841.1">
        <property type="protein sequence ID" value="ENSSORP00005052593.1"/>
        <property type="gene ID" value="ENSSORG00005023695.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKJ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000082">
        <property type="entry name" value="Leucine-rich repeat-containing 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF209">
        <property type="entry name" value="LEUCINE-RICH REPEAT TRANSMEMBRANE NEURONAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025458504" evidence="4">
        <location>
            <begin position="21"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="364" mass="40712" checksum="80E40EBF68ABB686" modified="2020-06-17" version="1">AYCVPLWLVFTVVVPAWLLAAPTGIRERPCPQSCRCDGKIIYCESNAFRDVPSNVSVGTQGLSLRYNSLVNLRSHQFAGLSQLVWLYLDHNYINAVDGQAFHGIRRLKELILSSNKIPLLKNNTFHDVPNLRNLDLSYNKLQVLQPNQFVGLRKLLSLHLRSNSLKTIPMRLDLSGNELQQTVEAWISLTTISLAGNLWYCNLNICPLVAWLKAFKGNKETTMICAGPKEAQGEKVNDVVETYNVCTATPTPIPSTTSARTSVFQPELKPLPTQSVMDKTLIWNRTVSPTPSEASPTIPLPDTEYVSFHKIIAGSVALLLSVAIILLVIYVSWKRYPTIMLLRFYSLVETAEFKQSPQAEGRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CL67</accession>
    <name>A0A673CL67_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Multiple C2 domains, transmembrane 1a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mctp1a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCTP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CL67"/>
    <dbReference type="Ensembl" id="ENSSORT00005056231.1">
        <property type="protein sequence ID" value="ENSSORP00005054955.1"/>
        <property type="gene ID" value="ENSSORG00005024580.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CL67"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04042">
        <property type="entry name" value="C2A_MCTP_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08376">
        <property type="entry name" value="C2B_MCTP_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000050">
        <property type="entry name" value="Multiple C2 and transmembrane domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000287">
        <property type="entry name" value="Multiple C2 domains, transmembrane 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013583">
        <property type="entry name" value="MCTP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911:SF3">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08372">
        <property type="entry name" value="PRT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="684"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="785"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="846"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="877"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="180"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="337"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="73"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="897" mass="101720" checksum="CEC2516FF8045044" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CUR8</accession>
    <name>A0A673CUR8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CUR8"/>
    <dbReference type="FunCoup" id="A0A673CUR8">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059468.1">
        <property type="protein sequence ID" value="ENSSORP00005058145.1"/>
        <property type="gene ID" value="ENSSORG00005025689.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CUR8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005127">
        <property type="term" value="F:ciliary neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070120">
        <property type="term" value="P:ciliary neurotrophic factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000151">
        <property type="entry name" value="Ciliary_neurotrophic_fac_CNTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15196">
        <property type="entry name" value="CILIARY NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15196:SF1">
        <property type="entry name" value="CILIARY NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="195" mass="21986" checksum="1172437309CA645A" modified="2020-06-17" version="1">MAEQEEQSRTAADMEPPVPGQSRTGRAVALARLLHHECTHLLQLYKEKETLLVGLTPEGGRIVCLSPDTQEPGPEGQVQRLHSALRQCLGLLHCVILKEEEEWGELEGDYESMRKNVRQRLEHLLVSTKDLVETEDVTLEVTPDHQCNEETDGAGGIFALKMWTYRVLEELIHWADHAAQTLHALHTEREGTADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CYU7</accession>
    <name>A0A673CYU7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dipeptidyl peptidase 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fap</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Invadopodium membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CYU7"/>
    <dbReference type="FunCoup" id="A0A673CYU7">
        <property type="interactions" value="282"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059405.1">
        <property type="protein sequence ID" value="ENSSORP00005058082.1"/>
        <property type="gene ID" value="ENSSORG00005025625.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CYU7"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:proteolysis"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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        <property type="entry name" value="DPPIV_rep"/>
    </dbReference>
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        <property type="entry name" value="Pept_S9_AS"/>
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    </dbReference>
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        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF204">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 4"/>
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    </dbReference>
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        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_S9"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="DPP6 N-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="PRO_ENDOPEP_SER"/>
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        <location>
            <begin position="6"/>
            <end position="27"/>
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        <location>
            <begin position="33"/>
            <end position="52"/>
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    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="8">
        <location>
            <begin position="101"/>
            <end position="458"/>
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    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="7">
        <location>
            <begin position="542"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004485"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18811"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="748" mass="85570" checksum="2D8857C8376A024D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y304</accession>
    <name>A0A672Y304_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Neoteleostei</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="21"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="102" mass="11513" checksum="AEB467D4089C8E6A" modified="2020-06-17" version="1">AFWSVHGQTLTQSEPAVKRPGQSHRLTCTTSGFMFSSYWMAWIRQAPGKGLEWIAVDNSGGFNSYSQSVQGRFTTSRDNSRNQLYLQMNSLKTEDSAVYYCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5Q3</accession>
    <name>A0A672Y5Q3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GOST seven transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y5Q3"/>
    <dbReference type="Ensembl" id="ENSSORT00005001907.1">
        <property type="protein sequence ID" value="ENSSORP00005001852.1"/>
        <property type="gene ID" value="ENSSORG00005001163.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF52">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 87A PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="7">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="132" mass="14833" checksum="6A2CE1D1973A709D" modified="2020-06-17" version="1">MVMCIVYVLMAVLWLVLSACYWRDLLRIQFWIGGVIFLGMVEKAMYYAEFQNIRYHGHSVPGAVVSAEVVSAVKRTLARVLVIIASLGYGIVKPRLGALLHRVVGVGLLYLIFSLIEGLLRIMFMFVCIPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y939</accession>
    <name>A0A672Y939_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y939"/>
    <dbReference type="Ensembl" id="ENSSORT00005003427.1">
        <property type="protein sequence ID" value="ENSSORP00005003328.1"/>
        <property type="gene ID" value="ENSSORG00005002030.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y939"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YI01"/>
    <dbReference type="FunCoup" id="A0A672YI01">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005004291.1">
        <property type="protein sequence ID" value="ENSSORP00005004168.1"/>
        <property type="gene ID" value="ENSSORG00005002520.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YI01"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902711">
        <property type="term" value="C:GABA-A receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:chloride channel activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19053">
        <property type="entry name" value="LGIC_TM_GABAAR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta-2 isoform A"/>
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        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta-2 isoform A"/>
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        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
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        <property type="entry name" value="GABAAb_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01160">
        <property type="entry name" value="GABAARBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="94"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="306"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="532" mass="60029" checksum="7D7CAFBB35B356BD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJM8</accession>
    <name>A0A672YJM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">klhl41a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Sarcoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YJM8"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005004912.1">
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        <property type="gene ID" value="ENSSORG00005002889.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJM8"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000006">
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    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000025">
        <property type="entry name" value="Kelch-like family member 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="BACK"/>
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    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
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    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF146">
        <property type="entry name" value="KELCH-LIKE PROTEIN 41"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="32"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="600" mass="68057" checksum="526E656E7E21BEDE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLQ0</accession>
    <name>A0A672YLQ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Somatostatin receptor 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLQ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005005735.1">
        <property type="protein sequence ID" value="ENSSORP00005005541.1"/>
        <property type="gene ID" value="ENSSORG00005003313.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLQ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004994">
        <property type="term" value="F:somatostatin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071385">
        <property type="term" value="P:cellular response to glucocorticoid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000060">
        <property type="entry name" value="Somatostatin receptor type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000586">
        <property type="entry name" value="Somatstn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF35">
        <property type="entry name" value="SOMATOSTATIN RECEPTOR TYPE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00246">
        <property type="entry name" value="SOMATOSTATNR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="49"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="365" mass="40979" checksum="07E321D72C5C3BDF" modified="2020-06-17" version="1">ENEMDYGSYPTGLPYNTSIDYDDYYQEPDASKIIIPSIYALVCCVGLTGNAMVIYVILKYAKMKTATNIYILNLAIADELFMLSVPFLATSAAVRHWPFGSLMCRLVLSVDGINMFTSIFCLTVLSVDRYVAVVHPIKAARYRRPTVAKVVNVCVWGLSLIVILPIIIFADTVPAQDGGVDCNFLWPEAAWSEAFVVYTFLLGFLLPVGAICLCYCLMVARMRAVGLKAGWLQRRRSEKKITRMVLLVVAVFVLCWMPFYIVQLVSVFHRPPDPMVTQLFVILSYANSGANPILYGFVSDNFRRSFQRIVCFRWLESGLDAEQVDYCAVAVKRQATCGPLEFPKDCMASDMVFRNGTYTSRTTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLW3</accession>
    <name>A0A672YLW3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protocadherin 2 alpha a 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLW3"/>
    <dbReference type="Ensembl" id="ENSSORT00005005848.1">
        <property type="protein sequence ID" value="ENSSORP00005005606.1"/>
        <property type="gene ID" value="ENSSORG00005003396.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLW3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF290">
        <property type="entry name" value="PROTOCADHERIN 2 ALPHA A 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="694"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="29"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="136"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="245"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="353"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="459"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="582"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="787"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="789"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="810" mass="90156" checksum="663665FBA68C110C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNC1</accession>
    <name>A0A672YNC1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amine oxidase</fullName>
            <ecNumber evidence="8">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115423712</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="8">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="7">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="7 8">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="8"/>
    <dbReference type="RefSeq" id="XP_029996570.1">
        <property type="nucleotide sequence ID" value="XM_030140710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YNC1"/>
    <dbReference type="Ensembl" id="ENSSORT00005006247.1">
        <property type="protein sequence ID" value="ENSSORP00005005997.1"/>
        <property type="gene ID" value="ENSSORG00005003578.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423712"/>
    <dbReference type="InParanoid" id="A0A672YNC1"/>
    <dbReference type="OrthoDB" id="5379943at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000002">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000018">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000022">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049947">
        <property type="entry name" value="Cu_Am_Ox_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF4">
        <property type="entry name" value="RETINA-SPECIFIC COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00766">
        <property type="entry name" value="CUDAOXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01165">
        <property type="entry name" value="COPPER_AMINE_OXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0801">TPQ</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="11">
        <location>
            <begin position="76"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="12">
        <location>
            <begin position="170"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="10">
        <location>
            <begin position="307"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="6">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="7">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="760" mass="87249" checksum="2FB30F877B6D69E1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNF8</accession>
    <name>A0A672YNF8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Toll-like receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">tlr1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YNF8"/>
    <dbReference type="Ensembl" id="ENSSORT00005006370.1">
        <property type="protein sequence ID" value="ENSSORP00005006119.1"/>
        <property type="gene ID" value="ENSSORG00005003636.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YNF8"/>
    <dbReference type="OrthoDB" id="1081807at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000001">
        <property type="entry name" value="Toll-like receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017241">
        <property type="entry name" value="Toll-like_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF422">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037595">
        <property type="entry name" value="Toll-like_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0391">Immunity</keyword>
    <keyword evidence="13 14" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3 14" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025341826" evidence="16">
        <location>
            <begin position="23"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="17">
        <location>
            <begin position="643"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="421"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR037595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037595-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="800" mass="91038" checksum="3AB2C294D6116638" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNL6</accession>
    <name>A0A672YNL6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Paladin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pald1a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the paladin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YNL6"/>
    <dbReference type="FunCoup" id="A0A672YNL6">
        <property type="interactions" value="179"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006348.1">
        <property type="protein sequence ID" value="ENSSORP00005006097.1"/>
        <property type="gene ID" value="ENSSORG00005003624.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YNL6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17660">
        <property type="entry name" value="PTP_paladin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000100">
        <property type="entry name" value="Phosphatase domain-containing paladin 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14566">
        <property type="entry name" value="PTPlike_phytase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01301">
        <property type="entry name" value="PTPlike_phytase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="867" mass="99363" checksum="4A88F100E4B97851" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNP2</accession>
    <name>A0A672YNP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small acidic protein-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">knop1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029991049.1">
        <property type="nucleotide sequence ID" value="XM_030135189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YNP2"/>
    <dbReference type="Ensembl" id="ENSSORT00005006373.1">
        <property type="protein sequence ID" value="ENSSORP00005006122.1"/>
        <property type="gene ID" value="ENSSORG00005003638.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420035"/>
    <dbReference type="CTD" id="400506"/>
    <dbReference type="InParanoid" id="A0A672YNP2"/>
    <dbReference type="OrthoDB" id="9451331at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028124">
        <property type="entry name" value="SMAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22426">
        <property type="entry name" value="ARGININE_SERINE-RICH COILED-COIL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22426:SF1">
        <property type="entry name" value="LYSINE-RICH NUCLEOLAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15477">
        <property type="entry name" value="SMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small acidic protein-like" evidence="2">
        <location>
            <begin position="453"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="549" mass="62314" checksum="90265332370B7CA7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YP91</accession>
    <name>A0A672YP91_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Programmed cell death protein 10 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pdcd10a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PDCD10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YP91"/>
    <dbReference type="Ensembl" id="ENSSORT00005006646.1">
        <property type="protein sequence ID" value="ENSSORP00005006390.1"/>
        <property type="gene ID" value="ENSSORG00005003767.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YP91"/>
    <dbReference type="OrthoDB" id="6017654at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090443">
        <property type="term" value="C:FAR/SIN/STRIPAK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903358">
        <property type="term" value="P:regulation of Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009652">
        <property type="entry name" value="PDCD10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048288">
        <property type="entry name" value="PDCD10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13250:SF1">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13250">
        <property type="entry name" value="TF-1 CELL APOPTOSIS RELATED PROTEIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06840">
        <property type="entry name" value="PDC10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20929">
        <property type="entry name" value="PDCD10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 10 dimerisation" evidence="11">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="210" mass="24292" checksum="7C2F92240F5044CC" modified="2020-06-17" version="1">MLMEVMKNEADRTSVASMALYSVMYPVFNQLKRVDLSAAETLKTAFIKAEKENPGLTQDIVVKILEKKRLEINYNECLLRMAADDVEEFMIDRPELEFQELNERSRALKHILSTIPDDISDKVGFLQTIKDIASAIKELLDTVNTVFRKYQHQNRRALEQQKKEFVKYSKSFSDTLKTYFKDGKVINVFVSANRLTHQTNMILLAFKTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YPL3</accession>
    <name>A0A672YPL3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translocase of outer mitochondrial membrane 6 homolog (yeast)</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YPL3"/>
    <dbReference type="FunCoup" id="A0A672YPL3">
        <property type="interactions" value="743"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006793.1">
        <property type="protein sequence ID" value="ENSSORP00005006536.1"/>
        <property type="gene ID" value="ENSSORG00005003832.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YPL3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029182">
        <property type="entry name" value="TOMM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15527">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15527:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15184">
        <property type="entry name" value="TOM6p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="66" mass="7133" checksum="EFEA3A969B1A40D0" modified="2020-06-17" version="1">MSASNGKKGSSPSSGVVEWISSACRFATDRNDFRRNLLVNLGLFAAGVWVARNLSDFDLMSPQPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQR5</accession>
    <name>A0A672YQR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anamorsin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytokine-induced apoptosis inhibitor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Fe-S cluster assembly protein DRE2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CIAPIN1</name>
        <name evidence="15" type="synonym">LOC115416664</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the scaffold complex NUBP1-NUBP2. Electrons are transferred to CIAPIN1 from NADPH via the FAD- and FMN-containing protein NDOR1. NDOR1-CIAPIN1 are also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit. Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. Interacts with NDOR1. Interacts with CHCHD4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">The twin Cx2C motifs are involved in the recognition by the mitochondrial CHCHD4/MIA40-GFER/ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the anamorsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YQR5"/>
    <dbReference type="FunCoup" id="A0A672YQR5">
        <property type="interactions" value="1545"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007129.1">
        <property type="protein sequence ID" value="ENSSORP00005006867.1"/>
        <property type="gene ID" value="ENSSORG00005003982.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YQR5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000085">
        <property type="entry name" value="Anamorsin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03115">
        <property type="entry name" value="Anamorsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049011">
        <property type="entry name" value="Anamorsin_N_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20922">
        <property type="entry name" value="Anamorsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anamorsin N-terminal" evidence="14">
        <location>
            <begin position="9"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Anamorsin C-terminal" evidence="13">
        <location>
            <begin position="258"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fe-S binding site B" evidence="11">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 1" evidence="11">
        <location>
            <begin position="261"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 2" evidence="11">
        <location>
            <begin position="272"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="299" mass="31912" checksum="8F2C8B002D46C477" modified="2020-06-17" version="1">MADLDIKAGDQVLLVWTQPSAPEALKDLAEELGSAVGANGKVSVENMDRLLLCDRLSSHAASTFDRVLSCLLADSSSIHSSETLAEIARVLKPGGTLVLDEPVRTAEKLISALKLSGYTSVKEVRKTELSPEALTSLRAATGYHGNTLSRIRVSASKPNFEVGSSSQIKLSFGKKTPKPAEKPALDPNTVKMWTLSATDMNDDDVDLLDSDALLDEDDLKKPDPSSLKAPGCGDGAGKKKKACKNCRTVKANRRELPKSACGSCYLGDAFRCASCPYLGMPAFKPGEKIKLDDKTLTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YR63</accession>
    <name>A0A672YR63_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">psmb13a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="8">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YR63"/>
    <dbReference type="Ensembl" id="ENSSORT00005007337.1">
        <property type="protein sequence ID" value="ENSSORP00005007074.1"/>
        <property type="gene ID" value="ENSSORG00005004073.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YR63"/>
    <dbReference type="OrthoDB" id="429533at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024689">
        <property type="entry name" value="Proteasome_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF9">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12465">
        <property type="entry name" value="Pr_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <feature type="domain" description="Proteasome beta subunit C-terminal" evidence="11">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="274" mass="29708" checksum="0E90F80A492D9FA6" modified="2020-06-17" version="1">MALSNILETPAAGFNFDNVVRNAALEGLLEEQPPKPLKTGTTIAGLIFKDGVVLGADTRATSSEVVADKMCAKIHYIAPNIYCCGAGTAADTEKTTELLSSNLTIFSLNSGRNPRMVMAVNILQDMLYRYHGNIGANLILGGVDCTGNHLYTVGPWGSVNKVPYLAMGSGDLAALGILEDRFKHDLELEEAKELVRAAIHAGIMCDLGSGNNIDICVITRKGVDYIRPYQESAYKDTRKIKYKYRPGTTPVLTQKVVPLKLEVVQETVQQMDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YW61</accession>
    <name>A0A672YW61_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YW61"/>
    <dbReference type="Ensembl" id="ENSSORT00005009100.1">
        <property type="protein sequence ID" value="ENSSORP00005008799.1"/>
        <property type="gene ID" value="ENSSORG00005004851.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YW61"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053328">
        <property type="entry name" value="Uro-adherence_factor_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34592">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34592:SF2">
        <property type="entry name" value="SPT5 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="252" mass="26245" checksum="5B132931A7B3E0DA" modified="2020-06-17" version="1">MISPPIFRPGLRPGLKSGVRPGLRPGLRHGLRPGLRPGLKSGLRPRLNLGLRPGLRPGLKSGLRPGLNLGLRPGLRPGLKSGLRPGLNLGLRPGLRPGLKSGLRPGLNLGLRPGLKSGLRPGLNLGSRPGLRPGLNLGLRPGLNLGLRPGLRLGLKSGLRPGLNLGLRPGLNLGSRPGLRPGLNLGLRPGLRLGLKSGLRPGLNLGLRPGLKSGLRPGLNLGSRPGLRPGLKSGLRPGLRCGFRFLIILSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YW68</accession>
    <name>A0A672YW68_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YW68"/>
    <dbReference type="Ensembl" id="ENSSORT00005008982.1">
        <property type="protein sequence ID" value="ENSSORP00005008682.1"/>
        <property type="gene ID" value="ENSSORG00005004790.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YW68"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="18"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="130" mass="14249" checksum="B80A3EBB73A19794" modified="2020-06-17" version="1">MDVCLKLVWSLCFSESRGQVTVTQPGAVSSDLGASATITCTTSQDVYVYSSNHRLAWYQQRDGETPKLLIYWATRRESGISSRFSGSGSNSFFTLTISDVQAEDAAVYYCQSVHYINSQGLHSPSASSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0D4</accession>
    <name>A0A672Z0D4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dpy-19-like 1, like (H. sapiens)</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z0D4"/>
    <dbReference type="Ensembl" id="ENSSORT00005010884.1">
        <property type="protein sequence ID" value="ENSSORP00005010546.1"/>
        <property type="gene ID" value="ENSSORG00005005708.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z0D4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018732">
        <property type="entry name" value="Dpy-19/Dpy-19-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31488:SF1">
        <property type="entry name" value="C-MANNOSYLTRANSFERASE DPY19L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31488">
        <property type="entry name" value="DPY-19-LIKE 1, LIKE (H. SAPIENS)"/>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4Y4</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <begin position="6"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="116" mass="13103" checksum="1FB67ECFBA09E46F" modified="2020-06-17" version="1">IHWRVQDLTSAESVPPASSARALDEPNIPPQNKYSGNPETCRNFFSQIQLIIEAQPRRFSSDSAKIAYVASLLEGPPLSYFNALLEKESPVARSYSRLCAELKRVYDHPWNEKALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6K9</accession>
    <name>A0A672Z6K9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115429305</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z6K9"/>
    <dbReference type="FunCoup" id="A0A672Z6K9">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012501.1">
        <property type="protein sequence ID" value="ENSSORP00005012107.1"/>
        <property type="gene ID" value="ENSSORG00005006415.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z6K9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000336">
        <property type="entry name" value="Si:dkey-222h21.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="128"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="13">
        <location>
            <begin position="271"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="30"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1069" mass="121652" checksum="A76900AD3E332266" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7Q9</accession>
    <name>A0A672Z7Q9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FERM, ARHGEF and pleckstrin domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">FERM, RhoGEF and pleckstrin domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">farp2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Functions as a guanine nucleotide exchange factor that activates RAC1. May have relatively low activity. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Plays a role in TNFSF11-mediated osteoclast differentiation, especially in podosome rearrangement and reorganization of the actin cytoskeleton. Regulates the activation of ITGB3, integrin signaling and cell adhesion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding. Interacts with RAC1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029988228.1">
        <property type="nucleotide sequence ID" value="XM_030132368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z7Q9"/>
    <dbReference type="Ensembl" id="ENSSORT00005012909.1">
        <property type="protein sequence ID" value="ENSSORP00005012512.1"/>
        <property type="gene ID" value="ENSSORG00005004547.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418056"/>
    <dbReference type="CTD" id="9855"/>
    <dbReference type="InParanoid" id="A0A672Z7Q9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13193">
        <property type="entry name" value="FERM_C_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01220">
        <property type="entry name" value="PH1_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13235">
        <property type="entry name" value="PH2_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000040">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000005">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000046">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000020">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041788">
        <property type="entry name" value="FARP1/FARP2/FRMD7_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051835">
        <property type="entry name" value="RAC1-GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858">
        <property type="entry name" value="FERM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858:SF4">
        <property type="entry name" value="FERM, ARHGEF AND PLECKSTRIN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="FERM" evidence="12">
        <location>
            <begin position="50"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="11">
        <location>
            <begin position="553"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="773"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="947"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="425"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="885"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="655"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="435"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="898"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1064" mass="122256" checksum="EE4140E1AF70370F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCC4</accession>
    <name>A0A672ZCC4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent zinc metalloprotease YME1L1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ATP-dependent metalloprotease FtsH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">YME1-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">yme1l1a</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homohexamer; may also form heterohexamers. Exists in several complexes of 600-1100 kDa. Interacts with AFG1L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZCC4"/>
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        <property type="protein sequence ID" value="ENSSORP00005014157.1"/>
        <property type="gene ID" value="ENSSORG00005005682.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZCC4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010636">
        <property type="term" value="P:positive regulation of mitochondrial fusion"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000002">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000195">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="21">
        <location>
            <begin position="324"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00072035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="725" mass="80450" checksum="AE2C284F32DE9DC0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCI8</accession>
    <name>A0A672ZCI8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pre-mRNA-processing-splicing factor 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Splicing factor Prp8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">prpf8</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030007551.1">
        <property type="nucleotide sequence ID" value="XM_030151691.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014994.1">
        <property type="protein sequence ID" value="ENSSORP00005014569.1"/>
        <property type="gene ID" value="ENSSORG00005007442.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431374"/>
    <dbReference type="CTD" id="10594"/>
    <dbReference type="InParanoid" id="A0A672ZCI8"/>
    <dbReference type="OrthoDB" id="1931567at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08056">
        <property type="entry name" value="MPN_PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13838">
        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.230:FF:000003">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000002">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1570.40:FF:000001">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.40">
        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012591">
        <property type="entry name" value="PRO8NT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012592">
        <property type="entry name" value="PROCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012984">
        <property type="entry name" value="PROCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027652">
        <property type="entry name" value="PRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021983">
        <property type="entry name" value="PRP8_domainIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043173">
        <property type="entry name" value="Prp8_domainIV_fingers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043172">
        <property type="entry name" value="Prp8_domainIV_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019581">
        <property type="entry name" value="Prp8_U5-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042516">
        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019580">
        <property type="entry name" value="Prp8_U6-snRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019582">
        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11140:SF0">
        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08082">
        <property type="entry name" value="PRO8NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08083">
        <property type="entry name" value="PROCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08084">
        <property type="entry name" value="PROCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12134">
        <property type="entry name" value="PRP8_domainIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10598">
        <property type="entry name" value="RRM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10597">
        <property type="entry name" value="U5_2-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10596">
        <property type="entry name" value="U6-snRNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MPN" evidence="11">
        <location>
            <begin position="2106"/>
            <end position="2237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2338" mass="273899" checksum="B52EA74239B7A688" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDG1</accession>
    <name>A0A672ZDG1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ataxin 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATXN1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDG1"/>
    <dbReference type="Ensembl" id="ENSSORT00005014943.1">
        <property type="protein sequence ID" value="ENSSORP00005014518.1"/>
        <property type="gene ID" value="ENSSORG00005007429.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDG1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020997">
        <property type="entry name" value="Ataxin-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043404">
        <property type="entry name" value="ATAXIN1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003652">
        <property type="entry name" value="Ataxin_AXH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036096">
        <property type="entry name" value="Ataxin_AXH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13392">
        <property type="entry name" value="ATAXIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13392:SF5">
        <property type="entry name" value="ATAXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12547">
        <property type="entry name" value="ATXN-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08517">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00536">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102031">
        <property type="entry name" value="AXH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51148">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AXH" evidence="10">
        <location>
            <begin position="540"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="137"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="245"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="337"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="688"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="725"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="822" mass="87327" checksum="FD7182F22872FD92" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZEP7</accession>
    <name>A0A672ZEP7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kruppel like factor 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115434858</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZEP7"/>
    <dbReference type="Ensembl" id="ENSSORT00005015392.1">
        <property type="protein sequence ID" value="ENSSORP00005014958.1"/>
        <property type="gene ID" value="ENSSORG00005007604.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZEP7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000926">
        <property type="entry name" value="Kruppel like factor 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF64">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="150"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="180"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="261" mass="28979" checksum="1B6805BF9B2121A0" modified="2020-06-17" version="1">MRTDSDKESAVTQLDSNGASQKPQPQTSSSRPEEKPKPPQSVCPVPSGFYCQILPLPLSTTNTQQQQAPLFQQQQQPGAPAQVILLGPQVAKAPLMLFVPQPVLPPIYVQRPLVTPSGTKLPAIAPALSRGPSVQRSSPAQPQAPRIRSHVCRHDDCKKTYFKSSHLKAHMRTHTGEKPFRCKWEGCERQFARSDELSRHRRTHTGEKRFACPLCLSRFMRSDHLAKHARRHLTARKTTCWTSGVNHPADHSASTALGQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFT6</accession>
    <name>A0A672ZFT6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Gastrula zinc finger protein XlCGF57.1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115428072</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002755.1">
        <property type="nucleotide sequence ID" value="XM_030146895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZFT6"/>
    <dbReference type="Ensembl" id="ENSSORT00005015844.1">
        <property type="protein sequence ID" value="ENSSORP00005015353.1"/>
        <property type="gene ID" value="ENSSORG00005007795.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428072"/>
    <dbReference type="InParanoid" id="A0A672ZFT6"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001370">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000733">
        <property type="entry name" value="Zinc finger protein 236 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="448"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="469"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="490"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="630" mass="71921" checksum="0097848406AAFF78" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGT9</accession>
    <name>A0A672ZGT9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">pou2f1b</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZGT9"/>
    <dbReference type="Ensembl" id="ENSSORT00005016503.1">
        <property type="protein sequence ID" value="ENSSORP00005016004.1"/>
        <property type="gene ID" value="ENSSORG00005008083.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZGT9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045703">
        <property type="entry name" value="POU2F1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000972">
        <property type="entry name" value="TF_octamer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF47">
        <property type="entry name" value="POU DOMAIN, CLASS 2, TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19536">
        <property type="entry name" value="POU2F1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00029">
        <property type="entry name" value="OCTAMER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="10 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025353685" description="POU domain protein" evidence="14">
        <location>
            <begin position="26"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="POU-specific" evidence="16">
        <location>
            <begin position="279"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="378"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="380"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="730" mass="76034" checksum="029CD6E321D6B5F5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJY9</accession>
    <name>A0A672ZJY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcineurin B homologous protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Tescalcin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">tescb</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the calcineurin regulatory subunit family. CHP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZJY9"/>
    <dbReference type="FunCoup" id="A0A672ZJY9">
        <property type="interactions" value="1129"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017978.1">
        <property type="protein sequence ID" value="ENSSORP00005017460.1"/>
        <property type="gene ID" value="ENSSORG00005008724.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJY9"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052490">
        <property type="entry name" value="CHP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46823">
        <property type="entry name" value="CALCINEURIN B HOMOLOGOUS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="112"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="216" mass="25043" checksum="B3FADA2F751D66D7" modified="2020-06-17" version="1">MGALQSAPAQPEYRDLADKTGFSYEQIGVLHKRFKQLSHNEPTLRRDHFSAIPDLACNPIRAQIIEAFFDRRNYRQNNEGKVEEISFEEFLVVMSHFRPPSMNMTDEQRESIRKEKLRFLFNMHDTDNDGTITLQEYRHVVEELLSRSGALGKETAKSIADAAMLEVASISMGHMEPDEFYEGITFEHFLKLLKGFEIESKMNIRFLNMDTTTLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZK01</accession>
    <name>A0A672ZK01_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Eukaryotic peptide chain release factor GTP-binding subunit ERF3A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gspt1l</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZK01"/>
    <dbReference type="FunCoup" id="A0A672ZK01">
        <property type="interactions" value="1303"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018020.1">
        <property type="protein sequence ID" value="ENSSORP00005017501.1"/>
        <property type="gene ID" value="ENSSORG00005005959.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZK01"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="168"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="584" mass="64649" checksum="F617FBCA87CFAA8C" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZKU7</accession>
    <name>A0A672ZKU7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZKU7"/>
    <dbReference type="Ensembl" id="ENSSORT00005017932.1">
        <property type="protein sequence ID" value="ENSSORP00005017414.1"/>
        <property type="gene ID" value="ENSSORG00005008707.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZKU7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="40443" checksum="6AF5FC94244AE2F5" modified="2020-06-17" version="1">YSCKGEFEHLLKCRDQKLAIAFFVSMYGTFPLICVFPVIIEFFCLLLDAKGHRRALAMKKRRAEQLDWTPQPPVPEFVPRMFFHVNYTGRGTGTRNFVRKWPISHLTGRQVKFVPPGHPEKKIVFVVGDSHLRAMLDEDVAIPELPFCFSFLCVPGGVAADLRMEVRHLTVPWTPDLVCVCAPSNNLTGSRTIGEAAVDFDALLRTVRSRWPKVFVLDFPPRLTIPLDLQELLRNEYHRVAARVPYVAVADHLSLNQLNLWCPDGVHLSDTDGMPILVDLLCDAAFSQLALPPPEPTASTQMPPRPWTPPSPRVAPRVVATGPEPLPRRRHPRGWTVVGQEGKVIIASESLPGCHFECVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZPY5</accession>
    <name>A0A672ZPY5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit MAGT1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Magnesium transporter protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115427386</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the OST3/OST6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZPY5"/>
    <dbReference type="FunCoup" id="A0A672ZPY5">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019450.1">
        <property type="protein sequence ID" value="ENSSORP00005018899.1"/>
        <property type="gene ID" value="ENSSORG00005008459.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZPY5"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015693">
        <property type="term" value="P:magnesium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000009">
        <property type="entry name" value="Tumor suppressor candidate 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692:SF2">
        <property type="entry name" value="MAGNESIUM TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="40122" checksum="877E776586EB9258" modified="2020-06-17" version="1">PSHPDAPITLWKRVSLDSDQTTGSFSIETVSNLYALYQNGGSFRKENMLCVLQTLLSEKVTQMMEWASKRSVIRMNGDKFRRFVKAPPRNYSVVIMFTALQPQRQCGVCRQADEEFQVLANSWRYSSAFTNKVFFASVDFDEGSDVFQMLNMNSAPTFLHFPPKGKPRRTDTYELQVRGFAAEQLARWVADRTDVQIRVIRPPNYAGPLLLGFLLAVIGGLAYLRRHNLEFLFNKNVWAFSALCFVLIMTSGQMWNHIRGPPYAHKNPSTGQVSYIHGSSQAQFVAETHIVLLFNAAVTMGMVLLCEAATSDMDIGKRKIMCVAGIGLVMLFFSWLLSIFRAKYHGYPYSFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQV1</accession>
    <name>A0A672ZQV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myotubularin</fullName>
            <ecNumber evidence="8">3.1.3.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Phosphatidylinositol-3-phosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">mtm1</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:45636"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78994"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1-D-myo-inositol-3-phosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42328"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65221"/>
            <dbReference type="ChEBI" id="CHEBI:78934"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:45632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78911"/>
            <dbReference type="ChEBI" id="CHEBI:85342"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:39019"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="EC" id="3.1.3.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Myofibril</location>
            <location evidence="3">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Late endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.95" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZQV1"/>
    <dbReference type="Ensembl" id="ENSSORT00005019322.1">
        <property type="protein sequence ID" value="ENSSORP00005018773.1"/>
        <property type="gene ID" value="ENSSORG00005007941.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQV1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048311">
        <property type="term" value="P:mitochondrion distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902902">
        <property type="term" value="P:negative regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000038">
        <property type="entry name" value="Myotubularin 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF69">
        <property type="entry name" value="MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="28">
        <location>
            <begin position="161"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="27">
        <location>
            <begin position="340"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="573"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="582"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="24">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <begin position="309"/>
            <end position="310"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <begin position="370"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00016293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="607" mass="69982" checksum="81F947D1AF7451F4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWI5</accession>
    <name>A0A672ZWI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-serine deaminase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-threonine dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115434297</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZWI5"/>
    <dbReference type="Ensembl" id="ENSSORT00005021423.1">
        <property type="protein sequence ID" value="ENSSORP00005020797.1"/>
        <property type="gene ID" value="ENSSORG00005010154.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZWI5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000044">
        <property type="entry name" value="Phenylserine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF19">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="6">
        <location>
            <begin position="133"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="524" mass="59094" checksum="845049B8BFB8CEE1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZWW7</accession>
    <name>A0A672ZWW7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">eif4a3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Involved in craniofacial development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZWW7"/>
    <dbReference type="FunCoup" id="A0A672ZWW7">
        <property type="interactions" value="1723"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005022227.1">
        <property type="protein sequence ID" value="ENSSORP00005021581.1"/>
        <property type="gene ID" value="ENSSORG00005008715.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZWW7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18045">
        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000498">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="73"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="254"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXC3</accession>
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            <fullName evidence="11">BCL2-associated athanogene 6</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Gobiaria</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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    <sequence length="1107" mass="130000" checksum="E675072CED27100F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2P9</accession>
    <name>A0A673A2P9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">P2Y purinoceptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115418973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029989400.1">
        <property type="nucleotide sequence ID" value="XM_030133540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A2P9"/>
    <dbReference type="Ensembl" id="ENSSORT00005024188.1">
        <property type="protein sequence ID" value="ENSSORP00005023509.1"/>
        <property type="gene ID" value="ENSSORG00005011384.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418973"/>
    <dbReference type="InParanoid" id="A0A673A2P9"/>
    <dbReference type="OrthoDB" id="9936719at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027294">
        <property type="entry name" value="NPS_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24244:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24244">
        <property type="entry name" value="NEUROPEPTIDE S RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="33"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="339"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="351" mass="39756" checksum="99F3BEA281B776E7" modified="2020-06-17" version="1">MSGNVSCADIDISFTHAFLPPVFAAVFVVGTAANAWGLTSVCRSWRSIGSINVFMLNLGVADLLYLSTLPFLFYYYSHGSRWTFGQTFCKVTRFCFNLNLYGSIGFLTCISVYRYLGIVHPMRVMGRIHTGHSTALAVLVWVLVFIQILPDMFFDKNDETNPNKTEACFDTTSDRQVDRYQPYSLGWAVTGFAVPLVVILACYGHVVYVLATKANINPLLKQRCLKLVVILVILFSVCFIPYHVFRNINLETRILKQEGVCYPSFRGVYIAHQVGRCLACLNSALNPLIYIVGNDEFLMKLHHIGQRVRVSVVSWRDAVQYRKPPLVDESPRRIPANSKDTKDDNRTRDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4A6</accession>
    <name>A0A673A4A6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A4A6"/>
    <dbReference type="Ensembl" id="ENSSORT00005024141.1">
        <property type="protein sequence ID" value="ENSSORP00005023463.1"/>
        <property type="gene ID" value="ENSSORG00005011358.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4A6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000006">
        <property type="entry name" value="Pleckstrin homology like domain family B member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052212">
        <property type="entry name" value="PH-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156:SF22">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN FAMILY B MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN, FAMILY B, MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="96" mass="11689" checksum="DA05386AEEC6A1BF" modified="2020-06-17" version="1">MVKTWKKRWFLFDMDHRRLAYYTDCDERKLKGVIYFQAIEEVYYDHLRTATSSPRPSLTFCVKTYDRLFFLVANNAVSMRIWMDVIVTATDEHCRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4E6</accession>
    <name>A0A673A4E6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cellular communication network factor 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115420051</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A4E6"/>
    <dbReference type="Ensembl" id="ENSSORT00005024186.1">
        <property type="protein sequence ID" value="ENSSORP00005023507.1"/>
        <property type="gene ID" value="ENSSORG00005011383.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4E6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF22">
        <property type="entry name" value="CCN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="IGFBP N-terminal" evidence="9">
        <location>
            <begin position="24"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="8">
        <location>
            <begin position="101"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="7">
        <location>
            <begin position="239"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="37034" checksum="7FBB07201D266649" modified="2020-06-17" version="1">MWSTPWLRRFRSSADIVLSCVQVSSQPCSDPCRCPPVVLRCPPGVLPVLDGCRCCQVCARQEGESCSAHLPCDAHRGLQCDYSATYPGGPGQCVGQNQLGCELNGRRLEEGQVFQPSCAQLCHCLGGGVTCVPLCTDDLKPPPAERCPHPQLLRPPGRCCKEWVCDGLDNSISTGQPAAELAPQDQGPGSRPVPACVEWSTDWSPCSHSCGPGVSTRVSNRNRFCRPQTQTRLCQVRPCQSLMLPVSPHLVWPTGFSSLVPVQLEHQGCWSIRPYWPRFCAPSCPDGHCCSPALTRTVAMAFLCPQRRLVQYQVMVIESCSCSVSNCRPLSRSTVLPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACV6</accession>
    <name>A0A673ACV6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LHFPL tetraspan subfamily member 4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ACV6"/>
    <dbReference type="Ensembl" id="ENSSORT00005027881.1">
        <property type="protein sequence ID" value="ENSSORP00005027096.1"/>
        <property type="gene ID" value="ENSSORG00005012958.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ACV6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019372">
        <property type="entry name" value="LHFPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489:SF17">
        <property type="entry name" value="LHFPL TETRASPAN SUBFAMILY MEMBER 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489">
        <property type="entry name" value="LIPOMA HMGIC FUSION PARTNER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10242">
        <property type="entry name" value="L_HMGIC_fpl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="266" mass="28816" checksum="40464896C9A588BA" modified="2020-06-17" version="1">MSASPPLSDLSRLYQTEFVRSARAVGVLWAVCTLCFAIIQVVVLVQPSWIGTAGSHHQGVGPAPPSGTLGLFEVCVESDWPVPDCRGSLSSLSPLPSFQSVAVLVGVSLWAVWTSVLCLCLFRFCSAATVYKICAWLQLTAGRTHRTQPTRQTQLFEPRAFNDVGSFSPGNCSVHWAYILAILGVLDAAILATLAFVLANRQDALLPPDAKDGEEGAGHEPHRKPLQSDCLLMKVCFISYYRFTDVSVECFYLMLSLISCVASISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADK8</accession>
    <name>A0A673ADK8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">V-set and transmembrane domain containing 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ADK8"/>
    <dbReference type="Ensembl" id="ENSSORT00005028162.1">
        <property type="protein sequence ID" value="ENSSORP00005027376.1"/>
        <property type="gene ID" value="ENSSORG00005013062.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ADK8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF8">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="169"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="285" mass="32522" checksum="DE64A49C1ADF6554" modified="2020-06-17" version="1">MVTERDNLTLSCLVSQRKRSNSVLILRWFFTPPAAPTMGIKKMKLYGNYTRRFPQPKFRLYEENEGEIYRLRIFNVTGMDQGFYTCRVQEIRKHRNTWRASSNGTSTTHLTVHFTLEDGNSEGLWRLFADVYLCAVLICSLGLLSIFLFTVALTCQYFYRRHRLKGTTNSFSSSSYSSGETVTSSSSSSSSSPRAQRKVTKQKSDRKVTITPPKPPEEPPPHIPVKGKTDVTFWTPRVIQDDSLTYAELELVRPRPEPPASTNPDPASSNSDTVYAQILFQERQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADY7</accession>
    <name>A0A673ADY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ADY7"/>
    <dbReference type="Ensembl" id="ENSSORT00005028232.1">
        <property type="protein sequence ID" value="ENSSORP00005027444.1"/>
        <property type="gene ID" value="ENSSORG00005013102.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ADY7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF31">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="39"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="149" mass="16477" checksum="78F2973A00DBF373" modified="2020-06-17" version="1">GSDPQPDQVLDPAQSCECRCDPLNKTERTHVPEVDQPSCSLCQKVQSDPVRTSCGHWLCRQCITSYWDQSGSSGDSSCPQCEETSRTRAELQIHSSVQSKTGHLSEHVCTSLNTTCVQLSLTEHHNKEHLNLVSIVTGLGFVIWKLLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEF7</accession>
    <name>A0A673AEF7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Immunoglobulin lambda-1 light chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115424802</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AEF7"/>
    <dbReference type="Ensembl" id="ENSSORT00005028791.1">
        <property type="protein sequence ID" value="ENSSORP00005027990.1"/>
        <property type="gene ID" value="ENSSORG00005013403.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028805.1">
        <property type="protein sequence ID" value="ENSSORP00005028004.1"/>
        <property type="gene ID" value="ENSSORG00005013403.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028807.1">
        <property type="protein sequence ID" value="ENSSORP00005028006.1"/>
        <property type="gene ID" value="ENSSORG00005013403.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07699">
        <property type="entry name" value="IgC1_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000283">
        <property type="entry name" value="Immunoglobulin kappa constant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044627723" evidence="8">
        <location>
            <begin position="18"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="140"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="242" mass="26067" checksum="92C0E51646E73B77" modified="2020-06-17" version="1">MLVTLCTLITALTYVDAAKVLTQTPAVLTVSAGQEVVLKCNIQRDDGYYVSWYKQVPGGAPQFVMDFYHTDSDTSYGTGFSSDRFNSKTTSDIDYQLIISRAEVGDSAQYFCGTWDDSADEHVFGPGTKLTVTSSSVPPPVLTVFPPSAAELQSNKATLVCLSSQSVAFAHVTWLAAGSPLSTGISTSTAVQQPDQTFQISSYLSIQTSDWNTDQVYTCQVSVGSQTSEKNINKSACPTEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEJ9</accession>
    <name>A0A673AEJ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AEJ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005028500.1">
        <property type="protein sequence ID" value="ENSSORP00005027706.1"/>
        <property type="gene ID" value="ENSSORG00005013242.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AEJ9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="123"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="162" mass="18831" checksum="718D133EEAAD1E78" modified="2020-06-17" version="1">PLRCPSAWDRALAVVVQAYEGMESVLLPCQYSGKLPVVPSVVWRRSDLNQTVIYLLKDESDDVKEEYSDRMSMKTNVLESKDFSLTVKKPQLSDSSNYTCNLQWETEWKETKEWRLTQVQLQVKGQTQTTPTAVDTGQRSDKNHTHYSRHRSEVRYKPHPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGS8</accession>
    <name>A0A673AGS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Talin N-terminal F0 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AGS8"/>
    <dbReference type="Ensembl" id="ENSSORT00005029268.1">
        <property type="protein sequence ID" value="ENSSORP00005028459.1"/>
        <property type="gene ID" value="ENSSORG00005013617.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGS8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030160">
        <property type="term" value="F:synaptic receptor adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000029">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051569">
        <property type="entry name" value="SHANK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135:SF4">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16504" checksum="9D3C84A032659BCC" modified="2020-06-17" version="1">IRGASSDSGSSREEDSGIPIPVCVPAFRPGAERSHSARGPMEEPTGNTVVIRIGIPDLQQTKCLKFNVDAPIWLSKQRILCTLNQSLKDVLNYGLFQPAYNGKAGKFLDEERQLREYPFPSIAPVPYLEVGLKPCVFMRLLNVSAGQCEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AK78</accession>
    <name>A0A673AK78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 24">ATP-citrate synthase</fullName>
            <ecNumber evidence="6 24">2.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20 24">ATP-citrate (pro-S-)-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21 24">Citrate cleavage enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">aclyb</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>oxaloacetate + acetyl-CoA + ADP + phosphate = citrate + ATP + CoA</text>
            <dbReference type="Rhea" id="RHEA:21160"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:21162"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5 24">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 24">In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 24">In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.8" evidence="6 24"/>
    <dbReference type="AlphaFoldDB" id="A0A673AK78"/>
    <dbReference type="Ensembl" id="ENSSORT00005030949.1">
        <property type="protein sequence ID" value="ENSSORP00005030105.1"/>
        <property type="gene ID" value="ENSSORG00005014354.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AK78"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003878">
        <property type="term" value="F:ATP citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06100">
        <property type="entry name" value="CCL_ACL-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000004">
        <property type="entry name" value="ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000009">
        <property type="entry name" value="ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.110:FF:000001">
        <property type="entry name" value="ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000003">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000004">
        <property type="entry name" value="ATP-citrate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000024">
        <property type="entry name" value="Probable ATP-citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014608">
        <property type="entry name" value="ATP-citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032263">
        <property type="entry name" value="Citrate-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056749">
        <property type="entry name" value="Citrate_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23118:SF42">
        <property type="entry name" value="ATP-CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16114">
        <property type="entry name" value="Citrate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24948">
        <property type="entry name" value="Citrate_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036511">
        <property type="entry name" value="ATP_citrt_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 24" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 24" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="19 24" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 24" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CoA-binding" evidence="27">
        <location>
            <begin position="494"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="25">
        <location>
            <position position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRNR" id="PIRNR036511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR036511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1103" mass="121060" checksum="8C1033773A2A5E5E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANL1</accession>
    <name>A0A673ANL1_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ANL1"/>
    <dbReference type="Ensembl" id="ENSSORT00005031708.1">
        <property type="protein sequence ID" value="ENSSORP00005030844.1"/>
        <property type="gene ID" value="ENSSORG00005014724.1"/>
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        <location>
            <begin position="1"/>
            <end position="1233"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
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            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
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        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="640"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="731"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="965"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1121"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1199"/>
            <end position="1218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1273" mass="121301" checksum="F19CADF05BD5A4A1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANM7</accession>
    <name>A0A673ANM7_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">Aladin seven-bladed propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">aaas</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029994921.1">
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    </dbReference>
    <dbReference type="GO" id="GO:0005643">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    <dbReference type="SUPFAM" id="SSF50978">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="132"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="520" mass="56423" checksum="121B5908366F367E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQN4</accession>
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        <recommendedName>
            <fullName evidence="8">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pi15a</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
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        <property type="interactions" value="21"/>
    </dbReference>
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        <property type="gene ID" value="ENSSORG00005014691.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQN4"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Peptidase inhibitor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025673850" description="SCP domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="8">
        <location>
            <begin position="66"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="259" mass="29103" checksum="AF1121E4CCD22192" modified="2020-06-17" version="1">MKSHLFAVDLILLCISVGASALATTIPAVSASLPAANFTNLGAAHSYGPDTTSISKTRRKRYISQNDMLAILDYHNKVRGKVFPPASNMEYMVWDDTLAKTAEDWAHACLWEHGPSHLLRFLGQNLSVRTGRYRSILQLVKPWYDEVKDYSFPYPRDCNPRCPLRCYGPMCTHYTQMVWATSNKVGCAVHTCHNMNVWGSVWKRATYLVCNYSPKGNWIGEAPYKVGVPCSACPPSYGGSCSNNMCFPALKTNYLHWFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ARQ0</accession>
    <name>A0A673ARQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta-chimaerin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Beta-chimerin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Rho GTPase-activating protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">chn1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ARQ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005033093.1">
        <property type="protein sequence ID" value="ENSSORP00005032206.1"/>
        <property type="gene ID" value="ENSSORG00005015206.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ARQ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000005">
        <property type="entry name" value="Chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000025">
        <property type="entry name" value="Chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051854">
        <property type="entry name" value="Rho-type_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46075">
        <property type="entry name" value="CHIMERIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46075:SF6">
        <property type="entry name" value="N-CHIMAERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="10">
        <location>
            <begin position="80"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="159"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="350" mass="39907" checksum="8D02764A938414C1" modified="2020-06-17" version="1">MPSRESYEVCKEEKSLVQKAKREANQEDILAAALGMRMGPQKPPATFWQPLKLFAYSQLTSLVRRATLKESDRTSKSEKVHNFKVHTFRGPHWCEHCASFMWGLMAQGVKCAASYILKQKISLKIEQINCGLNVHKQCSNLVPNDCKPDLRHIRRVYSCDLTTLVKAYNTARPMVVDMCIREIESRGLKSEGLYRISGFSDSVEEVKTAFDKDGEKTDISVNAYEDINIITGALKMYLRELPVPVISYDAYPRFIEAAKLTDPEKKLEAFREALALLPQSHSETLKYLMAHLKRVTQNENFNLMNAENLAIVFGPTLMRAPNLDAITALNDIRYQRQVVEVLIKNQDVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASK6</accession>
    <name>A0A673ASK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">2-aminomuconic semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="9">1.2.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aldehyde dehydrogenase family 8 member A1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">aldh8a1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NAD-dependent oxidation of 2-aminomuconic semialdehyde of the kynurenine metabolic pathway in L-tryptophan degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-aminomuconate 6-semialdehyde + NAD(+) + H2O = (2Z,4E)-2-aminomuconate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14469"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:77634"/>
            <dbReference type="ChEBI" id="CHEBI:77859"/>
            <dbReference type="EC" id="1.2.1.32"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid degradation; L-kynurenine degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.32" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673ASK6"/>
    <dbReference type="FunCoup" id="A0A673ASK6">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033201.1">
        <property type="protein sequence ID" value="ENSSORP00005032314.1"/>
        <property type="gene ID" value="ENSSORG00005015331.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ASK6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047102">
        <property type="term" value="F:aminomuconate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001758">
        <property type="term" value="F:retinal dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042573">
        <property type="term" value="P:retinoic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07093">
        <property type="entry name" value="ALDH_F8_HMSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000021">
        <property type="entry name" value="Aldehyde dehydrogenase family 8 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720:SF2">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="14">
        <location>
            <begin position="27"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="490" mass="53283" checksum="47CFD0EDCB49E018" modified="2020-06-17" version="1">KSKDIQTLSFTQRFNYIGGQFVPCRSYIDSYNPSTGEVYCKVPDSGEEEVEAAVAAAKEAFPEWSARSAEQRAKVLNKLADLIDANLEELAQAESRDQGKTVTFARTVDIPRSAYNFRFFASSVLHHTTDCSQMDHMGCLNYTIRCPVGVAGLISPWNLPLYLLTWKIAPAIATGNTVVAKPSEMTSVTAWMICKLMGQAGVPPGVVNMVFGTGPKAGHALVGHPEVPLISFTGSTATAQRITERSAPYCKKLSLELGGKNPAIIFADADLDQCIETTVRSSFSNQGEICLCTSRIYVERSIYSTFLERFVAAARKWKTGVPSDPSSNNGALISKEHLQKVQSFVELAKSEGGIVHCGEGVDQLHLPAANANGYFMPATVISGISDSSRVMQEEIFGPVTCISPFDTEDEAVSKGNGVRYGLAATVWSRDVGKVHRVAKRLQAGLVWTNCWLVRDLNLPFGGMKNSGVGREGGKDSYHFFTEVKSITIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUQ3</accession>
    <name>A0A673AUQ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AUQ3"/>
    <dbReference type="Ensembl" id="ENSSORT00005033968.1">
        <property type="protein sequence ID" value="ENSSORP00005033064.1"/>
        <property type="gene ID" value="ENSSORG00005015686.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUQ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF14">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="14"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="212" mass="24147" checksum="1926678124A910ED" modified="2020-06-17" version="1">MFISSFKTEKVYHYVPNLPEPTSRAELTQYWMNLSLDDKTANKMLWISDNSSTVSRRTKEVCPVLDRPERYEYSPQVLCREGIWNMRAYWEVEFSGWVVIGATYEGAGRRANSGPSGLGENDESWGLCWSGSMYQIWFNGVNKDISDVTFSPTIGVYIDQPAGIINFYTVTGVGAEKEVKLLHRVQTTIEKKLLPGFWVGMQSSCKLLQKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AVA2</accession>
    <name>A0A673AVA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein Hox-D4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hoxd4a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Antp homeobox family. Deformed subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AVA2"/>
    <dbReference type="FunCoup" id="A0A673AVA2">
        <property type="interactions" value="456"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033169.1">
        <property type="protein sequence ID" value="ENSSORP00005032282.1"/>
        <property type="gene ID" value="ENSSORG00005015321.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AVA2"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000029">
        <property type="entry name" value="Homeobox protein Hox-D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050609">
        <property type="entry name" value="Antp_homeobox_Deformed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771">
        <property type="entry name" value="HOMEOTIC PROTEIN DEFORMED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="164"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="166"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="87"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="225"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="255" mass="29574" checksum="28F069DAB4F07F04" modified="2020-06-17" version="1">MEGGKKDNSRKISIFYLQKLMAMSSYMVNSKYVDPKFPPCEEYSQNNYIPEQGSDYYSPSQDTEFQHPGIYPRSNYTEQPFSCNTVQDSTVQPRGHVHERSTHSSPFTAQTERGPPVQVAGPRTCGQQQNTKSQNGIQAKQPAIVYPWMKKVHVTTVNPDYTGSEPKRSRTAYTRQQVLELEKEFHFNRYLTRRRRIEIAHTLCLSERQIKIWFQNRRMKWKKDHKLPNTKGRSAPASSHLQSAQKDNQTDITAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AX89</accession>
    <name>A0A673AX89_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AX89"/>
    <dbReference type="Ensembl" id="ENSSORT00005035192.1">
        <property type="protein sequence ID" value="ENSSORP00005034275.1"/>
        <property type="gene ID" value="ENSSORG00005016204.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AX89"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="124" mass="14235" checksum="1A4869341C9E0D88" modified="2020-06-17" version="1">MELIFNYSHCTDSILFYSILCSPGSSSILATMYMRSDPSMGLISKIQQHLPLSVHVSLSPQGQSLLCALLFSTLLWLSLILTLRFSLKLLLSYHRWMFETHTHMSHTTRVWGVRGSDWTLWWAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3G9</accession>
    <name>A0A673B3G9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        <text evidence="2">Belongs to the pleurocidin family.</text>
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    <keyword evidence="5" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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            <end position="76"/>
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            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00007419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="76" mass="8457" checksum="00B75ABEB8ADDD7C" modified="2020-06-17" version="1">MKGVMIFLVLSLVILMAEPGECFWGKLWRSGKAAVQAWNVLQFSQKGVPKPSSVSAGNVAPQQNPCRQIKHFMQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B543</accession>
    <name>A0A673B543_9TELE</name>
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        <submittedName>
            <fullName evidence="16">Glutamate receptor, metabotropic 8b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">grm8b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
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        <property type="component" value="Chromosome 6"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="mGluR"/>
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        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01054">
        <property type="entry name" value="MTABOTROPC4R"/>
    </dbReference>
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        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
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        <location>
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            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025681805" evidence="14">
        <location>
            <begin position="18"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="578"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="742"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="775"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="817"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="15">
        <location>
            <begin position="578"/>
            <end position="852"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="889" mass="98656" checksum="09614EC6B81B301F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5P9</accession>
    <name>A0A673B5P9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Transient receptor potential cation channel subfamily V member 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115432040</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030008671.1">
        <property type="nucleotide sequence ID" value="XM_030152811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B5P9"/>
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        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038602.1">
        <property type="protein sequence ID" value="ENSSORP00005037621.1"/>
        <property type="gene ID" value="ENSSORG00005017361.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="A0A673B5P9"/>
    <dbReference type="OrthoDB" id="533508at2759"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRPV"/>
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        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY V MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01768">
        <property type="entry name" value="TRPVRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 15" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="423"/>
            <end position="441"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="453"/>
            <end position="478"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="603"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="456"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037621.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="759" mass="86996" checksum="0E5646FE62977015" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B6N4</accession>
    <name>A0A673B6N4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="27">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">abl2</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22 27">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="23">Interacts with PSMA7. Interacts with CTTN. Found in a complex with ABL1, ABL2, CRK and UNC119; leading to the inhibition of CRK phosphorylation by ABL kinases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="27">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005036984.1"/>
        <property type="gene ID" value="ENSSORG00005017371.1"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:2000145">
        <property type="term" value="P:regulation of cell motility"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="PTKc_Abl"/>
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        <property type="entry name" value="SH3_Abl"/>
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        <property type="entry name" value="Tyrosine-protein kinase"/>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="SH3 Domains"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="ABL_SH2"/>
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    <dbReference type="InterPro" id="IPR015015">
        <property type="entry name" value="F-actin-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
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    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08919">
        <property type="entry name" value="F_actin_bind"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
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    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00808">
        <property type="entry name" value="FABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 26 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="10 26 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17 24" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4 25" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7 27" id="KW-0808">Transferase</keyword>
    <keyword evidence="18 27" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SH3" evidence="30">
        <location>
            <begin position="104"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="29">
        <location>
            <begin position="170"/>
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        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="285"/>
            <end position="536"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="552"/>
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    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="591"/>
            <end position="643"/>
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    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="655"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="774"/>
            <end position="945"/>
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    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="970"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="591"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="605"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="655"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="682"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="717"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="741"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="774"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
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            <begin position="832"/>
            <end position="847"/>
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            <begin position="850"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="892"/>
            <end position="903"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="28">
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            <begin position="912"/>
            <end position="933"/>
        </location>
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        <location>
            <begin position="970"/>
            <end position="979"/>
        </location>
    </feature>
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        <location>
            <begin position="1055"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1195" mass="129166" checksum="73F1EF5BA6B3E002" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B806</accession>
    <name>A0A673B806_9TELE</name>
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        <submittedName>
            <fullName evidence="15">ATP-binding cassette, sub-family A (ABC1), member 12</fullName>
        </submittedName>
    </protein>
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        <name evidence="15" type="primary">abca12</name>
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    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:positive regulation of cholesterol transport"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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        </location>
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            <end position="2817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="2824"/>
            <end position="2848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="2902"/>
            <end position="2923"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="3363"/>
            <end position="3384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="3396"/>
            <end position="3417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="3633"/>
            <end position="3657"/>
        </location>
    </feature>
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            <begin position="3689"/>
            <end position="3710"/>
        </location>
    </feature>
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        <location>
            <begin position="3717"/>
            <end position="3737"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="3749"/>
            <end position="3772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="3784"/>
            <end position="3803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="3837"/>
            <end position="3858"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="3001"/>
            <end position="3232"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="3905"/>
            <end position="4143"/>
        </location>
    </feature>
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            <end position="4241"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="4241" mass="475330" checksum="52A062CC589A2E7A" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B883</accession>
    <name>A0A673B883_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium/glucose cotransporter 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Low affinity sodium-glucose cotransporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Solute carrier family 5 member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">slc5a2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1:1. Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump. Unlike SLC5A1/SGLT1, requires the auxiliary protein PDZK1IP1/MAP17 for full transporter activity. Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-glucose(out) + Na(+)(out) = D-glucose(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70571"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70572"/>
        </physiologicalReaction>
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    <comment type="subunit">
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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</entry>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24141">
        <property type="entry name" value="2-5A-DEPENDENT RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24141:SF1">
        <property type="entry name" value="2-5A-DEPENDENT RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="183"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="281"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="323"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="486"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="552" mass="61637" checksum="74FD193A45D403CB" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BAS7</accession>
    <name>A0A673BAS7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BAS7"/>
    <dbReference type="Ensembl" id="ENSSORT00005038540.1">
        <property type="protein sequence ID" value="ENSSORP00005037563.1"/>
        <property type="gene ID" value="ENSSORG00005017629.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BAS7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032801">
        <property type="entry name" value="PXL2A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13911">
        <property type="entry name" value="AhpC-TSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="127" mass="14471" checksum="AFF66640ECCA10F6" modified="2020-06-17" version="1">HNQKSRSLDAHSVEVVIISFGCREGASHWLEETGFQYDMLLDSDRELYTAFGLGASVRKVLNFSNMLLYAEYVMDHMEFPRGLPSIQDDMFQLGGDFVLDEYGTVLFSHRCHSPIDRPTVDHILSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB22</accession>
    <name>A0A673BB22_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Coiled-coil domain-containing protein 104</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cfap36</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CFAP36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BB22"/>
    <dbReference type="FunCoup" id="A0A673BB22">
        <property type="interactions" value="517"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038640.1">
        <property type="protein sequence ID" value="ENSSORP00005037658.1"/>
        <property type="gene ID" value="ENSSORG00005017668.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BB22"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1520.10">
        <property type="entry name" value="ADP-ribosylation factor-like 2-binding protein, domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023379">
        <property type="entry name" value="BART_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042541">
        <property type="entry name" value="BART_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038888">
        <property type="entry name" value="CFAP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21532">
        <property type="entry name" value="PHOSPHODIESTERASE HL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11527">
        <property type="entry name" value="ARL2_Bind_BART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BART" evidence="11">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="321"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="384" mass="43178" checksum="13CF27D60F734C5E" modified="2020-06-17" version="1">MAEDDREWVVESIVGYLGSPEWVIPVTDFMENKCTVFDDEDENKLSYTEIHQQYKKLVEKLLENYMQEVGINEQQFLDACTSPFAKSKTLQIVFQPVLATDDFQMFRSLMVQKNMELQLQALRVIKERNGALPDCLTDGTDVMTELQQQEMRILQEVLKKSKEEYDEEMSMRMLLEEEVGSTSRSSSDKPVRESGEAQQETSASSQQNSTAKVNSKPVSKEETKATATGGRDGKNSAKGSSSSKLTTDCGSNGLEPRVLPAVRAPVKSSELPSTKEQSSSSQTAAEAWVEEARKEAGISKPYTELSLSQQEQLQQRAAYLRQQRDKLHALKKEQQKSKQSSTAEETPSNPTPAPATPEISVEEKKKLQKRKHLADKLKEEVIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEH4</accession>
    <name>A0A673BEH4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">3 beta-hydroxysteroid dehydrogenase/Delta 5--&gt;4-isomerase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hsd3b1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BEH4"/>
    <dbReference type="FunCoup" id="A0A673BEH4">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041202.1">
        <property type="protein sequence ID" value="ENSSORP00005040165.1"/>
        <property type="gene ID" value="ENSSORG00005018731.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BEH4"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000220">
        <property type="entry name" value="3 beta-hydroxysteroid dehydrogenase/Delta 5--&gt;4-isomerase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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</entry>
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    </dbReference>
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        </source>
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    <sequence length="1306" mass="142634" checksum="F74AE64994ED81CB" modified="2020-06-17" version="1"> </sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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    </evidence>
    <sequence length="737" mass="68747" checksum="21C2E2D632AEB669" modified="2020-06-17" version="1"> </sequence>
</entry>
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        <submittedName>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="376" mass="42735" checksum="6F846F9F16A6FF79" modified="2020-06-17" version="1">MSMRTLPLVFINLGGEMLYILDQRLQAQNTSEDNSEKGMWSENDRKRVMNDIVGTMFSKSFMDELLKPQQLYSHRTMKTVLTRLAHTSIMRLNPASMDRLYELMVMAFKYQVFLCPRPKDLLLISYNHIDTIREFVKDTPAVLNQVEETHRKIIEVYSSLSEGEFQLLRQTLLIFFQDLHVRVSLFLKNKVQNPNGRFALGTSGPVPYKTDVPGLIRAFDRKGREVRRHEFPTGGSYSSPLRVGSFDLHGDRVIRLGLNMYVSSVNCYNTPNLLAKEELNLLARLMGSMKAEHTPNTETVFRINLFTTDAEEEEAGASGGTEESLFGVINIQAMQDEQAATELARIAGQFMEQDEPVENPSSSKGDDLLAMMDDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLT5</accession>
    <name>A0A673BLT5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Anti-dorsalizing morphogenic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">admp</name>
    </gene>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BLT5"/>
    <dbReference type="Ensembl" id="ENSSORT00005042280.1">
        <property type="protein sequence ID" value="ENSSORP00005041218.1"/>
        <property type="gene ID" value="ENSSORG00005019201.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLT5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035239">
        <property type="term" value="P:tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13765">
        <property type="entry name" value="TGF_beta_ADMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000023">
        <property type="entry name" value="Anti-dorsalizing morphogenetic protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF241">
        <property type="entry name" value="ANTI-DORSALIZING MORPHOTIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025563841" evidence="10">
        <location>
            <begin position="22"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="11">
        <location>
            <begin position="272"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="242"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="391" mass="43809" checksum="D5321C9C7130AB96" modified="2020-06-17" version="1">MCSAMLLFGIGLVTLLCATDARPSLNYLEDHFTVENESEEVRSAAIKRLLEVFGMDDTPAIHGHKQPPQYMLDLYNTVADVDGVTKDPYLLEGNTVRSFFDKLHSEQVEFRFNLSTVSRAEKILTAELHLFKLRPQATLTFNRHHFCQVSVYQLLNSKSNSTQDKKLLSSRLIPVHSTGWEVFTITQAVRSWMLDEGSNLGLHVVVQTLGGSPMDMKLIRFASGRNHHQSKQPMLVLFTDDGRRSSKPLESTDPNDATSNLPRPLLSSPSSRSVRSVDESEEEGASMSCQRLPLYVDFEEIGWSGWIVSPRGYNAYHCKGSCPFPLGQNMMPTNHATVQSIINALKLIKGIDMPCCVPDKLFSINLLYFDDDENVVLKQYNDMVAGSCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BN37</accession>
    <name>A0A673BN37_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gjb9b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BN37"/>
    <dbReference type="FunCoup" id="A0A673BN37">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043094.1">
        <property type="protein sequence ID" value="ENSSORP00005042022.1"/>
        <property type="gene ID" value="ENSSORG00005019482.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BN37"/>
    <dbReference type="OrthoDB" id="9441654at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF118">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="13">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="14">
        <location>
            <begin position="139"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="238"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="273" mass="31243" checksum="0A00C7E1CA1CF1A1" modified="2020-06-17" version="1">MNWSALEALISGVNKYSTVFGRVWLSMVFVFRVMVFVVAAQRVWGDENKDFVCNTAQPGCNNVCYDHIFPISHIRLWALQLIFVTCPSLLVVGHVKYREKKDSQYTVSHKGAHLYANPGKKRGGLWWTYLLSLVFKAGFDAGFLYILYHIYDGYDMPRLSKCSLAPCPNTVDCFISRPTEKKIFTLFMVVSSVFCILMCICEMVYLICKRILKLLKRKNEAERRMFAETHEMTPLAAPRSEFRSKTSVRVDPTAASTQNLSNIKAEEGTSRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BPD8</accession>
    <name>A0A673BPD8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Proteinase-activated receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Thrombin receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115430572</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030006522.1">
        <property type="nucleotide sequence ID" value="XM_030150662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BPD8"/>
    <dbReference type="Ensembl" id="ENSSORT00005043540.1">
        <property type="protein sequence ID" value="ENSSORP00005042462.1"/>
        <property type="gene ID" value="ENSSORG00005019680.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430572"/>
    <dbReference type="InParanoid" id="A0A673BPD8"/>
    <dbReference type="OrthoDB" id="8881832at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015057">
        <property type="term" value="F:thrombin-activated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030194">
        <property type="term" value="P:positive regulation of blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15369">
        <property type="entry name" value="7tmA_PAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000040">
        <property type="entry name" value="Coagulation factor 2 (thrombin) receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003912">
        <property type="entry name" value="Protea_act_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000935">
        <property type="entry name" value="Thrmbn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF20">
        <property type="entry name" value="PROTEINASE-ACTIVATED RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01428">
        <property type="entry name" value="PROTEASEAR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00908">
        <property type="entry name" value="THROMBINR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025682467" description="Proteinase-activated receptor 1" evidence="18">
        <location>
            <begin position="20"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="105"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="161"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="440" mass="49221" checksum="9F1FB7AEA88A2344" modified="2020-06-17" version="1">MDSKSFLLLLVFACTATAAFKNNSVGARSFAYTNHLVTDEPLDLDYLLGEGAAKMNHSEGGGMRNKTYTLMQNISEEALLFLTGPVSTILIPSCYTLVCLISVPINICAVMAFAQRIRPKKPAAIYMLNLACADLIFALLLPFKISYHFAGNNWIFGPIMCRVVTAAFYWNMYSSVLLVTCISVDRLLAVVYPIDSLVWRRPRNAIIACVTMWILSFAGSVPLVLSNQTVYLNQLDITTCHDVQLLDDLKWHYKIYFITICSVLFFLPLLITVVSYTRVIWALSRVPHGVPGRSRRKTRAVVMALTVLVMFVLCFMPTNTALLVHYLQFNEGVQNSEEAPNGSYALYLVFLCLGSLNCCLDPMVYYFGSSQCQRQLSSVLRCEKSLVSSISHSSSDSYRSNSRTVLKSSCRTESTKINPPVSENDSFQTNLNSQYKKLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQ46</accession>
    <name>A0A673BQ46_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BQ46"/>
    <dbReference type="Ensembl" id="ENSSORT00005043406.1">
        <property type="protein sequence ID" value="ENSSORP00005042328.1"/>
        <property type="gene ID" value="ENSSORG00005019619.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQ46"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="200" mass="22250" checksum="E22C85DF91192450" modified="2020-06-17" version="1">RYLRHLNASHVQLCLPKHTRTPECITSKLCLLKHTQTPECITSKLCLPKHTRTPECITSKLCLLKHTQTPECITSKLCLPKHTRTPECITSKLCLLKHTQTPECITSKLCLLKHTQTPECITCPTSAYRNTHRPPECITSKLCLLKHTRTAETETEQSHTDEPGPGPSRSVSECGPGPGSGCVTFDGLLQILQVELVLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRV0</accession>
    <name>A0A673BRV0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673BRV0"/>
    <dbReference type="Ensembl" id="ENSSORT00005045659.1">
        <property type="protein sequence ID" value="ENSSORP00005044535.1"/>
        <property type="gene ID" value="ENSSORG00005020507.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRV0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000021">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF48">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY, POLYPEPTIDE C2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="350" mass="39732" checksum="29E5BF29170EF636" modified="2020-06-17" version="1">MSRTTLPTTMQSQFLSKKPLIMTYESHPRKNHCHRKGRDVISYICSFAGEMFTAMYNMHRIICDMATGMFKDKTLMRSLNETEYDMVLTDPAWGAGILLSHYLKIPLVYNVRWITSGEGHLAAAPSPLSYIPMTVSGLSDKMTFIQRVKNVLFYVMWQAQDGFLINPQYQAVCDKFFGSEVRYSDLLQGADLWLMRVDFVFEFPRPTMPNFVYIGGFQCTPAKPLPQHLEDFVQSSGEHGVILMSLGTFVSALPADMTNEIAAAFAKLPQKVIWRHKVDWMPQNDLLGHPKMKLFVAHGGTNGLQEAIYHGVPVVGLPVFFDQYDNLVPVKYTENNYLSYNLLVMGLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTX3</accession>
    <name>A0A673BTX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the Deltex family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673BTX3"/>
    <dbReference type="Ensembl" id="ENSSORT00005046371.1">
        <property type="protein sequence ID" value="ENSSORP00005045235.1"/>
        <property type="gene ID" value="ENSSORG00005020774.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BTX3"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09633">
        <property type="entry name" value="Deltex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.130:FF:000001">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase DTX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039396">
        <property type="entry name" value="Deltex_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039399">
        <property type="entry name" value="Deltex_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039398">
        <property type="entry name" value="Deltex_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048409">
        <property type="entry name" value="DTX3L_KH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12622">
        <property type="entry name" value="DELTEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18102">
        <property type="entry name" value="DTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21718">
        <property type="entry name" value="KH_DTX3L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="140"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="92"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="319" mass="35238" checksum="FFF0FCBA3BCC9290" modified="2020-06-17" version="1">MSIKDPAIADGLTIDEDDWKLITTSYSKDLEKIKAKFGVDFKESGITQGKTTVKMFYKRSGGNPSMESHAVRALLRLYQKVAMSPMNAHWVLGDSSKNRSSVDEESEGASAGPFMNGQSRNTEASTVGGATGGKDEEDKCPICLDTFNNKTQLKCKHEFCEECLENSKKSTGAMCPVCKDVFGLIEGDQPDGQMTWDKSHISLPGFPKCGSIRIHYHIPSGIQTERHPKPGNSYTGISRTAYLPDNKEGNEVLLLLKKAFDQRLIFTVGMSRTTGLDNQVTWNDIHHKTSMIGGPQSFGYPDPDYLTRVKEELQAKGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BU55</accession>
    <name>A0A673BU55_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 184C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115438892</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BU55"/>
    <dbReference type="Ensembl" id="ENSSORT00005045242.1">
        <property type="protein sequence ID" value="ENSSORP00005044122.1"/>
        <property type="gene ID" value="ENSSORG00005020339.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BU55"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="383"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="444" mass="50600" checksum="A560FB0DFD2FB155" modified="2020-06-17" version="1">MPCSCAEWRRWIRPLVLVLYALLLVAVLPLCIWELQKDQVPHCLYIQIHFCCIQVGTHSKAWFIAGVFVFLTIPVSLWGILQHMVHYTQPELQKPIIRILWMVPIYSLDSWLALRYPSLAIYVDTCRECYEAYVIYNFLVFLLNFLSNQYPSLVLMLEVQQQQNHLPPLCCCPPWPMGECQLLNVICQLCGVYDEANFSFRNAWSYLVIINNISQLFAMYCLVLLYCALREELTPIRPVGKFLCVKLVVFVSFWQAVLIAFLVKVGVISDKHTWDWDSVEAVATGLQDFIICIEMFLAAIAHHYTFTYKPYVQEAEEGSCFDSFLAMWDFSDIQADVTEQVRHVGRTFLGRPNKMYFGTLARPEHTEHTGLLTASSQDPLVAAATSMPSSPSSSGRYQGLGHTPAPHSISAPAGFTSSSWEDDNGWIYSELKDTSHSVMSKPCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVC2</accession>
    <name>A0A673BVC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115433940</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BVC2"/>
    <dbReference type="Ensembl" id="ENSSORT00005046930.1">
        <property type="protein sequence ID" value="ENSSORP00005045775.1"/>
        <property type="gene ID" value="ENSSORG00005021011.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVC2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390:SF235">
        <property type="entry name" value="GH09339P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="318" mass="35661" checksum="E2C89F8118AFF5DC" modified="2020-06-17" version="1">MPVQSCVQTPGEIHLRDAAAEALPPSTDSSPFLHTQSDLPDHQYARPSSQMLPEGVGVSTRRSRKRQGSRWMKTSDKPTEGLDWSHACSLCRMLFPNAERLSDHHRKSHPVCSVCGTLFTGVLKLRQHEISEHGLLPFTCNYCPKRFNHKAHRDLHVKTRHTGEKTCQCDICGKGYACISVMKTHRLTHFDKAFICSICGKSFYHACHLTRHKMVHQDERPHRCPTCGRGFTQAANLRSHQVIHSGERELCSICGKRYRCLRNHLISKHSDELPLTQTVISCNVCGKKFPTPHSSKFTRGATPARGPLAATSVGRATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BW67</accession>
    <name>A0A673BW67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115424640</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPI-coated vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Endoplasmic reticulum membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029997873.1">
        <property type="nucleotide sequence ID" value="XM_030142013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BW67"/>
    <dbReference type="FunCoup" id="A0A673BW67">
        <property type="interactions" value="1607"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045948.1">
        <property type="protein sequence ID" value="ENSSORP00005044817.1"/>
        <property type="gene ID" value="ENSSORG00005020606.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424640"/>
    <dbReference type="InParanoid" id="A0A673BW67"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="212" mass="24357" checksum="9C73B726B1C27492" modified="2020-06-17" version="1">MNIFRLTGDLSHLAAIIILLLKIWKSRSCAGISGKSQILFALVFTTRYLDLLTSFISLYNTCMKVIYIGCSYATVYLIYMKFRATYDGNHDSFRVEFLVVPVGGLAVLINHDFAVLEILWTFSIYLESVAILPQLFMISKTGEAETITTHYLFCLGMYRALYLFNWIWRFYFEGFFDMIAIVAGVVQTVLYCDFFYLYVTKVLKGKTLSLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZK5</accession>
    <name>A0A673BZK5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prolargin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">prelp</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BZK5"/>
    <dbReference type="FunCoup" id="A0A673BZK5">
        <property type="interactions" value="551"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047269.1">
        <property type="protein sequence ID" value="ENSSORP00005046112.1"/>
        <property type="gene ID" value="ENSSORG00005021126.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BZK5"/>
    <dbReference type="OrthoDB" id="5789657at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000133">
        <property type="entry name" value="prolargin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF8">
        <property type="entry name" value="PROLARGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025342778" evidence="6">
        <location>
            <begin position="23"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="7">
        <location>
            <begin position="72"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="379" mass="42812" checksum="B42192A450743B97" modified="2020-06-17" version="1">MKAGVGLFSALALFLLMGAVFTQRPRPKKPTRRPATTRRPSVPQPPAPGQPEPQEPTDYPPVILGMSSFFPDCPKECTCTPNYPNALNCENHNLRVIPVIPSRTHYLYLQNNFISEVTAEPFLNATDVRWINLANNRIHRIDKQVFGKIPSLLYLYIQRNQLKEVPSGLPASLEQLRLARNRISKIPSGAFSKMGNLTLLDLYHNQLSDNDLGKNTFKDLKSLMQLNLARNVLKKMPDGVPNGLIQLFLDRNLIDDIPRDYFKGFTHLAFVRLNYNQLSDKGLPKAVFNISTLLDLQLSHNQLASVPLFSSHLEHLHLDHNSIEVINGTQICPCSLDTDTSDQSLVPKLRYLRLDGNHLNPPIPVDVIMCFRHLHSIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0L6</accession>
    <name>A0A673C0L6_9TELE</name>
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        <submittedName>
            <fullName evidence="4">PDZ domain containing ring finger 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pdzrn4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005045933.1"/>
        <property type="gene ID" value="ENSSORG00005021060.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd06716">
        <property type="entry name" value="PDZ2-PDZRN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ domain containing ring finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3_ubiquitin-PDZ_ligase"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545">
        <property type="entry name" value="PDZ DOMAIN CONTAINING RING FINGER PROTEIN 3, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545:SF6">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING RING FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ domain-like"/>
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    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="129"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="552"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="533"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="731" mass="82836" checksum="FC25B59153B2FCC5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C132</accession>
    <name>A0A673C132_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">scn8ab</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="22">Pore-forming subunit of a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues.</text>
    </comment>
    <comment type="function">
        <text evidence="23">Pore-forming subunit of a voltage-gated sodium channel complex assuming opened or closed conformations in response to the voltage difference across membranes and through which sodium ions selectively pass along their electrochemical gradient. Contributes to neuronal excitability by regulating action potential threshold and propagation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Voltage-gated sodium (Nav) channels consist of an ion-conducting alpha subunit which is functional on its own associated with regulatory beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 26">Cell membrane</location>
            <topology evidence="2 26">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005047794.1">
        <property type="protein sequence ID" value="ENSSORP00005046632.1"/>
        <property type="gene ID" value="ENSSORG00005021347.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C132"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000006">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000005">
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        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000003">
        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008054">
        <property type="entry name" value="Na_channel_a8su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF23">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 8 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01667">
        <property type="entry name" value="NACHANNEL8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19 26" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13 26" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 26" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 26" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="17 26" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 26" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 26" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="701"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="738"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="819"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="909"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1153"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1192"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1223"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1269"/>
            <end position="1295"/>
        </location>
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        <location>
            <begin position="1394"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1430"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1486"/>
            <end position="1504"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1516"/>
            <end position="1538"/>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1550"/>
            <end position="1569"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1617"/>
            <end position="1638"/>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1659"/>
            <end position="1678"/>
        </location>
    </feature>
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        <location>
            <begin position="1706"/>
            <end position="1729"/>
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        <location>
            <begin position="1746"/>
            <end position="1781"/>
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            <begin position="1"/>
            <end position="48"/>
        </location>
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        <location>
            <begin position="417"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="536"/>
            <end position="564"/>
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        <location>
            <begin position="577"/>
            <end position="597"/>
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            <end position="1089"/>
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            <end position="1949"/>
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        <location>
            <begin position="8"/>
            <end position="46"/>
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    </feature>
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            <begin position="445"/>
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            <begin position="459"/>
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            <end position="1915"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
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        <source>
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        <source>
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        </source>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
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    <evidence type="ECO:0000256" key="18">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00038083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00055248"/>
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    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00058180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00061447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00064899"/>
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    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046632.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    <sequence length="1949" mass="221758" checksum="ED7A1AE7DECC8B52" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2C6</accession>
    <name>A0A673C2C6_9TELE</name>
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        <recommendedName>
            <fullName evidence="12">Ubiquitin-ribosomal protein eL40 fusion protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="primary">LOC115437146</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Component of the 60S subunit of the ribosome.</text>
    </comment>
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    </comment>
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        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
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        <text evidence="5">In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.</text>
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        <text evidence="4">In the N-terminal section; belongs to the ubiquitin family.</text>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002241"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010570"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="128" mass="14705" checksum="2BE411112F51CA98" modified="2020-06-17" version="1">MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLRQLAQKYNCEKMVCRKCYARLHSRAVNCRKKKCGHTSNLRPKKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2Q2</accession>
    <name>A0A673C2Q2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lysine acetyltransferase 14</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673C2Q2"/>
    <dbReference type="Ensembl" id="ENSSORT00005048714.1">
        <property type="protein sequence ID" value="ENSSORP00005047534.1"/>
        <property type="gene ID" value="ENSSORG00005021737.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C2Q2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF26">
        <property type="entry name" value="CYSTEINE-RICH PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="305"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="458" mass="51383" checksum="673905F9222CDC19" modified="2025-10-08" version="2">MRPEADKSTTKAKASKPTVDPIITVEGLRNAAPGTRWKTPSVTLTTSPPSAPQIERHSLAPHHRPSPDFSAPGTPASHSATPSLLSEADLIPDAMPPQALFTMDTEGMIDPGMEYIPPPNANLIARKKLRPVPPHIKREAESEDEEGCEREDDFEKPVGQGEGPPLLAGAKRLHRKLLVRQAKRQRGLPLLDIDRAVSAALSLVGGIYCAQEVGLLMGCGITGKYRTNSQELRVLDRFQVKVQTVLGHLVVKIPTSGTFLFHLMVTFWGSGLMFWTLVLDRSGTLSLYRFRRDYESRPVKLRLLAEIRSYCPQEGPKLVRPNHIPSVNAMCHDSFWPGVGPVRCLQYPDFSVVVLYKKVVIGFGFMVPDVKYNEAYISFLLVHPEWRRAGIGTFMIYHLIQTCMGKDVTLHVSASNPAMLLYQKFGFKAEEYILDFYDKYYPVDSTECRHAFFLRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C4S6</accession>
    <name>A0A673C4S6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">bnip3la</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C4S6"/>
    <dbReference type="FunCoup" id="A0A673C4S6">
        <property type="interactions" value="704"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049107.1">
        <property type="protein sequence ID" value="ENSSORP00005047922.1"/>
        <property type="gene ID" value="ENSSORG00005021879.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C4S6"/>
    <dbReference type="OrthoDB" id="5857140at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097345">
        <property type="term" value="P:mitochondrial outer membrane permeabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010548">
        <property type="entry name" value="BNIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186:SF3">
        <property type="entry name" value="BCL2_ADENOVIRUS E1B 19 KDA PROTEIN-INTERACTING PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186">
        <property type="entry name" value="RE48077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06553">
        <property type="entry name" value="BNIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="235" mass="25482" checksum="B38E0BB2F2779F02" modified="2020-06-17" version="1">MSTAAAQQNNNEEPGLHGSWVELELNGNTGAQGVQSNPLTTPAADQINANAGSQTPQTLQVVPESDETLIGGLEHVPSSSSIHNGDMEKILLDAQHESSPSNSSCDSPHRPPSPDQDEGQIMFDVEMSSLRGSQSEEDGLDKDREEDILMNKETDWVADWSSRPENIPPKEFHFRHPRRSVSLSMRKSGVMKKGGIFSAEFLKVVIPSLLISHILALGLGVYIGKRIATAPTSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAP2</accession>
    <name>A0A673CAP2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">B2 bradykinin receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115414108</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029983174.1">
        <property type="nucleotide sequence ID" value="XM_030127314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CAP2"/>
    <dbReference type="Ensembl" id="ENSSORT00005051030.1">
        <property type="protein sequence ID" value="ENSSORP00005049827.1"/>
        <property type="gene ID" value="ENSSORG00005022610.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414108"/>
    <dbReference type="InParanoid" id="A0A673CAP2"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004947">
        <property type="term" value="F:bradykinin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000496">
        <property type="entry name" value="Brdyknn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF957">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00425">
        <property type="entry name" value="BRADYKININR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="58"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="356" mass="40516" checksum="78AB3455786E7A3B" modified="2020-06-17" version="1">MVMTMDLPTSFSFPFSNTTIYEDLLENSTPCPETETQQMFSKVVPVYIFTLSVLGIVLNILVLLVFWLHKNPCTVPEIYLSNLAAADLLLMCFMPFWAVTIVNKYNWVFGPHMCRLVNLCIVMNANCSIYFLVLVSIDRFLALVHPLSHARMRSRKCAKFGCLLVWCFGLILSIPTLVCRKVKHKKSNITQCILGCSTTMFYTNEGILTVVCFIIPVMIISYCTVRIIRSLNKRPIESLGTQRKEYKSTILVLVVLVAFLICWVPFHLTRIIDVFLRAEIIPGHDSCDIVNTFEMFMQIFSYIAFLTASSTPILYVIVGKTFRDKTLEVFCEFTMKRVSTSGTTASRTLQSTGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFQ6</accession>
    <name>A0A673CFQ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pabpn1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029979282.1">
        <property type="nucleotide sequence ID" value="XM_030123422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CFQ6"/>
    <dbReference type="SMR" id="A0A673CFQ6"/>
    <dbReference type="FunCoup" id="A0A673CFQ6">
        <property type="interactions" value="2084"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055685.1">
        <property type="protein sequence ID" value="ENSSORP00005054411.1"/>
        <property type="gene ID" value="ENSSORG00005024373.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411320"/>
    <dbReference type="CTD" id="8106"/>
    <dbReference type="InParanoid" id="A0A673CFQ6"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12550">
        <property type="entry name" value="RRM_II_PABPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000311">
        <property type="entry name" value="polyadenylate-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF16">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="284" mass="30932" checksum="8163B89518B030D7" modified="2020-06-17" version="1">MAEFGNGLAEESLLDSDPGHPELEDPGVGDEEPGLEEGEAAIEDPELEAIKARVREMEEEAEKLKELQNEVEKQMNLSPPPVGPVIMSIEEKMEADGRSIYVGNVDYGATAEELEAHFHGCGSVNRVTILCDKYTGHPKGFAYIEFADKESVRTAMALDESLFRGRQIKVGAKRTNRPGISTTDRGFPRARFRSRGGSFSSRARYYSGYTPPRGRGRAFRFQDQWRLTTPPQVAPAPPTVSAASLSLSAPAMHTHPILSVWGGGGGGQGDHRPAAGGIYYNSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFR4</accession>
    <name>A0A673CFR4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">kank3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029987244.1">
        <property type="nucleotide sequence ID" value="XM_030131384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CFR4"/>
    <dbReference type="Ensembl" id="ENSSORT00005052789.1">
        <property type="protein sequence ID" value="ENSSORP00005051557.1"/>
        <property type="gene ID" value="ENSSORG00005023284.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417457"/>
    <dbReference type="CTD" id="256949"/>
    <dbReference type="InParanoid" id="A0A673CFR4"/>
    <dbReference type="OrthoDB" id="5406014at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000017">
        <property type="entry name" value="KN motif and ankyrin repeat domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047184">
        <property type="entry name" value="KANK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021939">
        <property type="entry name" value="KN_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168:SF23">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12075">
        <property type="entry name" value="KN_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="800"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="872"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="905"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="60"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="278"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="588"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="969"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="433"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="280"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="649"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="983"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="992" mass="107749" checksum="C99DB0802C8FEB56" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CGF4</accession>
    <name>A0A673CGF4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Olfactomedin 3a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">olfm3a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CGF4"/>
    <dbReference type="Ensembl" id="ENSSORT00005053020.1">
        <property type="protein sequence ID" value="ENSSORP00005051787.1"/>
        <property type="gene ID" value="ENSSORG00005023379.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CGF4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022082">
        <property type="entry name" value="Noelin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF90">
        <property type="entry name" value="NOELIN-3 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12308">
        <property type="entry name" value="Noelin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="11">
        <location>
            <begin position="199"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="60"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="200"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="459" mass="52983" checksum="067D6DE597BBFCF1" modified="2020-06-17" version="1">YIYLFLSLNPSISISISLFISISSFTIGPKEGWQVYSSAQDADGRCICTVVAPEQSLCSRDAKSRQLRQLLEKVQNMSQSIEVLNLRTQRDFQYVMKMENQMKGLRTKFRQIEDDRKSIMARNFQELKDKMDELKPLIPVLEQYKMDAALISQFKEEIRNLSAVLTGIQEELGAYDYDELYQRVLRLDSRLRSCMGKLTCGKLMKITGPVTIKTSGTRFGAWMTDPLASTRNNRVWYMDSYTNSKIVREYKSMDDFVSGVVSRTYSLPFKWEGTNHIVYNGSLYYNKYQSNIIVKYSFDSGSVLAQRALEFAGFHNMYPYTWGGYSDIDVMADELGMWVVYATNQNAGNVVISQIDPDTLQVLKTWNTEYSKRNAGESFMICGTLYITNSHLSGAKVYYAYSTKTSTYEYIDIPFHNQYFHISMLDYNARERALYAWNNGHQVIFNVTLFHVIKTDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CM33</accession>
    <name>A0A673CM33_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="17">ATP-PFK</shortName>
            <shortName evidence="17">Phosphofructokinase</shortName>
            <ecNumber evidence="17">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pfkla</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 18">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="17">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17 18">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homo- and heterotetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A673CM33"/>
    <dbReference type="FunCoup" id="A0A673CM33">
        <property type="interactions" value="1067"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005054347.1">
        <property type="protein sequence ID" value="ENSSORP00005053093.1"/>
        <property type="gene ID" value="ENSSORG00005023083.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CM33"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000001">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000003">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF14">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE, LIVER TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 17" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="67"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="431"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="17">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="17">
        <location>
            <begin position="430"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="127"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="203"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="336"/>
            <end position="339"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="556"/>
            <end position="560"/>
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        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="594"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <begin position="601"/>
            <end position="603"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58579"/>
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        </ligand>
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        <location>
            <position position="657"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58579"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="683"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58579"/>
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        </ligand>
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        <location>
            <begin position="689"/>
            <end position="692"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58579"/>
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        </ligand>
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        <location>
            <position position="760"/>
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            <dbReference type="ChEBI" id="CHEBI:58579"/>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="806" mass="88244" checksum="55D0BA2888254BEC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CNL2</accession>
    <name>A0A673CNL2_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Zgc:112294</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="primary">zgc:112294</name>
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    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
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        <subcellularLocation>
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            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:ciliary transition zone"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:non-motile cilium assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterised_TM-17"/>
    </dbReference>
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        <property type="entry name" value="GEO07735P1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09799">
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        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="103"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="213" mass="24548" checksum="4CE3B62DBFBAAA63" modified="2020-06-17" version="1">MPVFYSSVPENLQLGLAYVGSSVFTNNRTADSDFTREQEELVSHLPLQMLLYFNMFYFPCWWFSAVFMLEVKFSCLAGYYQALLITGIILLTVIEVVRTYLGYVGNLKERVPELAAFWILSFMFQLPVLLFFLTDEGIIILPLERAVHSLYLLFLLAQILASFLALRTMTRKLTLLFHLRQFGKVQCLHHHPGINPVYGLPYHRSVLPMSPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CNV2</accession>
    <name>A0A673CNV2_9TELE</name>
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        <submittedName>
            <fullName evidence="10">Artemin a</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TGF-beta family. GDNF subfamily.</text>
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    <dbReference type="AlphaFoldDB" id="A0A673CNV2"/>
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        <property type="protein sequence ID" value="ENSSORP00005055019.1"/>
        <property type="gene ID" value="ENSSORG00005024605.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030116">
        <property type="term" value="F:glial cell-derived neurotrophic factor receptor binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:receptor tyrosine kinase binding"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043401">
        <property type="entry name" value="GDNF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173">
        <property type="entry name" value="GDNF SUBFAMILY OF TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173:SF8">
        <property type="entry name" value="PERSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="9">
        <location>
            <begin position="10"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009832"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="132" mass="15298" checksum="DDEDACBD4F45C87C" modified="2020-06-17" version="1">MQEEELQHSRWRRSSRDPATSRSTRRNRKKIPKSSRDCHLERKEMRVRDLGLGYDSDETVIFKYCVGTCHSSRKNYDLVLKALIDNGSISGKKISSNPCCRPTRYETVTFMDSQTTWQMIKWLSAANCSCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CVU2</accession>
    <name>A0A673CVU2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="13">ATP-PFK</shortName>
            <shortName evidence="13">Phosphofructokinase</shortName>
            <ecNumber evidence="13">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">pfkmb</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13 14">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13 14">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homo- and heterotetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A673CVU2"/>
    <dbReference type="Ensembl" id="ENSSORT00005058948.1">
        <property type="protein sequence ID" value="ENSSORP00005057629.1"/>
        <property type="gene ID" value="ENSSORG00005023519.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CVU2"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00764">
        <property type="entry name" value="Eukaryotic_PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000001">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000003">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000043">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041914">
        <property type="entry name" value="PFK_vert-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF56">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 13 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="77"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="460"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="13">
        <location>
            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="13">
        <location>
            <begin position="460"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="147"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="177"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="223"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="267"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="357"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="529"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="586"/>
            <end position="590"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="624"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="631"/>
            <end position="633"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="719"/>
            <end position="722"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="793"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="836" mass="91054" checksum="F0C86DC1411BB3A1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CWR2</accession>
    <name>A0A673CWR2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="15">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">cdkal1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 15">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endoplasmic reticulum membrane</location>
            <topology evidence="15">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A673CWR2"/>
    <dbReference type="FunCoup" id="A0A673CWR2">
        <property type="interactions" value="1172"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005058465.1">
        <property type="protein sequence ID" value="ENSSORP00005057162.1"/>
        <property type="gene ID" value="ENSSORG00005025362.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CWR2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000005">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="18">
        <location>
            <begin position="59"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="19">
        <location>
            <begin position="195"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="17">
        <location>
            <begin position="415"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="565" mass="63889" checksum="D2F827A250C58042" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CXW0</accession>
    <name>A0A673CXW0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CXW0"/>
    <dbReference type="Ensembl" id="ENSSORT00005059825.1">
        <property type="protein sequence ID" value="ENSSORP00005058499.1"/>
        <property type="gene ID" value="ENSSORG00005025812.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CXW0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22591">
        <property type="entry name" value="XIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22591:SF3">
        <property type="entry name" value="XIN ACTIN-BINDING REPEAT-CONTAINING 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="906" mass="101274" checksum="CD9AE0F7051D1802" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CY27</accession>
    <name>A0A673CY27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ipo7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CY27"/>
    <dbReference type="FunCoup" id="A0A673CY27">
        <property type="interactions" value="1909"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059056.1">
        <property type="protein sequence ID" value="ENSSORP00005057737.1"/>
        <property type="gene ID" value="ENSSORG00005025534.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CY27"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000053">
        <property type="entry name" value="Importin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
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    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF27">
        <property type="entry name" value="IMPORTIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="881"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="884"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005057737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1049" mass="121036" checksum="52B58C3AB8E1C599" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y2L8</accession>
    <name>A0A672Y2L8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y2L8"/>
    <dbReference type="Ensembl" id="ENSSORT00005000319.1">
        <property type="protein sequence ID" value="ENSSORP00005000297.1"/>
        <property type="gene ID" value="ENSSORG00005000212.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y2L8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08218">
        <property type="entry name" value="STKc_Nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000172">
        <property type="entry name" value="serine/threonine-protein kinase Nek1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="4"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="863" mass="97291" checksum="8C92E5FF53EA89A6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6C9</accession>
    <name>A0A672Y6C9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y6C9"/>
    <dbReference type="FunCoup" id="A0A672Y6C9">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005002204.1">
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        <property type="gene ID" value="ENSSORG00005001298.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y6C9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="110" mass="12756" checksum="70942CE6908D7F30" modified="2020-06-17" version="1">MEVSRRRVLQRHLEPARPLRRCIQVDIEKEGKTQRTPEIISSYILIALSFFPSCHLHFLTGRISYTRDFLIGLACCPEAKRKPEFLPEHPIVLPKAVNSLFYLSYVNSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y6P4</accession>
    <name>A0A672Y6P4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Odorant receptor, family H, subfamily 129, member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y6P4"/>
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        <property type="protein sequence ID" value="ENSSORP00005002282.1"/>
        <property type="gene ID" value="ENSSORG00005001385.1"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF991">
        <property type="entry name" value="ODORANT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
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    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="109"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="31"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="310" mass="35263" checksum="A168275555B78AC7" modified="2020-06-17" version="1">SLISNATSHNTLSMIIKVCVVIPFCCLFLCCIVVMLHIFASHRQFLETSRYILFACMLINDTLQVLSSLLLFHLVVGQVKLAFICCVPLLFISTATFQNTPLILATMSLERYIAIIYPLQRPAAWRSDRIWIIILSLWLISCIFPFIDFSIGKHDPSVNVLFTPVICKTALVNSSPIQQLFKAAVSVLFFVVVAVIILFTYVRILLETRKLRQDRVSVTKALHTVLLHGFQLLLCVLAFTHPITETLIALQSWSPQDLSFFNYFCFMLVPRFLSPLIYGFRDQSLRGYIKKRFLCCSKTVDPLIPKSTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y734</accession>
    <name>A0A672Y734_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y734"/>
    <dbReference type="Ensembl" id="ENSSORT00005002494.1">
        <property type="protein sequence ID" value="ENSSORP00005002432.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:G-protein alpha-subunit binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="29"/>
        </location>
    </feature>
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        <location>
            <begin position="392"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="708"/>
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        </location>
    </feature>
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        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="870" mass="101432" checksum="1E0784526A6301C6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9M2</accession>
    <name>A0A672Y9M2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSSORG00005002078.1"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:response to muscle activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Perm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47282">
        <property type="entry name" value="PGC-1 AND ERR-INDUCED REGULATOR IN MUSCLE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47282:SF1">
        <property type="entry name" value="PGC-1 AND ERR-INDUCED REGULATOR IN MUSCLE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025684759" evidence="1">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="60" mass="6422" checksum="5812710E63916599" modified="2020-06-17" version="1">MCLVCIAFASWVLKSSDPEATDTWKAVTHIEILCSSASKCECSVSNPVPAAVCEEETSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAQ9</accession>
    <name>A0A672YAQ9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YAQ9"/>
    <dbReference type="Ensembl" id="ENSSORT00005000221.1">
        <property type="protein sequence ID" value="ENSSORP00005000205.1"/>
        <property type="gene ID" value="ENSSORG00005000157.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAQ9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23971">
        <property type="entry name" value="GT29_ST8SIA_mono"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000001">
        <property type="entry name" value="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF50">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE 8F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="127"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="340" mass="38175" checksum="4311B5150EAD1E5F" modified="2020-06-17" version="1">PVRPHPQDASSLSTNTEMVYIYQCHFVNTSYLLPLLAFKNCTERQSVGVSCRYLPDIIELYILKPIKSQLKTKCNGIDKAIITQANTPVGSKLVYDGERNKVYQVNSELFKTFVKESPFSNKTWDTCAVVGSGGILANSSCGKAIDSAQFVIRCNLPPVENEFKAHVGSKTEIVTANPSILIEKYGSLSGHRRPFVESLHKYGNSMLLLPAFSYTVNTPVCLRALYTIEDFEIPIQTVFFNPKYLQSLDEFWRTQGLKAIRPSTGLMMVSLALEICSNVHLYGFWPFSNHPHGFQPLNNHYYDNKPPVKGVHAMPVEFNLLLQLHSQGVLRVHLGQCGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YDB6</accession>
    <name>A0A672YDB6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transcription factor Sp5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">sp5l</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YDB6"/>
    <dbReference type="Ensembl" id="ENSSORT00005003204.1">
        <property type="protein sequence ID" value="ENSSORP00005003109.1"/>
        <property type="gene ID" value="ENSSORG00005001915.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YDB6"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035118">
        <property type="term" value="P:embryonic pectoral fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045743">
        <property type="term" value="P:positive regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22541">
        <property type="entry name" value="SP5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF28">
        <property type="entry name" value="SP5 TRANSCRIPTION FACTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="255"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="285"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="331"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="361" mass="40316" checksum="AC3008A1CFF93547" modified="2020-06-17" version="1">MAALTIQRTDNFLHTFLQDRTPSSSPEGAPNALSFLATTCSQAWQVGGTMGSDGSQFPYEGTVSSTSGMFQLWSNDMAPTSALSTHQMTFTVPKVQFPGHMQSGLGHHHHPHHHHHELPLTPPAEPPSSYSFDLSPVKVLSSQPQTNSSYYPQHNSVGQNFSSFLQNSSARHHLPGGHMDEGQQWWSLPQTNATPSNHPFSLGRQLVLGHQPQIAALLQGTSKGLLSSTRRCRRCKCPNCQANGGGLEFGKKRLHICHIPECGKVYKKTSHLKAHLRWHAGERPFICNWLFCGKSFTRSDELQRHLRTHTGEKRFGCQQCGKRFMRSDHLSKHVKTHQTRKSRSGQPSQTTDPLLTNIKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YE08</accession>
    <name>A0A672YE08_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Muscle-specific beta 1 integrin binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YE08"/>
    <dbReference type="Ensembl" id="ENSSORT00005001370.1">
        <property type="protein sequence ID" value="ENSSORP00005001331.1"/>
        <property type="gene ID" value="ENSSORG00005000783.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YE08"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11931" checksum="8EC45A7F7F2AE2F4" modified="2020-06-17" version="1">MDNSVRYDYDINIFIVMVEGFLLYTYEPLINMLNQRYFISIPYEECKKRRCSRNYAVPDPPGLFDGHVWPTYLKHKNTQPWSWRATILHVLDVSLFQHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YEB5</accession>
    <name>A0A672YEB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ythdf3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the YTHDF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029978595.1">
        <property type="nucleotide sequence ID" value="XM_030122735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YEB5"/>
    <dbReference type="SMR" id="A0A672YEB5"/>
    <dbReference type="Ensembl" id="ENSSORT00005002604.1">
        <property type="protein sequence ID" value="ENSSORP00005002540.1"/>
        <property type="gene ID" value="ENSSORG00005001517.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410906"/>
    <dbReference type="CTD" id="253943"/>
    <dbReference type="InParanoid" id="A0A672YEB5"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000001">
        <property type="entry name" value="YTH domain family 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF9">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="7">
        <location>
            <begin position="436"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="243"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="312"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="605" mass="66165" checksum="C4493075B77C8AA4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YF92</accession>
    <name>A0A672YF92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoprotein hormone beta-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Thyrostimulin subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115438845</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a heterodimeric glycoprotein hormone with GPHA2 able to bind and activate the thyroid-stimulating hormone receptor (TSHR), leading to increased cAMP production. Plays a central role in controlling thyroid cell metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer with GPHA2; this heterodimer interacts with thyroid-stimulating hormone receptor (TSHR), and hence stimulates cAMP production.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycoprotein hormones subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030018559.1">
        <property type="nucleotide sequence ID" value="XM_030162699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YF92"/>
    <dbReference type="Ensembl" id="ENSSORT00005003490.1">
        <property type="protein sequence ID" value="ENSSORP00005003390.1"/>
        <property type="gene ID" value="ENSSORG00005002061.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438845"/>
    <dbReference type="InParanoid" id="A0A672YF92"/>
    <dbReference type="OrthoDB" id="10006958at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002155">
        <property type="term" value="P:regulation of thyroid hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00069">
        <property type="entry name" value="GHB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000029">
        <property type="entry name" value="glycoprotein hormone beta-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001545">
        <property type="entry name" value="Gonadotropin_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11515">
        <property type="entry name" value="GLYCOPROTEIN HORMONE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11515:SF14">
        <property type="entry name" value="GLYCOPROTEIN HORMONE BETA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00068">
        <property type="entry name" value="GHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025576915" description="Glycoprotein hormone beta-5" evidence="12">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoprotein hormone subunit beta" evidence="13">
        <location>
            <begin position="34"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="14615" checksum="8FD656DEDE748044" modified="2020-06-17" version="1">MHLHPLSFSFLFILVAGVAVMCVAVTTTLHGFRGCAVREFSFVAQKPGCKGLRITTEACWGRCHTWEKPVPDPPYIHRHHRVCTYSRTRHMTARLPGCQPEVSPLYHYPMALHCHCAVCSTQDTECETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YH53</accession>
    <name>A0A672YH53_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Guanine nucleotide-binding protein G(t) subunit alpha-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gnat2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YH53"/>
    <dbReference type="FunCoup" id="A0A672YH53">
        <property type="interactions" value="1049"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005004025.1">
        <property type="protein sequence ID" value="ENSSORP00005003911.1"/>
        <property type="gene ID" value="ENSSORG00005002287.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YH53"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001580">
        <property type="term" value="P:detection of chemical stimulus involved in sensory perception of bitter taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000720">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000256">
        <property type="entry name" value="Guanine nucleotide-binding protein G(t) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
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        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF68">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
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        <property type="entry name" value="G_alpha"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="39"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <begin position="146"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <begin position="171"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="196"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
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        <location>
            <begin position="265"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="350" mass="39544" checksum="23DF368838A1EB00" modified="2020-06-17" version="1">MGAGASAEDKKSKELEKQLQEDADKDSKTVKLLLLGAGESGKSTIVKQMKILHQGGYTKEEQMEFRAIIYGNILQSALAIIRGMEMLGIDFGSPSGQEDAQKLQNLSDSIEEGTMPSELADVIKKLWGDSGVQAGFERAAEYQLNDSAGYYLNEMDRICKPDYLPTEQDVLRSRVKTTGIIEEQFSCKELHFRMFDVGGQRSERKKWIHCFEGVTCIIFCGALSAYDMVLVEDDEVNRMHESLHLFNSICNHRFFALTSIVLFLNKKDLFEEKIKKVHLSICFPDYDGPNTYDDASNYIKTQFLDLNMKKGVKEIYSHLTCATDTKNVEIVFNAVTDIIIKENLKDCGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YH89</accession>
    <name>A0A672YH89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">uqcrb</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YH89"/>
    <dbReference type="FunCoup" id="A0A672YH89">
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005003930.1"/>
        <property type="gene ID" value="ENSSORG00005002393.1"/>
    </dbReference>
    <dbReference type="CTD" id="7381"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
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        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="QCR7"/>
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        <property type="entry name" value="QCR7_sf"/>
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    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UCR_14kD"/>
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    </dbReference>
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        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="110" mass="13302" checksum="58CD1ADEA5D677C6" modified="2020-06-17" version="1">MASRAPVASGRFLLGVRKWYYNMCGFNKLGLMRDDTLYEDTDVKEAVRRLPENLFNDRMFRIKRAMDLSMKQQILPKDQWTKFDEDVAYLTPYLQEVIRERKEKEEWTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIZ0</accession>
    <name>A0A672YIZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YIZ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005004670.1">
        <property type="protein sequence ID" value="ENSSORP00005004536.1"/>
        <property type="gene ID" value="ENSSORG00005002751.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YIZ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003074">
        <property type="entry name" value="Immunoglobulin heavy variable 11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="123" mass="14888" checksum="F57586F5EAAF633C" modified="2020-06-17" version="1">QTLTESGSAVKSPGESHRLTCTTSGFTFSSHWMHWVRQAPGKGLEWIAAVDYGGSSKYYSQSVQGRFTISRDNGRNQLYLQMNNLKTEDSAVYYCALNAYYYYYYYYYYYYYYYYYYYYYYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ10</accession>
    <name>A0A672YJ10_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="99" mass="11186" checksum="DFFF85FA4A9B7BB3" modified="2020-06-17" version="1">GQTLTESGPAVKRPGESHRLTCTTSGFTFSSYDMNWIRQAPGKGLEWIAYIATTTTYYSQSVQGRFTISRDNSRNQLYLQMNSLKTEDSAVYYCARVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YMF3</accession>
    <name>A0A672YMF3_9TELE</name>
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        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">vsig10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030000040.1">
        <property type="nucleotide sequence ID" value="XM_030144180.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A672YMF3"/>
    <dbReference type="FunCoup" id="A0A672YMF3">
        <property type="interactions" value="1006"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005005749.1"/>
        <property type="gene ID" value="ENSSORG00005003478.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426183"/>
    <dbReference type="CTD" id="54621"/>
    <dbReference type="InParanoid" id="A0A672YMF3"/>
    <dbReference type="OrthoDB" id="9043395at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="Immunoglobulin_dom"/>
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        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF14">
        <property type="entry name" value="CELL ADHESION MOLECULE DSCAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025383298" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="16"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
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            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
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            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
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            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="510"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="543"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="543" mass="58161" checksum="57620022CB6088C3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YN23</accession>
    <name>A0A672YN23_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YN23"/>
    <dbReference type="Ensembl" id="ENSSORT00005006235.1">
        <property type="protein sequence ID" value="ENSSORP00005005985.1"/>
        <property type="gene ID" value="ENSSORG00005003568.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YN23"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="59"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="181" mass="19806" checksum="1E843BC8C7E93871" modified="2020-06-17" version="1">MAFTMLRPVSTHPFSYPVDLTLMSDDEEGNRSESDGSTDQGYGGGCYGIPGETGGVVVRQRNAANARERHRTQNVNTAFTALRTLIPTEPVDRKLSKIETLRLASSYISHLANVLVVGDGKDDGQPCLSAVYKEVKGRGDGKQPRSICTFCLSNQRKGVTIRVCLNMRKHQISSIKSVFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNT8</accession>
    <name>A0A672YNT8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome P450 20A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115430983</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030007066.1">
        <property type="nucleotide sequence ID" value="XM_030151206.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030007074.1">
        <property type="nucleotide sequence ID" value="XM_030151214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YNT8"/>
    <dbReference type="FunCoup" id="A0A672YNT8">
        <property type="interactions" value="1066"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005006496.1">
        <property type="protein sequence ID" value="ENSSORP00005006244.1"/>
        <property type="gene ID" value="ENSSORG00005003585.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430983"/>
    <dbReference type="CTD" id="57404"/>
    <dbReference type="InParanoid" id="A0A672YNT8"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052666">
        <property type="entry name" value="CYP450_20A1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24280">
        <property type="entry name" value="CYTOCHROME P450 20A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24280:SF4">
        <property type="entry name" value="CYTOCHROME P450 20A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="3">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="462" mass="52000" checksum="3BACB19D772DCAEA" modified="2020-06-17" version="1">MLDFAIFAVTFVIILVGAVLYLYPSSRRASGIPGFNPTDEKDGNLQDIVNRGSLHEFLVSLHQEFGSVASFWFGGRPVVSLGSVHQLQQHINPNHSTDSFETMLKSLLGYQSGIGGGANESVIRKKVYENAINTMLKNNFSLVLKLVDELVGKWRSFPEDQHTPLCAHLLGLALKAVTQLALGERFRDDTEVISFRKNHDAIWSEIGKGYLDGSLEKSSSRKERYEKALSEMESMLVSVEKERKAQRRQTAFVDSLLQSSLTERQIMEDCMVFTLAGSVITANLCIWALHFLSTSEEVQDKLYQELEDVLGSDPISLDKIAQLTYCQQVLNETVRTAKLTPVAARLQEVEGKVDQHVIPKETLVIYALGVVLQDSETWRTPYRFDPDRFKEESARKSFCLLGFSGNQTCPELRFAYTVATVLLSTLVRQLKLHQLKGQVMEVRSELVSTPKDETWITVSRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQJ5</accession>
    <name>A0A672YQJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kringle-containing protein marking the eye and the nose</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">kremen1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000127.1">
        <property type="nucleotide sequence ID" value="XM_030144267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YQJ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005007072.1">
        <property type="protein sequence ID" value="ENSSORP00005006810.1"/>
        <property type="gene ID" value="ENSSORG00005003956.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426269"/>
    <dbReference type="CTD" id="83999"/>
    <dbReference type="InParanoid" id="A0A672YQJ5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000006">
        <property type="entry name" value="Kremen protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF26">
        <property type="entry name" value="KRINGLE-CONTAINING PROTEIN MARKING THE EYE AND THE NOSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 11" id="KW-0420">Kringle</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025591347" description="Kringle-containing protein marking the eye and the nose" evidence="14">
        <location>
            <begin position="21"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="16">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="17">
        <location>
            <begin position="112"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="15">
        <location>
            <begin position="210"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="322"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="460" mass="50670" checksum="A6B03A6913523D01" modified="2020-06-17" version="1">MDSWTVTSALLLAGLISSAAFSHFDTECYTANGEDYRGFQNQTSLHGGKPCLFWNETFQHPYNTLKYPNGEGGLGSHNYCRNPDGDVQPWCYIADHEDGIYWRYCDIPTCQMPGNLGCFKDSGDPPTLSGTSETSNKLTIQNCISFCRKQRYKLAGMESGYACFCGNEVDLRDHGESPGMECNHVCFGNHNQPCGGDGWVIIFDTRVGACGGNYSAPSGVIYSPDFPDKYNPGRVCYWTIQVPGSSAILFNFTFFDISDQTDMVELLDGYTNQVVARFDWRSPPRELVNITGDFVILYFYSDRTNQAQGFALLYQALRGPDTRTTEAESEEEGEDVSSTTGPQLVGSVTEKSNSTSNGRSSQILYVITSSPGKPEHNMPVWTIYALAALLILTVIAMVAKLLLHITVKSPNIPTVSGSDSCSQSTASSEPWIILYRPSTISLFKKKLKNHHGDLSPLVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQR4</accession>
    <name>A0A672YQR4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G protein-coupled receptor 101</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YQR4"/>
    <dbReference type="FunCoup" id="A0A672YQR4">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007196.1">
        <property type="protein sequence ID" value="ENSSORP00005006934.1"/>
        <property type="gene ID" value="ENSSORG00005004011.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YQR4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF15">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 101-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="141"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
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        </location>
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            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
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        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="368" mass="41448" checksum="53F226805ADD5C7C" modified="2020-06-17" version="1">MGTNFTDGLSSSSEDRLLWSSTANSVMKMVLISLIVCVSLFGNVVVLLVFQRKPQLLHVANRFVLNLLLADLLQTILVMPFAIAATVPGVWPLDSRLCQALVVLMHLFAFAGVNTIIVVSVDRYLAIIHPLSYPTRMTPHLGTNLIICTWVLSFLQSTPPLYGWGTIDFDRHHNVCSVVWSSSLSYSAVVSTFSFWLPVLIMLGCYWMVFRAARRQNALVHPIQTQSYSQPCPQDFQGPTSPQRQPQPQPQQACSPEGPYSARGYPVRVRHRRFHYHCKAARVVFVIMASYILSMGPYSILNTISMSARAAVPPWFVLCGLVLFFLQCCLHPYIYGYMHRSVRKEFLALLCSLFCKQSRTSQSSAYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRN4</accession>
    <name>A0A672YRN4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YRN4"/>
    <dbReference type="Ensembl" id="ENSSORT00005007476.1">
        <property type="protein sequence ID" value="ENSSORP00005007205.1"/>
        <property type="gene ID" value="ENSSORG00005004133.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YRN4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="146" mass="17039" checksum="7984D371D0718140" modified="2020-06-17" version="1">MLLPFSCGLCVFTVSLLIIKLKQVRTMVWLQILSHLAAFASRAFLFLILAFSTPPLFFLFSMFQTVNTAEHPQPTEHRWYMLHRYRTELLTVCKDMLRQVTVCPQKKNRKKTNSLLVEGVWPRNSSCRLRGQLSAMTEHRPQKINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YRZ0</accession>
    <name>A0A672YRZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease V</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YRZ0"/>
    <dbReference type="Ensembl" id="ENSSORT00005007624.1">
        <property type="protein sequence ID" value="ENSSORP00005007346.1"/>
        <property type="gene ID" value="ENSSORG00005004202.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YRZ0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13811" checksum="0BCE923318A8C43D" modified="2020-06-17" version="1">TELRARPHIESFSLVSEQQRLRQQVVEEDTEAWQRSPHLSGLERVGGVDLSFIKGDHVNACAQLVVLSYPHLEVSSGRGRTLTTSATFTIIEMTIIKNDYLLTLKLFIFFLAKQCKIYFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSR6</accession>
    <name>A0A672YSR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115416693</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YSR6"/>
    <dbReference type="Ensembl" id="ENSSORT00005007899.1">
        <property type="protein sequence ID" value="ENSSORP00005007619.1"/>
        <property type="gene ID" value="ENSSORG00005004246.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YSR6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="75"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="274"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="476" mass="53037" checksum="0AEBD532A4BB2B56" modified="2020-06-17" version="1">FIQKQSQPSNKCDLGQSIHFCPFLSLQRECISMHVGQAGAQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDLVLDRTRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVADITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNSAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGTDSIDQEDDEGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YT13</accession>
    <name>A0A672YT13_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Kinesin family member 26Aa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115414894</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005007928.1">
        <property type="protein sequence ID" value="ENSSORP00005007647.1"/>
        <property type="gene ID" value="ENSSORG00005004337.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YT13"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000015">
        <property type="entry name" value="Kinesin family member 26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21608:SF5">
        <property type="entry name" value="KINESIN FAMILY MEMBER 26AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21608">
        <property type="entry name" value="KINESIN-LIKE PROTEIN CG14535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="53"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="400"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="525"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="650"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="773"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="839"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1031"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1102"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1163"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1206"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1371"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1497"/>
            <end position="1524"/>
        </location>
    </feature>
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        <location>
            <begin position="407"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="790"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="871"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="893"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="946"/>
            <end position="964"/>
        </location>
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        <location>
            <begin position="981"/>
            <end position="993"/>
        </location>
    </feature>
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        <location>
            <begin position="1033"/>
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        <location>
            <begin position="1065"/>
            <end position="1077"/>
        </location>
    </feature>
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        <location>
            <begin position="1222"/>
            <end position="1236"/>
        </location>
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        <location>
            <begin position="1249"/>
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        </location>
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        <location>
            <begin position="1301"/>
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        <location>
            <begin position="148"/>
            <end position="155"/>
        </location>
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            <name>ATP</name>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1596" mass="170887" checksum="B93CB57CABB23928" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YV23</accession>
    <name>A0A672YV23_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">Leucine rich repeat containing 69</fullName>
        </recommendedName>
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        <name evidence="4" type="primary">lrrc69</name>
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    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
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            <begin position="1"/>
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            <begin position="24"/>
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        </location>
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        </source>
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            <dbReference type="Ensembl" id="ENSSORP00005008327.1"/>
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            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="35045" checksum="86D93964DCE16CEB" modified="2020-06-17" version="1">MPRVCKAVIRIFDYILLFFLCNACKLTELNLGNNSLKEVPAVLAHLESLKKLYLYSNQITLVPPEVIGCLPNLVILNLNHNHIQRLPAEVKSLVKLQHLSVMDNQLEEVPAELGHLTELTEMNFTFNNVSSLPQQLYRCRELTKLYLARNKLSHLPEGIGALAKLQVLDVAGNKLSMFPFYLLRLKELHCEGNRFIRCEPMSAVQHVEVLTLKELVARFVLQEDRNRLSLVHRMLPHYPPLTVLLASGSCCALCRGPFLTTWLECVHFISLRKVMHMKSSLIIPVRALLCSYKCFNTSGHAYYGVASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YW03</accession>
    <name>A0A672YW03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytosolic beta-glucosidase</fullName>
            <ecNumber evidence="5">3.2.1.21</ecNumber>
            <ecNumber evidence="4">3.2.1.45</ecNumber>
            <ecNumber evidence="3">3.2.1.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cytosolic galactosylceramidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytosolic glucosylceramidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Cytosolic glycosylceramidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">lctlb</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a beta-D-galactosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:14297"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18390"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.46"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:14298"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:13269"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.45"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:13270"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a beta-D-xylosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-xyloside + an N-acylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:70239"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:189067"/>
            <dbReference type="ChEBI" id="CHEBI:189068"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:70240"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:70241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>beta-D-galactosyl-(1&lt;-&gt;1')-N-octadecanoylsphing-4-enine + H2O = N-octadecanoylsphing-4-enine + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:59292"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:72961"/>
            <dbReference type="ChEBI" id="CHEBI:84720"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:59293"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>beta-D-galactosyl-(1&lt;-&gt;1)-sphing-4-enine + H2O = sphing-4-enine + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:43908"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="ChEBI" id="CHEBI:57934"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:43909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>beta-D-glucosyl-(1&lt;-&gt;1)-N-octadecanoylsphing-4-enine + H2O = N-octadecanoylsphing-4-enine + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:59284"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:72961"/>
            <dbReference type="ChEBI" id="CHEBI:84719"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:59285"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>beta-D-glucosyl-(1&lt;-&gt;1)-sphing-4-enine + H2O = sphing-4-enine + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:59288"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="ChEBI" id="CHEBI:83992"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:59289"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="5"/>
    <dbReference type="EC" id="3.2.1.45" evidence="4"/>
    <dbReference type="EC" id="3.2.1.46" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A672YW03"/>
    <dbReference type="FunCoup" id="A0A672YW03">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005008917.1">
        <property type="protein sequence ID" value="ENSSORP00005008617.1"/>
        <property type="gene ID" value="ENSSORG00005004769.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YW03"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004336">
        <property type="term" value="F:galactosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004348">
        <property type="term" value="F:glucosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000011">
        <property type="entry name" value="Cytosolic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF336">
        <property type="entry name" value="LACTASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 20" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0732">Signal</keyword>
    <keyword evidence="21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025362102" description="Cytosolic beta-glucosidase" evidence="22">
        <location>
            <begin position="21"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="558"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="19">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="583" mass="67493" checksum="1EC8F653E88E5660" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYG7</accession>
    <name>A0A672YYG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prolactin receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">prlra</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YYG7"/>
    <dbReference type="Ensembl" id="ENSSORT00005009972.1">
        <property type="protein sequence ID" value="ENSSORP00005009648.1"/>
        <property type="gene ID" value="ENSSORG00005005272.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYG7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000287">
        <property type="entry name" value="Prolactin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000358">
        <property type="entry name" value="Prolactin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015152">
        <property type="entry name" value="Growth/epo_recpt_lig-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF46">
        <property type="entry name" value="INTERLEUKIN 5 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09067">
        <property type="entry name" value="EpoR_lig-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="101"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="601" mass="67891" checksum="D8B796C00A1207DA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZW2</accession>
    <name>A0A672YZW2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SHC-transforming protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Src homology 2 domain-containing-transforming protein C1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">shc1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YZW2"/>
    <dbReference type="Ensembl" id="ENSSORT00005010686.1">
        <property type="protein sequence ID" value="ENSSORP00005010351.1"/>
        <property type="gene ID" value="ENSSORG00005005594.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZW2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01209">
        <property type="entry name" value="PTB_Shc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09925">
        <property type="entry name" value="SH2_SHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000036">
        <property type="entry name" value="SHC-transforming protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000005">
        <property type="entry name" value="SHC-transforming protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051235">
        <property type="entry name" value="CEP152/SHC-Transforming"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006019">
        <property type="entry name" value="PID_Shc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035676">
        <property type="entry name" value="SHC_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337">
        <property type="entry name" value="SHC TRANSFORMING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337:SF2">
        <property type="entry name" value="SHC-TRANSFORMING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00629">
        <property type="entry name" value="SHCPIDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="PID" evidence="9">
        <location>
            <begin position="192"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="10">
        <location>
            <begin position="506"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="95"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="601" mass="65023" checksum="105DBCABF8C439CA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2C0</accession>
    <name>A0A672Z2C0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serotonin N-acetyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aralkylamine N-acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">aanat1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-arylethylamine + acetyl-CoA = an N-acetyl-2-arylethylamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55469"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:77827"/>
            <dbReference type="EC" id="2.3.1.87"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the acetyltransferase family. AANAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.87" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z2C0"/>
    <dbReference type="FunCoup" id="A0A672Z2C0">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011237.1">
        <property type="protein sequence ID" value="ENSSORP00005010864.1"/>
        <property type="gene ID" value="ENSSORG00005005887.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2C0"/>
    <dbReference type="OrthoDB" id="30840at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030187">
        <property type="term" value="P:melatonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000021">
        <property type="entry name" value="Serotonin N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051635">
        <property type="entry name" value="SNAT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10908:SF4">
        <property type="entry name" value="ARYLALKYLAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10908">
        <property type="entry name" value="SEROTONIN N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0471">Melatonin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="30"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="202" mass="23253" checksum="CC5CC7DFF98412A1" modified="2020-06-17" version="1">MSVVSAVPFMRPLHMRSPVPRGRRHTLPASEFRSLSPEDAISVFEIEREAFISVSGECPLYLDEVRHFLSLCPELSLGWFEEGRLVAFIIGSLWDQDRLTEDALTLHKPHGTTVHIHVLAVHRTFRQQGKGSILMWRYLQYLRCMPCVRRAVLMCEDFLVPFYRKSGFKVQGPSNITVGPLTFIEMFYPVRGHAFMRRNSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z304</accession>
    <name>A0A672Z304_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P2Y purinoceptor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">p2ry2.1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z304"/>
    <dbReference type="Ensembl" id="ENSSORT00005011986.1">
        <property type="protein sequence ID" value="ENSSORP00005011601.1"/>
        <property type="gene ID" value="ENSSORG00005006198.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z304"/>
    <dbReference type="OrthoDB" id="10018446at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031686">
        <property type="term" value="F:A1 adenosine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045030">
        <property type="term" value="F:G protein-coupled UTP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF17">
        <property type="entry name" value="P2Y PURINOCEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="348" mass="39841" checksum="A56B8A2EDDAF82D7" modified="2020-06-17" version="1">MATSKIEDSQPSNGSEYDCQFQEDFKYILLPVSYALVFVIGLALNAAALYVIVFRTKRWKPSTIYMLNLTVCDTLYILTLPFLIYYYADENDWPFSEPFCKIIRFLFYANLYGSILFLCCISLHRFMGICYPVRSLNWVSTRRAKLVSVAVWTCVLTCQAPVLYFSRTRDTATERICYDTTSPDLFDDFLVYSSIVSVLMFALPFMVVMVCYGLMVRKLLEPCWGSEGRSGGQRGGRATQRYKQKSVQMIIIVLAAFMLCFLPFHLTRSLYYSFRYLQQVNPAQVSCSLLEASSVAYKVTRPFASANSCVDPILYFLGGQDIRCNLTKKNKTLSSKPQKMTKDLTTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z5D7</accession>
    <name>A0A672Z5D7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Semaphorin-7A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115417235</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987109.1">
        <property type="nucleotide sequence ID" value="XM_030131249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z5D7"/>
    <dbReference type="Ensembl" id="ENSSORT00005012406.1">
        <property type="protein sequence ID" value="ENSSORP00005012014.1"/>
        <property type="gene ID" value="ENSSORG00005006373.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417235"/>
    <dbReference type="InParanoid" id="A0A672Z5D7"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043931">
        <property type="term" value="P:ossification involved in bone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001170">
        <property type="entry name" value="Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (Semaphorin) 3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF144">
        <property type="entry name" value="SEMAPHORIN-7A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025347166" evidence="7">
        <location>
            <begin position="22"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="8">
        <location>
            <begin position="1"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="625" mass="70586" checksum="4A75A39885F764D6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9B0</accession>
    <name>A0A672Z9B0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z9B0"/>
    <dbReference type="Ensembl" id="ENSSORT00005014364.1">
        <property type="protein sequence ID" value="ENSSORP00005013946.1"/>
        <property type="gene ID" value="ENSSORG00005007145.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z9B0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002843">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000290">
        <property type="entry name" value="Zinc finger protein 697 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="508" mass="57368" checksum="36D8769CBC21094D" modified="2020-06-17" version="1">MKAESSWEGKDLTTPGRTATQATIKTESSLEDIDHPVPATTLIQAKDIKTEICWEDDNIPTPANPVDQVNDIKTESSREDKDLPTPTKTLTQVNTCKTPKRTRTREKRHPCVQCGKSFTSTGELKRHQRIHTGERPYNCYVCGKAFIKADHLKSHQRTHTGEKPYRCDVCGKAFTKISHLKTHQHTHTGEKPYRCDVCGKAFTQSPTLKIHLRSHTGEKPYTCLQCGKVFTNRGNLRIHQRTHTGEKPYSCDFCGKGFIKASHLRTHQRIHTGEKPYICDQCGKAFTAAGNLRKHKRIHTGEKPHSCDQCGKAFTKAGDLRTHQRIHTGEKPYSCPFCGKGFIKASYLKTHQRIHTGEKPYICGQCGKAFRSSSEYSKHQLRHTKEMVIPCEVCGKIFYSASTLRIHQRTHTGEKPYQCGLCERSFTTASQRAEHKCTYKGVHQNNMKTEELGPQDYVAAVAEVCNPGRDRQGKPWPNSASCDPETGAISNKRLVFADSYICPLTLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9D4</accession>
    <name>A0A672Z9D4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTPase IMAP family member 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115429466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004793.1">
        <property type="nucleotide sequence ID" value="XM_030148933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z9D4"/>
    <dbReference type="Ensembl" id="ENSSORT00005013551.1">
        <property type="protein sequence ID" value="ENSSORP00005013143.1"/>
        <property type="gene ID" value="ENSSORG00005006836.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429466"/>
    <dbReference type="InParanoid" id="A0A672Z9D4"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF112">
        <property type="entry name" value="SI:CH211-113E8.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="32"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="236"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="337" mass="37507" checksum="F49F0C020B3F4258" modified="2020-06-17" version="1">MAEAIPLREKKQRTLPRSDGCGLNNFDALDLSGGLRIVLVGKTGSGKSATGNTILGRAAFKEDPSPVSVTKHCETQSGEVDGTMVQVIDTPGLFDTAITEEELKTRIEECVKMSVPGPHAFLLVIRLGVRFTEEERNAVKWIQDNFGDDASMYTIMLFTCKDQAKADNALKECKELRRLSITFGRRYHAFNNNDADDRVQVSELITMIKEMVQDNGGKHYTNEMYEKAQRKLREEEERKKQEEEEKKEEERKMWDAEREKQEKEREKEKKVKRKNIRVASAAAIVLVLAGVVIAVGADNTVALALGAPALVLGVLCGLAAISIWKGITCKPKANVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZC77</accession>
    <name>A0A672ZC77_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZC77"/>
    <dbReference type="Ensembl" id="ENSSORT00005014940.1">
        <property type="protein sequence ID" value="ENSSORP00005014515.1"/>
        <property type="gene ID" value="ENSSORG00005007426.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZC77"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="133" mass="14785" checksum="82A8C8DC612EE726" modified="2020-06-17" version="1">MVRELCHSLMSCCLADCLPVYDLFPSLTSLCFSLVGVPTQNAEPYRGFASLVRRTNPKRISLVTVLKILLFIPIIYLSVQSPNRFSKDSVKIAYVASLLEGPPLSYFNALFEQGSPISVAPVCVPEEREKDFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZCA9</accession>
    <name>A0A672ZCA9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Apoptosis-inducing factor 1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Programmed cell death protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">aifm1</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>A + NADH + H(+) = AH2 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:11356"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Monomer (oxidized form). Homodimer (reduced form). Upon reduction with NADH, undergoes dimerization and forms tight, long-lived FADH2-NAD charge transfer complexes (CTC) resistant to oxidation. Also dimerizes with isoform 3 preventing its release from mitochondria. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Interacts with PRELID1. Interacts with CHCHD4; the interaction increases in presence of NADH. Interacts with processed form of PARP1 (Poly [ADP-ribose] polymerase 1, processed C-terminus); interaction is mediated with poly-ADP-ribose chains attached to PARP1, promoting translocation into the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZCA9"/>
    <dbReference type="Ensembl" id="ENSSORT00005015385.1">
        <property type="protein sequence ID" value="ENSSORP00005014951.1"/>
        <property type="gene ID" value="ENSSORG00005004158.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZCA9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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        <source>
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</entry>
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    <accession>A0A672ZES3</accession>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50259"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005015485.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="864" mass="96246" checksum="72653779BC3A72CC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZGE5</accession>
    <name>A0A672ZGE5_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">mRNA m(6)A methyltransferase</fullName>
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        <name evidence="8" type="primary">mettl3</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55584"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.348"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.348" evidence="1"/>
    <dbReference type="RefSeq" id="XP_030018809.1">
        <property type="nucleotide sequence ID" value="XM_030162949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZGE5"/>
    <dbReference type="SMR" id="A0A672ZGE5"/>
    <dbReference type="FunCoup" id="A0A672ZGE5">
        <property type="interactions" value="627"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016859.1">
        <property type="protein sequence ID" value="ENSSORP00005016351.1"/>
        <property type="gene ID" value="ENSSORG00005008295.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439052"/>
    <dbReference type="CTD" id="56339"/>
    <dbReference type="InParanoid" id="A0A672ZGE5"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025848">
        <property type="entry name" value="MT-A70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51563">
        <property type="entry name" value="SAM_MTA70L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="591" mass="64957" checksum="9845538EE51F136E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJ33</accession>
    <name>A0A672ZJ33_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BICD family-like cargo adapter 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Bicaudal D-related protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 64B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">bicdl2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZJ33"/>
    <dbReference type="FunCoup" id="A0A672ZJ33">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016998.1">
        <property type="protein sequence ID" value="ENSSORP00005016488.1"/>
        <property type="gene ID" value="ENSSORG00005008369.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJ33"/>
    <dbReference type="OrthoDB" id="9451547at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055107">
        <property type="term" value="P:Golgi to secretory granule transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF11">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="395"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="187"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="303"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="395"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="438" mass="50286" checksum="8B26C83108270DDE" modified="2020-06-17" version="1">MFSPRKNSLPSPSLEDSFFPLSSSSSVSLSFALPSSSSPSGSIDSSGGIETDLILAAELGQALLEKNEELAASLDQRERELEVLQQEKHVLQRKLDMNELTSGQREAELMADIATVKAELERYHNHGRDRRRDESEQLTQLANHNQRLVEQLAEAVTLEHSLRTELRSLREEMDESSFSRNISYTQLENIQAENRVLLERLTHMETQLKASQDDTDRLRIEREGLRERVSDLQVKLKEKEAEIEQEQGVVFELRTVNRSLQQKALSLGEESILDSTHIQPLSLLSEIQQSQAKEALLAHSEVLQARDEEIKTLKEELQSKEEELESLREEIKPFRSSPDKPNYSYLENELATVRQEKESLTNQLLNTIKHKVALSQELDAWQEDMRLVINQQVQQREEERQKEQQREKDKTTGLQRSKSLKVKGEGGKGFFSSLFRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLI5</accession>
    <name>A0A672ZLI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin, epsilon 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tube1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029983822.1">
        <property type="nucleotide sequence ID" value="XM_030127962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZLI5"/>
    <dbReference type="Ensembl" id="ENSSORT00005017840.1">
        <property type="protein sequence ID" value="ENSSORP00005017322.1"/>
        <property type="gene ID" value="ENSSORG00005008672.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115414698"/>
    <dbReference type="CTD" id="51175"/>
    <dbReference type="InParanoid" id="A0A672ZLI5"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02190">
        <property type="entry name" value="epsilon_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000007">
        <property type="entry name" value="tubulin epsilon chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000017">
        <property type="entry name" value="Tubulin epsilon chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004057">
        <property type="entry name" value="Epsilon_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01519">
        <property type="entry name" value="EPSLNTUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="9">
        <location>
            <begin position="54"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="10">
        <location>
            <begin position="278"/>
            <end position="413"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="52469" checksum="2248535313575ED7" modified="2020-06-17" version="1">MTQSVVVQVGQCGNQVGCRFWDLALREHAHVNKKGLYDEALSSFFRNVDSRKSDGGAVGVGGRIQHLKARAVLVDMEEGVVKEILQGPLREVFDSTQLLTDVSGSGNNWAVGHMMYGSAYREQIVEKLRKAAEHCDCLQCFFLIHSMGGGTGSGLGTRVLGLLEEEFPEVCRIVTSIYPSAEDDVITSPYNSVLAMRELTEHADCVLPVENQSLVDIVNKIKHMSYSGKAGSVIKTDSSIISGRGGVSGAEKPFDAMNNIVANLLLNITSSARFEGSLNMDLNEIAMNLVPFPRLHYLVPSLTPLYTLADVSVLSRRLDQMFSDAFSKDHQLIRADPKHSLYLACALMVRGNVQVSDLRRNIERLRPSLPFVSWNQEGWKTGLCSVPPVGHSHSLLALANNTCVKPTFVDLRERFAKLYRKKAHLHHYLHVEGMEQSFFSEAMASLSSLIEEYHQLDATKRTLVPDAPRLSIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLU1</accession>
    <name>A0A672ZLU1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase TRIM39-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115434959</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030012970.1">
        <property type="nucleotide sequence ID" value="XM_030157110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZLU1"/>
    <dbReference type="FunCoup" id="A0A672ZLU1">
        <property type="interactions" value="681"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018286.1">
        <property type="protein sequence ID" value="ENSSORP00005017764.1"/>
        <property type="gene ID" value="ENSSORG00005008850.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434959"/>
    <dbReference type="InParanoid" id="A0A672ZLU1"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="Butyrophylin_SPRY"/>
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    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
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        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
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    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
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        <property type="entry name" value="B-box zinc-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="348"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="247"/>
            <end position="296"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="546" mass="62256" checksum="27B67458BD06097C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZMU5</accession>
    <name>A0A672ZMU5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RIMS binding protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RIMBP family.</text>
    </comment>
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    </dbReference>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIMS-binding protein 2 isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000072">
        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="RIMS-binding protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057884">
        <property type="entry name" value="FN3_RIM-BP1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035753">
        <property type="entry name" value="RIM-BP_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035755">
        <property type="entry name" value="RIM-BP_SH3_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040325">
        <property type="entry name" value="RIMBP1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234">
        <property type="entry name" value="RIM BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234:SF22">
        <property type="entry name" value="RIMS-BINDING PROTEIN 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25523">
        <property type="entry name" value="Ig_RIMBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
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</entry>
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="1825" mass="194289" checksum="DD953F7F4C8F3D57" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZP46</accession>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            </authorList>
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    <sequence length="193" mass="22156" checksum="FEB342ECDD1F909E" modified="2020-06-17" version="1">IIPVCVLVLFEHFEHNKFNAYVTLKVQNVKSTTITVRGDQPCWEQDFMFEINHLESGLVVELWNKGLIWDTMIGTALIPLDSIRQSDEEGPGQWTSLDSEVLMREDEICGTSNPTPHQCTAWRYIPEDEAETLNFWPVAVDDRDSDYRSETGNRPPRFHNTPQTNSSVHQYPIGRRGSTRPCLDSLILYKAMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY12</accession>
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        <recommendedName>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <name>heme</name>
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        <text evidence="1">Belongs to the cytochrome P450 family.</text>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYP46A1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
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        <property type="entry name" value="Cyt_P450_sf"/>
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    <dbReference type="PANTHER" id="PTHR24293:SF0">
        <property type="entry name" value="CYP46A1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24293">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 46 SUBFAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="3">
        <location>
            <position position="330"/>
        </location>
        <ligand>
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        </ligand>
        <ligandPart>
            <name>Fe</name>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="44138" checksum="AFBBBE988F6ABFFF" modified="2020-06-17" version="1">LMSPKYPKNKNIYKRLSNLFGQRLGVMILCKTILISRYLRGLMGTFNERAEKLMEKLTEEADKKTEELSMLQLFNGVTLDVIAKEWSTMSGTDSFLYGFNAGVKSVELLIILLIFQSENLSLSNIQLNPKNRPFINEVREACRLLRATGAQWIHDRKTAIKNGVDVPKDVLIQILKTAGQDEELMVDNFVTFFIAGHETAANQLAFCVMELGRHPEIMEKVKKEVDDVIGMKMDIRYEDLGQLTYLSQVLKETLRLYSTVPGTSRDILEDMVIDGIHIPGGCVALFSSYVTGRMEKFFKDPLTFDPDRFHPDAPKPYYCYYPFALGSRACLGKNFAQMETKVVMAKLLQRFNFTFIPRQTFDLLDTETLRPKSGVLCSIRHRKHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZYF1</accession>
    <name>A0A672ZYF1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxiredoxin-6</fullName>
            <ecNumber evidence="12">1.11.1.27</ecNumber>
            <ecNumber evidence="13">2.3.1.23</ecNumber>
            <ecNumber evidence="2">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">1-Cys peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Acidic calcium-independent phospholipase A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glutathione-dependent peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Lysophosphatidylcholine acyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Non-selenium glutathione peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">prdx6</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:35983"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:35984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12937"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="2.3.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.27" evidence="12"/>
    <dbReference type="EC" id="2.3.1.23" evidence="13"/>
    <dbReference type="EC" id="3.1.1.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZYF1"/>
    <dbReference type="Ensembl" id="ENSSORT00005022918.1">
        <property type="protein sequence ID" value="ENSSORP00005022260.1"/>
        <property type="gene ID" value="ENSSORG00005010847.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZYF1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000001">
        <property type="entry name" value="1-Cys peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF4">
        <property type="entry name" value="PEROXIREDOXIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 21" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7 21" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="23">
        <location>
            <begin position="11"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="22">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="25403" checksum="81ED6BAB398BEF01" modified="2020-06-17" version="1">IGQEEPTMPGLLLGDEFPNFEADTTIGRIKFHDFLGNSWGILFSHPRDFTPVCTTELACAAKISDEFKKRGVKMIALSIDSVEDHRNWSKDVMAYNSAAETPLPFPIIADHKRELSVQLGMLDPDERDKDGMPLTARCVFVIGPDKKLKLSILYPATTGRNFDELLRVIDSLQLTAQKKVATPVDWKPGDKVMVIPSLSDAEAKSLFPNGVTTQEVPSGKKYLRYTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0J6</accession>
    <name>A0A673A0J6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibroblast growth factor</fullName>
            <shortName evidence="2">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115433343</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A0J6"/>
    <dbReference type="FunCoup" id="A0A673A0J6">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023656.1">
        <property type="protein sequence ID" value="ENSSORP00005022985.1"/>
        <property type="gene ID" value="ENSSORG00005011134.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A0J6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000001">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="214"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="248" mass="27512" checksum="3B1FC5D2EC75A018" modified="2020-06-17" version="1">MSRAAAIASSLIRQKRQAREREKANACRGSGSPSNSKGANDKPSKLNVFSRVKLFGSRKKRKRRRPPEPQLKGIVTRLSSRQGFQLQMQPDGTIDGTKDEDTVFNLIPVGLRVVAIQGVQTKLYLAMNNEGFLYTSEHFTPECKFKESVFENYYVTYSSMLYRQQASGRAWYLGLNKEGAIMKGNHVKKNKAAAHFIPKPLKVAMYREPSLHDLTELSRSGSGTPTKSRSASALLNGGGKTPSNNDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1J2</accession>
    <name>A0A673A1J2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methenyltetrahydrofolate synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mthfsd</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="Methenyltetrahydrofolate synthase domain-containing protein"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
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    </dbReference>
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        <property type="entry name" value="FTHF_cligase"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017:SF0">
        <property type="entry name" value="METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="368"/>
            <end position="441"/>
        </location>
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            <end position="359"/>
        </location>
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            <begin position="260"/>
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            <begin position="292"/>
            <end position="310"/>
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    </feature>
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        <source>
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            <dbReference type="PROSITE" id="PS50102"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023099.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="480" mass="52368" checksum="787C77B6B26D7D88" modified="2020-06-17" version="1">MEPVIKINPGATKCDIRQKVWDYIEDKNLANFPRPVHNRIPNFKGAFTACAKVSELQAFTQTAEVKVDPDKPLEGARLAVLQAQKTLLVPTPRLRTGLFNKITPPPGATKEQLRICSSSQGVKDFSVPIGLDAKVKVDLVVVGSVAVSEKGFRIGKGEGYADMEYGMMAAMGAVSESTVVVTIVHDCQVVDIPETLIEKHDLTVDYILTPTRVIKTDCQLPKPQGIIWTKLDTEKLEKIPILKKLRALEEQAGKDVTLGAAPAPAEPGLQTGQPRRQTKQRPRQNTQQGGEGEYKRDTKPEKRAASDQKTRQRPPRVRKESRGDGKGGSEGEIVEKAKGKSGGSMKEKGVEEGGNEVTSQRKIPVTVTTVYLGGIPAGLRVSELKTALREREAAPLRLTWQGAQHRAFLDYSDPQAADQALKALQGLSLNGHDLQAELAKSQRGGKRTGVSNRRPRPSTAQKSKTAPPETKGDTHEKNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A260</accession>
    <name>A0A673A260_9TELE</name>
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        <submittedName>
            <fullName evidence="6">Phosphofurin acidic cluster sorting protein 2</fullName>
        </submittedName>
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        <name evidence="6" type="primary">pacs2</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the PACS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A260"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:protein localization to plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280:SF15">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN 2"/>
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    </dbReference>
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        <property type="entry name" value="Pacs-1"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF10254"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="878" mass="98400" checksum="00B9103E72FAC897" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A2U0</accession>
    <name>A0A673A2U0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Synaptonemal complex protein 3</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">sycp3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Sphaeramia</taxon>
        </lineage>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the XLR/SYCP3 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A673A2U0"/>
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        <property type="protein sequence ID" value="ENSSORP00005022977.1"/>
        <property type="gene ID" value="ENSSORG00005011116.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="XLR/SYCP3"/>
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        <property type="entry name" value="XLR/SYCP3/FAM9_dom"/>
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        <property type="entry name" value="XLR/SCP3/FAM9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="XLR_SYCP3_FAM9 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="86"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="266" mass="31143" checksum="E0C96862A94344D7" modified="2020-06-17" version="1">SMPPGRRPMKRKLPEEKSDKKENVFDFTQDQKELSGSEDEVGQGESVIMDKIAKKRPVADFEDEDGACPVGYELHHLLLLATDISKVMQTKKKRLESLTKNYMKGSQQKLEQLWSNYHTQRLVFNWHNHLELNTVQVNFVKPFIEFLSSLQNLFRQQQKVLQQARVVQNQKLKTVRELYEQFMKNIEDMEKSHSVFLQGAQQELKKEMATLQKKILMDTVSRLSSVVFTFYKLSQNVYCCIWSADVFLLEILAKVVFIHSSHLEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3A3</accession>
    <name>A0A673A3A3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CAAX prenyl protease 2</fullName>
            <ecNumber evidence="11">3.4.26.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Farnesylated proteins-converting enzyme 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115433444</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A673A3A3"/>
    <dbReference type="Ensembl" id="ENSSORT00005023780.1">
        <property type="protein sequence ID" value="ENSSORP00005023108.1"/>
        <property type="gene ID" value="ENSSORG00005011187.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A3A3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="14">
        <location>
            <begin position="158"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="319" mass="35397" checksum="F953411D6BE45FAE" modified="2020-06-17" version="1">MFVCASSHYSIKTLSSAMFEPEHAAVHQEDHAISRCWVGVLNCLLLSCLYVGSLYIWRSSLPRDHPSVIKRRCVSVLLVSALSPAVVKTWMHWSDVGASVWELMGIRLDGFVPAAVLPLLLTMVFYLGPLVHSVMENPKGFTGELQSASWRLCVEDAVWLRNQVVAPVTEELVFRGAMLPMLVPCTGPRAAVFLAPLFFGVAHFHHIIEQRRCENLSSLCCFLFIGMQFLYTTVFGAYTAFIFMRTGHVVGPILCHSFCNSQGLPDISSALKHPQRPALLFSYLMGLLLFLVLLFPLTDSFFYGVIPICSLAPPPVSAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A3B1</accession>
    <name>A0A673A3B1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycerophosphodiester phosphodiesterase domain-containing protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gdpd5a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A3B1"/>
    <dbReference type="Ensembl" id="ENSSORT00005023790.1">
        <property type="protein sequence ID" value="ENSSORP00005023118.1"/>
        <property type="gene ID" value="ENSSORG00005011195.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A3B1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344:SF49">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE DOMAIN-CONTAINING 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="9">
        <location>
            <begin position="228"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="583" mass="66971" checksum="84D65D71F54C2806" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4M5</accession>
    <name>A0A673A4M5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">plpp7</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030006543.1">
        <property type="nucleotide sequence ID" value="XM_030150683.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030006544.1">
        <property type="nucleotide sequence ID" value="XM_030150684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A4M5"/>
    <dbReference type="FunCoup" id="A0A673A4M5">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024947.1">
        <property type="protein sequence ID" value="ENSSORP00005024236.1"/>
        <property type="gene ID" value="ENSSORG00005011670.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430591"/>
    <dbReference type="InParanoid" id="A0A673A4M5"/>
    <dbReference type="OrthoDB" id="10266771at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03391">
        <property type="entry name" value="PAP2_containing_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF17">
        <property type="entry name" value="INACTIVE PHOSPHOLIPID PHOSPHATASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="3">
        <location>
            <begin position="153"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="289" mass="31782" checksum="0F0F56BDD684555E" modified="2020-06-17" version="1">MPSIHNVRSRTRERNSVLSRPEFMSLNHQPVRGGPPDSRGSAPKRPAQMKPQPSEEPADTPGKERREPGREPGRMPEEDCMQLNPSFKGIAINSLLAIDICLSKRMGVCAYTSSSWGGCRSMVALLALTGHGITWIIGTILCLTRSNTLAGQEVLVNLLMALILDVMTVAGVQRLVKRRGPWEMTPGFLDCVAMDVYSFPAAHASRAAMVSKFLLSHLVLAVPLRILLVLWAFLVGMSRVVLGKHHLTDMVCGFALGMLHFSLMETVWLSSSTCQKLISISTLSWSPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACH1</accession>
    <name>A0A673ACH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115433013</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030010044.1">
        <property type="nucleotide sequence ID" value="XM_030154184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ACH1"/>
    <dbReference type="SMR" id="A0A673ACH1"/>
    <dbReference type="FunCoup" id="A0A673ACH1">
        <property type="interactions" value="1250"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027229.1">
        <property type="protein sequence ID" value="ENSSORP00005026453.1"/>
        <property type="gene ID" value="ENSSORG00005012671.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433013"/>
    <dbReference type="CTD" id="449794"/>
    <dbReference type="InParanoid" id="A0A673ACH1"/>
    <dbReference type="OrthoDB" id="10253031at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000004">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="14">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="128" mass="13731" checksum="8FF52164A8052D6D" modified="2020-06-17" version="1">MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTEKPAKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADD7</accession>
    <name>A0A673ADD7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL43</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L43, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mrpl43</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL43 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029977552.1">
        <property type="nucleotide sequence ID" value="XM_030121692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ADD7"/>
    <dbReference type="FunCoup" id="A0A673ADD7">
        <property type="interactions" value="1388"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027556.1">
        <property type="protein sequence ID" value="ENSSORP00005026773.1"/>
        <property type="gene ID" value="ENSSORG00005012828.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410194"/>
    <dbReference type="CTD" id="84545"/>
    <dbReference type="InParanoid" id="A0A673ADD7"/>
    <dbReference type="OrthoDB" id="88at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000078">
        <property type="entry name" value="39S ribosomal protein L43, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039927">
        <property type="entry name" value="Ribosomal_mL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="10">
        <location>
            <begin position="35"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="147" mass="16443" checksum="03D948E2BC0E4AA5" modified="2020-06-17" version="1">MTSRGTPSRFLKSVLQNGVGRYVCQLKRISIIFSKKAQSSLGVREFIEEGVVDYAKNNPGTVVYVSPQSSRIPKIVAEYLNGNVREEIITSKTAPQISELLTKLTNQSGLDIIRIRKRVHTDSPSIQGQWHPFTNRPPSIGPIRPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ADV1</accession>
    <name>A0A673ADV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ADV1"/>
    <dbReference type="Ensembl" id="ENSSORT00005028196.1">
        <property type="protein sequence ID" value="ENSSORP00005027409.1"/>
        <property type="gene ID" value="ENSSORG00005013082.1"/>
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        <property type="component" value="Chromosome 18"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
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        <property type="entry name" value="Tc1-like_DDE_dom"/>
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        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
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        <property type="entry name" value="TRANSPOSABLE ELEMENT TC1 TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="68"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="117"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="36502" checksum="132E04FA19D51AB8" modified="2020-06-17" version="1">VRAHVAQRLGCGRRVIDRLAARYRETGQVVDRPRSGHPRVTTQRQDAYIVLSARHRNLRSASRLRDDLQTTRNRLHARGIHARRPVVRPVLTRQHVANRLAWCRTHLHWTMANWRMMVFSDESRFMVTPTDGRARVWWERGQRYVANNVLHRDRWGAGSVMVWGAIWWTGRSNLIIVHGNLNGHGYIENILRPEVARIMRQLGPKAVFQDDNARPHRSSAVDQFFEDEGINHMDWPARSPDLNPIEHLWDMLDRQVRSWNHQLRSVQELTQWIQEEWRAIPHQQIQTLIGSMRRRCTAVIDARGRHSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AFA8</accession>
    <name>A0A673AFA8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Microfibril-associated glycoprotein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115410734</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029978373.1">
        <property type="nucleotide sequence ID" value="XM_030122513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AFA8"/>
    <dbReference type="Ensembl" id="ENSSORT00005028736.1">
        <property type="protein sequence ID" value="ENSSORP00005027936.1"/>
        <property type="gene ID" value="ENSSORG00005013375.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410734"/>
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    <dbReference type="OrthoDB" id="7735550at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048251">
        <property type="term" value="P:elastic fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF225">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025465147" evidence="2">
        <location>
            <begin position="25"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="24"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="242" mass="27625" checksum="C9FB47602FCD021F" modified="2020-06-17" version="1">MKMVQMVQMVLVLVVLVCPVWTSSYSLDGPVDCSDIYLTSNQTGVYTVHPLDGRSIQVYCDMDTEGGGWTVFQRRMDGSVNFYRPWDQYKKGFGDLNGEYWLGLDNIHYLTRRRPYELLVLMSDFDGNNVSARYSTFFVGGEHRGYELHVTGFTDGGAGDSLSYHSGMKFTTWDKDQDKYGSNCARVYLGAFWHNGCHYANPNGVYLWGADGAQTSTGVLWYTWKGYYYSVQTFIMMIRPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AFP9</accession>
    <name>A0A673AFP9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Potassium voltage-gated channel subfamily G member 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">kcng2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AFP9"/>
    <dbReference type="FunCoup" id="A0A673AFP9">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029307.1">
        <property type="protein sequence ID" value="ENSSORP00005028495.1"/>
        <property type="gene ID" value="ENSSORG00005013616.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AFP9"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000024">
        <property type="entry name" value="Potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000019">
        <property type="entry name" value="Potassium voltage-gated channel, subfamily G, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF90">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY G MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="16">
        <location>
            <begin position="62"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="228"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="177"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="475"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="534" mass="60435" checksum="0C8A94DDF5426048" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGG1</accession>
    <name>A0A673AGG1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA-splicing endonuclease subunit Sen15-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115437069</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN15 family.</text>
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    <dbReference type="RefSeq" id="XP_030016014.1">
        <property type="nucleotide sequence ID" value="XM_030160154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AGG1"/>
    <dbReference type="FunCoup" id="A0A673AGG1">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028599.1">
        <property type="protein sequence ID" value="ENSSORP00005027803.1"/>
        <property type="gene ID" value="ENSSORG00005013304.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437069"/>
    <dbReference type="InParanoid" id="A0A673AGG1"/>
    <dbReference type="OrthoDB" id="10002170at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018593">
        <property type="entry name" value="tRNA-endonuc_su_Sen15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28582">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28582:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF09631">
        <property type="entry name" value="Sen15"/>
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    </dbReference>
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        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="42"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="151" mass="17227" checksum="D9D75AFD4A994302" modified="2020-06-17" version="1">MSESGDESEKKQPTNWIQQHPVYQGMKSLEVGDSAQIHAAFLVYMDLSEARHWNEVSYMKSPELEVVLLEGREIEGGPVQTVLPLPVHQNVNHRSLRRVLDRGFPLLLCAVGSDSTLVYQRMTDGLVTPDPPVGPFQDVDRRHQRKRRQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGS7</accession>
    <name>A0A673AGS7_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">btbd2</name>
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    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030015815.1">
        <property type="nucleotide sequence ID" value="XM_030159955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AGS7"/>
    <dbReference type="Ensembl" id="ENSSORT00005029718.1">
        <property type="protein sequence ID" value="ENSSORP00005028900.1"/>
        <property type="gene ID" value="ENSSORG00005013759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436935"/>
    <dbReference type="InParanoid" id="A0A673AGS7"/>
    <dbReference type="OrthoDB" id="636773at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18281">
        <property type="entry name" value="BTB_POZ_BTBD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000037">
        <property type="entry name" value="BTB (POZ) domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000004">
        <property type="entry name" value="BTB/POZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.820:FF:000004">
        <property type="entry name" value="BTB/POZ domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.820">
        <property type="entry name" value="PHR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012983">
        <property type="entry name" value="PHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038648">
        <property type="entry name" value="PHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774:SF3">
        <property type="entry name" value="BTB (POZ) DOMAIN-CONTAINING 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08005">
        <property type="entry name" value="PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="167"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="575" mass="61586" checksum="575FA82AB949C0A7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AH28</accession>
    <name>A0A673AH28_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Calcitonin gene-related peptide-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115421608</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the calcitonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993427.1">
        <property type="nucleotide sequence ID" value="XM_030137567.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029993430.1">
        <property type="nucleotide sequence ID" value="XM_030137570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AH28"/>
    <dbReference type="FunCoup" id="A0A673AH28">
        <property type="interactions" value="972"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028446.1">
        <property type="protein sequence ID" value="ENSSORP00005027652.1"/>
        <property type="gene ID" value="ENSSORG00005013211.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421608"/>
    <dbReference type="InParanoid" id="A0A673AH28"/>
    <dbReference type="OrthoDB" id="9929923at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031716">
        <property type="term" value="F:calcitonin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021118">
        <property type="entry name" value="Calcitonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021117">
        <property type="entry name" value="Calcitonin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018360">
        <property type="entry name" value="Calcitonin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001693">
        <property type="entry name" value="Calcitonin_peptide-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10505:SF16">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10505">
        <property type="entry name" value="CALCITONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00270">
        <property type="entry name" value="CALCITONINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00113">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00258">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0027">Amidation</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025446321" evidence="12">
        <location>
            <begin position="26"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Calcitonin peptide-like" evidence="13">
        <location>
            <begin position="81"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="83"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR621116-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="135" mass="14874" checksum="A8FDCC459CF61403" modified="2020-06-17" version="1">MVMLKISAFLVAYALVICQMYSSQAAPARPGLESMSDRVTLTDYEARRLLNAIVKEFVQMTAEELEQQATDGNSMDRPIAKRCSNLSTCVLGKLSQELHKLQTFPRTNVGSGTPGKKRSPDTDMYAGYGDAFDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIV7</accession>
    <name>A0A673AIV7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AIV7"/>
    <dbReference type="Ensembl" id="ENSSORT00005029416.1">
        <property type="protein sequence ID" value="ENSSORP00005028603.1"/>
        <property type="gene ID" value="ENSSORG00005013685.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AIV7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002019">
        <property type="entry name" value="zinc finger protein 263-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF445">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF28.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="65"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="427" mass="46781" checksum="E419ED3C5BB5492C" modified="2020-06-17" version="1">MLSGFMCIFHMPLLTSDLGLNNILFSFGCLSYNPLCLSLQYPQMEGYQPASLQLIKEALKMDPPNQNLHSCSRAQSEGELSDQPPTLHPGEKEEEEDWEVGSGERSEGGMTVEELRGLESALRAERGREQAAMKSSRPVSPDSVAAQGSEIGLAPKYIGLDGMEQDGEHEPEPPTLQREDQIGQARVKDDPMTGVVGGVEIRAGWLKRMREGDSGGVVAAEDMSEQGESEHLPHLSTPGSVGESGGEEEGGGDLLHFCPQCGGGFTSEAELDEHPCPLGGAHLQSSGAEHSLFPCASCGNTFSHAWALKNHECACAAERPHCCEICGKRFTHSRSLERHHLVHTGERPHQCPQCGRSFSRLGNLERHQRIHTGERPYGCEACGKRFSRVEYLKRHQLIHNSEKAALQCSNCSRSFSDVEQLKNHQCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJ89</accession>
    <name>A0A673AJ89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cdc34b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673AJ89"/>
    <dbReference type="Ensembl" id="ENSSORT00005029181.1">
        <property type="protein sequence ID" value="ENSSORP00005028372.1"/>
        <property type="gene ID" value="ENSSORG00005013577.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJ89"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23803">
        <property type="entry name" value="UBCc_UBE2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000009">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="12">
        <location>
            <begin position="11"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="238" mass="27129" checksum="AC80F0B00DB40C61" modified="2020-06-17" version="1">MAQHDPSHVASSQKALMLEMKSLQEEPVEGFKITLVDEADLYNWEVAIFGPPNTHYEGGYFKARIKFPIDYPYSPPAFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWRDSKGKDREYVEIIRKQVLATKAEAERDGVKVPTTLAEYCVRTRAPPPDEGSDLFYDYYYDDEDVEGGDGDCCYDEDDSGNEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJA2</accession>
    <name>A0A673AJA2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="518" mass="59777" checksum="B27F144A83AB908E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKA6</accession>
    <name>A0A673AKA6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cirbpa</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AKA6"/>
    <dbReference type="Ensembl" id="ENSSORT00005029913.1">
        <property type="protein sequence ID" value="ENSSORP00005029093.1"/>
        <property type="gene ID" value="ENSSORG00005013886.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Cold inducible RNA binding protein a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="5"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="66"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="192" mass="20219" checksum="45DD57DDDEFE352F" modified="2020-06-17" version="1">MSDEGKLFIGGLSFDTTEESLAAAFGKYGTIEKVDVIKDRETGRSRGFGFVKYDNPEDARDAMDAMNGKSLDGRHIRVDEAGKGGRPRGGGGFGSGQRGSRFGTSRGRGGGGRGYSRGGGGYNGDRGYGDRSYGERSFSSGDRSFGGGQYRSGGGGGGYSSGGGYRENRSQGGYSDRSSSYREGYDNYATHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ALG2</accession>
    <name>A0A673ALG2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">vasnb</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ALG2"/>
    <dbReference type="FunCoup" id="A0A673ALG2">
        <property type="interactions" value="879"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031375.1">
        <property type="protein sequence ID" value="ENSSORP00005030520.1"/>
        <property type="gene ID" value="ENSSORG00005014559.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ALG2"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000211">
        <property type="entry name" value="vasorin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF160">
        <property type="entry name" value="VASORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025663367" description="EGF-like domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="584"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="416"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="368"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="684" mass="75507" checksum="E0064478961DDEE7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AX35</accession>
    <name>A0A673AX35_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115412944</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AX35"/>
    <dbReference type="Ensembl" id="ENSSORT00005033779.1">
        <property type="protein sequence ID" value="ENSSORP00005032878.1"/>
        <property type="gene ID" value="ENSSORG00005015596.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AX35"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03716">
        <property type="entry name" value="SOCS_ASB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000016">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF17">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="7">
        <location>
            <begin position="233"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="290" mass="31390" checksum="0F6D031A42727CE0" modified="2020-06-17" version="1">MYTSCHADVVLNVFCRCFLRIDTESDWSQIHDAAFNGRVLALQNLITQGACVNLNTLDRVSPLHAACVQGHTACAKVLVENGAHVNCSTVDGQTPLSEACARGHVTCVTLLLQHGAAPQGTSHSNSPLHSAAAKGHAECIESLVQHGADVDQYMERSGSPLNMACSNQQLSTVRKLLQLGASVNSVVHGDSPLHTAARLSSPEMVSILLDHGADRHLRNLEGKHPLDLAPPNSLTEKLLKQAETSSLMQLCRLFIRRIAGKHRLGEIHDLRLPTELKRYLLHQSDPGGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AXN4</accession>
    <name>A0A673AXN4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115415195</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A673AXN4"/>
    <dbReference type="Ensembl" id="ENSSORT00005035329.1">
        <property type="protein sequence ID" value="ENSSORP00005034410.1"/>
        <property type="gene ID" value="ENSSORG00005013676.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AXN4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11729">
        <property type="entry name" value="ADDz_Dnmt3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000006">
        <property type="entry name" value="DNA (Cytosine-5)-methyltransferase 3B isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000011">
        <property type="entry name" value="DNA methyltransferase 3 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.50">
        <property type="entry name" value="PWWP, helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025766">
        <property type="entry name" value="ADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044108">
        <property type="entry name" value="ADD_DNMT3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018117">
        <property type="entry name" value="C5_DNA_meth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054724">
        <property type="entry name" value="DNM3A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040552">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049554">
        <property type="entry name" value="DNMT3_ADD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068:SF10">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068">
        <property type="entry name" value="DNA CYTOSINE-5- -METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17980">
        <property type="entry name" value="ADD_DNMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22855">
        <property type="entry name" value="DNM3A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21255">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51533">
        <property type="entry name" value="ADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00094">
        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="198"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="383"/>
            <end position="515"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="144"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="787" mass="89484" checksum="B600F98B85F5AC68" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B007</accession>
    <name>A0A673B007_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Regulator of cell cycle</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rgcc</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029980948.1">
        <property type="nucleotide sequence ID" value="XM_030125088.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="REGULATOR OF CELL CYCLE RGCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15151">
        <property type="entry name" value="RGCC"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="49"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14177" checksum="4328561E05E85543" modified="2020-06-17" version="1">MKSPKLKAQAKFVSEEDLNDVLCEFDAVIEDFTSPVEKRHFRYDEHLKTMKRRSSASVSDSGISDSESAESLNRNSFSFSDERLNSPTVLSPNPTSPSLMSPKPKLGDTKELEDFIADLDRTLETM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2P3</accession>
    <name>A0A673B2P3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tmem18</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029985001.1">
        <property type="nucleotide sequence ID" value="XM_030129141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B2P3"/>
    <dbReference type="FunCoup" id="A0A673B2P3">
        <property type="interactions" value="558"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005035860.1">
        <property type="protein sequence ID" value="ENSSORP00005034932.1"/>
        <property type="gene ID" value="ENSSORG00005016458.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415545"/>
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    <dbReference type="InParanoid" id="A0A673B2P3"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
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        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026721">
        <property type="entry name" value="TMEM18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22593">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22593:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14770">
        <property type="entry name" value="TMEM18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="152" mass="17845" checksum="3C811E5AD4F3C026" modified="2020-06-17" version="1">MTDQKAENISSIPIDGFSNLRITSIWTFLMSVQWSEPWLIGLVLFHIVCLCVTVVTCRYYRAQICHFLLMVVLVYGAEYLNELAAMNWRSFSNFQYFDSKGMFISLVYSIPLLLNTVIIVMVWVHRTFSTMTELKTLQLKRKARRENREKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3X3</accession>
    <name>A0A673B3X3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Rho-related GTP-binding protein RhoU-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115418445</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029988801.1">
        <property type="nucleotide sequence ID" value="XM_030132941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B3X3"/>
    <dbReference type="Ensembl" id="ENSSORT00005036322.1">
        <property type="protein sequence ID" value="ENSSORP00005035372.1"/>
        <property type="gene ID" value="ENSSORG00005016699.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115418445"/>
    <dbReference type="InParanoid" id="A0A673B3X3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04130">
        <property type="entry name" value="Wrch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000561">
        <property type="entry name" value="rho-related GTP-binding protein RhoV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="272" mass="30422" checksum="C684BA10A4A197D4" modified="2020-06-17" version="1">MLPQDVGKQKSRRVSDPVFAPPVPPVPPRLKNWDFPKSVKRRRSGATPERKVNCVLVGDGAVGKTSLIVSYTTNGYPTEYVPTAFDNFTAMVVVDGKPVRLQLCDMAGQDELERLRPLCYKNADVFLLCYSVVRPCSFRNLTDRWVPEIRQHCPGAPVVLVGTQLDLREDVQVLIHLAHNHQQPVGTEEGQRLAHELGAVSFSECSALTQKNLKDAFDAAILASIQHMDSSSSIQQERLTLRRKTPDKIKSLSETWWKKINCLMGEQSCELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5Q8</accession>
    <name>A0A673B5Q8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L1 transposable element RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B5Q8"/>
    <dbReference type="Ensembl" id="ENSSORT00005038123.1">
        <property type="protein sequence ID" value="ENSSORP00005037150.1"/>
        <property type="gene ID" value="ENSSORG00005017455.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5Q8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39470" checksum="FFA5598DF7834DF7" modified="2020-06-17" version="1">MDNYLERLTKAFGFFIYFSCNSIVKAELGNAWDRVNSQLLIFVPLVVIKTFSEVVEYVCLACVMAEISLQSIWEAIHQMKEQVSAQVHAHLDLKIGTVQAGLMDIQQTLNSVVGQSTELQQRVSTNEDNVAKLLGRVEKDNTYLKEKVEDLENWSTRYKPSFINVPEESEGGDIVAFMSQQLIPKLLGRESFPKAPVVERAHRSPTFNTSVRLGASRPRPILVKFLNFQDKVKIMRLAKEKGDLVFEGNTIHIYPDFSPGIVKKRREYDAVKRKLHATDMEYSLLYPDMLRVMVKGKPKLFRSSKEAKDFFQDLSPESTTVANWREEATYSSEKLQRFFFPLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B8J2</accession>
    <name>A0A673B8J2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">rab40b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673B8J2"/>
    <dbReference type="Ensembl" id="ENSSORT00005039161.1">
        <property type="protein sequence ID" value="ENSSORP00005038170.1"/>
        <property type="gene ID" value="ENSSORG00005017868.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B8J2"/>
    <dbReference type="OrthoDB" id="6339763at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04121">
        <property type="entry name" value="Rab40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000371">
        <property type="entry name" value="RAB40C, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SOCS box" evidence="17">
        <location>
            <begin position="181"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="282" mass="31537" checksum="4C3399CA040707FE" modified="2020-06-17" version="1">MSHRSPAKAYDFLLKFLLVGDSDVGKGEILSSLEDGASETPYGHNMGIDYKTTTILLDGRRVKLQLWDTSGQGRFCTIFRSYSRGAQGVILVYDITNRWSFDGIDRWIKEIDEHAPGVPKILVGNRLHLAYKRQVTTEQAQVYAERLGVTFFEVSPLCNFNITESFTELARIVLMRHGMERLWRPSRVLSLQDLCCRSIVSCTPVHLVDKLPLPLALKSHLKSFSVANGLNARMMHGRSYSVTAHAATKRTGGTLLKKPKLIRAPPLSPSAQSCTRNSCKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B918</accession>
    <name>A0A673B918_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3'-5' exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">exd3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030005498.1">
        <property type="nucleotide sequence ID" value="XM_030149638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B918"/>
    <dbReference type="Ensembl" id="ENSSORT00005038067.1">
        <property type="protein sequence ID" value="ENSSORP00005037097.1"/>
        <property type="gene ID" value="ENSSORG00005017420.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429856"/>
    <dbReference type="CTD" id="54932"/>
    <dbReference type="InParanoid" id="A0A673B918"/>
    <dbReference type="OrthoDB" id="18193at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052408">
        <property type="entry name" value="Exonuclease_MUT-7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765">
        <property type="entry name" value="3'-5' EXONUCLEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765:SF2">
        <property type="entry name" value="EXONUCLEASE MUT-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="2">
        <location>
            <begin position="374"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="891" mass="100861" checksum="C5541A36B821A81B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9R9</accession>
    <name>A0A673B9R9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein phosphatase 1 regulatory subunit 35-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115416213</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP1R35 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029985814.1">
        <property type="nucleotide sequence ID" value="XM_030129954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B9R9"/>
    <dbReference type="FunCoup" id="A0A673B9R9">
        <property type="interactions" value="727"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038326.1">
        <property type="protein sequence ID" value="ENSSORP00005037352.1"/>
        <property type="gene ID" value="ENSSORG00005017531.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416213"/>
    <dbReference type="InParanoid" id="A0A673B9R9"/>
    <dbReference type="OrthoDB" id="8942190at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903724">
        <property type="term" value="P:positive regulation of centriole elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045724">
        <property type="term" value="P:positive regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033590">
        <property type="entry name" value="PPP1R35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029135">
        <property type="entry name" value="PPP1R35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28625">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28625:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15503">
        <property type="entry name" value="PPP1R35_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 35 C-terminal" evidence="6">
        <location>
            <begin position="156"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="301" mass="33221" checksum="81269888DF177344" modified="2020-06-17" version="1">MWERNRSGSEPIQSRMSGPSPLLSPPPSSHPPPQPHPVPFDSYSCTGCPELDLSVTLSPAPRATPAVLKPHPQPATLHLKPCPRGQRKMKNPEVCFQPVVVTVTPQPHLTHPQQPIRSQRSSIVHLHGNHSGASTRWEEPPAPQPSDGPVGLAEAELHTTLALKVELQSLQGVEFNSQKAIQETLCRSTWTRSLINSRVTEGLNVGRSQLLFSSLVSVDVPADQLIDRALQDRLPLAPPPRCRSIRKPTDSPALFPNVSDLQRQKPLPPEEQGPARLRPLPFSSCSTFDLYSRQRRWEATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B9U0</accession>
    <name>A0A673B9U0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Regulator of G protein signaling 9a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rgs9a</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B9U0"/>
    <dbReference type="FunCoup" id="A0A673B9U0">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039603.1">
        <property type="protein sequence ID" value="ENSSORP00005038610.1"/>
        <property type="gene ID" value="ENSSORG00005018037.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B9U0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04450">
        <property type="entry name" value="DEP_RGS7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047016">
        <property type="entry name" value="RGS6/7/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047017">
        <property type="entry name" value="RGS6/7/9/11_DHEX_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040759">
        <property type="entry name" value="RGS_DHEX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746">
        <property type="entry name" value="LP21163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746:SF8">
        <property type="entry name" value="REGULATOR OF G PROTEIN-SIGNALING 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18148">
        <property type="entry name" value="RGS_DHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="275"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="453" mass="52759" checksum="DA58AF99A5DA4A32" modified="2020-06-17" version="1">MQDSKSGIKGSDQKLNVTTIPYVITGKDVIAWIANKMKTTVEGLAAQVFGNMLVAYGYIYPLQNHKKLVMCNDNTLYRFQTPYFWPTQKWVAEDSDYAIYLAKRNIRKKGMLEPYEQAHYNHLHKWLNHKWDFIVMQATEQYKAGKERKKPDRVVFDCQERAYWMINRPPRRTHSAMDCGPDRLIDPSVEEKITFDQYRRMNIFYQQAIMRSKVKSSVSLGALVKYVTTYKNHDAFLAPCLPSNPWQTDSDAYWNLNMRRVDVPTKMRVERWSFSLFELLTDLRGRDDFKIFLKKEFSGENLAFWEAAEELKWGTASSMKTKAETIFKTFLAPGAPRWINIDGRTMGLTVKGLEHPHRYVLEAAQTHVFLLMKKDTFFRYLKSPVYKDIQKKALNPEPHNFSPAQLEQNAQSRGPGIHPIILWQQEEEEKAKAAAASAPVDVKAMMSKIDRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB39</accession>
    <name>A0A673BB39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myotubularin-related protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Inactive phosphatidylinositol 3-phosphatase 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mtmr9</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029984710.1">
        <property type="nucleotide sequence ID" value="XM_030128850.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029984711.1">
        <property type="nucleotide sequence ID" value="XM_030128851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BB39"/>
    <dbReference type="FunCoup" id="A0A673BB39">
        <property type="interactions" value="971"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038655.1">
        <property type="protein sequence ID" value="ENSSORP00005037673.1"/>
        <property type="gene ID" value="ENSSORG00005017666.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415324"/>
    <dbReference type="CTD" id="66036"/>
    <dbReference type="InParanoid" id="A0A673BB39"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13211">
        <property type="entry name" value="PH-GRAM_MTMR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14536">
        <property type="entry name" value="PTP-MTMR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000240">
        <property type="entry name" value="Myotubularin-related protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048994">
        <property type="entry name" value="PH-GRAM_MTMR6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF56">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21098">
        <property type="entry name" value="PH-GRAM_MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="14">
        <location>
            <begin position="123"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="549" mass="62677" checksum="ED993D87BD9052C7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BDK4</accession>
    <name>A0A673BDK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115432583</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030009355.1">
        <property type="nucleotide sequence ID" value="XM_030153495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BDK4"/>
    <dbReference type="FunCoup" id="A0A673BDK4">
        <property type="interactions" value="186"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039470.1">
        <property type="protein sequence ID" value="ENSSORP00005038478.1"/>
        <property type="gene ID" value="ENSSORG00005017968.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432583"/>
    <dbReference type="InParanoid" id="A0A673BDK4"/>
    <dbReference type="OrthoDB" id="9975554at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF359">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="63"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
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        <location>
            <begin position="144"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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        </source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="335" mass="38408" checksum="F177A62CF66D1580" modified="2020-06-17" version="1">MIQADDNYTQVSAFVIVGFPSLQPEYFDIVGWFFFFLYVTIVLGNLLLVVVFALEHSIQKPMYIIMLSLALSDIGFPTVALPKLIARYWWNDGRLSFQTCLFQRHMIHYFGSLNSLILLTMALDRYFAICFPLRYPTVMTNQTMTGLTVLTWVVAHIFPSVSSYHIPTMLFCGLNKIMNVFCDTRSVVALVCGDISRKHMQSFATALFILYAPLSLIVISYIALIVTVLHVDSGKGLVKTFSTFATQGCIISIYYIPRFFVYAAPFFPSLTMTPDKRIAATLFYSLFPPLINPFIYCLRTKEIKQILRRWIQKRKSVFPKSIKAFPKSMVKIEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFU7</accession>
    <name>A0A673BFU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Calreticulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115437793</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasmic vesicle</location>
            <location evidence="15">Secretory vesicle</location>
            <location evidence="15">Cortical granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16 19">Belongs to the calreticulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016998.1">
        <property type="nucleotide sequence ID" value="XM_030161138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BFU7"/>
    <dbReference type="FunCoup" id="A0A673BFU7">
        <property type="interactions" value="2287"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041206.1">
        <property type="protein sequence ID" value="ENSSORP00005040169.1"/>
        <property type="gene ID" value="ENSSORG00005018734.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437793"/>
    <dbReference type="InParanoid" id="A0A673BFU7"/>
    <dbReference type="OrthoDB" id="1938156at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060473">
        <property type="term" value="C:cortical granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calreticulin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009169">
        <property type="entry name" value="Calreticulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF42">
        <property type="entry name" value="CALRETICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002356">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00805">
        <property type="entry name" value="CALRETICULIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 16 19" id="KW-0143">Chaperone</keyword>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 16 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025710155" description="Calreticulin" evidence="19">
        <location>
            <begin position="19"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="195"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="337"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="206"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="350"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="376"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
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        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR002356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="419" mass="48713" checksum="58C5C2D0FC2B750A" modified="2020-06-17" version="1">MKLLGVLAVVLALHSAHAKVYFREEFLDGDEWRSRWVDSKHKSDYGEWKLTSGGFYGDAEKDKGLQTSQDARFYATSARFEPFSNEGKSLVIQFTVKHEQKIDCGGGYVKVFPADLDQTDMHGESAYYIMFGPDICGYSTKKVHVIFNYKGKNHLIKKEIKCKDDELTHLYTLVLNPDQTYEVKIDNEKVESGSLEDDWDFLPPKKIKDPEAKKPEDWDDRAKIDDAEDTKPVDWDKPENIPDPDAKKPDDWDEDMDGEWEPPMIPNPEYKGEWKPKQIDNPNYKGQWVHPEIDNPEYSPDSDIYTFDKIAVLGLDLWQVKSGTIFDNFLITDDVKEAEDIGNETWGVTKEPERKMKQEQDDMKRKEEEERNKEQDTEDTEDDEEEDEEDDEDVEDTKDDTEEGLSEMDKKEVKSKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BJ74</accession>
    <name>A0A673BJ74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">aatkb</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="Ensembl" id="ENSSORT00005043722.1">
        <property type="protein sequence ID" value="ENSSORP00005042636.1"/>
        <property type="gene ID" value="ENSSORG00005019746.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BJ74"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000347">
        <property type="entry name" value="Apoptosis associated tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000275">
        <property type="entry name" value="Apoptosis associated tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24417">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE LMTK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24417:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE LMTK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="126"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1030"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1209"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1357"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1069"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1097"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1152"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1238"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1260"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1406"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1465" mass="163121" checksum="831180D28EE92BC4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLB4</accession>
    <name>A0A673BLB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BLB4"/>
    <dbReference type="Ensembl" id="ENSSORT00005042124.1">
        <property type="protein sequence ID" value="ENSSORP00005041068.1"/>
        <property type="gene ID" value="ENSSORG00005019132.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLB4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Zn peptidases"/>
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    </dbReference>
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        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
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        <property type="entry name" value="CARBOXYPEPTIDASE X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
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    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="9">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
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        <location>
            <position position="95"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="182" mass="21176" checksum="CE0C392C34A969B5" modified="2020-06-17" version="1">MQDIPFVLSANLHGGEVVVTYPFDCTRDWAPQEDTPTADNAFFRWLATVYASTNLMMDFQSHNNIINGGAWHTVPGVSMNDFSYLHTNCFEVTVELSCDKFPHASELPFEWENNKESLLVYMEQVHRGIKGVVRDKLTRKGIPDAIIKVEDHDHDIRSGRGWRRRYHDDRNRQWVHIQVNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BPD3</accession>
    <name>A0A673BPD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2</fullName>
            <ecNumber evidence="3">2.7.1.105</ecNumber>
            <ecNumber evidence="4">3.1.3.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">6PF-2-K/Fru-2,6-P2ase heart-type isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pfkfb2b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Synthesis and degradation of fructose 2,6-bisphosphate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Forms a heterodimer with PFKFB3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
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    <dbReference type="EC" id="3.1.3.46" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673BPD3"/>
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        <property type="protein sequence ID" value="ENSSORP00005042457.1"/>
        <property type="gene ID" value="ENSSORG00005016833.1"/>
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    <dbReference type="InParanoid" id="A0A673BPD3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:fructose metabolic process"/>
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        <property type="entry name" value="HP_PGM_like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
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        <property type="entry name" value="6Pfruct_kin"/>
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        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
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        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
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        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF48">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
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        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="19">
        <location>
            <begin position="27"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="16">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="16">
        <location>
            <position position="326"/>
        </location>
    </feature>
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        <location>
            <begin position="256"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012130"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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            <dbReference type="ARBA" id="ARBA00046386"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042457.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="527" mass="61060" checksum="5A9B7F3724A26463" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQB2</accession>
    <name>A0A673BQB2_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Zgc:174906</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">zgc:174906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="204" mass="23336" checksum="28D8771E75B76143" modified="2020-06-17" version="1">MSGKLWKHIEVLQSHKAKLIDILSSDADFVLQHADSRFLLSPQGYKQIKACRNPSEKVTDLLDHVISRGPDAAHGLLELLKDKELQENFPMLDFIKNVQVNTQSSGRTNHLCSHLVTEKQLMTVARATGRSWREIGILALDIPSVKLDEIMEDHSRHVERVFEMLRCWRNRQRDEATAARLHSLLSHEDLALPKDSIDFLLETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRF4</accession>
    <name>A0A673BRF4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA guanine-7 methyltransferase activating subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RAM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BRF4"/>
    <dbReference type="Ensembl" id="ENSSORT00005045487.1">
        <property type="protein sequence ID" value="ENSSORP00005044365.1"/>
        <property type="gene ID" value="ENSSORG00005020441.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRF4"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028271">
        <property type="entry name" value="RAMAC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48168">
        <property type="entry name" value="RNA GUANINE-7 METHYLTRANSFERASE-ACTIVATING SUBUNIT-LIKE (PSEUDOGENE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48168:SF1">
        <property type="entry name" value="RNA GUANINE-N7 METHYLTRANSFERASE ACTIVATING SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15320">
        <property type="entry name" value="RAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12930" checksum="2EA4EEC876E8EF11" modified="2020-06-17" version="1">MTETQQKLQTYEQMFAHRFSSEDHEYQQYVQRAADPPPTVDDWRGRGGGGGNQRGRDNRHSKWRHFIFHYNMLQINVNFREQFYLVQFCSVLFYLVLCYSIQFYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSC6</accession>
    <name>A0A673BSC6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">steroid 11beta-monooxygenase</fullName>
            <ecNumber evidence="4">1.14.15.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome P450C11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115435537</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030013869.1">
        <property type="nucleotide sequence ID" value="XM_030158009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BSC6"/>
    <dbReference type="Ensembl" id="ENSSORT00005046856.1">
        <property type="protein sequence ID" value="ENSSORP00005045701.1"/>
        <property type="gene ID" value="ENSSORG00005020973.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435537"/>
    <dbReference type="CTD" id="791124"/>
    <dbReference type="InParanoid" id="A0A673BSC6"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004507">
        <property type="term" value="F:steroid 11-beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF1">
        <property type="entry name" value="CYTOCHROME P450 11B2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="9 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 16" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="559" mass="62685" checksum="93FBD5EAD266ECC3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BT40</accession>
    <name>A0A673BT40_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BT40"/>
    <dbReference type="Ensembl" id="ENSSORT00005047146.1">
        <property type="protein sequence ID" value="ENSSORP00005045991.1"/>
        <property type="gene ID" value="ENSSORG00005021085.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BT40"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002104">
        <property type="entry name" value="Si:ch211-266d19.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001156">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000562">
        <property type="entry name" value="Zinc finger protein 786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF47">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="310" mass="35492" checksum="4DC812ACFD83814E" modified="2020-06-17" version="1">MVKEEPPLEQQERSLSLDQVDPEPLHIKEEENLWTNQEGEQLQELEEADITKFPSTPPHVKSEYDEYKGHSSQLHQSQTEENKEEPEDGDDYGEPELESIQNNDFPRSDVACQTRQQQCTEDEKTCKFLSVLDTQMTAHPTKKPFSCSVCNKGFVSKSKLTEHMIVHTREKPFSCSVCKKCFALKSNLKKHMVVHTGERLFCCSVCNKGFARNSNLKNHMLLHTGEKPLSCSVCNKGFVHKGDLNRHMLVHTGEKPFSCSVCNKGFGYKHNLEKHLLTHTGKKQSELSFYRHRKTKKREIDGSTNPGNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTL6</accession>
    <name>A0A673BTL6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase subunit gamma-1</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mitochondrial DNA polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">polg</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
            <location evidence="2">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029992744.1">
        <property type="nucleotide sequence ID" value="XM_030136884.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029992745.1">
        <property type="nucleotide sequence ID" value="XM_030136885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BTL6"/>
    <dbReference type="FunCoup" id="A0A673BTL6">
        <property type="interactions" value="856"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047350.1">
        <property type="protein sequence ID" value="ENSSORP00005046191.1"/>
        <property type="gene ID" value="ENSSORG00005021157.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421153"/>
    <dbReference type="CTD" id="5428"/>
    <dbReference type="InParanoid" id="A0A673BTL6"/>
    <dbReference type="OrthoDB" id="5588663at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005760">
        <property type="term" value="C:gamma DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08641">
        <property type="entry name" value="DNA_pol_gammaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000024">
        <property type="entry name" value="DNA polymerase gamma, catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.390:FF:000001">
        <property type="entry name" value="DNA polymerase gamma, catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.390:FF:000002">
        <property type="entry name" value="DNA polymerase gamma, catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002297">
        <property type="entry name" value="DNA-dir_DNA_pol_A_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041336">
        <property type="entry name" value="DNApol_Exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047580">
        <property type="entry name" value="POLG_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267:SF0">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18136">
        <property type="entry name" value="DNApol_Exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00867">
        <property type="entry name" value="DNAPOLG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="15">
        <location>
            <begin position="834"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1198" mass="137356" checksum="883AFB176B82FB5D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUB4</accession>
    <name>A0A673BUB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-related protein Rab</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">zgc:162171</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">The small GTPases Rab are key regulators in vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Lipid-anchor</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BUB4"/>
    <dbReference type="FunCoup" id="A0A673BUB4">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045312.1">
        <property type="protein sequence ID" value="ENSSORP00005044192.1"/>
        <property type="gene ID" value="ENSSORG00005020372.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUB4"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04107">
        <property type="entry name" value="Rab32_Rab38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000222">
        <property type="entry name" value="RAB32, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030697">
        <property type="entry name" value="Rab29/Rab38/Rab32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF39">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="227" mass="25538" checksum="B6C206FC8367D18D" modified="2020-06-17" version="1">MQHERLLKVLVIGDLGVGKTSIIKRYVHQVFSQHYRATIGVDFALKVLNWDHKTVVRLQLWDIAGQERYGNMTRVYYREAVGALVVFDMTRLSTFQAVLKWKGDLDSKVALSNGRPVPTVLLANKCDQRRHGLCPKLPKLENFTKEYGFVGWFETSAKDNTNIDAAITCLVKNIMSAEEEKAGVDAITKSEPEGSVLVLPRFDYDVKERRLSGCSGCTAVKSRDADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BV78</accession>
    <name>A0A673BV78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="13">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">gpd1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Has glycerol-3-phosphate dehydrogenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:11093"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A673BV78"/>
    <dbReference type="Ensembl" id="ENSSORT00005045585.1">
        <property type="protein sequence ID" value="ENSSORP00005044462.1"/>
        <property type="gene ID" value="ENSSORG00005020447.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BV78"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017751">
        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF32">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)], CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="14">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="15">
        <location>
            <begin position="207"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="280"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="39197" checksum="9BEF9856F2CD99CD" modified="2025-10-08" version="2">MCDHHFRVSQQDHSIHTETLKPAQGSAIAKIVGANAAKYDKFDTTVNMWVFEEMVNGRKLTEIINTDHENVKYLPGHKLPPNVVAVPDLTEAAKGADILIFVVPHQFIVRVCDTIKDHIKKDAIGMSLIKGVDASPEGLKLISEVIRAKLGITMTVLMGANIANEVADEKFCETTIGCTDKAHGPMLKELMQTTNFRVTVVEESDVVEICGALKNIVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKVFCTNCPVSPATFLESCGIADLITTCYGGRNRKIGEAFAKTGKTIEQLENELLNGQKLQGPATASEVHQVLTQKNMVAKFPLFTAVYEICFNGHPVTEFISCLQNHPEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BWW9</accession>
    <name>A0A673BWW9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peroxisomal succinyl-coenzyme A thioesterase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115419223</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989731.1">
        <property type="nucleotide sequence ID" value="XM_030133871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BWW9"/>
    <dbReference type="Ensembl" id="ENSSORT00005047449.1">
        <property type="protein sequence ID" value="ENSSORP00005046290.1"/>
        <property type="gene ID" value="ENSSORG00005021205.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419223"/>
    <dbReference type="InParanoid" id="A0A673BWW9"/>
    <dbReference type="OrthoDB" id="6347013at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000024">
        <property type="entry name" value="acyl-coenzyme A thioesterase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2240">
        <property type="entry name" value="Acyl-CoA thioester hydrolase/BAAT N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016662">
        <property type="entry name" value="Acyl-CoA_thioEstase_long-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014940">
        <property type="entry name" value="BAAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006862">
        <property type="entry name" value="Thio_Ohase/aa_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042490">
        <property type="entry name" value="Thio_Ohase/BAAT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824:SF36">
        <property type="entry name" value="ACYL-COA THIOESTERASE 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08840">
        <property type="entry name" value="BAAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04775">
        <property type="entry name" value="Bile_Hydr_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016521">
        <property type="entry name" value="Acyl-CoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase" evidence="3">
        <location>
            <begin position="59"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="BAAT/Acyl-CoA thioester hydrolase C-terminal" evidence="4">
        <location>
            <begin position="258"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR016521-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="469" mass="51387" checksum="8AFF50180260E596" modified="2020-06-17" version="1">MSRNTLRPLTVKMITSLLRVHRKLSSLNPAVGAVCGVRWSSGLNRPPLLSAAPARALMDEQIIIKGRFLPPHCPVTVCAQLHSDDGDLWQSFAHYNTNADGTVNLASDHSVGGSYLGCEPMGLFWGLEPAPGSREGLRLRKKNVETPYVVHMSLLEGHVSQREEQSVELAVATTERWYMAPGVKRKDIRQNGVVGTLFLPPGPGPFPAMLDMWGMGGGLMEYRSALLASRGYAAFSLAYFGHKDLPGPQTTINVGDAYFKSAFQFLQDHHQICANRVGIIGLSFGVYLTLRMAAHIGVKASCLICINGPVGSVLDLVDADGRCTAFEESSQKHWSFDERGYVSFRDISSPGNFNPENKLKLENISCPLMWVVGEDDMSASSIENADVMEETLKASGKAHLFTRLSYPGAGHLIEPPYSPNARLSLWSIKPVKYITIWGGYPAPHAAAQEDAWRKILDFLDTNLRGFGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXG8</accession>
    <name>A0A673BXG8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger matrin-type protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">zmat2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing as a component of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the spliceosome B complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BXG8"/>
    <dbReference type="FunCoup" id="A0A673BXG8">
        <property type="interactions" value="1150"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046959.1">
        <property type="protein sequence ID" value="ENSSORP00005045804.1"/>
        <property type="gene ID" value="ENSSORG00005021024.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BXG8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000282">
        <property type="entry name" value="Zinc finger, matrin-type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040107">
        <property type="entry name" value="Snu23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="11">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="144"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="206" mass="24255" checksum="1B86D272D610E8E4" modified="2020-06-17" version="1">MAASGSGVSGQFCKNDFRRKWDKDEYENLAQKRLAEERERDRRDGKTAPPVKRDLLRHRDYKVDLESKLGKTIVITKTTPQAEMGGYYCNVCDCVVKDSINFLDHINGKKHQRNLGMSMRVERSSLDQVKKRFEVNKKKMEEKQKEYDFEERMKELREEEEKAKAYKKEKQKERKRRAEEDIDLEEDDEMAAVMGFSGFGSSKKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZ80</accession>
    <name>A0A673BZ80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier family 35 member B4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">slc35b4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029992910.1">
        <property type="nucleotide sequence ID" value="XM_030137050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BZ80"/>
    <dbReference type="FunCoup" id="A0A673BZ80">
        <property type="interactions" value="885"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046583.1">
        <property type="protein sequence ID" value="ENSSORP00005045433.1"/>
        <property type="gene ID" value="ENSSORG00005020877.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421254"/>
    <dbReference type="CTD" id="84912"/>
    <dbReference type="InParanoid" id="A0A673BZ80"/>
    <dbReference type="OrthoDB" id="999962at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005462">
        <property type="term" value="F:UDP-N-acetylglucosamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005464">
        <property type="term" value="F:UDP-xylose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF4">
        <property type="entry name" value="NUCLEOTIDE SUGAR TRANSPORTER SLC35B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025572790" description="Solute carrier family 35 member B4" evidence="10">
        <location>
            <begin position="21"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="295"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="333" mass="37374" checksum="CAA2AF9B81186234" modified="2020-06-17" version="1">MGPVFATVLVFVGCCSNVVSLELLVREFPGCGNIVTFAQFLFIALEGFIFEANFGRKKPAIPISNYVIMVTMFFTVSVINNYALNFNIAMPLHMIFRSGSLIANMILGIIILKKRYSASKYLSIALVSAGIFICTIMSAKQVNVANEGSEEQGFYAFMHWLIGIAMLTFALLMSARMGIFQETLYKQYGKHSKEALFYNHCLPLPGFLILSTNIYKHCVHFSQSTPVMVPVVGLTVPIMWIYLFINVITQYVCIRGVFILTTECTSLTVTLVVTLRKFLSLIFSILYFQNPFTTWHWVGTAVVFLGTLLYTEVWSSVQAALGRPGIKEQKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C2L8</accession>
    <name>A0A673C2L8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="38">G-protein coupled estrogen receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="39">G protein-coupled estrogen receptor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="40">G-protein coupled receptor 30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="44" type="primary">LOC115439669</name>
    </gene>
    <organism evidence="44 45">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="44" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="44" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="44" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell projection</location>
            <location evidence="10">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="6">Dendritic spine membrane</location>
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        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="37">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="41">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030163541.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_030163542.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030019403.1">
        <property type="nucleotide sequence ID" value="XM_030163543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C2L8"/>
    <dbReference type="Ensembl" id="ENSSORT00005047739.1">
        <property type="protein sequence ID" value="ENSSORP00005046578.1"/>
        <property type="gene ID" value="ENSSORG00005021323.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439669"/>
    <dbReference type="InParanoid" id="A0A673C2L8"/>
    <dbReference type="OrthoDB" id="5957382at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
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    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:early endosome"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:mitochondrial membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0030284">
        <property type="term" value="F:nuclear estrogen receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:apoptotic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071392">
        <property type="term" value="P:cellular response to estradiol stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047143">
        <property type="entry name" value="GPER1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182 AND ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="35" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="34" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="36" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="30" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="19" id="KW-0221">Differentiation</keyword>
    <keyword evidence="28" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0967">Endosome</keyword>
    <keyword evidence="25 41" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="24" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="27 42" id="KW-0472">Membrane</keyword>
    <keyword evidence="26" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="32" id="KW-0539">Nucleus</keyword>
    <keyword evidence="33" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29 41" id="KW-0675">Receptor</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0770">Synapse</keyword>
    <keyword evidence="31 41" id="KW-0807">Transducer</keyword>
    <keyword evidence="16 41 42" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="22 42" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
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        <location>
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            <end position="174"/>
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    <feature type="transmembrane region" description="Helical" evidence="42">
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            <end position="216"/>
        </location>
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        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="43">
        <location>
            <begin position="59"/>
            <end position="296"/>
        </location>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046578.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
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        </source>
    </evidence>
    <sequence length="377" mass="43163" checksum="E1ECF7E961082E90" modified="2020-06-17" version="1">MDMDYEVTAGENLTVWKNGTWSVVSGFLQAASENHYHYVISIFFSGLYISFLFPVGFIGNILILTVNLTHRGRLSAPDLYFVNLAVADLALVFDSLIEVFNLKQGYYDKAGLCTFMNLFQHVNMYSSVFFLTWMSFDRFVALTGSMGRRMPRPRLSCSLIWVSSSMLTVLPFAVSQAQHAGDLHFCFANVTQIQWLEVILGFLMPFCILGLCYWRIAQVLQRSQRQQRPRRQKALRMISAAVFVFFLCWLPENIFVSIHLLRQDADGSTLWQDYPLTGHAVRLAAFSNSCLNPLIYSFLGETFLDKLRLFLQEKTRWTKLHRSASRKSIYIPAARMCRCGTTPDTHSNCKTLSKAFIFPSDEELDTAEHTDDGQLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C3B8</accession>
    <name>A0A673C3B8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Storkhead box 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">stox1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSSORG00005021803.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C3B8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019391">
        <property type="entry name" value="Storkhead-box_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040126">
        <property type="entry name" value="STOX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437:SF1">
        <property type="entry name" value="STORKHEAD-BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437">
        <property type="entry name" value="WINGED HELIX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10264">
        <property type="entry name" value="WHD_Storkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Winged helix Storkhead-box1" evidence="2">
        <location>
            <begin position="118"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="798"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="801"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="983" mass="110323" checksum="F386D82CCBD88BDD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5X2</accession>
    <name>A0A673C5X2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinase</fullName>
            <ecNumber evidence="7">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">itpka</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 1,4,5-trisphosphate + ATP = 1D-myo-inositol 1,3,4,5-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11020"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.127"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:11021"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A673C5X2"/>
    <dbReference type="FunCoup" id="A0A673C5X2">
        <property type="interactions" value="414"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049846.1">
        <property type="protein sequence ID" value="ENSSORP00005048654.1"/>
        <property type="gene ID" value="ENSSORG00005022159.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C5X2"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.160:FF:000001">
        <property type="entry name" value="Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF55">
        <property type="entry name" value="INOSITOL-TRISPHOSPHATE 3-KINASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="449" mass="51066" checksum="F51A192FAD765F0E" modified="2020-06-17" version="1">MPKECRRKTSKDLGLSGINEGSRSERRSAQKSSSQICNERIHQTPSPESAPPTLMETRRVPQVTITPEGGGTSREMQQEDWDDAVDGTLRRKLSNSSISSTGSSAVESEDDLLSDNESKSKGIVTLEHLVDPGEPKPWWKLKTIVHWPFSATQRRKLNWVQLAGHKGNFKAADEGTILKKFSENEMQCFEKLRGDALLPFVPAYHGVVEKDGESFLHMADLLANFDLPNVMDCKMGVRTYLEEELVRARERPKPREDLYRKMVEVDSEGPTPQEHSQRGVTKPRYMQWRETMSSTNTLGFRIEGIKKCDGTCRTDFKKTRSKQDVIQVFSDFVGGNVNILKSYLNRLAEVRQALKMSEFFKRHEVIGSSLLFIHDRTGNAQVWIIDFGKTTALPEGQMLHHDIPWQEGNREDGYLWGLENLIHTLESVNVQETEEETCCSSTKENTQMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C6T4</accession>
    <name>A0A673C6T4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MARCKS-related protein 1-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">marcksl1a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MARCKS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C6T4"/>
    <dbReference type="FunCoup" id="A0A673C6T4">
        <property type="interactions" value="392"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049154.1">
        <property type="protein sequence ID" value="ENSSORP00005047968.1"/>
        <property type="gene ID" value="ENSSORG00005021898.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C6T4"/>
    <dbReference type="OrthoDB" id="9948538at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002101">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353:SF8">
        <property type="entry name" value="MARCKS-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353">
        <property type="entry name" value="MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02063">
        <property type="entry name" value="MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00963">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00826">
        <property type="entry name" value="MARCKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="115"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="207" mass="20470" checksum="6AE2A723CFB77039" modified="2020-06-17" version="1">MGAHMSKGGVVVQGKAAADPAAAKANGQENGHVKTNGDVSAKPDGEVAASDGNGTAEPAKEGEAGAGDAIEPAPAAEGEAAKAEGEAAKDGKKKKKFSLKNSFKFKGISLKKSKKGSEEGKEEATSPTTEDKPEENGHAAKETKEDTPAAEAKEGEAAAAEPAAAAPEEETKAAEEAPPTEAAPAEEAAAPAEGTTPAASEGEAKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBF0</accession>
    <name>A0A673CBF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115439299</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBF0"/>
    <dbReference type="Ensembl" id="ENSSORT00005050659.1">
        <property type="protein sequence ID" value="ENSSORP00005049458.1"/>
        <property type="gene ID" value="ENSSORG00005022240.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBF0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF233">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="565"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="608"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="682" mass="76348" checksum="6A188316FCA722CF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKV5</accession>
    <name>A0A673CKV5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Growth arrest-specific 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gas1b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CKV5"/>
    <dbReference type="Ensembl" id="ENSSORT00005055189.1">
        <property type="protein sequence ID" value="ENSSORP00005053919.1"/>
        <property type="gene ID" value="ENSSORG00005024213.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CKV5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039596">
        <property type="entry name" value="GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16840:SF7">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16840">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025431743" evidence="6">
        <location>
            <begin position="29"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="7">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="7">
        <location>
            <begin position="123"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="241" mass="26791" checksum="E58BC4B4F3DA416D" modified="2020-06-17" version="1">MASRAMLMARTEVLICSVFILMVGLCVASNNHGRRLVCWKAILKCHGEPECHYAYDQYLFACASVLSGERKKCPSHCISSLIQLNLTQSGPGLEDCDCAKDPVCRGTKQAIEPCLPRTSTMGCTEARRQCERDQPCSSALNDYLIHCRKLFGGDMCSDGCRRVIANLRSIPKGQQLDTCVCDGTERNICEYIKVSMKTYCSDPGDRFTGSGFSDSEDTEDTDDEREALRVILMTLYTLVFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CP97</accession>
    <name>A0A673CP97_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">trypsin</fullName>
            <ecNumber evidence="10">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115435066</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A673CP97"/>
    <dbReference type="Ensembl" id="ENSSORT00005056442.1">
        <property type="protein sequence ID" value="ENSSORP00005055164.1"/>
        <property type="gene ID" value="ENSSORG00005024652.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CP97"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000008">
        <property type="entry name" value="Cationic trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000122">
        <property type="entry name" value="Chymotrypsin-like elastase family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF68">
        <property type="entry name" value="TRYPSIN-3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025407119" description="trypsin" evidence="12">
        <location>
            <begin position="16"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="22"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="247" mass="27262" checksum="106B9C08672C0E89" modified="2020-06-17" version="1">MIGLIVLTVLGAAAAAPLDDKIVGGYECTPYTNQWQVSINIGYHYCGGSLINDQWIVSAAHCWQNPYSQIAVLGDHHIWVHEGTEQYMSVDAIYWHELYDYKTLDYDIMLMKLAHPVTVNEYVKPIALPKACPNPGDMCKVTGWGNIYTDSVFNPFQLRCVEVPIVSHKDCDVSYPGQITERMLCAGYPEGGKDACQGDSGGPLVCNGVLQGIVSWGYGCAQPNYPGVYTKVCALLPWINDILARYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CPJ7</accession>
    <name>A0A673CPJ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Complement component C9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">c9</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Constitutes the pore-forming subunit of the MAC complex: during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homooligomer; about 20 C9 chains oligomerize to give rise to a huge beta-barrel that forms a 100 Angstrom diameter pore in target membranes. Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CPJ7"/>
    <dbReference type="FunCoup" id="A0A673CPJ7">
        <property type="interactions" value="938"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055188.1">
        <property type="protein sequence ID" value="ENSSORP00005053918.1"/>
        <property type="gene ID" value="ENSSORG00005024207.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CPJ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006957">
        <property type="term" value="P:complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF3">
        <property type="entry name" value="COMPLEMENT COMPONENT C9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0179">Complement alternate pathway</keyword>
    <keyword evidence="13" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="11" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="16 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="19" id="KW-1053">Target membrane</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="MACPF" evidence="24">
        <location>
            <begin position="141"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="558"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="572"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00093294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00093512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="588" mass="66185" checksum="9705A563F0709D6C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CTB6</accession>
    <name>A0A673CTB6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115425406</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029998812.1">
        <property type="nucleotide sequence ID" value="XM_030142952.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029998813.1">
        <property type="nucleotide sequence ID" value="XM_030142953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CTB6"/>
    <dbReference type="Ensembl" id="ENSSORT00005057088.1">
        <property type="protein sequence ID" value="ENSSORP00005055802.1"/>
        <property type="gene ID" value="ENSSORG00005024872.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425406"/>
    <dbReference type="InParanoid" id="A0A673CTB6"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000009">
        <property type="entry name" value="Rieske Fe-S domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054716">
        <property type="entry name" value="Sol_Rieske_ferrdox_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF16">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22543">
        <property type="entry name" value="Rieske_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Rieske" evidence="10">
        <location>
            <begin position="29"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="176" mass="19733" checksum="3E8CDD80C347A654" modified="2020-06-17" version="1">MLSSEEDISQSSSSSLSPPSSSTPPLSTHFIGRKEDIVKAGRVTKLVNGCRDVLVLHHDGQLYAMDMRCYHAGGALEHGDIEEFNGRLCIVCPWHKYKITLAEGEGLYQAVDNPTVKPLRTYWRSKGVKQRIHKVTEVNGDVYVTLNDLSEAIESDNYQTEKYRSVFLKGHTQHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y249</accession>
    <name>A0A672Y249_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein 134</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tmem134</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM134/TMEM230 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987150.1">
        <property type="nucleotide sequence ID" value="XM_030131290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y249"/>
    <dbReference type="Ensembl" id="ENSSORT00005000063.1">
        <property type="protein sequence ID" value="ENSSORP00005000062.1"/>
        <property type="gene ID" value="ENSSORG00005000039.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417295"/>
    <dbReference type="CTD" id="80194"/>
    <dbReference type="InParanoid" id="A0A672Y249"/>
    <dbReference type="OrthoDB" id="10048380at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039714">
        <property type="entry name" value="TMEM134"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008590">
        <property type="entry name" value="TMEM_230/134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13558">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13558:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05915">
        <property type="entry name" value="TMEM_230_134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="22880" checksum="D818CB89FD99D0B8" modified="2020-06-17" version="1">MATQFSIDDAFVLEGDEEGAVSDGEMEGWKAREKDREGGEMTFGPLTFSKPQTHPSPAAAASSPEHSNLKYQNLENEDAVGNNGNSSFNNFFKISDPSTLSYCSSQWSFSTLSSVTQLSAHCCGWVSHPLIKTNRKVVLASFLLLITGVALIFTGVVIQLNPNAGVSSAIFFVPGFLLFIPGVYHVIYISCAVRGRRGFKLFYLPYFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5M3</accession>
    <name>A0A672Y5M3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein FAM8A1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115435222</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030013360.1">
        <property type="nucleotide sequence ID" value="XM_030157500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y5M3"/>
    <dbReference type="Ensembl" id="ENSSORT00005001851.1">
        <property type="protein sequence ID" value="ENSSORP00005001797.1"/>
        <property type="gene ID" value="ENSSORG00005001122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435222"/>
    <dbReference type="InParanoid" id="A0A672Y5M3"/>
    <dbReference type="OrthoDB" id="10061042at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039871">
        <property type="entry name" value="FAM8A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13659">
        <property type="entry name" value="AUTOSOMAL HIGHLY CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13659:SF6">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 8 MEMBER A1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="7">
        <location>
            <begin position="162"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="331" mass="36900" checksum="DAA228A72544FEA2" modified="2020-06-17" version="1">MADEENTNMEVDKEKNKPFSNKAMPLFDEVIQRIGSQNNVEHKTDAGESCATAEYCEKLQAWMWQYYTGYVSWQSWLAASAMAYPYYLQSGNGTSMPPLDLNSQNWFNSPLGLPLSPYPPAAASPSSRAGEAAAGAAVPTQPQQHPQENGNAQRPGREYSIPSPLQRLLAEMVDFFILFFIKATIIISIMHLSGIKDVSKFAMHFIVEEIDEDTSMEELQKMMLVALVYRILVCFYEIVCIWGAGGATPGKFLMGLRVVTCDSSVLVQPNRVFVVPATNVSLSASTVRALNKNFSIAFFFPAFITLLFFQHNRTVYDMVAGTIVVKRSRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8Q6</accession>
    <name>A0A672Y8Q6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y8Q6"/>
    <dbReference type="Ensembl" id="ENSSORT00005003239.1">
        <property type="protein sequence ID" value="ENSSORP00005003143.1"/>
        <property type="gene ID" value="ENSSORG00005001937.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8Q6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="308"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="840" mass="94579" checksum="47E210FE22225943" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9F6</accession>
    <name>A0A672Y9F6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y9F6"/>
    <dbReference type="Ensembl" id="ENSSORT00005000464.1">
        <property type="protein sequence ID" value="ENSSORP00005000439.1"/>
        <property type="gene ID" value="ENSSORG00005000307.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y9F6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd20254">
        <property type="entry name" value="CASIMO1_SMIM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047133">
        <property type="entry name" value="SMIM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031671">
        <property type="entry name" value="SMIM5/18/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37344">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37344:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15831">
        <property type="entry name" value="SMIM5_18_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="71" mass="8019" checksum="FB4AF5DBEB66C0EF" modified="2020-06-17" version="1">LGATKMDAREETLNLLNRIWIKLQHLPNANPIELGAFFILLTFILVVLLMMILTCVTCCCCRKARNKGSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y9S5</accession>
    <name>A0A672Y9S5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin-protein ligase TRIM33</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RING-type E3 ubiquitin transferase TRIM33</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Transcription intermediary factor 1-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Tripartite motif-containing protein 33</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">trim33</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029994795.1">
        <property type="nucleotide sequence ID" value="XM_030138935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y9S5"/>
    <dbReference type="FunCoup" id="A0A672Y9S5">
        <property type="interactions" value="1413"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000715.1">
        <property type="protein sequence ID" value="ENSSORP00005000689.1"/>
        <property type="gene ID" value="ENSSORG00005000405.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422558"/>
    <dbReference type="CTD" id="51592"/>
    <dbReference type="InParanoid" id="A0A672Y9S5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16766">
        <property type="entry name" value="RING-HC_TIF1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000123">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000246">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM33 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000074">
        <property type="entry name" value="Tripartite motif containing 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 23" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="13 24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
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            <end position="205"/>
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            <end position="280"/>
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            <end position="336"/>
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            <end position="414"/>
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            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
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            <begin position="67"/>
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        <source>
            <dbReference type="PROSITE" id="PS50119"/>
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    <sequence length="1177" mass="127827" checksum="F3FEB989DFD51809" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCE3</accession>
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            <authorList>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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</entry>
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            <taxon>Apogonoidei</taxon>
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            <authorList>
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            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
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    <dbReference type="PANTHER" id="PTHR33332">
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    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="33187" checksum="5367F664DA9E9DB5" modified="2020-06-17" version="1">MLPLGEIFKCHNISFHCYADDTQIHLPLRPDDPRSLAAVSDCLNDVSRWMAQNFLQLNSSKSEVILFGPPNSTIAIKNSLGPLSSNITPTARNLGVIFDSDLSFQLHINKVIQSCFLQLRTISKIKPIVPHHNLETIIHSFIFSRLDYCNSLLSGISQKSLSRLQLVQNAAARLLTGFSRHHHITPILASLHWLPVCFRIDFKILLITFKARMGLAPSYIQEMLVDYEPARSLRSSGRGLLAVPKSRLKSKGDRAFSIRAPQLWNGLPEEIRLAESVTSFKSLLKTHFYRIAFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIS1</accession>
    <name>A0A672YIS1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposable element Tc3 transposase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
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    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12301" checksum="540A6185AA899A24" modified="2025-10-08" version="2">MLLHPVGDHSHSPFAPLLWKKVLFSDEKKFNLDGPDGFQRYWHDKEIPPEIFSTQHSGGGSIMIWGAFSFSGTLELQVVQGRQTAAGYVQMLQRASLMPEGPRLCGSSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YK81</accession>
    <name>A0A672YK81_9TELE</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YK81"/>
    <dbReference type="Ensembl" id="ENSSORT00005005062.1">
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        <property type="gene ID" value="ENSSORG00005002932.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF35">
        <property type="entry name" value="GERM CELL-SPECIFIC GENE 1-LIKE PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="193" mass="21610" checksum="3E5220FA2A502C4D" modified="2020-06-17" version="1">CCVSSVVYPLCAGVLWLSVVSEVLYILLLVVGFSLMCLELFHSSNVIDGLKLNAFAAVFTVFQITVSLGPEDWRPHSWDYGWSFCMAWGSFTCCMAASVTTLNSYTKTVIEFRHKRKLFEQGLREEQNYLDQEAFHYFRDRSLQSISSTVEVYPGHGGTRAGMGVGTSAVHLEEKKSLLVLSECFISLCRGCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLJ3</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YLJ3"/>
    <dbReference type="Ensembl" id="ENSSORT00005005614.1">
        <property type="protein sequence ID" value="ENSSORP00005005449.1"/>
        <property type="gene ID" value="ENSSORG00005003247.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLJ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF22">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="27"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
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            <end position="232"/>
        </location>
    </feature>
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        <location>
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            <end position="336"/>
        </location>
    </feature>
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        <location>
            <begin position="367"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="558"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="53"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="66"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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            <end position="325"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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            <end position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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            <end position="457"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
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        </location>
    </feature>
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        <location>
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            <end position="447"/>
        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="687" mass="73215" checksum="0177DF2C1BC8AE44" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSE6</accession>
    <name>A0A672YSE6_9TELE</name>
    <protein>
        <recommendedName>
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            <ecNumber evidence="5">1.1.1.n12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">17-beta-hydroxysteroid dehydrogenase 8</fullName>
        </alternativeName>
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            <fullName evidence="23">3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Estradiol 17-beta-dehydrogenase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Testosterone 17-beta-dehydrogenase 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">hsd17b8</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>17beta-estradiol + NAD(+) = estrone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:24613"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:24614"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>17beta-hydroxy-5alpha-androstan-3-one + NAD(+) = 5alpha-androstan-3,17-dione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15994"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:41993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a (3R)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32711"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.n12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:32712"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14929"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.239"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14930"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Steroid biosynthesis; estrogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Heterotetramer with CBR4; contains two molecules of HSD17B8 and CBR4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.239" evidence="21"/>
    <dbReference type="EC" id="1.1.1.62" evidence="14"/>
    <dbReference type="EC" id="1.1.1.n12" evidence="5"/>
    <dbReference type="RefSeq" id="XP_030003343.1">
        <property type="nucleotide sequence ID" value="XM_030147483.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030003344.1">
        <property type="nucleotide sequence ID" value="XM_030147484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YSE6"/>
    <dbReference type="Ensembl" id="ENSSORT00005007711.1">
        <property type="protein sequence ID" value="ENSSORP00005007432.1"/>
        <property type="gene ID" value="ENSSORG00005004239.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115428436"/>
    <dbReference type="CTD" id="7923"/>
    <dbReference type="InParanoid" id="A0A672YSE6"/>
    <dbReference type="OrthoDB" id="294295at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047035">
        <property type="term" value="F:testosterone dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008210">
        <property type="term" value="P:estrogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05333">
        <property type="entry name" value="BKR_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000231">
        <property type="entry name" value="Estradiol 17-beta-dehydrogenase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009466">
        <property type="entry name" value="PRK12826.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF83">
        <property type="entry name" value="(3R)-3-HYDROXYACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="28">
        <location>
            <begin position="10"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00065174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00066822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00077835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00081419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00081936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00083097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00083258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="256" mass="26699" checksum="322EAAE5BA5F9BDC" modified="2020-06-17" version="1">MAASTRLISRLTLVTGGGSGIGRAVCQRFASEGASVVVADISPDSASETLEKLQTDHRGQGHMAAEVDVASKESVKRLITNVQSLYFQPPSVCVNAAGITQDDFLLNMDEDQFDRVIQINLKGSFLVTQAVAQALVACGAPKGSVITVGSIVGKVGNIGQANYAASKAGVEGLTRSAAKELSRFGIRCNCVLPGFISTPMTDKVPEKVINKMMSLVPLGRMGEPAEVADVCAFLASDDSRYITGASLEVTGGLFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSY3</accession>
    <name>A0A672YSY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Aminopeptidase</fullName>
            <ecNumber evidence="17">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">erap2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endomembrane system</location>
            <topology evidence="13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 17">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A672YSY3"/>
    <dbReference type="Ensembl" id="ENSSORT00005007965.1">
        <property type="protein sequence ID" value="ENSSORP00005007684.1"/>
        <property type="gene ID" value="ENSSORG00005004326.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YSY3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000016">
        <property type="entry name" value="Glutamyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000003">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF239">
        <property type="entry name" value="ENDOPLASMIC RETICULUM AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 17" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 17" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 15 17" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025656796" description="Aminopeptidase" evidence="18">
        <location>
            <begin position="22"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="21">
        <location>
            <begin position="56"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="19">
        <location>
            <begin position="277"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="20">
        <location>
            <begin position="598"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="926" mass="105959" checksum="A79414A85336533D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YTY3</accession>
    <name>A0A672YTY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Interleukin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115426502</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the IL-1 family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSSORT00005008328.1">
        <property type="protein sequence ID" value="ENSSORP00005008043.1"/>
        <property type="gene ID" value="ENSSORG00005004506.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005149">
        <property type="term" value="F:interleukin-1 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001660">
        <property type="term" value="P:fever generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048246">
        <property type="term" value="P:macrophage chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neutrophil activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23296">
        <property type="entry name" value="beta-trefoil_IL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interleukin-1 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000975">
        <property type="entry name" value="IL-1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078:SF30">
        <property type="entry name" value="INTERLEUKIN-1 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078">
        <property type="entry name" value="INTERLEUKIN-1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00340">
        <property type="entry name" value="IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00264">
        <property type="entry name" value="INTERLEUKIN1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01359">
        <property type="entry name" value="INTRLEUKIN1B"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01357">
        <property type="entry name" value="INTRLEUKN1AB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00125">
        <property type="entry name" value="IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-0497">Mitogen</keyword>
    <keyword evidence="8" id="KW-0666">Pyrogen</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="256" mass="28938" checksum="B13F14A88BD6E516" modified="2020-06-17" version="1">MSLQLLLNRKMESGMICSVSQAWSPKLPKGIDLEISQHPLKMRSVVNLVIAIERLKQSASESVLDTDFKDENLLSIMLDSIVEETLVLERGSAPPPQFNRTLEYPCSVTDHQQRSLVLVQNSMELHAVMLQGGSDSRKVHLNMSTYVNPASSIEARPVALGIKGTNLYMSCHKEGEEPTLHLETVETKDNLANISTESDMVRFIFYKQDTGLNISTLRSARYPEWYISTAQQDNKPVQMCQETSSRHRIFNLQRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YYT7</accession>
    <name>A0A672YYT7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Extracellular calcium-sensing receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115431813</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YYT7"/>
    <dbReference type="FunCoup" id="A0A672YYT7">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010086.1">
        <property type="protein sequence ID" value="ENSSORP00005009760.1"/>
        <property type="gene ID" value="ENSSORG00005005219.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YYT7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025379495" evidence="13">
        <location>
            <begin position="32"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="621"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="654"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="745"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="778"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="810"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="584"/>
            <end position="848"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="848" mass="94472" checksum="DB3A7D37FF5C026D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z0X1</accession>
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        <submittedName>
            <fullName evidence="12">Lectin, mannose-binding, 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="12" type="primary">lman1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
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        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
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        <property type="entry name" value="LECTIN, MANNOSE BINDING 1 LIKE"/>
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    </dbReference>
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        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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        <property type="entry name" value="L_LECTIN_LIKE"/>
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    </dbReference>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
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    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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    <feature type="chain" id="PRO_5025643560" evidence="10">
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        <location>
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            <end position="293"/>
        </location>
    </feature>
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        <location>
            <begin position="294"/>
            <end position="314"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="534" mass="60643" checksum="0983F4F95D892F4C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z1D2</accession>
    <name>A0A672Z1D2_9TELE</name>
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        <submittedName>
            <fullName evidence="20">Opsin 1 (cone pigments), short-wave-sensitive 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115419156</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
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    <dbReference type="Ensembl" id="ENSSORT00005010856.1">
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        <property type="gene ID" value="ENSSORG00005005684.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z1D2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="Opsin"/>
    </dbReference>
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        <property type="entry name" value="Opsin_blue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00574">
        <property type="entry name" value="OPSINBLUE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0157">Chromophore</keyword>
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    <keyword evidence="10 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 17" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="5 17" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="15 17" id="KW-0807">Transducer</keyword>
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        <location>
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        <location>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002881"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="PROSITE" id="PS50262"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010519.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="369" mass="41079" checksum="59582E843C3AFFA4" modified="2020-06-17" version="1">MRTDRGMELPEDFWIPIPLDTNNITSLSPFLVPQDHLGNVGVFYSMAGFMFFVFVVGTAINALTIACTVQYKKLRSHLNYILVNLAIANLIVSCVGSFTACCSFASRYFIFGPLACKVEGFMATLGGMVSLWSLAVVAFERWLVICKPLGNFIFKPDHAIACCVFTWIFALMASVPPLCGWSRYIPEGLQCSCGPDWYTTNNKYNNESYVMFLFCFCFAVPFATIVFCYAQLLFTMKMAARAQAESASTQKAEKEVTRMVVIMVVGFLVCWMPYASFAVWVVNNRGQPFDLRLATIPSCFSKASAVYNPYFCCLCSCIFMFLNDCRFMFSSSFPSQFRGCMMKMLGMGGDDDETSSSASVTEVSKVGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z425</accession>
    <name>A0A672Z425_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">guanylate cyclase</fullName>
            <ecNumber evidence="2">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gucy1a2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
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    <dbReference type="OrthoDB" id="6127067at2759"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Haem NO binding associated domain"/>
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        <property type="entry name" value="Heme_NO-bd"/>
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        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
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        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT ALPHA-2"/>
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        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2"/>
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        <property type="entry name" value="Guanylate_cyc"/>
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    </dbReference>
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        <property type="entry name" value="HNOB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
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    </dbReference>
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        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="11">
        <location>
            <begin position="589"/>
            <end position="716"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <end position="89"/>
        </location>
    </feature>
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            <begin position="220"/>
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        </location>
    </feature>
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            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
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        <location>
            <begin position="530"/>
            <end position="561"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
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            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="804" mass="89045" checksum="3929B7E017E35F02" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4S1</accession>
    <name>A0A672Z4S1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein ubi-d4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">dpf2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the requiem/DPF family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005011693.1"/>
        <property type="gene ID" value="ENSSORG00005006243.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071565">
        <property type="term" value="C:nBAF complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15691">
        <property type="entry name" value="PHD1_DPF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15530">
        <property type="entry name" value="PHD2_d4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003699">
        <property type="entry name" value="D4, zinc and double PHD fingers family 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000005">
        <property type="entry name" value="zinc finger protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025750">
        <property type="entry name" value="DPF1-3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888">
        <property type="entry name" value="HL01030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888:SF7">
        <property type="entry name" value="ZINC FINGER PROTEIN UBI-D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14051">
        <property type="entry name" value="DPF1-3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="223"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="284"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="341"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="248"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="407" mass="46128" checksum="A6CB34FD0AD1A2AE" modified="2020-06-17" version="1">MEQCHNYNARLCAERSVRMPFLDSQTGVAQSNCYIWMEKRHRGPGMAPGQLYTYPSRRWRKRRRTHPPEDPRLLFPPVKSEIDLGLKKDSLLSSDGSSLEALLKGDSLDKRTTTEVRGSEEDSNLSDYTGSLNPAARIRKVLILFYLHVVKISKRMINCLILPSLLLKQRILEPDDFLDDLDDEDYEEDTPKRRGKGKGKGRGVGSNRKKLDAAALEDRDKPYACDICGKRYKNRPGLSYHYAHSHLAEEEGEEKEEMEMEPTLPLPDEPKTPKKGPDGLALPNNYCDFCLGDSKTNHKTGQSEELVSCSDCGRSGHPSCLQFTPVMMAAVKTYRWQCIECKCCNMCGTSENDDQLLFCDDCDRGYHMYCLNPPMSEPPEGSWSCHLCLDLLKDKASIYQNQNAPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZA13</accession>
    <name>A0A672ZA13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Gasdermin Eb</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">gsdmeb</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the gasdermin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZA13"/>
    <dbReference type="FunCoup" id="A0A672ZA13">
        <property type="interactions" value="984"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014603.1">
        <property type="protein sequence ID" value="ENSSORP00005014181.1"/>
        <property type="gene ID" value="ENSSORG00005007263.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZA13"/>
    <dbReference type="OrthoDB" id="8815334at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040460">
        <property type="entry name" value="Gasdermin_pore"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041263">
        <property type="entry name" value="Gasdermin_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042377">
        <property type="entry name" value="GSDME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207:SF3">
        <property type="entry name" value="DEAFNESS, AUTOSOMAL DOMINANT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207">
        <property type="entry name" value="NONSYNDROMIC HEARING IMPAIRMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04598">
        <property type="entry name" value="Gasdermin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17708">
        <property type="entry name" value="Gasdermin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1210">Necrosis</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Gasdermin pore forming" evidence="12">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Gasdermin PUB" evidence="13">
        <location>
            <begin position="268"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="50736" checksum="F76CF02BE1BA92C7" modified="2020-06-17" version="1">MFATATRNFVEEVDPGGLLIPVSSLNDTVTLLTVVVKRKRVWFWRKSKYIPTDFNLNDLLTGDTPITPVVIESDFITYNGSYGDNIQGTIGAQFVHTNVNLEGKDSSKLQSSFGSLKKEEVDVQKLLKASKERVLDMSHCLIQQTKEKHRRTFGIVKECIVTTQPCSVIEDVQQGGQCGGLLSLCGPANPKFSLKENSSLGKDSNVTMEIPPHTTIAYTVIELEVKHDGRYDLCLTSDTTGGFEVDGSAKALLCTGDAPSQASENTLLTQELEKLSHYFQLLSDLPASTKSSLLQQITKVIVDRAAITSLENMLHQMCLNESPAKGEVKAESHNQDIQALLEALVLSGDAGQSASTLKALHLIVSALDEMTNTCLTLLGMCCSPTVLKTLELLVQHVSGSRELPQSGAGLAALTGDVYEKTQHLFASSNLSLKRDGDTLRTEIEHQAGNLPLVLCIAIRGLAALVHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZA32</accession>
    <name>A0A672ZA32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">mRNA export factor GLE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">GLE1 RNA export mediator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">GLE1-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Nucleoporin GLE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">gle1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GLE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000246.1">
        <property type="nucleotide sequence ID" value="XM_030144386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZA32"/>
    <dbReference type="FunCoup" id="A0A672ZA32">
        <property type="interactions" value="1354"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005013767.1">
        <property type="protein sequence ID" value="ENSSORP00005013357.1"/>
        <property type="gene ID" value="ENSSORG00005006336.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426337"/>
    <dbReference type="CTD" id="2733"/>
    <dbReference type="InParanoid" id="A0A672ZA32"/>
    <dbReference type="OrthoDB" id="420884at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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            <fullName evidence="20">Stromal interaction molecule 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">stim1a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZDB3"/>
    <dbReference type="Ensembl" id="ENSSORT00005015827.1">
        <property type="protein sequence ID" value="ENSSORP00005015336.1"/>
        <property type="gene ID" value="ENSSORG00005005862.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZDB3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
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    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <location>
            <begin position="1"/>
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        </source>
    </evidence>
    <sequence length="799" mass="89074" checksum="F71D14871C8066FD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
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        <location>
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            <end position="1587"/>
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            <begin position="159"/>
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    <feature type="domain" description="Spondin-like TSP1" evidence="17">
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            <begin position="319"/>
            <end position="372"/>
        </location>
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            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="730"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="995"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin-like TSP1" evidence="17">
        <location>
            <begin position="1243"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="domain" description="Thrombospondin type-1" evidence="18">
        <location>
            <begin position="1475"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="241"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF19028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1615" mass="179616" checksum="D6CE324BF2B602EC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZHJ4</accession>
    <name>A0A672ZHJ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Neurogenic mastermind-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">maml1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mastermind family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030002097.1">
        <property type="nucleotide sequence ID" value="XM_030146237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZHJ4"/>
    <dbReference type="Ensembl" id="ENSSORT00005016731.1">
        <property type="protein sequence ID" value="ENSSORP00005016224.1"/>
        <property type="gene ID" value="ENSSORG00005008226.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427625"/>
    <dbReference type="CTD" id="9794"/>
    <dbReference type="InParanoid" id="A0A672ZHJ4"/>
    <dbReference type="OrthoDB" id="5982619at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007221">
        <property type="term" value="P:positive regulation of transcription of Notch receptor target"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046369">
        <property type="entry name" value="MAML1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048455">
        <property type="entry name" value="MAML1_3_TAD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046370">
        <property type="entry name" value="MAML_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019082">
        <property type="entry name" value="Mastermind-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15692">
        <property type="entry name" value="MASTERMIND-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15692:SF19">
        <property type="entry name" value="MASTERMIND-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09596">
        <property type="entry name" value="MamL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20801">
        <property type="entry name" value="MAML1_3_TAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01275">
        <property type="entry name" value="MamL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Neurogenic mastermind-like N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="58"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="126"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="253"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="666"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="346"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="378"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="500"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="606"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="689"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1022" mass="108469" checksum="D7A37B6B99237551" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZIQ2</accession>
    <name>A0A672ZIQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZIQ2"/>
    <dbReference type="Ensembl" id="ENSSORT00005016875.1">
        <property type="protein sequence ID" value="ENSSORP00005016367.1"/>
        <property type="gene ID" value="ENSSORG00005008303.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZIQ2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000004">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="14">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="142" mass="15257" checksum="465D7DDE5C3596B2" modified="2020-06-17" version="1">MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAQRVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTEKAASSKKKKKKKTSSGSYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJU8</accession>
    <name>A0A672ZJU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Zinc finger protein-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115433146</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Required for cis-Golgi integrity and efficient ER to Golgi transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus</location>
            <location evidence="1 11">cis-Golgi network membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The B box-type and RING-type zinc fingers although degenerate play a central role in function of the protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ZFPL1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZJU8"/>
    <dbReference type="FunCoup" id="A0A672ZJU8">
        <property type="interactions" value="2491"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005017270.1">
        <property type="protein sequence ID" value="ENSSORP00005016757.1"/>
        <property type="gene ID" value="ENSSORG00005008474.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJU8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16487">
        <property type="entry name" value="mRING-H2-C3DHC3_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058730">
        <property type="entry name" value="U-box_ZFPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039043">
        <property type="entry name" value="ZFPL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058731">
        <property type="entry name" value="Znf-B_box_ZFPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12981">
        <property type="entry name" value="ZINC FINGER PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12981:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25998">
        <property type="entry name" value="U-box_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25993">
        <property type="entry name" value="zf-B_box_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="54"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="35073" checksum="7077DAF055B8191A" modified="2020-06-17" version="1">MGLCKCPKRKVTNLFCFEHRVNVCEHCLSPTTTRFCIVQSYLQWLQDSDYNPNCALCDHPLNAQDTVRLVCYDVFHWSCLNDLSSRLPVHTAPAGYQCPSCQGPLFPPPNLASPIADVLREQLSSVNWARAGLGLPLMEEPMGVPEEVGPADVTDYNDWSMFDGESLNMDVVMEGAPAQDDVGGVGPNGASDLQDHTVVNFTTPNTSDTLTIHTASSPRKVYDTRDAARSSATHIDFDDDKYRRRPALGWFAQILKNRTGPKRASLSWKQRVFMLLLVGVLGFFTLIIIMAKLGRASAGSDPNLDPLLNPNIRVGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZL33</accession>
    <name>A0A672ZL33_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Growth differentiation factor 6a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZL33"/>
    <dbReference type="FunCoup" id="A0A672ZL33">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018417.1">
        <property type="protein sequence ID" value="ENSSORP00005017891.1"/>
        <property type="gene ID" value="ENSSORG00005008896.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZL33"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035239">
        <property type="term" value="P:tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13766">
        <property type="entry name" value="TGF_beta_GDF5_6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000027">
        <property type="entry name" value="Growth differentiation factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF43">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="297"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="276"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="420" mass="48197" checksum="22CD847EF1EE12A8" modified="2020-06-17" version="1">LQLKVDSKMCVCDMELFGKPCNERPSRIPHQDGQRSTKFLKDIFASSHPIMSHHKDDPKDAIVPHEYMLSIYRTYSAAEKLGLNASFFRSSKSANTITSFVDRGTGWFQNEPQQKYLFDVSTLSDKEELVGAELRIFRRAPGDLQTSLQTTGLYDIQLYPCRSDRLLDSRSLDPLDSTKPGWEVLDLWDMFKTHHHHQQHQNQLCFQLRVTLGKSDTEVDLRRLGLDRRGRSQQEKAILVAYTRSKKRENLFNEMKEKIKSRRSVGEKEEAAVVVKAAEEEAEEQAPRGGRGEGPRRRRRTALSNRHGKRHGKKSKSRCSKKALHVNFKELGWDDWIIAPLDYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPNSTPPSCCVPTKLSPISILYIDSGNNVVYKQYEDMVVEQCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZNB0</accession>
    <name>A0A672ZNB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 74</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZNB0"/>
    <dbReference type="FunCoup" id="A0A672ZNB0">
        <property type="interactions" value="705"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005018424.1">
        <property type="protein sequence ID" value="ENSSORP00005017898.1"/>
        <property type="gene ID" value="ENSSORG00005008900.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZNB0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029695">
        <property type="entry name" value="TMEM74-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16125">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16125:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14927">
        <property type="entry name" value="Neurensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="220" mass="23969" checksum="68D370981416A558" modified="2020-06-17" version="1">PNADTVTGVKGAHLSDDKVRICCDEELETSFTYIDENVNLRVSSPETSCKSAQRTVRNGEPCSETLPELSFMSEDDLSFGEGPGTSIDYGFISAVTFLVTGISLVIISYAVPRDVVVDRDSVSAREMERLEMESARIGAHLDRCVIAGLCLLTLGGVVLSTLLMISMWKGEMYRRKVIAYSKRSAKLYGSISLKTRSSPSPSPSSAHLSADEEELEETLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZNT6</accession>
    <name>A0A672ZNT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZNT6"/>
    <dbReference type="Ensembl" id="ENSSORT00005019372.1">
        <property type="protein sequence ID" value="ENSSORP00005018821.1"/>
        <property type="gene ID" value="ENSSORG00005009261.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZNT6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="3"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="267" mass="29898" checksum="F8F26001C65CE48D" modified="2020-06-17" version="1">SVMRLTASPSSRIVHLGEPAIFTCVLPPNEISVTEVHWYKQTVGKNLKLIVKLKKNIEPTYGPQFSESRVFVHIDKNYSNLTILRTTPEDEGLYHCGVLDYITNTWSGTYLSLTNYTVVQRPTVSDPLHPGDSMSLQCSVLSDSDNKTCPGDHSVYWVRVGSHESDPGIIYTEGNRQDGCNDRPDTQSSPKSCLHHFSKTVNSSDAGTYYYYCAVATCGRILFGNGTRIQIGMFLLRSERGNTVIFYNIDSPWMETLGLCSVNDRFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZXK1</accession>
    <name>A0A672ZXK1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gadd45b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GADD45 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029987566.1">
        <property type="nucleotide sequence ID" value="XM_030131706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZXK1"/>
    <dbReference type="FunCoup" id="A0A672ZXK1">
        <property type="interactions" value="675"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005021590.1">
        <property type="protein sequence ID" value="ENSSORP00005020963.1"/>
        <property type="gene ID" value="ENSSORG00005010237.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115417679"/>
    <dbReference type="InParanoid" id="A0A672ZXK1"/>
    <dbReference type="OrthoDB" id="5976967at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000012">
        <property type="entry name" value="growth arrest and DNA damage-inducible protein GADD45 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024824">
        <property type="entry name" value="GADD45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411:SF5">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="2">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="160" mass="17843" checksum="BB813ADAF1075B65" modified="2020-06-17" version="1">MTLEELVGSNTTDKKMETVAQALEDLLVAAQCQDCLTVGVYESAKLMNVDPDSVVLCLLATDEEDADDIALQIHFTLLQAFCCDNDINIVRVSGMRRLAQLLEEDTEDSNGNEPRDLHCILVTNPPVQPLQCQALQDVSSFCEESRCRNQWVPSLELQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY93</accession>
    <name>A0A672ZY93_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZY93"/>
    <dbReference type="Ensembl" id="ENSSORT00005022093.1">
        <property type="protein sequence ID" value="ENSSORP00005021447.1"/>
        <property type="gene ID" value="ENSSORG00005010492.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZY93"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="45"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="299" mass="33788" checksum="810D8B3D932A5B76" modified="2020-06-17" version="1">METEDSELCFPELINSSCVKGVVNSLVTTLLIVLEFITALSTAFLNLLVIISVSHFRQLLSSSNFILLSLAVSEFFIYLMIYPFEVLYPYTCWFWGDLLCFLKSIFSVMIVSASVGTMVLISVDRYVAVCDPLHYSTRMSVKVVSVGLSLCWSSSFHFSLVFLDKNMEDLNFPRPCYGKCFYRVTFPLVLFELILTFFLPVVIVVLYMRVFVVVLSQVRAMHSHISAITFGPSATGNAKKSEVKAAKNLGVAVVVFLICFIPYYILILPWNLSKDSSFLVFLGRLLLLNSCFNPFLYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY97</accession>
    <name>A0A672ZY97_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZY97"/>
    <dbReference type="Ensembl" id="ENSSORT00005022098.1">
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        <property type="gene ID" value="ENSSORG00005010496.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZY97"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00408">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="54"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="164" mass="18387" checksum="5B14DFDAFD06095E" modified="2025-10-08" version="2">MQQNPLPNLQQLLQENLVGLKQFLNLSNLKERVYLYGRGSLGLGTLQEKVQGIPPAFLKPLIKKRVFENDTLKFYAEVFGLPSPEVKWFCNKTQLVAGDRVQMERDGDSISLIIRNVTKADQGEYICEAVNYVGEARSVALVVVVSQEVEIPCKTHLNLSINGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZYI6</accession>
    <name>A0A672ZYI6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ketohexokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">khk</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="ketohexokinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42774:SF3">
        <property type="entry name" value="KETOHEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42774">
        <property type="entry name" value="PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025581299" evidence="1">
        <location>
            <begin position="23"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="2">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="228" mass="25533" checksum="5F19E3B42B56BFB2" modified="2020-06-17" version="1">MGQSKILCVGLVCLDIINVVDKYPEEDTDSRCLSQRWQRGGNASNSCTVLSLLGASTAFMGSLAAGPVADFILEDFQKFHIDVSLVSEHAQCSLPASMVISNVSTGSRTILHMNSPHHRDGPQPRPRTAPPIRKQYCMCVCVCFLQFHSERLFAAFDRYFSCGLAGQRSDSVCMLCGLSIQRLSNRRPLRHVRCSRGRSHTTVIKQLCYHGDQSHIYSKPRHVFIFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A375</accession>
    <name>A0A673A375_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC115423194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A375"/>
    <dbReference type="Ensembl" id="ENSSORT00005024455.1">
        <property type="protein sequence ID" value="ENSSORP00005023756.1"/>
        <property type="gene ID" value="ENSSORG00005011482.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A375"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="191" mass="20062" checksum="D82EC2538BA4B9FC" modified="2020-06-17" version="1">MAVVRVSDGYGEVSAKFIYSEQDEEEVEYEDYSVQYHYKGRNARAGVLRASAATNDAYVGFDVLCADEDSFDEDEFDKNSGRRRRKKGGRVRAGVLSATAACDGFAAQVKGPNISAGVEESDTGVKVYAGAELASASASAGPVKAKVGLSAETGVGVGKDGVEAKVLGTGFSLGRKFGISVFGSGFEIDLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A4H9</accession>
    <name>A0A673A4H9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GREB1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">greb1l</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GREB1 family.</text>
    </comment>
    <dbReference type="FunCoup" id="A0A673A4H9">
        <property type="interactions" value="757"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024218.1">
        <property type="protein sequence ID" value="ENSSORP00005023537.1"/>
        <property type="gene ID" value="ENSSORG00005011326.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A4H9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001822">
        <property type="term" value="P:kidney development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028422">
        <property type="entry name" value="GREB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048659">
        <property type="entry name" value="GREB1-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046927">
        <property type="entry name" value="GREB1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048657">
        <property type="entry name" value="GREB1-like_cpSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046926">
        <property type="entry name" value="GREB1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049100">
        <property type="entry name" value="TAGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15720:SF12">
        <property type="entry name" value="GREB1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15720">
        <property type="entry name" value="GREB1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20692">
        <property type="entry name" value="cpSF2-GREB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20688">
        <property type="entry name" value="GREB1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20267">
        <property type="entry name" value="GREB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15782">
        <property type="entry name" value="GREB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20691">
        <property type="entry name" value="TAGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GREB1 N-terminal" evidence="7">
        <location>
            <begin position="78"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like second" evidence="9">
        <location>
            <begin position="332"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like circularly permuted SF2 helicase" evidence="11">
        <location>
            <begin position="660"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="domain" description="TET-Associated Glycosyltransferase" evidence="10">
        <location>
            <begin position="1502"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like C-terminal" evidence="8">
        <location>
            <begin position="1735"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1166"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1107"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1139"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1166"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1183"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1898" mass="210973" checksum="C9E48A9CB373D00C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5Q6</accession>
    <name>A0A673A5Q6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PAK4-inhibitor INKA2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">PAK4-inhibitor inka2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">inka2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Inhibitor of the serine/threonine-protein kinase PAK4. Acts by binding PAK4 in a substrate-like manner, inhibiting the protein kinase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with PAK4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the INKA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029990478.1">
        <property type="nucleotide sequence ID" value="XM_030134618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A5Q6"/>
    <dbReference type="FunCoup" id="A0A673A5Q6">
        <property type="interactions" value="1175"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005025286.1">
        <property type="protein sequence ID" value="ENSSORP00005024569.1"/>
        <property type="gene ID" value="ENSSORG00005011818.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419680"/>
    <dbReference type="CTD" id="55924"/>
    <dbReference type="InParanoid" id="A0A673A5Q6"/>
    <dbReference type="OrthoDB" id="9931119at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000319">
        <property type="entry name" value="Inka box actin regulator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029267">
        <property type="entry name" value="FAM212"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039201">
        <property type="entry name" value="Inka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28615">
        <property type="entry name" value="PAK4-INHIBITOR INKA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28615:SF2">
        <property type="entry name" value="PAK4-INHIBITOR INKA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15342">
        <property type="entry name" value="FAM212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM212" evidence="9">
        <location>
            <begin position="228"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="64"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="271"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="439" mass="48940" checksum="F7B2749A79BAD8EC" modified="2020-06-17" version="1">MEQQLSKPECKNMDACLRRLKQELLSMKEAGDGLHAQMDSMMGALQELKLLQVQTALENLDISGRPINRGLPHPAPQAPPNVPTVTASASGKDLGTCFSHDMLEEPKPSPVPSPRPSLGSRNTISGDERSLSQNRSSLGTSSTSSSSLESESESGRSARSENDLESIPRRWSGYTAPQVDFYGPVVGNPPPEPYPHPQPPCRAQAVDLPGILYSLSREGPSLDSEYSQESADDGSDWTSSLMSRSRNRQPLVLGDNVFADLVGNWLDLPEVEREEGEEEERKKRREERITDGGADRPDTPAHPLRLSRSQEICRKFSLTTNIFKKFLRSVRPDRDKLLKERPGWMAPELPEGDLSKRPKKVAAKGSKGSFYLPFWANGQQGKGRPCPHLAEAERNHHPQHHLPQIHHQPFAGIYLDRRQLQTGLEKMQPLFDYNTAVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A603</accession>
    <name>A0A673A603_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Leucine rich repeat containing G protein-coupled receptor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">lgr5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A603"/>
    <dbReference type="Ensembl" id="ENSSORT00005025398.1">
        <property type="protein sequence ID" value="ENSSORP00005024669.1"/>
        <property type="gene ID" value="ENSSORG00005011873.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A603"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000732">
        <property type="entry name" value="GD11101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF71">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="406"/>
            <end position="431"/>
        </location>
    </feature>
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        <location>
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            <end position="464"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="484"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="540"/>
        </location>
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        <location>
            <begin position="560"/>
            <end position="585"/>
        </location>
    </feature>
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            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
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        <location>
            <begin position="422"/>
            <end position="658"/>
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            <begin position="705"/>
            <end position="733"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="733" mass="80255" checksum="B309C580B87E292B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABQ8</accession>
    <name>A0A673ABQ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SI:CH73-42K18.1-RELATED"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
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        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="436" mass="50499" checksum="EDAF4BA68E6ACEB7" modified="2020-06-17" version="1">MAVQQKMTMRFIFSEGDIRKITLTTQPETLQDLINLLRSTLQTTYDFSLQYQDPEFNNELCNLTHPSELPGKPTLKIVPTIELVPVPPQDDSDTFSQWPEDFEIPKFPVDVEYRLRQGNLIYMRDSTYLKVTKELKHEILEKLAEVMYVFKAYPTKDEFNAVAKALVQTHPCLKEPGSITDWDGWRNSLKFKMGNYRSKMRLPGRLDVTVNSRKSGRFPTEDDPHNKETKKPRKSEINFLPNYPEGLDQHHLEEARQVLVQEMKKAKPNGSLVKKEMNMTFAQRRAEVVKDKPPIIEMVQRWPALFTESQVFLELTRVVGRNLKEDFFGELDRSSPCLMDLFRKNKGLTGQILAELLWKMKTTEPTDLRCLCIRGLPVIFGDDSAAFFKLWLNPTSSSRHPDLLNLLVACRNQCLARLFQCIVKIYVYSMAITLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABX3</accession>
    <name>A0A673ABX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial glutathione transporter SLC25A40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 25 member 40</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">slc25a40</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>glutathione(in) = glutathione(out)</text>
            <dbReference type="Rhea" id="RHEA:74819"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030014392.1">
        <property type="nucleotide sequence ID" value="XM_030158532.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030014393.1">
        <property type="nucleotide sequence ID" value="XM_030158533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ABX3"/>
    <dbReference type="FunCoup" id="A0A673ABX3">
        <property type="interactions" value="1395"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026801.1">
        <property type="protein sequence ID" value="ENSSORP00005026032.1"/>
        <property type="gene ID" value="ENSSORG00005012509.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435906"/>
    <dbReference type="CTD" id="55972"/>
    <dbReference type="InParanoid" id="A0A673ABX3"/>
    <dbReference type="OrthoDB" id="1747031at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF5">
        <property type="entry name" value="MITOCHONDRIAL GLUTATHIONE TRANSPORTER SLC25A40-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="14"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="140"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="234"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="345" mass="38163" checksum="95358DFDE3090EAE" modified="2020-06-17" version="1">MSGQSRASPASDGITPLQQMVASCSGALLTSLFVTPLDVVKIRLQAQMNPFPKGKCFVYCNGLMDHICVCDNGNSKAWYKAPGHFSGTLDAFVKIIRREGIKSLWSGLPPTLVMAVPATVIYFTCYDQLCAVLRVKMGDRASEAPLLAGAIARVGSATVISPLELIRTKMQSQKLTYRELTDCIRSAVEAEGRLSMWRGLGPTLLRDVPFSAMYWYNYEKGKAWLCERYNTREPTFAITFVSGASSGSFAAIVTLPFDVVKTRRQVELGELQARNLSHQVSSSTFSVMKRIVMQDGFSGLFAGFLPRLIKVAPACAIMISTYEFGKAFFRKHNQERIHRPLQTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACN1</accession>
    <name>A0A673ACN1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Nectin-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115436608</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030015357.1">
        <property type="nucleotide sequence ID" value="XM_030159497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ACN1"/>
    <dbReference type="Ensembl" id="ENSSORT00005027039.1">
        <property type="protein sequence ID" value="ENSSORP00005026267.1"/>
        <property type="gene ID" value="ENSSORG00005012596.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436608"/>
    <dbReference type="CTD" id="794920"/>
    <dbReference type="InParanoid" id="A0A673ACN1"/>
    <dbReference type="OrthoDB" id="8872282at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF11">
        <property type="entry name" value="NECTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025594300" evidence="14">
        <location>
            <begin position="27"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="34"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="146"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="239"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="504"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="540"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="596" mass="66444" checksum="69AB3584B190B138" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEF0</accession>
    <name>A0A673AEF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC115410333</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Early endosome membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_029977798.1">
        <property type="nucleotide sequence ID" value="XM_030121938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AEF0"/>
    <dbReference type="Ensembl" id="ENSSORT00005028450.1">
        <property type="protein sequence ID" value="ENSSORP00005027656.1"/>
        <property type="gene ID" value="ENSSORG00005013203.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410333"/>
    <dbReference type="InParanoid" id="A0A673AEF0"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000180">
        <property type="entry name" value="Member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="219" mass="23513" checksum="13C728417CF3A605" modified="2020-06-17" version="1">MAARGGGASRPNGTAAANKICQFKLVLLGESAVGKSSLVLRFAKGQFQDNLESTIGAAFVTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNTDTFARAKNWVKELQRQASTNIVIALAGNKADIANKRAVELKEAQTYADDNSLLFMETSAKTAMNVNEIFMAIAKKLPKNDPQGAGQGGRTRTGVDLQEAAPQGRSSHCCGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEQ1</accession>
    <name>A0A673AEQ1_9TELE</name>
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        <recommendedName>
            <fullName evidence="17">Voltage-dependent L-type calcium channel subunit alpha</fullName>
        </recommendedName>
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        <name evidence="21" type="primary">cacna1fb</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005027233.1"/>
        <property type="gene ID" value="ENSSORG00005012973.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AEQ1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
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    </dbReference>
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        <property type="entry name" value="VDCCAlpha1"/>
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        <property type="entry name" value="Volt_channel_dom_sf"/>
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    <dbReference type="Pfam" id="PF00520">
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    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01630">
        <property type="entry name" value="LVDCCALPHA1"/>
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    <dbReference type="SMART" id="SM01062">
        <property type="entry name" value="Ca_chan_IQ"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 17" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3 17" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9 17" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
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    <feature type="transmembrane region" description="Helical" evidence="19">
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    <feature type="transmembrane region" description="Helical" evidence="19">
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    <feature type="transmembrane region" description="Helical" evidence="19">
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            <begin position="283"/>
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    <feature type="transmembrane region" description="Helical" evidence="19">
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            <begin position="316"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="19">
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            <begin position="470"/>
            <end position="488"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
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    <feature type="transmembrane region" description="Helical" evidence="19">
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            <begin position="600"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="689"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="760"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="791"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
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            <begin position="833"/>
            <end position="856"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1001"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
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            <begin position="1080"/>
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    <feature type="transmembrane region" description="Helical" evidence="19">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
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            <end position="1225"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1298"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="domain" description="Voltage-dependent calcium channel alpha-1 subunit IQ" evidence="20">
        <location>
            <begin position="1456"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
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        <location>
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        <ligand>
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        <ligand>
            <name>Ca(2+)</name>
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        <location>
            <position position="262"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1518" mass="173421" checksum="6C8D2A6089CBF224" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AG59</accession>
    <name>A0A673AG59_9TELE</name>
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        <submittedName>
            <fullName evidence="12">Capping protein regulator and myosin 1 linker 3</fullName>
        </submittedName>
    </protein>
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        <name evidence="12" type="primary">carmil3</name>
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    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CARMIL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AG59"/>
    <dbReference type="FunCoup" id="A0A673AG59">
        <property type="interactions" value="532"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005029037.1">
        <property type="protein sequence ID" value="ENSSORP00005028234.1"/>
        <property type="gene ID" value="ENSSORG00005013502.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AG59"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000009">
        <property type="entry name" value="F-actin-uncapping protein LRRC16A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031943">
        <property type="entry name" value="CARMIL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041245">
        <property type="entry name" value="CARMIL_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF43">
        <property type="entry name" value="CAPPING PROTEIN, ARP2_3 AND MYOSIN-I LINKER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17888">
        <property type="entry name" value="Carm_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16000">
        <property type="entry name" value="CARMIL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CARMIL pleckstrin homology" evidence="11">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="CARMIL C-terminal" evidence="10">
        <location>
            <begin position="738"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="861"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="912"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1027"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="880"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="916"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1069"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1094"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1114"/>
            <end position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1242" mass="137635" checksum="46D1D21D9D7F09AC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AG60</accession>
    <name>A0A673AG60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase FANCL</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase FANCL</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">fancl</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673AG60"/>
    <dbReference type="Ensembl" id="ENSSORT00005028509.1">
        <property type="protein sequence ID" value="ENSSORP00005027713.1"/>
        <property type="gene ID" value="ENSSORG00005013250.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AG60"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23832">
        <property type="entry name" value="DRWD-C_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23831">
        <property type="entry name" value="DRWD-N_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16490">
        <property type="entry name" value="RING-CH-C4HC3_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000081">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.20:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000221">
        <property type="entry name" value="E3 ubiquitin-protein ligase FANCL isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.20">
        <property type="entry name" value="RWD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026848">
        <property type="entry name" value="Fancl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026850">
        <property type="entry name" value="FANCL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043898">
        <property type="entry name" value="FANCL_d2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044037">
        <property type="entry name" value="FANCL_d3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043003">
        <property type="entry name" value="FANCL_d3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN PHF9 FANCONI ANEMIA GROUP L PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11793">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18890">
        <property type="entry name" value="FANCL_d2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18891">
        <property type="entry name" value="FANCL_d3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="233"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="295" mass="33802" checksum="7593FBF7B10EEB85" modified="2020-06-17" version="1">RGYEHIVKQVNHTCTLLLEVGLKSRPECRSIPPPQYYSQLISEMETLGWDKLLFIDTEFQTLRLKAEDSSGRQHILTVKLKPKHPAEAPECSADLPLPLDFTWTPQSTLDQLHSQFLLVLESLSDFWNILDEIDSQTWILEPEKPSRSDTTRRIAIGNNVSIKVEVDPRHPKMLPECCLLGAEHVVTPLRNKLNANMHMWNPDSSVLHNLRDVLEIEFPSPATHEKSSFSVECGICYSYRLEAAIPDQVCNDPRCGQPFHQACLYEWLRALPSSRQSFNIVFGECPYCSKVRHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AKZ7</accession>
    <name>A0A673AKZ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Interleukin-1 receptor type 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115411828</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029979895.1">
        <property type="nucleotide sequence ID" value="XM_030124035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AKZ7"/>
    <dbReference type="FunCoup" id="A0A673AKZ7">
        <property type="interactions" value="162"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030873.1">
        <property type="protein sequence ID" value="ENSSORP00005030031.1"/>
        <property type="gene ID" value="ENSSORG00005014343.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411828"/>
    <dbReference type="InParanoid" id="A0A673AKZ7"/>
    <dbReference type="OrthoDB" id="9940746at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF26">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025682461" evidence="10">
        <location>
            <begin position="25"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="109"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="215"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="11">
        <location>
            <begin position="373"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="556" mass="63241" checksum="D2AEE53EBD7496F5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQS1</accession>
    <name>A0A673AQS1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">klhl24a</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AQS1"/>
    <dbReference type="Ensembl" id="ENSSORT00005031619.1">
        <property type="protein sequence ID" value="ENSSORP00005030757.1"/>
        <property type="gene ID" value="ENSSORG00005014666.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQS1"/>
    <dbReference type="OrthoDB" id="19132at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="CDD" id="cd18253">
        <property type="entry name" value="BTB_POZ_KLHL24_KRIP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030596">
        <property type="entry name" value="BTB_POZ_KLHL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF215">
        <property type="entry name" value="KELCH-LIKE PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="66"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="600" mass="68009" checksum="548FF2E1FC776CBF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AS90</accession>
    <name>A0A673AS90_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Protein tyrosine phosphatase receptor type N2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AS90"/>
    <dbReference type="FunCoup" id="A0A673AS90">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033314.1">
        <property type="protein sequence ID" value="ENSSORP00005032426.1"/>
        <property type="gene ID" value="ENSSORG00005015375.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AS90"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035773">
        <property type="term" value="P:insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051046">
        <property type="term" value="P:regulation of secretion"/>
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        <property type="entry name" value="PRTYPHPHTASE"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
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            <begin position="390"/>
            <end position="414"/>
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            <begin position="523"/>
            <end position="783"/>
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            <begin position="702"/>
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            <begin position="111"/>
            <end position="131"/>
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            <begin position="455"/>
            <end position="497"/>
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            <end position="165"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="ARBA" id="ARBA00023329"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032426.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="793" mass="88002" checksum="108C473774250B95" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AW37</accession>
    <name>A0A673AW37_9TELE</name>
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        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">avpr1aa</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.</text>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="V1R_C"/>
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        <property type="entry name" value="Vasoprsn_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Vprs_V1A_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VASOPRESSIN V1A RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="VASOPRESSINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00752">
        <property type="entry name" value="VASOPRSNV1AR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
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    </dbReference>
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        <property type="entry name" value="DUF1856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="75"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="146"/>
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        <location>
            <begin position="321"/>
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        </location>
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        <location>
            <begin position="67"/>
            <end position="341"/>
        </location>
    </feature>
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        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
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        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU046427"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="410" mass="46978" checksum="0418735A2795F921" modified="2020-06-17" version="1">MHTPDYALLLSAGNQSVDFSPTHDLRMETSENTTAHPNGSDPYARNEEVAKIEIMVLSITFVVAVMGNISVLLAMYNTKKKMSRMHLFIKHLSLADLVVAFFQVLPQLCWEITYRFYGPDFLCRIVKHLQVMGMFASTYMMVMMTVDRYIAICHPLKTLQQPTQRSYIMIISTWMCSLVLSTPQYFIFSLSEIKNGSDVYDCWAHFIEPWGAKAYITWITVGIFLVPVVILMICYGFICHSIWKNIQYKKRKTMPGAANKKGLIGKNSVSSITTISRAKLRTVKMTFVIVLAYIICWAPFFIVQMWSVWDENFQWDDSENTAVTLSALLASLNSCCNPWIYMIFSGHLLQDFVHCFSCCHKMNSNFRKEDSDSSLRRTTLLTKMTNRSPTASSVNWRELDNSPKSSIQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWB1</accession>
    <name>A0A673AWB1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Neuropilin (NRP) and tolloid (TLL)-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115436975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AWB1"/>
    <dbReference type="Ensembl" id="ENSSORT00005034853.1">
        <property type="protein sequence ID" value="ENSSORP00005033940.1"/>
        <property type="gene ID" value="ENSSORG00005016054.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AWB1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000463">
        <property type="term" value="P:positive regulation of excitatory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000016">
        <property type="entry name" value="neuropilin and tolloid-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF27">
        <property type="entry name" value="NEUROPILIN AND TOLLOID-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025371448" evidence="5">
        <location>
            <begin position="22"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="43"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="182"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="523" mass="58919" checksum="FA452E5CAD76CC05" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AWI8</accession>
    <name>A0A673AWI8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">macrod1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030000680.1">
        <property type="nucleotide sequence ID" value="XM_030144820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AWI8"/>
    <dbReference type="FunCoup" id="A0A673AWI8">
        <property type="interactions" value="603"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034961.1">
        <property type="protein sequence ID" value="ENSSORP00005034046.1"/>
        <property type="gene ID" value="ENSSORG00005016104.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426681"/>
    <dbReference type="CTD" id="28992"/>
    <dbReference type="InParanoid" id="A0A673AWI8"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140293">
        <property type="term" value="F:ADP-ribosylglutamate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042278">
        <property type="term" value="P:purine nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02908">
        <property type="entry name" value="Macro_OAADPr_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001664">
        <property type="entry name" value="PRK00431.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF93">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE MACROD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="174"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="368" mass="39812" checksum="78FC2C279B312427" modified="2020-06-17" version="1">MALQISALFSHLRALPCIPGPLFRVQRTGVRFKYILCPGAPQPGRTPATGLIRGHINAEHRAKFSTTAAAAFWKSSPVSSPSTHRRRTGWLGRVALGAALGVSTAALVQCLHTGTLAAMARKVNLNSAEGDWKETKDFLLSLSVKDRCQYYRTSKSVPLDEIPLWSPTAGGSQQSRYPRDEKLDQKISLYSGDITMLEIDAIVNAANKTLLGGGGVDGAIHRAAGPLLKKECASLHGCETGQAKITCGYGLPAKFVIHTVGPIAQGGVGEDERKALRSCYRNSLKTATENAARSVAFPCISTGIYGYPPEQAVHEALATVRDYLDEHHDKLDRVIFCVFLPTDKELYLQNLPLYFPAAPTEATVKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AYY4</accession>
    <name>A0A673AYY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Solute carrier family 35 member G1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Transmembrane protein 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">slc35g1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May play a role in intracellular calcium sensing and homeostasis. May act as a negative regulator of plasma membrane calcium-transporting ATPases preventing calcium efflux from the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with STIM1; stimulated by depletion of intracellular calcium. Interacts with ORAI1. Interacts with the plasma membrane calcium-transporting ATPases ATP2B1 and ATP2B4. Interacts with ATP1A1, ATP2A2, KPNB1 and XPO1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TMEM20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030018993.1">
        <property type="nucleotide sequence ID" value="XM_030163133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AYY4"/>
    <dbReference type="FunCoup" id="A0A673AYY4">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005034501.1">
        <property type="protein sequence ID" value="ENSSORP00005033592.1"/>
        <property type="gene ID" value="ENSSORG00005015912.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439307"/>
    <dbReference type="CTD" id="159371"/>
    <dbReference type="InParanoid" id="A0A673AYY4"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990034">
        <property type="term" value="P:calcium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.20:FF:000026">
        <property type="entry name" value="Solute carrier family 35, member G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="16">
        <location>
            <begin position="106"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="16">
        <location>
            <begin position="259"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="26"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="403" mass="44856" checksum="1B3FE5625464A6A3" modified="2020-06-17" version="1">MGDCNHCAHERPLTAEDGVTVVFHKVDSRERNTDDDRDVEGTAERVHLHRNSNVSSGWNNDDEDDDDAEETTRKHRVDSRLCPPASCVTATSARKGSDKPKRCPGLGLFYAFLSTVFFSIIALLVKTIEGLHAIEISALRCFFQMLFVVPLLIYHKTGFLGPRDKRIYLVLRGFIGSNAMIMLFYAVQQMPLADATVIMFSNPVFTSLLAWIFLKERCTIWDCVFTVFTLTGVILIARPPFLFGKHLGGIEGNYTNHIKGTAAAFAGAIGAAFTFVVLRKIGKSAHYYLSVWYYAVIGLMECIITLSVLGEWKLPSCGRDRWLLMLIAVLGIAGQSFLTKALQIEKAGPVALMRTVDVVLAFIFQFIFFNRAPTWWSLGGALCVVASTSGVALRKWYSNSRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AZB1</accession>
    <name>A0A673AZB1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein phosphatase 1 regulatory subunit 3G</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AZB1"/>
    <dbReference type="Ensembl" id="ENSSORT00005035914.1">
        <property type="protein sequence ID" value="ENSSORP00005034985.1"/>
        <property type="gene ID" value="ENSSORG00005016494.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AZB1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF7">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 3G"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="146"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="270" mass="30243" checksum="C65EF66836AD1F36" modified="2020-06-17" version="1">MSRSPLLFRSGPPDPGPGLENGLDPDQDEDLDDEVDASRLERFMRDRRRAQSLPAYPAELLERVPEEEEGRKRVKFADALGLNLASVKHFSALEEPQIPTKVLSRHRSFPPPQDFLCHGLKSVPDTDRLVSCFPEPRDADRRVRDLRVCLEKVSVTQFDVRGQIRALSGGGEAGVREVGVRYTFNEWLSHVDAQALPVSAEPPPGLVGQRFAFTVYTPPVMDPSSAVHFAVFLRSDDGEFWDNNQGHNYTLRYRCMPGNEGFVSAAFHAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0L9</accession>
    <name>A0A673B0L9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ALK and LTK ligand 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ALKAL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B0L9"/>
    <dbReference type="Ensembl" id="ENSSORT00005035862.1">
        <property type="protein sequence ID" value="ENSSORP00005034934.1"/>
        <property type="gene ID" value="ENSSORG00005016460.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B0L9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030298">
        <property type="term" value="F:receptor signaling protein tyrosine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070378">
        <property type="term" value="P:positive regulation of ERK5 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029364">
        <property type="entry name" value="ALKL1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28676:SF2">
        <property type="entry name" value="ALK AND LTK LIGAND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28676">
        <property type="entry name" value="ALK AND LTK LIGAND 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15129">
        <property type="entry name" value="ALKL1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005034934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="53" mass="6156" checksum="AF3ED722F59C066E" modified="2020-06-17" version="1">MSSLLGPLYFSHKCRKHAYRLYHHTRDCTIPAYFKRCARLLTRLAGSPQCTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B3N4</accession>
    <name>A0A673B3N4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Orofacial cleft 1 candidate gene 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ofcc1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673B3N4"/>
    <dbReference type="Ensembl" id="ENSSORT00005037366.1">
        <property type="protein sequence ID" value="ENSSORP00005036405.1"/>
        <property type="gene ID" value="ENSSORG00005017155.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B3N4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031390">
        <property type="entry name" value="OFCC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33862">
        <property type="entry name" value="OROFACIAL CLEFT 1 CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33862:SF3">
        <property type="entry name" value="OROFACIAL CLEFT 1 CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15680">
        <property type="entry name" value="OFCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="726"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="784"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="827"/>
            <end position="847"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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            <end position="93"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="131" mass="14910" checksum="F33B86A8E8859109" modified="2020-06-17" version="1">ISPRWCNSDRKSNSDRKCNSDRKSNSDRKCNSDRKKSNSDRKSNSDRKKSNSDRKCNSDRKSNSDRKSNSDRKSNSDRKCNSDRKSNSDRKSNSDVNVTEVICGVLKSSCSPTPEGPWMFSGLFLSVLMWS</sequence>
</entry>
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    <accession>A0A673B5D6</accession>
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        <submittedName>
            <fullName evidence="8">Connector enhancer of kinase suppressor of ras 3-like</fullName>
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        <name evidence="8" type="primary">cnksr3</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
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            <taxon>Apogoninae</taxon>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </location>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036549.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="968" mass="108126" checksum="EDA67185F65AE885" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B630</accession>
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        <recommendedName>
            <fullName evidence="15">Transformer-2 protein homolog beta</fullName>
        </recommendedName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Transformer-2 protein homolog B</fullName>
        </alternativeName>
    </protein>
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    </gene>
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        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.</text>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12363">
        <property type="entry name" value="RRM_TRA2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transformer-2 protein homolog beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 18" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="20">
        <location>
            <begin position="114"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="196"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="56"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="279" mass="32657" checksum="0A34DD50486BC679" modified="2020-06-17" version="1">MSDNERDFRKLDSRPGSRSASPRGSAKSANRSPSRSKDGSRHSRSRSQSRSESRSRSHRSSRRHYSRSRSHSHRRRSRSRSRSCEYRRRRSHSRSPMSNRRRHVGNRANPDPNTCLGVFGLSLYTTERDLRDVFSKYGPLTDVNIVYDQQSRRSRGFAFIYFENTEDSKEAKDRANGMELDGRRIRVDFSITKRAHTPTPGIYMGRPTYGGGGGGGGGGSSRRVSRDYDRGYDRGYDRDYNRYDDRDYYRSYRRRSPSPYYSRGYRSRSRSRSYSPRHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BA84</accession>
    <name>A0A673BA84_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BA84"/>
    <dbReference type="Ensembl" id="ENSSORT00005038342.1">
        <property type="protein sequence ID" value="ENSSORP00005037368.1"/>
        <property type="gene ID" value="ENSSORG00005017543.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BA84"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="2">
        <location>
            <begin position="28"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="122" mass="13508" checksum="7BB20D7064F4517C" modified="2020-06-17" version="1">SSGKLQLKKYVPAQPAEPVPSVSSARVPDEPNIPLPNKYSGNPETCRNFFTQIQLIFEAQPRRFSNDSAKIAYVASLLEGPPLSYFNALLEQESPVARSYSRLCAELKRVYDHPVRGWASGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BCL2</accession>
    <name>A0A673BCL2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BCL2"/>
    <dbReference type="Ensembl" id="ENSSORT00005041253.1">
        <property type="protein sequence ID" value="ENSSORP00005040216.1"/>
        <property type="gene ID" value="ENSSORG00005018760.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BCL2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="118" mass="14379" checksum="7F31FB8064B18FBA" modified="2020-06-17" version="1">RRKETPSRRKETPSRRKETPSRRKKTPWKETPSRRKETPSRRKETPSRRSETPSKRKETPSRRKETPSKRKETPSRRKETPSKRKETPSRRKETPSRWKETPSRTKETPSRREETMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BDB7</accession>
    <name>A0A673BDB7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Protein-tyrosine sulfotransferase</fullName>
            <ecNumber evidence="4 14">2.8.2.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tpst1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 14">Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16801"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:11688"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:65286"/>
            <dbReference type="EC" id="2.8.2.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the protein sulfotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.20" evidence="4 14"/>
    <dbReference type="AlphaFoldDB" id="A0A673BDB7"/>
    <dbReference type="FunCoup" id="A0A673BDB7">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041543.1">
        <property type="protein sequence ID" value="ENSSORP00005040501.1"/>
        <property type="gene ID" value="ENSSORG00005018900.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BDB7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008476">
        <property type="term" value="F:protein-tyrosine sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000290">
        <property type="entry name" value="Protein-tyrosine sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026634">
        <property type="entry name" value="TPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788:SF4">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="332"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="383" mass="42937" checksum="F679E8BC21FEE691" modified="2020-06-17" version="1">MIGKLKQNLLVACLVVSSVTVFYLGRHAMECHHRIEERSQPGGILPLSAIGGSMRTTLRTGQNLSTPFVYSKDMPLIFIGGVPRSGTTLMRAMLDAHPEVRCGEETRVIPRILAMKQMWSRSGREKMRLDEAGVTDEVLDAAMQAFLLEIIVKHGEPANFLCNKDPFALKSLSYLAKIFPRAKFVLMIRDGRASVHSMISRKVTIAGFDLGSYRDCLTKWNRAIETMYTQCLEVADKCLPVHYEQLVLHPEKWMRTLLKFLDIPWNDAVLHHEELIGKAGGVSLSKVERSTDQVIKPVNVEALSKWVGKIPADVVRDMAVIAPMLSRLGYDPHANPPNYGRPDPKVLDNTRRVSTKHTPKHPNPQIRSGMKKVHCCTSINDPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BF39</accession>
    <name>A0A673BF39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Abasic site processing protein HMCES</fullName>
            <shortName evidence="12">ES cell-specific 5hmC-binding protein</shortName>
            <ecNumber evidence="12">3.4.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 12">Peptidase HMCES</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">SRAP domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hmces</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. The HMCES DNA-protein cross-link is then either reversed or degraded. HMCES is able to catalyze the reversal of its thiazolidine cross-link and cycle between a cross-link and a non-cross-linked state depending on DNA context: mediates self-reversal of the thiazolidine cross-link in double stranded DNA, allowing APEX1 to initiate downstream repair of abasic sites. The HMCES DNA-protein cross-link can also be degraded by the SPRTN metalloprotease following unfolding by the BRIP1/FANCJ helicase. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_029989492.1">
        <property type="nucleotide sequence ID" value="XM_030133632.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029989494.1">
        <property type="nucleotide sequence ID" value="XM_030133634.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029989495.1">
        <property type="nucleotide sequence ID" value="XM_030133635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BF39"/>
    <dbReference type="Ensembl" id="ENSSORT00005039975.1">
        <property type="protein sequence ID" value="ENSSORP00005038973.1"/>
        <property type="gene ID" value="ENSSORG00005018226.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419026"/>
    <dbReference type="CTD" id="56941"/>
    <dbReference type="InParanoid" id="A0A673BF39"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="293"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="326"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="347" mass="39323" checksum="36917AD43C3EFD1D" modified="2020-06-17" version="1">MCGRTACTLAPDEVSRACSYRNRGGHRRQPGWRDGDADKYRPSYNKSPQSTSPVLLSQRHFDKTAPVDECVLAAMRWGLVPSWFKENDPSKMQYSTNNCRSESILQKKSYKDPLVKGQRCVILADGFYEWRRQEKNRQPFFIYFPQDQGAKSKDQSEPSTSQDNSEENSPNPSQEGESPCEWTGWKLLTMAGLFDCWTPPGGGEPLYTYTVITVNASPNLESIHDRMPAILDGEEEVRRWLDFGEVKSLDALKLLQSKNILTFHPVSSLVNNSRNNSAECLQPVDLNSKKELTPTASGRMMMSWLKSSGPTKRKEEEEKETTTNTQSKSTGPLQQWLQGANKKPRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFD0</accession>
    <name>A0A673BFD0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SKI/SNO/DAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BFD0"/>
    <dbReference type="Ensembl" id="ENSSORT00005040050.1">
        <property type="protein sequence ID" value="ENSSORP00005039048.1"/>
        <property type="gene ID" value="ENSSORG00005018249.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BFD0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="CDD" id="cd21082">
        <property type="entry name" value="DHD_SKIDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.20">
        <property type="entry name" value="Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052119">
        <property type="entry name" value="ElonginBC-PRC2_ViralRestrict"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027971">
        <property type="entry name" value="EPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003380">
        <property type="entry name" value="SKI/SNO/DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23187">
        <property type="entry name" value="FLJ44216 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23187:SF4">
        <property type="entry name" value="SKI_DACH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15223">
        <property type="entry name" value="EPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKI/SNO/DAC" evidence="2">
        <location>
            <begin position="6"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="459" mass="50943" checksum="DDCA9568E88EBD36" modified="2020-06-17" version="1">MGDLECGFEEMQGVRLGYLLIKGKQMFALSQVFTDLLKNIPRTTVHKRMDHLKVKKHHCDLEELRKLKAINSIAFHAAKCTLISREDVEALYISCKTERVLKSNKRKAKAVCSTEGEDASSGLLHADTELWKEKVWFSLHGVPETLTLHNKPGRRKELTPCLTDSKLPQFYHKSNGRDYRSAAKSSHKHFKNYETAKITGNPDTRTTTYHRCCSCKPKSSGTHGTSFGAFHLSPDFYLDPRPSPSSPASPIIIITPPTTSPLSRRATAATPSPAPTRTERTRTRTSARGLSTTSNSGSSEEEEEGEDEDDTQSESSEVSSDEEESSTQSDSSSVSSRVSVRASGSDGPGSVLWPNSEQHFEAPLVLQPTFHYNNPQQQDQPPRTLCHRSPAKMPFRLMANFPSPPSLVVGSDGDLCPAYSLNSLRGRGPPPPSPPVWRWQPGGQILPPPHAQRTRKYWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BG85</accession>
    <name>A0A673BG85_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-Q</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cyclin-related protein FAM58A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ccnq</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May be an activating cyclin for the cyclin-associated kinase CDK10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin-like FAM58 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993813.1">
        <property type="nucleotide sequence ID" value="XM_030137953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BG85"/>
    <dbReference type="FunCoup" id="A0A673BG85">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005041838.1">
        <property type="protein sequence ID" value="ENSSORP00005040785.1"/>
        <property type="gene ID" value="ENSSORG00005019037.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421941"/>
    <dbReference type="CTD" id="92002"/>
    <dbReference type="InParanoid" id="A0A673BG85"/>
    <dbReference type="OrthoDB" id="79090at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20534">
        <property type="entry name" value="CYCLIN_CCNM_CCNQ_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20535">
        <property type="entry name" value="CYCLIN_CCNM_CCNQ_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclin Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000042">
        <property type="entry name" value="FAM58A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048055">
        <property type="entry name" value="Cyclin-Q_first_cyclin_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048053">
        <property type="entry name" value="Cyclin-Q_second_cyclin_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028758">
        <property type="entry name" value="Cyclin, C/H/G types"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="44"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="257" mass="29306" checksum="FC17F8D41FD11119" modified="2020-06-17" version="1">MEGPSSRLSEARDGAVQPAGGRDASGEVESDSSRDVKTHFRVCRFIMETGVKLGMRSVPVATACVLYHRFFERVNISSYEPYLVAMSCVYLAGKVEEQHIRTRDIINVSHRYFNSGSAPLECDKEFWDLRDSVVQCELLILRQLNFHVSFEHPHKYLLHYLLSVKSLVNRHAWSRTPVAETSWALLRDCYHGAMCIRHAPQHIAIATLYLALNSYGVELPVGEKEWWQVLCEDVTRADIDAVISDLLQLYDMEAKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKF2</accession>
    <name>A0A673BKF2_9TELE</name>
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        <submittedName>
            <fullName evidence="6">PDZ domain containing 7a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115434123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BKF2"/>
    <dbReference type="Ensembl" id="ENSSORT00005043325.1">
        <property type="protein sequence ID" value="ENSSORP00005042250.1"/>
        <property type="gene ID" value="ENSSORG00005019583.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BKF2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060088">
        <property type="term" value="P:auditory receptor cell stereocilium organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10833">
        <property type="entry name" value="PDZ1_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10834">
        <property type="entry name" value="PDZ2_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
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    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051844">
        <property type="entry name" value="USH2_Complex_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116">
        <property type="entry name" value="PDZ DOMAIN CONTAINING WHIRLIN AND HARMONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116:SF29">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="74"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="198"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="873"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="379"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="456"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="504"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="719"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="719"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="768"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="789"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="840"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="975" mass="107482" checksum="AB28EAD13F5B501F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLC9</accession>
    <name>A0A673BLC9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin-conjugating enzyme E2 variant 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">uevld</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BLC9"/>
    <dbReference type="FunCoup" id="A0A673BLC9">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043116.1">
        <property type="protein sequence ID" value="ENSSORP00005042044.1"/>
        <property type="gene ID" value="ENSSORG00005019497.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLC9"/>
    <dbReference type="OrthoDB" id="5405561at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008883">
        <property type="entry name" value="UEV_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF33">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2 VARIANT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05743">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51322">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UEV" evidence="1">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="470" mass="51586" checksum="BD3431DED48BE273" modified="2020-06-17" version="1">MDLNSEKIQRILSKYKFHDVAIEELQKIHRIFPGMTPVPGTYTFSDSTQKDLLKLIGNIPVQYEGHTYNFPVQLWLMDSFPFTPPICLLRPTSNMVIREGKHVDTKGRIHLPALHNWDYPKSSVVGLLKEMIAKFEEDPPLSTKSTDDNKDPHKLLSFVSNFQINDGGIRQYNQPINKVSVIGGGDLGMASVMSIISKCDVDKLVFIDVAQSSTKGGSTDLEIFSLPKVEVSRDLSSSAGSRVVVVTANAWSSEQSYIRVVQTNVDMYRGIIPNLARLSPNAVMVIASQPADIMTHIAWRQSGLPPTQVIGAGCNLDSERLSHVLDINLNTHKQAWVIGELSDNKVPVMSNTGLNSSTHTEIAPGSTSTKPLLDRAFEMIKNRGQRSWSVGLSVADITNSILTNKMKIHSVSTLAQGWGGISAEVFLSLPCFLGSNGSTRLAGVPLGPEEDSKLRESVTSLCDLMTQLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLG9</accession>
    <name>A0A673BLG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA ligase</fullName>
            <ecNumber evidence="21">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">lig3</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="20">Isoform 3 interacts (via BRCT domain) with the nuclear DNA-repair protein XRCC1. Interacts with POLG. Interacts with POLB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="21"/>
    <dbReference type="RefSeq" id="XP_030009510.1">
        <property type="nucleotide sequence ID" value="XM_030153650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BLG9"/>
    <dbReference type="FunCoup" id="A0A673BLG9">
        <property type="interactions" value="1425"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005044624.1">
        <property type="protein sequence ID" value="ENSSORP00005043526.1"/>
        <property type="gene ID" value="ENSSORG00005017559.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432720"/>
    <dbReference type="CTD" id="3980"/>
    <dbReference type="InParanoid" id="A0A673BLG9"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070421">
        <property type="term" value="C:DNA ligase III-XRCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097681">
        <property type="term" value="P:double-strand break repair via alternative nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051053">
        <property type="term" value="P:negative regulation of DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07902">
        <property type="entry name" value="Adenylation_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18431">
        <property type="entry name" value="BRCT_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07967">
        <property type="entry name" value="OBF_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000002">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000085">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.10:FF:000001">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000003">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000032">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031916">
        <property type="entry name" value="LIG3_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16759">
        <property type="entry name" value="LIG3_BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00347">
        <property type="entry name" value="ZF_PARP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 21" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15 21" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="16 21" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 21" id="KW-0436">Ligase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PARP-type" evidence="24">
        <location>
            <begin position="95"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="25">
        <location>
            <begin position="587"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="26">
        <location>
            <begin position="935"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="844"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="864"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="913"/>
            <end position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00065554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1011" mass="113081" checksum="AE39AF26FD65A7B1" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BMH1</accession>
    <name>A0A673BMH1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ISL LIM homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">isl2b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BMH1"/>
    <dbReference type="Ensembl" id="ENSSORT00005043517.1">
        <property type="protein sequence ID" value="ENSSORP00005042439.1"/>
        <property type="gene ID" value="ENSSORG00005019666.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BMH1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048665">
        <property type="term" value="P:neuron fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09366">
        <property type="entry name" value="LIM1_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09374">
        <property type="entry name" value="LIM2_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000034">
        <property type="entry name" value="Insulin gene enhancer protein ISL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000041">
        <property type="entry name" value="insulin gene enhancer protein ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000068">
        <property type="entry name" value="Insulin gene enhancer protein ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047169">
        <property type="entry name" value="ISL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047244">
        <property type="entry name" value="ISL1/2-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204:SF2">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 11" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="25"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="88"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="190"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="192"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="331"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="364" mass="40751" checksum="2A0257E78656DC85" modified="2020-06-17" version="1">MVDIIFSSSFLGDMGDHSKKKPGFAMCVGCGSQIHDQYILRVSPDLEWHAACLKCAECSQYLDETCTCFVRDGKTYCKRDYVRLFGIKCAKCNLGFSSSDLVMRARDNVYHIECFRCSVCSRQLLPGDEFSLREDELLCRADHSLLLERSSAGSPISPGHIHSNRPLHLAADPVTVRQAPHRNHVHKQSEKTTRVRTVLNEKQLHTLRTCYNANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKKSILMKQLQQQQHSDKTVSIFNLQGLTGTPLVAGSPIRHENTVQGNPVEVQTYQPPWKALSEFALQSDLDQPAFQQLVSFSESGSLGNSSGSDVTSLSSQLPDTPNSMVPSPVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BNW2</accession>
    <name>A0A673BNW2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GNAS complex locus</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gnal</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-alpha family. G(s) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016645.1">
        <property type="nucleotide sequence ID" value="XM_030160785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BNW2"/>
    <dbReference type="Ensembl" id="ENSSORT00005044546.1">
        <property type="protein sequence ID" value="ENSSORP00005043450.1"/>
        <property type="gene ID" value="ENSSORG00005019990.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437550"/>
    <dbReference type="InParanoid" id="A0A673BNW2"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007191">
        <property type="term" value="P:adenylate cyclase-activating dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000720">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000003">
        <property type="entry name" value="Guanine nucleotide-binding protein G(S) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:006178">
        <property type="entry name" value="Guanine nucleotide-binding protein G(s) subunit alpha isoforms short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000367">
        <property type="entry name" value="Gprotein_alpha_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF233">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(OLF) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00443">
        <property type="entry name" value="GPROTEINAS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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        <location>
            <position position="54"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <location>
            <begin position="183"/>
            <end position="189"/>
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        <ligand>
            <name>GTP</name>
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        <ligand>
            <name>Mg(2+)</name>
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        <ligand>
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            <dbReference type="ARBA" id="ARBA00007172"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043450.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="44398" checksum="09B324C2F3FD69AB" modified="2020-06-17" version="1">MGCLGNSKTEDQRIDEKAQREANKKIEKQLQKERQAYKATHRLLLLGAGESGKSTIVKQMRILHVNGFNAEEKKQKIQDIRKNVKDAIVTIVSAMSTLIPPVPLANPEDQFRIEYIKSIAPLSDFDYSQEFFDHAKKLWDDEGVKACFERSNEYQLIDCAQYFLDRIDSVRQSDYTPTDQDLLRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNNTNRLREALDLFRSIWNNRWLRTISVILFLNKQDMLAEKVLAGKSKIEDYFPEYARYTIPNEATPEPGEDPRVTRAKFFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BP26</accession>
    <name>A0A673BP26_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BP26"/>
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        <property type="protein sequence ID" value="ENSSORP00005043515.1"/>
        <property type="gene ID" value="ENSSORG00005020101.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
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        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="2">
        <location>
            <begin position="36"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="129" mass="14182" checksum="66297ACCA9723F82" modified="2020-06-17" version="1">MNQLSVQIAQSSLTQVQVPAQPAEPVPSVSSARVPDEPNIPPPNKYSGNPETCRNFFTQIQLIIEAQPRRFSNDSAKIAYVASLLEGPPLSYFNALLEQESPVARSYSRLCAELKRVYDHPVRGWASGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQA7</accession>
    <name>A0A673BQA7_9TELE</name>
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        <submittedName>
            <fullName evidence="13">Ribonuclease ZC3H12A-like</fullName>
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        <name evidence="13" type="primary">zc3h12ab</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Kurtiformes</taxon>
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            <taxon>Apogoninae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <name>Mg(2+)</name>
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    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H12 family.</text>
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        <property type="interactions" value="1004"/>
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        <property type="protein sequence ID" value="ENSSORP00005042807.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18729">
        <property type="entry name" value="PIN_Zc3h12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rege-1_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040757">
        <property type="entry name" value="Regnase_1/ZC3H12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF10">
        <property type="entry name" value="ENDORIBONUCLEASE ZC3H12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18561">
        <property type="entry name" value="Regnase_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18039">
        <property type="entry name" value="UBA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="360"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
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            <end position="434"/>
        </location>
    </feature>
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        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="420"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010922"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="651" mass="72964" checksum="A6B1651A8FDAB4D0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BQM8</accession>
    <name>A0A673BQM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Lissencephaly-1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC115432951</name>
        <name evidence="15" type="synonym">LIS1</name>
        <name evidence="15" type="synonym">PAFAH1B1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. May be required for proliferation of neuronal precursors and neuronal migration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Can self-associate. Component of the cytosolic PAF-AH (I) heterotetrameric enzyme, which is composed of PAFAH1B1 (beta), PAFAH1B2 (alpha2) and PAFAH1B3 (alpha1) subunits. The catalytic activity of the enzyme resides in the alpha1 (PAFAH1B3) and alpha2 (PAFAH1B2) subunits, whereas the beta subunit (PAFAH1B1) has regulatory activity. Trimer formation is not essential for the catalytic activity. Interacts with dynein, dynactin, nde1 and ndel1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Can self-associate. Interacts with dynein, dynactin, NDE1 and NDEL1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
            <location evidence="15">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
            <location evidence="15">Cytoskeleton</location>
            <location evidence="15">Microtubule organizing center</location>
            <location evidence="15">Centrosome</location>
        </subcellularLocation>
        <text evidence="15">Localizes to the plus end of microtubules and to the centrosome.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Dimerization mediated by the LisH domain may be required to activate dynein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BQM8"/>
    <dbReference type="FunCoup" id="A0A673BQM8">
        <property type="interactions" value="1825"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005046197.1">
        <property type="protein sequence ID" value="ENSSORP00005045061.1"/>
        <property type="gene ID" value="ENSSORG00005020697.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BQM8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048854">
        <property type="term" value="P:brain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000038">
        <property type="entry name" value="Lissencephaly-1 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000002">
        <property type="entry name" value="Platelet-activating factor acetylhydrolase ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056795">
        <property type="entry name" value="PAC1-like_LisH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24951">
        <property type="entry name" value="LisH_PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037647">
        <property type="entry name" value="Dynein_regulator_Lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 15" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2 15" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 15" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 15" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10 15" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 15" id="KW-0813">Transport</keyword>
    <keyword evidence="4 16" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PAC1-like LisH-like dimerisation" evidence="17">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="237"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="383"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="417" mass="47115" checksum="9A5BFA97B0B72F73" modified="2020-06-17" version="1">FVLSQGKKKNSVQVYTTANRAIADYLRSNGYEEAYSTFKKEAELDMNEEVDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEMTHGGSVGQKRDPKEWIPRPPERYALSGHRAPVTRVIFHPVFSVMVTASEDATIKVWDYEAGDFERTLKGHTDSVQDISFDQTGKLLASCSADMSIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVATGYCVKTFTGHREWVRMVRPNQDGSLIASCSNDQTVRVWVVASKECKAELREHEHVVECIAWAPDSAHPTILEATGSESKKSGKPGPFLLSGSRDKTIKMWDVSIGICLMTLVGHDNWVRGVLVHPGGRFIVSCADDKTLRIWDYKNKRCMKTLSAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BRI3</accession>
    <name>A0A673BRI3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Interferon regulatory factor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">irf7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BRI3"/>
    <dbReference type="FunCoup" id="A0A673BRI3">
        <property type="interactions" value="874"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043908.1">
        <property type="protein sequence ID" value="ENSSORP00005042818.1"/>
        <property type="gene ID" value="ENSSORG00005019776.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BRI3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000631">
        <property type="entry name" value="Interferon regulatory factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000015">
        <property type="entry name" value="Interferon regulatory factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001346">
        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019471">
        <property type="entry name" value="Interferon_reg_factor-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF2">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10401">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01243">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="1">
        <location>
            <begin position="7"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="427" mass="49294" checksum="57BE9E22C91F3B49" modified="2020-06-17" version="1">QQCFPPKPQFANWLIEQVETGQYPGLSFVDHNKFRVPWKHNSRKDCNDGDSKIFRAWAVASGKINEFPNDKARWKTNFRCALNNLSTRFKMIKDNSKTNNEDPHKIYEIINTENLVIQDSQEDSDMAPDIITPTEYFPSGSEVCYCLEHTFTSLSQQWDKNYIQPNVPVMENYAPIPAPENHSQLLPVQPVYCEVNPPQFITQPLQPTIFDLEISIHYRKKEMLKTTLHTPRLQLHYYHEAPELNAEHLCFPSTESLLDHKQIEYTNRILNSIQKGLLLEVRETGIYAFRQDRCHVFASTSDPVVAHPDPRKLPQNTTVELLSFEKYVNELKQFRENNGGSPEYTINMCFGEKFPDGKPLEKKLIVVKVVPLICRHFHELAQMEGASSLHSANVSLQISHNSLYDLISSVFGLPMVEDPAHPFFLHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSD0</accession>
    <name>A0A673BSD0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hint1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine 5'-phosphoramidate + H2O = NH4(+) + AMP</text>
            <dbReference type="Rhea" id="RHEA:67916"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57890"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HINT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030011996.1">
        <property type="nucleotide sequence ID" value="XM_030156136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BSD0"/>
    <dbReference type="FunCoup" id="A0A673BSD0">
        <property type="interactions" value="1325"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005045209.1">
        <property type="protein sequence ID" value="ENSSORP00005044089.1"/>
        <property type="gene ID" value="ENSSORG00005020322.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434275"/>
    <dbReference type="CTD" id="3094"/>
    <dbReference type="InParanoid" id="A0A673BSD0"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01276">
        <property type="entry name" value="PKCI_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000005">
        <property type="entry name" value="Histidine triad nucleotide-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="6">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="4 5">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="3">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005044089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="126" mass="13515" checksum="A8B63362AC27A1AD" modified="2020-06-17" version="1">MADETAKAQTAQPGGDTIFGKIIRREIPATFIHEDDLCVAFNDVAPQAPTHILVVPKKPIVQLSKAEEGDAALLGHLMIVAKKCAEQAGLTRGYRVVVNDGADGGQSVYHIHLHVLGGRQMQWPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BSD1</accession>
    <name>A0A673BSD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Synapse-associated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">BSD domain-containing signal transducer and Akt interactor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115417831</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a role in adipocyte differentiation by promoting mTORC2-mediated phosphorylation of AKT1 at 'Ser-473' after growth factor stimulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts (via phosphorylated form and BSD domain) with AKT1; this interaction is enhanced in a mTORC2-mediated manner in response to epidermal growth factor (EGF) stimulation and activates AKT1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BSD1"/>
    <dbReference type="Ensembl" id="ENSSORT00005046861.1">
        <property type="protein sequence ID" value="ENSSORP00005045706.1"/>
        <property type="gene ID" value="ENSSORG00005020986.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BSD1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071364">
        <property type="term" value="P:cellular response to epidermal growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045600">
        <property type="term" value="P:positive regulation of fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3970.10:FF:000001">
        <property type="entry name" value="synapse-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051494">
        <property type="entry name" value="BSD_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019:SF6">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="7">
        <location>
            <begin position="166"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="236"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00065448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="359" mass="40844" checksum="EF348A3891B3A12D" modified="2020-06-17" version="1">MFKNWGTWLGIEHDNDQVKQESESDVDNNGDKKPEVSPATGPAEAQQGSHGDAQRPQLVQKTKGFSEYIYSFASSASKKFSESVAETAQTLKKSVEEGKINGIMDKTILGDFQKEQEKFVQEKKAKKSGAALPPWVGYNEEETMQQQILALSSDQRNFLRDPPAGVHFHFDFEQMYPVAMVMLEEDELLRKMRFHLVPKQVKEEAFWKNYFYRVSLIKQSAQLTALAAQQAAEWKDKEKAGSSPEAVHQNDGVKRKGAPAISSSKPKSSEDEEEISTSPPVAEFVSDAFDSCKINEDDLRKEMEQLVLDKREHANPQSETADWERELQEELQEYDVLAEDENHDEGWDKEIEDMLKEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BT06</accession>
    <name>A0A673BT06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gamma-secretase-activating protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BT06"/>
    <dbReference type="Ensembl" id="ENSSORT00005047111.1">
        <property type="protein sequence ID" value="ENSSORP00005045956.1"/>
        <property type="gene ID" value="ENSSORG00005021074.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BT06"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902004">
        <property type="term" value="P:positive regulation of amyloid-beta formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028010">
        <property type="entry name" value="GSAP_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026172">
        <property type="entry name" value="GSAP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13630">
        <property type="entry name" value="GAMMA-SECRETASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13630:SF1">
        <property type="entry name" value="GAMMA-SECRETASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14959">
        <property type="entry name" value="GSAP-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-secretase-activating protein C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="128" mass="14808" checksum="1F70F72EB1F461EB" modified="2020-06-17" version="1">MFRILEAARSLCLPLPPGYNTLLAVFAVRCLPHPTFLQYIDHKLLQLTETFVSRPMEQTIYHMWDHPVSSASISRDYVRTLLEKHNKNKSFSCVPAANPFEEQENVDARFVEETALKQTLIQLGFEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BTL0</accession>
    <name>A0A673BTL0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rap-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ralgapa2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029981962.1">
        <property type="nucleotide sequence ID" value="XM_030126102.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A673BTL0">
        <property type="interactions" value="1045"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047345.1">
        <property type="protein sequence ID" value="ENSSORP00005046186.1"/>
        <property type="gene ID" value="ENSSORG00005017617.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413331"/>
    <dbReference type="CTD" id="57186"/>
    <dbReference type="InParanoid" id="A0A673BTL0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000001">
        <property type="entry name" value="Ral GTPase-activating protein subunit alpha-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046859">
        <property type="entry name" value="RGPA/RALGAPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF2">
        <property type="entry name" value="RAL GTPASE-ACTIVATING PROTEIN SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20412">
        <property type="entry name" value="RALGAPB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="4">
        <location>
            <begin position="1722"/>
            <end position="1930"/>
        </location>
    </feature>
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        <location>
            <begin position="339"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="715"/>
            <end position="866"/>
        </location>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1973"/>
            <end position="2011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="756"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="778"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="857"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1567"/>
            <end position="1576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1973"/>
            <end position="1984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1991"/>
            <end position="2001"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="2011" mass="225864" checksum="69D04D37C1C3B648" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BUH5</accession>
    <name>A0A673BUH5_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Tubulin--tyrosine ligase-like protein 12 SET-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ttll12</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BUH5"/>
    <dbReference type="Ensembl" id="ENSSORT00005044942.1">
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        <property type="gene ID" value="ENSSORG00005020224.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BUH5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057954">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR027749">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25556">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="2">
        <location>
            <begin position="69"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="638" mass="73977" checksum="00C8C2BF340CFFA0" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BVL9</accession>
    <name>A0A673BVL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-microseminoprotein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-microseminoprotein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BVL9"/>
    <dbReference type="Ensembl" id="ENSSORT00005046310.1">
        <property type="protein sequence ID" value="ENSSORP00005045174.1"/>
        <property type="gene ID" value="ENSSORG00005020747.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BVL9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1900">
        <property type="entry name" value="Beta-microseminoprotein (PSP94) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008735">
        <property type="entry name" value="PSP94"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10500">
        <property type="entry name" value="BETA-MICROSEMINOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10500:SF7">
        <property type="entry name" value="BETA-MICROSEMINOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05825">
        <property type="entry name" value="PSP94"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025350727" description="Beta-microseminoprotein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005045174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11615" checksum="1F711D27B055995B" modified="2020-06-17" version="1">QKYFSLALLLCALAALAHAFCYQKEVKPGMTHCVDDADGTIHEVGSSWRNSKCMDCTCSGCCTGYATPRDFSDDCVSVFDPVACKYIVHKKDDKTKLCDIYSAVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BXW6</accession>
    <name>A0A673BXW6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Si:ch211-102l7.3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115417206</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BXW6"/>
    <dbReference type="Ensembl" id="ENSSORT00005047771.1">
        <property type="protein sequence ID" value="ENSSORP00005046610.1"/>
        <property type="gene ID" value="ENSSORG00005021339.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BXW6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051659">
        <property type="entry name" value="Serine_Protease_S1-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24254:SF9">
        <property type="entry name" value="INACTIVE SERINE PROTEASE PAMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24254">
        <property type="entry name" value="PROTHROMBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="CUB" evidence="9">
        <location>
            <begin position="87"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="225"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="335"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="374"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="646" mass="71960" checksum="3C4689DDEB7F7306" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1V8</accession>
    <name>A0A673C1V8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nvl</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030011659.1">
        <property type="nucleotide sequence ID" value="XM_030155799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C1V8"/>
    <dbReference type="Ensembl" id="ENSSORT00005048418.1">
        <property type="protein sequence ID" value="ENSSORP00005047244.1"/>
        <property type="gene ID" value="ENSSORG00005020896.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115434103"/>
    <dbReference type="CTD" id="4931"/>
    <dbReference type="InParanoid" id="A0A673C1V8"/>
    <dbReference type="OrthoDB" id="2187at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000052">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000600">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038100">
        <property type="entry name" value="NLV2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031996">
        <property type="entry name" value="NVL2_nucleolin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16725">
        <property type="entry name" value="Nucleolin_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="263"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="606"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="83"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="471"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="858" mass="94368" checksum="0068C3B7FD5D8064" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5F2</accession>
    <name>A0A673C5F2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ywhaq</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029982469.1">
        <property type="nucleotide sequence ID" value="XM_030126609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C5F2"/>
    <dbReference type="Ensembl" id="ENSSORT00005050404.1">
        <property type="protein sequence ID" value="ENSSORP00005049205.1"/>
        <property type="gene ID" value="ENSSORG00005022358.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413618"/>
    <dbReference type="InParanoid" id="A0A673C5F2"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000001">
        <property type="entry name" value="14-3-3 gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="3"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="248" mass="27980" checksum="56C09B624D6961A7" modified="2020-06-17" version="1">MDRSDYIQQAKLAEQAERYDDMADFMKKVTELGGDLSNEERNLLSVAYKNVVGARRSSWRVISALHTKVDGTDKKQQMIKEYRETVEKELEDICHDVLRLLDEHLISTASPAESQVFYLKMKGDYYRYLAEVASGEGKEKATDNAQKSYTAAYEVSQKDMEPTHPIRLGLALNFSVFYYEILNSPERACNLAKTAFDEAIAELDKLGEDSYKDSTLIMQLLRDNLTLWTSDNAAEEGDGGEAGEGGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5I1</accession>
    <name>A0A673C5I1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protocadherin beta-16-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115426758</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C5I1"/>
    <dbReference type="Ensembl" id="ENSSORT00005048702.1">
        <property type="protein sequence ID" value="ENSSORP00005047523.1"/>
        <property type="gene ID" value="ENSSORG00005021730.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C5I1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF296">
        <property type="entry name" value="PROTOCADHERIN 1 GAMMA 11 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="696"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="37"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="144"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="253"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="365"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="462"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="586"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="827" mass="91868" checksum="A75CE6441C0EEC44" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5K4</accession>
    <name>A0A673C5K4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115437151</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673C5K4"/>
    <dbReference type="Ensembl" id="ENSSORT00005048733.1">
        <property type="protein sequence ID" value="ENSSORP00005047553.1"/>
        <property type="gene ID" value="ENSSORG00005021722.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C5K4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
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        <property type="entry name" value="Putative ras-related protein rab-11a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="229" mass="25775" checksum="3F1FBA238DC8510E" modified="2020-06-17" version="1">MQKTCSSLQIRFSRYKVQTGRKNLFVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADNNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIAERSAHDESPGNNVVDISVPPTTDGQRGSKLQCCQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C707</accession>
    <name>A0A673C707_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC115411842</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C707"/>
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        <property type="interactions" value="141"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005049024.1"/>
        <property type="gene ID" value="ENSSORG00005022286.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C707"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Claudin"/>
    </dbReference>
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        <property type="entry name" value="Claudin10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01383">
        <property type="entry name" value="CLAUDIN10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025358690" description="Claudin" evidence="9">
        <location>
            <begin position="22"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="98"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="247" mass="27067" checksum="F7E0070F3EF7DEE8" modified="2020-06-17" version="1">MFQEILAFILAASGWVLVTSTLPTDYWKVSTLDGSVTITNTYWSNLWKSCVTDSTGSSSCKDFPSMLALDGWLFFLACRGLMIAAVCLGFFGSVFALVGMKCTKIGGSGKSKSRVTCFAGIDFILSGCSLSGCSLYAYKITSEFYDPMVVEMKYELGAALFIGWAGSILCILGGIALCFSIVQSPKGLYEQKRYTVTVLYVADLVGHYSDFYVKMNLNLHFLSLQSYSSFSTLNTGLQVKCFQNKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C7Q5</accession>
    <name>A0A673C7Q5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Solute carrier family 45 member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">slc45a2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030004130.1">
        <property type="nucleotide sequence ID" value="XM_030148270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C7Q5"/>
    <dbReference type="FunCoup" id="A0A673C7Q5">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051239.1">
        <property type="protein sequence ID" value="ENSSORP00005050035.1"/>
        <property type="gene ID" value="ENSSORG00005022705.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429034"/>
    <dbReference type="CTD" id="51151"/>
    <dbReference type="InParanoid" id="A0A673C7Q5"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17313">
        <property type="entry name" value="MFS_SLC45_SUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF34">
        <property type="entry name" value="MEMBRANE-ASSOCIATED TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="62220" checksum="D4151AAD8185BBFF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAP0</accession>
    <name>A0A673CAP0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cyclosome subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">anapc5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the APC5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CAP0"/>
    <dbReference type="Ensembl" id="ENSSORT00005053884.1">
        <property type="protein sequence ID" value="ENSSORP00005052636.1"/>
        <property type="gene ID" value="ENSSORG00005023699.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CAP0"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16270">
        <property type="entry name" value="Apc5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048968">
        <property type="entry name" value="Apc5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21371">
        <property type="entry name" value="Apc5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 5 N-terminal" evidence="20">
        <location>
            <begin position="30"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="19">
        <location>
            <begin position="268"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="19">
        <location>
            <begin position="615"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="184"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="782" mass="88572" checksum="696C6194B5A40E1E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAX1</accession>
    <name>A0A673CAX1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CAX1"/>
    <dbReference type="Ensembl" id="ENSSORT00005052439.1">
        <property type="protein sequence ID" value="ENSSORP00005051215.1"/>
        <property type="gene ID" value="ENSSORG00005023144.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CAX1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF17">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="23"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="94" mass="10871" checksum="A7EF441B07F8A8A8" modified="2020-06-17" version="1">MSLTSWFLVSGGGTRHRLPREMIFVGRDDCELMLQSRSVDKQHAVINYEPNTDEHKVKDLGSLNGTFVNDVRIQEQIYVTLKIDDKLRFGYDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBN0</accession>
    <name>A0A673CBN0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase 8 domain containing 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glt8d2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030006472.1">
        <property type="nucleotide sequence ID" value="XM_030150612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CBN0"/>
    <dbReference type="Ensembl" id="ENSSORT00005051924.1">
        <property type="protein sequence ID" value="ENSSORP00005050709.1"/>
        <property type="gene ID" value="ENSSORG00005022976.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430539"/>
    <dbReference type="CTD" id="83468"/>
    <dbReference type="InParanoid" id="A0A673CBN0"/>
    <dbReference type="OrthoDB" id="411524at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050748">
        <property type="entry name" value="Glycosyltrans_8_dom-fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025371736" description="Glycosyltransferase 8 domain containing 2" evidence="4">
        <location>
            <begin position="27"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41279" checksum="2ABBDF3A75854EE9" modified="2020-06-17" version="1">MPLLRKINRVLLVLLVLMVCLLLHSALRVSTQPKASDQWRGLSHGAGLPGAKTPQEENVIPVVICASEERLGATMATINSVYSNTDASVLFYLVTLRDAVKLTRQYIERTKLKGIRYKILEFNPMILRGKVKPDSSRPDLLHPLNFVRFYLPMLEINHKRVIYLDDDVIVQGDIKDLFNIKMKPGHAAAFATDCDLPSTHEVVRSIGMQTTYMGFLDYRKQEVKDLGINPSDCSFNPGVFVADIREWKKQKITKQLEKWMEENFRINIYSSAMAGGVATPPMLIVFHDKFTTLDPMWHVRHLGWSPDAHYSDNILQKARLLHWNGPFKPWSYPAVHLDLWEKWFVPDPSAKFTLLRPDGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBN7</accession>
    <name>A0A673CBN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Creatine kinase S-type, mitochondrial</fullName>
            <ecNumber evidence="3">2.7.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Sarcomeric mitochondrial creatine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115425637</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15 17">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_029999159.1">
        <property type="nucleotide sequence ID" value="XM_030143299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CBN7"/>
    <dbReference type="FunCoup" id="A0A673CBN7">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005050750.1">
        <property type="protein sequence ID" value="ENSSORP00005049548.1"/>
        <property type="gene ID" value="ENSSORG00005022484.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425637"/>
    <dbReference type="InParanoid" id="A0A673CBN7"/>
    <dbReference type="OrthoDB" id="430219at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000002">
        <property type="entry name" value="creatine kinase S-type, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000002">
        <property type="entry name" value="Creatine kinase S-type, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF19">
        <property type="entry name" value="CREATINE KINASE S-TYPE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="18">
        <location>
            <begin position="42"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="19">
        <location>
            <begin position="156"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="159"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="323"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="351"/>
            <end position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="415" mass="46882" checksum="34E466883988DF46" modified="2020-06-17" version="1">MANSFTRMMSGRNTAVMLASVGAGALASGYLLSDMTAAAERRRAYPPSADFPDLRKHNNCMASALTPTIYARLRDKTTPNNWTIDQCIQTGVDNPGHPFIKTVGMVAGDEESYEVFAELFNPVIKDRHNGYDPRTMKHPTDLDASKISSGMFDERYVLSSRVRTGRSIRGLSLPPACTRSERREVERVVVTALSGLKGDLSGRYYSLIDMTDKEQQQLIDEHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKNFLIWINEEDHTRIISMEKGGNMKRVFERFCRGLKQVEQLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIRLPFLSRDPRFKKILENLRLQKRGTGGVDTAASGDTVDISNLDRLGKSEVELVQLVIDGVNYLIECEKRLERGQDIKIPSPIPQFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFH1</accession>
    <name>A0A673CFH1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">La ribonucleoprotein 4Ab</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CFH1"/>
    <dbReference type="Ensembl" id="ENSSORT00005053304.1">
        <property type="protein sequence ID" value="ENSSORP00005052064.1"/>
        <property type="gene ID" value="ENSSORG00005023489.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CFH1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08035">
        <property type="entry name" value="LARP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058699">
        <property type="entry name" value="RRM_LARP4/4B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF48">
        <property type="entry name" value="LA-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26088">
        <property type="entry name" value="RRM_LARP4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="5">
        <location>
            <begin position="65"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="321"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="378" mass="43058" checksum="F2CCECAC054EBFB4" modified="2020-06-17" version="1">MTDGYADVSSSVDKAYSTEYPEGSNYDHVPTDCIKNDIDYPDLGYLVFDPQYNTAIEDDVSKEQPMSEESLRESLKKQLEFCFSRENLSKDLYLISQMDSDQFVPIWTIACMEDIKSLTTDMDLILDVLKASPMVQVDESGEKVRPNHSRCIIILREVPETTPVEEVEALFKSENWMHLRAYRYLREEVKMFQGKPIMARIKAINTFFGKSGFRSVESGVYGQHTQPQAQFGTPVYMQQMYSPQQQYPVYPVVSPTWSPSPMPYFETPLAPFPNGGFVNGYSSPGNYKANPSSVNAQRPFHFIFTTAVVTLVMDQALRYINAHAPPPHPPPPPAPTKKKKNPRAHRHTQYTQCPKQFVLCPKINNLVHCVVKDSLQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFQ8</accession>
    <name>A0A673CFQ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CFQ8"/>
    <dbReference type="Ensembl" id="ENSSORT00005052139.1">
        <property type="protein sequence ID" value="ENSSORP00005050923.1"/>
        <property type="gene ID" value="ENSSORG00005023042.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CFQ8"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000210">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000061">
        <property type="entry name" value="Glutamate receptor, ionotropic kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 17" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 17" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="18">
        <location>
            <begin position="22"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="19">
        <location>
            <begin position="37"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="15">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="15">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="321"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="473" mass="53150" checksum="51C2D00B913DD612" modified="2020-06-17" version="1">KLLDHQKSTKTMVMQSSTNSCVYHVTKTEKKTWNENPYVMYKKSDKPLYGNDRFEGYCLDLLKELSNILGFSYEVKLVSDGKYGAQNDKGEWNGMVADLAVAPLTITYVREKVIDFSKPFMTLGISILYHKPNGTNPGVFSFLNPLSPDIWMYVLLACLGVSCVFTPYEWYNPHPCNPDSDVVENNFTLINSVWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTDGLTIDSADDLQNFHGMTRPTLPLIGSSVLMPTKSKISTYEKMWAFMSSRKNTALVKNNREGIQRVLTTDYALLMESTSIEYISQRNCNLTQIGGLIDSKGYGVGTPIGSPYRDKVTIAILQLQEEGKLHMMKEKWWRGNGCPEEDSKEASALGVENIGGIFIVLAAGLVLSVFVAIGEFIYKSRKNLDIEEVSVASSSSSSEVYTYHWTNNSLKSECVELHCYNQNKRLFNRGVNLVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CG29</accession>
    <name>A0A673CG29_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBC1 domain family member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tbc1d1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005052267.1">
        <property type="protein sequence ID" value="ENSSORP00005051048.1"/>
        <property type="gene ID" value="ENSSORG00005023019.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CG29"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00934">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01269">
        <property type="entry name" value="PTB_TBC1D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000003">
        <property type="entry name" value="Putative TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000001">
        <property type="entry name" value="TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2750:FF:000001">
        <property type="entry name" value="TBC1 domain family member 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021785">
        <property type="entry name" value="DUF3350"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF18">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3350"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="389"/>
            <end position="468"/>
        </location>
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        <location>
            <begin position="915"/>
            <end position="1109"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
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        </location>
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        <location>
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            <end position="693"/>
        </location>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051048.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1294" mass="143549" checksum="EC1B86E84691C43C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054841.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="198" mass="21972" checksum="50E33001D8044158" modified="2020-06-17" version="1">MDDTLFQLKFTSKQLERLAKKAEKESEKEQAKVKKALQQKNVDCARVYAENAIRKKNEGINWLRMASRVDAVASKVQTAVTMKGVTKNMGQVTKALDKALGSMDLQKVSAVMDKFETQVQNLDVHTSVMEDSMSSATTLTTPQDQVDDLIHQIAEESGLEVMDQLNQLPEGATSVGAESARSQDKEDQLSRRLAALRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CKU5</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized LOC115413409</fullName>
        </submittedName>
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        <name evidence="2" type="primary">si:dkey-16j16.4</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="match status" value="1"/>
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    </dbReference>
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            <end position="44"/>
        </location>
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            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="31428" checksum="9AAAD560D3BF5448" modified="2020-06-17" version="1">MLKTLTKKLRRHSLNEIHPFQLKISYHGSGEGGESDDSEGENQELAQIDRERRRNCALTPLATSQQHNQGPLSPARLRRLRLLLDANLDRHSSEEELERISCGDNRKWVSAFPQRHSDHHSSASSDEEVRDLCGCGSPAASNRPLVESGACLAASPSPVLFSSSPPRSLKPPSMRFQLQVVQPVTRPIILTHFDQSAPYRKYRHSYGGEPGRPSLDLEKMQQKMLLKKNCGGKTRTIKIRNLTGSRPPPRYTYDPSIFAFRSLSTVPPCSPLNPTEEPTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CQF5</accession>
    <name>A0A673CQF5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Potassium channel tetramerisation domain containing 12.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CQF5"/>
    <dbReference type="FunCoup" id="A0A673CQF5">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057807.1">
        <property type="protein sequence ID" value="ENSSORP00005056510.1"/>
        <property type="gene ID" value="ENSSORG00005025133.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CQF5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein homooligomerization"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="H1_KCTD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000031">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049905">
        <property type="entry name" value="H1_KCTD12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057093">
        <property type="entry name" value="H1_KCTD8_12_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF29">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23110">
        <property type="entry name" value="H1_KCTD8_12_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="15"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="281" mass="32042" checksum="0ED12CDC6BDB0374" modified="2020-06-17" version="1">PQLSNCGDSCAPFSEIIELNVGGQVYVTRHKTLIAVPDSLLWNMFSKKSPKELARDSKGRFFLDRDGFLFRYILDYLRDLNLVLPDYFPEKSRLQREADFFQLQDLAKRLSPRMSKDNSISEEISQSDTEEGAQQCGSSGGMETLRTMSVSGAMRSPSLDSRKSGYITIGYRGSYTIGRDIQTDAKFRRVARITVCGKTSLAKEVFGDTLNESRDPDRPPERYTSRYYLKYNFLEQAFDKLTEVGFHMVACSSTGTCAYTSNDPNEDKIWTSYTEYVFCRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRE1</accession>
    <name>A0A673CRE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115438693</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030018334.1">
        <property type="nucleotide sequence ID" value="XM_030162474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CRE1"/>
    <dbReference type="Ensembl" id="ENSSORT00005059519.1">
        <property type="protein sequence ID" value="ENSSORP00005058196.1"/>
        <property type="gene ID" value="ENSSORG00005025707.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115438693"/>
    <dbReference type="InParanoid" id="A0A673CRE1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13284">
        <property type="entry name" value="PH_OSBP_ORP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000074">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000003">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF205">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="13"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="654"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="263"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="662"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="760" mass="86035" checksum="CB0F224228DF5682" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CRZ8</accession>
    <name>A0A673CRZ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Septin-9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC115421406</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029993151.1">
        <property type="nucleotide sequence ID" value="XM_030137291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CRZ8"/>
    <dbReference type="FunCoup" id="A0A673CRZ8">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005059749.1">
        <property type="protein sequence ID" value="ENSSORP00005058426.1"/>
        <property type="gene ID" value="ENSSORG00005025787.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115421406"/>
    <dbReference type="CTD" id="541494"/>
    <dbReference type="InParanoid" id="A0A673CRZ8"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000143">
        <property type="entry name" value="septin-9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="10">
        <location>
            <begin position="299"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="68"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="156"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005058426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="617" mass="69444" checksum="E14A5985C5FD1D77" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CT49</accession>
    <name>A0A673CT49_9TELE</name>
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        <recommendedName>
            <fullName evidence="19">Myosin X, like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">myo10l3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005057018.1">
        <property type="protein sequence ID" value="ENSSORP00005055732.1"/>
        <property type="gene ID" value="ENSSORG00005024490.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CT49"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044295">
        <property type="term" value="C:axonal growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13202">
        <property type="entry name" value="FERM_C_MyoX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17206">
        <property type="entry name" value="FERM_F1_Myosin-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14873">
        <property type="entry name" value="MYSc_Myo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13296">
        <property type="entry name" value="PH2_MyoX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13297">
        <property type="entry name" value="PH3_MyoX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.530:FF:000001">
        <property type="entry name" value="Pleckstrin homology domain-containing family H member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000008">
        <property type="entry name" value="Putative unconventional myosin-IXa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051724">
        <property type="entry name" value="Actin_motor_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031971">
        <property type="entry name" value="MYO10_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041797">
        <property type="entry name" value="MyoX_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040640">
        <property type="entry name" value="MyoX_N_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036124">
        <property type="entry name" value="MYSc_Myo10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46049">
        <property type="entry name" value="AGAP003327-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46049:SF4">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16735">
        <property type="entry name" value="MYO10_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18597">
        <property type="entry name" value="SH3_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="64"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="1211"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="1382"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="15">
        <location>
            <begin position="1525"/>
            <end position="1685"/>
        </location>
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    <feature type="domain" description="FERM" evidence="13">
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            <end position="2036"/>
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    </feature>
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        <location>
            <begin position="1694"/>
            <end position="1737"/>
        </location>
    </feature>
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        <location>
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            <end position="644"/>
        </location>
    </feature>
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        <location>
            <begin position="954"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="800"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1095"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="158"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2051" mass="236723" checksum="6B0F34299A48B18B" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CX92</accession>
    <name>A0A673CX92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Short transient receptor potential channel 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">trpc1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <end position="646"/>
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        <location>
            <begin position="192"/>
            <end position="254"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009944"/>
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    </evidence>
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</entry>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="NEURONAL PENTRAXIN RECEPTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR19277">
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    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
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            <end position="551"/>
        </location>
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            <end position="56"/>
        </location>
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            <end position="289"/>
        </location>
    </feature>
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        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
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        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="278"/>
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        </location>
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        <location>
            <begin position="381"/>
            <end position="440"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="559" mass="60170" checksum="2251235CAD75FF27" modified="2025-10-08" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3L7</accession>
    <name>A0A672Y3L7_9TELE</name>
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        <recommendedName>
            <fullName evidence="12">Palmitoyltransferase</fullName>
            <ecNumber evidence="12">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">zdhhc13</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 12">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.</text>
    </comment>
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        <property type="gene ID" value="ENSSORG00005000268.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF16">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC13"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
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        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="212"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="444"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="636" mass="71149" checksum="5DF6CCBB409982EC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y3M7</accession>
    <name>A0A672Y3M7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methylmalonic aciduria and homocystinuria type D homolog, mitochondrial-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mmadhca</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Y3M7"/>
    <dbReference type="FunCoup" id="A0A672Y3M7">
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005000834.1">
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        <property type="gene ID" value="ENSSORG00005000501.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y3M7"/>
    <dbReference type="OrthoDB" id="10263782at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
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    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019362">
        <property type="entry name" value="MMADHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13192:SF2">
        <property type="entry name" value="METHYLMALONIC ACIDURIA (COBALAMIN DEFICIENCY) CBLD TYPE, WITH HOMOCYSTINURIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13192">
        <property type="entry name" value="MY011 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10229">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="294" mass="32325" checksum="065D8E73AEEBC2FC" modified="2020-06-17" version="1">MSSTVLCGRGRLVLSQTTARKALAALRVTRTRNFSAASSDEPYIAVSPADSGPRTVWPDENMGPFGPQDQRFQLPGNVGFDCHLEGVTDQKKPPVHKTVPDVLTAPSSTERHQFILAQFVNEFHGKLGPISTRVHKAEDYFNLTETDCSISSCPELLKKDLELLFPSAPPTAITVVTVTQKEVQQDKDQLRNRFVSGAKEMCFALWTAGYWADFIDPTTGAAFFASPSSQTTLRTEEELRHLGFHIEVSASCTVIRHILRGTPMFVGTVFTNAPTQSTTIARLQGLGNEPDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y4A0</accession>
    <name>A0A672Y4A0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear protein 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NUPR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y4A0"/>
    <dbReference type="Ensembl" id="ENSSORT00005000794.1">
        <property type="protein sequence ID" value="ENSSORP00005000768.1"/>
        <property type="gene ID" value="ENSSORG00005000479.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y4A0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018792">
        <property type="entry name" value="NUPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149:SF6">
        <property type="entry name" value="NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149">
        <property type="entry name" value="NUCLEAR PROTEIN 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10195">
        <property type="entry name" value="Phospho_p8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="102" mass="12254" checksum="A9E77C5A055FF435" modified="2020-06-17" version="1">WNHHESCRREEPEATSFEEEYYDQYEYYNLTDKYTEGSSRKGRTKKESSENTKRPKPGGHERKIVEKLQNSEKNRKQDQKQDRSENTEVSQNQNQRVELDPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y5A0</accession>
    <name>A0A672Y5A0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar protein 56</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nucleolar protein 5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nop56</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the early to middle stages of 60S ribosomal subunit biogenesis. Required for the biogenesis of box C/D snoRNAs such U3, U8 and U14 snoRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of a large pre-ribosomal ribonucleoprotein (RNP) complex, that consists of at least 62 ribosomal proteins, 45 nonribosomal proteins and both pre-rRNA and mature rRNA species. Within this complex directly interacts with TCOF1 in an RNA-independent manner. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles; the core proteins SNU13, NOP56, NOP58 and FBL or FBLL1 assemble stepwise onto the snoRNA. Interacts with NOP1 and NOP58. Interacts with NUFIP1, RUVBL1 and RUVBL2; RUVBL1:RUVBL2 seem to bridge the association of NOP56 with NUFIP1. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with NOP2 and FBL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029990688.1">
        <property type="nucleotide sequence ID" value="XM_030134828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y5A0"/>
    <dbReference type="FunCoup" id="A0A672Y5A0">
        <property type="interactions" value="1691"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001328.1">
        <property type="protein sequence ID" value="ENSSORP00005001293.1"/>
        <property type="gene ID" value="ENSSORG00005000761.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419804"/>
    <dbReference type="CTD" id="10528"/>
    <dbReference type="InParanoid" id="A0A672Y5A0"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000001">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000002">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="293"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="441"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="462"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="575" mass="63537" checksum="8BCDD85EF3D0D81D" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y7Q4</accession>
    <name>A0A672Y7Q4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Interferon lambda receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115412569</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029981002.1">
        <property type="nucleotide sequence ID" value="XM_030125142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y7Q4"/>
    <dbReference type="Ensembl" id="ENSSORT00005002607.1">
        <property type="protein sequence ID" value="ENSSORP00005002543.1"/>
        <property type="gene ID" value="ENSSORG00005001524.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115412569"/>
    <dbReference type="InParanoid" id="A0A672Y7Q4"/>
    <dbReference type="OrthoDB" id="10031784at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF84">
        <property type="entry name" value="INTERFERON ALPHA_BETA RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025370115" evidence="3">
        <location>
            <begin position="20"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="21"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
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            <begin position="396"/>
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    </feature>
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            <begin position="412"/>
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    </feature>
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            <end position="465"/>
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            <begin position="466"/>
            <end position="478"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="486" mass="53737" checksum="DD914A6F51A73D3A" modified="2020-06-17" version="1">MTALILMLIWLSQVFPVMSDLPQPLSVRLNSSHFHHMLQWEAGVGTPPGVYYKVTFLTQDKHSWNPVVGCEHVQHPLVCNMTVSLSDPQKYYFIRVVAVLDKHSSKAVESTAFHPIRDTILDPPLFTVTSCGLDLCLNFDPPMEHVRAIYNRFKYQLRIKSNNADEPRIREITSLNKEVLKDLPPGREYCISVRVSGFLGGDMQRTFNFSQPNCVIIPGHLNTDPPIAATLCLFVIFILVVLGLLYFAGFIWLKNTPLPLVLTSIHHTDEFLHTSPLVSLGSILTVKPTAPSSGKKKSSQTESSDSDEESVTDITGGRGGGCYKLRVGTNLLSTSSSSSSSSLSAPLSPNCFPTNQNTLSVPPEVFSALPKAPIAANTSTSTSTADSLTAERTEREEEEEKEEEGMREWGSQDVNLLTLTFGNHKEEKEEEPAPVDLQSAPPASEETSVLLSHMCHFSEDAKDSDICSDDEEEEDEYDGANGYMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y823</accession>
    <name>A0A672Y823_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC115416990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSSORP00005002847.1"/>
        <property type="gene ID" value="ENSSORG00005001772.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="DUF4643"/>
    </dbReference>
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        <property type="entry name" value="PROLINE-RICH PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38004:SF1">
        <property type="entry name" value="PROLINE-RICH PROTEIN 33"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="780"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="1322"/>
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        </location>
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        <location>
            <begin position="174"/>
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        </location>
    </feature>
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            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1344" mass="144937" checksum="38A4252F4552F833" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8A6</accession>
    <name>A0A672Y8A6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">dipeptidase E</fullName>
            <ecNumber evidence="10">3.4.13.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Asp-specific dipeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">si:dkey-69o16.5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolyzes dipeptides containing N-terminal aspartate residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Dipeptidase E catalyzes the hydrolysis of dipeptides Asp-|-Xaa. It does not act on peptides with N-terminal Glu, Asn or Gln, nor does it cleave isoaspartyl peptides.</text>
            <dbReference type="EC" id="3.4.13.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S51 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.21" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y8A6"/>
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    </dbReference>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="GAT1_Peptidase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase E"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005320">
        <property type="entry name" value="Peptidase_S51"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003642">
        <property type="entry name" value="PRK05282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20842:SF0">
        <property type="entry name" value="ALPHA-ASPARTYL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20842">
        <property type="entry name" value="PROTEASE S51 ALPHA-ASPARTYL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03575">
        <property type="entry name" value="Peptidase_S51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006534"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="239" mass="26582" checksum="2856B655F1E5DBC6" modified="2020-06-17" version="1">MKRRLLLVSNSTLHGSGYLDHCQQQIAQFFGKDVKRVLFVPYALHDRDAYTKTARNKFKTLGYEVDGIHEAADPVEAVRKAEGIFIGGGNTFRLLKSLYDNKVVSEIRKRVLEDGVPYMGSSAGTNVATISISTTNDMPIVYPPTFGAIGLVPFNINPHYLDPDPNSRHMGETREQRITQYHEETDTNVLGLREGCMLLVEGNKATLLGSTKARLFTRGKTCVEYDPGSDLSFLLTDAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8E0</accession>
    <name>A0A672Y8E0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">cAMP responsive element binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">creb1b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Y8E0"/>
    <dbReference type="Ensembl" id="ENSSORT00005003064.1">
        <property type="protein sequence ID" value="ENSSORP00005002973.1"/>
        <property type="gene ID" value="ENSSORG00005001847.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y8E0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990589">
        <property type="term" value="C:ATF4-CREB1 transcription factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035497">
        <property type="term" value="F:cAMP response element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14690">
        <property type="entry name" value="bZIP_CREB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000003">
        <property type="entry name" value="cAMP-responsive element modulator isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003102">
        <property type="entry name" value="CREB1-like_pKID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001630">
        <property type="entry name" value="Leuzip_CREB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879">
        <property type="entry name" value="CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50953"/>
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            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="35962" checksum="2F84A9CCBD59206C" modified="2020-06-17" version="1">MKMEAAVEVQQGAETAVTETESQQITPAQIATLAQVSMTTGHASATGPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPVQAVQVKHTQSRHISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPAVPRIEEEKAEEDSSAAAATPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAAAAQPGATILQYAQTSDGQQILVPSNQVVVQAASGDVQAYQIRAAPASTIAPGVVMASSPALPTQGATEEVTRKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAS6</accession>
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        <submittedName>
            <fullName evidence="7">Solute carrier family 22 member 21</fullName>
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        <name evidence="7" type="primary">LOC115432483</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <lineage>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="534" mass="59965" checksum="8DB6197A31986C5E" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YCK6</accession>
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        <submittedName>
            <fullName evidence="5">Immunoglobulin heavy variable 6-1</fullName>
        </submittedName>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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            <end position="113"/>
        </location>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="113" mass="12627" checksum="8BA8CE6A650FDACA" modified="2020-06-17" version="1">MHRKSHEFTFILTGVDGQTLTESEPAVKRPGQSHRLTCTTSGFTFSSYEMSWVRQAPGKGLEWIAFISTASSPIYYSSSVQGRFTISRDDSSSKLYLQMNSLKPEDTAVYNCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YGZ9</accession>
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        <submittedName>
            <fullName evidence="10">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
        </submittedName>
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    </organism>
    <reference evidence="10" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029996188.1">
        <property type="nucleotide sequence ID" value="XM_030140328.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A672YGZ9"/>
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        <property type="gene ID" value="ENSSORG00005002334.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115423490"/>
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    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="12"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="137"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="355"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="547" mass="62125" checksum="9C8025BB8B52502F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YL77</accession>
    <name>A0A672YL77_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized LOC115418961</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tmem176</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YL77"/>
    <dbReference type="Ensembl" id="ENSSORT00005005517.1">
        <property type="protein sequence ID" value="ENSSORP00005005360.1"/>
        <property type="gene ID" value="ENSSORG00005003182.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YL77"/>
    <dbReference type="OrthoDB" id="8936163at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF129">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005005360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="210" mass="23348" checksum="48E6059BD3E55C67" modified="2020-06-17" version="1">MAVAVTRDLTVQVLEDVNAAKLTDRQQALRAAIQRGEPKSLGVSQVMLGLMIIFYSIPLHLRSSSIIEYTEVIRLGVPWWSGLIFITAGAVAIFLDKHCNMKILQVCLIVSMLSVALSIVALIIYIVDFKKNPVSECIKQEYEDCDDKHYALNLSKGMKSALLLFTLTQTIISAIVCFLLYRQRRNFGQYATLNEGAPSSPTTVIPPELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLS4</accession>
    <name>A0A672YLS4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Protocadherin Fat 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">fat1a</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005005659.1">
        <property type="protein sequence ID" value="ENSSORP00005005473.1"/>
        <property type="gene ID" value="ENSSORG00005003264.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YLS4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000152">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000154">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000015">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000021">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000026">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000037">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000041">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000051">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000052">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000064">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Catalyzes the first step in the biosynthesis of chondroitin sulfate, heparan sulfate and dermatan sulfate proteoglycans, such as DCN. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>UDP-alpha-D-xylose + L-seryl-[protein] = 3-O-(beta-D-xylosyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12567"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
            <dbReference type="EC" id="2.4.2.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycan metabolism; chondroitin sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 14 family. XylT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.26" evidence="9"/>
    <dbReference type="RefSeq" id="XP_029998179.1">
        <property type="nucleotide sequence ID" value="XM_030142319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YQ99"/>
    <dbReference type="Ensembl" id="ENSSORT00005007026.1">
        <property type="protein sequence ID" value="ENSSORP00005006764.1"/>
        <property type="gene ID" value="ENSSORG00005003940.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115424922"/>
    <dbReference type="CTD" id="64132"/>
    <dbReference type="InParanoid" id="A0A672YQ99"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="UniPathway" id="UPA00755"/>
    <dbReference type="UniPathway" id="UPA00756"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030158">
        <property type="term" value="F:protein xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043538">
        <property type="entry name" value="XYLT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024448">
        <property type="entry name" value="XylT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46025:SF1">
        <property type="entry name" value="XYLOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46025">
        <property type="entry name" value="XYLOSYLTRANSFERASE OXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12529">
        <property type="entry name" value="Xylo_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="22" id="KW-0464">Manganese</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Xylosyltransferase C-terminal" evidence="28">
        <location>
            <begin position="532"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="43"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="849"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00011972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00039683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00041721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00045793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF12529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="876" mass="98823" checksum="8CEDBB2BD564191F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YWG6</accession>
    <name>A0A672YWG6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Homeobox protein Hox-B5a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hoxb5a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YWG6"/>
    <dbReference type="FunCoup" id="A0A672YWG6">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009195.1">
        <property type="protein sequence ID" value="ENSSORP00005008894.1"/>
        <property type="gene ID" value="ENSSORG00005004901.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YWG6"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000055">
        <property type="entry name" value="Homeobox protein Hox-A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="249"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="251"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="67"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="326" mass="34466" checksum="7031B1041B271C24" modified="2020-06-17" version="1">MSSYFVNSFSGRYPNGPDYQLLNYGASSGAMNGGTFRDSSTATMHHATGSYGYGYNGMDLTVSNRGGGVSGSSSAGGTGGHFGGGSVVGDSRGFGSPTTERGFRQPSSCSLAGADSLLPPGSGDTKLAAQSSSSPILDQPGSGNLSSPNLSTSSSGGGGGGTAPRFTELDDASPDTDEMHQQQQHNRDHGGHGGGGNVTPRTGHPQPGQKQDGGPAVSAPGSTTGNEGQPPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKDNKLKSMSLATSGSAFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z498</accession>
    <name>A0A672Z498_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">p2ry6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030008947.1">
        <property type="nucleotide sequence ID" value="XM_030153087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z498"/>
    <dbReference type="Ensembl" id="ENSSORT00005011979.1">
        <property type="protein sequence ID" value="ENSSORP00005011594.1"/>
        <property type="gene ID" value="ENSSORG00005006194.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432212"/>
    <dbReference type="CTD" id="5031"/>
    <dbReference type="InParanoid" id="A0A672Z498"/>
    <dbReference type="OrthoDB" id="9881476at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001621">
        <property type="term" value="F:G protein-coupled ADP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045029">
        <property type="term" value="F:G protein-coupled UDP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045030">
        <property type="term" value="F:G protein-coupled UTP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905835">
        <property type="term" value="P:cellular response to pyrimidine ribonucleotide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15379">
        <property type="entry name" value="7tmA_P2Y6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000371">
        <property type="entry name" value="P2Y3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF16">
        <property type="entry name" value="P2Y PURINOCEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01065">
        <property type="entry name" value="P2Y3PRNOCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="90"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="378" mass="43059" checksum="676A49100EB0D1E0" modified="2020-06-17" version="1">MSLHLLQSFKDVKMPHSVDFFPTETLISGTFSLDLLSQPTESYTADNFTLSVSNSNVSVPGIRSCTYKEDFKWILLPAVYTLVFLLGLPLNATVILKIWRTRPNLSRNNIYMLNLAIADFLYVMSLPLLIYNYASRDYWPFGELACKLVRFQFYSNLHGSILFLTCISVQRYVGICHPLAIWHKQGGRKLAWCICGGVWLVVALLCAPTFHFAATGIQRNRTVCYDLSPPNLSVQYYPYGMALTCLGFVLPFMGVMVCYCRMAHLLCRPVSYQGVSVATGEKRDKAVRMIIVVAVVFCISFLPFHLTKTMYLLVRSLPGVACETRNLFAIIYKSTRPFASMNSFLDPILFYFTQPRYRRSTRRFVLKITTLRDKGTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z584</accession>
    <name>A0A672Z584_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z584"/>
    <dbReference type="Ensembl" id="ENSSORT00005012848.1">
        <property type="protein sequence ID" value="ENSSORP00005012451.1"/>
        <property type="gene ID" value="ENSSORG00005006540.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z584"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051370">
        <property type="entry name" value="PPIase_Pin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657:SF4">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="WW" evidence="6">
        <location>
            <begin position="5"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="7">
        <location>
            <begin position="43"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="134" mass="15814" checksum="8128E49CA7ECE91A" modified="2020-06-17" version="1">RILASLLPSVWKETVWQYRVYFFNHITNCSQWERPVGDGRGEPEKVRCSHLLVKHNQSRRPSSWREQNITRSKDEALEFIQKYIEQIKSGEEKFESLASQFSDCSSAKTVETWDCLAEGRQGVLFLVRFVFFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z6U1</accession>
    <name>A0A672Z6U1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">braf</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z6U1"/>
    <dbReference type="FunCoup" id="A0A672Z6U1">
        <property type="interactions" value="1410"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012675.1">
        <property type="protein sequence ID" value="ENSSORP00005012278.1"/>
        <property type="gene ID" value="ENSSORG00005005159.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z6U1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17134">
        <property type="entry name" value="RBD_BRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14151">
        <property type="entry name" value="STKc_B-Raf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000015">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000024">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000004">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000036">
        <property type="entry name" value="RAF proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003116">
        <property type="entry name" value="RBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF240">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE B-RAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02196">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00455">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50898">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RBD" evidence="17">
        <location>
            <begin position="169"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="16">
        <location>
            <begin position="248"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="508"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="317"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="449"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="362"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="827" mass="91831" checksum="2858F2A1263B489F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z7M2</accession>
    <name>A0A672Z7M2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Isotocin-neurophysin IT 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">oxt</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the vasopressin/oxytocin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z7M2"/>
    <dbReference type="Ensembl" id="ENSSORT00005013247.1">
        <property type="protein sequence ID" value="ENSSORP00005012845.1"/>
        <property type="gene ID" value="ENSSORG00005006712.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z7M2"/>
    <dbReference type="OrthoDB" id="10056056at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005185">
        <property type="term" value="F:neurohypophyseal hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.9.10:FF:000001">
        <property type="entry name" value="oxytocin-neurophysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.9.10">
        <property type="entry name" value="Neurohypophysial hormone domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000981">
        <property type="entry name" value="Neurhyp_horm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036387">
        <property type="entry name" value="Neurhyp_horm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022423">
        <property type="entry name" value="Neurohypophysial_hormone_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681">
        <property type="entry name" value="NEUROPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681:SF13">
        <property type="entry name" value="VASOPRESSIN-NEUROPHYSIN 2-COPEPTIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00184">
        <property type="entry name" value="Hormone_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001815">
        <property type="entry name" value="Nonapeptide_hormone_precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00831">
        <property type="entry name" value="NEUROPHYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00003">
        <property type="entry name" value="NH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49606">
        <property type="entry name" value="Neurophysin II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00264">
        <property type="entry name" value="NEUROHYPOPHYS_HORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0027">Amidation</keyword>
    <keyword evidence="2" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0372">Hormone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025348340" evidence="8">
        <location>
            <begin position="20"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="20"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="41"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="59"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="106"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001815-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="156" mass="16334" checksum="EDA8C90CCBC5B69A" modified="2020-06-17" version="1">MTGVAVTVCLFFLMSVCSACYISNCPIGGKRSIMDAPLRKCMSCGPGDRGRCFGPSICCSEGFGCLMGSPETAHCVEENYLLTPCQAGGRPCGSEGGHCAASGLCCDSESCTTDQTCLTDEEGDDLTSQLEGSDTSDLILRLLHLAGHASPHRIHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z9H6</accession>
    <name>A0A672Z9H6_9TELE</name>
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        <submittedName>
            <fullName evidence="7">GTPase IMAP family member 9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115429471</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030004809.1">
        <property type="nucleotide sequence ID" value="XM_030148949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z9H6"/>
    <dbReference type="Ensembl" id="ENSSORT00005013550.1">
        <property type="protein sequence ID" value="ENSSORP00005013142.1"/>
        <property type="gene ID" value="ENSSORG00005006835.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429471"/>
    <dbReference type="InParanoid" id="A0A672Z9H6"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF112">
        <property type="entry name" value="SI:CH211-113E8.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025565788" evidence="5">
        <location>
            <begin position="24"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="36"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="199"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005013142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="329" mass="37145" checksum="1BA331F6A4B2EADB" modified="2020-06-17" version="1">MLQDKVWIAGCIVLLLITMFGQSAQCINQFKGSTEPRDLRLILVGKTGSGKSASGNTILGRKAFNTDISPESVTGTCQREEVEEDGRVISVIDSPGLFDTNKTEEHVKKAIEECVDLSLPGPHAFLLVISLKARFTQEEKAAVDWIQKNFGADADMYIILLFTHADLLGGKSVENFISESEDLRRLKNKCGGRYHSLINNSKRQNRLQVEELLEKIEEMVEENGGNPYTSEMYETAQKKLEESIKKRMEEEMRMKKEEERRIREQERKKEEIRRKMERIEEQERKANWCKAAAVGSIGLFGAGAYFASYSLMTLGAALGITEGYDCLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZBS0</accession>
    <name>A0A672ZBS0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized LOC115416429</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115416429</name>
        <name evidence="2" type="synonym">LOC115416439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029986060.1">
        <property type="nucleotide sequence ID" value="XM_030130200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZBS0"/>
    <dbReference type="Ensembl" id="ENSSORT00005014755.1">
        <property type="protein sequence ID" value="ENSSORP00005014333.1"/>
        <property type="gene ID" value="ENSSORG00005007339.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005014757.1">
        <property type="protein sequence ID" value="ENSSORP00005014335.1"/>
        <property type="gene ID" value="ENSSORG00005007341.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115416429"/>
    <dbReference type="OrthoDB" id="10024886at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22138">
        <property type="entry name" value="F-box_unchar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039588">
        <property type="entry name" value="FBXO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16008">
        <property type="entry name" value="F-BOX ONLY PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16008:SF6">
        <property type="entry name" value="SI:DKEY-12E7.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="31"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="382" mass="44763" checksum="77A2D246597B0E7F" modified="2020-06-17" version="1">MTTNICQTTIMTPRKRPLVPVSSRRKAADDYFPFNFLPVECQLHVLSFLNEVDKCSCALVCVSWSCLVRSWKLWRVADYSRRGVFHLGQEGLLVSNREFERWKSWVHHYTHHLISRRASLLTLKASFDLGDRCNKWSELLSHLLDHVHCRDLSHLDLNWTFTLLEPLDLRVHSSSSSHQDSITKMDQVTSFQELLSKLTHTCPRISKMRLHFDWSDVSVSLLTQFRQLRVLELKYFWVFKGVTPSTLQTLTQSLPQLKSLTLHILVPLRNLGISYTLESLSLEFLDVSPSRGLVFSRLKLPLLRELRAKKIVRGITLDRRTRLRIQSRWPCLYHVLREGTPKLQALNNERLLPTWREESYGELSAILEQSCYCVQHLDSWLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZD41</accession>
    <name>A0A672ZD41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nuclear pore complex protein Nup155</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">155 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nucleoporin Nup155</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">nup155</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Essential component of nuclear pore complex. Could be essessential for embryogenesis. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the non-repetitive/WGA-negative nucleoporin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005015203.1">
        <property type="protein sequence ID" value="ENSSORP00005014774.1"/>
        <property type="gene ID" value="ENSSORG00005007438.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZD41"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.450:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1880:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.440:FF:000001">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1880">
        <property type="entry name" value="Nucleoporin, helical C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.440">
        <property type="entry name" value="Nucleoporin, helical domain, central subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.450">
        <property type="entry name" value="Nucleoporin, helical domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004870">
        <property type="entry name" value="Nucleoporin_Nup155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042533">
        <property type="entry name" value="Nucleoporin_Nup155_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042537">
        <property type="entry name" value="Nucleoporin_Nup155_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042538">
        <property type="entry name" value="Nucleoporin_Nup155_C_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="20">
        <location>
            <begin position="79"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="19">
        <location>
            <begin position="651"/>
            <end position="1351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1375" mass="153455" checksum="8276E36E15D9EA89" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZDK7</accession>
    <name>A0A672ZDK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cell differentiation protein RQCD1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cnot9</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030013132.1">
        <property type="nucleotide sequence ID" value="XM_030157272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZDK7"/>
    <dbReference type="FunCoup" id="A0A672ZDK7">
        <property type="interactions" value="2013"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005015352.1">
        <property type="protein sequence ID" value="ENSSORP00005014919.1"/>
        <property type="gene ID" value="ENSSORG00005007590.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435079"/>
    <dbReference type="CTD" id="9125"/>
    <dbReference type="InParanoid" id="A0A672ZDK7"/>
    <dbReference type="OrthoDB" id="1183224at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000037">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005014919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="297" mass="33287" checksum="38DAC1C529F451A2" modified="2020-06-17" version="1">MLATGAAVTTALAQVDREKIYQWINELSSPETRENALLELSKKRESVPDLAPMLWHSCGTIAALLQEIVNIYPSINPPTLTAHQSNRVCNALALLQCVASHPETRSAFLAAHIPLFLYPFLHTVSKTRPFEYLRLTSLGVIGALVKTDEQEVINFLLTTEIIPLCLRIMESGSELSKTVATFILQKILLDDTGLAYICQTYERFSHVAMILGKMVLQLSKEPSARLLKHVVRCYLRLSDNSRAREALRQCLPDQLKDTTFAQVLKDDTTTKRWLAQLVKNLQEGQVTDPRGIPLPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZFP8</accession>
    <name>A0A672ZFP8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="4">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">pfas</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="4"/>
    <dbReference type="RefSeq" id="XP_029988143.1">
        <property type="nucleotide sequence ID" value="XM_030132283.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A672ZFP8">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005016321.1">
        <property type="protein sequence ID" value="ENSSORP00005015825.1"/>
        <property type="gene ID" value="ENSSORG00005006216.1"/>
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    <sequence length="1316" mass="143128" checksum="BD0E19AF8825D49D" modified="2020-06-17" version="1"> </sequence>
</entry>
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    <accession>A0A672ZHZ6</accession>
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</entry>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZIX2"/>
    <dbReference type="Ensembl" id="ENSSORT00005017240.1">
        <property type="protein sequence ID" value="ENSSORP00005016729.1"/>
        <property type="gene ID" value="ENSSORG00005008460.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZIX2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06376">
        <property type="entry name" value="PBP1_mGluR_groupIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000196">
        <property type="entry name" value="Glutamate metabotropic receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000009">
        <property type="entry name" value="Glutamate receptor, metabotropic 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000001">
        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000176">
        <property type="entry name" value="metabotropic glutamate receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001786">
        <property type="entry name" value="GPCR_3_mGluR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000162">
        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01054">
        <property type="entry name" value="MTABOTROPC4R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="582"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="620"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="651"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="688"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="779"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="821"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="15">
        <location>
            <begin position="582"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="870"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="896" mass="99782" checksum="DA08B3130A7DDEB4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQ05</accession>
    <name>A0A672ZQ05_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:myosin light chain binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16435">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 6 SPATA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="166" mass="19321" checksum="49E6B4AC501A4B1A" modified="2020-06-17" version="1">QNIIKWHFNVDRKHTVSGKCSYQRPTVSSRTRALSPYTHRRMCQLSEDARQRLSHLRLGPHLFRKHSESQPPFLVLSSCSNMMDSNGRQCDKVLFSCFLPSNKTFSFVLHCNTSVSVSCNHLYRRSNNYNCQNCDYNQFIIPLPSDLTLKTHCHCVKVDTFLYKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQE6</accession>
    <name>A0A672ZQE6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Microprocessor complex subunit DGCR8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">dgcr8</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization. Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region). Interacts with ILF3, NCL and DROSHA. Interacts with CPSF3 and ISY1; this interaction is in an RNA dependent manner. Interacts with PUS10; interaction promotes pri-miRNAs processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZQE6"/>
    <dbReference type="FunCoup" id="A0A672ZQE6">
        <property type="interactions" value="2431"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005019165.1">
        <property type="protein sequence ID" value="ENSSORP00005018617.1"/>
        <property type="gene ID" value="ENSSORG00005008793.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZQE6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070877">
        <property type="term" value="C:microprocessor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070878">
        <property type="term" value="F:primary miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19867">
        <property type="entry name" value="DSRM_DGCR8_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19868">
        <property type="entry name" value="DSRM_DGCR8_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000018">
        <property type="entry name" value="DGCR8 microprocessor complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000023">
        <property type="entry name" value="DGCR8, microprocessor complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000021">
        <property type="entry name" value="Microprocessor complex subunit DGCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.590:FF:000001">
        <property type="entry name" value="microprocessor complex subunit DGCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040375">
        <property type="entry name" value="DGCR8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13482:SF3">
        <property type="entry name" value="MICROPROCESSOR COMPLEX SUBUNIT DGCR8"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR13482">
        <property type="entry name" value="MICRORNA PROCESSOR COMPLEX SUBUNIT DGCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="WW" evidence="16">
        <location>
            <begin position="292"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="17">
        <location>
            <begin position="521"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="127"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="352"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="368"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="412"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="783" mass="87661" checksum="29DC6DA2D7B57A80" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZS06</accession>
    <name>A0A672ZS06_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Low density lipoprotein receptor-related protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">lrp3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <location evidence="10">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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    <dbReference type="FunCoup" id="A0A672ZS06">
        <property type="interactions" value="802"/>
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    <dbReference type="Ensembl" id="ENSSORT00005020229.1">
        <property type="protein sequence ID" value="ENSSORP00005019669.1"/>
        <property type="gene ID" value="ENSSORG00005009643.1"/>
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    <dbReference type="InParanoid" id="A0A672ZS06"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000050">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF22">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
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    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025644563" evidence="13">
        <location>
            <begin position="18"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="31"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="632"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="717"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="633"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="749"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="762"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="170"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="392"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="810" mass="88694" checksum="2854677881B89955" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZSY2</accession>
    <name>A0A672ZSY2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein L13a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZSY2"/>
    <dbReference type="Ensembl" id="ENSSORT00005020835.1">
        <property type="protein sequence ID" value="ENSSORP00005020266.1"/>
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    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZSY2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="term" value="P:negative regulation of translation"/>
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        <property type="entry name" value="60S ribosomal protein L13a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
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        <property type="entry name" value="Ribosomal_uL13_sf"/>
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    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
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        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
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        <property type="entry name" value="Ribosomal protein L13"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="141" mass="15943" checksum="F941D3ECEE0689FC" modified="2020-06-17" version="1">MNTNPSRGPYHFRAPSRNLLRTVRGELYKDSCSGEAEGVDGIPPPYDKGIINDSRMVVPAALKIVRLKPSRKFALLGRLAHEVGWKYQAITATLEEKRKEKAKLRYAKKKTVMKLTKQAEKNVEAKIAKYTDVLKQYGVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZT56</accession>
    <name>A0A672ZT56_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein ref(2)P</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Refractory to sigma P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">sqstm1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Required for selective autophagy activation by ubiquitinated proteins. Implicated in sigma rhabdovirus multiplication and necessary for male fertility. Involved in activating transcription of Drs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with aPKC and Traf6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030001795.1">
        <property type="nucleotide sequence ID" value="XM_030145935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZT56"/>
    <dbReference type="FunCoup" id="A0A672ZT56">
        <property type="interactions" value="719"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020041.1">
        <property type="protein sequence ID" value="ENSSORP00005019483.1"/>
        <property type="gene ID" value="ENSSORG00005009576.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427396"/>
    <dbReference type="CTD" id="8878"/>
    <dbReference type="InParanoid" id="A0A672ZT56"/>
    <dbReference type="OrthoDB" id="441278at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016235">
        <property type="term" value="C:aggresome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044753">
        <property type="term" value="C:amphisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd06402">
        <property type="entry name" value="PB1_p62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14320">
        <property type="entry name" value="UBA_SQSTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000016">
        <property type="entry name" value="Refractory to sigma P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000034">
        <property type="entry name" value="Sequestosome 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000169">
        <property type="entry name" value="Sequestosome 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052260">
        <property type="entry name" value="Autophagy_Rcpt_SigReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034866">
        <property type="entry name" value="PB1_p62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033741">
        <property type="entry name" value="SQSTM_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15090">
        <property type="entry name" value="SEQUESTOSOME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15090:SF0">
        <property type="entry name" value="SEQUESTOSOME-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16577">
        <property type="entry name" value="UBA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PB1" evidence="18">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="17">
        <location>
            <begin position="131"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="203"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="273"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00054138"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00062450"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00071657"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081379"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="428" mass="46660" checksum="B0B35541E82C9210" modified="2020-06-17" version="1">MSVTVKAYLLGKDEAVKEIRRFTVDQEVTCSFEYLSRKTATVFSHLTSGAFNTYYRDEDGDLVAFSSDDELMMGLACVKDATFRLYIKEKKEHRRDFPLHAFPPFTFGPPPPHGPPPHGPPPHHAPPPAVHPNVTCDGCNGAVMGTRFKCSVCPNYDLCSSCQAQGIHTEHALLPIWHPLQWFPRGKWMKRMRHCMWNLNQEQNQNQDQDRPGASSSSTAPPPPSTAAPGPNGSAPSASQANVEFLKNIGEGVAAMLSPLGIDVDIDVEHEGQRTKVTPPTQSDGDVEMEGAEGGGGASQEGGAKEGPAVSRDSDEEWTDLSCREVDPSTGELQSLQDQDQDQVQVQDQDRGPPSAQQGPSGLREAALYPHLPQEANPRLVESLSQMLSMGFTDEGGWLTRLLQAKGFDIGAALDAIQYAKQPRPQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZU84</accession>
    <name>A0A672ZU84_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">MICAL-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">micall2b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
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    <sequence length="791" mass="87369" checksum="A8D9518B21A96305" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZUP5</accession>
    <name>A0A672ZUP5_9TELE</name>
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        <submittedName>
            <fullName evidence="20">Lipase member H-like</fullName>
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        <name evidence="20" type="primary">lipia</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="14">Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + hexadecanoate + H(+)</text>
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        <physiologicalReaction direction="left-to-right" evidence="13">
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        <subcellularLocation>
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            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the AB hydrolase superfamily. Lipase family.</text>
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        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
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    </dbReference>
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        <property type="entry name" value="TAGLIPASE"/>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025685956" evidence="18">
        <location>
            <begin position="21"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="19">
        <location>
            <begin position="39"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="252"/>
        </location>
    </feature>
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        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
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            <position position="198"/>
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        <ligand>
            <name>Ca(2+)</name>
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        <location>
            <position position="201"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="451" mass="50929" checksum="DF9A9155B6D3DDCE" modified="2020-06-17" version="1">MFLWQYLAPFLLITIQICRAQTCEDFTDLDLSHAFIGTSLRIKLLLYTRENATCGILLSHTNPTAHPQFKLSRPTTFVIHGYRPTGSPPNWIHNITDMLLSRDDMNVIVVDWNHGAANVNYMKAVENTHKAADNLTALMKKLQEHGVSLSSVHLIGVSLGAHISGFIGASLNSTIGRITALDPAGPLFTGKPPEDRLDPTDAQFVDVLHTDIDALGFREPLGHIDFYANGGTDQPGCPKTIFSGGSYFKCDHQRSVFLYLDSEEQKCASRAYPCTSYSDYMDGQCLNCDRFGTAGCPVFGYDITKWKDVLLNLGQTKTYFSTNKKSPFCMTNYVVDIVTWNQEIRWGYITIKLHSGSKEAVATIDHKVSKFKKFTQTKLLAQFDKDVPSVKKISLKFTTGNVLKPKYKLRVLRMRLTPIERKERPLCRYDILLPENEEVTFRPIPCEEYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZW19</accession>
    <name>A0A672ZW19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZW19"/>
    <dbReference type="Ensembl" id="ENSSORT00005021199.1">
        <property type="protein sequence ID" value="ENSSORP00005020622.1"/>
        <property type="gene ID" value="ENSSORG00005010025.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZW19"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="2">
        <location>
            <begin position="46"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="136" mass="15540" checksum="3F40D34AD96849BA" modified="2020-06-17" version="1">MAQQEEPLYDFPEPSQPSKFLGHQRGWGSLKSISVLDRLLLTVPVWLQLSINPATALHILQREPPGTFLVRKSRTSQKNVLCVRLADDSVPSFVQQFGIREEQSSKRLNSPKLSLVLRCHFDVRPEVFTEVLTQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZY44</accession>
    <name>A0A672ZY44_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PiggyBac transposable element-derived protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC115434577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZY44"/>
    <dbReference type="Ensembl" id="ENSSORT00005021840.1">
        <property type="protein sequence ID" value="ENSSORP00005021203.1"/>
        <property type="gene ID" value="ENSSORG00005010352.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZY44"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599:SF3">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="2">
        <location>
            <begin position="127"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="605" mass="69117" checksum="1EBFD98AADB27576" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A043</accession>
    <name>A0A673A043_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">sema4c</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029999252.1">
        <property type="nucleotide sequence ID" value="XM_030143392.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029999253.1">
        <property type="nucleotide sequence ID" value="XM_030143393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A043"/>
    <dbReference type="Ensembl" id="ENSSORT00005023279.1">
        <property type="protein sequence ID" value="ENSSORP00005022614.1"/>
        <property type="gene ID" value="ENSSORG00005010995.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425698"/>
    <dbReference type="CTD" id="54910"/>
    <dbReference type="InParanoid" id="A0A673A043"/>
    <dbReference type="OrthoDB" id="9934005at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11258">
        <property type="entry name" value="Sema_4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001170">
        <property type="entry name" value="Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (Semaphorin) 3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000033">
        <property type="entry name" value="Semaphorin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000018">
        <property type="entry name" value="semaphorin-4C-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF16">
        <property type="entry name" value="SEMAPHORIN-4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025415994" description="Sema domain-containing protein" evidence="17">
        <location>
            <begin position="28"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="678"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="19">
        <location>
            <begin position="35"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="564"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="818"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="823"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="868" mass="96278" checksum="73104FC5826C1FBF" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A050</accession>
    <name>A0A673A050_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC115423365</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A050"/>
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    </dbReference>
    <dbReference type="InParanoid" id="A0A673A050"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="76"/>
            <end position="208"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="952" mass="106736" checksum="FE0798495921FF55" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A532</accession>
    <name>A0A673A532_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A532"/>
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        <property type="gene ID" value="ENSSORG00005011832.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A532"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
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    </dbReference>
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        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="45"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="163" mass="18183" checksum="8D0405B87C7C85EA" modified="2020-06-17" version="1">IFISPSVYTVACTKHHTGILHCIDFFFFLTSSFAGMSCETGWSKFGMNCYYDSVTELDWVHSRLVCLSMGADLVSINTAEEQAFINGLLSPQVNAWIGLNDIHSEGKWKWVDGTAVTTTFWGPGQPNSYNGTNQDCGELVENSLMGQWNDEDCSAENIWICEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5N0</accession>
    <name>A0A673A5N0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 21">Phosphatidate cytidylyltransferase, mitochondrial</fullName>
            <ecNumber evidence="7 21">2.7.7.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19 21">CDP-diacylglycerol synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20 21">Mitochondrial translocator assembly and maintenance protein 41 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">tamm41</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 21">Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 21">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 21">Mitochondrion inner membrane</location>
            <topology evidence="3 21">Peripheral membrane protein</topology>
            <orientation evidence="3 21">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 21">Belongs to the TAM41 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="7 21"/>
    <dbReference type="AlphaFoldDB" id="A0A673A5N0"/>
    <dbReference type="FunCoup" id="A0A673A5N0">
        <property type="interactions" value="1351"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024652.1">
        <property type="protein sequence ID" value="ENSSORP00005023947.1"/>
        <property type="gene ID" value="ENSSORG00005011560.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A5N0"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015222">
        <property type="entry name" value="Tam41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619:SF0">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09139">
        <property type="entry name" value="Tam41_Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028840">
        <property type="entry name" value="Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 21" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 21" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17 21" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="18 21" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 21" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR028840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="344" mass="38396" checksum="9CDA66281AB8D926" modified="2020-06-17" version="1">MTLPALHSTGVLYRRILAQFPQDISLAFAYGSGVFKQQGTTQGQMDKNMLDFVFAVDDPVTWHTMNLLQNRKHYSILKLLGPTTISSIQNEYGAAVYYNTLVPVDGRVIKYGVISTDSLINDLMHWKTLYVAGRLHKPVSILELPGENGKLRAALVANLKSAVTASFLMLPESFSEEDLFLQIAGLSYAGDFRMVIGEDKSKVTNIVKDNIQHFRILYSNILRDCPQVVYKPQQGKLEVDKSPEGQFVQLMALPRTLQQRITKLVDPPGKNRDVEEILLQVAQDPDCGAVVQQGISSIVKSSSITQSIKGIGTAGLWKTVSYSSKKLMKMWRGWRRKPSVSQMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6B8</accession>
    <name>A0A673A6B8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">DNA-binding protein SMUBP-2</fullName>
            <ecNumber evidence="5">3.6.4.12</ecNumber>
            <ecNumber evidence="6">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">ATP-dependent helicase IGHMBP2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="primary">ighmbp2</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">5' to 3' helicase that unwinds RNA and DNA duplexes in an ATP-dependent reaction. Specific to 5'-phosphorylated single-stranded guanine-rich sequences. May play a role in RNA metabolism, ribosome biogenesis or initiation of translation. May play a role in regulation of transcription. Interacts with tRNA-Tyr.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="28">Homooligomer. Interacts with RUVBL1. Interacts with RUVBL2. Interacts with GTF3C1. Interacts with ABT1. Interacts with ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="5"/>
    <dbReference type="EC" id="3.6.4.13" evidence="6"/>
    <dbReference type="RefSeq" id="XP_029991646.1">
        <property type="nucleotide sequence ID" value="XM_030135786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673A6B8"/>
    <dbReference type="Ensembl" id="ENSSORT00005024909.1">
        <property type="protein sequence ID" value="ENSSORP00005024198.1"/>
        <property type="gene ID" value="ENSSORG00005011627.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115420506"/>
    <dbReference type="CTD" id="3508"/>
    <dbReference type="InParanoid" id="A0A673A6B8"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18044">
        <property type="entry name" value="DEXXQc_SMUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02641">
        <property type="entry name" value="R3H_Smubp-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001171">
        <property type="entry name" value="DNA-binding protein SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001146">
        <property type="entry name" value="DNA-binding protein SMUBP-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.270:FF:000001">
        <property type="entry name" value="Immunoglobulin mu DNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000002">
        <property type="entry name" value="Immunoglobulin mu DNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000002">
        <property type="entry name" value="Immunoglobulin mu DNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034072">
        <property type="entry name" value="R3H_Smubp-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048761">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00376">
        <property type="entry name" value="IGHMBP2 family helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21138">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="ZF_AN1"/>
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    </dbReference>
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    <keyword evidence="20" id="KW-0010">Activator</keyword>
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    <keyword evidence="8" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
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        <location>
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            <end position="779"/>
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        <location>
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            <end position="941"/>
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        </location>
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            <end position="814"/>
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            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="974"/>
            <end position="995"/>
        </location>
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        <location>
            <begin position="762"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="32">
        <location>
            <begin position="783"/>
            <end position="801"/>
        </location>
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        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
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        <location>
            <begin position="840"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="32">
        <location>
            <begin position="856"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="32">
        <location>
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            <end position="983"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022555"/>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="995" mass="109989" checksum="157ADB6982F8BBD3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A7C2</accession>
    <name>A0A673A7C2_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">Cellular retinoic acid-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cellular retinoic acid-binding protein I</fullName>
        </alternativeName>
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        <name evidence="7" type="primary">rbp5</name>
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        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Apogonoidei</taxon>
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            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.</text>
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    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="137" mass="15696" checksum="4A9D4BE20AE8279C" modified="2020-06-17" version="1">MSKPNYTGVYHMVEQQNMDEYLGALDINFALRKIVCLLKPTKDITHDPATGAMKIRTITTFKNFNMDFNIGEEFTEDLGPVDGRTCQTTVSWEGDKLVCVQRGEKEGRGWTHWLEGDKLHLEMRVQGVVAKQVFKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A7J4</accession>
    <name>A0A673A7J4_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Transmembrane protein 136-like</fullName>
        </submittedName>
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        <name evidence="8" type="primary">tlcd5a</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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        </lineage>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A7J4"/>
    <dbReference type="FunCoup" id="A0A673A7J4">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005026022.1">
        <property type="protein sequence ID" value="ENSSORP00005025266.1"/>
        <property type="gene ID" value="ENSSORG00005012150.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="506011at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042512">
        <property type="entry name" value="TLCD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898:SF4">
        <property type="entry name" value="TLC DOMAIN-CONTAINING 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="28"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="242" mass="27474" checksum="3656DCCFF89EB107" modified="2020-06-17" version="1">MAVLVFCALLCLSAWVSLYFFLCNINGSRSYEWNCRLVTLLHGILAICITAYIGYIDGPWPFDHPGTPNTTLQISAMVLSLGYFFFDMAWCVYFRTEGPVMLAHHTMSILGILLTLWLGESGIESCAVLFGSEITNPLLQARWFLKQTGRYGTWLGNVVDVLFVLLFVLMRIFVGGTMLYCELISPRPRFFIKCGGVAMYALSWVFMVDIVRFAIRKSKSWHIQPKEQQESLAANGYDNKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABP1</accession>
    <name>A0A673ABP1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115415147</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ABP1"/>
    <dbReference type="Ensembl" id="ENSSORT00005027468.1">
        <property type="protein sequence ID" value="ENSSORP00005026686.1"/>
        <property type="gene ID" value="ENSSORG00005012783.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABP1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="14">
        <location>
            <begin position="27"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="15">
        <location>
            <begin position="199"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="560"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="11">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="659" mass="75349" checksum="EC5A82896D086A27" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ABX7</accession>
    <name>A0A673ABX7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Voltage-dependent L-type calcium channel subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC115419534</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005026806.1">
        <property type="protein sequence ID" value="ENSSORP00005026037.1"/>
        <property type="gene ID" value="ENSSORG00005012511.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ABX7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000007">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000009">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000021">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000001">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000006">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000010">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000040">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031649">
        <property type="entry name" value="GPHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014873">
        <property type="entry name" value="VDCC_a1su_IQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005450">
        <property type="entry name" value="VDCC_L_a1ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005446">
        <property type="entry name" value="VDCC_L_a1su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002077">
        <property type="entry name" value="VDCCAlpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF9">
        <property type="entry name" value="VOLTAGE-DEPENDENT L-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08763">
        <property type="entry name" value="Ca_chan_IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16905">
        <property type="entry name" value="GPHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00167">
        <property type="entry name" value="CACHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01630">
        <property type="entry name" value="LVDCCALPHA1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01634">
        <property type="entry name" value="LVDCCALPHA1S"/>
    </dbReference>
    <dbReference type="SMART" id="SM01062">
        <property type="entry name" value="Ca_chan_IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 19" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AC13</accession>
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        <recommendedName>
            <fullName evidence="16">Zinc finger protein 281</fullName>
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        <name type="scientific">Sphaeramia orbicularis</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="15">Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.</text>
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        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
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    </evidence>
    <sequence length="801" mass="86563" checksum="94CF2607C3E95186" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACF9</accession>
    <name>A0A673ACF9_9TELE</name>
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        <submittedName>
            <fullName evidence="12">Plexin domain-containing protein 1-like</fullName>
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        <name evidence="12" type="primary">LOC115410779</name>
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    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029978433.1">
        <property type="nucleotide sequence ID" value="XM_030122573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ACF9"/>
    <dbReference type="FunCoup" id="A0A673ACF9">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005027720.1">
        <property type="protein sequence ID" value="ENSSORP00005026936.1"/>
        <property type="gene ID" value="ENSSORG00005012897.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115410779"/>
    <dbReference type="InParanoid" id="A0A673ACF9"/>
    <dbReference type="OrthoDB" id="6285106at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031152">
        <property type="entry name" value="PLXDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055:SF10">
        <property type="entry name" value="PLEXIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055">
        <property type="entry name" value="TUMOR ENDOTHELIAL MARKER 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025484947" evidence="10">
        <location>
            <begin position="22"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="11">
        <location>
            <begin position="295"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="358"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="478" mass="53708" checksum="82E8F935F70D1DA6" modified="2020-06-17" version="1">MGLCAVLLIYLSQAELGRVWAQEQGDALSETLLQTDVAPSSVHRVRKDQETLHKHREPRNTVGGVDISSLPNNVTKILEDSQRYYRWQSFGPTDRRTEDLWVDLSGPAKSQVRIHGILSNAHRQAARVALSFDFPFYGHYLRQIIVATGGFIYMGDVTHRMLTATQYIAPLMANFDPSFSLNSTVRYTDNGNLFVVQWDKVRLKDREAEGPFTFQTALHKNGTIVFNYREIPLLVENINSTEHPVKVGLSDAFMAFLPSQLSDAKQWTIYEYHRVEIDTTKIVNRSAFEFTPLPTCLQHTSCELCLTSNLTTGCGWCNTLQRCSDGIDRHRQEWLDYNCPEEAKGTCEDYNPVLPEGSMGPFNHSSPGPTPSSSETDNQPVTTDLHTGPPNHTGITENTAIIAGVVAALVLLVALTLLAVYYINTHPTVAPPFYLMQRRTNNYWPSMKFRNQGCHSSYAEVELGGHEKEGFIEAEQCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ACZ7</accession>
    <name>A0A673ACZ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC115436923</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673ACZ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005027879.1">
        <property type="protein sequence ID" value="ENSSORP00005027094.1"/>
        <property type="gene ID" value="ENSSORG00005012870.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ACZ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05066">
        <property type="entry name" value="PTKc_EphR_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000019">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000045">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000059">
        <property type="entry name" value="Ephrin type-A receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF18">
        <property type="entry name" value="EPHRIN TYPE-A RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 21 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 21 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="28">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="27">
        <location>
            <begin position="306"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="27">
        <location>
            <begin position="421"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="594"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="719"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="600"/>
            <end position="608"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="626"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="51"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="965" mass="107151" checksum="FFF6A072BC6B82C2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AIX1</accession>
    <name>A0A673AIX1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Amyloid beta (A4) precursor-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">aplp1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AIX1"/>
    <dbReference type="FunCoup" id="A0A673AIX1">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030050.1">
        <property type="protein sequence ID" value="ENSSORP00005029219.1"/>
        <property type="gene ID" value="ENSSORG00005013054.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AIX1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031694">
        <property type="term" value="F:alpha-2A adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031695">
        <property type="term" value="F:alpha-2B adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031696">
        <property type="term" value="F:alpha-2C adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21708">
        <property type="entry name" value="JMTM_APLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF13">
        <property type="entry name" value="AMYLOID BETA PRECURSOR LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00203">
        <property type="entry name" value="AMYLOIDA4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="13">
        <location>
            <begin position="24"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="14">
        <location>
            <begin position="310"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="10">
        <location>
            <begin position="24"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="10">
        <location>
            <begin position="127"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="186"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="205"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="69"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="94"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="140"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="684" mass="78656" checksum="B7178B3379567917" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AJB2</accession>
    <name>A0A673AJB2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Si:dkey-96n2.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AJB2"/>
    <dbReference type="Ensembl" id="ENSSORT00005030643.1">
        <property type="protein sequence ID" value="ENSSORP00005029805.1"/>
        <property type="gene ID" value="ENSSORG00005014244.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AJB2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF105">
        <property type="entry name" value="URACIL NUCLEOTIDE_CYSTEINYL LEUKOTRIENE RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="30"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="317" mass="36501" checksum="FDCA16ED6E29B449" modified="2020-06-17" version="1">MNISEEGFIHRENIVFATFYILIFIFAVPGNALALWAFFHQSSTSPSKVFLRHLSVADISYILILIMRIVYHLFDSYWPFGHVLCKVAGFLFYLNMYCSMYLMSFISLDRFLAVVLPIRSQSIRKPLYANVIVGILWMTIIVSMCPILIKEEAINNTTGICNKLYLEDTSSTALVSTIVAFVLPLTTIVISYILILWKLRTVKKQGNRAVRDKAIKMIILILMNFLIAFVPYHVNRVIYITQGHENTATDETLGRVNRITSALTCVSGVLDPVMYFFLNRAYRDKLHQLIQHQQGNSRCFTQDIEIKETKRKTFKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AM35</accession>
    <name>A0A673AM35_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NLR family CARD domain-containing protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115435417</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030013669.1">
        <property type="nucleotide sequence ID" value="XM_030157809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AM35"/>
    <dbReference type="Ensembl" id="ENSSORT00005030512.1">
        <property type="protein sequence ID" value="ENSSORP00005029678.1"/>
        <property type="gene ID" value="ENSSORG00005014170.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435417"/>
    <dbReference type="InParanoid" id="A0A673AM35"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="334"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="187"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1056" mass="120731" checksum="EA8782687169B9B5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMJ1</accession>
    <name>A0A673AMJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nub1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029979508.1">
        <property type="nucleotide sequence ID" value="XM_030123648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AMJ1"/>
    <dbReference type="Ensembl" id="ENSSORT00005031434.1">
        <property type="protein sequence ID" value="ENSSORP00005030576.1"/>
        <property type="gene ID" value="ENSSORG00005014587.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411487"/>
    <dbReference type="CTD" id="51667"/>
    <dbReference type="InParanoid" id="A0A673AMJ1"/>
    <dbReference type="OrthoDB" id="434245at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000058">
        <property type="term" value="P:regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14291">
        <property type="entry name" value="UBA1_NUB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039749">
        <property type="entry name" value="NUB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041207">
        <property type="entry name" value="NUB1_ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12948:SF3">
        <property type="entry name" value="NEDD8 ULTIMATE BUSTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12948">
        <property type="entry name" value="NEDD8 ULTIMATE BUSTER-1 BS4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18037">
        <property type="entry name" value="Ubiquitin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="367"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="418"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="473"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="525"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="397"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="597" mass="67754" checksum="6321CFB931CCF539" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AN03</accession>
    <name>A0A673AN03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">mul2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673AN03"/>
    <dbReference type="FunCoup" id="A0A673AN03">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031608.1">
        <property type="protein sequence ID" value="ENSSORP00005030746.1"/>
        <property type="gene ID" value="ENSSORG00005014658.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AN03"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051652">
        <property type="entry name" value="MDM2_MDM4_MUL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022170">
        <property type="entry name" value="MUL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12183">
        <property type="entry name" value="MITOCHONDRIAL UBIQUITIN LIGASE ACTIVATOR OF NFKB 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12183:SF6">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12483">
        <property type="entry name" value="GIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="308"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="357" mass="40220" checksum="F22AEC73C639318D" modified="2020-06-17" version="1">MDEFPVSLSEVVCLGASFALSGICYYLYRKSRTTVDMLHDAPHFNIDGKLKDILKVTPGACLKYAVIEGSVQPLEEPLMSHYQKESVGVLQKFMLREHRRVWNGLSRTWTDSERVLHERVNIVPFGLVGPDETTVEVLCPLQASGVHMEMTHQKFHQATSSLGDLLGQYLSGEKLKGQLETEDMLKVGTILTGVGELVLDTDGTLSLRPPSNGADYILSITDFDTLLGEHKSTASWWKTLAIVFALAGAAVVLWVGRSYYNQLKVRWELERERRQFERLRDEGRRVEAAATGPDAPQDAAEERIENTCVICLTQQRDCILLDCGHVCCCYQCYQALPQPRCPICRQMINRVVPLYHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANG6</accession>
    <name>A0A673ANG6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IRF tryptophan pentad repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ANG6"/>
    <dbReference type="Ensembl" id="ENSSORT00005031661.1">
        <property type="protein sequence ID" value="ENSSORP00005030799.1"/>
        <property type="gene ID" value="ENSSORG00005014457.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ANG6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019817">
        <property type="entry name" value="Interferon_reg_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001346">
        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019471">
        <property type="entry name" value="Interferon_reg_factor-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF1">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01243">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00601">
        <property type="entry name" value="IRF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="7">
        <location>
            <begin position="5"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="415" mass="46402" checksum="78351D73AC4F5E66" modified="2020-06-17" version="1">MAQSRPLLIPWLRTKLDSGNYPGVNWTNPERTEFCIPWKHALRQDSTNTDVLIFKAWAEVTGNGRAQGDASVWKRNFRSALRAKGFKMIADNKNDAANPHKVFRWPDESRSGANSQDQNDPDLFEDFPLPVQESLLQVVPDFEGCIYLPEDSLFSNDAAPKQDILEECLKGLNIGPETGRLDTQLEPPPEQQQLQNQVVIGGHVLRGQQQYPVPFEGAVGEAGLPEQPAHPMEGAVGGACSDHAEQFLHTMSQTNDGDHFKTQFKVIVYYRGVKVSEQLVENESGVRIVYSLDQESGLSLISLPTPGDMHDKPTANLTPSHPGQADGLDVGYRQSFLSMSKFDQEQTPSRKFIQVDRPPCSLFFCLGEKWPDPDNRPWWFLTSMELLKNLAVANGASSLQSVDLQMSLEEMMEMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANT1</accession>
    <name>A0A673ANT1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EGF-CFC (Cripto-1/FRL1/Cryptic) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ANT1"/>
    <dbReference type="Ensembl" id="ENSSORT00005031898.1">
        <property type="protein sequence ID" value="ENSSORP00005031031.1"/>
        <property type="gene ID" value="ENSSORG00005014804.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ANT1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000421">
        <property type="entry name" value="Teratocarcinoma-derived growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019011">
        <property type="entry name" value="Cryptic/Cripto_CFC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09443">
        <property type="entry name" value="CFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="56"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="166" mass="18652" checksum="6881D7A23C9AFC12" modified="2020-06-17" version="1">CFSFVANCFSFVANWFLFVANCFCLLTVFLLLLTGSCLLLTVFVWLLTVFFFWLLLTGSCSDQSRGCCKNGGTCILGSFCACPPFFTGRSCEYDQRIRRCGAVPHGSWVQKGCSYCRCGYGVLHCFPHVFNKDCGKTRPRAPVPCGPAHRALFWPLPRPQLKVEWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AP21</accession>
    <name>A0A673AP21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Multifunctional methyltransferase subunit TRM112-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 112 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trmt112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRM112 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030001948.1">
        <property type="nucleotide sequence ID" value="XM_030146088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AP21"/>
    <dbReference type="FunCoup" id="A0A673AP21">
        <property type="interactions" value="1509"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005031875.1">
        <property type="protein sequence ID" value="ENSSORP00005031009.1"/>
        <property type="gene ID" value="ENSSORG00005014789.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427510"/>
    <dbReference type="CTD" id="51504"/>
    <dbReference type="InParanoid" id="A0A673AP21"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21089">
        <property type="entry name" value="Trm112-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000015">
        <property type="entry name" value="Multifunctional methyltransferase subunit TRM112-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="123" mass="14014" checksum="33EC009FFB9249AD" modified="2020-06-17" version="1">MKLLTHNMLTSHVKGVIKGYPLLIKATEVKVNEVEFNPQFVSRMIPKLEWSALVQAAEELGQRQDLPSDLVPDYETNEEFLKKVHRVLLEVEVVEGCLQCPESGREFPISRGIPNMLLNEDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQ64</accession>
    <name>A0A673AQ64_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein regulatory complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Growth arrest-specific protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115417257</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DRC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AQ64"/>
    <dbReference type="FunCoup" id="A0A673AQ64">
        <property type="interactions" value="349"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032694.1">
        <property type="protein sequence ID" value="ENSSORP00005031810.1"/>
        <property type="gene ID" value="ENSSORG00005015135.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQ64"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039308">
        <property type="entry name" value="GAS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025593">
        <property type="entry name" value="GAS8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31543">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31543:SF0">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13851">
        <property type="entry name" value="GAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Growth arrest-specific protein 8" evidence="15">
        <location>
            <begin position="223"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="301"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="414" mass="49207" checksum="5EA411D8039EF848" modified="2020-06-17" version="1">QPPKNKGSSKKPPKVKTPTLIDGLTKDEMTKEQLEEHIARLREELDREREERNYFQLERDKIQTFWDITERQLKEAKAELTNLDRDVDEDQKHHQIETKVYKQKMKHLLCEHQNTVCELSADGLASSEVMQTKKTELENEIHDKIMSIRVDIQERDNESLVKETEKKHEDEIHKTRESLEKKFREVEDKYKRKLELLREELDNKRKSEICEREEHWNKHIATIMEVHKKALNKAREFINSTEKELDLNISLKKEIENENMKLKQKEKDLTSVLQDNKHLAECLSKVKEETFQIEKQMKHSMSKLRNNERFKERQLNNLMREHELLKNKFREIQQEGDELSKSFTRSFQKLQQKTDVKNIQLEKKLNSLTDTVEKTQTQLVSVLSAANVDQTAHRGIINKVEVQLCVFLFGSSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATA3</accession>
    <name>A0A673ATA3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N(alpha)-acetyltransferase 80, NatH catalytic subunit</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ATA3"/>
    <dbReference type="Ensembl" id="ENSSORT00005032422.1">
        <property type="protein sequence ID" value="ENSSORP00005031542.1"/>
        <property type="gene ID" value="ENSSORG00005015011.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATA3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905502">
        <property type="term" value="F:acetyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039840">
        <property type="entry name" value="NAA80"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13538">
        <property type="entry name" value="N-ACETYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13538:SF4">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025441417" evidence="2">
        <location>
            <begin position="19"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="65"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="332" mass="36665" checksum="2C87248376EF5197" modified="2020-06-17" version="1">MELKLLFFGVSLFSEISLIPVHVSSTASTVHLTFTLGWSACDLTANAKLTELKMDEEVEEDPTSERLRAVPVHRRPDLLVPCADLVNAEWKRSQAARVQSLQKSCPEFPVCLVLLQGRGDAERLIGHARLSRVVGHGASLFVESVVVSKAERGRGFGRTLMEEAERYARRRGFKRLCLTTHDKQHFYAHLGYTLSTPVQNAGAMAAFVPMEMLLRFSRMSNEETQRSPQGRSHSGSTSLTPPPPPLSSLPPPPPPLPPSLLPLPLLLLFPPSLPLPLPLLLLLPPSLPLLPLLPLLPLLLLDLCFRPWTRLRTETLKASPSSGCTKTSEQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B0M8</accession>
    <name>A0A673B0M8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">N-alpha-acetyltransferase 20</fullName>
            <ecNumber evidence="9">2.3.1.254</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionine N-acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">N-acetyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">N-terminal acetyltransferase B complex catalytic subunit NAA20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">N-terminal acetyltransferase B complex catalytic subunit NAT5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NatB catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">naa20</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N-terminal L-methionyl-L-asparaginyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-asparaginyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50484"/>
            <dbReference type="Rhea" id="RHEA-COMP:12694"/>
            <dbReference type="Rhea" id="RHEA-COMP:12695"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133356"/>
            <dbReference type="ChEBI" id="CHEBI:133358"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N-terminal L-methionyl-L-aspartyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-aspartyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50480"/>
            <dbReference type="Rhea" id="RHEA-COMP:12692"/>
            <dbReference type="Rhea" id="RHEA-COMP:12693"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133045"/>
            <dbReference type="ChEBI" id="CHEBI:133063"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N-terminal L-methionyl-L-glutaminyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50492"/>
            <dbReference type="Rhea" id="RHEA-COMP:12698"/>
            <dbReference type="Rhea" id="RHEA-COMP:12699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133361"/>
            <dbReference type="ChEBI" id="CHEBI:133362"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>N-terminal L-methionyl-L-glutamyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutamyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50488"/>
            <dbReference type="Rhea" id="RHEA-COMP:12696"/>
            <dbReference type="Rhea" id="RHEA-COMP:12697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133359"/>
            <dbReference type="ChEBI" id="CHEBI:133360"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the N-terminal acetyltransferase B (NatB) complex which is composed of NAA20 and NAA25.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.254" evidence="9"/>
    <dbReference type="RefSeq" id="XP_029984388.1">
        <property type="nucleotide sequence ID" value="XM_030128528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B0M8"/>
    <dbReference type="FunCoup" id="A0A673B0M8">
        <property type="interactions" value="1560"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005036686.1">
        <property type="protein sequence ID" value="ENSSORP00005035731.1"/>
        <property type="gene ID" value="ENSSORG00005016876.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415085"/>
    <dbReference type="CTD" id="51126"/>
    <dbReference type="InParanoid" id="A0A673B0M8"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000015">
        <property type="entry name" value="N-alpha-acetyltransferase 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="21">
        <location>
            <begin position="10"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039120"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039529"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041220"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00042295"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042702"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00042743"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046112"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="186" mass="21286" checksum="00D296DDE024E7A8" modified="2020-06-17" version="1">MSTELLAKMTTLRPFTCDDLFKFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAEAPGGELMGYIMGKAEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMEMLEEISERKGGFFVDLFVRVSNQVAVNMYKRLGYSVYRTVIEYYSASNGEPDEDAYDMRKALSRDTEKKSIIPLPHPVRPEDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B176</accession>
    <name>A0A673B176_9TELE</name>
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        <recommendedName>
            <fullName evidence="14">Transmembrane protein 214</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tmem214</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Critical mediator, in cooperation with CASP4, of endoplasmic reticulum-stress induced apoptosis. Required or the activation of CASP4 following endoplasmic reticulum stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Constitutively interacts with CASP4; required for the localization of procaspase 4 to the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM214 family.</text>
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    <dbReference type="RefSeq" id="XP_029984632.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673B176"/>
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    </dbReference>
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        <property type="protein sequence ID" value="ENSSORP00005035129.1"/>
        <property type="gene ID" value="ENSSORG00005016369.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115415260"/>
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    <dbReference type="OrthoDB" id="10022292at2759"/>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR019308">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13448">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13448:SF0">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
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        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004477"/>
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            <dbReference type="ARBA" id="ARBA00007984"/>
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            <dbReference type="ARBA" id="ARBA00011720"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022703"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="77091" checksum="1FD3F3FCE1460C76" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B2A2</accession>
    <name>A0A673B2A2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSSORT00005036901.1">
        <property type="protein sequence ID" value="ENSSORP00005035945.1"/>
        <property type="gene ID" value="ENSSORG00005016956.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B2A2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF13">
        <property type="entry name" value="COLLAGEN ALPHA-3(VI) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025578871" description="VWFA domain-containing protein" evidence="8">
        <location>
            <begin position="23"/>
            <end position="1380"/>
        </location>
    </feature>
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        <location>
            <begin position="23"/>
            <end position="195"/>
        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="956"/>
        </location>
    </feature>
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        <location>
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            <end position="1146"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005035945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1380" mass="151356" checksum="51C24323172853AC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B4W4</accession>
    <name>A0A673B4W4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 14C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tmem14ca</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for normal heme biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B4W4"/>
    <dbReference type="FunCoup" id="A0A673B4W4">
        <property type="interactions" value="712"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005037345.1">
        <property type="protein sequence ID" value="ENSSORP00005036384.1"/>
        <property type="gene ID" value="ENSSORG00005017146.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5620at2759"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:heme biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of heme biosynthetic process"/>
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        <property type="entry name" value="Transmembrane protein 14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005036384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="140" mass="14373" checksum="DA04493D13D4C412" modified="2020-06-17" version="1">MFPYLLVAQLVVCNSSVLHHSFSPVGFFAGVSNMSVDWAGYGYAALVASGGVAGYVKAGSVPSLAAGLLFGGLAGFGAYQISNDPNNIWVSLATSGTLSAVMGKRFYGSRKFMPAGLMAGASLLMVGKLSLALLQKPNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B526</accession>
    <name>A0A673B526_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">G protein-coupled receptor 37a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">gpr37a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673B526"/>
    <dbReference type="FunCoup" id="A0A673B526">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038350.1">
        <property type="protein sequence ID" value="ENSSORP00005037376.1"/>
        <property type="gene ID" value="ENSSORG00005017550.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B526"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:receptor complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:prosaposin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15127">
        <property type="entry name" value="7tmA_GPR37"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G protein-coupled receptor 37"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003909">
        <property type="entry name" value="GPR37_orph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46216:SF3">
        <property type="entry name" value="PROSAPOSIN RECEPTOR GPR37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46216">
        <property type="entry name" value="PROSAPOSIN RECEPTOR GPR37 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01421">
        <property type="entry name" value="GPR37ORPHANR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="304"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="90"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="430" mass="47622" checksum="39692F6EC41EA961" modified="2020-06-17" version="1">LRGRGGEDGAQPVVMAQEQDAEAPFGLNTSDYEEEYILPDFPDSTPFSPVDTRIRRKQVKNPFYPVTAESYGAYAVMITAAVIFSVGIIGNVSIMCIVCHNYYMRSISNSLLANLALWDFVIIFFCLPLVVFHALTKDWLLGELSCRIIPYLEVASLGVTTFTLCALCIDRFRAATNVQMYYEMIENWASTTAKLAVIWVGALLLALPELLIRQLVTEDGDPPDVTPCERCVVRISTELPDTLYVLGLTYDGARLWWYFGCYFCLPTLFTICSSLATARKIRRAERACVRGSKKQIQLESQMNCAVVALAILYGFCIIPENICNILSVYMAAGIPRRTLDILHLVSQLLLFCKSAVTPVLLFCLCQPFTRAFLDCCCCCCDECGPARSPATASDTENECTTTELELSPFSTIRMEAASTSAAYTAVGTHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673B5S8</accession>
    <name>A0A673B5S8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Beta-enolase</fullName>
            <ecNumber evidence="5">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">eno3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:10165"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="16">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A673B5S8"/>
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        <property type="interactions" value="871"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005038633.1">
        <property type="protein sequence ID" value="ENSSORP00005037651.1"/>
        <property type="gene ID" value="ENSSORG00005017484.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673B5S8"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BETA-ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENOLASE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-0456">Lyase</keyword>
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    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="32"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="18">
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            <begin position="171"/>
            <end position="457"/>
        </location>
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        <location>
            <begin position="438"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="439"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="372"/>
        </location>
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            <position position="187"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
            <position position="196"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <location>
            <position position="322"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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            <position position="322"/>
        </location>
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            <name>substrate</name>
        </ligand>
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        <location>
            <position position="347"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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            <position position="347"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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            <begin position="399"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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            <position position="423"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00011473"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        </source>
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            <dbReference type="SMART" id="SM01193"/>
        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="467" mass="50824" checksum="04DCCAFCAA36B618" modified="2020-06-17" version="1">HTHTHSVGKWSGPCPSLVLTTCPLCPPVRMSITKIHAREILDSRGNPTVEVDLWTAKGMFRAAVPSGASTGVHEALELRDGDKARYLGKGTIKAVDHVNKDIAPKLIEKNFSVIEQEKIDKFMLELDGTENKSQFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGHKDVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFHEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIEKAGYPDKIIIGMDVAASEFFRSGKYDLDFKSPDDPSRHISGEQLGDLYRNFIKNYPVQSIEDPFDQDDWENWTKFTGSVDIQVVGDDLTVTNPKRIQQAVDKKACNCLLLKVNQIGSVTESIQACKLAQSSGWGVMVSHRSGETEDTFISDLVVGLCTGQIKTGAPCRSERLAKYNQLMRNSETRPSSPVKTSATRRSTKATPLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BB31</accession>
    <name>A0A673BB31_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">Protein CASP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cux1b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CASP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BB31"/>
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        <property type="protein sequence ID" value="ENSSORP00005037832.1"/>
        <property type="gene ID" value="ENSSORG00005017372.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BB31"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012955">
        <property type="entry name" value="CASP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057476">
        <property type="entry name" value="Cux_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF15">
        <property type="entry name" value="PROTEIN CASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08172">
        <property type="entry name" value="CASP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25398">
        <property type="entry name" value="CUX1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="613"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Cux N-terminal" evidence="14">
        <location>
            <begin position="4"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="CASP C-terminal" evidence="13">
        <location>
            <begin position="415"/>
            <end position="634"/>
        </location>
    </feature>
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        <location>
            <begin position="255"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="496"/>
            <end position="544"/>
        </location>
    </feature>
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        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005037832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="665" mass="76298" checksum="2978291449FCB28F" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BD87</accession>
    <name>A0A673BD87_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ndufa12</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the complex I NDUFA12 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030006611.1">
        <property type="nucleotide sequence ID" value="XM_030150751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BD87"/>
    <dbReference type="FunCoup" id="A0A673BD87">
        <property type="interactions" value="1478"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039575.1">
        <property type="protein sequence ID" value="ENSSORP00005038582.1"/>
        <property type="gene ID" value="ENSSORG00005018025.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430631"/>
    <dbReference type="CTD" id="55967"/>
    <dbReference type="InParanoid" id="A0A673BD87"/>
    <dbReference type="OrthoDB" id="274641at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT B17.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="147" mass="17080" checksum="AB98833DE20B2954" modified="2020-06-17" version="1">MAEYANLVRRALGQLRGHAGVKGFLLQLFRVNDVKVGSLIGVDKYGNKYYEDKKHYFFGRHRWVIYTTEMNGKNTLWEVDGSMVPAEWHRWLHCMTEDPPTTHPPEPKKFLAEVHQFNVSGSPQQYVPYPTTRKKIHEWVPPKAGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BEC2</accession>
    <name>A0A673BEC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mettl18</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="RefSeq" id="XP_030011062.1">
        <property type="nucleotide sequence ID" value="XM_030155202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BEC2"/>
    <dbReference type="FunCoup" id="A0A673BEC2">
        <property type="interactions" value="1497"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005039959.1">
        <property type="protein sequence ID" value="ENSSORP00005038957.1"/>
        <property type="gene ID" value="ENSSORG00005018221.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433738"/>
    <dbReference type="CTD" id="92342"/>
    <dbReference type="InParanoid" id="A0A673BEC2"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="188"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005038957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="354" mass="39674" checksum="2E515D88405F7DB3" modified="2020-06-17" version="1">MSFCFNFDVQVQATNPDDVDRDDLQKVKDTAVKPTEGIPEPAEPVKEAKEHHPPSDPHFLLVDAVPETVNIGTLPPLHFLNETVFEKTSTEREDEEKILSRTVEQRSDLISGVYEGGLKVWECTYDLLELIEKDVETFGGKTVLDLGCGAGLLGILALKRGAGEVHFQDYNSTVIEQVTVPNVMLNCQDDDVDSEEDNEGKAKGKKQKEDGCKKKLTEKQEERDGKPPPKKRALDISQHPLLAKCRFFSGDWSTFLDLVIKEDPQPKYDLIFTSETIYNTSYYPSLHDTLHRLLAPDGLVYLATKSHYFGVGGGLHLFETFVGEKGIFSIDHLWDGEEGLQRHVVGLRFKGKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BER8</accession>
    <name>A0A673BER8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcyclin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cacybp</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030017058.1">
        <property type="nucleotide sequence ID" value="XM_030161198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BER8"/>
    <dbReference type="Ensembl" id="ENSSORT00005040105.1">
        <property type="protein sequence ID" value="ENSSORP00005039102.1"/>
        <property type="gene ID" value="ENSSORG00005018275.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437845"/>
    <dbReference type="CTD" id="27101"/>
    <dbReference type="InParanoid" id="A0A673BER8"/>
    <dbReference type="OrthoDB" id="164025at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044548">
        <property type="term" value="F:S100 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06468">
        <property type="entry name" value="p23_CacyBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000006">
        <property type="entry name" value="calcyclin-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.10:FF:000006">
        <property type="entry name" value="calcyclin-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037201">
        <property type="entry name" value="CacyBP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052289">
        <property type="entry name" value="Calcyclin-binding_UBL-bridge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037893">
        <property type="entry name" value="CS_CacyBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015120">
        <property type="entry name" value="Siah-Interact_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13164:SF3">
        <property type="entry name" value="CALCYCLIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13164">
        <property type="entry name" value="CALICYLIN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09032">
        <property type="entry name" value="Siah-Interact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140106">
        <property type="entry name" value="Calcyclin-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CS" evidence="12">
        <location>
            <begin position="71"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="SGS" evidence="11">
        <location>
            <begin position="149"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="227" mass="26396" checksum="8B1A1D5268616E27" modified="2020-06-17" version="1">MDLTEQINQLEADLSELGSLLEKAERKRVQELLKQEQKKVEKELSVKKQQKEQQARREADPTAATKSAYTVKITNYAWDQSDKFVKIYITLKDVHKIPSENVEITFSERSFSVLVKSLDGKNHQLTILNLLYPIDEKDSYKKIKTDMVLVMCKKQTTEKWECLTKVEKQSKEKDKPNTDDSSDPSEGLMNMLKKIYSEGDDEMKRTINKAWTESQEKKMKGGDMMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFB1</accession>
    <name>A0A673BFB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BFB1"/>
    <dbReference type="Ensembl" id="ENSSORT00005041005.1">
        <property type="protein sequence ID" value="ENSSORP00005039969.1"/>
        <property type="gene ID" value="ENSSORG00005018653.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BFB1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="197"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26983" checksum="AFCB3C7D3132B35C" modified="2020-06-17" version="1">MEQKGVQLDQETISCSICLDLLKDPVTIPCGHSYCMSCIQTHWDEENGKNIHSCLQCRQTFTPRPVLVKNTMLAVLVEQLKKTGLEPAAADHCYAGAEDVVCDVCTGRKLKAVKSCLVCLVSYCHKHLQPHYESAAFKKHKLVDPSEKLQENVCSLHDEVMKMFCRTDRQCICYLCSVDEHKGHDTVSAAAERTERQKELEVSRRKIQQRIKDKQKDVKVLQKEVEDINTALFPKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BFQ7</accession>
    <name>A0A673BFQ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox protein Hox-A11a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hoxa11a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BFQ7"/>
    <dbReference type="FunCoup" id="A0A673BFQ7">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040185.1">
        <property type="protein sequence ID" value="ENSSORP00005039178.1"/>
        <property type="gene ID" value="ENSSORG00005018315.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BFQ7"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000166">
        <property type="entry name" value="homeobox protein Hox-C11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021918">
        <property type="entry name" value="DUF3528"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12045">
        <property type="entry name" value="DUF3528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="224"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="226"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="163"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="298" mass="33472" checksum="DB3012EF8537622C" modified="2020-06-17" version="1">MMDFDERVPVGSNMYLPSCTYYVSAGADFSGLPSFLSQTPSTRPMTYSYSSNLPQVQPVREVTFRDYAAIDASGKWPHRGNLPQCYPSAEDMVHRDCLSAQTAVVGDMFGKNSPAAAAAAYHHHHHHHTSAGSAASNFYGNVGRNGVLPQAFDQFYETAYGNASESSSANGYSGDKTANKQPSSAPEDAASCRDTEAKDPRDETSSPELSSGNNEEKSSSSSGQRTRKKRCPYSKYQIRELEREFFFSVYINKEKRLQLSRMLNLTDRQVKIWFQNRRMKEKKLNRDRLQYYTSNPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BG63</accession>
    <name>A0A673BG63_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Early growth response protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">egr1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Probable transcription factor involved in muscle spindle development.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030001593.1">
        <property type="nucleotide sequence ID" value="XM_030145733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BG63"/>
    <dbReference type="FunCoup" id="A0A673BG63">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005040623.1">
        <property type="protein sequence ID" value="ENSSORP00005039612.1"/>
        <property type="gene ID" value="ENSSORG00005018469.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115427239"/>
    <dbReference type="CTD" id="1958"/>
    <dbReference type="InParanoid" id="A0A673BG63"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048880">
        <property type="term" value="P:sensory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000392">
        <property type="entry name" value="early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000324">
        <property type="entry name" value="Early growth response protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000419">
        <property type="entry name" value="Early growth response protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021839">
        <property type="entry name" value="EGR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021849">
        <property type="entry name" value="EGR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF42">
        <property type="entry name" value="EARLY GROWTH RESPONSE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11914">
        <property type="entry name" value="DUF3432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11928">
        <property type="entry name" value="DUF3446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 17" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="131"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="133"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005039612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="511" mass="55083" checksum="BF27A7CE83380FFD" modified="2020-06-17" version="1">MAAAKTEMILPALQISEPLSFPHSPMDNYPKLEEVMMISSGTPFLTASAPEGAGFGSGEPGEQYDHLAGDTLPDIPFNCEKPVSEQTYPTQRLPPISYTGRFTLEPATSCSNSLWAEPILGLFTGLMSNVPPSSSSSSVASQTTSSSSSTAPSSSSSSASTSSSSQSSSLSSSIHHSEPNPIYSAAPTYSSPNSDIFPDQGQAFQTSTGAVQYPPPAYPNGKTCSTSFPVPMIPDYLFPQQQGEISLVPPDQKPFQSQSSQPSLTPLSTIKAFATQTGSQDLKSVYQSQLIKPSRMRKYPTRPSKTPPHERPYACPVETCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACEICGRKFARSDERKRHTKIHLRQKDKKAEKAGAVVVTAAPVSAASPASSYPSPITSYPSPVSSYPSPVTSCYSSPVHTSYPSPSVATTYPSVSMSNTFQSQVASSFPSSVASNIYSSPIPTPLSDMQTTLSPRTIEIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIK3</accession>
    <name>A0A673BIK3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C-type lectin domain family 12 member B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC115413285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_029981888.1">
        <property type="nucleotide sequence ID" value="XM_030126028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BIK3"/>
    <dbReference type="Ensembl" id="ENSSORT00005041524.1">
        <property type="protein sequence ID" value="ENSSORP00005040482.1"/>
        <property type="gene ID" value="ENSSORG00005018890.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413285"/>
    <dbReference type="InParanoid" id="A0A673BIK3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051379">
        <property type="entry name" value="C-type_Lectin_Receptor_IMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746:SF9">
        <property type="entry name" value="CD209 ANTIGEN-LIKE PROTEIN C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="147"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="276" mass="31649" checksum="FF6A532EF73B1541" modified="2020-06-17" version="1">MGITQRWTNSNRDSTINESNYRLLLLCLGLLNGALLIVAVAIGIKCATVKQGSDQVSHTAASELISKLKYLRSSHSDMIEAEEEAKKQLEKVLSDHANLKMQLEHQKEINDGCQKGIEDLHTEQALLQSNLSALDTCGKCLPGWMIFNSSCYLFNNNLGQGLTWKESRDDCIQRGGDLVVIDYPEEEIFLRNTINWFEMYNRNRFWIGISFVERAKTFVWLNNVTQAIRPVDRSIMFTYSHTCGYIWNSLSTRDCYERSNWICEMQTNQSVDVLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BIU0</accession>
    <name>A0A673BIU0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673BIU0"/>
    <dbReference type="Ensembl" id="ENSSORT00005041295.1">
        <property type="protein sequence ID" value="ENSSORP00005040258.1"/>
        <property type="gene ID" value="ENSSORG00005018780.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BIU0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002494">
        <property type="entry name" value="KAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21523">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21523:SF14">
        <property type="entry name" value="EXPORTED REPETITIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13885">
        <property type="entry name" value="Keratin_B2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005040258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="268" mass="27347" checksum="DCEC440175213791" modified="2020-06-17" version="1">METEGLPGLLWSDPHAVCDPAVSDPSVCDPSAVCDPSVCDPSVCDPAVCDPAVCDPSVCDPSVCDPAVCDPSVCDPSAVCDHSVCDPSVCDPAVCDPSVCDPSVCDPSAVCDPSVCDPSAVFCDPSVCDPSVCDPAVCDPSVCDPSTVCDPSSVCDPSSVCDPSAVCDHSVCDPSVCDPAVCDPSVCDPSTVCDPSSVCDPSVCDPSAVCDHSVCDPSVCDPAVCDPSTTCTRLRPGTVLWSKGTKQEVLIMLTVPGKRGWRRCMSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BKE8</accession>
    <name>A0A673BKE8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat and SOCS box protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">asb12b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with CUL5 and RNF7.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BKE8"/>
    <dbReference type="FunCoup" id="A0A673BKE8">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005043320.1">
        <property type="protein sequence ID" value="ENSSORP00005042245.1"/>
        <property type="gene ID" value="ENSSORG00005019580.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BKE8"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000096">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="94"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="255"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005042245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="311" mass="34474" checksum="FC66747A0205D03B" modified="2020-06-17" version="1">MSLMDISKIFSLLQPKDEDEDNEQCQALNNAVSSDNVTLLSELLSQEAYKRSINGRSGWGVPVTPLRTAAALGHLRCLELLLEHGAEIDSLDVKAQTPLFTAVSGKHLDCVVALLKSGADPNGSQYNNCSPVLTAAREGDVDVLRELLRFGAEVDVRPKVPDWASNATACRGPLYISAVYGHLDCFKLLLLHGANPNYNCTDEKMLARIKQPKTVLEVCLRYGCGVEYIQLLIDFGADVYLPTLIIDKMTKQNEALVLLFKERVCPKTLMSQTRLAIRRFLPNADKNPAIDRLDIPPILRNYLKHNTSELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BLL9</accession>
    <name>A0A673BLL9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tspan17</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BLL9"/>
    <dbReference type="Ensembl" id="ENSSORT00005042224.1">
        <property type="protein sequence ID" value="ENSSORP00005041163.1"/>
        <property type="gene ID" value="ENSSORG00005019179.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BLL9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03159">
        <property type="entry name" value="TM4SF9_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000001">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF470">
        <property type="entry name" value="TETRASPANIN-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005041163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="339" mass="38295" checksum="62064640AEDC0A11" modified="2020-06-17" version="1">MSRKHHHFKGAEVSCCVKYFLFGFNIIFWLLGAAFLGIGLWAWAEKGVLSNLSSITDLGGFDPVWLFIVVGGVMFILGFAGCIGALRENTFLLKFFSVFLGLIFFLELTAGILAFVFKDWIKDQLNFFINNNVKAYRDDIDLQNLIDFAQEYWSCCGAHGPDDWNLNIYFNCTELNPSRERCGVPFSCCVKDPAEDVINTQCGYDVRLQGELDRQKYIYTKGCVGQFEKWLQDNLIIVAGIFVGIALLQIFGICLAQNLVSDVKAVKLWDGEPRTSISLYSANELSSTKTHIRLDTKSLLPCLSVKIPYNKPKPSTERLFTTLMGFYPNIQRTVLPSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BND5</accession>
    <name>A0A673BND5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Regulating synaptic membrane exocytosis 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rims1b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
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        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
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        <source>
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        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Sphaeramia orbicularis</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005043483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15775" checksum="4E4DEDD83DD0CBEF" modified="2020-06-17" version="1">MGCLGNSKTEDQRIDEKAQREANKKIEKQLQKERQAYKATHRLLLLGAGESGKSTIVKQMRILHVNGFNAEEKKQKIQDIRKNVKDAIVHFNTPVPLVKPEDQFRIEYIKSIAPLSDFDYSQVSCSQSFCLSTVCACGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BWG0</accession>
    <name>A0A673BWG0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fibrillar collagen NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673BWG0"/>
    <dbReference type="Ensembl" id="ENSSORT00005048425.1">
        <property type="protein sequence ID" value="ENSSORP00005047251.1"/>
        <property type="gene ID" value="ENSSORG00005021608.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673BWG0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000002">
        <property type="entry name" value="Collagen XI alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000885">
        <property type="entry name" value="Fib_collagen_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1082">
        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01410">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51461">
        <property type="entry name" value="NC1_FIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Fibrillar collagen NC1" evidence="6">
        <location>
            <begin position="290"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="249"/>
        </location>
    </feature>
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        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="519" mass="54483" checksum="B11437CCA1F961FE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BYL1</accession>
    <name>A0A673BYL1_9TELE</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized LOC115411133</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115411133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C65 family. Otulin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029978948.1">
        <property type="nucleotide sequence ID" value="XM_030123088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BYL1"/>
    <dbReference type="FunCoup" id="A0A673BYL1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005049254.1">
        <property type="protein sequence ID" value="ENSSORP00005048066.1"/>
        <property type="gene ID" value="ENSSORG00005021949.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411133"/>
    <dbReference type="InParanoid" id="A0A673BYL1"/>
    <dbReference type="OrthoDB" id="5962728at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990108">
        <property type="term" value="P:protein linear deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023235">
        <property type="entry name" value="FAM105"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023236">
        <property type="entry name" value="OTULINL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33662:SF3">
        <property type="entry name" value="FIBROUS SHEATH CABYR-BINDING PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33662">
        <property type="entry name" value="OTU DEUBIQUITINASE WITH LINEAR LINKAGE-SPECIFICITY A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16218">
        <property type="entry name" value="Peptidase_C101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02055">
        <property type="entry name" value="PROTEINF105"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02056">
        <property type="entry name" value="PROTEINF105A"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="68"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="172"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="101"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="239"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="320"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005048066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="740" mass="79892" checksum="3E422C5656B5E600" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673BZH9</accession>
    <name>A0A673BZH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Isovaleryl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="6">1.3.8.1</ecNumber>
            <ecNumber evidence="5">1.3.8.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Butyryl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC115413747</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway. To a lesser extent, is also able to catalyze the oxidation of other saturated short-chain acyl-CoA thioesters as pentanoyl-CoA, hexenoyl-CoA and butenoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = 3-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:12276"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57389"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21 22">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="6"/>
    <dbReference type="EC" id="1.3.8.4" evidence="5"/>
    <dbReference type="RefSeq" id="XP_029982666.1">
        <property type="nucleotide sequence ID" value="XM_030126806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673BZH9"/>
    <dbReference type="Ensembl" id="ENSSORT00005048333.1">
        <property type="protein sequence ID" value="ENSSORP00005047160.1"/>
        <property type="gene ID" value="ENSSORG00005021572.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413747"/>
    <dbReference type="InParanoid" id="A0A673BZH9"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="UniPathway" id="UPA00363">
        <property type="reaction ID" value="UER00860"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01156">
        <property type="entry name" value="IVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000007">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000003">
        <property type="entry name" value="isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000004">
        <property type="entry name" value="Isovaleryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034183">
        <property type="entry name" value="IVD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21 22" id="KW-0274">FAD</keyword>
    <keyword evidence="8 22" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="25">
        <location>
            <begin position="39"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="24">
        <location>
            <begin position="157"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="23">
        <location>
            <begin position="264"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="371"/>
            <end position="375"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="398"/>
            <end position="399"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="400"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR634183-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005047160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="417" mass="45920" checksum="D0CE087A116D7A14" modified="2020-06-17" version="1">MFAVRNALCVGSRLSICRVLRRGCAGASIPVDDVVNGLTEEQIQLRHSVRKFYAKHLAPHADEIDKTSDFPGMRGFWKELGEMGLLGITAPVEYGGTGLGFLDHIIVVEEMSRVSAAISLSYGAHSNLCVNQIVRHANEKQKEKYLPKLFTGEHVGAMAMSESNAGSDVTSMKLKATKKGDYYVLNGHKFWITNGPDADVLVVYAKTDPEAHQKGISTFIVEKGMPGFSSAQKLDKLGMRGSNTCELIFEDCKVPEENVLGILNKGVYVMMSGLQLERLVLAAGPIGVMQAVIDFAFPYLHVREAFGQKIGHFQLMQGKMADMYTRLSCCRQYLYNVARACDKGHFNPADCVGVLLYCAENATQVALDGIQCLGGNGYINDYPMGRFLRDAKLYEIAGGTNEIRRLIIGRTFNSMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C0D1</accession>
    <name>A0A673C0D1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps15</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C0D1"/>
    <dbReference type="FunCoup" id="A0A673C0D1">
        <property type="interactions" value="1519"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005047546.1">
        <property type="protein sequence ID" value="ENSSORP00005046387.1"/>
        <property type="gene ID" value="ENSSORG00005021234.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C0D1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
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        <property type="entry name" value="Ribosomal_uS19_CS"/>
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    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="149" mass="17492" checksum="6CBAE0B05D139008" modified="2020-06-17" version="1">KRTKLADTEIKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYCARQRRRLNRGLRRKQQSLLKRLRKAKKEAPPMEKPEVVKTHLRDMVILPEMVGSMVGVYNGKTFNQVEIKPEMCGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1G2</accession>
    <name>A0A673C1G2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Short transient receptor potential channel 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115432454</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030009163.1">
        <property type="nucleotide sequence ID" value="XM_030153303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673C1G2"/>
    <dbReference type="Ensembl" id="ENSSORT00005047372.1">
        <property type="protein sequence ID" value="ENSSORP00005046213.1"/>
        <property type="gene ID" value="ENSSORG00005021173.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432454"/>
    <dbReference type="InParanoid" id="A0A673C1G2"/>
    <dbReference type="OrthoDB" id="25840at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000222">
        <property type="entry name" value="Short transient receptor potential channel 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013555">
        <property type="entry name" value="TRP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005458">
        <property type="entry name" value="TRPC2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF6">
        <property type="entry name" value="SHORT TRANSIENT RECEPTOR POTENTIAL CHANNEL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08344">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01097">
        <property type="entry name" value="TRNSRECEPTRP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01643">
        <property type="entry name" value="TRPCHANNEL2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01420">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140860">
        <property type="entry name" value="Pseudo ankyrin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Transient receptor ion channel" evidence="12">
        <location>
            <begin position="174"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="815"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="817"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="912"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="928"/>
            <end position="990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005046213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1034" mass="117940" checksum="F847715EA1ABE6F7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C1M5</accession>
    <name>A0A673C1M5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Aminopeptidase</fullName>
            <ecNumber evidence="23">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">erap1b</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21 23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21 23">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. May also exist as a heterodimer; with ERAP2. Interacts with RBMX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 23">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="23"/>
    <dbReference type="AlphaFoldDB" id="A0A673C1M5"/>
    <dbReference type="Ensembl" id="ENSSORT00005048013.1">
        <property type="protein sequence ID" value="ENSSORP00005046842.1"/>
        <property type="gene ID" value="ENSSORG00005020769.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C1M5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000007">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000003">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF156">
        <property type="entry name" value="ENDOPLASMIC RETICULUM AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        </location>
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        <source>
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    <sequence length="949" mass="108770" checksum="015090C9AF3C8B36" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C5E4</accession>
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        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Required for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Dynein axonemal particle</location>
        </subcellularLocation>
        <text evidence="3">Localizes in the apical cytoplasm around the gamma-tubulin-positive pericentriolar region, not in the cilia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIH1 family. Kintoun subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673C5E4"/>
    <dbReference type="Ensembl" id="ENSSORT00005050393.1">
        <property type="protein sequence ID" value="ENSSORP00005049195.1"/>
        <property type="gene ID" value="ENSSORG00005022353.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C5E4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003351">
        <property type="term" value="P:epithelial cilium movement involved in extracellular fluid movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03069">
        <property type="entry name" value="Kintoun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034727">
        <property type="entry name" value="Kintoun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF3">
        <property type="entry name" value="PROTEIN KINTOUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="5">
        <location>
            <begin position="40"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="6">
        <location>
            <begin position="241"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="344"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="212"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="359"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="422"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005049195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="553" mass="62799" checksum="87A9EEA2845663DA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673C9W0</accession>
    <name>A0A673C9W0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Ras-related protein Rab-35</fullName>
            <ecNumber evidence="8">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">rab35b</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A673C9W0"/>
    <dbReference type="SMR" id="A0A673C9W0"/>
    <dbReference type="Ensembl" id="ENSSORT00005051273.1">
        <property type="protein sequence ID" value="ENSSORP00005050069.1"/>
        <property type="gene ID" value="ENSSORG00005022718.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673C9W0"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000404">
        <property type="entry name" value="Putative ras-related protein Rab-35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0168">Coated pit</keyword>
    <keyword evidence="23" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00067828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="201" mass="22975" checksum="5334000B404C7B48" modified="2020-06-17" version="1">MARDYDYLFKLLIIGDSGVGKSSLLLRFADNTFSGSYITTIGVDFKIRTVEINGEKVKLQIWDTAGQERFRTITSTYYRGTHGVIVVYDVTSAESFVNVKRWLHEINQNCDDVCRILVGNKNDDPNSKVVETTDAQKFAEQMGINLFETSAKENINVEEMFNCITELVLRAKKEVLAKQQQQQQNDVVKLTRNSKRKKKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CAC8</accession>
    <name>A0A673CAC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ZBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">fbxo5</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_030011099.1">
        <property type="nucleotide sequence ID" value="XM_030155239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CAC8"/>
    <dbReference type="FunCoup" id="A0A673CAC8">
        <property type="interactions" value="1148"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053769.1">
        <property type="protein sequence ID" value="ENSSORP00005052521.1"/>
        <property type="gene ID" value="ENSSORG00005023663.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433752"/>
    <dbReference type="CTD" id="26271"/>
    <dbReference type="InParanoid" id="A0A673CAC8"/>
    <dbReference type="OrthoDB" id="9984940at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045835">
        <property type="term" value="P:negative regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20348">
        <property type="entry name" value="BRcat_RBR_EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000006">
        <property type="entry name" value="F-box only protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047147">
        <property type="entry name" value="FBX5_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044064">
        <property type="entry name" value="ZF_ZBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15493:SF8">
        <property type="entry name" value="F-BOX ONLY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15493">
        <property type="entry name" value="F-BOX ONLY PROTEIN 5 AND 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51872">
        <property type="entry name" value="ZF_ZBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZBR-type" evidence="15">
        <location>
            <begin position="370"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="112"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="446" mass="49815" checksum="FCED357712D65423" modified="2020-06-17" version="1">MPHSKRIIMKFPRQEASRTFSMDKNSAAAESKVLHLKSSPVKEPPPCKPLVTPADVTLVLSHNNDTKAVHNKENNTNGKDIDRIVGERLEDSGYLSLHNSQIEEEDDHFHVQAPTTLPPSAATHQQKTISPKPSPTKCQGRMDSVSPVASTPVLRQKRVDTYSMSSTPSDSNNNPYLPILKFEQAVCEELAKSFQKTKRYDWSVITKVAEDHLLDRVIGRQMGLAYIDIFSSLLTRNMRGILTCILALLGDMDLISCQKVSKTWRKIICEDSAALKRCQQAEQALRESGSSLRQKTSGLTRDVAVSRMVLSCVQSLASPNVSSSSPLSSIQSCKNNRLLESSSKGRMPNTDCTRFKEYVEAASTLKQHESLRHCKRCNSPATHFPETQRATCTRLSCLFDFCTRCQEGFHGSMPCRVVQPRPHFNTSKTTTILPGSARSKRNIRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBD7</accession>
    <name>A0A673CBD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Copine-7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cpne7</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CBD7"/>
    <dbReference type="FunCoup" id="A0A673CBD7">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052610.1">
        <property type="protein sequence ID" value="ENSSORP00005051385.1"/>
        <property type="gene ID" value="ENSSORG00005023214.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CBD7"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000132">
        <property type="entry name" value="Copine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000163">
        <property type="entry name" value="Copine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF6">
        <property type="entry name" value="COPINE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="137"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="307"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="563" mass="63190" checksum="720B961B817A8469" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CBP1</accession>
    <name>A0A673CBP1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">GPI alpha-1,4-mannosyltransferase I, catalytic subunit</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pigm</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 13">Catalytic subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGM family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_029978803.1">
        <property type="nucleotide sequence ID" value="XM_030122943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CBP1"/>
    <dbReference type="FunCoup" id="A0A673CBP1">
        <property type="interactions" value="1255"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005051370.1">
        <property type="protein sequence ID" value="ENSSORP00005050162.1"/>
        <property type="gene ID" value="ENSSORG00005022759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115411025"/>
    <dbReference type="CTD" id="93183"/>
    <dbReference type="InParanoid" id="A0A673CBP1"/>
    <dbReference type="OrthoDB" id="1741594at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051751">
        <property type="term" value="F:alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005050162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="414" mass="47810" checksum="A55469FEAA738183" modified="2020-06-17" version="1">MERLITKLTEKHVLFTASFAIRLALVAYGDFQDRTMVVKYTDVDYHVFTDAARFMTEGQSPYNRSTYRYTPLLAWLLTPNIYLSMVFGKILFIVCDVLSGLLIYKILHLRGLSSETALRVSSLWLFNPLPMGVSSRGNAESILSALVLGTLLCMEARRLKLAAVLYGLSVHMKIYPVTYALPIALTLRTSKPRVEEDKSGWRWRGLVGFIRSFINRDLLLFASVAGGVFCTLTALFYYMYGWEFLHETYLYHLTRRDTRHNFSPYFYMLYLTADTRWSQWLGLAAFLPQLTLLLVISWAFHPDLTLCCFLHTAIFVSFNKVCTSQYFLWYLCLLPLVIPHLSLSLKQGLGLLTLWFVGQAIWLGPAYFLEFEGYNTFLLIWLAGLLFLIINTFILIQVVTHYKQTKTPQRLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CE46</accession>
    <name>A0A673CE46_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4604 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">kiaa1143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030013572.1">
        <property type="nucleotide sequence ID" value="XM_030157712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CE46"/>
    <dbReference type="FunCoup" id="A0A673CE46">
        <property type="interactions" value="599"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055123.1">
        <property type="protein sequence ID" value="ENSSORP00005053856.1"/>
        <property type="gene ID" value="ENSSORG00005024179.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115435359"/>
    <dbReference type="CTD" id="57456"/>
    <dbReference type="InParanoid" id="A0A673CE46"/>
    <dbReference type="OrthoDB" id="10043580at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027911">
        <property type="entry name" value="DUF4604"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040219">
        <property type="entry name" value="KIAA1143-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31195">
        <property type="entry name" value="GEO02494P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31195:SF2">
        <property type="entry name" value="GEO02494P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15377">
        <property type="entry name" value="DUF4604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4604" evidence="2">
        <location>
            <begin position="35"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005053856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="185" mass="20341" checksum="A6DB19722EED1E0E" modified="2020-06-17" version="1">MLMTYICCGGHRCSFSPLVILNILTKTMNKGKASGVSWVKPSEPSFLKKFKNDVGYKEGPTVDTKRQVMPTLDDDSGSDKEDELPQVVVLKSGDLTAEEAKKIKEGTRGGATDKDDKPADGKILFKKPVKRSSSDKFQGITASSSKKKKSDEDEKEEEKKEVKSGKKVKNNSLLSFGGDEEEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CER6</accession>
    <name>A0A673CER6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 256-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC115439087</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM256 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030018842.1">
        <property type="nucleotide sequence ID" value="XM_030162982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CER6"/>
    <dbReference type="Ensembl" id="ENSSORT00005053843.1">
        <property type="protein sequence ID" value="ENSSORP00005052595.1"/>
        <property type="gene ID" value="ENSSORG00005023697.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053844.1">
        <property type="protein sequence ID" value="ENSSORP00005052596.1"/>
        <property type="gene ID" value="ENSSORG00005023697.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115439087"/>
    <dbReference type="OrthoDB" id="269173at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006696">
        <property type="entry name" value="DUF423"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43461">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43461:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04241">
        <property type="entry name" value="DUF423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005052595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="114" mass="11714" checksum="B9288647D8BCD754" modified="2020-06-17" version="1">MSASAVVRRLAALSGASAVGLGAYGAHGFKNSDPDDYRMVLFETANKYHFYHSLALLGAAHSGKPAVAGALLVAGMGMFCGSLYHQALTGDPGLRKVAPMGGMAMIAGWLAMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CF31</accession>
    <name>A0A673CF31_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">C-type lectin domain family 4 member K-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115426838</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030000931.1">
        <property type="nucleotide sequence ID" value="XM_030145071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CF31"/>
    <dbReference type="FunCoup" id="A0A673CF31">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005053163.1">
        <property type="protein sequence ID" value="ENSSORP00005051924.1"/>
        <property type="gene ID" value="ENSSORG00005023440.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426838"/>
    <dbReference type="InParanoid" id="A0A673CF31"/>
    <dbReference type="OrthoDB" id="8950604at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="109"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="225" mass="26076" checksum="A1F348B32F2A954C" modified="2020-06-17" version="1">MVKFDDSVGYESQASVSGRRVWINRCSGGDAIGRNPTGMKFLRLFIVSFGLLCLLQATLNITLRLTLITKRNLTDSCAAVIKNLTDENEKLKRTQDIFDEYIKQGWFYFSNHLYYKSETKKSWQESRQDCLQRGADLTIISSREEQVFVRNFLSAWIGLTDNETEGIWKWVDGTPLNTSYWHDKEPNNANSNENCGEIGYHGKLNTWNDKPCHSENYWICEKMVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CFS7</accession>
    <name>A0A673CFS7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein NDRG1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ndrg1a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CFS7"/>
    <dbReference type="FunCoup" id="A0A673CFS7">
        <property type="interactions" value="1526"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005055705.1">
        <property type="protein sequence ID" value="ENSSORP00005054431.1"/>
        <property type="gene ID" value="ENSSORG00005024317.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CFS7"/>
    <dbReference type="OrthoDB" id="741027at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000006">
        <property type="entry name" value="NDRG family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="383" mass="42564" checksum="FD0B46C5F594273D" modified="2020-06-17" version="1">MDDIQVVESKPLLVDKDLQGLREAVQQLVMKEHDVDTPHGRVHCTMKGVPKGDRPVILTFHDIGLNHKTCWDTLFNHEDMAEIMHHFAVCHVDAPGQHEGANTFSTGYEYPSMDQLAETLPLVLKHFGLKSVIGMGIGAGANILTRFALDYSNMVEGLVLININPCAEGWMDWAAHKLSGLTHDMTGMIITHLFGKEEIHHNHDLIATYRHHIINDMNQFNLHLFVKSYESRRDLEVERPVPGQNNRTLKCPSLLVVGDSSPAIEAVVECNTKLDPTKTTLLKMADCGGMPQVDQPGKLTEAFKYFIQGMGYMPAASMTRLVRSRTASGSSVTSFDGNRSRSHTHEGNRSRSHTSENRGRSHTDTMDSSNADQSVPKSTEVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CHG4</accession>
    <name>A0A673CHG4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rhbdd3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029998745.1">
        <property type="nucleotide sequence ID" value="XM_030142885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673CHG4"/>
    <dbReference type="FunCoup" id="A0A673CHG4">
        <property type="interactions" value="956"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005052729.1">
        <property type="protein sequence ID" value="ENSSORP00005051498.1"/>
        <property type="gene ID" value="ENSSORG00005023264.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425369"/>
    <dbReference type="CTD" id="25807"/>
    <dbReference type="InParanoid" id="A0A673CHG4"/>
    <dbReference type="OrthoDB" id="9908508at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF16">
        <property type="entry name" value="RHOMBOID DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="6">
        <location>
            <begin position="55"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005051498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="364" mass="40282" checksum="CE1E4FFDD0D00E85" modified="2020-06-17" version="1">MLDRILSVWFWFGSDRPGFFIGTSLLASFMLLIYAGGVQANLSVGPGGEFPRFRDVFLYAFSHDDSPSLLISVAFLLLVGPCQERRWGTVAFLSLSILTTALLPFIYTLVLFVGGVGASRFCGYSAIQLALFTAQCRQVTQRRLLRCLPVWFLPWLLLLLGLMLLPGTPALLHFCAICIGHNYRQSFIGTLQELEETRILDIIPDWTYVVTSARLRLPTYTTSRRSSSFSPVPKINEADHSLRDPYLLNQHPWVEPMPAWIMEQSATLSEAEVLEEQMLRAGILASLQDAPDNPDAKVEVPKSSVSSLRLQQLEKMGFPTEKAVVALAASKQLDGAISLLIDDSIGEEAVMVSTGKTSLPPKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CI07</accession>
    <name>A0A673CI07_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho GTPase-activating protein 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rho-type GTPase-activating protein 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">arhgap29a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673CI07"/>
    <dbReference type="Ensembl" id="ENSSORT00005052936.1">
        <property type="protein sequence ID" value="ENSSORP00005051703.1"/>
        <property type="gene ID" value="ENSSORG00005023274.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CI07"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
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            <begin position="236"/>
            <end position="498"/>
        </location>
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        <location>
            <begin position="674"/>
            <end position="719"/>
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            <end position="937"/>
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            <begin position="1"/>
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        </location>
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            <end position="1089"/>
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    </feature>
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        <location>
            <begin position="1208"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="27"/>
            <end position="42"/>
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            <begin position="54"/>
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            <end position="395"/>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="1254" mass="138555" checksum="3096ECD3412B9CC2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CIN5</accession>
    <name>A0A673CIN5_9TELE</name>
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            <fullName evidence="18">Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 1</fullName>
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            <fullName evidence="19">Leucine-rich repeat-containing protein 21</fullName>
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            <fullName evidence="21">Photoreceptor-associated LRR superfamily protein</fullName>
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        <alternativeName>
            <fullName evidence="20">Retina-specific protein PAL</fullName>
        </alternativeName>
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        <name evidence="27" type="primary">lrit1a</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="17">Photoreceptor synaptic protein essential for normal vision. Involved in synapse formation in cone photoreceptor cells.</text>
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            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
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    </dbReference>
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        <property type="entry name" value="LEURICHRPT"/>
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        <property type="entry name" value="IG"/>
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        <property type="entry name" value="LRRCT"/>
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        <property type="entry name" value="LRRNT"/>
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        <property type="entry name" value="Fibronectin type III"/>
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        <property type="entry name" value="Immunoglobulin"/>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6 24" id="KW-0732">Signal</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0844">Vision</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025424223" description="Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 1" evidence="24">
        <location>
            <begin position="23"/>
            <end position="652"/>
        </location>
    </feature>
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        <location>
            <begin position="553"/>
            <end position="578"/>
        </location>
    </feature>
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        <location>
            <begin position="252"/>
            <end position="344"/>
        </location>
    </feature>
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        <location>
            <begin position="456"/>
            <end position="544"/>
        </location>
    </feature>
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        <location>
            <begin position="349"/>
            <end position="370"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005055456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="652" mass="70958" checksum="41AE4616B75479B3" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSE5</accession>
    <name>A0A673CSE5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BCCIP homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC115410526</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the BCP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CSE5"/>
    <dbReference type="FunCoup" id="A0A673CSE5">
        <property type="interactions" value="1380"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005057580.1">
        <property type="protein sequence ID" value="ENSSORP00005056284.1"/>
        <property type="gene ID" value="ENSSORG00005025052.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSE5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028983">
        <property type="entry name" value="BCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005056284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="288" mass="32076" checksum="82241605ADBBE080" modified="2020-06-17" version="1">MFAVGMASSAKRRAVGLGENPEESDNSSDEDLEDDDDSGEEDSEASEEEINEEVVVDFEAHALTGSDFNGIKKLLQQLFLKAHVNTSEMTDIIIQQNHIGSVIKYREDSDDDDPDEVFGFITMLNLTERKGINTCKVKNCGHSVTEQLELILNDTSKPVGLLLSERFINVPPQIALPLHTQLQGEIAEAQRTNKPSGKCHYCLLISKTCKEASKTIPARGGGAPKEEYMFINQAILKFHYSVQEEADSCLSGRWSFDDVPMKPFRTVMLVPTDKMPAIMDKLKEYLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673CSR3</accession>
    <name>A0A673CSR3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Grass carp reovirus (GCRV)-induced gene 2p</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673CSR3"/>
    <dbReference type="FunCoup" id="A0A673CSR3">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005056234.1">
        <property type="protein sequence ID" value="ENSSORP00005054958.1"/>
        <property type="gene ID" value="ENSSORG00005024582.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673CSR3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.175.10">
        <property type="entry name" value="Diphtheria Toxin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36542">
        <property type="entry name" value="GIG2-LIKE PROTEIN DRED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36542:SF6">
        <property type="entry name" value="GIG2-LIKE PROTEIN DREP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARP catalytic" evidence="2">
        <location>
            <begin position="31"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005054958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="224" mass="24990" checksum="E68767DA0AC93FCC" modified="2020-06-17" version="1">MSVQFSGWEVIDDGASFPGVLLNSAQKPQNRGVYTMYHGTSINCAKLIISSGFKQSSNGMLGKGVYVSRDKRKASNYPLHSKTSDRVVLELRVRVGRVKRIDKDNHPLQYSWNAKGYDTAWVPPNCGMLSVPSGLEEDCVYDPKRVKVVGIAKAPDTATQKDLQQLLDKKSKRGQDGAANLCPQCKRKTQMGTPHIKQQCWACGQNICILMSKHFLYYNYSVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y623</accession>
    <name>A0A672Y623_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rap2b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_030008372.1">
        <property type="nucleotide sequence ID" value="XM_030152512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y623"/>
    <dbReference type="FunCoup" id="A0A672Y623">
        <property type="interactions" value="387"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005001686.1">
        <property type="protein sequence ID" value="ENSSORP00005001638.1"/>
        <property type="gene ID" value="ENSSORG00005001026.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115431841"/>
    <dbReference type="InParanoid" id="A0A672Y623"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032486">
        <property type="term" value="P:Rap protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04176">
        <property type="entry name" value="Rap2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001686">
        <property type="entry name" value="KRAS proto-oncogene, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002735">
        <property type="entry name" value="ras-related protein Rap-2b isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041840">
        <property type="entry name" value="Rap2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005001638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="183" mass="20505" checksum="ED6C51375D9575C6" modified="2020-06-17" version="1">MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVSSGEGKALADDWNCPFMETSAKNKSSVDELFAEIVRQMNYASTPNGDDQCCSSCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y839</accession>
    <name>A0A672Y839_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetylgalactosaminide beta-1,3-galactosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.122</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an N-acetyl-alpha-D-galactosaminyl derivative + UDP-alpha-D-galactose = a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15621"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28257"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="EC" id="2.4.1.122"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.122" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A672Y839"/>
    <dbReference type="Ensembl" id="ENSSORT00005002876.1">
        <property type="protein sequence ID" value="ENSSORP00005002793.1"/>
        <property type="gene ID" value="ENSSORG00005001734.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Y839"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016263">
        <property type="term" value="F:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026050">
        <property type="entry name" value="C1GALT1/C1GALT1_chp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033">
        <property type="entry name" value="BETA1,3-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="14">
        <location>
            <begin position="80"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="245" mass="27999" checksum="56186F5175CF7931" modified="2020-06-17" version="1">MRLLLSVSWTEEACYLGGRGGQEGAVGREGLPKGARKPSSILTGNDAESEEDFEPRIVPYKQVNRVQAKYISTELGMRERLFVGVLTSKNTISTLGVAVNRTISHHLDTVVFFTGMRNRKVPHGMFVVSHGDERLIWNMFQTIRYILDHYINEYDWFYLVQDDAYTEADRIRALVDHLSMDRELYMGSPEEFIGGEMEGKYCYGGFGYLLSRTLLLRLHARPDEWLGRCIIDYTSANCVSEYEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Y8W6</accession>
    <name>A0A672Y8W6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Replication factor A protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rpa2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030015116.1">
        <property type="nucleotide sequence ID" value="XM_030159256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Y8W6"/>
    <dbReference type="FunCoup" id="A0A672Y8W6">
        <property type="interactions" value="1349"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005003331.1">
        <property type="protein sequence ID" value="ENSSORP00005003233.1"/>
        <property type="gene ID" value="ENSSORG00005001975.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436395"/>
    <dbReference type="CTD" id="6118"/>
    <dbReference type="InParanoid" id="A0A672Y8W6"/>
    <dbReference type="OrthoDB" id="25571at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04478">
        <property type="entry name" value="RPA2_DBD_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000168">
        <property type="entry name" value="Replication protein A 32 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000149">
        <property type="entry name" value="Replication protein A 32 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014646">
        <property type="entry name" value="Rfa2/RPA32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036949">
        <property type="entry name" value="RPA32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A C-terminal" evidence="13">
        <location>
            <begin position="200"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="32"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="11">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="11">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036949-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005003233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="318" mass="34348" checksum="8ADBE24985132AA7" modified="2020-06-17" version="1">MDVKKKPGETEKARNKYEAKYKYKDFKRKSFTRGHKKTRLTQGGYSESMGGGYTQSPGGFASPALSQGGEKKGRTRATQIIPCTVSQLMSASQADEAFRVGDVEVAQVTIVGVIRSTDKSMTNIQYKVDDMTGAPMDVKQWVDTEDPGVDSTVLPPGTYVKVSGNLRSFQNHRSVVAFSVRPLEDMNEITSHMLEVVQAHMALGKPQSTSNAGGAMNINTTPMSRTGMGNMGGMGGMGGGYTGANDMVNNGLSANQNQVLSLIRSCPDPQGISIQDLKQRLSGISIAVIKQAVEFLSNEGHIFSTIDEDHYKSTDNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAL4</accession>
    <name>A0A672YAL4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Immunoglobin superfamily, member 21a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YAL4"/>
    <dbReference type="Ensembl" id="ENSSORT00005000125.1">
        <property type="protein sequence ID" value="ENSSORP00005000110.1"/>
        <property type="gene ID" value="ENSSORG00005000087.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YAL4"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000509">
        <property type="entry name" value="Immunoglobin superfamily member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF121">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="40"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="347"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="464" mass="51956" checksum="5EE0CF8C4D5D2A10" modified="2020-06-17" version="1">FRVVHLFFPCNKTVFTFISFIFFAGYLTVTIEPLPPVVVGETVTLKCNFKTDGRLREIVWYRVTDGGTIKQKIFTYDAMFNTNYSHMEDYRRREDLVYQSTVRLPEVRISDNGPYECHVGIYDRATREKVVLASGNVFLTVMSPPNNISVVAENTPAPFSRYQAQNFTLVCTAKGGKPAPSVYFKRDGELIEVISYTLPTTSEQGGGSAGVRGARPLISRDLDDTKLQRSLSLLDPEGRSARLHTESPQRVHTQGQQAYEPSPATEVIPETVVSREFPRWVHSTDPLYYFSHSHIPQSDGSVVVQARLTWTLNPQLDNDALFSCEVKHPALSMPMQTEVTLAAPKGPKLLMSPTRAKVGDTVRITVQGFQVGTSGNEVFPEPLFTWTRVGGRLLDGSTEREGKELILERVPAELNGSMYRCTAQNPLGSTDTHTRLIVFDCIPNWLNNVFFVRLHLVSVTVTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YAX6</accession>
    <name>A0A672YAX6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Paxillin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115419215</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the paxillin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029989721.1">
        <property type="nucleotide sequence ID" value="XM_030133861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YAX6"/>
    <dbReference type="Ensembl" id="ENSSORT00005000358.1">
        <property type="protein sequence ID" value="ENSSORP00005000335.1"/>
        <property type="gene ID" value="ENSSORG00005000236.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115419215"/>
    <dbReference type="InParanoid" id="A0A672YAX6"/>
    <dbReference type="OrthoDB" id="15567at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09336">
        <property type="entry name" value="LIM1_Paxillin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09337">
        <property type="entry name" value="LIM2_Paxillin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09338">
        <property type="entry name" value="LIM3_Paxillin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09411">
        <property type="entry name" value="LIM4_Paxillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000008">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000009">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000012">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000018">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001904">
        <property type="entry name" value="Paxillin_Lim_dom4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047075">
        <property type="entry name" value="Paxillin_TGFB1I1_LIM_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017305">
        <property type="entry name" value="Tgfb1i1/Leupaxin/TGFB1I1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF64">
        <property type="entry name" value="PAXILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03535">
        <property type="entry name" value="Paxillin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037881">
        <property type="entry name" value="Leupaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 11 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="290"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="350"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="408"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="468"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="29"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="192"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005000335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="525" mass="57937" checksum="1AD0E396981245D4" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YFD7</accession>
    <name>A0A672YFD7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YFD7"/>
    <dbReference type="Ensembl" id="ENSSORT00005002627.1">
        <property type="protein sequence ID" value="ENSSORP00005002561.1"/>
        <property type="gene ID" value="ENSSORG00005001538.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YFD7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF847">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="56"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005002561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="325" mass="37101" checksum="BB590676F81991DF" modified="2020-06-17" version="1">MIAPECCLNVFCLNICVFSVLLYISLSKKNFCHLRLTINYVYFFILFMVYVLIMCSNSIIVYLILIHKNLHEPMYVFIAALSFNSVLFSTVIYPKLLTDFLSEKQIISLSACIFQYFIYYSLASSDFLLLAAMAYDRYVSICKPLQYPTIMRKSTVSILLFLAWLWPAAQVAGGVVVGGFRKLCTFILTGIFCNNSIFKLNCVPSAALAIYGVFGLINFAVIPVLFVLFTYAKIFIITYNKGGEVRKKAAETCLPHLMVLFSFSCMCAYDVIIARLELSFSKTVRLVMSLQMVLYNPLLNPIIYGLKMKEISKHLKNLFSQIRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YHY3</accession>
    <name>A0A672YHY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methyl-CpG-binding domain protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Methyl-CpG-binding protein MBD4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mismatch-specific DNA N-glycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mbd4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with MLH1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YHY3"/>
    <dbReference type="Ensembl" id="ENSSORT00005004275.1">
        <property type="protein sequence ID" value="ENSSORP00005004153.1"/>
        <property type="gene ID" value="ENSSORG00005002530.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YHY3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008263">
        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000051">
        <property type="entry name" value="Methyl-CpG-binding domain protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017352">
        <property type="entry name" value="MBD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045138">
        <property type="entry name" value="MeCP2/MBD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074:SF7">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15074">
        <property type="entry name" value="METHYL-CPG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038005">
        <property type="entry name" value="Methyl_CpG_bd_MBD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MBD" evidence="14">
        <location>
            <begin position="42"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="119"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="244"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="537" mass="61307" checksum="15EC4CE375AA7631" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YIB7</accession>
    <name>A0A672YIB7_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YIB7"/>
    <dbReference type="Ensembl" id="ENSSORT00005004423.1">
        <property type="protein sequence ID" value="ENSSORP00005004294.1"/>
        <property type="gene ID" value="ENSSORG00005002603.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YIB7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="28391" checksum="18A843D6D93F1AB1" modified="2020-06-17" version="1">DAPAPPTAAGVPPIPPGSVREPAPADSMAATELIKGNGPKAGPPVKPPSQGPRSSARHRLLREALCRHSNSPPVECPPEETRDPSPSTSSPRPLFPPTTVILGDSILRHVRFFNTTTKCFPGSTVTSILHELPQLLLSLPSTVTRIIVHVGFNDTSLQQSEVTKVHFKNLFDKLKGSGKAVFISGPLPSFARGVGQFSRLLSLNTWLQSASALNGFSFIDNFNLFWNRPSFYKSDGVHPSRLGSTVLAQNIQHAVLTKTTPTPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJ31</accession>
    <name>A0A672YJ31_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJ31"/>
    <dbReference type="Ensembl" id="ENSSORT00005004741.1">
        <property type="protein sequence ID" value="ENSSORP00005004605.1"/>
        <property type="gene ID" value="ENSSORG00005002803.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJ31"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="97" mass="10743" checksum="0F46B9F752670C0D" modified="2020-06-17" version="1">MNAIAADMCTNARRVVRKSWIPKLKLLMAESDAYTAFLPDGVKTEEDTLGADPAFDPASLEAGAGMEKSAIFAEKRSILKQCYHRTLSHLFLFYGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YJB3</accession>
    <name>A0A672YJB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YJB3"/>
    <dbReference type="Ensembl" id="ENSSORT00005004784.1">
        <property type="protein sequence ID" value="ENSSORP00005004648.1"/>
        <property type="gene ID" value="ENSSORG00005002830.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YJB3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001594">
        <property type="entry name" value="Immunoglobulin heavy variable 9-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005004648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="124" mass="14053" checksum="9E6E00CF2A926CF0" modified="2020-06-17" version="1">QTLTESGSAVKSPGESHRLTCTTSGFTMSNHWMHWVRQAPGKGLEWIAAVDYGGSSKYYSQSVQGRFTISRDNGRNQLYLQMNSLKTEDSAVYYCARDPQRLELVKQLYKILKNASVVVTLYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YKX1</accession>
    <name>A0A672YKX1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="12">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">pim3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="12"/>
    <dbReference type="RefSeq" id="XP_030005371.1">
        <property type="nucleotide sequence ID" value="XM_030149511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YKX1"/>
    <dbReference type="FunCoup" id="A0A672YKX1">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005005380.1">
        <property type="protein sequence ID" value="ENSSORP00005005224.1"/>
        <property type="gene ID" value="ENSSORG00005003122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429791"/>
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    <sequence length="329" mass="37147" checksum="158683A345CF7F82" modified="2020-06-17" version="1">MLLSKFGSFSHICNPSSMDLPVKIQLQPVKMEKEPFEKVYQVGSVLGSGGFGTVYAGSRISDGAPVAVKHVAKERVTEWGTINGAMVPLEILLLKKVSSSFRGVIKLLDYYERADGWLIVMERPELVKDLFDFITEKGALDEDTARGFFRQVLEAVRHCYSCGVVHRDIKDENLLVDLRTGELKLIDFGSGAILKDTVYTDFDGTRVYSPPEWIRFHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLYFRRRVSAECQQLIKWCLALRPSDRPTLEQICDHPWMRATAESPKSPEEPVAGDIRLRTIDTDSSSSTSSSKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YLG0</accession>
    <name>A0A672YLG0_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Zinc finger CCCH domain-containing protein 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">zc3h15</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <text evidence="3">Belongs to the ZC3H15/TMA46 family.</text>
    </comment>
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        <property type="gene ID" value="ENSSORG00005003233.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="ZC3H15/TMA46_C"/>
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        <property type="entry name" value="Znf_CCCH"/>
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        <property type="entry name" value="Znf_CCCH_sf"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
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    </dbReference>
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        <property type="entry name" value="CCCH zinc finger"/>
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    </dbReference>
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        <property type="entry name" value="ZF_C3H1"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
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        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
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            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
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        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="298"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="218"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="392"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00015073"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50103"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="432" mass="49206" checksum="B07B7073EFBFAEFA" modified="2020-06-17" version="1">MPPKKPAPVAGNKKTQEKKKEKIIEDKTFGLKNKKGAKQQKFIKNVTQQVKYGQQSARQIAQAEADKTSKKNDKKKEMDELNELFKPVVTAQKVSKGVDPKSVLCAFFKQGQCTKGDKCKFSHDLSMERKCEKKNMYVDERDEDLEKDTMENWDEKKLEEVVNKKHGEAEKKKAKTQIVCKYFLEAIENNKYGWFWVCPAGGDTCMYRHALPPGFVLKKDKKKEEKEDEVSLEELIENERAALGPNVTRITLETFLAWKKRKRQEKVDKAREEMEKKKADFKAGKSLVVSGREVFEFRPELVDDDDDEADDTRYQNEDDDEYEHEEEIDVTEIQDIDLSRFVPQEVDNTGITVASTDRFASRNKTEPTETEEQVNGACGGTEANGLSGAEGGGEEGGGEDDGEEEVPVDENLFTGEDLEELDEELNTLVLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNJ5</accession>
    <name>A0A672YNJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672YNJ5"/>
    <dbReference type="Ensembl" id="ENSSORT00005006374.1">
        <property type="protein sequence ID" value="ENSSORP00005006123.1"/>
        <property type="gene ID" value="ENSSORG00005003639.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YNJ5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000203">
        <property type="entry name" value="FERM and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221:SF10">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="1">
        <location>
            <begin position="27"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="153" mass="17628" checksum="C14C63554467AA78" modified="2020-06-17" version="1">MALARVFVLQNPKSSIMSGNSLLFLPNVLKVYLENGQTKAFKFDNTTTVKDIVLTLKDKLSIRAIEYFALVLEQQYSITKLLLLHEDELIQKVVQKKDSHDYRCLFRVCFIPRDPVDMLQDDPSAFEYLFLQVRETPSIIVLGTSCRSVLLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YNU3</accession>
    <name>A0A672YNU3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="22">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">nme3</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Major role in the synthesis of nucleoside triphosphates other than ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:44641"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:18114"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 20 21">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="22"/>
    <dbReference type="AlphaFoldDB" id="A0A672YNU3"/>
    <dbReference type="Ensembl" id="ENSSORT00005006501.1">
        <property type="protein sequence ID" value="ENSSORP00005006249.1"/>
        <property type="gene ID" value="ENSSORG00005003707.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YNU3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="23" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="24">
        <location>
            <begin position="35"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="20">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="183" mass="20726" checksum="59B07B16A9B8C1CF" modified="2020-06-17" version="1">MECVYNALWWWVLADVCISLSSCPTYLDLGWTGVNERTFIAVKPDGVQRRLVGEIVRRFEKRGFKLVGLKLVKVSEELLGEHYADLRSKPFFSGLISYMSSGPVVAMVWQGLDVVKTARKMMGETNPGDSLPGTIRGDFCVEVGKNVIHGSDSVESAQKEISLWFRQNELQQWQKCGTNWIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YQH9</accession>
    <name>A0A672YQH9_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fnbp4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_030135897.1"/>
    </dbReference>
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        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005007095.1">
        <property type="protein sequence ID" value="ENSSORP00005006833.1"/>
        <property type="gene ID" value="ENSSORG00005003966.1"/>
    </dbReference>
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    <dbReference type="CTD" id="23360"/>
    <dbReference type="InParanoid" id="A0A672YQH9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Formin binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053076">
        <property type="entry name" value="WW_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46697">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46697:SF1">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
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    </dbReference>
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        <property type="entry name" value="WW_DOMAIN_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="204"/>
        </location>
    </feature>
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            <begin position="533"/>
            <end position="567"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="98"/>
        </location>
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            <end position="149"/>
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            <begin position="18"/>
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    </feature>
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            <begin position="758"/>
            <end position="790"/>
        </location>
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            <begin position="796"/>
            <end position="808"/>
        </location>
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            <begin position="829"/>
            <end position="849"/>
        </location>
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        <location>
            <begin position="850"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="874"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005006833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="883" mass="95997" checksum="53DCE83C8E23F5AE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YS20</accession>
    <name>A0A672YS20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025610504" description="WAP domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="119"/>
        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="2">
        <location>
            <begin position="78"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="119" mass="13006" checksum="720A8FEC12F22AED" modified="2020-06-17" version="1">MDKHWSAVCSFILVLCAFVNVHMDLVEGALKDVQSLKGSYGPCFVLPYECQNDSDCSGNGKCCPIGCGSYCFFTNPGKNRNCTNGSSMCAEYCDHDRQCPGEQKCCRTTCGHACRFSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YSR0</accession>
    <name>A0A672YSR0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pyroglutamylated RFamide peptide 2</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
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    <reference evidence="6" key="1">
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RFamide neuropeptide family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A672YSR0"/>
    <dbReference type="Ensembl" id="ENSSORT00005007822.1">
        <property type="protein sequence ID" value="ENSSORP00005007542.1"/>
        <property type="gene ID" value="ENSSORG00005004295.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031854">
        <property type="term" value="F:orexigenic neuropeptide QRFP receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P518"/>
    </dbReference>
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        <property type="entry name" value="RFamide_26RFa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0027">Amidation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025623294" description="Pyroglutamylated RFamide peptide 2" evidence="5">
        <location>
            <begin position="29"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005007542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17338" checksum="37EB76213D994957" modified="2020-06-17" version="1">MRLCFCPGSSRLAVLALILLCPIPWTVSSYPHPRPPAAAPLPTGDEPDLESALLRFRDAADEPWSTASESEEVLLRSERGDLVGRPLSPFPGGLSVDGLHYPVGETDDGGRRNDALNSIAGGLQAVSREKGGFGFRFGRKRWTERGQKRNLDQDLDRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YUP4</accession>
    <name>A0A672YUP4_9TELE</name>
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        <recommendedName>
            <fullName evidence="10">Sodium-dependent glucose transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mfsd4b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
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        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the major facilitator superfamily.</text>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005008250.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="66918" checksum="A126214D30FC9F17" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YW86</accession>
    <name>A0A672YW86_9TELE</name>
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        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
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                <consortium name="Wellcome Sanger Institute Data Sharing"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXS3</accession>
    <name>A0A672YXS3_9TELE</name>
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        <submittedName>
            <fullName evidence="12">Heterogeneous nuclear ribonucleoprotein M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hnrnpm</name>
    </gene>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YXS3"/>
    <dbReference type="FunCoup" id="A0A672YXS3">
        <property type="interactions" value="1924"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005009648.1">
        <property type="protein sequence ID" value="ENSSORP00005009340.1"/>
        <property type="gene ID" value="ENSSORG00005005117.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YXS3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mRNA processing"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="RRM2_hnRNPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000033">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein M isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein M isoform X1"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RRM_dom"/>
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    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF6">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="41"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="197"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="519"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="596" mass="64042" checksum="91481AE1724580DC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXU6</accession>
    <name>A0A672YXU6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Aquaporin 10b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115411713</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>glycerol(in) = glycerol(out)</text>
            <dbReference type="Rhea" id="RHEA:29675"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672YXU6"/>
    <dbReference type="Ensembl" id="ENSSORT00005009749.1">
        <property type="protein sequence ID" value="ENSSORP00005009433.1"/>
        <property type="gene ID" value="ENSSORG00005004635.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000064">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023275">
        <property type="entry name" value="Aquaporin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF13">
        <property type="entry name" value="AQUAPORIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02015">
        <property type="entry name" value="AQUAPORIN3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="311" mass="33354" checksum="17D520DFAA197DD6" modified="2020-06-17" version="1">MSYTNSELFVSPFCRRTAARTPNSFTMERLLRKCIIRNQLVRECLAECLGLYILIGSGAAAQVITSNGNHGNYLTIQLGSALGALFGILVAHGVSGAHLNVVWSMSLSILGRHPWRKVPLYALSHLLGAFLGAATVYLQYYDAIQVFSGGQLLVAGPNATAAIFCSYPAEHLSFWGGLVDQVVATALLLICCLAVVDRRQGRLPNGLAPVLVGALILAIGLSMGSNCGFPLNPARDLGPRLFTFIAGWGEEVFTAGGGWWWIPTVAPFVGGLLGTVIYELLIEVHHLTTPHEEQSPGPELEEVVVDSGNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YXX6</accession>
    <name>A0A672YXX6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger and BTB domain containing 7C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">zbtb7c</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_030006887.1">
        <property type="nucleotide sequence ID" value="XM_030151027.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030006888.1">
        <property type="nucleotide sequence ID" value="XM_030151028.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_030006889.1">
        <property type="nucleotide sequence ID" value="XM_030151029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YXX6"/>
    <dbReference type="Ensembl" id="ENSSORT00005009705.1">
        <property type="protein sequence ID" value="ENSSORP00005009390.1"/>
        <property type="gene ID" value="ENSSORG00005005153.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430795"/>
    <dbReference type="CTD" id="201501"/>
    <dbReference type="InParanoid" id="A0A672YXX6"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001290">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000043">
        <property type="entry name" value="Zinc finger and BTB domain containing 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000115">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000259">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000572">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF7">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="35"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="515"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="675" mass="75628" checksum="2AD347754FE3D265" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZ42</accession>
    <name>A0A672YZ42_9TELE</name>
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        <recommendedName>
            <fullName evidence="16">Metalloendopeptidase</fullName>
            <ecNumber evidence="16">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC115417946</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSSORP00005010001.1"/>
        <property type="gene ID" value="ENSSORG00005005451.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZ42"/>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0016020">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000009">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="MAM_dom"/>
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        <property type="entry name" value="MATH/TRAF_dom"/>
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    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF882">
        <property type="entry name" value="MEPRIN A SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15 16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 15 16" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 15 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025718409" description="Metalloendopeptidase" evidence="16">
        <location>
            <begin position="21"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="19">
        <location>
            <begin position="45"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="18">
        <location>
            <begin position="244"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005010001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="631" mass="71768" checksum="4B1E6B3BCE403AC2" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZ59</accession>
    <name>A0A672YZ59_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">P-type phospholipid transporter</fullName>
            <ecNumber evidence="5">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A672YZ59"/>
    <dbReference type="Ensembl" id="ENSSORT00005010001.1">
        <property type="protein sequence ID" value="ENSSORP00005009677.1"/>
        <property type="gene ID" value="ENSSORG00005005290.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672YZ59"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140327">
        <property type="term" value="F:flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000012">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF48">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="51"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="21">
        <location>
            <begin position="158"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="717"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="843" mass="97500" checksum="FF5389138831F8B7" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672YZY8</accession>
    <name>A0A672YZY8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anti-proliferative protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">btg3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BTG family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_030154908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672YZY8"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005010272.1">
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        <property type="gene ID" value="ENSSORG00005005423.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115433480"/>
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    <dbReference type="InParanoid" id="A0A672YZY8"/>
    <dbReference type="OrthoDB" id="19928at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.90:FF:000002">
        <property type="entry name" value="BTG anti-proliferation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.90">
        <property type="entry name" value="Anti-proliferative protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002087">
        <property type="entry name" value="Anti_prolifrtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033332">
        <property type="entry name" value="BTG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036054">
        <property type="entry name" value="BTG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22978">
        <property type="entry name" value="B-CELL TRANSLOCATION GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22978:SF6">
        <property type="entry name" value="PROTEIN BTG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07742">
        <property type="entry name" value="BTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00310">
        <property type="entry name" value="ANTIPRLFBTG1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00099">
        <property type="entry name" value="btg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160696">
        <property type="entry name" value="BTG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anti-proliferative protein" evidence="3">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005009942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="234" mass="26730" checksum="DF92390396136980" modified="2020-06-17" version="1">MRKEIAAVVFFLKRLVKKGDKLDSEKVDLFVERLAVALQEKFKGHWYPDNPSKGQAYRCIRVNRFCRQDPELLRACRESSVRYDDLGLPREFSLWVDPGEVCCRYAEHNPCFSVATFSTDDEEDKDVARKVTSALERVTSDYHSGSSSDEESSQCSPVTVPNSPCTSQMMNPSAPSWHPKRPVQGKGHPSPRPQFGFRPRNKPHHALRHLWVPPGYRRGPGYWDAPPNLAHCYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z2S3</accession>
    <name>A0A672Z2S3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fkbp15b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A672Z2S3"/>
    <dbReference type="FunCoup" id="A0A672Z2S3">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005011393.1">
        <property type="protein sequence ID" value="ENSSORP00005011019.1"/>
        <property type="gene ID" value="ENSSORG00005002904.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z2S3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056598">
        <property type="entry name" value="FKBP-15_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44927">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44927:SF1">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23649">
        <property type="entry name" value="FKBP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025680135" description="peptidylprolyl isomerase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="5">
        <location>
            <begin position="186"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="660"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="752"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="665"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="983" mass="110682" checksum="694B3BBB50081B1C" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z4S2</accession>
    <name>A0A672Z4S2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Zgc:172282</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">zgc:172282</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the LRFN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z4S2"/>
    <dbReference type="FunCoup" id="A0A672Z4S2">
        <property type="interactions" value="723"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005012175.1">
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        <property type="gene ID" value="ENSSORG00005006276.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z4S2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000016">
        <property type="entry name" value="Leucine-rich repeat and fibronectin type III domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000019">
        <property type="entry name" value="leucine-rich repeat and fibronectin type III domain-containing protein 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
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    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF20">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025520091" evidence="16">
        <location>
            <begin position="29"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="534"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="298"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="427"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="393"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="573"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="670"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="695"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005011789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="716" mass="78752" checksum="430CD7CF71D29EED" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z681</accession>
    <name>A0A672Z681_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Small ribosomal subunit protein mS27</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">28S ribosomal protein S27, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial ribosomal protein S27</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">mrps27</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the mitochondrion-specific ribosomal protein mS27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030000295.1">
        <property type="nucleotide sequence ID" value="XM_030144435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672Z681"/>
    <dbReference type="Ensembl" id="ENSSORT00005013238.1">
        <property type="protein sequence ID" value="ENSSORP00005012836.1"/>
        <property type="gene ID" value="ENSSORG00005006709.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426363"/>
    <dbReference type="CTD" id="23107"/>
    <dbReference type="InParanoid" id="A0A672Z681"/>
    <dbReference type="OrthoDB" id="19830at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000232">
        <property type="entry name" value="28S ribosomal protein S27, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034913">
        <property type="entry name" value="mS27/PTCD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019266">
        <property type="entry name" value="Ribosomal_mS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21393">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21393:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10037">
        <property type="entry name" value="MRP-S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="repeat" description="PPR" evidence="18">
        <location>
            <begin position="140"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="416" mass="46966" checksum="A07A37FC7861D15A" modified="2020-06-17" version="1">MAASVLKRCVTAAVKVKGFSPSLLGRRWLLSASYTDTKLWEAREKDPQNLAVLANIMDRTYDRNLPVSSLTVSRFVDNISSREEIDQAEYYLYKFRHSPNCWYLRDWTVHSWIRQCLKYDGREKALHTVKNKVQYGIFPDDFTFNLLIDSYIKDGDFKSACSVVEEVMLQEAFDLPSTQILSLYALGSYLATRPQLTVSEERALGASLLICGLMKDDTVGLNAQLLGNALLGKVEMLNGIHAVFKGMPLLWTHGYLGRALAVMEQVAAEPGDVKLSKDILDYVSNILQELSSTADTDSSGDESGGEEEADKKQETVDEEDEAERAKLPQYVSRFKELSSQLESLGKVDTASFQTLVTSLAQQNLEASEKLDIEQYESRVQAWESEKRQLILREKEMKEKAEEEKRERLAAKAAAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z722</accession>
    <name>A0A672Z722_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672Z722"/>
    <dbReference type="Ensembl" id="ENSSORT00005012664.1">
        <property type="protein sequence ID" value="ENSSORP00005012267.1"/>
        <property type="gene ID" value="ENSSORG00005006481.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z722"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF86">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DR ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025401364" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="24"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="119" mass="13363" checksum="A505B93C1BEBC8EF" modified="2020-06-17" version="1">MFNAIALTLLGAICISAQGMYTAPELIVYSRDDVIEQTENTLICFINHFFPPIIKTKWTKNGIEVETEDSFKAFILNPDGTFHLFSTLSFNAKDGDIYGCTVEHEALTEPKTEFFGENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672Z8J6</accession>
    <name>A0A672Z8J6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Indoleamine 2,3-dioxygenase 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ido1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the indoleamine 2,3-dioxygenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672Z8J6"/>
    <dbReference type="Ensembl" id="ENSSORT00005013214.1">
        <property type="protein sequence ID" value="ENSSORP00005012812.1"/>
        <property type="gene ID" value="ENSSORG00005006695.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672Z8J6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033754">
        <property type="term" value="F:indoleamine 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:L-tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.480:FF:000003">
        <property type="entry name" value="Indoleamine 2,3-dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000898">
        <property type="entry name" value="Indolamine_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657:SF4">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01231">
        <property type="entry name" value="IDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2 9" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" description="proximal binding residue" evidence="9">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005012812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="413" mass="46453" checksum="E4627D1C3A95FAAB" modified="2020-06-17" version="1">FSMAAATKDSNAPFCLDSYHVSEEFGFILPQPLEELPPYYQPWMDIALRVPELVHSHELRSCINRMPMLSSEFLQRHRELRLAHLALSVMTMGYVWQEGENDTVEVLPRNLAVPFWEVSQRLGLPPILVHADAVLANWKKRDPQGPFDMENLELLFSLPGGDNTRGFFMVTLLVELAAVPALKSIPTVINGVRCGDTEGVTKALDTISKSLQDMTDALKLMHAYVEPSVFYGIMRIYLSGWKDNPSMPKGLVYEGVQREPMEYSGGSAAESSLLHCFDELLGVKHEGKSAFLTRMRNYMPPAHNQMIQDISMQPSLRNFVQEQPSEHLNQAFERCVTKLVALRSYHLNVVSRFITVPAALARQLRNQSQDSNEDLVSQAPKALEERGTGGSGLMTFLKTVRDQTKQAFLPETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZIK9</accession>
    <name>A0A672ZIK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC115422640</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="9"/>
    <dbReference type="RefSeq" id="XP_029994934.1">
        <property type="nucleotide sequence ID" value="XM_030139074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZIK9"/>
    <dbReference type="Ensembl" id="ENSSORT00005017565.1">
        <property type="protein sequence ID" value="ENSSORP00005017051.1"/>
        <property type="gene ID" value="ENSSORG00005000499.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422640"/>
    <dbReference type="InParanoid" id="A0A672ZIK9"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035519">
        <property type="term" value="P:protein K29-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04021">
        <property type="entry name" value="C2_E3_ubiquitin_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000092">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000002">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000063">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF66">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ITCHY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6 9 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="310"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="342"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="422"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="462"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="15">
        <location>
            <begin position="553"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="152"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10 11">
        <location>
            <position position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="887" mass="100818" checksum="65CCFBA19C9C76F6" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZJN1</accession>
    <name>A0A672ZJN1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZJN1"/>
    <dbReference type="Ensembl" id="ENSSORT00005017198.1">
        <property type="protein sequence ID" value="ENSSORP00005016688.1"/>
        <property type="gene ID" value="ENSSORG00005008444.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZJN1"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025687575" evidence="3">
        <location>
            <begin position="27"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005016688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="155" mass="17274" checksum="58E1725F4F59278D" modified="2020-06-17" version="1">MRLTARCIFGTLLAVSSLSWITAASGEEDFQEDKGFEPCAATLTPEGPCKHEEETTCPYLVTLPLLTINLPMRLRELEQILEDLQKLKDNVDQLRKICADCTVNGVKGKGKLVQTQVPTAGKNEGRVEREEVVDKNHEETETDRNKVDNSKGSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZK10</accession>
    <name>A0A672ZK10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Prenylcysteine oxidase 1</fullName>
            <ecNumber evidence="10">1.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">pcyox1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Prenylcysteine oxidase that cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. Only active against free prenylcysteines and not prenylcysteine residues within prenylated proteins or peptides. Involved in the final step in the degradation of prenylated proteins, by degrading prenylcysteines after the protein has been degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>S-(2E,6E)-farnesyl-L-cysteine + O2 + H2O = (2E,6E)-farnesal + L-cysteine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:30231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15894"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:62141"/>
            <dbReference type="EC" id="1.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:30232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[(2E,6E,10E)-geranylgeranyl]-L-cysteine + O2 + H2O = (2E,6E,10E)-geranylgeranial + L-cysteine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:70407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:189549"/>
            <dbReference type="ChEBI" id="CHEBI:189554"/>
            <dbReference type="EC" id="1.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70408"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an S-polyprenyl-L-cysteine + O2 + H2O = a polyprenal + L-cysteine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:53892"/>
            <dbReference type="Rhea" id="RHEA-COMP:13675"/>
            <dbReference type="Rhea" id="RHEA-COMP:13676"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:137934"/>
            <dbReference type="ChEBI" id="CHEBI:137935"/>
            <dbReference type="EC" id="1.8.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:53893"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the prenylcysteine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.5" evidence="10"/>
    <dbReference type="RefSeq" id="XP_030006462.1">
        <property type="nucleotide sequence ID" value="XM_030150602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZK10"/>
    <dbReference type="Ensembl" id="ENSSORT00005018030.1">
        <property type="protein sequence ID" value="ENSSORP00005017511.1"/>
        <property type="gene ID" value="ENSSORG00005008745.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115430529"/>
    <dbReference type="CTD" id="51449"/>
    <dbReference type="InParanoid" id="A0A672ZK10"/>
    <dbReference type="OrthoDB" id="437369at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001735">
        <property type="term" value="F:prenylcysteine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030327">
        <property type="term" value="P:prenylated protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030328">
        <property type="term" value="P:prenylcysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000081">
        <property type="entry name" value="prenylcysteine oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010795">
        <property type="entry name" value="Prenylcys_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017046">
        <property type="entry name" value="Prenylcysteine_Oxase1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944">
        <property type="entry name" value="FARNESYLCYSTEINE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944:SF3">
        <property type="entry name" value="PRENYLCYSTEINE OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07156">
        <property type="entry name" value="Prenylcys_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036292">
        <property type="entry name" value="Prenylcysteine_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0274">FAD</keyword>
    <keyword evidence="4 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025443026" description="Prenylcysteine oxidase 1" evidence="17">
        <location>
            <begin position="23"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Prenylcysteine lyase" evidence="18">
        <location>
            <begin position="128"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR036292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="509" mass="56557" checksum="4A62E17EF95DB813" modified="2020-06-17" version="1">MSLRSLSLRALLLLGLWQTGRRSLASAPELKEQPKKIAIVGAGIGGTAAAYYLRQEFGPGVKIDVFEPNAIGGRLATVRIGDYEYETGGAIIHPLNLHMKHFIDKLGIPPRKDVSSKMAIFDGKELTFEESDWFIVNFLRLLWRYGFNFLRMQMWVESILDKFMRIYQYQQYGYSFSSVEKLLHAMGGDSFLTLVNQTLEETMIGDGFSQVFINDVVEPITRVNYGQSVRINGFVGAVSLAGADSGLWAVDGGNKRVCSGLLYHSKSELIPAGVTSISVKVRPTKTGSTVNYYEVNYVGESGSAHSLYDIVVIATPLHQGKSDITFSSFSPPIPSHYPGRYHQTVATLVHGLLNVSYLGTTEPASDFTVSDVLTTASKGSIINSLSSLDPVHIPPGYKPGPASQTKVWKVFSPEPLTQEQLQNMFLSWDSVSETQWLAYPTYNPPQRKTPPFILHNRLYYLNAVEWAASAMEMSAISARNLALLAHHRWHGQTSKIDQEDLHTRLRGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZLJ7</accession>
    <name>A0A672ZLJ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tachykinin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZLJ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005018189.1">
        <property type="protein sequence ID" value="ENSSORP00005017669.1"/>
        <property type="gene ID" value="ENSSORG00005008796.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZLJ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013055">
        <property type="entry name" value="Tachy_Neuro_lke_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11250:SF5">
        <property type="entry name" value="PROTACHYKININ-1-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11250">
        <property type="entry name" value="TACHYKININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00267">
        <property type="entry name" value="TACHYKININ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025647018" evidence="7">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005017669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="128" mass="14520" checksum="8A56A16DD8B1B291" modified="2020-06-17" version="1">MDQWKLQLVAVTFCALVKTSEGLWSRVEEDKGLSKDSQDELLDRGLTDAVDTLMKRSKALRFYGLMGKRSGLKKPFKVNRRNKGDTFVGLMGRSISKSLSRIYPSVTTTAFDVLEKSHEEGRIMMSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZMM8</accession>
    <name>A0A672ZMM8_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC115423354</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Galectin"/>
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    </dbReference>
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        <property type="entry name" value="Galectin_CRD"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="7"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="138" mass="15546" checksum="EDFD6BCD042382EC" modified="2020-06-17" version="1">SLTVLQELELKNVALRAGDQLKVKGIILHDAERFQIDLGCDPEDLALHFNPRFNDDIDGTVLVCNSKTAGCWGDEKREIHNPLHKGTDVKIVLKLTGDMFEVELPDGQEVRFPNRADMDVINYVRVKGDLKLTSFKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZPR7</accession>
    <name>A0A672ZPR7_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A672ZPR7"/>
    <dbReference type="Ensembl" id="ENSSORT00005018936.1">
        <property type="protein sequence ID" value="ENSSORP00005018392.1"/>
        <property type="gene ID" value="ENSSORG00005009100.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZPR7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134:SF5">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="384" mass="45721" checksum="22A36C4FC9497025" modified="2020-06-17" version="1">MSGLLWTEDTHTHTHKHTHTHKHTHTNTHTQTHTHTQTHTHKHTHTHKHTHTHKHTHTNTHTHTNTHTQTHTHTQTHTHTQTHTHKHTHTHKHTHTTHTHTQTHTHKHTHTNTHTHTQTHTHKHTHTNTHTQTHTHTQTHTHKHTHKHTQTHTQTHTQTHTHKHTHTNTHHTQHHTHTNTHTHTNTHTHKHTHTTHTHTNTRTHTHTQTHTHINTHTQTHTHTQTHTHTQTHTHTQTHAHTHTHKHTHTHTNTHTNTHTQTHTHKHTHTHTQTHTHTNTHTQTHTHTHKHTHTNTHTHKHTHTNTHTHKHTQTHTHTNTHTQTHTHKHTHTHKHTHTQTHTTHKHTQTHTQHTHTQHTQTHTQTHTHTNTHTQTHKHTHRHTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZQ74</accession>
    <name>A0A672ZQ74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">alg9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_030009980.1">
        <property type="nucleotide sequence ID" value="XM_030154120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZQ74"/>
    <dbReference type="Ensembl" id="ENSSORT00005019090.1">
        <property type="protein sequence ID" value="ENSSORP00005018543.1"/>
        <property type="gene ID" value="ENSSORG00005008388.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115432982"/>
    <dbReference type="CTD" id="79796"/>
    <dbReference type="InParanoid" id="A0A672ZQ74"/>
    <dbReference type="OrthoDB" id="497541at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF2">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE ALG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005018543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="613" mass="70501" checksum="08B7870A98DFF179" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZR75</accession>
    <name>A0A672ZR75_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115414946</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZR75"/>
    <dbReference type="FunCoup" id="A0A672ZR75">
        <property type="interactions" value="1000"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020341.1">
        <property type="protein sequence ID" value="ENSSORP00005019780.1"/>
        <property type="gene ID" value="ENSSORG00005009049.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZR75"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000013">
        <property type="entry name" value="Histone-lysine N-methyltransferase SMYD1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF290">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SMYD2-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="10">
        <location>
            <begin position="52"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="11">
        <location>
            <begin position="97"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="476" mass="53908" checksum="489D009DE10DE173" modified="2020-06-17" version="1">MSVPTTIKCDCEGDALKLGWAATPHFCPGSSTVKVSLEDDTPGGGTMTGSIDGLERFDSPGKGRGLRVTRAFKVGELLFSCPAYTHVLSVRERGCYCEFCFTRKEGLARCGKCRKAFYCNAKCQKADWAMHKLECSAMMAFGENWCPSEMTRLVARILAKKKTQKERCGSEKILPVGEMQSHVEDVDNDKREMNEADIAGLHRFYSKHVEFPDHKDLLTLFSQVACNGFTIEDDELSHLGTAVYPDVALVNHSCLPSVIVTYNGTLAEVRAVQDMKTGDEVLISYIDLLYPTDDRNNRLRESYYFTCDCQECKSKSQDKTKLKVRKRSDPIEPEVINNMVRYARKTIREFRAVKTPPSELLEICQQSLEEMGAVFEDSNVYMLHMMYQAMGVCLYMQDVDGAIRYGEKILKPYGKIYPPYSLNVSSMYLKLGRLYMGMERHSLGVKSLKKAMAIMEVAHGKDHHYLTELRKEITQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZRU7</accession>
    <name>A0A672ZRU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin carboxyl-terminal hydrolase 46</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Deubiquitinating enzyme 46</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin thioesterase 46</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin-specific-processing protease 46</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">usp46</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase C19 family. USP12/USP46 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A672ZRU7"/>
    <dbReference type="FunCoup" id="A0A672ZRU7">
        <property type="interactions" value="1569"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005020560.1">
        <property type="protein sequence ID" value="ENSSORP00005019995.1"/>
        <property type="gene ID" value="ENSSORG00005009596.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZRU7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000112">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF714">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0085">Behavior</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="USP" evidence="16">
        <location>
            <begin position="28"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005019995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="369" mass="42630" checksum="18254EE1FEC55E64" modified="2020-06-17" version="1">QIVLRGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTVADILQEEKKQEKQNGRLKNNGTAVTTEAETENKTEPTWVHDIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFVHTETVGKALNLTPETMCYCRNVCISDCFFLCRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSGDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZTQ3</accession>
    <name>A0A672ZTQ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="35">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="7">2.7.7.49</ecNumber>
            <ecNumber evidence="6">3.1.26.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="39">Gag3-Pol3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="45 46">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="45" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="45" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="45" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="37">Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the genomic RNA-nucleocapsid complex.</text>
    </comment>
    <comment type="function">
        <text evidence="34">Integrase (IN) targets the VLP to the nucleus, where a subparticle preintegration complex (PIC) containing at least integrase and the newly synthesized dsDNA copy of the retrotransposon must transit the nuclear membrane. Once in the nucleus, integrase performs the integration of the dsDNA into the host genome.</text>
    </comment>
    <comment type="function">
        <text evidence="36">Nucleocapsid protein p11 (NC) forms the nucleocore that coats the retro-elements dimeric RNA. Binds these RNAs through its zinc fingers. Promotes primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty3 RNA and initiation of reverse transcription.</text>
    </comment>
    <comment type="function">
        <text evidence="33">Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that catalyzes the conversion of the retro-elements RNA genome into dsDNA within the VLP. The enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA primers. The conversion leads to a linear dsDNA copy of the retrotransposon that includes long terminal repeats (LTRs) at both ends.</text>
    </comment>
    <comment type="function">
        <text evidence="2">The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="38">The protease is a homodimer, whose active site consists of two apposed aspartic acid residues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="7"/>
    <dbReference type="EC" id="3.1.26.4" evidence="6"/>
    <dbReference type="Ensembl" id="ENSSORT00005021278.1">
        <property type="protein sequence ID" value="ENSSORP00005020700.1"/>
        <property type="gene ID" value="ENSSORG00005010063.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZTQ3"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000003">
        <property type="entry name" value="Transposon Tf2-6 polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000026">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="25" id="KW-0229">DNA integration</keyword>
    <keyword evidence="30" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="29" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="27" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="18" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="41" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="32" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="31" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="46" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0688">Ribosomal frameshifting</keyword>
    <keyword evidence="24" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="26" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="41" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="41" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-1188">Viral release from host cell</keyword>
    <keyword evidence="28" id="KW-0917">Virion maturation</keyword>
    <keyword evidence="21" id="KW-0862">Zinc</keyword>
    <keyword evidence="19" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="41">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="41">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="41">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="43">
        <location>
            <begin position="585"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="44">
        <location>
            <begin position="1103"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="42">
        <location>
            <begin position="1404"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="40">
        <location>
            <begin position="1379"/>
            <end position="1400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00025590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00025615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00055265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00055383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00063849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00082890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="42">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="44">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005020700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="1465" mass="164286" checksum="5F5D789284669FDD" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZV58</accession>
    <name>A0A672ZV58_9TELE</name>
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        <submittedName>
            <fullName evidence="12">P2Y purinoceptor 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC115426029</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029999814.1">
        <property type="nucleotide sequence ID" value="XM_030143954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A672ZV58"/>
    <dbReference type="Ensembl" id="ENSSORT00005021679.1">
        <property type="protein sequence ID" value="ENSSORP00005021051.1"/>
        <property type="gene ID" value="ENSSORG00005010278.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115426029"/>
    <dbReference type="InParanoid" id="A0A672ZV58"/>
    <dbReference type="OrthoDB" id="10018446at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF35">
        <property type="entry name" value="P2Y PURINOCEPTOR 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="43"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="337" mass="37521" checksum="00767A20F16FE51F" modified="2020-06-17" version="1">MEPVASMLPPVNGSNDSGLCLGETQHVSITVLMCLVFSVGFLLNAFSLWVFCCRLPCWTTGTTLQFHLALSDAIATPVTPLMAVYFAMGNDWPFGRFLCQVKIALLSSHFYGSTIFLTLISVHRFTAVVHFNKRSYMKQKEFVKQLCAGVWIFLLIQSLVFAVLLPPSKEGANSQCLSIHQKNLTNSYFVINFILFIFGFLIPFVVAAVCYGCLANTLTRLNISTAKGLKVKLKSQRMIGVCLVIFGICFLPLNVIRTVGVIVKKFYARRCHVLQQIETAYYCSWILAGVNSCLDPLLYCFGSTNFRDAFGSLRIRQGEVQTRSDSEMTGNQSNSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A672ZYZ7</accession>
    <name>A0A672ZYZ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Parathyroid hormone 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the parathyroid hormone family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A672ZYZ7"/>
    <dbReference type="Ensembl" id="ENSSORT00005022184.1">
        <property type="protein sequence ID" value="ENSSORP00005021538.1"/>
        <property type="gene ID" value="ENSSORG00005010541.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A672ZYZ7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PTH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001415">
        <property type="entry name" value="PTH/PTH-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17223">
        <property type="entry name" value="PARATHYROID HORMONE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17223:SF0">
        <property type="entry name" value="PARATHYROID HORMONE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01279">
        <property type="entry name" value="Parathyroid"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00335">
        <property type="entry name" value="PARATHYROID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005021538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11222" checksum="506EAC97E19B1916" modified="2020-06-17" version="1">MDKSVAAVTASSSTARYPVLSWVQCCLCAPPPFPSRRAVSEHQLMHDRGRSIQSLKRLIWLSGAIEGLHTPSPNWLFRATSDHIWIQRVELKELEMSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A0T6</accession>
    <name>A0A673A0T6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SET binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">setbp1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_029998571.1">
        <property type="nucleotide sequence ID" value="XM_030142711.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_029998572.1">
        <property type="nucleotide sequence ID" value="XM_030142712.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="A0A673A0T6">
        <property type="interactions" value="772"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023006.1">
        <property type="protein sequence ID" value="ENSSORP00005022347.1"/>
        <property type="gene ID" value="ENSSORG00005010888.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115425284"/>
    <dbReference type="CTD" id="26040"/>
    <dbReference type="InParanoid" id="A0A673A0T6"/>
    <dbReference type="OrthoDB" id="9937744at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147:SF2">
        <property type="entry name" value="SET-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
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    <feature type="region of interest" description="Disordered" evidence="3">
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    <feature type="region of interest" description="Disordered" evidence="3">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1224"/>
            <end position="1256"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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            <begin position="1270"/>
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        </location>
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        <location>
            <begin position="1393"/>
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        </location>
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        <location>
            <begin position="1492"/>
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        </location>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="115"/>
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        </location>
    </feature>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005022347.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1517" mass="167732" checksum="441E0818F7F3D2BA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A1X5</accession>
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        <recommendedName>
            <fullName evidence="9">Cornichon family AMPA receptor auxiliary protein 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="7" type="primary">cnih1</name>
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    <organism evidence="7 8">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the cornichon family.</text>
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    <proteinExistence type="inferred from homology"/>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023485.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23428" checksum="1460F9B29C5B52DD" modified="2020-06-17" version="1">MAFTFAAFCYMLALLLTAALIFFAIWHIIAFDELKTDYKNPIDQCNTLNPLVLPEYLIHFFFCVMFFCAAEWLTLCLNLPLLAYHVWRYMSRPIMSCPGLYDPTTIMNADILAYCQKEGWCKLAFYLLSFFYYLYGYETSFVIFWVRVWVQERTPHICQIRVSVILIPFGGLDSSVGEIKSSSKYSSTTIKPKRINIVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A332</accession>
    <name>A0A673A332_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dual specificity protein phosphatase 22-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dusp22a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 14">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A332"/>
    <dbReference type="FunCoup" id="A0A673A332">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005023739.1">
        <property type="protein sequence ID" value="ENSSORP00005023067.1"/>
        <property type="gene ID" value="ENSSORG00005011179.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A332"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990782">
        <property type="term" value="F:protein tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050860">
        <property type="term" value="P:negative regulation of T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000048">
        <property type="entry name" value="dual specificity protein phosphatase 22 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0269404-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948:SF1">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="11">
        <location>
            <begin position="65"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="208" mass="23289" checksum="75C4978AA439521C" modified="2020-06-17" version="1">MGNGMNKVVDGLYLGNIRDAENRESLSKNGITHILSVYNNAKPVFEDMTYLCIHAADASSQNLLQHFKECISFIHECRLNGGSCLVHCLAGVSRSTTMVVAYLMTVTHYSWEDCLSAVKAVRSFVGPNYGFQQQLQEYQTTRVSEYRAWLQSNFRPSPFNDQEQVGALLSQFTEQQESQRRGPDHRWLNQGVGVCALPSDADDPEASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A5L0</accession>
    <name>A0A673A5L0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fibronectin type III and SPRY domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fsd1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in microtubule organization and stabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomerization is required for binding to microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A5L0"/>
    <dbReference type="FunCoup" id="A0A673A5L0">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005024631.1">
        <property type="protein sequence ID" value="ENSSORP00005023927.1"/>
        <property type="gene ID" value="ENSSORG00005011550.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A5L0"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
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    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
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        <property type="entry name" value="Bbox_C"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF4">
        <property type="entry name" value="FIBRONECTIN TYPE III AND SPRY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="146"/>
            <end position="250"/>
        </location>
    </feature>
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        <location>
            <begin position="250"/>
            <end position="456"/>
        </location>
    </feature>
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        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSSORP00005023927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="475" mass="53598" checksum="0DC905EBAA66EAE2" modified="2020-06-17" version="1">MGDQKDSLRKITHTLAVKNEEISNFICTLKQNLDNLEANSNRVQDDLESEFTSLHSVLDEMKENMVTRIKQERASRTYELQSQLSACSKALESSEELLELANQTLVTMAPAFRLSLKAKASDNMSHLMVDFSQEKEMLQGLKFLPVPATPEIQVSECQVCDNTVTVVWTLPEPDSKIDHYILEHRRTNHEGPPRIREDYPWMVVEGIRVLEYTITGLRFDTRYMTFRVKACNKAVAGEFSEPVTLETHAFTFKLDSSSSHQNLKVEDLSVEWDSSGGKIQDIRKDKNRTNSPMHSPARSALMSPKRAPTTRPGRDRFTAESYTVLADTMIDAGQQYWEVRFDKESKAFAVGVALRSLGKFDQLGKTGASWCIHLNNWLQQSLTAKHNNKARTLDCAIPSSIGVYCNYEEGVLSFYNAKTKQLMHTFKTKFQQPVIPAFMVWNGSFSVVTGLQVPSVVQSGQRKNSNTSSSNASLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6G4</accession>
    <name>A0A673A6G4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A6G4"/>
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        <property type="gene ID" value="ENSSORG00005011964.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="173" mass="19252" checksum="B630B1D861ADA7D4" modified="2020-06-17" version="1">MASKLASCAVGQHMSDESECHKKTYCRITSSMTVCLHHKKLYLDKYEHLQKSCCNPFSLHAKSVKGALRVVGIITTNDSSSKMGKDIKPHQKLCPKCLAKVKARETDTETKSTEEDPSYEPSEDTFSELNKSITALSCSPLKPAKVSSRDTALYAKRKLSEAEHALKRTMVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6J5</accession>
    <name>A0A673A6J5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Glutamate metabotropic receptor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">grm7</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673A6J5"/>
    <dbReference type="Ensembl" id="ENSSORT00005025915.1">
        <property type="protein sequence ID" value="ENSSORP00005025160.1"/>
        <property type="gene ID" value="ENSSORG00005012010.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A6J5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15451">
        <property type="entry name" value="7tmC_mGluR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06376">
        <property type="entry name" value="PBP1_mGluR_groupIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000196">
        <property type="entry name" value="Glutamate metabotropic receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000009">
        <property type="entry name" value="Glutamate receptor, metabotropic 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000001">
        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000176">
        <property type="entry name" value="metabotropic glutamate receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001786">
        <property type="entry name" value="GPCR_3_mGluR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000162">
        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01054">
        <property type="entry name" value="MTABOTROPC4R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025440281" evidence="15">
        <location>
            <begin position="30"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="582"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="620"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="652"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="779"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="821"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="16">
        <location>
            <begin position="582"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="866"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="872"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005025160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="907" mass="101733" checksum="A7D894C4683A4CF5" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A6J9</accession>
    <name>A0A673A6J9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRELI domain containing protein 3B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prelid3b</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673A6J9"/>
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        <property type="protein sequence ID" value="ENSSORP00005024272.1"/>
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    </dbReference>
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        <property type="entry name" value="Slowmo/Ups"/>
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        <property type="entry name" value="MSF1/PX19 RELATED"/>
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    </dbReference>
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        <property type="entry name" value="PRELI_MSF1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005024272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="194" mass="21584" checksum="6BB8F612D710B087" modified="2020-06-17" version="1">MKIWTSEHIFNHPWETVTKAAMQKYPNPMNPSVFGVDVLDRAIDKQGRLHSKRLLSTEWGLPSIVKSIIGNTRTCTYVQEQSVVDPKEKTFELQSTNITFTNMVSVDEKLTYKPHPEDPEKTVLTQEAIISVKGVSLSSYLEGVMANTISANAGKGREAMEWVIRRLNAEIEELAATACGTMRSPMAAAAVTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673A8L2</accession>
    <name>A0A673A8L2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">MAP/microtubule affinity-regulating kinase 3</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">mark4a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1. Phosphorylates PKP2 and KSR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with MAPT/TAU. Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5. Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ. Interacts with PKP2 (via N-terminus). Interacts with CDC25C. Interacts with KSR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A673A8L2"/>
    <dbReference type="Ensembl" id="ENSSORT00005026389.1">
        <property type="protein sequence ID" value="ENSSORP00005025626.1"/>
        <property type="gene ID" value="ENSSORG00005012320.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673A8L2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035556">
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        <source>
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    <sequence length="708" mass="78053" checksum="231C146738C502CA" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
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            <end position="72"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
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        <location>
            <begin position="174"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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    </feature>
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        <location>
            <begin position="296"/>
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    </feature>
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        <location>
            <begin position="233"/>
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    </feature>
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        <location>
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        </location>
    </feature>
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    </feature>
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        <location>
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            <end position="366"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067851"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005026888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="379" mass="41618" checksum="5A068954155F8A45" modified="2020-06-17" version="1">MVKIFIGNLACNTTAEELRELFEKYGKITECDVVKNYGFVHMSSVSEAEEAIRNLNQHQLHGWRMNVEMSKGRPKSTTKLHVGNLGEGVTNDDLRAKFEAFGTVVECDIVKDYAFVHMERVEDAMDAIVKLDNTAFKGKLMNVQLSTSRLRTAPGMGDHTGCYVCGKHGHWSKDCPNNRNGSLSDGPRGGRSSYGLPRGPPGYGRGSYGMATSSSTDYMGGPSYSRASYVGGPPLPPPPPPPPPRRVSGYSSEYTSRGSFLDRPSAPYSSADYYEKFRAPSFGSSYFEDRRMSYIAPPPPPSSSSLSKHSALDPYDHRPPPSTAATAYYAQAGSPIRRVPASTTGYPYEQTRLSPVSSSRSSYSISRTKDHYSARYAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEN2</accession>
    <name>A0A673AEN2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA methyltransferase 10 homolog C</fullName>
            <ecNumber evidence="2">2.1.1.218</ecNumber>
            <ecNumber evidence="3">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mitochondrial ribonuclease P protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">RNA (guanine-9-)-methyltransferase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">mRNA methyladenosine-N(1)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">tRNA (adenine(9)-N(1))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA (guanine(9)-N(1))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">trmt10c</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>adenosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43148"/>
            <dbReference type="Rhea" id="RHEA-COMP:10363"/>
            <dbReference type="Rhea" id="RHEA-COMP:10364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.218"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(1)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55392"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12415"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.218" evidence="2"/>
    <dbReference type="EC" id="2.1.1.221" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673AEN2"/>
    <dbReference type="SMR" id="A0A673AEN2"/>
    <dbReference type="FunCoup" id="A0A673AEN2">
        <property type="interactions" value="1435"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005028883.1">
        <property type="protein sequence ID" value="ENSSORP00005028080.1"/>
        <property type="gene ID" value="ENSSORG00005013451.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AEN2"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160106">
        <property type="term" value="F:tRNA (adenine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097745">
        <property type="term" value="P:mitochondrial tRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18102">
        <property type="entry name" value="Trm10_MRRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.30:FF:000003">
        <property type="entry name" value="tRNA methyltransferase 10C, mitochondrial RNase P subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025812">
        <property type="entry name" value="Trm10_C_MTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF5">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="20">
        <location>
            <begin position="92"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005028080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="360" mass="42425" checksum="AE95FFE2CEE53A0D" modified="2020-06-17" version="1">MLRFITVRDLGVVLKGSNFLIPTTTKRRVISFPPVASACRHWVSIFNRHFHTGNLLRKDVPLDAEEGDGEEGIIWKNTLFLQFWSRTLDKLLGWRTAQAMIFGQPLIFDLSYEANMSKREIENTVSQLMEVEGWNRRATEPYHLHFCNLQPDSYYKQELLKRYGAETWDRLLITSTEKQHVDLFPRERLVYLTADSPNVLRTYDHSKVYIIGGLVDRSIQSGLSLANAKRLKLATARLPLDEFLHWEIGAKNLTLDQMMRIMLTFKETGKWEEALKFVPRRKHDGFHQQQVVGKHDRKSEKTFKSGDYWLYLFLQIQKQSKSKQDFKKVETTLVFILIRQEKHCFKRCINIIKVWFHSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AEV8</accession>
    <name>A0A673AEV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">oprl1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029994413.1">
        <property type="nucleotide sequence ID" value="XM_030138553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AEV8"/>
    <dbReference type="Ensembl" id="ENSSORT00005028621.1">
        <property type="protein sequence ID" value="ENSSORP00005027824.1"/>
        <property type="gene ID" value="ENSSORG00005013317.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115422303"/>
    <dbReference type="CTD" id="4987"/>
    <dbReference type="InParanoid" id="A0A673AEV8"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001626">
        <property type="term" value="F:nociceptin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019233">
        <property type="term" value="P:sensory perception of pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15092">
        <property type="entry name" value="7tmA_NOFQ_opioid_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000014">
        <property type="entry name" value="Kappa-type opioid receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001418">
        <property type="entry name" value="Opioid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF11">
        <property type="entry name" value="NOCICEPTIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00384">
        <property type="entry name" value="OPIOIDR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="60"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="372" mass="41562" checksum="74B1AE2926CEE6DD" modified="2020-06-17" version="1">MDFPEDFLLEELSHLPLYNESLFQNNFSGVYNETDALLPTGVKATIVVVYMIVCVIGLVGNFLVMYVIIRYTKMKTATNIYIFNLALADSLFLATLPFQGTDVFLGFWPFGNTLCKAVVSIDYYNMFTSTFTLTVMSMDRYVAVCHPVKALDMRTPHKAKVVNICVWVLASAFGVPAMVMGNVEEEQEHNSVECIVVLPEPRSYWDPVFGTCVFLFSFLIPVAIISICYSLMVKRLRSVRILSGSKEKDRNLRRITRMVLVVVAAFVVCWTPIQIMVLAQSLGFNLSSLFTLVLMHFCIALGYVNSSLNPVLYAFLDENFKRCFREFCNPSPFRLDTQQSGRMRSIAREVAAAYSCKAGDGGGPGGGTPNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AGE5</accession>
    <name>A0A673AGE5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AGE5"/>
    <dbReference type="Ensembl" id="ENSSORT00005028245.1">
        <property type="protein sequence ID" value="ENSSORP00005027457.1"/>
        <property type="gene ID" value="ENSSORG00005013113.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AGE5"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052752">
        <property type="entry name" value="NACHT-WD_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871">
        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871:SF43">
        <property type="entry name" value="SI:CH211-212K18.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005027457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="78" mass="9252" checksum="29B1E634A73D643D" modified="2020-06-17" version="1">MENVYPLLYLYCKQRGYDFSMVDLRCSVRNPFSEHHDTVQLHVESLQRCQGTLGPNFFVRYCLCYYVVIQKCRSSTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AL56</accession>
    <name>A0A673AL56_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3-N-acetylgalactosaminide6-alpha-sialyltransferase</fullName>
            <ecNumber evidence="19">2.4.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC115429777</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-O-[alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-alpha-D-GalNAc]-L-Ser-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-{alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-[alpha-Neu5Ac-(2-&gt;6)]-alpha-D-GalNAc}-L-seryl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65280"/>
            <dbReference type="Rhea" id="RHEA-COMP:16760"/>
            <dbReference type="Rhea" id="RHEA-COMP:16761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:156395"/>
            <dbReference type="ChEBI" id="CHEBI:156397"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:65281"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>3-O-[alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-alpha-D-GalNAc]-L-Thr-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-{alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-[alpha-Neu5Ac-(2-&gt;6)]-alpha-D-GalNAc}-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65284"/>
            <dbReference type="Rhea" id="RHEA-COMP:16762"/>
            <dbReference type="Rhea" id="RHEA-COMP:16763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:156396"/>
            <dbReference type="ChEBI" id="CHEBI:156398"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:65285"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a ganglioside GM1b (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD1alpha (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41968"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78568"/>
            <dbReference type="ChEBI" id="CHEBI:78569"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:41969"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycolipid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.7" evidence="19"/>
    <dbReference type="RefSeq" id="XP_030005350.1">
        <property type="nucleotide sequence ID" value="XM_030149490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AL56"/>
    <dbReference type="FunCoup" id="A0A673AL56">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005030231.1">
        <property type="protein sequence ID" value="ENSSORP00005029398.1"/>
        <property type="gene ID" value="ENSSORG00005014028.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115429777"/>
    <dbReference type="InParanoid" id="A0A673AL56"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001665">
        <property type="term" value="F:alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047290">
        <property type="term" value="F:alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3-N-acetyl-galactosaminide 6-alpha-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001574">
        <property type="term" value="P:ganglioside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000008">
        <property type="entry name" value="ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45906">
        <property type="entry name" value="ALPHA-N-ACETYL-NEURAMINYL-2,3-BETA-GALACTOSYL-1, 3-N-ACETYL-GALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45906:SF4">
        <property type="entry name" value="ALPHA-N-ACETYL-NEURAMINYL-2,3-BETA-GALACTOSYL-1,3-N-ACETYL-GALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0730">Sialic acid</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025615750" description="alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3-N-acetylgalactosaminide6-alpha-sialyltransferase" evidence="20">
        <location>
            <begin position="28"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005029398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="288" mass="33320" checksum="369AD2DD5D755B84" modified="2020-06-17" version="1">MKSQRLLWVCLLSLSLSLLLWFGHVIRRDSSAPAAHSTGLSGYERITEPRMEQQFLDMHCNQCALISSSGQMLGAGVGEEIDNIECVIRMNNAPTKGYEKDVGSRTSLRVVSHTSVPLLLKNEYYYFQQSADTTYVVWGPERNMRQDGKGRIFNSLLKVAKKYPNVRIYAVTREKVQYCDLVFQNETGKNRMKSGAFLSTGFFTMILLMDMCDSIHVYGMIDDNYCSQANHSTVPYHYYEHNRVKECRMYNVHEHTQRGGHRFITEKAIYARWATRHKMDFKHPSWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AMA9</accession>
    <name>A0A673AMA9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673AMA9"/>
    <dbReference type="Ensembl" id="ENSSORT00005031341.1">
        <property type="protein sequence ID" value="ENSSORP00005030486.1"/>
        <property type="gene ID" value="ENSSORG00005014535.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AMA9"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="145" mass="16185" checksum="A6A36B1CAAE7A686" modified="2020-06-17" version="1">MTQLATQVSQLTRTQAAAAAVSGENQATSPPVRDSAEPNIPAPSKYTGNPDTCRDFFTQLQLIFDSQPVRFSKDSVRIAYVPLILGYPWLQMHEPFIDWGTGEVYSWGKNCETRCKFIKLPECSPKMPQISVAPVSVPMEEDKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ANB7</accession>
    <name>A0A673ANB7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Atlastin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">atl3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673ANB7"/>
    <dbReference type="Ensembl" id="ENSSORT00005030936.1">
        <property type="protein sequence ID" value="ENSSORP00005030093.1"/>
        <property type="gene ID" value="ENSSORG00005014364.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ANB7"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990809">
        <property type="term" value="P:endoplasmic reticulum tubular network membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.420:FF:000001">
        <property type="entry name" value="Atlastin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000314">
        <property type="entry name" value="Atlastin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.420">
        <property type="entry name" value="AHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="13">
        <location>
            <begin position="38"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="513" mass="58440" checksum="C6620F3508FD5308" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AP15</accession>
    <name>A0A673AP15_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">G protein-coupled receptor 141</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AP15"/>
    <dbReference type="Ensembl" id="ENSSORT00005031002.1">
        <property type="protein sequence ID" value="ENSSORP00005030157.1"/>
        <property type="gene ID" value="ENSSORG00005014397.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AP15"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF35">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="25"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005030157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="282" mass="32505" checksum="2A1204B9A1F19867" modified="2020-06-17" version="1">MNFRKNQPSHTLTINNSADYHPFLLSVYIMVLIIGTICLCLTKLTTKPASPTTIAVLNLIFSHFIFLLTVPFRIYYYSVHDWSLGLTFCRVISSMIHVHMYMSFLFYVIIIITRLLDPKHFAILAVLLCGLWCWLLFHVWVSTVYGTKVDLIKSTNGTGHCFRFASNIDFTAKVFNYIVSTIIIVVTVVLTALQAKVFLGIMSKGPLDIEFRSARRSMCFALIMMVSFIPYHIFRLYYLQHLDLENLNEVFLSLTTLNCLDMLTFLGKRSRPCQICFFTVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQ98</accession>
    <name>A0A673AQ98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Interleukin-1 beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC115437597</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IL-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030016744.1">
        <property type="nucleotide sequence ID" value="XM_030160884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673AQ98"/>
    <dbReference type="Ensembl" id="ENSSORT00005032322.1">
        <property type="protein sequence ID" value="ENSSORP00005031444.1"/>
        <property type="gene ID" value="ENSSORG00005014958.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115437597"/>
    <dbReference type="InParanoid" id="A0A673AQ98"/>
    <dbReference type="OrthoDB" id="8962877at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001660">
        <property type="term" value="P:fever generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048246">
        <property type="term" value="P:macrophage chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042119">
        <property type="term" value="P:neutrophil activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00100">
        <property type="entry name" value="beta-trefoil_IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000975">
        <property type="entry name" value="IL-1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078:SF30">
        <property type="entry name" value="INTERLEUKIN-1 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078">
        <property type="entry name" value="INTERLEUKIN-1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00340">
        <property type="entry name" value="IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0497">Mitogen</keyword>
    <keyword evidence="9" id="KW-0666">Pyrogen</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="357" mass="40437" checksum="4BFC66C331CC4F64" modified="2020-06-17" version="1">MDLQESWVKGGILITHDVQEGKHQYEVKNIIKSRRGIGDKSFVRRGDKLLQVNGSDLHDVDPEELAQKLAEGNPMLTVHKAIKKKEHTDSSSPAENVMYPYDKEKMLLNFRMEMTKEEDLGRDEGGVDGTTVEDECKEENGESEQDGDFLIVTMTKTSVSVLAGRSCDTVSPCQECHGVGCNYSDVVMVTESSTVTLVPRGGGSFKRVKVFDASVEHVASHMFLRSLCLQKTVYASPNPEMITIYYYKSDFKKFRGIPVVLNFTGSDCFLKCSKEEERILLQVEVCEKQKLQKISKSDEGTLSFVFYMKGDRTDDRTFESALHLGWFIQIVNTDSVEVTEKERPEEDNHFRVIIQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQE6</accession>
    <name>A0A673AQE6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Junction mediating and regulatory protein, p53 cofactor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC115425408</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AQE6"/>
    <dbReference type="Ensembl" id="ENSSORT00005032378.1">
        <property type="protein sequence ID" value="ENSSORP00005031499.1"/>
        <property type="gene ID" value="ENSSORG00005014993.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQE6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070060">
        <property type="term" value="P:'de novo' actin filament nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072332">
        <property type="term" value="P:intrinsic apoptotic signaling pathway by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031738">
        <property type="entry name" value="JMY/WHAMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031808">
        <property type="entry name" value="JMY/WHAMM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330:SF8">
        <property type="entry name" value="JUNCTION-MEDIATING AND -REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330">
        <property type="entry name" value="P300 TRANSCRIPTIONAL COFACTOR JMY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15871">
        <property type="entry name" value="JMY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15920">
        <property type="entry name" value="WHAMM-JMY_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="8">
        <location>
            <begin position="793"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="654"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="785"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="663"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="681"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="803"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="831"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="844"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="860" mass="98197" checksum="74B183305C2162FC" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AQS6</accession>
    <name>A0A673AQS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hexd</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A673AQS6"/>
    <dbReference type="Ensembl" id="ENSSORT00005032527.1">
        <property type="protein sequence ID" value="ENSSORP00005031644.1"/>
        <property type="gene ID" value="ENSSORG00005015066.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AQS6"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06565">
        <property type="entry name" value="GH20_GcnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038901">
        <property type="entry name" value="HEXDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21040">
        <property type="entry name" value="BCDNA.GH04120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21040:SF6">
        <property type="entry name" value="HEXOSAMINIDASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="5">
        <location>
            <begin position="61"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="482" mass="55311" checksum="FC2072CCBEF18ABF" modified="2020-06-17" version="1">MESLPWPKGQKLVHLDLKGAPPRVEYLHKLIDLFSQLGVNGLLVEYEDMFPYEGELKLLQATTHSSYSREEVLSLQEAAKSKGMEVIPLIQTFGHMEFVLKHRTMCHLREVAHCVGTLNPHKEDGVKLVMEMLRQVVELHPDLHTLHIGADEVYMLGEGEESKLWLASPGQTVEQLFLIHVAKVAKAIKEAWPQMHIIMWDDMMRGMSQDTLNDSGLIRLVEPMLWDYTPNLDVEKTVSLLEKYCSAGMVDVWAASSFKGSTSVHTCVPNTHRHVENHRQWLKVAASVSATVKLKGIALTGWQRYDHLSVLCELIPVALPSLVTCLQLLTDGQFNADTQRKVTEKLGISSVEVENMERRSQGRTLAHLIVDLNSFLNSEDVRYFEKNMFVRGWFSPYHQHRKMVSPLITMQIHRQASTYLTVLQQKVEAVREEMVHFYPDSTVQEWFEEHVSPVLIPLQRIRDDITVLNLWITKSFRFFQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASF0</accession>
    <name>A0A673ASF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">srp54</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). As part of the SRP complex, associates with the SRP receptor (SR) component SRPRA to target secretory proteins to the endoplasmic reticulum membrane. Binds to the signal sequence of presecretory proteins when they emerge from the ribosomes. Displays basal GTPase activity, and stimulates reciprocal GTPase activation of the SR subunit SRPRA. Forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SR subunit SRPRA. SR compaction and GTPase mediated rearrangement of SR drive SRP-mediated cotranslational protein translocation into the ER. Requires the presence of SRP9/SRP14 and/or SRP19 to stably interact with RNA. Plays a role in proliferation and differentiation of granulocytic cells, neutrophils migration capacity and exocrine pancreas development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">The M domain binds the 7SL RNA in presence of SRP19 and binds the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="16">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit SRPRA. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_029982611.1">
        <property type="nucleotide sequence ID" value="XM_030126751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ASF0"/>
    <dbReference type="FunCoup" id="A0A673ASF0">
        <property type="interactions" value="1327"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033492.1">
        <property type="protein sequence ID" value="ENSSORP00005032595.1"/>
        <property type="gene ID" value="ENSSORG00005015472.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115413715"/>
    <dbReference type="CTD" id="6729"/>
    <dbReference type="InParanoid" id="A0A673ASF0"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.30:FF:000002">
        <property type="entry name" value="Signal recognition particle 54 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.140:FF:000003">
        <property type="entry name" value="Signal recognition particle 54 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11 16" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="17">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="504" mass="55800" checksum="797C4D69B27B9226" modified="2020-06-17" version="1">MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCAALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRRMIQHAVFKELVKLVDPGVKAWTPTKGKNNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVVIASEGVEKFKAENFEIIIVDTSGRHKQEDSLFEEMLQVSNAVQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATRSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDRVNELKLDDNEELIDKLKHGQFTLRDMYEQFQNIMKMGPFGQIMGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSKDGAKLFSKQPNRIQRVARGSGVATRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVNPSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ASN1</accession>
    <name>A0A673ASN1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">RLA class II histocompatibility antigen, DP alpha-1 chain-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC115436455</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_030015205.1">
        <property type="nucleotide sequence ID" value="XM_030159345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A673ASN1"/>
    <dbReference type="FunCoup" id="A0A673ASN1">
        <property type="interactions" value="1442"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033226.1">
        <property type="protein sequence ID" value="ENSSORP00005032339.1"/>
        <property type="gene ID" value="ENSSORG00005015338.1"/>
    </dbReference>
    <dbReference type="GeneID" id="115436455"/>
    <dbReference type="InParanoid" id="A0A673ASN1"/>
    <dbReference type="OrthoDB" id="8935021at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002504">
        <property type="term" value="P:antigen processing and presentation of peptide or polysaccharide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05767">
        <property type="entry name" value="IgC1_MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001003">
        <property type="entry name" value="MHC_II_a_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF105">
        <property type="entry name" value="RLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DP ALPHA-1 CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00993">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00920">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0491">MHC II</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025575418" evidence="13">
        <location>
            <begin position="24"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="115"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005032339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="249" mass="27691" checksum="170D3E3DD3A06867" modified="2020-06-17" version="1">MVCRSDMKCSAVIFLIFSTFCACSKIPHEIVYVVGCFVNGSAEVQFEYDGEEMLYVDFQQKEIVYTVPTFLNPDPSLFFTGLSIIRDAEQNGMLCKAATEVAATMLKNIPDKEDPPDSILYSSEEVELGVENSLICFVNNFFPPSINVIWTKNGDVVSEGVSLSRYYPSDSQPLTFRQFSTLTFTPKQGDVYSCTVEHPALERPNTRIWEVDLGHPSIGLDIYCGVGLTVALLGVATGTFLITKRRFGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673ATX3</accession>
    <name>A0A673ATX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear receptor binding factor 2b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nrbf2b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A673ATX3"/>
    <dbReference type="FunCoup" id="A0A673ATX3">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005032661.1">
        <property type="protein sequence ID" value="ENSSORP00005031777.1"/>
        <property type="gene ID" value="ENSSORG00005015120.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673ATX3"/>
    <dbReference type="OrthoDB" id="3694230at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039679">
        <property type="entry name" value="NRBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015056">
        <property type="entry name" value="NRBF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033393">
        <property type="entry name" value="NRBF2_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14964">
        <property type="entry name" value="NUCLEAR RECEPTOR BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14964:SF2">
        <property type="entry name" value="NUCLEAR RECEPTOR-BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08961">
        <property type="entry name" value="NRBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17169">
        <property type="entry name" value="NRBF2_MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140361">
        <property type="entry name" value="MIT domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear receptor-binding factor 2 MIT" evidence="4">
        <location>
            <begin position="26"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear receptor-binding factor 2 C-terminal" evidence="3">
        <location>
            <begin position="145"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005031777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="34412" checksum="048956A92DC6A224" modified="2020-06-17" version="1">MTVRGGERHLSCSGNSGRLDSMEVVDSPLNLAHQQCRKADRLLAAGKYKEAITCHGKAADLLTDAMKTTECEQARLSMELQRDSHVKQQRLIQEQWKRETRRMATKAQADQAQASTNPVLLTQTPSLGLNQSQVSSGPSAAAGPREREYDTLLYQLQTRQAGSCPPLTPLCPGSKTTKDDKTRLEEQQTTIEDLRRLVEHLMDENQRLSAEIERLRSENTRLRSEAVEAVDFVERSELWVLPPAGGAMGTGVGQERKSTGKGKEIAIPQLPPLEMPAQEDLCLDDLPPLELPEDIQNELLQELLDRDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AUU2</accession>
    <name>A0A673AUU2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA/RNP complex-1-interacting phosphatase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual specificity protein phosphatase 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphatase that interacts with RNA/RNP complex 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">dusp11</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Possesses RNA 5'-triphosphatase and diphosphatase activities, but displays a poor protein-tyrosine phosphatase activity. In addition, has phosphatase activity with ATP, ADP and O-methylfluorescein phosphate (in vitro). Binds to RNA. May participate in nuclear mRNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. May interact with SFRS7 and SFRS9/SRP30C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A673AUU2"/>
    <dbReference type="FunCoup" id="A0A673AUU2">
        <property type="interactions" value="567"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSSORT00005033004.1">
        <property type="protein sequence ID" value="ENSSORP00005032117.1"/>
        <property type="gene ID" value="ENSSORG00005015265.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AUU2"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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            <begin position="34"/>
            <end position="187"/>
        </location>
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        <location>
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            <end position="176"/>
        </location>
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            <end position="374"/>
        </location>
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            <end position="250"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00008601"/>
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            <dbReference type="Ensembl" id="ENSSORP00005032117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="374" mass="43356" checksum="DB804EEF115907E9" modified="2020-06-17" version="1">MPPFNKRNGIPDRWLDYKAVGKRLPGTRFIAFKVPLKYSLNCRLPCSDVFGPWELLDILNKENQELGLIIDLTFTTRYYKVQDVPESLLCVKIFTAGHEVPSDATILSFKRAVRRFLRDNSDNDKLIGVHCTHGLNRTGYLICRYLIDVDGMDPKEAVELFNSARGHAMERENYLEDLQSGPKRSNEGIEESEQEPVRGCAEQRPSYTESDLDSREKWQPYSHQSSNHRNSDPRGMDHRSHHRPPYDAPRHSRPLLPLPPPPPLRPPTSAPLPLYQWTPPHPGNEWRSPPHREDRGSRYPPPHLERGPLPPHTQEHWRRGPQPPAPHRHALPRHHAHWTDESYGSDGPGRWERPKMRPPHRYNLRVNPYDSYPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AV69</accession>
    <name>A0A673AV69_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2</fullName>
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        </recommendedName>
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        </alternativeName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
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        </alternativeName>
    </protein>
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        <name evidence="18" type="primary">cmtr2</name>
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        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
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        </lineage>
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            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside)-(ribonucleotide) in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside)-(2'-O-methyl-ribonucleotide) in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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    </comment>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:7-methylguanosine mRNA capping"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Cap methyltransferase 2"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Adrift-typ_MeTrfase"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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    <dbReference type="PANTHER" id="PTHR16121">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16121:SF2">
        <property type="entry name" value="CAP-SPECIFIC MRNA (NUCLEOSIDE-2'-O-)-METHYLTRANSFERASE 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SAM_MT_ADRIFT"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 16" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adrift-type SAM-dependent 2'-O-MTase" evidence="17">
        <location>
            <begin position="128"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="167"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
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        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
    </feature>
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        <location>
            <position position="256"/>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078839"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSSORP00005033521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000472271"/>
        </source>
    </evidence>
    <sequence length="781" mass="86870" checksum="C0B18A738F257FAE" modified="2020-06-17" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2020-06-17" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A673AW39</accession>
    <name>A0A673AW39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">10-formyltetrahydrofolate dehydrogenase</fullName>
            <ecNumber evidence="9">1.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">aldh1l1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Sphaeramia orbicularis</name>
        <name type="common">orbiculate cardinalfish</name>
        <dbReference type="NCBI Taxonomy" id="375764"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Gobiaria</taxon>
            <taxon>Kurtiformes</taxon>
            <taxon>Apogonoidei</taxon>
            <taxon>Apogonidae</taxon>
            <taxon>Apogoninae</taxon>
            <taxon>Sphaeramia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2019-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="Wellcome Sanger Institute Data Sharing"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-10-formyltetrahydrofolate + NADP(+) + H2O = (6S)-5,6,7,8-tetrahydrofolate + CO2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10180"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="1.5.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10181"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">In the N-terminal section; belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.6" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A673AW39"/>
    <dbReference type="Ensembl" id="ENSSORT00005034788.1">
        <property type="protein sequence ID" value="ENSSORP00005033875.1"/>
        <property type="gene ID" value="ENSSORG00005015248.1"/>
    </dbReference>
    <dbReference type="InParanoid" id="A0A673AW39"/>
    <dbReference type="Proteomes" id="UP000472271">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016155">
        <property type="term" value="F:formyltetrahydrofolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009258">
        <property type="term" value="P:10-formyltetrahydrofolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07140">
        <property type="entry name" value="ALDH_F1L_FTFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08703">
        <property type="entry name" value="FDH_Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="C